Journal: Proceedings of the Royal Society B: Biological Sciences

Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

doi: 10.1098/rspb.2017.1134

Figure Lengend Snippet: Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p

Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

Techniques: Activity Assay

Journal: Proceedings of the Royal Society B: Biological Sciences

Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

doi: 10.1098/rspb.2017.1134

Figure Lengend Snippet: GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)

Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

Techniques: Gas Chromatography-Mass Spectrometry

Journal: Proceedings of the Royal Society B: Biological Sciences

Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

doi: 10.1098/rspb.2017.1134

Figure Lengend Snippet: Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi B. bassiana ( b ), Me. anisopliae ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi B. bassiana and Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p

Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

Techniques: Activity Assay

Journal: Proceedings of the Royal Society B: Biological Sciences

Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

doi: 10.1098/rspb.2017.1134

Figure Lengend Snippet: Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test, p

Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

doi: 10.3892/mmr.2018.8455

Figure Lengend Snippet: β-elemene inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P

Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

Techniques: Migration

Journal: Molecular Medicine Reports

Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

doi: 10.3892/mmr.2018.8455

Figure Lengend Snippet: β-elemene induces apoptosis in SiHa cells. (A) Following treatment of SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to detect cellular apoptosis; *P

Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

Techniques: Flow Cytometry, Cytometry

Journal: Molecular Medicine Reports

Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

doi: 10.3892/mmr.2018.8455

Figure Lengend Snippet: β-elemene inhibits the proliferation and survival of SiHa cells. Following treatment of SiHa cells with increasing doses of β-elemene (0–50 µg/ml) for 24, 48 and 72 h, the MTT assay was used to detect the proliferation and survival of SiHa cells. Each test was conducted in triplicate. Values presented represent the mean ± standard deviation of the three independent experiments. *P

Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

Techniques: MTT Assay, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

doi: 10.3892/mmr.2018.8455

Figure Lengend Snippet: β-elemene induces cell cycle arrest in SiHa cells during G1. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to investigate the influence of β-elemene on the progression of the cell cycle; *P

Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

Techniques: Flow Cytometry, Cytometry

Journal: Molecular Medicine Reports

Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

doi: 10.3892/mmr.2018.8455

Figure Lengend Snippet: β-elemene suppresses the Wnt/β-catenin signaling pathway. Expression levels of β-catenin, TCF7, and c-Myc were detected via western blot analysis. Each experiment was performed in triplicate. Values presented represent the mean ± standard deviation of three independent experiments. *P

Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

Techniques: Expressing, Western Blot, Standard Deviation

Journal: Oncology Reports

Article Title: β-elemene inhibits the generation of peritoneum effusion in pancreatic cancer via suppression of the HIF1A-VEGFA pathway based on network pharmacology

doi: 10.3892/or.2019.7360

Figure Lengend Snippet: Thirty-three target genes for β-elemene against pancreatic cancer. (A) Venn diagram revealing the overlapping target genes for β-elemene against pancreatic cancer. (B) Network of target genes for β-elemene against pancreatic cancer. Red diamond represents β-elemene; red hexagon represents pancreatic cancer; blue circles represent target genes of both β-elemene and pancreatic cancer; yellow triangles represent KEGG pathway analysis of 33 target genes based on the DAVID database. KEGG, Kyoto encyclopedia of genes and genomes; DAVID, Database for Annotation, Visualization and Integrated Discovery.

Article Snippet: Serial concentrations of β-elemene [CSPC Pharmaceutical Group Company Ltd. (YUANDA), Dalian, China] were added to the cells: 0, 0.5, 1, 2, 4, 8 and 16 µM.

Techniques:

Journal: Oncology Reports

Article Title: β-elemene inhibits the generation of peritoneum effusion in pancreatic cancer via suppression of the HIF1A-VEGFA pathway based on network pharmacology

doi: 10.3892/or.2019.7360

Figure Lengend Snippet: Network of β-elemene-target genes. Red diamond represents β-elemene; blue circles represent target genes of β-elemene.

Article Snippet: Serial concentrations of β-elemene [CSPC Pharmaceutical Group Company Ltd. (YUANDA), Dalian, China] were added to the cells: 0, 0.5, 1, 2, 4, 8 and 16 µM.

Techniques:

Journal: Oncology Reports

Article Title: β-elemene inhibits the generation of peritoneum effusion in pancreatic cancer via suppression of the HIF1A-VEGFA pathway based on network pharmacology

doi: 10.3892/or.2019.7360

Figure Lengend Snippet: Antitumor effect of β-elemene in pancreatic cancer. (A) MTT assay revealed that cell viability was suppressed by β-elemene in PANC-1 cells in a dose-dependent manner. (B) MTT assay revealed that cell viability was suppressed by β-elemene in BxPC3 cells in a dose-dependent manner. (C and D) MTT assay revealed that cell viability was suppressed by β-elemene in cells isolated from peritoneum effusion of pancreatic cancer patients in a dose-dependent manner.

Article Snippet: Serial concentrations of β-elemene [CSPC Pharmaceutical Group Company Ltd. (YUANDA), Dalian, China] were added to the cells: 0, 0.5, 1, 2, 4, 8 and 16 µM.

Techniques: MTT Assay, Isolation

Journal: Oncology Reports

Article Title: β-elemene inhibits the generation of peritoneum effusion in pancreatic cancer via suppression of the HIF1A-VEGFA pathway based on network pharmacology

doi: 10.3892/or.2019.7360

Figure Lengend Snippet: Validation of the HIF1A-VEGFA pathway inhibited by β-elemene in pancreatic cancer. (A) Western blot analysis in PANC-1 and BxPC3 pancreatic cancer cell lines and (B) corresponding histogram of protein expression levels of HIF1A and VEGFA in PANC-1 and BxPC3 pancreatic cancer cells. Three independent biological replicates were performed and data are presented as the mean ± standard deviation. *P

Article Snippet: Serial concentrations of β-elemene [CSPC Pharmaceutical Group Company Ltd. (YUANDA), Dalian, China] were added to the cells: 0, 0.5, 1, 2, 4, 8 and 16 µM.

Techniques: Western Blot, Expressing, Standard Deviation

Journal: Oncology Reports

Article Title: β-elemene inhibits the generation of peritoneum effusion in pancreatic cancer via suppression of the HIF1A-VEGFA pathway based on network pharmacology

doi: 10.3892/or.2019.7360

Figure Lengend Snippet: Molecular docking simulation of β-elemene to HIF1A. Sticks represent the three-dimensional structure of β-elemene; surface represents the protein structure of HIF1A. HIF1A, hypoxia-inducible factor 1-α.

Article Snippet: Serial concentrations of β-elemene [CSPC Pharmaceutical Group Company Ltd. (YUANDA), Dalian, China] were added to the cells: 0, 0.5, 1, 2, 4, 8 and 16 µM.

Techniques: