β-carotene Search Results


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  • 99
    Millipore β carotene
    Impact of carrier oil type on the bioaccessibility of <t>beta‐carotene</t> in nanoemulsions. Different lowercase letters mean significant difference between various carrier oils ( p
    β Carotene, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β carotene/product/Millipore
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    99
    Millipore β carotene standard
    Impact of carrier oil type on the bioaccessibility of <t>beta‐carotene</t> in nanoemulsions. Different lowercase letters mean significant difference between various carrier oils ( p
    β Carotene Standard, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β carotene standard/product/Millipore
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    97
    Millipore all trans β carotene
    Reverse phase HPLC chromatogram showing the carotenoids extracted from P. rhodozyma cells grown with (+ Cu culture) and without Cu +2 (− Cu culture). The yeast cells were recovered at 30 and 72 h, just at the onset of ethanol accumulation and after the exhaustion of ethanol, respectively. Peak 1 represents astaxanthin in all instances. Peak 8 in − Cu, 30 h; peak 10 in + Cu, 30 h and peak 9 in + Cu, 72 h correspond to <t>β-carotene.</t> All other peaks correspond to intermediary unknown carotenoids. Peak detection wavelength was at 480 nm using a diode arrangement detector
    All Trans β Carotene, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    FUJIFILM β carotene
    Effect of <t>β-carotene</t> (BC) on mRNA expression of VEGF in SK-Hep-1, PC-2 and B16F10 cells. Cells were incubated with BC (0–20 μM) for 6 h. Data (means ± SD) are from three or four separate experiments; * P
    β Carotene, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore trans β apo 8 carotenal
    Effect of <t>β-carotene</t> (BC) on mRNA expression of VEGF in SK-Hep-1, PC-2 and B16F10 cells. Cells were incubated with BC (0–20 μM) for 6 h. Data (means ± SD) are from three or four separate experiments; * P
    Trans β Apo 8 Carotenal, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    BASF β carotene
    Expression of ferret CMO2 in SF9 insect cells and identification of cleavage product from <t>β-carotene</t> by HPLC analysis Ferret CMO2 was expressed in SF9 insect cells by baculovirus as described under “Experimental Procedures.” Upper panel, the cell lysates from uninfected ( lane 1 ) and ferret CMO2 baculovirus-infected ( lane 2 ) insect cells were boiled in reducing sample buffer and subjected to 10% SDS-PAGE. The protein expression in insect cells can be detected by Coomassie Blue stain ( A, upper panel ), and the proteins were also transferred to polyvinylidene difluoride membrane and detected with CMO2-specific polyclonal antibody ( B, upper panel ). Lower panel, HPLC profile of the cleavage products of all- trans -β-carotene by ferret CMO2 enzyme. All- trans -β-carotene (3 μM) was incubated with the homogenates from either uninfected ( A ) or ferret CMO2 baculovirus-infected ( B ) insect cells for 1 h at 37 °C as described under “Experimental Procedures.” The cleavage products extracted from the incubation mixture were separated by reverse phase HPLC using a C18 column. A peak corresponding to authentic β-apo-10′-carotenal standard ( C ) was detected at 450 nm only in the incubation mixture with the homogenates of ferret CMO2 baculovirus-infected cells ( B ) but not in that of uninfected cells ( A ). D , spectral analysis of the cleavage product ( blue line ) of all- trans -β-carotene versus β -apo-10′-carotenal standard ( red line ). Both the retention time and absorption spectrum of the product from all- trans -β-carotene matched exactly with that of standard β-apo-10′-carotenal.
    β Carotene, supplied by BASF, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    HiMedia Laboratories β carotene
    Densitogram showing peak area of <t>β-carotene.</t> (a) Peak area of β-carotene before 2,2-diphenyl-1-picrylhydrazyl (DPPH) derivatization, (b) peak area of β-carotene after DPPH derivatization
    β Carotene, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck & Co β carotene
    Antioxidant activity of M. cajuput flower and leaf extracts determined by <t>β-carotene</t> bleaching test
    β Carotene, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β carotene/product/Merck & Co
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    99
    Millipore β carotene linoleic acid
    Antioxidant activity of M. cajuput flower and leaf extracts determined by <t>β-carotene</t> bleaching test
    β Carotene Linoleic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore hplc grade β carotene
    Antioxidant activity of M. cajuput flower and leaf extracts determined by <t>β-carotene</t> bleaching test
    Hplc Grade β Carotene, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Valiant β carotene
    Response surface model plot showing the effects of independent variables on <t>β-carotene</t> content: panel A temperature and power; panel B temperature and time; and panel C power and time.
    β Carotene, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    DSM NV β carotene
    Analysis of NinaB’s enzymatic action on 20, <t>20’-di-nor-β-carotene.</t> (A) HPLC profiles at 360 nm of lipid extracts from in vitro tests for enzymatic activity from NinaB cell lysate (dashed trace) and control (solid trace). (B) Schematic depicting of the catalytic action on 20, 20’-di-nor-β-carotene by NinaB. (C) UV/Vis spectra of the corresponding products and the substrate in (A).
    β Carotene, supplied by DSM NV, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    TCI Tokyo Chemical Industry β carotene
    Effect (mean±S.E.) of Cry1Ac and carotenoids on CAT, SOD, GST activity on Ostrinia nubilalis larvae. Enzyme activity in the fifth stage larvae fed on a) diets made with grain of different maize lines (4Bt, HC, M37W, and 4BtxHC) b) diets made with different amounts of added <t>β-carotene.</t> In (a) the treatments were M37W, HC, 4Bt and 4BtxHC diets, while in (b), different quantities of β-carotene (0.6, 6, and 60 mg/100 mg of diet) were added to 4Bt and M37W diets (4Bt, 4Bt+0.6, 4Bt+6, 4Bt+60, M37W, M37W+6). Fifth stage larvae were used and results are shown two days after exposure. CAT: Catalase; SOD: Superoxide dismutase; GST: Glutathione S-transferase. Different letters indicate statistically significant differences between diets (P
    β Carotene, supplied by TCI Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher β carotene
    ( a ) Time dependence of ESA (λ det = 550 nm) of <t>β-carotene</t> in octane (squares) observed after TPE (λ exc = 1,310 nm). Monoexponential fit: 9 ± 0.2 ps (solid line). ( b ) Power dependence of ESA. Power fit: Exponent = 2.2 ± 0.3 (solid line). ( c ) Relative intensity of ESA excited with linear and circular polarized light (Ω = 0.84 ± 0.07). The “coherence spike” is very large in the two-photon experiments (off scale) and has a quadratic dependence on the excitation power and a linear dependence on the Car concentration. No spike was seen with a pure buffer sample. In experiments with chlorophyll a , which has no two-photon allowed states, the coherence spike was found to be substantially smaller in magnitude (data not shown).
    β Carotene, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Impact of carrier oil type on the bioaccessibility of beta‐carotene in nanoemulsions. Different lowercase letters mean significant difference between various carrier oils ( p

    Journal: Food Science & Nutrition

    Article Title: Stability and in vitro digestibility of beta‐carotene in nanoemulsions fabricated with different carrier oils, et al. Stability and in vitro digestibility of beta‐carotene in nanoemulsions fabricated with different carrier oils

    doi: 10.1002/fsn3.862

    Figure Lengend Snippet: Impact of carrier oil type on the bioaccessibility of beta‐carotene in nanoemulsions. Different lowercase letters mean significant difference between various carrier oils ( p

    Article Snippet: Beta‐carotene (97%, #22040), beta‐carotene standards (PHR1239), pepsin (porcine, P6887), bile extract (porcine, P8361), and pancreatin (porcine pancreas, P7545) were from Sigma‐Aldrich (St. Louis, MO).

    Techniques:

    Impact of carrier oil type on the particle size and beta‐carotene retention of nanoemulsion at different temperatures. Changes of particle size and retention rates at 25°C (a) and 55°C (b) during storage

    Journal: Food Science & Nutrition

    Article Title: Stability and in vitro digestibility of beta‐carotene in nanoemulsions fabricated with different carrier oils, et al. Stability and in vitro digestibility of beta‐carotene in nanoemulsions fabricated with different carrier oils

    doi: 10.1002/fsn3.862

    Figure Lengend Snippet: Impact of carrier oil type on the particle size and beta‐carotene retention of nanoemulsion at different temperatures. Changes of particle size and retention rates at 25°C (a) and 55°C (b) during storage

    Article Snippet: Beta‐carotene (97%, #22040), beta‐carotene standards (PHR1239), pepsin (porcine, P6887), bile extract (porcine, P8361), and pancreatin (porcine pancreas, P7545) were from Sigma‐Aldrich (St. Louis, MO).

    Techniques:

    HPLC chromatograms (UV, 450 nm) of carotenoids in leaf tissues of A. multiflora . 1 all-E-violaxanthin (RT: 6.6); 2 9′-Z-neoxanthin (RT: 7.5); 3 all-E-lutein (RT: 12.5); 4 all-E-zeaxanthin (RT: 14.5); 5 all-E-β-cryptoxanthin (RT: 22.7); 6 α-carotene (RT: 29.3); and 7 All-E-β-carotene (RT: 32.5); Chl chlorophyll

    Journal: 3 Biotech

    Article Title: In vitro propagation, carotenoid, fatty acid and tocopherol content of Ajuga multiflora Bunge

    doi: 10.1007/s13205-016-0376-z

    Figure Lengend Snippet: HPLC chromatograms (UV, 450 nm) of carotenoids in leaf tissues of A. multiflora . 1 all-E-violaxanthin (RT: 6.6); 2 9′-Z-neoxanthin (RT: 7.5); 3 all-E-lutein (RT: 12.5); 4 all-E-zeaxanthin (RT: 14.5); 5 all-E-β-cryptoxanthin (RT: 22.7); 6 α-carotene (RT: 29.3); and 7 All-E-β-carotene (RT: 32.5); Chl chlorophyll

    Article Snippet: All-E-β-cryptoxanthin, all-E-β-carotene and tocopherol standards (δ, γ, and α-tocopherol) were purchased from Sigma Aldrich, St. Louis, MO, USA.

    Techniques: High Performance Liquid Chromatography

    Mapping and QTL analysis for carrot β-carotene content. (A) Manhattan plot for β-carotene concentration. Horizontal line indicates Bonferroni correction for multiple testing. (B) QTL analysis for β-carotene concentration. Horizontal line indicates QTL significance threshold. (C) Linkage map of the distal end of chromosome 7 showing the QTL for β-carotene content. Black bar covers the 1.5 LOD drop off region. The most significant marker, S7_33,825,289, is highlighted in red. (D) Marker effect plot for S7_33,825,289, within the QTL region on chromosome 7. AA represents the homozygous recessive genotype ( y2y2 ), while AB and BB represent the heterozygous and homozygous dominant genotypes ( Y 2 _ ), respectively. Vertical axis indicates the quantity of beta-carotene (μg/g).

    Journal: G3: Genes|Genomes|Genetics

    Article Title: Fine Mapping, Transcriptome Analysis, and Marker Development for Y2, the Gene That Conditions β-Carotene Accumulation in Carrot (Daucus carota L.)

    doi: 10.1534/g3.117.043067

    Figure Lengend Snippet: Mapping and QTL analysis for carrot β-carotene content. (A) Manhattan plot for β-carotene concentration. Horizontal line indicates Bonferroni correction for multiple testing. (B) QTL analysis for β-carotene concentration. Horizontal line indicates QTL significance threshold. (C) Linkage map of the distal end of chromosome 7 showing the QTL for β-carotene content. Black bar covers the 1.5 LOD drop off region. The most significant marker, S7_33,825,289, is highlighted in red. (D) Marker effect plot for S7_33,825,289, within the QTL region on chromosome 7. AA represents the homozygous recessive genotype ( y2y2 ), while AB and BB represent the heterozygous and homozygous dominant genotypes ( Y 2 _ ), respectively. Vertical axis indicates the quantity of beta-carotene (μg/g).

    Article Snippet: Synthetic β-carotene (Sigma-Aldrich, St. Louis, MO) was used in each independent run as a reference standard for calibration. β-Carotene was quantified by absorbance at 450 nm.

    Techniques: Concentration Assay, Marker

    Chemical structure of β-carotene of Dunaliella salina .

    Journal: Toxicology Reports

    Article Title: Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats

    doi: 10.1016/j.toxrep.2019.10.017

    Figure Lengend Snippet: Chemical structure of β-carotene of Dunaliella salina .

    Article Snippet: 3.2 Preparation of standard curve Solutions of β-carotene in the range of 0.02-0.1 mg of standard β-carotene (purchased from Sigma-Aldrich, Germany) were prepared.

    Techniques:

    Reverse phase HPLC chromatogram showing the carotenoids extracted from P. rhodozyma cells grown with (+ Cu culture) and without Cu +2 (− Cu culture). The yeast cells were recovered at 30 and 72 h, just at the onset of ethanol accumulation and after the exhaustion of ethanol, respectively. Peak 1 represents astaxanthin in all instances. Peak 8 in − Cu, 30 h; peak 10 in + Cu, 30 h and peak 9 in + Cu, 72 h correspond to β-carotene. All other peaks correspond to intermediary unknown carotenoids. Peak detection wavelength was at 480 nm using a diode arrangement detector

    Journal: Microbial Cell Factories

    Article Title: A common mechanism explains the induction of aerobic fermentation and adaptive antioxidant response in Phaffia rhodozyma

    doi: 10.1186/s12934-018-0898-7

    Figure Lengend Snippet: Reverse phase HPLC chromatogram showing the carotenoids extracted from P. rhodozyma cells grown with (+ Cu culture) and without Cu +2 (− Cu culture). The yeast cells were recovered at 30 and 72 h, just at the onset of ethanol accumulation and after the exhaustion of ethanol, respectively. Peak 1 represents astaxanthin in all instances. Peak 8 in − Cu, 30 h; peak 10 in + Cu, 30 h and peak 9 in + Cu, 72 h correspond to β-carotene. All other peaks correspond to intermediary unknown carotenoids. Peak detection wavelength was at 480 nm using a diode arrangement detector

    Article Snippet: The peaks derived from the samples were identified by comparison to the spectra and retention time from astaxanthin and β-carotene standards (Sigma-Aldrich).

    Techniques: High Performance Liquid Chromatography

    Effect of β-carotene (BC) on mRNA expression of VEGF in SK-Hep-1, PC-2 and B16F10 cells. Cells were incubated with BC (0–20 μM) for 6 h. Data (means ± SD) are from three or four separate experiments; * P

    Journal: Molecules

    Article Title: Diverse Effects of β-Carotene on Secretion and Expression of VEGF in Human Hepatocarcinoma and Prostate Tumor Cells

    doi: 10.3390/molecules17043981

    Figure Lengend Snippet: Effect of β-carotene (BC) on mRNA expression of VEGF in SK-Hep-1, PC-2 and B16F10 cells. Cells were incubated with BC (0–20 μM) for 6 h. Data (means ± SD) are from three or four separate experiments; * P

    Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM), RPMI1640, nonessential amino acid, penicillin/streptomycin, sodium pyruvate, fetal bovine serum (FBS) and trypsin were obtained from Gibco/BRL (Grand Island, NY, USA). β-Carotene was purchased from Wako (Osaka, Japan), and soluble in THF/BHT to form stock solution of 10 mM, which was diluted with THF at 1:1, 1:3, 1:19 and 1:39 ratio, and then diluted with FBS at 1:9 ratio [ ].

    Techniques: Expressing, Incubation

    Effect of β-carotene (BC) on VEGF secretion in SK-Hep-1, PC-3 and B16F10 cells. Cells were incubated with BC (0–20 μM) for 6 and 12 h. ( A ) 6 h; ( B ) 12 h. Data (means ± SD) are from three or four separate experiments; * P

    Journal: Molecules

    Article Title: Diverse Effects of β-Carotene on Secretion and Expression of VEGF in Human Hepatocarcinoma and Prostate Tumor Cells

    doi: 10.3390/molecules17043981

    Figure Lengend Snippet: Effect of β-carotene (BC) on VEGF secretion in SK-Hep-1, PC-3 and B16F10 cells. Cells were incubated with BC (0–20 μM) for 6 and 12 h. ( A ) 6 h; ( B ) 12 h. Data (means ± SD) are from three or four separate experiments; * P

    Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM), RPMI1640, nonessential amino acid, penicillin/streptomycin, sodium pyruvate, fetal bovine serum (FBS) and trypsin were obtained from Gibco/BRL (Grand Island, NY, USA). β-Carotene was purchased from Wako (Osaka, Japan), and soluble in THF/BHT to form stock solution of 10 mM, which was diluted with THF at 1:1, 1:3, 1:19 and 1:39 ratio, and then diluted with FBS at 1:9 ratio [ ].

    Techniques: Incubation

    Effect of β-carotene (BC) on cell proliferation of SK-Hep-1, PC-3 and B16F10 cells. Cells were incubated with BC (0–20 µM) for 6 and 12 h. Data (means ± SD) are from three or four separate experiments; means for each cell type measured at the same incubation time without a common alphabetic letter differ significantly ( P

    Journal: Molecules

    Article Title: Diverse Effects of β-Carotene on Secretion and Expression of VEGF in Human Hepatocarcinoma and Prostate Tumor Cells

    doi: 10.3390/molecules17043981

    Figure Lengend Snippet: Effect of β-carotene (BC) on cell proliferation of SK-Hep-1, PC-3 and B16F10 cells. Cells were incubated with BC (0–20 µM) for 6 and 12 h. Data (means ± SD) are from three or four separate experiments; means for each cell type measured at the same incubation time without a common alphabetic letter differ significantly ( P

    Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM), RPMI1640, nonessential amino acid, penicillin/streptomycin, sodium pyruvate, fetal bovine serum (FBS) and trypsin were obtained from Gibco/BRL (Grand Island, NY, USA). β-Carotene was purchased from Wako (Osaka, Japan), and soluble in THF/BHT to form stock solution of 10 mM, which was diluted with THF at 1:1, 1:3, 1:19 and 1:39 ratio, and then diluted with FBS at 1:9 ratio [ ].

    Techniques: Incubation

    Effect of β-carotene (BC) on protein expression of VEGF in SK-Hep-1, PC-3 and B16F10 cells. Cells were incubated with BC (0–20 μM) for 6 h. Data (means ± SD) are from three or four separate experiments; * P

    Journal: Molecules

    Article Title: Diverse Effects of β-Carotene on Secretion and Expression of VEGF in Human Hepatocarcinoma and Prostate Tumor Cells

    doi: 10.3390/molecules17043981

    Figure Lengend Snippet: Effect of β-carotene (BC) on protein expression of VEGF in SK-Hep-1, PC-3 and B16F10 cells. Cells were incubated with BC (0–20 μM) for 6 h. Data (means ± SD) are from three or four separate experiments; * P

    Article Snippet: Dulbecco’s Modified Eagle Medium (DMEM), RPMI1640, nonessential amino acid, penicillin/streptomycin, sodium pyruvate, fetal bovine serum (FBS) and trypsin were obtained from Gibco/BRL (Grand Island, NY, USA). β-Carotene was purchased from Wako (Osaka, Japan), and soluble in THF/BHT to form stock solution of 10 mM, which was diluted with THF at 1:1, 1:3, 1:19 and 1:39 ratio, and then diluted with FBS at 1:9 ratio [ ].

    Techniques: Expressing, Incubation

    Expression of ferret CMO2 in SF9 insect cells and identification of cleavage product from β-carotene by HPLC analysis Ferret CMO2 was expressed in SF9 insect cells by baculovirus as described under “Experimental Procedures.” Upper panel, the cell lysates from uninfected ( lane 1 ) and ferret CMO2 baculovirus-infected ( lane 2 ) insect cells were boiled in reducing sample buffer and subjected to 10% SDS-PAGE. The protein expression in insect cells can be detected by Coomassie Blue stain ( A, upper panel ), and the proteins were also transferred to polyvinylidene difluoride membrane and detected with CMO2-specific polyclonal antibody ( B, upper panel ). Lower panel, HPLC profile of the cleavage products of all- trans -β-carotene by ferret CMO2 enzyme. All- trans -β-carotene (3 μM) was incubated with the homogenates from either uninfected ( A ) or ferret CMO2 baculovirus-infected ( B ) insect cells for 1 h at 37 °C as described under “Experimental Procedures.” The cleavage products extracted from the incubation mixture were separated by reverse phase HPLC using a C18 column. A peak corresponding to authentic β-apo-10′-carotenal standard ( C ) was detected at 450 nm only in the incubation mixture with the homogenates of ferret CMO2 baculovirus-infected cells ( B ) but not in that of uninfected cells ( A ). D , spectral analysis of the cleavage product ( blue line ) of all- trans -β-carotene versus β -apo-10′-carotenal standard ( red line ). Both the retention time and absorption spectrum of the product from all- trans -β-carotene matched exactly with that of standard β-apo-10′-carotenal.

    Journal: The Journal of biological chemistry

    Article Title: The Biochemical Characterization of Ferret Carotene-9?, 10?-Monooxygenase Catalyzing Cleavage of Carotenoids in Vitro and in Vivo*

    doi: 10.1074/jbc.M512095200

    Figure Lengend Snippet: Expression of ferret CMO2 in SF9 insect cells and identification of cleavage product from β-carotene by HPLC analysis Ferret CMO2 was expressed in SF9 insect cells by baculovirus as described under “Experimental Procedures.” Upper panel, the cell lysates from uninfected ( lane 1 ) and ferret CMO2 baculovirus-infected ( lane 2 ) insect cells were boiled in reducing sample buffer and subjected to 10% SDS-PAGE. The protein expression in insect cells can be detected by Coomassie Blue stain ( A, upper panel ), and the proteins were also transferred to polyvinylidene difluoride membrane and detected with CMO2-specific polyclonal antibody ( B, upper panel ). Lower panel, HPLC profile of the cleavage products of all- trans -β-carotene by ferret CMO2 enzyme. All- trans -β-carotene (3 μM) was incubated with the homogenates from either uninfected ( A ) or ferret CMO2 baculovirus-infected ( B ) insect cells for 1 h at 37 °C as described under “Experimental Procedures.” The cleavage products extracted from the incubation mixture were separated by reverse phase HPLC using a C18 column. A peak corresponding to authentic β-apo-10′-carotenal standard ( C ) was detected at 450 nm only in the incubation mixture with the homogenates of ferret CMO2 baculovirus-infected cells ( B ) but not in that of uninfected cells ( A ). D , spectral analysis of the cleavage product ( blue line ) of all- trans -β-carotene versus β -apo-10′-carotenal standard ( red line ). Both the retention time and absorption spectrum of the product from all- trans -β-carotene matched exactly with that of standard β-apo-10′-carotenal.

    Article Snippet: Lycopene, apo-10′-lycopenal, apo-10′-lycopenol, apo-10′-lycopenoic acid, β-carotene, and lycopene 10% dry power (Lycovit® 10%) were provided by BASF Inc, Ludwigshafen, Germany.

    Techniques: Expressing, High Performance Liquid Chromatography, Infection, SDS Page, Staining, Incubation

    Effects of pH, time, protein, and substrate concentrations on β-apo-10′-carotenal productions by ferret CMO2 A, pH optimum of the enzyme was detected at the indicated pH value in the presence of 6 mg of homogenate protein containing ferret CMO2 and 3 μM all- trans -β-carotene at 37 °C for 1 h; B, all- trans -β-carotene (3 μ M) was incubated with 6 mg of homogenate expressing ferret CMO2 at 37 °C for various time points; C, the incubations were carried out using various protein concentrations of cell homogenates expressing ferret CMO2 with 3 μM all- trans -β-carotene at 37° for 1 h; and D, the effect of substrate concentration was conducted with homogenates of insect cells expressing ferret CMO2 with various all- trans -β-carotene concentration. The enzyme reactions were conducted in a volume of 1 ml of assay buffer containing 20 mM Tris-HCl, pH 8.0, 150 mM KCl, 10 μM Fe 2 SO 2 , 3 mM NAD, 0.3 mM DTT, and 6 mg of protein of the insect cell homogenates expressing ferret CMO2 at 37 °C for 60 min, otherwise as indicated on the figures. The procedures for the incubation, extraction, and HPLC analysis were described under “Experimental Procedures.” The data are the average of two independent experiments. The variation in the data between the two experiments was less than 3% of the data average.

    Journal: The Journal of biological chemistry

    Article Title: The Biochemical Characterization of Ferret Carotene-9?, 10?-Monooxygenase Catalyzing Cleavage of Carotenoids in Vitro and in Vivo*

    doi: 10.1074/jbc.M512095200

    Figure Lengend Snippet: Effects of pH, time, protein, and substrate concentrations on β-apo-10′-carotenal productions by ferret CMO2 A, pH optimum of the enzyme was detected at the indicated pH value in the presence of 6 mg of homogenate protein containing ferret CMO2 and 3 μM all- trans -β-carotene at 37 °C for 1 h; B, all- trans -β-carotene (3 μ M) was incubated with 6 mg of homogenate expressing ferret CMO2 at 37 °C for various time points; C, the incubations were carried out using various protein concentrations of cell homogenates expressing ferret CMO2 with 3 μM all- trans -β-carotene at 37° for 1 h; and D, the effect of substrate concentration was conducted with homogenates of insect cells expressing ferret CMO2 with various all- trans -β-carotene concentration. The enzyme reactions were conducted in a volume of 1 ml of assay buffer containing 20 mM Tris-HCl, pH 8.0, 150 mM KCl, 10 μM Fe 2 SO 2 , 3 mM NAD, 0.3 mM DTT, and 6 mg of protein of the insect cell homogenates expressing ferret CMO2 at 37 °C for 60 min, otherwise as indicated on the figures. The procedures for the incubation, extraction, and HPLC analysis were described under “Experimental Procedures.” The data are the average of two independent experiments. The variation in the data between the two experiments was less than 3% of the data average.

    Article Snippet: Lycopene, apo-10′-lycopenal, apo-10′-lycopenol, apo-10′-lycopenoic acid, β-carotene, and lycopene 10% dry power (Lycovit® 10%) were provided by BASF Inc, Ludwigshafen, Germany.

    Techniques: Incubation, Expressing, Concentration Assay, High Performance Liquid Chromatography

    Relative risk (RR) of major cardiovascular disease by eight combinations of all three active antioxidant assignments relative to the all placebo group (right); or of stroke by combinations of active vitamin C and vitamin E assignments relative to the groups with placebo vitamins C and E (left). VC = vitamin C, VE = vitamin E, BC = beta-carotene, CVD = cardiovascular disease.

    Journal: Archives of internal medicine

    Article Title: A Randomized Factorial Trial of Vitamins C, E, and Beta-Carotene in the Secondary Prevention of Cardiovascular Events in Women: Results from the Women’s Antioxidant Cardiovascular Study (WACS)

    doi: 10.1001/archinte.167.15.1610

    Figure Lengend Snippet: Relative risk (RR) of major cardiovascular disease by eight combinations of all three active antioxidant assignments relative to the all placebo group (right); or of stroke by combinations of active vitamin C and vitamin E assignments relative to the groups with placebo vitamins C and E (left). VC = vitamin C, VE = vitamin E, BC = beta-carotene, CVD = cardiovascular disease.

    Article Snippet: WACS is a randomized, double-blind, placebo-controlled trial evaluating the effects of vitamin C (500 mg/day synthetic vitamin C [ascorbic acid], provided by BASF Corporation [Mount Olive, NJ]), vitamin E (600 IU natural vitamin E [D-alpha-tocopherol acetate] every other day, provided by Cognis Corporation [La Grange, IL]), and beta-carotene (50 mg Lurotin every other day, provided by BASF) in the prevention of important vascular events in a 2×2×2 factorial design among high risk women with either a history of vascular disease or at least three cardiovascular risk factors.

    Techniques:

    Densitogram showing peak area of β-carotene. (a) Peak area of β-carotene before 2,2-diphenyl-1-picrylhydrazyl (DPPH) derivatization, (b) peak area of β-carotene after DPPH derivatization

    Journal: Journal of Advanced Pharmaceutical Technology & Research

    Article Title: Antioxidant markers based TLC-DPPH differentiation on four commercialized botanical sources of Shankhpushpi (A Medhya Rasayana): A preliminary assessment

    doi: 10.4103/2231-4040.107497

    Figure Lengend Snippet: Densitogram showing peak area of β-carotene. (a) Peak area of β-carotene before 2,2-diphenyl-1-picrylhydrazyl (DPPH) derivatization, (b) peak area of β-carotene after DPPH derivatization

    Article Snippet: Reagents and Chemicals DPPH, β-carotene, and rutin were obtained from Himedia Laboratories (Mumbai, India).

    Techniques:

    Antioxidant activity of M. cajuput flower and leaf extracts determined by β-carotene bleaching test

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Antioxidant, antibacterial activity, and phytochemical characterization of Melaleuca cajuputi extract

    doi: 10.1186/s12906-015-0914-y

    Figure Lengend Snippet: Antioxidant activity of M. cajuput flower and leaf extracts determined by β-carotene bleaching test

    Article Snippet: Chemical and reagents Methanol, sodium hydroxide (NaOH), iron (II) sulfate (FeSO4 ), iron (II) chloride (FeCl2 ), sodium nitrite, iron (II) chloride (FeCl3 ), chloroform, hydrochloric acid (HCL), β-carotene, quercetin, chloragenic acid, tripyridyl-s-triazine (TPTZ), butylated hydroxytoluene (BHT), propyl gallate, 2,2-diphenyl −1- picrylhydrazyl (DPPH), linoleic acid, Tween 20, Folin-ciocalteu reagent, acetate buffer, ferrozine, ethylenediaminetetraacetic acid (EDTA), ascorbic acid, and all other reagents and solvents used in this study were of analytical grade purchased from Merck (Merck, Darmstadt, Germany).

    Techniques: Antioxidant Activity Assay

    Response surface model plot showing the effects of independent variables on β-carotene content: panel A temperature and power; panel B temperature and time; and panel C power and time.

    Journal: Molecules

    Article Title: Employing Response Surface Methodology for the Optimization of Ultrasound Assisted Extraction of Lutein and β-Carotene from Spinach

    doi: 10.3390/molecules20046611

    Figure Lengend Snippet: Response surface model plot showing the effects of independent variables on β-carotene content: panel A temperature and power; panel B temperature and time; and panel C power and time.

    Article Snippet: Lutein (purity 95%) was procured from Indofine Chemical Company (Hillsborough Township, NJ, USA) and β-carotene (purity 98.1%) was purchased from MP Biomedicals LLC (Santa Ana, CA, USA).

    Techniques:

    The chromatographic separation of lutein (L) and β-carotene (B): ( A ) real visible light image; ( B ) UV image at 254 nm; and ( C ) grey scale image by Quantity One software. Spots: 1 to 17 for spinach extracts; L1 and L2 for lutein controls; and B1 and B2 for β-carotene controls.

    Journal: Molecules

    Article Title: Employing Response Surface Methodology for the Optimization of Ultrasound Assisted Extraction of Lutein and β-Carotene from Spinach

    doi: 10.3390/molecules20046611

    Figure Lengend Snippet: The chromatographic separation of lutein (L) and β-carotene (B): ( A ) real visible light image; ( B ) UV image at 254 nm; and ( C ) grey scale image by Quantity One software. Spots: 1 to 17 for spinach extracts; L1 and L2 for lutein controls; and B1 and B2 for β-carotene controls.

    Article Snippet: Lutein (purity 95%) was procured from Indofine Chemical Company (Hillsborough Township, NJ, USA) and β-carotene (purity 98.1%) was purchased from MP Biomedicals LLC (Santa Ana, CA, USA).

    Techniques: Software

    Mass spectra of spinach extract TLC spot LS of experimental condition (panel A ) and TLC spot BS of experimental condition (panel C ) excised and compared to lutein (panel B ) and β-carotene (panel D ) standards.

    Journal: Molecules

    Article Title: Employing Response Surface Methodology for the Optimization of Ultrasound Assisted Extraction of Lutein and β-Carotene from Spinach

    doi: 10.3390/molecules20046611

    Figure Lengend Snippet: Mass spectra of spinach extract TLC spot LS of experimental condition (panel A ) and TLC spot BS of experimental condition (panel C ) excised and compared to lutein (panel B ) and β-carotene (panel D ) standards.

    Article Snippet: Lutein (purity 95%) was procured from Indofine Chemical Company (Hillsborough Township, NJ, USA) and β-carotene (purity 98.1%) was purchased from MP Biomedicals LLC (Santa Ana, CA, USA).

    Techniques: Thin Layer Chromatography

    Analysis of NinaB’s enzymatic action on 20, 20’-di-nor-β-carotene. (A) HPLC profiles at 360 nm of lipid extracts from in vitro tests for enzymatic activity from NinaB cell lysate (dashed trace) and control (solid trace). (B) Schematic depicting of the catalytic action on 20, 20’-di-nor-β-carotene by NinaB. (C) UV/Vis spectra of the corresponding products and the substrate in (A).

    Journal: ACS chemical biology

    Article Title: The biochemical basis of vitamin A3 production in arthropod vision

    doi: 10.1021/acschembio.5b00967

    Figure Lengend Snippet: Analysis of NinaB’s enzymatic action on 20, 20’-di-nor-β-carotene. (A) HPLC profiles at 360 nm of lipid extracts from in vitro tests for enzymatic activity from NinaB cell lysate (dashed trace) and control (solid trace). (B) Schematic depicting of the catalytic action on 20, 20’-di-nor-β-carotene by NinaB. (C) UV/Vis spectra of the corresponding products and the substrate in (A).

    Article Snippet: All- trans -3’-dehydrolutein, 3-hydroxy-β-apo-8’-carotenal and 20, 20-di- nor -β-carotene were generous gifts from DSM Nutritional Products and were synthesized respectively according to – .

    Techniques: High Performance Liquid Chromatography, In Vitro, Activity Assay

    Purification and tests for enzymatic activity of NinaB on β,β-carotene. (A) Coomasie stained SDS-PAGE gel showing purity of recombinant NinaB after purification with Talon Co 2+ resin. (B) HPLC profiles at 360 nm of lipid extracts from in vitro tests for enzymatic activity from NinaB cell lysate (blue trace) and Talon purified NinaB enzyme (red trace). (C) UV/Vis spectra of corresponding peaks in (B) peak. a, β,β-carotene; b, 11- cis -retinal; c, all- trans -retinal.

    Journal: ACS chemical biology

    Article Title: The biochemical basis of vitamin A3 production in arthropod vision

    doi: 10.1021/acschembio.5b00967

    Figure Lengend Snippet: Purification and tests for enzymatic activity of NinaB on β,β-carotene. (A) Coomasie stained SDS-PAGE gel showing purity of recombinant NinaB after purification with Talon Co 2+ resin. (B) HPLC profiles at 360 nm of lipid extracts from in vitro tests for enzymatic activity from NinaB cell lysate (blue trace) and Talon purified NinaB enzyme (red trace). (C) UV/Vis spectra of corresponding peaks in (B) peak. a, β,β-carotene; b, 11- cis -retinal; c, all- trans -retinal.

    Article Snippet: All- trans -3’-dehydrolutein, 3-hydroxy-β-apo-8’-carotenal and 20, 20-di- nor -β-carotene were generous gifts from DSM Nutritional Products and were synthesized respectively according to – .

    Techniques: Purification, Activity Assay, Staining, SDS Page, Recombinant, High Performance Liquid Chromatography, In Vitro

    Effect (mean±S.E.) of Cry1Ac and carotenoids on CAT, SOD, GST activity on Ostrinia nubilalis larvae. Enzyme activity in the fifth stage larvae fed on a) diets made with grain of different maize lines (4Bt, HC, M37W, and 4BtxHC) b) diets made with different amounts of added β-carotene. In (a) the treatments were M37W, HC, 4Bt and 4BtxHC diets, while in (b), different quantities of β-carotene (0.6, 6, and 60 mg/100 mg of diet) were added to 4Bt and M37W diets (4Bt, 4Bt+0.6, 4Bt+6, 4Bt+60, M37W, M37W+6). Fifth stage larvae were used and results are shown two days after exposure. CAT: Catalase; SOD: Superoxide dismutase; GST: Glutathione S-transferase. Different letters indicate statistically significant differences between diets (P

    Journal: PLoS ONE

    Article Title: Carotenoids moderate the effectiveness of a Bt gene against the European corn borer, Ostrinia nubilalis

    doi: 10.1371/journal.pone.0199317

    Figure Lengend Snippet: Effect (mean±S.E.) of Cry1Ac and carotenoids on CAT, SOD, GST activity on Ostrinia nubilalis larvae. Enzyme activity in the fifth stage larvae fed on a) diets made with grain of different maize lines (4Bt, HC, M37W, and 4BtxHC) b) diets made with different amounts of added β-carotene. In (a) the treatments were M37W, HC, 4Bt and 4BtxHC diets, while in (b), different quantities of β-carotene (0.6, 6, and 60 mg/100 mg of diet) were added to 4Bt and M37W diets (4Bt, 4Bt+0.6, 4Bt+6, 4Bt+60, M37W, M37W+6). Fifth stage larvae were used and results are shown two days after exposure. CAT: Catalase; SOD: Superoxide dismutase; GST: Glutathione S-transferase. Different letters indicate statistically significant differences between diets (P

    Article Snippet: Mortality in larvae fed on a diet containing kernels of 4Bt plus different quantities (0.6, 6.0, and 60 mg/100 g of diet, referred to as 4Bt+0.6, 4Bt+6, and 4Bt+60, respectively) of β-carotene (TCI America-TCI Chemicals, CAS: 7235-40-7) was recorded weekly from L5 to pupation, until week 3 when all larvae fed the non-Bt diet had pupated.

    Techniques: Activity Assay

    Changes in mortality percentage (mean±S.E.) of Ostrinia nubilalis larvae fed on diets made with kernels of different maize lines when different quantities of β-carotene were added to the diet (in mg/100g diet). 4Bt contained the Cry1Ac toxin, M37W was the near isogenic line, and 4BtxHC was the line with the two traits. Fifth stage larvae were treated and mortality was recorded every week for three weeks. Week 1: [F = 2.68; P

    Journal: PLoS ONE

    Article Title: Carotenoids moderate the effectiveness of a Bt gene against the European corn borer, Ostrinia nubilalis

    doi: 10.1371/journal.pone.0199317

    Figure Lengend Snippet: Changes in mortality percentage (mean±S.E.) of Ostrinia nubilalis larvae fed on diets made with kernels of different maize lines when different quantities of β-carotene were added to the diet (in mg/100g diet). 4Bt contained the Cry1Ac toxin, M37W was the near isogenic line, and 4BtxHC was the line with the two traits. Fifth stage larvae were treated and mortality was recorded every week for three weeks. Week 1: [F = 2.68; P

    Article Snippet: Mortality in larvae fed on a diet containing kernels of 4Bt plus different quantities (0.6, 6.0, and 60 mg/100 g of diet, referred to as 4Bt+0.6, 4Bt+6, and 4Bt+60, respectively) of β-carotene (TCI America-TCI Chemicals, CAS: 7235-40-7) was recorded weekly from L5 to pupation, until week 3 when all larvae fed the non-Bt diet had pupated.

    Techniques:

    ( a ) Time dependence of ESA (λ det = 550 nm) of β-carotene in octane (squares) observed after TPE (λ exc = 1,310 nm). Monoexponential fit: 9 ± 0.2 ps (solid line). ( b ) Power dependence of ESA. Power fit: Exponent = 2.2 ± 0.3 (solid line). ( c ) Relative intensity of ESA excited with linear and circular polarized light (Ω = 0.84 ± 0.07). The “coherence spike” is very large in the two-photon experiments (off scale) and has a quadratic dependence on the excitation power and a linear dependence on the Car concentration. No spike was seen with a pure buffer sample. In experiments with chlorophyll a , which has no two-photon allowed states, the coherence spike was found to be substantially smaller in magnitude (data not shown).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Femtosecond dynamics of the forbidden carotenoid S1 state in light-harvesting complexes of purple bacteria observed after two-photon excitation

    doi:

    Figure Lengend Snippet: ( a ) Time dependence of ESA (λ det = 550 nm) of β-carotene in octane (squares) observed after TPE (λ exc = 1,310 nm). Monoexponential fit: 9 ± 0.2 ps (solid line). ( b ) Power dependence of ESA. Power fit: Exponent = 2.2 ± 0.3 (solid line). ( c ) Relative intensity of ESA excited with linear and circular polarized light (Ω = 0.84 ± 0.07). The “coherence spike” is very large in the two-photon experiments (off scale) and has a quadratic dependence on the excitation power and a linear dependence on the Car concentration. No spike was seen with a pure buffer sample. In experiments with chlorophyll a , which has no two-photon allowed states, the coherence spike was found to be substantially smaller in magnitude (data not shown).

    Article Snippet: LH2s of Rps. acidophila were isolated from Rps. acidophila strain 7050 by using the methods described in refs. and . β-Carotene (Acros, New Jersey) was dissolved in octane by sonicating for ≈10 s and centrifuging for 4 min at ≈8,000 g. The solutions had an optical density of ≈1 per mm at the maximum of the absorption.

    Techniques: Concentration Assay