β-actin Millipore Search Results


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  • 99
    Millipore anti β actin millipore antibody
    Anti β Actin Millipore Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore millipore β actin
    Millipore β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore inhibin βa
    Expression of TGFβ factors in the OFT. Immunohistochemistry of <t>Inhibin</t> <t>βA</t> (A) and p-Smad3 (B) in the hearts of normal E10.5 embryos. Broken lines mark the boundaries between the endocardial cushion (ecc) and myocardium (myc). Scale bar
    Inhibin βa, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc anti β actin
    Expression of TGFβ factors in the OFT. Immunohistochemistry of <t>Inhibin</t> <t>βA</t> (A) and p-Smad3 (B) in the hearts of normal E10.5 embryos. Broken lines mark the boundaries between the endocardial cushion (ecc) and myocardium (myc). Scale bar
    Anti β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 9886 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore β actin
    Sulfasalazine decreases tamoxifen-induced autophagic cell death in human RPE cells. LC3B I and II expression in the primary H-RPE cells (A, n = 3) and ARPE-19 cells (B, n = 3) was assessed by western blotting at various points after vehicle, sulfasalazine, tamoxifen,or tamoxifen plus sulfasalazine administration. Also, <t>β-actin</t> was used as a control for normalization. This blot is representative of the three independent experiments. Also, *P
    β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 67298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA β actin
    Effect of D-pinitol on renal expression of SODs in chronic CsA nephropathy. (A) Representative western blot showing the effect of D-pinitol on the expression of SOD1 and SOD2 in chronic CsA nephropathy. (B) Quantitative analyses for <t>SOD1/β-actin.</t> * P=0.050 vs. CsA+pinitol group. (C) Quantitative analyses for SOD2/β-actin. Cont, vehicle-treated control group; Pinitol, D-pinitol; CsA, cyclosporine A; SOD, superoxide dismutase.
    β Actin, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore α β actin
    Itk signaling controls the expression of both T-bet and IFN-γ to regulate IL-4 secretion in human peripheral blood T cells. A, Human peripheral blood T cells transduced with either control lentivirus (shNull) or carrying shRNA against Itk (shItk) were stimulated with α-CD3/CD28 then analyzed for Itk mRNA (left) or protein levels from total cell lysates probed with α-Itk (top right) or <t>α-β-actin</t> (bottom right) . B, Cells treated as in Fig 3, A , were restimulated for 24 hours and analyzed for T-bet and IFN-γ expression by using qRT-PCR. Values in Fig 3, A and B , are means ± SEMs of at least 4 individual donors. * P
    α β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore β‑actin
    Itk signaling controls the expression of both T-bet and IFN-γ to regulate IL-4 secretion in human peripheral blood T cells. A, Human peripheral blood T cells transduced with either control lentivirus (shNull) or carrying shRNA against Itk (shItk) were stimulated with α-CD3/CD28 then analyzed for Itk mRNA (left) or protein levels from total cell lysates probed with α-Itk (top right) or <t>α-β-actin</t> (bottom right) . B, Cells treated as in Fig 3, A , were restimulated for 24 hours and analyzed for T-bet and IFN-γ expression by using qRT-PCR. Values in Fig 3, A and B , are means ± SEMs of at least 4 individual donors. * P
    β‑Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore β actin hrp
    Itk signaling controls the expression of both T-bet and IFN-γ to regulate IL-4 secretion in human peripheral blood T cells. A, Human peripheral blood T cells transduced with either control lentivirus (shNull) or carrying shRNA against Itk (shItk) were stimulated with α-CD3/CD28 then analyzed for Itk mRNA (left) or protein levels from total cell lysates probed with α-Itk (top right) or <t>α-β-actin</t> (bottom right) . B, Cells treated as in Fig 3, A , were restimulated for 24 hours and analyzed for T-bet and IFN-γ expression by using qRT-PCR. Values in Fig 3, A and B , are means ± SEMs of at least 4 individual donors. * P
    β Actin Hrp, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore β actin primers
    Effect of silibinin treatment on the gene expression of NER regulators and GADD45α in UVB-exposed NHDFs NHDFs were plated and divided into two groups: pre and post-UVB treatment. In the pre-UVB treatment group, cells were treated with silibinin and/or pifithrin for 3 hrs, then washed and exposed to UVB 1 mJ/cm 2 . Treatment with silibinin or pifithrin alone served as relevant controls. In the post-UVB group, cells were exposed first to UVB 1 mJ/cm 2 and then immediately treated with silibinin and/or pifithrin for 3 hrs. At the end of the described treatments, the mRNA levels of A. XPA, B. XPB, C. XPC, D. XPG and E. GADD45α were measured by real-time RT-PCR as described in methods. Samples were run in triplicate, and three independent experiments were performed for every gene of interest. The mRNA level of gene was normalized to those of <t>β-actin</t> mRNA in each sample. *; p
    β Actin Primers, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore protein β actin
    AXL-APTAMER Inhibits Downstream Signaling in OC Cells (A) pAXL levels after AXL-APTAMER treatment detected by western blot alaysis. SKOV3-IP OC cells were treated for 3 hr with a control aptamer or AXL-APTAMER. Cell lysates were immunoprecipitated with pAXL. <t>β-actin</t> was used as the housekeeping protein. Quantification of the bands was performed using ImageJ software. (B) Cell viability was detected by MTS assay for SKOV3-IP1 and HeyA8 cell line using three different time points (24, 48, and 72 hr). All experiments were performed in six replicates, and the results were reported as the mean absorption ± SEM (**p
    Protein β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck KGaA anti β actin
    (a) Western blotting for EGFR (170 kDa) and Beclin1 (60 kDa) in representative H-EGFR/L-Beclin1 (lanes 1 and 2), L-EGFR/H-Beclin1 (lanes 3–5), H-EGFR/H-Beclin1 (lanes 6–8), and L-EGFR/L-Beclin1 (lanes 9–11) GBs. (b) Up- or downregulation of either EGFR or Beclin1 is quantified by densitometric data analysis, which shows the relative expression of EGFR and Beclin1 after normalization to the  β -actin bands. Data are reported as means ± S.E. of three densitometric analyses of the same sample.
    Anti β Actin, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore β actin immunoblots
    (a) Western blotting for EGFR (170 kDa) and Beclin1 (60 kDa) in representative H-EGFR/L-Beclin1 (lanes 1 and 2), L-EGFR/H-Beclin1 (lanes 3–5), H-EGFR/H-Beclin1 (lanes 6–8), and L-EGFR/L-Beclin1 (lanes 9–11) GBs. (b) Up- or downregulation of either EGFR or Beclin1 is quantified by densitometric data analysis, which shows the relative expression of EGFR and Beclin1 after normalization to the  β -actin bands. Data are reported as means ± S.E. of three densitometric analyses of the same sample.
    β Actin Immunoblots, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore bax6a7and β actin
    (a) Western blotting for EGFR (170 kDa) and Beclin1 (60 kDa) in representative H-EGFR/L-Beclin1 (lanes 1 and 2), L-EGFR/H-Beclin1 (lanes 3–5), H-EGFR/H-Beclin1 (lanes 6–8), and L-EGFR/L-Beclin1 (lanes 9–11) GBs. (b) Up- or downregulation of either EGFR or Beclin1 is quantified by densitometric data analysis, which shows the relative expression of EGFR and Beclin1 after normalization to the  β -actin bands. Data are reported as means ± S.E. of three densitometric analyses of the same sample.
    Bax6a7and β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore β actin detection
    Stability of mutant core proteins. Western blot analysis of lysates harvested at 48 h postelectroporation. Results for core (top panels), NS5A (middle panels), and <t>β-actin</t> (lower panels) are shown. Residues mutated to alanine are identified. The
    β Actin Detection, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore murine β actin
    Stability of mutant core proteins. Western blot analysis of lysates harvested at 48 h postelectroporation. Results for core (top panels), NS5A (middle panels), and <t>β-actin</t> (lower panels) are shown. Residues mutated to alanine are identified. The
    Murine β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore cytoplasmic β actin
    Increased ACF mRNA and protein expression in Ob/Ob liver. A . The abundance of Ob/Ob hepatic apoB, apobec-1 and acf mRNA relative to that quantified from lean control liver was assessed by real time PCR and normalized to <t>β-actin</t> mRNA. Lean animal
    Cytoplasmic β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β actin antibodiy
    Increased ACF mRNA and protein expression in Ob/Ob liver. A . The abundance of Ob/Ob hepatic apoB, apobec-1 and acf mRNA relative to that quantified from lean control liver was assessed by real time PCR and normalized to <t>β-actin</t> mRNA. Lean animal
    β Actin Antibodiy, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore β actin protein
    mRNA expression in callus tissue of control and PDLLA treated rats 2, 4 and 6 weeks post-surgery. Expression level of (A) Runx2, (B) HIF2A, (C) Wnt4, (D) Wnt10b, (E) GSK-3β, (F) β-catenin and (G) TCF-1. <t>Β-actin</t> is a control. Values are presented as mean ± standard deviation, *P
    β Actin Protein, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 374 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Expression of TGFβ factors in the OFT. Immunohistochemistry of Inhibin βA (A) and p-Smad3 (B) in the hearts of normal E10.5 embryos. Broken lines mark the boundaries between the endocardial cushion (ecc) and myocardium (myc). Scale bar

    Journal: Birth defects research. Part B, Developmental and reproductive toxicology

    Article Title: TGFβ and Wnt in Cardiac Outflow Tract Defects in Offspring of Diabetic Pregnancies

    doi: 10.1002/bdrb.21120

    Figure Lengend Snippet: Expression of TGFβ factors in the OFT. Immunohistochemistry of Inhibin βA (A) and p-Smad3 (B) in the hearts of normal E10.5 embryos. Broken lines mark the boundaries between the endocardial cushion (ecc) and myocardium (myc). Scale bar

    Article Snippet: For immunohistochemistry, the sections were boiled in a citrate buffer (10 mM Citrate Acid, pH 6.0) for 5 minutes to unmask antigens, equilibrated in PBS (pH 7.4), incubated with primary antibodies recognizing 4-hydroxynonenal (4-HNE; Millipore, Temecula, CA), 3-nitrotyrosine (3-NT, Cell Signaling Technology, Danvers, MA), protein disulfide isomerase (PDI; Cell Signaling Technology), phosphorylated (p-) calnexin (Abcam, Cambridge, MA), Inhibin βA (EMD Biosciences, Gibbstown, NJ), and p-Smad3 (Cell Signaling Technology) ( ).

    Techniques: Expressing, Immunohistochemistry

    Sulfasalazine decreases tamoxifen-induced autophagic cell death in human RPE cells. LC3B I and II expression in the primary H-RPE cells (A, n = 3) and ARPE-19 cells (B, n = 3) was assessed by western blotting at various points after vehicle, sulfasalazine, tamoxifen,or tamoxifen plus sulfasalazine administration. Also, β-actin was used as a control for normalization. This blot is representative of the three independent experiments. Also, *P

    Journal: BMB Reports

    Article Title: Sulfasalazine attenuates tamoxifen-induced toxicity in human retinal pigment epithelial cells

    doi: 10.5483/BMBRep.2020.53.5.041

    Figure Lengend Snippet: Sulfasalazine decreases tamoxifen-induced autophagic cell death in human RPE cells. LC3B I and II expression in the primary H-RPE cells (A, n = 3) and ARPE-19 cells (B, n = 3) was assessed by western blotting at various points after vehicle, sulfasalazine, tamoxifen,or tamoxifen plus sulfasalazine administration. Also, β-actin was used as a control for normalization. This blot is representative of the three independent experiments. Also, *P

    Article Snippet: Caspase-3 and cleaved caspase-3 were obtained from Cell Signaling Technology (Danvers, MA), whereas the LC3B and β-actin were obtained from Sigma-Aldrich.

    Techniques: Expressing, Western Blot

    Effect of inflammation on NaPi-IIb expression in TNBS colitis rats. Three-week-old rats were treated with TNBS (1 mg/rat) and were harvested 6 days after TNBS administration. A : BBMVs were isolated from the jejunal mucosa of rats treated with PBS or TNBS and used for Western detection. A 1:4,000 dilution of the mouse NaPi-IIb antibody was used. The expression of NaPi-IIb protein is calculated by the density of NaPi-IIb band over that of β-actin band. Bar chart shows NaPi-IIb protein expression indicated as means ± SE in the sum of 3 independent experiments. * P ≤ 0.04 for control groups vs. TNBS groups. Inset : a typical immunoblot image. B : RNAs were isolated from the jejunal mucosa of control or TNBS rats and used for real-time PCR. NaPi-IIb mRNA and TATA binding protein (TBP) mRNA were amplified with rat-specific NaPi-IIb and TBP primers. The changes in NaPi-IIb gene expression are analyzed by the comparative cycle threshold (Ct) method. Data are means ± SE from total 18 rats (9 for TNBS group, 9 for control group). * P ≤ 0.004 for control group vs. TNBS group.

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Tumor necrosis factor-alpha impairs intestinal phosphate absorption in colitis

    doi: 10.1152/ajpgi.90722.2008

    Figure Lengend Snippet: Effect of inflammation on NaPi-IIb expression in TNBS colitis rats. Three-week-old rats were treated with TNBS (1 mg/rat) and were harvested 6 days after TNBS administration. A : BBMVs were isolated from the jejunal mucosa of rats treated with PBS or TNBS and used for Western detection. A 1:4,000 dilution of the mouse NaPi-IIb antibody was used. The expression of NaPi-IIb protein is calculated by the density of NaPi-IIb band over that of β-actin band. Bar chart shows NaPi-IIb protein expression indicated as means ± SE in the sum of 3 independent experiments. * P ≤ 0.04 for control groups vs. TNBS groups. Inset : a typical immunoblot image. B : RNAs were isolated from the jejunal mucosa of control or TNBS rats and used for real-time PCR. NaPi-IIb mRNA and TATA binding protein (TBP) mRNA were amplified with rat-specific NaPi-IIb and TBP primers. The changes in NaPi-IIb gene expression are analyzed by the comparative cycle threshold (Ct) method. Data are means ± SE from total 18 rats (9 for TNBS group, 9 for control group). * P ≤ 0.004 for control group vs. TNBS group.

    Article Snippet: A 1:5,000 dilution of the β-actin antiserum (Sigma Aldrich, St. Louis, MO) was used to detect β-actin protein.

    Techniques: Expressing, Isolation, Western Blot, Real-time Polymerase Chain Reaction, Binding Assay, Amplification

    Effect of inflammation on intestinal phosphate absorption and type IIb sodium-phosphate cotransporter (NaPi-IIb) expression in trinitrobenzene sulfonic acid (TNBS) colitis mice. Six-week-old male mice were treated with TNBS (2 mg/mouse) and were harvested 6 days after TNBS administration. A : brush-border membrane (BBM) phosphate uptake in TNBS colitis mice. BBM vesicles (BBMVs) were isolated from the ileal mucosa of mice treated with PBS or TNBS, and BBM phosphate uptake was performed. The contribution of sodium-dependent uptake was calculated by subtracting the sodium-independent uptake values observed in the absence of sodium from the uptake values in the presence of sodium. Data presented are means ± SE in 3 or 4 different groups of animals. * P ≤ 0.028 for TNBS mice vs. control mice. B : NaPi-IIb expression in TNBS colitis mice. BBMVs were isolated from the ileal mucosa of mice treated with PBS or TNBS and used for Western detection. A 1:4,000 dilution of the mouse NaPi-IIb antibody was used. The expression of NaPi-IIb protein is calculated by the density of NaPi-IIb band over that of β-actin band. Bar chart shows NaPi-IIb protein expression indicated as means ± SE in the sum of 3 independent experiments. * P ≤ 0.017 for control groups vs. TNBS groups. Inset : typical immunoblot image.

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Tumor necrosis factor-alpha impairs intestinal phosphate absorption in colitis

    doi: 10.1152/ajpgi.90722.2008

    Figure Lengend Snippet: Effect of inflammation on intestinal phosphate absorption and type IIb sodium-phosphate cotransporter (NaPi-IIb) expression in trinitrobenzene sulfonic acid (TNBS) colitis mice. Six-week-old male mice were treated with TNBS (2 mg/mouse) and were harvested 6 days after TNBS administration. A : brush-border membrane (BBM) phosphate uptake in TNBS colitis mice. BBM vesicles (BBMVs) were isolated from the ileal mucosa of mice treated with PBS or TNBS, and BBM phosphate uptake was performed. The contribution of sodium-dependent uptake was calculated by subtracting the sodium-independent uptake values observed in the absence of sodium from the uptake values in the presence of sodium. Data presented are means ± SE in 3 or 4 different groups of animals. * P ≤ 0.028 for TNBS mice vs. control mice. B : NaPi-IIb expression in TNBS colitis mice. BBMVs were isolated from the ileal mucosa of mice treated with PBS or TNBS and used for Western detection. A 1:4,000 dilution of the mouse NaPi-IIb antibody was used. The expression of NaPi-IIb protein is calculated by the density of NaPi-IIb band over that of β-actin band. Bar chart shows NaPi-IIb protein expression indicated as means ± SE in the sum of 3 independent experiments. * P ≤ 0.017 for control groups vs. TNBS groups. Inset : typical immunoblot image.

    Article Snippet: A 1:5,000 dilution of the β-actin antiserum (Sigma Aldrich, St. Louis, MO) was used to detect β-actin protein.

    Techniques: Expressing, Mouse Assay, Isolation, Western Blot

    Effect of D-pinitol on renal expression of SODs in chronic CsA nephropathy. (A) Representative western blot showing the effect of D-pinitol on the expression of SOD1 and SOD2 in chronic CsA nephropathy. (B) Quantitative analyses for SOD1/β-actin. * P=0.050 vs. CsA+pinitol group. (C) Quantitative analyses for SOD2/β-actin. Cont, vehicle-treated control group; Pinitol, D-pinitol; CsA, cyclosporine A; SOD, superoxide dismutase.

    Journal: International Journal of Molecular Medicine

    Article Title: D-Pinitol alleviates cyclosporine A-induced renal tubulointerstitial fibrosis via activating Sirt1 and Nrf2 antioxidant pathways

    doi: 10.3892/ijmm.2018.3408

    Figure Lengend Snippet: Effect of D-pinitol on renal expression of SODs in chronic CsA nephropathy. (A) Representative western blot showing the effect of D-pinitol on the expression of SOD1 and SOD2 in chronic CsA nephropathy. (B) Quantitative analyses for SOD1/β-actin. * P=0.050 vs. CsA+pinitol group. (C) Quantitative analyses for SOD2/β-actin. Cont, vehicle-treated control group; Pinitol, D-pinitol; CsA, cyclosporine A; SOD, superoxide dismutase.

    Article Snippet: Antibodies against superoxide dismutase SOD1 (SOD1; 1:10,000; cat. no. ADI-SOD-100; Enzo Life Sciences, Inc., Farmingdale, NY, USA), SOD2 (dilution, 1:10,000; cat. no. ab16956), catalase (dilution, 1:2,000; cat. no. ab31630), FoxO1 (dilution, 1:1,000; cat. no. ab39670), phospho-FoxO1A S329 (dilution, 1:1,000; cat. no. ab58519) (all from Abcam), heme oxygenase-1 (HO-1; dilution, 1:1,000; cat. no. PA5-27338; BD Biosciences, Franklin Lakes, NJ, USA), and β-actin (dilution, 1:10,000; cat. no. 061M4808; Merck Millipore) were also commercially obtained.

    Techniques: Expressing, Western Blot

    Effect of D-pinitol on renal expression of Sirt1 in chronic CsA nephropathy. (A) Representative western blot showing the effect of D-pinitol on the expression of Sirt1 in chronic CsA nephropathy. (B) Quantitative analyses for Sirt1/β-actin. * P

    Journal: International Journal of Molecular Medicine

    Article Title: D-Pinitol alleviates cyclosporine A-induced renal tubulointerstitial fibrosis via activating Sirt1 and Nrf2 antioxidant pathways

    doi: 10.3892/ijmm.2018.3408

    Figure Lengend Snippet: Effect of D-pinitol on renal expression of Sirt1 in chronic CsA nephropathy. (A) Representative western blot showing the effect of D-pinitol on the expression of Sirt1 in chronic CsA nephropathy. (B) Quantitative analyses for Sirt1/β-actin. * P

    Article Snippet: Antibodies against superoxide dismutase SOD1 (SOD1; 1:10,000; cat. no. ADI-SOD-100; Enzo Life Sciences, Inc., Farmingdale, NY, USA), SOD2 (dilution, 1:10,000; cat. no. ab16956), catalase (dilution, 1:2,000; cat. no. ab31630), FoxO1 (dilution, 1:1,000; cat. no. ab39670), phospho-FoxO1A S329 (dilution, 1:1,000; cat. no. ab58519) (all from Abcam), heme oxygenase-1 (HO-1; dilution, 1:1,000; cat. no. PA5-27338; BD Biosciences, Franklin Lakes, NJ, USA), and β-actin (dilution, 1:10,000; cat. no. 061M4808; Merck Millipore) were also commercially obtained.

    Techniques: Expressing, Western Blot

    Effect of D-pinitol on renal expression of Nrf2, Keap 1 and nuclear Nrf2. (A) Representative western blot showing the effect of D-pinitol on the expression of Nrf2, Keap1 and nuclear Nrf2 in chronic CsA nephropathy. (B) Quantitative analyses of results for total Nrf2/β-actin. * P=0.001 vs. Cont group; ** P

    Journal: International Journal of Molecular Medicine

    Article Title: D-Pinitol alleviates cyclosporine A-induced renal tubulointerstitial fibrosis via activating Sirt1 and Nrf2 antioxidant pathways

    doi: 10.3892/ijmm.2018.3408

    Figure Lengend Snippet: Effect of D-pinitol on renal expression of Nrf2, Keap 1 and nuclear Nrf2. (A) Representative western blot showing the effect of D-pinitol on the expression of Nrf2, Keap1 and nuclear Nrf2 in chronic CsA nephropathy. (B) Quantitative analyses of results for total Nrf2/β-actin. * P=0.001 vs. Cont group; ** P

    Article Snippet: Antibodies against superoxide dismutase SOD1 (SOD1; 1:10,000; cat. no. ADI-SOD-100; Enzo Life Sciences, Inc., Farmingdale, NY, USA), SOD2 (dilution, 1:10,000; cat. no. ab16956), catalase (dilution, 1:2,000; cat. no. ab31630), FoxO1 (dilution, 1:1,000; cat. no. ab39670), phospho-FoxO1A S329 (dilution, 1:1,000; cat. no. ab58519) (all from Abcam), heme oxygenase-1 (HO-1; dilution, 1:1,000; cat. no. PA5-27338; BD Biosciences, Franklin Lakes, NJ, USA), and β-actin (dilution, 1:10,000; cat. no. 061M4808; Merck Millipore) were also commercially obtained.

    Techniques: Expressing, Western Blot

    Effect of D-pinitol on renal expression of HO-1, NQO1 and catalase. (A) Representative western blot showing the effect of D-pinitol on the expression of HO-1, NQO1 and catalase in chronic CsA nephropathy. (B) Quantitative analyses of results for HO-1/β-actin. * P=0.002 vs. CsA group. (C) Quantitative analyses of results for NQO1/β-actin. * P

    Journal: International Journal of Molecular Medicine

    Article Title: D-Pinitol alleviates cyclosporine A-induced renal tubulointerstitial fibrosis via activating Sirt1 and Nrf2 antioxidant pathways

    doi: 10.3892/ijmm.2018.3408

    Figure Lengend Snippet: Effect of D-pinitol on renal expression of HO-1, NQO1 and catalase. (A) Representative western blot showing the effect of D-pinitol on the expression of HO-1, NQO1 and catalase in chronic CsA nephropathy. (B) Quantitative analyses of results for HO-1/β-actin. * P=0.002 vs. CsA group. (C) Quantitative analyses of results for NQO1/β-actin. * P

    Article Snippet: Antibodies against superoxide dismutase SOD1 (SOD1; 1:10,000; cat. no. ADI-SOD-100; Enzo Life Sciences, Inc., Farmingdale, NY, USA), SOD2 (dilution, 1:10,000; cat. no. ab16956), catalase (dilution, 1:2,000; cat. no. ab31630), FoxO1 (dilution, 1:1,000; cat. no. ab39670), phospho-FoxO1A S329 (dilution, 1:1,000; cat. no. ab58519) (all from Abcam), heme oxygenase-1 (HO-1; dilution, 1:1,000; cat. no. PA5-27338; BD Biosciences, Franklin Lakes, NJ, USA), and β-actin (dilution, 1:10,000; cat. no. 061M4808; Merck Millipore) were also commercially obtained.

    Techniques: Expressing, Western Blot

    Itk signaling controls the expression of both T-bet and IFN-γ to regulate IL-4 secretion in human peripheral blood T cells. A, Human peripheral blood T cells transduced with either control lentivirus (shNull) or carrying shRNA against Itk (shItk) were stimulated with α-CD3/CD28 then analyzed for Itk mRNA (left) or protein levels from total cell lysates probed with α-Itk (top right) or α-β-actin (bottom right) . B, Cells treated as in Fig 3, A , were restimulated for 24 hours and analyzed for T-bet and IFN-γ expression by using qRT-PCR. Values in Fig 3, A and B , are means ± SEMs of at least 4 individual donors. * P

    Journal: The Journal of allergy and clinical immunology

    Article Title: IL-2–inducible T-cell kinase modulates TH2-mediated allergic airway inflammation by suppressing IFN-γ in naive CD4+ T cells

    doi: 10.1016/j.jaci.2013.04.033

    Figure Lengend Snippet: Itk signaling controls the expression of both T-bet and IFN-γ to regulate IL-4 secretion in human peripheral blood T cells. A, Human peripheral blood T cells transduced with either control lentivirus (shNull) or carrying shRNA against Itk (shItk) were stimulated with α-CD3/CD28 then analyzed for Itk mRNA (left) or protein levels from total cell lysates probed with α-Itk (top right) or α-β-actin (bottom right) . B, Cells treated as in Fig 3, A , were restimulated for 24 hours and analyzed for T-bet and IFN-γ expression by using qRT-PCR. Values in Fig 3, A and B , are means ± SEMs of at least 4 individual donors. * P

    Article Snippet: Proteins were separated on an 8% SDS-PAGE gel and transferred to a polyvinylidene difluoride membrane (Millipore), and immunoblotting was done with either α-Itk (Cell Signaling Technologies, Danvers, Mass) or α-β-actin (Sigma).

    Techniques: Expressing, Transduction, shRNA, Quantitative RT-PCR

    Effect of silibinin treatment on the gene expression of NER regulators and GADD45α in UVB-exposed NHDFs NHDFs were plated and divided into two groups: pre and post-UVB treatment. In the pre-UVB treatment group, cells were treated with silibinin and/or pifithrin for 3 hrs, then washed and exposed to UVB 1 mJ/cm 2 . Treatment with silibinin or pifithrin alone served as relevant controls. In the post-UVB group, cells were exposed first to UVB 1 mJ/cm 2 and then immediately treated with silibinin and/or pifithrin for 3 hrs. At the end of the described treatments, the mRNA levels of A. XPA, B. XPB, C. XPC, D. XPG and E. GADD45α were measured by real-time RT-PCR as described in methods. Samples were run in triplicate, and three independent experiments were performed for every gene of interest. The mRNA level of gene was normalized to those of β-actin mRNA in each sample. *; p

    Journal: Oncotarget

    Article Title: Silibinin enhances the repair of ultraviolet B-induced DNA damage by activating p53-dependent nucleotide excision repair mechanism in human dermal fibroblasts

    doi:

    Figure Lengend Snippet: Effect of silibinin treatment on the gene expression of NER regulators and GADD45α in UVB-exposed NHDFs NHDFs were plated and divided into two groups: pre and post-UVB treatment. In the pre-UVB treatment group, cells were treated with silibinin and/or pifithrin for 3 hrs, then washed and exposed to UVB 1 mJ/cm 2 . Treatment with silibinin or pifithrin alone served as relevant controls. In the post-UVB group, cells were exposed first to UVB 1 mJ/cm 2 and then immediately treated with silibinin and/or pifithrin for 3 hrs. At the end of the described treatments, the mRNA levels of A. XPA, B. XPB, C. XPC, D. XPG and E. GADD45α were measured by real-time RT-PCR as described in methods. Samples were run in triplicate, and three independent experiments were performed for every gene of interest. The mRNA level of gene was normalized to those of β-actin mRNA in each sample. *; p

    Article Snippet: Genomic DNA and mRNA purification kit, specific primers for human NER factors (XPA, XPB, XPC, XPG, DDB1 and DDB2), p53 and GADD45α as well as PCR-master mix were obtained from Qiagen (Germantown, MD). β-actin primers were obtained from Sigma (St. Louis, MO).

    Techniques: Expressing, Quantitative RT-PCR

    AXL-APTAMER Inhibits Downstream Signaling in OC Cells (A) pAXL levels after AXL-APTAMER treatment detected by western blot alaysis. SKOV3-IP OC cells were treated for 3 hr with a control aptamer or AXL-APTAMER. Cell lysates were immunoprecipitated with pAXL. β-actin was used as the housekeeping protein. Quantification of the bands was performed using ImageJ software. (B) Cell viability was detected by MTS assay for SKOV3-IP1 and HeyA8 cell line using three different time points (24, 48, and 72 hr). All experiments were performed in six replicates, and the results were reported as the mean absorption ± SEM (**p

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Therapeutic Targeting of AXL Receptor Tyrosine Kinase Inhibits Tumor Growth and Intraperitoneal Metastasis in Ovarian Cancer Models

    doi: 10.1016/j.omtn.2017.06.023

    Figure Lengend Snippet: AXL-APTAMER Inhibits Downstream Signaling in OC Cells (A) pAXL levels after AXL-APTAMER treatment detected by western blot alaysis. SKOV3-IP OC cells were treated for 3 hr with a control aptamer or AXL-APTAMER. Cell lysates were immunoprecipitated with pAXL. β-actin was used as the housekeeping protein. Quantification of the bands was performed using ImageJ software. (B) Cell viability was detected by MTS assay for SKOV3-IP1 and HeyA8 cell line using three different time points (24, 48, and 72 hr). All experiments were performed in six replicates, and the results were reported as the mean absorption ± SEM (**p

    Article Snippet: Protein β-actin (Sigma-Aldrich) was used as a loading control.

    Techniques: Western Blot, Immunoprecipitation, Software, MTS Assay

    (a) Western blotting for EGFR (170 kDa) and Beclin1 (60 kDa) in representative H-EGFR/L-Beclin1 (lanes 1 and 2), L-EGFR/H-Beclin1 (lanes 3–5), H-EGFR/H-Beclin1 (lanes 6–8), and L-EGFR/L-Beclin1 (lanes 9–11) GBs. (b) Up- or downregulation of either EGFR or Beclin1 is quantified by densitometric data analysis, which shows the relative expression of EGFR and Beclin1 after normalization to the  β -actin bands. Data are reported as means ± S.E. of three densitometric analyses of the same sample.

    Journal: BioMed Research International

    Article Title: Combined Epidermal Growth Factor Receptor and Beclin1 Autophagic Protein Expression Analysis Identifies Different Clinical Presentations, Responses to Chemo- and Radiotherapy, and Prognosis in Glioblastoma

    doi: 10.1155/2015/208076

    Figure Lengend Snippet: (a) Western blotting for EGFR (170 kDa) and Beclin1 (60 kDa) in representative H-EGFR/L-Beclin1 (lanes 1 and 2), L-EGFR/H-Beclin1 (lanes 3–5), H-EGFR/H-Beclin1 (lanes 6–8), and L-EGFR/L-Beclin1 (lanes 9–11) GBs. (b) Up- or downregulation of either EGFR or Beclin1 is quantified by densitometric data analysis, which shows the relative expression of EGFR and Beclin1 after normalization to the β -actin bands. Data are reported as means ± S.E. of three densitometric analyses of the same sample.

    Article Snippet: Membranes were blocked with 3% nonfat milk (BioRad) in PBS Tween 0.05% (PBST) and incubated overnight at 4°C with the following antibodies: anti-EGFR (undiluted, Novocastra), anti-Beclin1 (1 : 270, Sigma-Aldrich), and anti-β -actin (1 : 500, catalogue number: 04-1116, MERCK Millipore Corporation, Billerica, MA, US) diluted with 3% nonfat milk in PBST.

    Techniques: Western Blot, Expressing

    Stability of mutant core proteins. Western blot analysis of lysates harvested at 48 h postelectroporation. Results for core (top panels), NS5A (middle panels), and β-actin (lower panels) are shown. Residues mutated to alanine are identified. The

    Journal: Journal of Virology

    Article Title: Alanine Scanning of the Hepatitis C Virus Core Protein Reveals Numerous Residues Essential for Production of Infectious Virus ▿

    doi: 10.1128/JVI.00793-07

    Figure Lengend Snippet: Stability of mutant core proteins. Western blot analysis of lysates harvested at 48 h postelectroporation. Results for core (top panels), NS5A (middle panels), and β-actin (lower panels) are shown. Residues mutated to alanine are identified. The

    Article Snippet: For β-actin detection, mouse anti-β-actin MAb (Sigma, St. Louis, MO) was diluted 1:5,000 in TBST.

    Techniques: Mutagenesis, Western Blot

    Increased ACF mRNA and protein expression in Ob/Ob liver. A . The abundance of Ob/Ob hepatic apoB, apobec-1 and acf mRNA relative to that quantified from lean control liver was assessed by real time PCR and normalized to β-actin mRNA. Lean animal

    Journal: Biochimica et biophysica acta

    Article Title: Metabolic Regulation of APOBEC-1 Complementation Factor Trafficking in Mouse Models of Obesity and its Positive Correlation with the Expression of ApoB Protein in Hepatocytes

    doi: 10.1016/j.bbadis.2010.06.003

    Figure Lengend Snippet: Increased ACF mRNA and protein expression in Ob/Ob liver. A . The abundance of Ob/Ob hepatic apoB, apobec-1 and acf mRNA relative to that quantified from lean control liver was assessed by real time PCR and normalized to β-actin mRNA. Lean animal

    Article Snippet: Whole liver extracts were prepared by Teflon to glass homogenization of liver in 0.25 STM (0.25 M sucrose, 50 mM Tris pH 7.0 and 5 mM MgCl2 ) supplemented with Complete Protease Inhibitors (Roche, Indianapolis, IN) and phosphatase inhibitors, PhosStop (Roche) then cleared at 3,000 X g for 10 min. Nuclear and cytoplasmic preparations were centrifuged as previously described [ ] and the quality of the separation was assessed by western blotting extracts for cytoplasmic β-actin (Sigma) and nuclear histone H1 (Santa Cruz Biotechnology, Santa Cruz, CA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Enhanced ACF abundance supports increases in intracellular ApoB protein. A. Real time PCR was performed on poly A+ purified RNA from cytoplasmic and nuclear subcellular extracts of Ob/Ob and cogenic control animal liver. β-actin mRNA was quantified

    Journal: Biochimica et biophysica acta

    Article Title: Metabolic Regulation of APOBEC-1 Complementation Factor Trafficking in Mouse Models of Obesity and its Positive Correlation with the Expression of ApoB Protein in Hepatocytes

    doi: 10.1016/j.bbadis.2010.06.003

    Figure Lengend Snippet: Enhanced ACF abundance supports increases in intracellular ApoB protein. A. Real time PCR was performed on poly A+ purified RNA from cytoplasmic and nuclear subcellular extracts of Ob/Ob and cogenic control animal liver. β-actin mRNA was quantified

    Article Snippet: Whole liver extracts were prepared by Teflon to glass homogenization of liver in 0.25 STM (0.25 M sucrose, 50 mM Tris pH 7.0 and 5 mM MgCl2 ) supplemented with Complete Protease Inhibitors (Roche, Indianapolis, IN) and phosphatase inhibitors, PhosStop (Roche) then cleared at 3,000 X g for 10 min. Nuclear and cytoplasmic preparations were centrifuged as previously described [ ] and the quality of the separation was assessed by western blotting extracts for cytoplasmic β-actin (Sigma) and nuclear histone H1 (Santa Cruz Biotechnology, Santa Cruz, CA).

    Techniques: Real-time Polymerase Chain Reaction, Purification

    Acute leptin treatment suppresses acf mRNA abundance. The relative abundance of acf mRNA in primary mouse hepatocytes, normalized to β-actin mRNA abundance was determined by real time PCR following 4 hours of treatment with increasing concentrations

    Journal: Biochimica et biophysica acta

    Article Title: Metabolic Regulation of APOBEC-1 Complementation Factor Trafficking in Mouse Models of Obesity and its Positive Correlation with the Expression of ApoB Protein in Hepatocytes

    doi: 10.1016/j.bbadis.2010.06.003

    Figure Lengend Snippet: Acute leptin treatment suppresses acf mRNA abundance. The relative abundance of acf mRNA in primary mouse hepatocytes, normalized to β-actin mRNA abundance was determined by real time PCR following 4 hours of treatment with increasing concentrations

    Article Snippet: Whole liver extracts were prepared by Teflon to glass homogenization of liver in 0.25 STM (0.25 M sucrose, 50 mM Tris pH 7.0 and 5 mM MgCl2 ) supplemented with Complete Protease Inhibitors (Roche, Indianapolis, IN) and phosphatase inhibitors, PhosStop (Roche) then cleared at 3,000 X g for 10 min. Nuclear and cytoplasmic preparations were centrifuged as previously described [ ] and the quality of the separation was assessed by western blotting extracts for cytoplasmic β-actin (Sigma) and nuclear histone H1 (Santa Cruz Biotechnology, Santa Cruz, CA).

    Techniques: Real-time Polymerase Chain Reaction

    mRNA expression in callus tissue of control and PDLLA treated rats 2, 4 and 6 weeks post-surgery. Expression level of (A) Runx2, (B) HIF2A, (C) Wnt4, (D) Wnt10b, (E) GSK-3β, (F) β-catenin and (G) TCF-1. Β-actin is a control. Values are presented as mean ± standard deviation, *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Increased Runx2 expression associated with enhanced Wnt signaling in PDLLA internal fixation for fracture treatment

    doi: 10.3892/etm.2017.4216

    Figure Lengend Snippet: mRNA expression in callus tissue of control and PDLLA treated rats 2, 4 and 6 weeks post-surgery. Expression level of (A) Runx2, (B) HIF2A, (C) Wnt4, (D) Wnt10b, (E) GSK-3β, (F) β-catenin and (G) TCF-1. Β-actin is a control. Values are presented as mean ± standard deviation, *P

    Article Snippet: The β-actin protein (primary antibody from EMD Millipore, working concentration: 1:1,000) was blotted in the same membrane as an internal control to normalize the relative density.

    Techniques: Expressing, Standard Deviation

    Protein expression in callus tissue of control and PDLLA treated rats 2, 4 and 6 weeks post-surgery. Expression level of (A) Runx2, (B) HIF2A, (C) Wnt4, (D) Wnt10b, (E) GSK-3β, (F) β-catenin and (G) TCF-1 under PDLLA intramedullary fixation (PDLLA group) compared to that of Kirschner wire (control group). Β-actin is a control. Values are presented as mean ± standard deviation, *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Increased Runx2 expression associated with enhanced Wnt signaling in PDLLA internal fixation for fracture treatment

    doi: 10.3892/etm.2017.4216

    Figure Lengend Snippet: Protein expression in callus tissue of control and PDLLA treated rats 2, 4 and 6 weeks post-surgery. Expression level of (A) Runx2, (B) HIF2A, (C) Wnt4, (D) Wnt10b, (E) GSK-3β, (F) β-catenin and (G) TCF-1 under PDLLA intramedullary fixation (PDLLA group) compared to that of Kirschner wire (control group). Β-actin is a control. Values are presented as mean ± standard deviation, *P

    Article Snippet: The β-actin protein (primary antibody from EMD Millipore, working concentration: 1:1,000) was blotted in the same membrane as an internal control to normalize the relative density.

    Techniques: Expressing, Standard Deviation