Article Title: 28-Hydroxy-3-oxoolean-12-en-29-oic Acid, a Triterpene Acid from Celastrus Orbiculatus Extract, Inhibits the Migration and Invasion of Human Gastric Cancer Cells In Vitro
Figure Lengend Snippet: ( A , C ) Changes in PI3K/AKt/Snail biomarker expression levels following treatment with 28-hydroxy-3-oxoolean-12-en-29-oic acid for 24 h were assessed by Western blotting. ( B , D ) The band intensities of AKt, (p)-Akt, PI3K, (p)-PI3K and Snail relative to untreated control cells were quantified upon normalizing to β-actin expression, and are expressed as the mean ± standard deviation of three independent experiments.
Article Snippet: Reagents The reagents used included the following: RPMI 1640 cell culture medium, trypsin (HyClone, USA); fetal bovine serum (FBS) (Gibco, Waltham, MA, USA); artificially reconstituted basement membrane, Transwell chambers (Corning, Corning, NY, USA); thiazole blue (MTT) powder (Sigma, St. Louis, MO, USA); antibodies specific for E-cadherin, N-cadherin, vimentin, Snail, MMP-2, MMP-9, Akt, p-AKt, PI3K, p-PI3K, and β-actin (β-actin) (Cell Signaling, Danvers, MA, USA); and HRP-labeled goat anti-rabbit immunoglobulin (Ig) G (Hangzhou Huaan Biotechnology Co., Ltd., Hangzhou, China).
Techniques: Biomarker Assay, Expressing, Western Blot, Standard Deviation