β mercaptoethanol Millipore Search Results


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  • 99
    Millipore β mercaptoethanol β me
    Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; <t>β-ME,</t> <t>β-mercaptoethanol;</t> dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC
    β Mercaptoethanol β Me, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA β mercaptoethanol
    Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; <t>β-ME,</t> <t>β-mercaptoethanol;</t> dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC
    β Mercaptoethanol, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 428 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore millipore sigma β mercaptoethanol
    Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; <t>β-ME,</t> <t>β-mercaptoethanol;</t> dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC
    Millipore Sigma β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 2xsds β mercaptoethanol
    Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; <t>β-ME,</t> <t>β-mercaptoethanol;</t> dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC
    2xsds β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol bme
    Chemical structures and electrospray product ion tandem mass spectra of the natural chemoprevention agents used in this study and their corresponding adducts with <t>β-mercaptoethanol</t> <t>(BME).</t> A) isoliquiritigenin; B) isoliquiritigenin-BME adduct;
    β Mercaptoethanol Bme, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol βme
    Chemical structures and electrospray product ion tandem mass spectra of the natural chemoprevention agents used in this study and their corresponding adducts with <t>β-mercaptoethanol</t> <t>(BME).</t> A) isoliquiritigenin; B) isoliquiritigenin-BME adduct;
    β Mercaptoethanol βme, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol pbst
    Chemical structures and electrospray product ion tandem mass spectra of the natural chemoprevention agents used in this study and their corresponding adducts with <t>β-mercaptoethanol</t> <t>(BME).</t> A) isoliquiritigenin; B) isoliquiritigenin-BME adduct;
    β Mercaptoethanol Pbst, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore α β me atp
    Typical recording of arterial blood pressure (ABP) response to arterial injection of <t>α,β-me-ATP;</t> 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response
    α β Me Atp, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 2 β me
    Typical recording of arterial blood pressure (ABP) response to arterial injection of <t>α,β-me-ATP;</t> 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response
    2 β Me, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore reagent β mercaptoethanol
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
    Reagent β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore buffer rlt β mercaptoethanol
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
    Buffer Rlt β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol molecular grade
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
    β Mercaptoethanol Molecular Grade, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore photoprotective agents β mercaptoethanol
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
    Photoprotective Agents β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol laemmli buffer
    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with <t>β-mercaptoethanol;</t> 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).
    β Mercaptoethanol Laemmli Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; β-ME, β-mercaptoethanol; dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC

    Journal: Stem Cell Research & Therapy

    Article Title: Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function

    doi: 10.1186/s13287-018-0884-3

    Figure Lengend Snippet: Identification of adipose-derived stem cells (ASCs) and schematic of the experimental design. a Primary ASCs grew in clusters and had a rounded spindle-like shape. b ASCs at passage 2 could differentiate into adipocytes, and the visual field in photographs was filled with red lipid droplets stained with Oil Red O solution. c ASCs differentiated into osteocytes formed calcium nodules with burrs, which were stained red by Alizarin Red solution. d In cartilage pellets, many cartilage lacunae were found, and the glycosaminoglycans around the chondrocytes were stained purple-blue by Toluidine Blue O solution. e Flow cytometry showed that more than 98% of ASCs were immunopositive for the MSC markers CD29, CD44, and CD90, while less than 6% of ASCs were immunopositive for the hematopoietic stem cell markers CD34, CD45, and CD86. f Schematic showing the experimental groups and induction methods. bFGF, basic fibroblast growth factor; β-ME, β-mercaptoethanol; dASC, differentiated ASC; DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SC, Schwann cell; uASC, undifferentiated ASC

    Article Snippet: The experimental grouping and specific induction methods were as follows (Fig. ): (i) uASCs: undifferentiated ASCs (uASCs) at passage 2 were used; (ii–v) ASCs at passage 2 were pre-induced for 24 h with pre-induction medium I consisting of serum-free Dulbecco’s modified Eagle’s medium (DMEM) containing 1 mM β-mercaptoethanol (β-ME) (M3148; Sigma).

    Techniques: Derivative Assay, Staining, Flow Cytometry, Cytometry, Modification

    pH dependence of Chl d chemical synthesis from Chl a . Chromatograms (spectrum maximum plots) of detergent solubilised Chl a reacted with β-mercaptoethanol and heme in a 50 mM Tris buffer at the indicated pH. Unreacted Chl a , Chl d and 3 1 -sulfoxide of β-mercaptoethanol derivatives (a_I and a_II) are marked. Proportions of the major products and remaining Chl a and Chl a ’ (the C13 2 -Chl a epimer) are indicated in the pie charts for each pH tested.

    Journal: Scientific Reports

    Article Title: In vitro Conversion of Vinyl to Formyl Groups in Naturally Occurring Chlorophylls

    doi: 10.1038/srep06069

    Figure Lengend Snippet: pH dependence of Chl d chemical synthesis from Chl a . Chromatograms (spectrum maximum plots) of detergent solubilised Chl a reacted with β-mercaptoethanol and heme in a 50 mM Tris buffer at the indicated pH. Unreacted Chl a , Chl d and 3 1 -sulfoxide of β-mercaptoethanol derivatives (a_I and a_II) are marked. Proportions of the major products and remaining Chl a and Chl a ’ (the C13 2 -Chl a epimer) are indicated in the pie charts for each pH tested.

    Article Snippet: Reaction conditions Under standard reaction conditions, 10 µl of acetone dissolved chlorophyll was added to 3 ml of 0.02% (w/v) n-dodecyl-β-D-maltoside (Sigma) or 0.02% (v/v/) Triton X-100 in 50 mM Tris buffer, pH 8.0 plus 10 µM hemin chloride (MP Biochemicals) and 50 mM β-mercaptoethanol (Sigma).

    Techniques:

    Substitutions at the C3 position of ring A of Chl a mediated by β-mercaptoethanol. The C3 vinyl position of Chl a is targeted by the thiol group of β-mercaptoethanol yielding a sulfoxide derivative of [3 1 -β-mercaptoethanol] Chl a (product a_II), which can react further with β-mercaptoethanol to yield an unkown product (a_I) with a mass of a_II plus 76 Da. An alternate, competing reaction also targets the C3 vinyl group of Chl a , oxidatively cleaving it to yield [3-formyl]-Chl a (Chl d ). As is the case for product a_II, [3-formyl]-Chl a also reacts further with β-mercaptoethanol to yield an unknown product (a_III) with a mass of [3-formyl]-Chl a plus 76 Da. Structure of product a_II and synthesized Chl d is confirmed by NMR anaylsis (details see supplementary information ).

    Journal: Scientific Reports

    Article Title: In vitro Conversion of Vinyl to Formyl Groups in Naturally Occurring Chlorophylls

    doi: 10.1038/srep06069

    Figure Lengend Snippet: Substitutions at the C3 position of ring A of Chl a mediated by β-mercaptoethanol. The C3 vinyl position of Chl a is targeted by the thiol group of β-mercaptoethanol yielding a sulfoxide derivative of [3 1 -β-mercaptoethanol] Chl a (product a_II), which can react further with β-mercaptoethanol to yield an unkown product (a_I) with a mass of a_II plus 76 Da. An alternate, competing reaction also targets the C3 vinyl group of Chl a , oxidatively cleaving it to yield [3-formyl]-Chl a (Chl d ). As is the case for product a_II, [3-formyl]-Chl a also reacts further with β-mercaptoethanol to yield an unknown product (a_III) with a mass of [3-formyl]-Chl a plus 76 Da. Structure of product a_II and synthesized Chl d is confirmed by NMR anaylsis (details see supplementary information ).

    Article Snippet: Reaction conditions Under standard reaction conditions, 10 µl of acetone dissolved chlorophyll was added to 3 ml of 0.02% (w/v) n-dodecyl-β-D-maltoside (Sigma) or 0.02% (v/v/) Triton X-100 in 50 mM Tris buffer, pH 8.0 plus 10 µM hemin chloride (MP Biochemicals) and 50 mM β-mercaptoethanol (Sigma).

    Techniques: Synthesized, Nuclear Magnetic Resonance

    Reaction of monovinyl chlorophylls with β-mercaptoethanol in aqueous and methanolic environments. (a) Spectrum maximum (maximum absorbance reading at the range of 630–710 nm) RP-HPLC chromatograms of the aqueous buffer reaction mixture after reaction with β-mercaptoethanol using purified Chl a , Chl d , Chl b or Chl f as reactant. Dotted vertical lines mark the retention time of product a_III and Chl d . For more details regarding products a_I, a_II, a_III, b_I and f_I refer to the text and Table 1 . (b) RP-HPLC chromatograms of Chl a prior to (top panel) and following (two middle panels) reaction with β-mercaptoethanol in methanol, detected at indicated wavelengths. Chromatogram of Chl d from Acaryochloris (lower chromatogram) is shown for comparison with the chemically synthesised [3-formyl]-Chl a (Chl d ). Dotted vertical line marks the retention time of Chl d and Chl a . (c) Online spectra of the major derivatives synthesised from Chl a . The absorption spectra of Chl a and Chl d are plotted and their Q y maxima are marked with vertical lines for comparison with synthesised products. Q y maxima are indicated (in nm).

    Journal: Scientific Reports

    Article Title: In vitro Conversion of Vinyl to Formyl Groups in Naturally Occurring Chlorophylls

    doi: 10.1038/srep06069

    Figure Lengend Snippet: Reaction of monovinyl chlorophylls with β-mercaptoethanol in aqueous and methanolic environments. (a) Spectrum maximum (maximum absorbance reading at the range of 630–710 nm) RP-HPLC chromatograms of the aqueous buffer reaction mixture after reaction with β-mercaptoethanol using purified Chl a , Chl d , Chl b or Chl f as reactant. Dotted vertical lines mark the retention time of product a_III and Chl d . For more details regarding products a_I, a_II, a_III, b_I and f_I refer to the text and Table 1 . (b) RP-HPLC chromatograms of Chl a prior to (top panel) and following (two middle panels) reaction with β-mercaptoethanol in methanol, detected at indicated wavelengths. Chromatogram of Chl d from Acaryochloris (lower chromatogram) is shown for comparison with the chemically synthesised [3-formyl]-Chl a (Chl d ). Dotted vertical line marks the retention time of Chl d and Chl a . (c) Online spectra of the major derivatives synthesised from Chl a . The absorption spectra of Chl a and Chl d are plotted and their Q y maxima are marked with vertical lines for comparison with synthesised products. Q y maxima are indicated (in nm).

    Article Snippet: Reaction conditions Under standard reaction conditions, 10 µl of acetone dissolved chlorophyll was added to 3 ml of 0.02% (w/v) n-dodecyl-β-D-maltoside (Sigma) or 0.02% (v/v/) Triton X-100 in 50 mM Tris buffer, pH 8.0 plus 10 µM hemin chloride (MP Biochemicals) and 50 mM β-mercaptoethanol (Sigma).

    Techniques: High Performance Liquid Chromatography, Purification

    Dithiothreitol and benzyl mercaptan also facilitate the conversion of the C3 vinyl of Chl a (a) and Chl b (b) to a formyl. RP-HPLC chromatograms (top panels) demonstrate retention times of the 3 1 -formyl derivatives of Chl a and Chl b were identical and are marked with vertical lines. Spectra (bottom panels) were identical to the 3 1 -formyl derivatives formed in the presence of β-mercaptoethanol (β-merc.). Note, in the case of the reaction of Chl a with benzyl mercaptan, another product spectrally similar to Chl a has a similar retention time to Chl d , causing the mixed online absorption spectrum. Q y for [3-formyl]-Chl a (Chl d ) and [3-formyl]-Chl b are marked. Dotted line, β-mercaptoethanol; dashed line, dithiothreitol; solid line, benzyl mercaptan.

    Journal: Scientific Reports

    Article Title: In vitro Conversion of Vinyl to Formyl Groups in Naturally Occurring Chlorophylls

    doi: 10.1038/srep06069

    Figure Lengend Snippet: Dithiothreitol and benzyl mercaptan also facilitate the conversion of the C3 vinyl of Chl a (a) and Chl b (b) to a formyl. RP-HPLC chromatograms (top panels) demonstrate retention times of the 3 1 -formyl derivatives of Chl a and Chl b were identical and are marked with vertical lines. Spectra (bottom panels) were identical to the 3 1 -formyl derivatives formed in the presence of β-mercaptoethanol (β-merc.). Note, in the case of the reaction of Chl a with benzyl mercaptan, another product spectrally similar to Chl a has a similar retention time to Chl d , causing the mixed online absorption spectrum. Q y for [3-formyl]-Chl a (Chl d ) and [3-formyl]-Chl b are marked. Dotted line, β-mercaptoethanol; dashed line, dithiothreitol; solid line, benzyl mercaptan.

    Article Snippet: Reaction conditions Under standard reaction conditions, 10 µl of acetone dissolved chlorophyll was added to 3 ml of 0.02% (w/v) n-dodecyl-β-D-maltoside (Sigma) or 0.02% (v/v/) Triton X-100 in 50 mM Tris buffer, pH 8.0 plus 10 µM hemin chloride (MP Biochemicals) and 50 mM β-mercaptoethanol (Sigma).

    Techniques: High Performance Liquid Chromatography

    RP-HPLC chromatogram and online absorption spectra of 8-vinyl Chl a formyl and sulfoxide of β-mercaptoethanol derivatives. (a) RP-HPLC spectrum maximum plot of an incomplete 8-vinyl Chl a reaction after 2 h. Products with either a C3 or C8 conversion (one conversion) are marked in blue, and those products where both the C3 and C8 vinyl have reacted (two conversions) are marked with red. (b) Online spectra of 8-vinyl Chl a and the major formyl and sulfoxide of β-mercaptoethanol derivatives. The Soret and Q y maxima of 8-vinyl Chl a are marked with vertical red lines. Spectra are arithmetically shifted for clarity. βME, sulfoxide of β-mercaptoethanol.

    Journal: Scientific Reports

    Article Title: In vitro Conversion of Vinyl to Formyl Groups in Naturally Occurring Chlorophylls

    doi: 10.1038/srep06069

    Figure Lengend Snippet: RP-HPLC chromatogram and online absorption spectra of 8-vinyl Chl a formyl and sulfoxide of β-mercaptoethanol derivatives. (a) RP-HPLC spectrum maximum plot of an incomplete 8-vinyl Chl a reaction after 2 h. Products with either a C3 or C8 conversion (one conversion) are marked in blue, and those products where both the C3 and C8 vinyl have reacted (two conversions) are marked with red. (b) Online spectra of 8-vinyl Chl a and the major formyl and sulfoxide of β-mercaptoethanol derivatives. The Soret and Q y maxima of 8-vinyl Chl a are marked with vertical red lines. Spectra are arithmetically shifted for clarity. βME, sulfoxide of β-mercaptoethanol.

    Article Snippet: Reaction conditions Under standard reaction conditions, 10 µl of acetone dissolved chlorophyll was added to 3 ml of 0.02% (w/v) n-dodecyl-β-D-maltoside (Sigma) or 0.02% (v/v/) Triton X-100 in 50 mM Tris buffer, pH 8.0 plus 10 µM hemin chloride (MP Biochemicals) and 50 mM β-mercaptoethanol (Sigma).

    Techniques: High Performance Liquid Chromatography

    Chemical structures and electrospray product ion tandem mass spectra of the natural chemoprevention agents used in this study and their corresponding adducts with β-mercaptoethanol (BME). A) isoliquiritigenin; B) isoliquiritigenin-BME adduct;

    Journal: Analytical Biochemistry

    Article Title: Screening for Natural Chemoprevention Agents that Modify Human Keap1

    doi: 10.1016/j.ab.2011.10.028

    Figure Lengend Snippet: Chemical structures and electrospray product ion tandem mass spectra of the natural chemoprevention agents used in this study and their corresponding adducts with β-mercaptoethanol (BME). A) isoliquiritigenin; B) isoliquiritigenin-BME adduct;

    Article Snippet: Isoliquiritigenin, naringenin, Tris-HCl, sodium chloride, glycerol, formic acid, dimethyl sulfoxide, and β-mercaptoethanol (BME) were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques:

    Typical recording of arterial blood pressure (ABP) response to arterial injection of α,β-me-ATP; 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion

    doi: 10.1152/ajpheart.00188.2011

    Figure Lengend Snippet: Typical recording of arterial blood pressure (ABP) response to arterial injection of α,β-me-ATP; 0.125 mM of α,β-me-ATP were injected. Arrows indicate a start of injections. Top : effects of NGF infusion on pressor response

    Article Snippet: Body temperature was continuously monitored and maintained at 37.5–38.5°C with a heating pad and external heating lamps. α,β-me-ATP (Sigma) was dissolved in saline and made at 0.0625, 0.125, and 0.25 mM.

    Techniques: Injection

    Sympathetic and cardiovascular responses to arterial injection of α,β-me-ATP.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion

    doi: 10.1152/ajpheart.00188.2011

    Figure Lengend Snippet: Sympathetic and cardiovascular responses to arterial injection of α,β-me-ATP.

    Article Snippet: Body temperature was continuously monitored and maintained at 37.5–38.5°C with a heating pad and external heating lamps. α,β-me-ATP (Sigma) was dissolved in saline and made at 0.0625, 0.125, and 0.25 mM.

    Techniques: Injection

    Typical recording of renal sympathetic nerve activity (RSNA) and arterial blood pressure (AP) responses to stimulation of P2X. Arrowheads indicate a start of injections. Three dosages of α,β-methylene ATP (α,β-me-ATP) were

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion

    doi: 10.1152/ajpheart.00188.2011

    Figure Lengend Snippet: Typical recording of renal sympathetic nerve activity (RSNA) and arterial blood pressure (AP) responses to stimulation of P2X. Arrowheads indicate a start of injections. Three dosages of α,β-methylene ATP (α,β-me-ATP) were

    Article Snippet: Body temperature was continuously monitored and maintained at 37.5–38.5°C with a heating pad and external heating lamps. α,β-me-ATP (Sigma) was dissolved in saline and made at 0.0625, 0.125, and 0.25 mM.

    Techniques: Activity Assay

    Changes in RSNA and mean arterial pressure (MAP) in response to stimulation of afferent nerve P2X receptors. Three dosages of α,β-methylene ATP were injected into arterial blood supply of the hindlimb muscles of control rats ( n = 8) and

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion

    doi: 10.1152/ajpheart.00188.2011

    Figure Lengend Snippet: Changes in RSNA and mean arterial pressure (MAP) in response to stimulation of afferent nerve P2X receptors. Three dosages of α,β-methylene ATP were injected into arterial blood supply of the hindlimb muscles of control rats ( n = 8) and

    Article Snippet: Body temperature was continuously monitored and maintained at 37.5–38.5°C with a heating pad and external heating lamps. α,β-me-ATP (Sigma) was dissolved in saline and made at 0.0625, 0.125, and 0.25 mM.

    Techniques: Injection

    Top : effects of NGF infusion on blood pressure and heart rate responses to stimulation of P2X. α,β-me-ATP at 0.125 mM was injected into arterial blood supply of the muscles of control leg and experimental legs in 6 health rats. Control

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Contribution of nerve growth factor to upregulation of P2X3 expression in DRG neurons of rats with femoral artery occlusion

    doi: 10.1152/ajpheart.00188.2011

    Figure Lengend Snippet: Top : effects of NGF infusion on blood pressure and heart rate responses to stimulation of P2X. α,β-me-ATP at 0.125 mM was injected into arterial blood supply of the muscles of control leg and experimental legs in 6 health rats. Control

    Article Snippet: Body temperature was continuously monitored and maintained at 37.5–38.5°C with a heating pad and external heating lamps. α,β-me-ATP (Sigma) was dissolved in saline and made at 0.0625, 0.125, and 0.25 mM.

    Techniques: Injection

    In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with β-mercaptoethanol; 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).

    Journal: PLoS ONE

    Article Title: New Cysteine-Rich Ice-Binding Protein Secreted from Antarctic Microalga, Chloromonas sp.

    doi: 10.1371/journal.pone.0154056

    Figure Lengend Snippet: In-gel mobility of ChloroIBP. (A) Extracellular recombinant Trx-ChloroIBP visualized by immunoblotting following SDS-PAGE analysis in a redox experiment. 1, Trx-ChloroIBP treated with β-mercaptoethanol; 2, Trx-ChloroIBP oxidized by ambient air; 3, Trx-ChloroIBP alkylated by iodoacetamide after treatment with β-mercaptoethanol. (B) Topological change of native ChloroIBP. Treatment with reagents was the same as for (A). (C) Location of native ChloroIBP secreted into culture media on a native polyacrylamide gel. (M, protein markers representing bovine serum albumin based on the protein structure of P.69 pertactin (66) and L-lactic dehydrogenase (140); 1, silver staining of extracellular proteins secreted from Chloromonas sp.; 2, Periodic-acid staining of extracellular proteins from Chloromonas sp.; 3, Immunoblot band detected by anti-ChloroIBP antibody to a blot of Lane 1. (D) Topological movement of native ChloroIBP separated on a native polyacrylamide gel after reduction (Re) or under oxidizing conditions (Oxi).

    Article Snippet: Biochemical characteristics of native ChloroIBP The change of in-gel mobility of ChloroIBP by the reducing reagent β-mercaptoethanol (Sigma) was analyzed by immunoblotting with a ChloroIBP-specific polyclonal antibody.

    Techniques: Recombinant, SDS Page, Silver Staining, Staining