β glycerophosphate Search Results


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  • 99
    Thermo Fisher β glycerophosphate
    β Glycerophosphate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Thermo Fisher
    Average 99 stars, based on 799 article reviews
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    β glycerophosphate - by Bioz Stars, 2020-10
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    99
    Millipore β glycerophosphate
    BMP2 overcomes inhibited effect of MSC-CM on β-GP-induced VSMC calcification. a Microscopic views (×100) of Alizarin Red staining. b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. Expression of Msx2 ( d ), Runx2 ( e ), and osteocalcin ( f ) mRNA assessed by rt-PCR. g Cell lysates analyzed by western blot analysis with anti-Runx2 and p-Smad1/5/8. β-GP <t>beta-glycerophosphate,</t> DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2
    β Glycerophosphate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Millipore
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    93
    Santa Cruz Biotechnology β glycerophosphate
    ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and <t>β‐glycerophosphate</t> for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P
    β Glycerophosphate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Santa Cruz Biotechnology
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    93
    FUJIFILM β glycerophosphate
    Effect of toddaculin on osteoblast mineralization in ascorbic acid and <t>β-glycerophosphate-treated</t> MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P
    β Glycerophosphate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 93/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/FUJIFILM
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    97
    Millipore m β glycerophosphate
    Effect of toddaculin on osteoblast mineralization in ascorbic acid and <t>β-glycerophosphate-treated</t> MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P
    M β Glycerophosphate, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 696 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck & Co β glycerophosphate
    Effect of toddaculin on osteoblast mineralization in ascorbic acid and <t>β-glycerophosphate-treated</t> MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P
    β Glycerophosphate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Merck & Co
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    92
    Bio-Rad β glycerophosphate
    Effect of toddaculin on osteoblast mineralization in ascorbic acid and <t>β-glycerophosphate-treated</t> MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P
    β Glycerophosphate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Bio-Rad
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    92
    Merck KGaA β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Merck KGaA
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    93
    Nacalai β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate, supplied by Nacalai, used in various techniques. Bioz Stars score: 93/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Nacalai
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    93
    Valiant β glycerophosphate
    OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using <t>β-glycerophosphate</t> plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p
    β Glycerophosphate, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Valiant
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    90
    Applichem β glycerophosphate
    OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using <t>β-glycerophosphate</t> plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p
    β Glycerophosphate, supplied by Applichem, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Santa Cruz Biotechnology sodium β glycerophosphate
    OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using <t>β-glycerophosphate</t> plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p
    Sodium β Glycerophosphate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher β glycerolphosphate
    OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using <t>β-glycerophosphate</t> plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p
    β Glycerolphosphate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    BMP2 overcomes inhibited effect of MSC-CM on β-GP-induced VSMC calcification. a Microscopic views (×100) of Alizarin Red staining. b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. Expression of Msx2 ( d ), Runx2 ( e ), and osteocalcin ( f ) mRNA assessed by rt-PCR. g Cell lysates analyzed by western blot analysis with anti-Runx2 and p-Smad1/5/8. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: BMP2 overcomes inhibited effect of MSC-CM on β-GP-induced VSMC calcification. a Microscopic views (×100) of Alizarin Red staining. b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. Expression of Msx2 ( d ), Runx2 ( e ), and osteocalcin ( f ) mRNA assessed by rt-PCR. g Cell lysates analyzed by western blot analysis with anti-Runx2 and p-Smad1/5/8. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Modification, Derivative Assay

    Noggin, BMP2 antagonist, suppresses VSMC calcification by inhibiting Smad1/5/8 signaling. VSMCs pretreated with Noggin (100 ng/ml) or vehicle for 2 h followed by incubation with 10 mM β-GP. a Alizarin Red staining (×100). b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. mRNA expression of ( d ) BMP2, ( e ) Msx2, ( f ) Runx2, and ( g ) osteocalcin analyzed by rt-PCR. h Protein expression of BMP2 and phospho-Smad1/5/8 performed by western blot analysis. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, DMEM Noggin+β-GP DMEM medium containing 10 mM β-GP and 100 ng/ml Noggin, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: Noggin, BMP2 antagonist, suppresses VSMC calcification by inhibiting Smad1/5/8 signaling. VSMCs pretreated with Noggin (100 ng/ml) or vehicle for 2 h followed by incubation with 10 mM β-GP. a Alizarin Red staining (×100). b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. mRNA expression of ( d ) BMP2, ( e ) Msx2, ( f ) Runx2, and ( g ) osteocalcin analyzed by rt-PCR. h Protein expression of BMP2 and phospho-Smad1/5/8 performed by western blot analysis. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, DMEM Noggin+β-GP DMEM medium containing 10 mM β-GP and 100 ng/ml Noggin, Msx2 msh homeobox 2, NC DMEM medium, OD optical density, Runx2 Runt-related transcription factor 2

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: Incubation, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Modification

    β-GP induced calcium deposition in VSMCs in a dose-dependent manner. VSMCs cultured in normal medium or in calcification medium with 10, 20 and 40 mmol/l of β-GP for 14 days. a Alizarin Red staining of microscopic views (×100). b Formic acid 10% used to elute Alizarin Red dye and quantification of Alizarin Red staining measured with a microplate reader and normalized to total protein content. c Calcium content shown as mean ± standard deviation of three independent experiments conducted in duplicate. β-GP beta-glycerophosphate, OD optical density

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: β-GP induced calcium deposition in VSMCs in a dose-dependent manner. VSMCs cultured in normal medium or in calcification medium with 10, 20 and 40 mmol/l of β-GP for 14 days. a Alizarin Red staining of microscopic views (×100). b Formic acid 10% used to elute Alizarin Red dye and quantification of Alizarin Red staining measured with a microplate reader and normalized to total protein content. c Calcium content shown as mean ± standard deviation of three independent experiments conducted in duplicate. β-GP beta-glycerophosphate, OD optical density

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: Cell Culture, Staining, Standard Deviation

    MSC-CM inhibits β-GP-induced calcium deposition in VSMCs. Confluent cells incubated with normal medium or calcification medium with or without MSC-CM for 14 days. Calcification induced by addition of β-GP (10 mM). a Representative Alizarin Red staining of microscopic views (×100, upper) and plates (lower). b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, NC DMEM medium, OD optical density

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: MSC-CM inhibits β-GP-induced calcium deposition in VSMCs. Confluent cells incubated with normal medium or calcification medium with or without MSC-CM for 14 days. Calcification induced by addition of β-GP (10 mM). a Representative Alizarin Red staining of microscopic views (×100, upper) and plates (lower). b Quantification of Alizarin Red staining. c Calcium contents measured and normalized by protein content of cell lysates. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, NC DMEM medium, OD optical density

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: Incubation, Staining, Modification, Derivative Assay

    MSC-CM blocks BMP2–Smad1/5/8 signaling pathways. Confluent VSMCs incubated with different media in different groups for 7 days. a Effect of MSC-CM on BMP-2 mRNA expression. b Representative immunoblot of BMP2. c BMP2 protein quantified by densitometry. Levels of ( d ) BMPR1a, ( e ) BMPR1b, and ( f ) BMPR2 mRNA determined by rt-PCR. g Phosphorylation of Smad1/5/8 in whole cells. h Phosphorylation of Smad1/5/8 in nucleus and cytoplasm. β-GP beta-glycerophosphate, BMP-2 bone morphogenetic protein-2, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, NC DMEM medium

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: MSC-CM blocks BMP2–Smad1/5/8 signaling pathways. Confluent VSMCs incubated with different media in different groups for 7 days. a Effect of MSC-CM on BMP-2 mRNA expression. b Representative immunoblot of BMP2. c BMP2 protein quantified by densitometry. Levels of ( d ) BMPR1a, ( e ) BMPR1b, and ( f ) BMPR2 mRNA determined by rt-PCR. g Phosphorylation of Smad1/5/8 in whole cells. h Phosphorylation of Smad1/5/8 in nucleus and cytoplasm. β-GP beta-glycerophosphate, BMP-2 bone morphogenetic protein-2, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, NC DMEM medium

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: Incubation, Expressing, Reverse Transcription Polymerase Chain Reaction, Modification, Derivative Assay

    MSC-CM abolished β-GP-induced ALP activity and expression of specific osteogenic markers in VSMCs. Confluent VSMCs incubated with different media in different groups. a ALP activity measured after 3 days by ALP kit, normalized to cellular protein contents. Levels of Msx2 ( b ), Runx2 ( c ) and Osteocalcin ( d ) mRNA determined after 7 days by rt-PCR. e Representative immunoblot of Runx2. f Runx2 protein quantified by densitometry. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, Runx2 Runt-related transcription factor 2

    Journal: Stem Cell Research & Therapy

    Article Title: Conditioned medium from bone marrow-derived mesenchymal stem cells inhibits vascular calcification through blockade of the BMP2–Smad1/5/8 signaling pathway

    doi: 10.1186/s13287-018-0894-1

    Figure Lengend Snippet: MSC-CM abolished β-GP-induced ALP activity and expression of specific osteogenic markers in VSMCs. Confluent VSMCs incubated with different media in different groups. a ALP activity measured after 3 days by ALP kit, normalized to cellular protein contents. Levels of Msx2 ( b ), Runx2 ( c ) and Osteocalcin ( d ) mRNA determined after 7 days by rt-PCR. e Representative immunoblot of Runx2. f Runx2 protein quantified by densitometry. β-GP beta-glycerophosphate, DMEM Dulbecco’s Modified Eagle’s Medium, DMEM β-GP DMEM medium containing 10 mM β-GP, MSC-CM conditioned medium from bone marrow-derived mesenchymal stem cells, MSC-CM β-GP MSC-CM with 10 mM β-GP, Msx2 msh homeobox 2, NC DMEM medium, Runx2 Runt-related transcription factor 2

    Article Snippet: Beta-glycerophosphate (β-GP, G9422) and Alizarin Red S (A5533) were obtained from Sigma.

    Techniques: ALP Assay, Activity Assay, Expressing, Incubation, Reverse Transcription Polymerase Chain Reaction, Modification, Derivative Assay

    ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and β‐glycerophosphate for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P

    Journal: FEBS Open Bio

    Article Title: ADAMTS7 degrades Comp to fuel BMP2‐dependent osteogenic differentiation and ameliorate oncogenic potential in osteosarcomas

    doi: 10.1002/2211-5463.12939

    Figure Lengend Snippet: ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and β‐glycerophosphate for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P

    Article Snippet: Alizarin red staining MG63 cells were stimulated by 0.5 mm β‐glycerophosphate (#sc‐220452; Santa Cruz Biotechnology) and 50 mg·L−1 l‐ascorbic acid (#A92902; Sigma‐Aldrich, St Louis, MO, USA) for 21 days to induce osteogenic differentiation.

    Techniques: Staining

    Effect of toddaculin on osteoblast mineralization in ascorbic acid and β-glycerophosphate-treated MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P

    Journal: PLoS ONE

    Article Title: Toddaculin, Isolated from of Toddalia asiatica (L.) Lam., Inhibited Osteoclastogenesis in RAW 264 Cells and Enhanced Osteoblastogenesis in MC3T3-E1 Cells

    doi: 10.1371/journal.pone.0127158

    Figure Lengend Snippet: Effect of toddaculin on osteoblast mineralization in ascorbic acid and β-glycerophosphate-treated MC3T3-E1 cells that were evaluated by Alizarin red S staining (microplate reader analysis (A) and light microscopic evaluation (B)). Values are expressed as means ± SEM (n = 3). *, P

    Article Snippet: Ascorbic acid and β-glycerophosphate were purchased from Wako Pure Chemical (Osaka, Japan).

    Techniques: Staining

    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

    Journal: Scientific Reports

    Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

    doi: 10.1038/s41598-018-20241-3

    Figure Lengend Snippet: Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+ cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+ supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+ supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP, β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

    Article Snippet: Experimental design For calcification, medium was supplemented with 5% (v/v) FBS and 10 mM β -glycerophosphate (BGP, Merck Millipore, Massachusetts, USA).

    Techniques: Cell Culture, Isolation, Spectroscopy

    Impact of Mg 2+  on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+  (gray line). Of note: as the BGP culture supplemented with Mg 2+  contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP,  β -glycerophosphate.

    Journal: Scientific Reports

    Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

    doi: 10.1038/s41598-018-20241-3

    Figure Lengend Snippet: Impact of Mg 2+ on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+ (gray line). Of note: as the BGP culture supplemented with Mg 2+ contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP, β -glycerophosphate.

    Article Snippet: Experimental design For calcification, medium was supplemented with 5% (v/v) FBS and 10 mM β -glycerophosphate (BGP, Merck Millipore, Massachusetts, USA).

    Techniques: Cell Culture

    OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using β-glycerophosphate plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p

    Journal: Redox Biology

    Article Title: KLF2 regulates dental pulp-derived stem cell differentiation through the induction of mitophagy and altering mitochondrial metabolism

    doi: 10.1016/j.redox.2020.101622

    Figure Lengend Snippet: OB differentiation induces expression of KLF2 and autophagic genes. DPSCs were induced to differentiate into OBs using β-glycerophosphate plus l -ascorbic acid (BGP + LAA), and cells were harvested at various time points, whereas, undifferentiated cells were considered as a control. Quantitative RT-PCR was performed with the isolated RNA for measuring expressions of KLF2 and various autophagic genes during the course of differentiation. Star (*) indicates a statistical significance (p

    Article Snippet: Alizarin Red Solution (2003999) was obtained from Chemicon international. β Glycerophosphate (157241) was obtained from MP biomedical.

    Techniques: Expressing, Quantitative RT-PCR, Isolation

    Knockdown of KLF2 or ATG7 or BECN1 reduced autophagy-related molecules and OB differentiation-related markers. KLF2 or ATG7 or BECN1 was knocked down in DPSCs and cells were cultured in β-glycerophosphate plus l -ascorbic acid-containing medium. A. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of KLF2. B. MDC staining for detection of autophagy vesicles after knockdown of KLF2. C. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of ATG7. D. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of BECN1.

    Journal: Redox Biology

    Article Title: KLF2 regulates dental pulp-derived stem cell differentiation through the induction of mitophagy and altering mitochondrial metabolism

    doi: 10.1016/j.redox.2020.101622

    Figure Lengend Snippet: Knockdown of KLF2 or ATG7 or BECN1 reduced autophagy-related molecules and OB differentiation-related markers. KLF2 or ATG7 or BECN1 was knocked down in DPSCs and cells were cultured in β-glycerophosphate plus l -ascorbic acid-containing medium. A. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of KLF2. B. MDC staining for detection of autophagy vesicles after knockdown of KLF2. C. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of ATG7. D. Western blot analysis for detection of autophagy-related molecules and OB differentiation-related markers after knockdown of BECN1.

    Article Snippet: Alizarin Red Solution (2003999) was obtained from Chemicon international. β Glycerophosphate (157241) was obtained from MP biomedical.

    Techniques: Cell Culture, Western Blot, Staining