β funaltrexamine hydrochloride β fna Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Millipore β funaltrexamine hydrochloride β fna
    Antinociceptive effects of SK-9709 alone and in combination with <t>β-funaltrexamine</t> (β-FNA) (A) or nor-binaltorphimine (nor-BNI) (B) in the acetic acid-induced writhing test. Mice were treated with SK-9709 (1.93 μmol kg −1 , s.c.) 120 min before testing. (A) β-FNA (5.1 nmol per mouse, i.c.v.) and nor-BNI (4.9 nmol per mouse, i.t.) were injected 24 h and 25 min before testing, respectively. Acetic acid (0.7 %) was injected 10 min before writhing test, and then writhing responses were counted for 10 min. Each value is the mean±s.e.mean. The numbers of mice used are shown in parentheses. Significance levels; ** P
    β Funaltrexamine Hydrochloride β Fna, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β funaltrexamine hydrochloride β fna/product/Millipore
    Average 88 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    β funaltrexamine hydrochloride β fna - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    93
    Tocris beta funaltrexamine beta funaltrexamine β fna
    Effect of opioid receptor antagonists on PECN–induced antinociception assessed using the acetic acid–induced abdominal constriction test in mice. β <t>–funaltrexamine</t> <t>(β–FNA;</t> 10 mg/kg, i.p.), naltrindole (NALT; 1 mg/kg, i.p.), or nor–binaltorphimine (nor–BNI; 1 mg/kg, i.p.) were administered 90 min, 15 min and 30 min respectively, before oral administration of vehicle (10 mL/kg), or PECN (500 mg/kg). Each column represents the mean ± SEM of six mice. Statistical analyses were performed using one–way ANOVA followed by Dunnett’s post hoc test. ** p
    Beta Funaltrexamine Beta Funaltrexamine β Fna, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/beta funaltrexamine beta funaltrexamine β fna/product/Tocris
    Average 93 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    beta funaltrexamine beta funaltrexamine β fna - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    89
    Shionogi β funaltrexamine hydrochloride β fna
    Effect of opioid receptor antagonists on PECN–induced antinociception assessed using the acetic acid–induced abdominal constriction test in mice. β <t>–funaltrexamine</t> <t>(β–FNA;</t> 10 mg/kg, i.p.), naltrindole (NALT; 1 mg/kg, i.p.), or nor–binaltorphimine (nor–BNI; 1 mg/kg, i.p.) were administered 90 min, 15 min and 30 min respectively, before oral administration of vehicle (10 mL/kg), or PECN (500 mg/kg). Each column represents the mean ± SEM of six mice. Statistical analyses were performed using one–way ANOVA followed by Dunnett’s post hoc test. ** p
    β Funaltrexamine Hydrochloride β Fna, supplied by Shionogi, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β funaltrexamine hydrochloride β fna/product/Shionogi
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    β funaltrexamine hydrochloride β fna - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    Image Search Results


    Antinociceptive effects of SK-9709 alone and in combination with β-funaltrexamine (β-FNA) (A) or nor-binaltorphimine (nor-BNI) (B) in the acetic acid-induced writhing test. Mice were treated with SK-9709 (1.93 μmol kg −1 , s.c.) 120 min before testing. (A) β-FNA (5.1 nmol per mouse, i.c.v.) and nor-BNI (4.9 nmol per mouse, i.t.) were injected 24 h and 25 min before testing, respectively. Acetic acid (0.7 %) was injected 10 min before writhing test, and then writhing responses were counted for 10 min. Each value is the mean±s.e.mean. The numbers of mice used are shown in parentheses. Significance levels; ** P

    Journal: British Journal of Pharmacology

    Article Title: Long-lasting antinociceptive effects of a novel dynorphin analogue, Tyr-D-Ala-Phe-Leu-Arg ? (CH2NH) Arg-NH2, in mice

    doi: 10.1038/sj.bjp.0703982

    Figure Lengend Snippet: Antinociceptive effects of SK-9709 alone and in combination with β-funaltrexamine (β-FNA) (A) or nor-binaltorphimine (nor-BNI) (B) in the acetic acid-induced writhing test. Mice were treated with SK-9709 (1.93 μmol kg −1 , s.c.) 120 min before testing. (A) β-FNA (5.1 nmol per mouse, i.c.v.) and nor-BNI (4.9 nmol per mouse, i.t.) were injected 24 h and 25 min before testing, respectively. Acetic acid (0.7 %) was injected 10 min before writhing test, and then writhing responses were counted for 10 min. Each value is the mean±s.e.mean. The numbers of mice used are shown in parentheses. Significance levels; ** P

    Article Snippet: Morphine hydrochloride (Shionogi, Osaka, Japan), U-50,488H (trans-(±)3,4-dichloro-N-methyl-N-(2-[1-pyrrolidinyl]-cyclohexyl) benzeneacetamide methanesulphonate salt (Sigma, St. Louis, MO, U.S.A.), naloxone hydrochloride (Endo Lab., U.S.A.), β-funaltrexamine (β-FNA, Research Biochemicals International, MA, U.S.A.), nor-binaltorphimine (nor-BNI, Research Biochemicals International), DAMGO ([ D -Ala2 , (Me)Phe4 ,Gly(ol)5 ] enkephalin) and DPDPE ([D-Pen2 ,D-Pen5 ] enkephalin) were used.

    Techniques: Mouse Assay, Injection

    Effect of opioid receptor antagonists on PECN–induced antinociception assessed using the acetic acid–induced abdominal constriction test in mice. β –funaltrexamine (β–FNA; 10 mg/kg, i.p.), naltrindole (NALT; 1 mg/kg, i.p.), or nor–binaltorphimine (nor–BNI; 1 mg/kg, i.p.) were administered 90 min, 15 min and 30 min respectively, before oral administration of vehicle (10 mL/kg), or PECN (500 mg/kg). Each column represents the mean ± SEM of six mice. Statistical analyses were performed using one–way ANOVA followed by Dunnett’s post hoc test. ** p

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Antinociceptive activity of petroleum ether fraction obtained from methanolic extract of Clinacanthus nutans leaves involves the activation of opioid receptors and NO-mediated/cGMP-independent pathway

    doi: 10.1186/s12906-019-2486-8

    Figure Lengend Snippet: Effect of opioid receptor antagonists on PECN–induced antinociception assessed using the acetic acid–induced abdominal constriction test in mice. β –funaltrexamine (β–FNA; 10 mg/kg, i.p.), naltrindole (NALT; 1 mg/kg, i.p.), or nor–binaltorphimine (nor–BNI; 1 mg/kg, i.p.) were administered 90 min, 15 min and 30 min respectively, before oral administration of vehicle (10 mL/kg), or PECN (500 mg/kg). Each column represents the mean ± SEM of six mice. Statistical analyses were performed using one–way ANOVA followed by Dunnett’s post hoc test. ** p

    Article Snippet: Drugs and chemicals The following drugs, namely acetylsalicylic acid (ASA), morphine hydrochloride (MOR), diazepam (DZP), l –arginine and 1H-[1, 2, 4] oxadiazole [4,3–a]quinoxaline–1–one (ODQ) were purchased from Sigma–Aldrich (St. Louis, MO, USA), while β–funaltrexamine hydrochloride (β–FNA), nor–binaltorphimine dihydrochloride (nor–BNI), and naltrindole hydrochloride (NALT) were purchased from Tocris Bioscience (Ellisville, Missouri, USA).

    Techniques: Mouse Assay

    Intracellular mechanisms of the functional turnover of μ receptors. (A) Bar histograms show the mean ± SEM of the percentage of ME‐induced effect immediately ( t 15 = 0) and 45 min after 15 min ( t 15 = 45) of β‐FNA (300 nM, 15 min) perfusion in different experimental conditions: control ( n = 6), in the presence of low‐calcium aCSF ( n = 6), during neuronal inhibition by NA (100 μM) ( n = 6), at low temperature ( n = 5) or during trafficking inhibition by brefeldin A (10 μM) ( n = 5). * P

    Journal: British Journal of Pharmacology

    Article Title: Characterization of functional μ opioid receptor turnover in rat locus coeruleus: an electrophysiological and immunocytochemical study) Characterization of functional μ opioid receptor turnover in rat locus coeruleus: an electrophysiological and immunocytochemical study

    doi: 10.1111/bph.13901

    Figure Lengend Snippet: Intracellular mechanisms of the functional turnover of μ receptors. (A) Bar histograms show the mean ± SEM of the percentage of ME‐induced effect immediately ( t 15 = 0) and 45 min after 15 min ( t 15 = 45) of β‐FNA (300 nM, 15 min) perfusion in different experimental conditions: control ( n = 6), in the presence of low‐calcium aCSF ( n = 6), during neuronal inhibition by NA (100 μM) ( n = 6), at low temperature ( n = 5) or during trafficking inhibition by brefeldin A (10 μM) ( n = 5). * P

    Article Snippet: For electrophysiological recordings, the following drugs were used (drug source): brefeldin A (Tocris Bioscience, Bristol, UK), EEDQ (Sigma‐Aldrich Química S.A., Madrid, Spain), β‐FNA hydrochloride (Tocris Bioscience), ME acetate salt (Bachem, Weil am Rhein, Germany) and (−)–NA (+)–bitartrate salt hydrate (Sigma‐Aldrich Química S.A.).

    Techniques: Functional Assay, Inhibition

    Scheme summarizing the experimental design for functional characterization of μ receptor (MOR) turnover. (A) Functional turnover of μ receptor ‐mediated effect was evaluated by analysing the recovery of ME (3.2 μM, 1 min) effect after complete inactivation of μ receptors with the irreversible alkylating blocker β‐FNA (300–800 nM, 30 min); opioid effect recovery was measured before (control effect), immediately after inactivation ( t 30 = 0) and, then, every 15 min over a period of 300 min ( t 30 = 15–300). (B) For the μ receptor turnover to be compared with that of α 2 ‐adrenoceptors, NA (100 μM, 1 min) effect was tested before (control effect), immediately after complete receptor inactivation with the irreversible antagonist EEDQ (10 μM, 30 min) ( t 30 = 0) and, then, every 15 min over 300 min after inactivation ( t 30 = 15–300). (C) Functional μ receptor turnover was further characterized by evaluating the recovery of ME (3.2 μM, 1 min) effect for 120 min after complete μ receptor inactivation with 15, 30, 45 or 60 min of β‐FNA administration ( t 15, 30, 45, 60 = 0–120). (D) The recovery of ME (3.2 μM, 1 min) effect was also evaluated for 120 min after completion of a second 15 min perfusion with β‐FNA following the recovery of the first pre‐application ( t 15(1) = 0–120 and t 15(2) = 0–120). (E) The possible mechanisms underlying μ receptor turnover were explored by perfusion with a low‐calcium (0.2 mM) aCSF (to block LC cell calcium‐dependent mechanisms), administration of the α 2 ‐adrenoceptor agonist NA (100 μM) (to inhibit LC neuron firing‐dependent mechanisms), lowering the temperature of the chamber to 22°C (to evaluate vesicle movement mechanisms) or administration of the protein transport inhibitor brefeldin A (10 μM) (to test vesicle trafficking mechanisms); these manipulations were applied during the period of functional recovery after β‐FNA application, but regular conditions were restored 3–5 min before testing ME effect (45 min).

    Journal: British Journal of Pharmacology

    Article Title: Characterization of functional μ opioid receptor turnover in rat locus coeruleus: an electrophysiological and immunocytochemical study) Characterization of functional μ opioid receptor turnover in rat locus coeruleus: an electrophysiological and immunocytochemical study

    doi: 10.1111/bph.13901

    Figure Lengend Snippet: Scheme summarizing the experimental design for functional characterization of μ receptor (MOR) turnover. (A) Functional turnover of μ receptor ‐mediated effect was evaluated by analysing the recovery of ME (3.2 μM, 1 min) effect after complete inactivation of μ receptors with the irreversible alkylating blocker β‐FNA (300–800 nM, 30 min); opioid effect recovery was measured before (control effect), immediately after inactivation ( t 30 = 0) and, then, every 15 min over a period of 300 min ( t 30 = 15–300). (B) For the μ receptor turnover to be compared with that of α 2 ‐adrenoceptors, NA (100 μM, 1 min) effect was tested before (control effect), immediately after complete receptor inactivation with the irreversible antagonist EEDQ (10 μM, 30 min) ( t 30 = 0) and, then, every 15 min over 300 min after inactivation ( t 30 = 15–300). (C) Functional μ receptor turnover was further characterized by evaluating the recovery of ME (3.2 μM, 1 min) effect for 120 min after complete μ receptor inactivation with 15, 30, 45 or 60 min of β‐FNA administration ( t 15, 30, 45, 60 = 0–120). (D) The recovery of ME (3.2 μM, 1 min) effect was also evaluated for 120 min after completion of a second 15 min perfusion with β‐FNA following the recovery of the first pre‐application ( t 15(1) = 0–120 and t 15(2) = 0–120). (E) The possible mechanisms underlying μ receptor turnover were explored by perfusion with a low‐calcium (0.2 mM) aCSF (to block LC cell calcium‐dependent mechanisms), administration of the α 2 ‐adrenoceptor agonist NA (100 μM) (to inhibit LC neuron firing‐dependent mechanisms), lowering the temperature of the chamber to 22°C (to evaluate vesicle movement mechanisms) or administration of the protein transport inhibitor brefeldin A (10 μM) (to test vesicle trafficking mechanisms); these manipulations were applied during the period of functional recovery after β‐FNA application, but regular conditions were restored 3–5 min before testing ME effect (45 min).

    Article Snippet: For electrophysiological recordings, the following drugs were used (drug source): brefeldin A (Tocris Bioscience, Bristol, UK), EEDQ (Sigma‐Aldrich Química S.A., Madrid, Spain), β‐FNA hydrochloride (Tocris Bioscience), ME acetate salt (Bachem, Weil am Rhein, Germany) and (−)–NA (+)–bitartrate salt hydrate (Sigma‐Aldrich Química S.A.).

    Techniques: Functional Assay, Blocking Assay

    Confocal laser scanning microscopy imaging of μ receptor‐immunoreactivity (ir) in the LC before and after β‐FNA perfusion. In the absence of β‐FNA (control condition, cont), μ receptor‐ir (white) is identified as patches distributed both in the cytoplasm (double arrow) and in association with the plasma membrane (single arrow) in TH‐containing neurons (grey). Immediately after treatment with ß‐FNA (300 nM, 15 min, t 15 = 0), μ receptor‐ir is markedly depleted from the neuronal membrane, but μ receptor‐immunoreactive puncta are still observed within the cytoplasm (double arrow). Forty five min later ( t 15 = 45), the μ receptor is present again in the cell membrane (single arrow). Immediately after ß‐FNA (300 nM, 60 min, t 60 = 0) application, μ receptor‐ir is not detected in the cell membrane or the cytoplasmic compartment. All images displayed were acquired with identical laser intensity and detector gain parameters.

    Journal: British Journal of Pharmacology

    Article Title: Characterization of functional μ opioid receptor turnover in rat locus coeruleus: an electrophysiological and immunocytochemical study) Characterization of functional μ opioid receptor turnover in rat locus coeruleus: an electrophysiological and immunocytochemical study

    doi: 10.1111/bph.13901

    Figure Lengend Snippet: Confocal laser scanning microscopy imaging of μ receptor‐immunoreactivity (ir) in the LC before and after β‐FNA perfusion. In the absence of β‐FNA (control condition, cont), μ receptor‐ir (white) is identified as patches distributed both in the cytoplasm (double arrow) and in association with the plasma membrane (single arrow) in TH‐containing neurons (grey). Immediately after treatment with ß‐FNA (300 nM, 15 min, t 15 = 0), μ receptor‐ir is markedly depleted from the neuronal membrane, but μ receptor‐immunoreactive puncta are still observed within the cytoplasm (double arrow). Forty five min later ( t 15 = 45), the μ receptor is present again in the cell membrane (single arrow). Immediately after ß‐FNA (300 nM, 60 min, t 60 = 0) application, μ receptor‐ir is not detected in the cell membrane or the cytoplasmic compartment. All images displayed were acquired with identical laser intensity and detector gain parameters.

    Article Snippet: For electrophysiological recordings, the following drugs were used (drug source): brefeldin A (Tocris Bioscience, Bristol, UK), EEDQ (Sigma‐Aldrich Química S.A., Madrid, Spain), β‐FNA hydrochloride (Tocris Bioscience), ME acetate salt (Bachem, Weil am Rhein, Germany) and (−)–NA (+)–bitartrate salt hydrate (Sigma‐Aldrich Química S.A.).

    Techniques: Confocal Laser Scanning Microscopy, Imaging

    Effects of the nonselective opioid receptor antagonist naloxone (A), selective μ-opioid receptor antagonist β-FNA (B), selective κ-opioid receptor antagonist nor-BNI (C), and selective ORL1 receptor antagonist J-113397 (D) on the

    Journal: Acta Pharmacologica Sinica

    Article Title: Pharmacological mechanisms underlying the antinociceptive and tolerance effects of the 6,14-bridged oripavine compound 030418

    doi: 10.1038/aps.2011.83

    Figure Lengend Snippet: Effects of the nonselective opioid receptor antagonist naloxone (A), selective μ-opioid receptor antagonist β-FNA (B), selective κ-opioid receptor antagonist nor-BNI (C), and selective ORL1 receptor antagonist J-113397 (D) on the

    Article Snippet: DAMGO, (±) , SNC80, N/OFQ, naloxone, nor-BNI, and GDP were purchased from Sigma Chemical Co (St Louis, MO, USA), while J-113397 and β-FNA were purchased from Tocris Bioscience (Bristol, UK).

    Techniques:

    Effects of the μ receptor antagonist β-Funaltrexamine on tail-flick latency after 1.5mg/kg morphine i.p. injection in HR vs. LR animals. Values are mean ± s.e.m. ( n =14-16 animals per group). *p

    Journal: Behavioural brain research

    Article Title: Differential responses to morphine-induced analgesia in the tail-flick test

    doi: 10.1016/j.bbr.2008.06.034

    Figure Lengend Snippet: Effects of the μ receptor antagonist β-Funaltrexamine on tail-flick latency after 1.5mg/kg morphine i.p. injection in HR vs. LR animals. Values are mean ± s.e.m. ( n =14-16 animals per group). *p

    Article Snippet: HR and LR rats were then assigned to two different treatment groups: saline or 3mg/kg β-funaltrexamine (β-funaltrexamine hydrochloride, Tocris, Ellisville, MO).

    Techniques: Tail Flick Test, Injection

    Methyl-orvinol (0.1– 1 mg/kg, injected ip ) elicited inhibitory effect on colonic bead expulsion time in mice (15, 45 and 90 min after injection) (A). Its inhibitory action was reversed by naloxone (1 mg/kg, ip ), β-funaltrexamine (β-FNA, 1 mg/kg, ip ) and by methiodide-naloxone (met-naloxone, 1 mg/kg, ip ) (B). Moreover, methyl-orvinol at the dose of 0.1 mg/kg, injected ip , prolonged whole GI transit time in mice (C). Data represent mean ± SEM of n = 8. * p

    Journal: Pharmacological reports : PR

    Article Title: Methyl-orvinol – dual activity opioid receptor ligand inhibits gastrointestinal transit and alleviates abdominal pain in the mouse models mimicking IBS-D

    doi: 10.1016/j.pharep.2016.12.001

    Figure Lengend Snippet: Methyl-orvinol (0.1– 1 mg/kg, injected ip ) elicited inhibitory effect on colonic bead expulsion time in mice (15, 45 and 90 min after injection) (A). Its inhibitory action was reversed by naloxone (1 mg/kg, ip ), β-funaltrexamine (β-FNA, 1 mg/kg, ip ) and by methiodide-naloxone (met-naloxone, 1 mg/kg, ip ) (B). Moreover, methyl-orvinol at the dose of 0.1 mg/kg, injected ip , prolonged whole GI transit time in mice (C). Data represent mean ± SEM of n = 8. * p

    Article Snippet: Naloxone hydrochloride, β-funaltrexamine hydrochloride were purchased from Tocris Bioscience (Ellisville, MO, USA).

    Techniques: Injection, Mouse Assay

    The effect of methyl-orvinol (0.1 mg/kg, ip ) alone and in the presence of β-funaltrexamine (β-FNA, 1 mg/kg, ip ) on a total number of behavioral pain responses induced by ic administration of mustard oil solution in mice. Data shown as ± SEM of n = 8 mice per group. *** p

    Journal: Pharmacological reports : PR

    Article Title: Methyl-orvinol – dual activity opioid receptor ligand inhibits gastrointestinal transit and alleviates abdominal pain in the mouse models mimicking IBS-D

    doi: 10.1016/j.pharep.2016.12.001

    Figure Lengend Snippet: The effect of methyl-orvinol (0.1 mg/kg, ip ) alone and in the presence of β-funaltrexamine (β-FNA, 1 mg/kg, ip ) on a total number of behavioral pain responses induced by ic administration of mustard oil solution in mice. Data shown as ± SEM of n = 8 mice per group. *** p

    Article Snippet: Naloxone hydrochloride, β-funaltrexamine hydrochloride were purchased from Tocris Bioscience (Ellisville, MO, USA).

    Techniques: Mouse Assay