Journal: Scientific Reports
Article Title: β-Eudesmol, an oxygenized sesquiterpene, stimulates appetite via TRPA1 and the autonomic nervous system
Figure Lengend Snippet: TRPA1 inhibitors and activators affected GVNA in rats and β-eudesmol activated rat TRPA1. ( A ) Representative traces of whole-cell patch-clamp currents activated by β-eudesmol (60 μM) in HEK293 cells expressing rat TRPA1. AITC (100 μM) was used as a TRPA1 stimulant. (a), (b) Indicate the points of ramp pulse application to generate curves I–V shown in ( B , C , D ). The effect of a selective TRPA1 inhibitor, HC-030031 (300 mg/kg), on β-eudesmol derived GVNA elevation (n = 3). ( E , F ). The effect of AP-18 (1 mg/kg), a selective TRPA1 inhibitor, on β-eudesmol derived GVNA elevation (n = 3). ( G , H ). The effect of a non-selective TRPA1 inhibitor, Ruthenium Red (1 mg/kg), on β-eudesmol derived GVNA elevation (n = 3). ( I , J ). The effect of a representative TRPA1 activator, AITC, on GVNA (n = 5). Values are means ± SEM. Statistical differences were analyzed by the Mann-Whitney U test. *p
Article Snippet: Materials β-Eudesmol was purchased from Wako Pure Chemical Industries (Osaka, Japan).
Techniques: Patch Clamp, Expressing, Derivative Assay, MANN-WHITNEY