Journal: Frontiers in Bioengineering and Biotechnology
Article Title: β-Elemene Reverses the Resistance of p53-Deficient Colorectal Cancer Cells to 5-Fluorouracil by Inducing Pro-death Autophagy and Cyclin D3-Dependent Cycle Arrest
Figure Lengend Snippet: β-elemene reverse the resistance of HCT116p53 –/– cells to 5-Fu by inducing pro-death autophagy. (A) HCT116p53 +/+ and HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the protein expression of LC3B, Beclin-1, and Active caspase-3 were examined by Western blot; (B) HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the autophagy flow was observed by immunofluorescence, red arrow indicates autolysosomes; (C) HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the occurrence of autophagy was observed by transmission electron microscope; (D) HCT116p53 –/– cells were treated with 5-Fu + β-elemene, 5-Fu + β-elemene + Bafilomycin A1, the cell viability was examined by CCK8. (*** p
Article Snippet: Anti-rabbit IgG, HRP-linked Antibody (#7074) and Anti-mouse IgG, HRP-linked Antibody (#7076) were also obtained from CST. β-elemene was obtained from LKT lab (E4418, purity ≥98%), β-elemene was dissolved in ethanol (02483, Supelco).
Techniques: Expressing, Western Blot, Immunofluorescence, Transmission Assay, Microscopy