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  • 94
    Millipore β elemene
    Typical chromatogram of 28‐day‐old plantlets of Brassica napus L. var. Es Astrid using selected ion monitoring (SIM) mode. Peak identification: 1: myrcene, 2: limonene, 3: n ‐butyl benzene internal standard (IS) not used, 4: <t>β‐elemene,</t> 5: octylbenzene (IS), 6: (E,E)‐α‐farnesene [Colour figure can be viewed at http://wileyonlinelibrary.com ]
    β Elemene, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β elemene/product/Millipore
    Average 94 stars, based on 63 article reviews
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    94
    Abcam β elemene
    Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- <t>β</t> -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p
    β Elemene, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β elemene/product/Abcam
    Average 94 stars, based on 7 article reviews
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    90
    LKT Laboratories β elemene
    Total ion chromatogram of the  n -hexane fractions separated from MO- ( a ) and WI-fed female ( b ) extracts. Enlarged chromatograms for each female are shown on the right. The peaks indicated with arrows were previously identified as contact sex pheromones 12 . Peaks 1 and 2 represent  β -elemene and  β -caryophyllene, respectively.
    β Elemene, supplied by LKT Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β elemene/product/LKT Laboratories
    Average 90 stars, based on 2 article reviews
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    93
    Dalian Huali Jingang Pharmaceutical Co Ltd β elemene
    <t>β-elemene</t> inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P
    β Elemene, supplied by Dalian Huali Jingang Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β elemene/product/Dalian Huali Jingang Pharmaceutical Co Ltd
    Average 93 stars, based on 14 article reviews
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    89
    CSPC Pharmaceutical Group β elemene
    Chemical structure of  β -Elemene.
    β Elemene, supplied by CSPC Pharmaceutical Group, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    ImmunoReagents β elemene
    <t>β-Elemene</t> blocks the cisplatin-induced increase in ERCC-1 protein in human ovarian tumor cells. A2780/CP70 cells were exposed to 40 μM cisplatin (CDDP) alone or cisplatin plus β-elemene (ELE) at the IC 20 for 24 or 48 h. The cells were harvested and lysed. Cell lysates containing 40 μg of protein were analyzed on western blots using ERCC-1 antiserum and antibody against β-actin (loading control), as described previously ( 23 , 24 ).
    β Elemene, supplied by ImmunoReagents, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β elemene/product/ImmunoReagents
    Average 89 stars, based on 1 article reviews
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    93
    Learning and Memory β elemene improved learning
    <t>β-Elemene</t> blocks the cisplatin-induced increase in ERCC-1 protein in human ovarian tumor cells. A2780/CP70 cells were exposed to 40 μM cisplatin (CDDP) alone or cisplatin plus β-elemene (ELE) at the IC 20 for 24 or 48 h. The cells were harvested and lysed. Cell lysates containing 40 μg of protein were analyzed on western blots using ERCC-1 antiserum and antibody against β-actin (loading control), as described previously ( 23 , 24 ).
    β Elemene Improved Learning, supplied by Learning and Memory, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Supelco β elemene
    <t>β-elemene</t> reverse the resistance of HCT116p53 –/– cells to 5-Fu by inducing pro-death autophagy. (A) HCT116p53 +/+ and HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the protein expression of LC3B, Beclin-1, and Active caspase-3 were examined by Western blot; (B) HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the autophagy flow was observed by immunofluorescence, red arrow indicates autolysosomes; (C) HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the occurrence of autophagy was observed by transmission electron microscope; (D) HCT116p53 –/– cells were treated with 5-Fu + β-elemene, 5-Fu + β-elemene + Bafilomycin A1, the cell viability was examined by CCK8. (*** p
    β Elemene, supplied by Supelco, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore materials β elemene
    The anti-proliferation and anti-migration effect assay in K30 cells when treated with <t>β-elemene,</t> DOX, dual drugs and bMED NPs: (A) Clony images of cells; (B) Quantitative data on the relative colony number; (C) The images of cells in transwell migration assay; (D) Quantitative data on transwell migration. Error bars represent the SD of the mean. * p
    Materials β Elemene, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/materials β elemene/product/Millipore
    Average 91 stars, based on 1 article reviews
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    Image Search Results


    Typical chromatogram of 28‐day‐old plantlets of Brassica napus L. var. Es Astrid using selected ion monitoring (SIM) mode. Peak identification: 1: myrcene, 2: limonene, 3: n ‐butyl benzene internal standard (IS) not used, 4: β‐elemene, 5: octylbenzene (IS), 6: (E,E)‐α‐farnesene [Colour figure can be viewed at http://wileyonlinelibrary.com ]

    Journal: Phytochemical Analysis

    Article Title: A laboratory high‐throughput glass chamber using dynamic headspace TD‐GC/MS method for the analysis of whole Brassica napus L. plantlet volatiles under cadmium‐related abiotic stress. A laboratory high‐throughput glass chamber using dynamic headspace TD‐GC/MS method for the analysis of whole Brassica napus L. plantlet volatiles under cadmium‐related abiotic stress

    doi: 10.1002/pca.2750

    Figure Lengend Snippet: Typical chromatogram of 28‐day‐old plantlets of Brassica napus L. var. Es Astrid using selected ion monitoring (SIM) mode. Peak identification: 1: myrcene, 2: limonene, 3: n ‐butyl benzene internal standard (IS) not used, 4: β‐elemene, 5: octylbenzene (IS), 6: (E,E)‐α‐farnesene [Colour figure can be viewed at http://wileyonlinelibrary.com ]

    Article Snippet: In this way representative single‐ion peaks with respective relative abundance of myrcene (23.03%), β‐elemene (7.29%) and (E,E)‐α‐farnesene (9.45%) were integrated and compared with the equivalent single‐ion response of 1 μL of hexane solution containing an internal standard of octylbenzene (0.5 mg/mL) (2.69%) (Sigma‐Aldrich).

    Techniques:

    Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p

    Journal: Proceedings of the Royal Society B: Biological Sciences

    Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

    doi: 10.1098/rspb.2017.1134

    Figure Lengend Snippet: Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p

    Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

    Techniques: Activity Assay

    GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)

    Journal: Proceedings of the Royal Society B: Biological Sciences

    Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

    doi: 10.1098/rspb.2017.1134

    Figure Lengend Snippet: GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)

    Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

    Techniques: Gas Chromatography-Mass Spectrometry

    Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi B. bassiana ( b ), Me. anisopliae ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi B. bassiana and Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p

    Journal: Proceedings of the Royal Society B: Biological Sciences

    Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

    doi: 10.1098/rspb.2017.1134

    Figure Lengend Snippet: Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi B. bassiana ( b ), Me. anisopliae ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi B. bassiana and Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p

    Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

    Techniques: Activity Assay

    Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test, p

    Journal: Proceedings of the Royal Society B: Biological Sciences

    Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

    doi: 10.1098/rspb.2017.1134

    Figure Lengend Snippet: Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test, p

    Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

    Techniques:

    The growth rate of A375 cell line was inhibited by ß-elemene. A375 cells were cultivated in 96-well plates at a density of 5×103 cells/well and treated with different concentrations of ß-elemene in different time periods: A. 24, B. 48, and C. 72 hours. Cell proliferation was evaluated using the MTT assay. The IC 50 value is a concentration of a drug that inhibits cell proliferation by 50% in comparison to the control. The data are shown as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: The growth rate of A375 cell line was inhibited by ß-elemene. A375 cells were cultivated in 96-well plates at a density of 5×103 cells/well and treated with different concentrations of ß-elemene in different time periods: A. 24, B. 48, and C. 72 hours. Cell proliferation was evaluated using the MTT assay. The IC 50 value is a concentration of a drug that inhibits cell proliferation by 50% in comparison to the control. The data are shown as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Article Snippet: ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: MTT Assay, Concentration Assay

    The capability of ß-elemene to inhibit cell proliferation was measured by the MTT assay. The viability of cells was approximately decreased in dose- and time-dependent manners. The difference in the IC 50 value for ß-elemene was observed among the different incubation times. A significant reduction was detected in the viability of treated cells in a 72 hours incubation time compared with 24 and 48 hour periods. The data are presented as the means ± SD of three independent experiments. IC 50 ; The half maximal inhibitory concentration.

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: The capability of ß-elemene to inhibit cell proliferation was measured by the MTT assay. The viability of cells was approximately decreased in dose- and time-dependent manners. The difference in the IC 50 value for ß-elemene was observed among the different incubation times. A significant reduction was detected in the viability of treated cells in a 72 hours incubation time compared with 24 and 48 hour periods. The data are presented as the means ± SD of three independent experiments. IC 50 ; The half maximal inhibitory concentration.

    Article Snippet: ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: MTT Assay, Incubation, Concentration Assay

    Cell proliferation was inhibited by ß-elemene. ß-elemene also increased the radiosensitivity of A375 cells. Comparison of the viability of A375 cells after the treatment with 40, 80 µg/ml of ß-elemene. After 24 hours of incubation time, cells were exposed to 2 and 4 (Gy) of 6 MV X-ray; then, the viability of cells was measured using the MTT assay. All treated groups were compared with the control group (treatment-naive). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant difference. *; P

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: Cell proliferation was inhibited by ß-elemene. ß-elemene also increased the radiosensitivity of A375 cells. Comparison of the viability of A375 cells after the treatment with 40, 80 µg/ml of ß-elemene. After 24 hours of incubation time, cells were exposed to 2 and 4 (Gy) of 6 MV X-ray; then, the viability of cells was measured using the MTT assay. All treated groups were compared with the control group (treatment-naive). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant difference. *; P

    Article Snippet: ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: Incubation, MTT Assay

    Annexin V-PI staining for the assessment of apoptosis following ß-elemene and radiation therapy in A375 human melanoma cell line. The pretreated process on cancer cells was performed at two concentrations of ß-elemene (40 and 80 µg/ml) for 24 hours. Then cells were exposed to 2 and 4 Gy irradiations in combination with ß-elemene for 24 hours. A. Early apoptosis was evaluated by Annexin V+/PI- staining, and Annexin V+/PI+ staining was applied as a marker for the detection of cells in the late apoptosis phase and B. PI and Annexin V double staining results indicated the induction of apoptosis by ß-elemene and enhanced radiation-induced apoptosis in human melanoma cancer cells. Cells were exposed to ß-elemene at concentrations of 40 and 80 µg/ml along with 2 and 4 Gy irradiations. All the treatment groups were compared with the control group (no drug). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: Annexin V-PI staining for the assessment of apoptosis following ß-elemene and radiation therapy in A375 human melanoma cell line. The pretreated process on cancer cells was performed at two concentrations of ß-elemene (40 and 80 µg/ml) for 24 hours. Then cells were exposed to 2 and 4 Gy irradiations in combination with ß-elemene for 24 hours. A. Early apoptosis was evaluated by Annexin V+/PI- staining, and Annexin V+/PI+ staining was applied as a marker for the detection of cells in the late apoptosis phase and B. PI and Annexin V double staining results indicated the induction of apoptosis by ß-elemene and enhanced radiation-induced apoptosis in human melanoma cancer cells. Cells were exposed to ß-elemene at concentrations of 40 and 80 µg/ml along with 2 and 4 Gy irradiations. All the treatment groups were compared with the control group (no drug). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Article Snippet: ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: Staining, Marker, Double Staining

    Total ion chromatogram of the  n -hexane fractions separated from MO- ( a ) and WI-fed female ( b ) extracts. Enlarged chromatograms for each female are shown on the right. The peaks indicated with arrows were previously identified as contact sex pheromones 12 . Peaks 1 and 2 represent  β -elemene and  β -caryophyllene, respectively.

    Journal: Scientific Reports

    Article Title: Host plant affects the sexual attractiveness of the female white-spotted longicorn beetle, Anoplophora malasiaca

    doi: 10.1038/srep29526

    Figure Lengend Snippet: Total ion chromatogram of the n -hexane fractions separated from MO- ( a ) and WI-fed female ( b ) extracts. Enlarged chromatograms for each female are shown on the right. The peaks indicated with arrows were previously identified as contact sex pheromones 12 . Peaks 1 and 2 represent β -elemene and β -caryophyllene, respectively.

    Article Snippet: Effects of β -elemene and an MO-bark extract on WI-fed male rejection responses The candidate chemical, (−)-β -elemene (LKT Laboratories, Inc., St Paul, MN, USA; purity, 98.9%), was adjusted to 0.025, 0.1, 1, and 10 fe (1, 4, 40, and 400 ng per treatment, respectively) and applied to dummies along with the WI-fed female extract.

    Techniques:

    Effects of  β -elemene and an MO-bark extract on WI-fed male rejection responses. Response values accompanied by the same letter did not significantly differ at the  P  = 0.05 level ( n  × 2 chi-squared test and subsequent paired chi-squared test with Bonferroni-corrected  P- values). The values at the top of the graph indicate the number of individuals that responded out of all of the replicates (responded/replicates).

    Journal: Scientific Reports

    Article Title: Host plant affects the sexual attractiveness of the female white-spotted longicorn beetle, Anoplophora malasiaca

    doi: 10.1038/srep29526

    Figure Lengend Snippet: Effects of β -elemene and an MO-bark extract on WI-fed male rejection responses. Response values accompanied by the same letter did not significantly differ at the P  = 0.05 level ( n  × 2 chi-squared test and subsequent paired chi-squared test with Bonferroni-corrected P- values). The values at the top of the graph indicate the number of individuals that responded out of all of the replicates (responded/replicates).

    Article Snippet: Effects of β -elemene and an MO-bark extract on WI-fed male rejection responses The candidate chemical, (−)-β -elemene (LKT Laboratories, Inc., St Paul, MN, USA; purity, 98.9%), was adjusted to 0.025, 0.1, 1, and 10 fe (1, 4, 40, and 400 ng per treatment, respectively) and applied to dummies along with the WI-fed female extract.

    Techniques:

    β-elemene inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P

    Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Migration

    β-elemene induces apoptosis in SiHa cells. (A) Following treatment of SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to detect cellular apoptosis; *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene induces apoptosis in SiHa cells. (A) Following treatment of SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to detect cellular apoptosis; *P

    Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Flow Cytometry, Cytometry

    β-elemene inhibits the proliferation and survival of SiHa cells. Following treatment of SiHa cells with increasing doses of β-elemene (0–50 µg/ml) for 24, 48 and 72 h, the MTT assay was used to detect the proliferation and survival of SiHa cells. Each test was conducted in triplicate. Values presented represent the mean ± standard deviation of the three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene inhibits the proliferation and survival of SiHa cells. Following treatment of SiHa cells with increasing doses of β-elemene (0–50 µg/ml) for 24, 48 and 72 h, the MTT assay was used to detect the proliferation and survival of SiHa cells. Each test was conducted in triplicate. Values presented represent the mean ± standard deviation of the three independent experiments. *P

    Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: MTT Assay, Standard Deviation

    β-elemene induces cell cycle arrest in SiHa cells during G1. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to investigate the influence of β-elemene on the progression of the cell cycle; *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene induces cell cycle arrest in SiHa cells during G1. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to investigate the influence of β-elemene on the progression of the cell cycle; *P

    Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Flow Cytometry, Cytometry

    β-elemene suppresses the Wnt/β-catenin signaling pathway. Expression levels of β-catenin, TCF7, and c-Myc were detected via western blot analysis. Each experiment was performed in triplicate. Values presented represent the mean ± standard deviation of three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene suppresses the Wnt/β-catenin signaling pathway. Expression levels of β-catenin, TCF7, and c-Myc were detected via western blot analysis. Each experiment was performed in triplicate. Values presented represent the mean ± standard deviation of three independent experiments. *P

    Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Expressing, Western Blot, Standard Deviation

    Chemical structure of  β -Elemene.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Anti-Lung-Cancer Activity and Liposome-Based Delivery Systems of β-Elemene

    doi: 10.1155/2012/259523

    Figure Lengend Snippet: Chemical structure of β -Elemene.

    Article Snippet: Afterwards, an injection preparation containing 85% β -elemene developed by CSPC Pharmaceutical Limited Cooperation (China) has been approved by the SFDA, China, for the treatment of brain tumours and other carcinomas [ ].

    Techniques:

    β-Elemene blocks the cisplatin-induced increase in ERCC-1 protein in human ovarian tumor cells. A2780/CP70 cells were exposed to 40 μM cisplatin (CDDP) alone or cisplatin plus β-elemene (ELE) at the IC 20 for 24 or 48 h. The cells were harvested and lysed. Cell lysates containing 40 μg of protein were analyzed on western blots using ERCC-1 antiserum and antibody against β-actin (loading control), as described previously ( 23 , 24 ).

    Journal: International Journal of Oncology

    Article Title: ?-Elemene enhances susceptibility to cisplatin in resistant ovarian carcinoma cells via downregulation of ERCC-1 and XIAP and inactivation of JNK

    doi: 10.3892/ijo.2013.1996

    Figure Lengend Snippet: β-Elemene blocks the cisplatin-induced increase in ERCC-1 protein in human ovarian tumor cells. A2780/CP70 cells were exposed to 40 μM cisplatin (CDDP) alone or cisplatin plus β-elemene (ELE) at the IC 20 for 24 or 48 h. The cells were harvested and lysed. Cell lysates containing 40 μg of protein were analyzed on western blots using ERCC-1 antiserum and antibody against β-actin (loading control), as described previously ( 23 , 24 ).

    Article Snippet: Chemicals and immunoreagents The ()-β-elemene (98% purity) was obtained from Yuanda Pharmaceuticals Ltd. Inc. (Dalian, China).

    Techniques: Western Blot

    The chemical structure of β-elemene.

    Journal: International Journal of Oncology

    Article Title: ?-Elemene enhances susceptibility to cisplatin in resistant ovarian carcinoma cells via downregulation of ERCC-1 and XIAP and inactivation of JNK

    doi: 10.3892/ijo.2013.1996

    Figure Lengend Snippet: The chemical structure of β-elemene.

    Article Snippet: Chemicals and immunoreagents The ()-β-elemene (98% purity) was obtained from Yuanda Pharmaceuticals Ltd. Inc. (Dalian, China).

    Techniques:

    β-Elemene abrogates cisplatin-induced JNK1 protein phosphorylation in human ovarian tumor cells. A2780/CP70 cells were exposed to 40 μM cisplatin alone or cisplatin plus β-elemene (ELE) at the IC 20 for 1 or 3 h. The cells were harvested and lysed. Cell lysates containing 60 μg of protein were analyzed on western blots using antibodies against phospho-JNK1 (Thr183/Tyr185) and β-actin (loading control), as described previously ( 21 , 22 ).

    Journal: International Journal of Oncology

    Article Title: ?-Elemene enhances susceptibility to cisplatin in resistant ovarian carcinoma cells via downregulation of ERCC-1 and XIAP and inactivation of JNK

    doi: 10.3892/ijo.2013.1996

    Figure Lengend Snippet: β-Elemene abrogates cisplatin-induced JNK1 protein phosphorylation in human ovarian tumor cells. A2780/CP70 cells were exposed to 40 μM cisplatin alone or cisplatin plus β-elemene (ELE) at the IC 20 for 1 or 3 h. The cells were harvested and lysed. Cell lysates containing 60 μg of protein were analyzed on western blots using antibodies against phospho-JNK1 (Thr183/Tyr185) and β-actin (loading control), as described previously ( 21 , 22 ).

    Article Snippet: Chemicals and immunoreagents The ()-β-elemene (98% purity) was obtained from Yuanda Pharmaceuticals Ltd. Inc. (Dalian, China).

    Techniques: Western Blot

    β-Elemene downregulates XIAP protein levels in human ovarian tumor cells. A2780/CP70 cells were exposed to β-elemene at the indicated concentrations for 24 h. The cells were harvested and lysed. Cell lysates containing 60 μg of protein were analyzed on western blots using antibodies against XIAP and β-actin (loading control), as described previously ( 16 ).

    Journal: International Journal of Oncology

    Article Title: ?-Elemene enhances susceptibility to cisplatin in resistant ovarian carcinoma cells via downregulation of ERCC-1 and XIAP and inactivation of JNK

    doi: 10.3892/ijo.2013.1996

    Figure Lengend Snippet: β-Elemene downregulates XIAP protein levels in human ovarian tumor cells. A2780/CP70 cells were exposed to β-elemene at the indicated concentrations for 24 h. The cells were harvested and lysed. Cell lysates containing 60 μg of protein were analyzed on western blots using antibodies against XIAP and β-actin (loading control), as described previously ( 16 ).

    Article Snippet: Chemicals and immunoreagents The ()-β-elemene (98% purity) was obtained from Yuanda Pharmaceuticals Ltd. Inc. (Dalian, China).

    Techniques: Western Blot

    The effect of β-elemene and cisplatin on XIAP protein levels in human ovarian tumor cells. A2780/CP70 cells were exposed to 30 μM cisplatin (CDDP) alone, 70 μg/ml β-elemene alone, or cisplatin plus β-elemene (ELE) at the IC 20 for 12 or 24 h. The cells were harvested and lysed. Cell lysates containing 40 μg of protein were analyzed on western blots using antibodies against XIAP and β-actin (loading control).

    Journal: International Journal of Oncology

    Article Title: ?-Elemene enhances susceptibility to cisplatin in resistant ovarian carcinoma cells via downregulation of ERCC-1 and XIAP and inactivation of JNK

    doi: 10.3892/ijo.2013.1996

    Figure Lengend Snippet: The effect of β-elemene and cisplatin on XIAP protein levels in human ovarian tumor cells. A2780/CP70 cells were exposed to 30 μM cisplatin (CDDP) alone, 70 μg/ml β-elemene alone, or cisplatin plus β-elemene (ELE) at the IC 20 for 12 or 24 h. The cells were harvested and lysed. Cell lysates containing 40 μg of protein were analyzed on western blots using antibodies against XIAP and β-actin (loading control).

    Article Snippet: Chemicals and immunoreagents The ()-β-elemene (98% purity) was obtained from Yuanda Pharmaceuticals Ltd. Inc. (Dalian, China).

    Techniques: Western Blot

    Proposed molecular mechanism for the effect of β-elemene on cisplatin chemosensitivity in human ovarian carcinoma cells. In the proposed mechanism shown here, β-elemene mediates altered DNA repair activity and cisplatin sensitivity in human ovarian carcinoma cells by blocking cisplatin-induced PI3K/JNK and PI3K/Akt activation, thereby preventing from the activation of the downstream signaling components such as AP-1 and NF-κB. This leads to decreased DNA-repair activity and increased caspase activity, respectively, which both confer sensitization of resistant ovarian cancer cells to the chemotherapeutic agent cisplatin. See text for details.

    Journal: International Journal of Oncology

    Article Title: ?-Elemene enhances susceptibility to cisplatin in resistant ovarian carcinoma cells via downregulation of ERCC-1 and XIAP and inactivation of JNK

    doi: 10.3892/ijo.2013.1996

    Figure Lengend Snippet: Proposed molecular mechanism for the effect of β-elemene on cisplatin chemosensitivity in human ovarian carcinoma cells. In the proposed mechanism shown here, β-elemene mediates altered DNA repair activity and cisplatin sensitivity in human ovarian carcinoma cells by blocking cisplatin-induced PI3K/JNK and PI3K/Akt activation, thereby preventing from the activation of the downstream signaling components such as AP-1 and NF-κB. This leads to decreased DNA-repair activity and increased caspase activity, respectively, which both confer sensitization of resistant ovarian cancer cells to the chemotherapeutic agent cisplatin. See text for details.

    Article Snippet: Chemicals and immunoreagents The ()-β-elemene (98% purity) was obtained from Yuanda Pharmaceuticals Ltd. Inc. (Dalian, China).

    Techniques: Activity Assay, Blocking Assay, Activation Assay

    β-elemene reverse the resistance of HCT116p53 –/– cells to 5-Fu by inducing pro-death autophagy. (A) HCT116p53 +/+ and HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the protein expression of LC3B, Beclin-1, and Active caspase-3 were examined by Western blot; (B) HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the autophagy flow was observed by immunofluorescence, red arrow indicates autolysosomes; (C) HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the occurrence of autophagy was observed by transmission electron microscope; (D) HCT116p53 –/– cells were treated with 5-Fu + β-elemene, 5-Fu + β-elemene + Bafilomycin A1, the cell viability was examined by CCK8. (*** p

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: β-Elemene Reverses the Resistance of p53-Deficient Colorectal Cancer Cells to 5-Fluorouracil by Inducing Pro-death Autophagy and Cyclin D3-Dependent Cycle Arrest

    doi: 10.3389/fbioe.2020.00378

    Figure Lengend Snippet: β-elemene reverse the resistance of HCT116p53 –/– cells to 5-Fu by inducing pro-death autophagy. (A) HCT116p53 +/+ and HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the protein expression of LC3B, Beclin-1, and Active caspase-3 were examined by Western blot; (B) HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the autophagy flow was observed by immunofluorescence, red arrow indicates autolysosomes; (C) HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the occurrence of autophagy was observed by transmission electron microscope; (D) HCT116p53 –/– cells were treated with 5-Fu + β-elemene, 5-Fu + β-elemene + Bafilomycin A1, the cell viability was examined by CCK8. (*** p

    Article Snippet: Anti-rabbit IgG, HRP-linked Antibody (#7074) and Anti-mouse IgG, HRP-linked Antibody (#7076) were also obtained from CST. β-elemene was obtained from LKT lab (E4418, purity ≥98%), β-elemene was dissolved in ethanol (02483, Supelco).

    Techniques: Expressing, Western Blot, Immunofluorescence, Transmission Assay, Microscopy

    5-Fu and β-elemene inhibits proliferation of colorectal cancer cells. (A) HCT116p53 +/+ and HCT116p53 –/– cells were treated with different concentrations of 5-Fu for 24 h, or treated with β-elemene for 24, 48, and 72 h, the cell viability was examined by CCK8; (B) the cell proliferation was detected by a clone formation assays; (C) the apoptosis rate of cells was examined by flow cytometry. ( ∗ p

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: β-Elemene Reverses the Resistance of p53-Deficient Colorectal Cancer Cells to 5-Fluorouracil by Inducing Pro-death Autophagy and Cyclin D3-Dependent Cycle Arrest

    doi: 10.3389/fbioe.2020.00378

    Figure Lengend Snippet: 5-Fu and β-elemene inhibits proliferation of colorectal cancer cells. (A) HCT116p53 +/+ and HCT116p53 –/– cells were treated with different concentrations of 5-Fu for 24 h, or treated with β-elemene for 24, 48, and 72 h, the cell viability was examined by CCK8; (B) the cell proliferation was detected by a clone formation assays; (C) the apoptosis rate of cells was examined by flow cytometry. ( ∗ p

    Article Snippet: Anti-rabbit IgG, HRP-linked Antibody (#7074) and Anti-mouse IgG, HRP-linked Antibody (#7076) were also obtained from CST. β-elemene was obtained from LKT lab (E4418, purity ≥98%), β-elemene was dissolved in ethanol (02483, Supelco).

    Techniques: Flow Cytometry

    β-elemene reverse the resistance of HCT116p53 –/– cells to 5-Fu by inducing Cyclin D3- dependent cycle arrest. (A) HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the expression of cell cycle-related proteins were examined by Western blot; (B) HCT116p53 –/– cells were treated with 5-Fu + β-elemene, Cyclin-D3, 5-Fu + β-elemene + Cyclin D3, the protein expression of Cyclin D3 and CDK6 were examined by Western blot; (C) Overexpression Cyclin D3 of HCT116p53 –/– cells and treated with 5-Fu, β-elemene, 5-Fu + β-elemene, the cell viability was examined by CCK8. (** p

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: β-Elemene Reverses the Resistance of p53-Deficient Colorectal Cancer Cells to 5-Fluorouracil by Inducing Pro-death Autophagy and Cyclin D3-Dependent Cycle Arrest

    doi: 10.3389/fbioe.2020.00378

    Figure Lengend Snippet: β-elemene reverse the resistance of HCT116p53 –/– cells to 5-Fu by inducing Cyclin D3- dependent cycle arrest. (A) HCT116p53 –/– cells were treated with control, 5-Fu, β-elemene, 5-Fu + β-elemene, the expression of cell cycle-related proteins were examined by Western blot; (B) HCT116p53 –/– cells were treated with 5-Fu + β-elemene, Cyclin-D3, 5-Fu + β-elemene + Cyclin D3, the protein expression of Cyclin D3 and CDK6 were examined by Western blot; (C) Overexpression Cyclin D3 of HCT116p53 –/– cells and treated with 5-Fu, β-elemene, 5-Fu + β-elemene, the cell viability was examined by CCK8. (** p

    Article Snippet: Anti-rabbit IgG, HRP-linked Antibody (#7074) and Anti-mouse IgG, HRP-linked Antibody (#7076) were also obtained from CST. β-elemene was obtained from LKT lab (E4418, purity ≥98%), β-elemene was dissolved in ethanol (02483, Supelco).

    Techniques: Expressing, Western Blot, Over Expression

    Effect of β-elemene combined with 5-Fu on tumorigenicity of HCT116p53 –/– cells in vivo. (A) The tumor tissue; (B) Tumor volume curve. (* p

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: β-Elemene Reverses the Resistance of p53-Deficient Colorectal Cancer Cells to 5-Fluorouracil by Inducing Pro-death Autophagy and Cyclin D3-Dependent Cycle Arrest

    doi: 10.3389/fbioe.2020.00378

    Figure Lengend Snippet: Effect of β-elemene combined with 5-Fu on tumorigenicity of HCT116p53 –/– cells in vivo. (A) The tumor tissue; (B) Tumor volume curve. (* p

    Article Snippet: Anti-rabbit IgG, HRP-linked Antibody (#7074) and Anti-mouse IgG, HRP-linked Antibody (#7076) were also obtained from CST. β-elemene was obtained from LKT lab (E4418, purity ≥98%), β-elemene was dissolved in ethanol (02483, Supelco).

    Techniques: In Vivo

    The anti-proliferation and anti-migration effect assay in K30 cells when treated with β-elemene, DOX, dual drugs and bMED NPs: (A) Clony images of cells; (B) Quantitative data on the relative colony number; (C) The images of cells in transwell migration assay; (D) Quantitative data on transwell migration. Error bars represent the SD of the mean. * p

    Journal: Frontiers in Oncology

    Article Title: Construction of Biocompatible Dual-Drug Loaded Complicated Nanoparticles for in vivo Improvement of Synergistic Chemotherapy in Esophageal Cancer

    doi: 10.3389/fonc.2020.00622

    Figure Lengend Snippet: The anti-proliferation and anti-migration effect assay in K30 cells when treated with β-elemene, DOX, dual drugs and bMED NPs: (A) Clony images of cells; (B) Quantitative data on the relative colony number; (C) The images of cells in transwell migration assay; (D) Quantitative data on transwell migration. Error bars represent the SD of the mean. * p

    Article Snippet: Materials β-elemene were purchased from Sigma.

    Techniques: Migration, Transwell Migration Assay