α1 - acid Search Results


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  • 95
    Millipore α1 acid glycoprotein
    Computational algorithm of id-GPA for identification of standard <t>α1-acid</t> glycoprotein (AGP). id-GPA was designed for identification of N -glycopeptides in high-throughput analysis using three scoring systems (M-, S-, and Y-scores). ( a ) M-score for N- glycopeptide selection based on 15 glycan-specific oxonium ions from the total HCD-MS/MS spectra; N = 1,674, number of selected N -glycopeptide spectra for subsequent analysis with M-score > 1.3 from a total of 5,818 HCD spectra, at a false discovery rate (FDR) of 2.5% (as determined by manual validation). ( b ) S-score for the selection of N -glycopeptide candidates by matching the isotope distribution of N -glycopeptides in the database; n = 924, number of selected precursor ions of N- glycopeptide candidates with an S-score > 98.0 at an FDR of 19.7% (determined by manual validation). ( c ) Y-score for identification of N -glycopeptides by matching fragment ions in the CID and HCD spectra with the original 924 N -glycopeptide candidates. Ultimately, N = 456 N -glycopeptide spectra were identified with Y-score > 69.5 at an FDR of 0.0% (as determined by manual validation).
    α1 Acid Glycoprotein, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Glycoform alpha1 acid glycoprotein
    Computational algorithm of id-GPA for identification of standard <t>α1-acid</t> glycoprotein (AGP). id-GPA was designed for identification of N -glycopeptides in high-throughput analysis using three scoring systems (M-, S-, and Y-scores). ( a ) M-score for N- glycopeptide selection based on 15 glycan-specific oxonium ions from the total HCD-MS/MS spectra; N = 1,674, number of selected N -glycopeptide spectra for subsequent analysis with M-score > 1.3 from a total of 5,818 HCD spectra, at a false discovery rate (FDR) of 2.5% (as determined by manual validation). ( b ) S-score for the selection of N -glycopeptide candidates by matching the isotope distribution of N -glycopeptides in the database; n = 924, number of selected precursor ions of N- glycopeptide candidates with an S-score > 98.0 at an FDR of 19.7% (determined by manual validation). ( c ) Y-score for identification of N -glycopeptides by matching fragment ions in the CID and HCD spectra with the original 924 N -glycopeptide candidates. Ultimately, N = 456 N -glycopeptide spectra were identified with Y-score > 69.5 at an FDR of 0.0% (as determined by manual validation).
    Alpha1 Acid Glycoprotein, supplied by Glycoform, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore bovine α1 acid glycoprotein
    Computational algorithm of id-GPA for identification of standard <t>α1-acid</t> glycoprotein (AGP). id-GPA was designed for identification of N -glycopeptides in high-throughput analysis using three scoring systems (M-, S-, and Y-scores). ( a ) M-score for N- glycopeptide selection based on 15 glycan-specific oxonium ions from the total HCD-MS/MS spectra; N = 1,674, number of selected N -glycopeptide spectra for subsequent analysis with M-score > 1.3 from a total of 5,818 HCD spectra, at a false discovery rate (FDR) of 2.5% (as determined by manual validation). ( b ) S-score for the selection of N -glycopeptide candidates by matching the isotope distribution of N -glycopeptides in the database; n = 924, number of selected precursor ions of N- glycopeptide candidates with an S-score > 98.0 at an FDR of 19.7% (determined by manual validation). ( c ) Y-score for identification of N -glycopeptides by matching fragment ions in the CID and HCD spectra with the original 924 N -glycopeptide candidates. Ultimately, N = 456 N -glycopeptide spectra were identified with Y-score > 69.5 at an FDR of 0.0% (as determined by manual validation).
    Bovine α1 Acid Glycoprotein, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Glycoform α1 acid glycoprotein
    Computational algorithm of id-GPA for identification of standard <t>α1-acid</t> glycoprotein (AGP). id-GPA was designed for identification of N -glycopeptides in high-throughput analysis using three scoring systems (M-, S-, and Y-scores). ( a ) M-score for N- glycopeptide selection based on 15 glycan-specific oxonium ions from the total HCD-MS/MS spectra; N = 1,674, number of selected N -glycopeptide spectra for subsequent analysis with M-score > 1.3 from a total of 5,818 HCD spectra, at a false discovery rate (FDR) of 2.5% (as determined by manual validation). ( b ) S-score for the selection of N -glycopeptide candidates by matching the isotope distribution of N -glycopeptides in the database; n = 924, number of selected precursor ions of N- glycopeptide candidates with an S-score > 98.0 at an FDR of 19.7% (determined by manual validation). ( c ) Y-score for identification of N -glycopeptides by matching fragment ions in the CID and HCD spectra with the original 924 N -glycopeptide candidates. Ultimately, N = 456 N -glycopeptide spectra were identified with Y-score > 69.5 at an FDR of 0.0% (as determined by manual validation).
    α1 Acid Glycoprotein, supplied by Glycoform, used in various techniques. Bioz Stars score: 90/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α1 acid glycoprotein/product/Glycoform
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    88
    Siemens Healthineers human α1 acid glycoprotein
    Urinary excretion of fetuin-A, <t>α1-microglobulin,</t> orosomucoid and albumin creatinine ratio (ACR) in various stages of diabetic nephropathy (n = 85). All of the urinary excretion of sialylated glycoprpteins such as fetuin-A, α1-microglobulin, and orosomucoid are compared by Kruskal-Wallis test.
    Human α1 Acid Glycoprotein, supplied by Siemens Healthineers, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human α1 acid glycoprotein/product/Siemens Healthineers
    Average 88 stars, based on 16 article reviews
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    human α1 acid glycoprotein - by Bioz Stars, 2020-09
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    84
    Thermo Fisher captureselect biotin anti α1 acid glycoprotein conjugate
    Urinary excretion of fetuin-A, <t>α1-microglobulin,</t> orosomucoid and albumin creatinine ratio (ACR) in various stages of diabetic nephropathy (n = 85). All of the urinary excretion of sialylated glycoprpteins such as fetuin-A, α1-microglobulin, and orosomucoid are compared by Kruskal-Wallis test.
    Captureselect Biotin Anti α1 Acid Glycoprotein Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agrisera polyclonal rabbit antibody against α1 acid glycoprotein
    Urinary excretion of fetuin-A, <t>α1-microglobulin,</t> orosomucoid and albumin creatinine ratio (ACR) in various stages of diabetic nephropathy (n = 85). All of the urinary excretion of sialylated glycoprpteins such as fetuin-A, α1-microglobulin, and orosomucoid are compared by Kruskal-Wallis test.
    Polyclonal Rabbit Antibody Against α1 Acid Glycoprotein, supplied by Agrisera, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit antibody against α1 acid glycoprotein/product/Agrisera
    Average 85 stars, based on 12 article reviews
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    91
    Thermo Fisher alpha1 acid glycoprotein from human plasma
    Urinary excretion of fetuin-A, <t>α1-microglobulin,</t> orosomucoid and albumin creatinine ratio (ACR) in various stages of diabetic nephropathy (n = 85). All of the urinary excretion of sialylated glycoprpteins such as fetuin-A, α1-microglobulin, and orosomucoid are compared by Kruskal-Wallis test.
    Alpha1 Acid Glycoprotein From Human Plasma, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Abcam anti α1 syntrophin rabbit polyclonal antibody
    I mmunohistochemistry of dystrophin-associated proteins accompanied after 9 times i.v. injections of the 10-vPMO cocktail. Dystrophin, <t>α1-syntrophin,</t> nNOS, α-sarcoglycan, and β-dystroglycan were stained on serial sections of the muscles form nontreated and treated mdx52 mice. Biceps femoris muscles were shown as representative data in 3 treated mice. Asterisks indicate the same muscle fiber between the images. Scale bar, 100 μm.
    Anti α1 Syntrophin Rabbit Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Abcam α1 acid glycoprotein agp
    I mmunohistochemistry of dystrophin-associated proteins accompanied after 9 times i.v. injections of the 10-vPMO cocktail. Dystrophin, <t>α1-syntrophin,</t> nNOS, α-sarcoglycan, and β-dystroglycan were stained on serial sections of the muscles form nontreated and treated mdx52 mice. Biceps femoris muscles were shown as representative data in 3 treated mice. Asterisks indicate the same muscle fiber between the images. Scale bar, 100 μm.
    α1 Acid Glycoprotein Agp, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Simcyp α1 acid glycoprotein concentration
    I mmunohistochemistry of dystrophin-associated proteins accompanied after 9 times i.v. injections of the 10-vPMO cocktail. Dystrophin, <t>α1-syntrophin,</t> nNOS, α-sarcoglycan, and β-dystroglycan were stained on serial sections of the muscles form nontreated and treated mdx52 mice. Biceps femoris muscles were shown as representative data in 3 treated mice. Asterisks indicate the same muscle fiber between the images. Scale bar, 100 μm.
    α1 Acid Glycoprotein Concentration, supplied by Simcyp, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore α1 acid glycoprotein orosomucoid
    I mmunohistochemistry of dystrophin-associated proteins accompanied after 9 times i.v. injections of the 10-vPMO cocktail. Dystrophin, <t>α1-syntrophin,</t> nNOS, α-sarcoglycan, and β-dystroglycan were stained on serial sections of the muscles form nontreated and treated mdx52 mice. Biceps femoris muscles were shown as representative data in 3 treated mice. Asterisks indicate the same muscle fiber between the images. Scale bar, 100 μm.
    α1 Acid Glycoprotein Orosomucoid, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Computational algorithm of id-GPA for identification of standard α1-acid glycoprotein (AGP). id-GPA was designed for identification of N -glycopeptides in high-throughput analysis using three scoring systems (M-, S-, and Y-scores). ( a ) M-score for N- glycopeptide selection based on 15 glycan-specific oxonium ions from the total HCD-MS/MS spectra; N = 1,674, number of selected N -glycopeptide spectra for subsequent analysis with M-score > 1.3 from a total of 5,818 HCD spectra, at a false discovery rate (FDR) of 2.5% (as determined by manual validation). ( b ) S-score for the selection of N -glycopeptide candidates by matching the isotope distribution of N -glycopeptides in the database; n = 924, number of selected precursor ions of N- glycopeptide candidates with an S-score > 98.0 at an FDR of 19.7% (determined by manual validation). ( c ) Y-score for identification of N -glycopeptides by matching fragment ions in the CID and HCD spectra with the original 924 N -glycopeptide candidates. Ultimately, N = 456 N -glycopeptide spectra were identified with Y-score > 69.5 at an FDR of 0.0% (as determined by manual validation).

    Journal: Scientific Reports

    Article Title: Integrated GlycoProteome Analyzer (I-GPA) for Automated Identification and Quantitation of Site-Specific N-Glycosylation

    doi: 10.1038/srep21175

    Figure Lengend Snippet: Computational algorithm of id-GPA for identification of standard α1-acid glycoprotein (AGP). id-GPA was designed for identification of N -glycopeptides in high-throughput analysis using three scoring systems (M-, S-, and Y-scores). ( a ) M-score for N- glycopeptide selection based on 15 glycan-specific oxonium ions from the total HCD-MS/MS spectra; N = 1,674, number of selected N -glycopeptide spectra for subsequent analysis with M-score > 1.3 from a total of 5,818 HCD spectra, at a false discovery rate (FDR) of 2.5% (as determined by manual validation). ( b ) S-score for the selection of N -glycopeptide candidates by matching the isotope distribution of N -glycopeptides in the database; n = 924, number of selected precursor ions of N- glycopeptide candidates with an S-score > 98.0 at an FDR of 19.7% (determined by manual validation). ( c ) Y-score for identification of N -glycopeptides by matching fragment ions in the CID and HCD spectra with the original 924 N -glycopeptide candidates. Ultimately, N = 456 N -glycopeptide spectra were identified with Y-score > 69.5 at an FDR of 0.0% (as determined by manual validation).

    Article Snippet: Materials Glycoprotein standards (RNase B(source: bovine, Cat. No. R1153), α1-acid glycoprotein (source: human, Cat. No. G9885) and IgG (source: human, Cat. No. I4505), 1,4-dithiothreitol (DTT), iodoacetamide (IAA), trifluoroacetic acid (TFA) and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: High Throughput Screening Assay, Selection, Mass Spectrometry

    q-GPA algorithm for label-free quantitation of standard α1-acid glycoprotein (AGP). ( a ) The identified N- glycopeptides of AGP were quantitated based on the combined intensity of top three isotope peaks. For example, if we suppose to have two N- glycopeptides (#1 = CANLVPVPITNATLDQITGK_6503(+4) and #2 = CANLVPVPITNATLDQITGK_6522(+4)) with monoisotope mass of 1247.288 and 1247.533 Da, respectively, they might be distinguished in Fig. 3a (top) by MS due to their mass difference over 150 ppm. However, N- glycopeptide #2 in XIC (m/z = 1247.533) of Fig. 3a (bottom) can be interferenced by second isotope (m/z = 1247.538) of N- glycopeptide #1 due to their mass difference of 3–4 ppm. For correct quantification of N- glycopeptide #2, we introduced TIQ (Top Three Isotopes Quantification) method as shown in Fig. 3b,c, where 3TIQ uses the combined intensity of top three isotope peaks at three highest MS spectral points (Fig. 3b,c). Since we are evaluating the spectral pattern of selected MS spectra with S-score more than 98.0, it is possible to remove signal interference effectively from co-eluted peaks from similar m/z ions as shown in Fig. 3b, even though it has only mass difference of 3–4 ppm between the monoisotope ion of N- glycopeptide #2 and second isotope ion of N- glycopeptide #1. ( d ) Number of MS spectral points (1, 2, 3, 5, and 7) needed for TIQ, compared with the XIC manually extracted for quantitation. N is the number of selected N -glycopeptides used for quantitation of the AGP standard. ( e ) Gray bars indicate linear regression with XIC quantitation. Red line indicates the percentage of identified N -glycopeptides that were quantitated. The highest number of N -glycopeptides was quantitated by 3TIQ, which yielded the best linear regression (R 2 = 0.959) with XIC.

    Journal: Scientific Reports

    Article Title: Integrated GlycoProteome Analyzer (I-GPA) for Automated Identification and Quantitation of Site-Specific N-Glycosylation

    doi: 10.1038/srep21175

    Figure Lengend Snippet: q-GPA algorithm for label-free quantitation of standard α1-acid glycoprotein (AGP). ( a ) The identified N- glycopeptides of AGP were quantitated based on the combined intensity of top three isotope peaks. For example, if we suppose to have two N- glycopeptides (#1 = CANLVPVPITNATLDQITGK_6503(+4) and #2 = CANLVPVPITNATLDQITGK_6522(+4)) with monoisotope mass of 1247.288 and 1247.533 Da, respectively, they might be distinguished in Fig. 3a (top) by MS due to their mass difference over 150 ppm. However, N- glycopeptide #2 in XIC (m/z = 1247.533) of Fig. 3a (bottom) can be interferenced by second isotope (m/z = 1247.538) of N- glycopeptide #1 due to their mass difference of 3–4 ppm. For correct quantification of N- glycopeptide #2, we introduced TIQ (Top Three Isotopes Quantification) method as shown in Fig. 3b,c, where 3TIQ uses the combined intensity of top three isotope peaks at three highest MS spectral points (Fig. 3b,c). Since we are evaluating the spectral pattern of selected MS spectra with S-score more than 98.0, it is possible to remove signal interference effectively from co-eluted peaks from similar m/z ions as shown in Fig. 3b, even though it has only mass difference of 3–4 ppm between the monoisotope ion of N- glycopeptide #2 and second isotope ion of N- glycopeptide #1. ( d ) Number of MS spectral points (1, 2, 3, 5, and 7) needed for TIQ, compared with the XIC manually extracted for quantitation. N is the number of selected N -glycopeptides used for quantitation of the AGP standard. ( e ) Gray bars indicate linear regression with XIC quantitation. Red line indicates the percentage of identified N -glycopeptides that were quantitated. The highest number of N -glycopeptides was quantitated by 3TIQ, which yielded the best linear regression (R 2 = 0.959) with XIC.

    Article Snippet: Materials Glycoprotein standards (RNase B(source: bovine, Cat. No. R1153), α1-acid glycoprotein (source: human, Cat. No. G9885) and IgG (source: human, Cat. No. I4505), 1,4-dithiothreitol (DTT), iodoacetamide (IAA), trifluoroacetic acid (TFA) and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Quantitation Assay, Mass Spectrometry

    c-GPA algorithm for quantitation of three different HILIC-enriched batches of standard α1-acid glycoprotein (AGP). ( a ) Schematic algorithm of c-GPA for quantitation of N- glycopeptides from multiple samples. ( b ) Correlation coefficient of linear regressions (R 2 ) between areas of manual 3TIQ and XIC were above 0.93 for several N- glycopeptides from an AGP standard sample enriched by HILIC. ( c ) The Pearson correlation coefficient (r) of a scatter graph of the coefficients of variation (CVs) was the value r = 0.8199, indicating the similarity between the results obtained by 3TIQ and XIC. The correlation coefficient of the two methods had a p-value below 0.0001.

    Journal: Scientific Reports

    Article Title: Integrated GlycoProteome Analyzer (I-GPA) for Automated Identification and Quantitation of Site-Specific N-Glycosylation

    doi: 10.1038/srep21175

    Figure Lengend Snippet: c-GPA algorithm for quantitation of three different HILIC-enriched batches of standard α1-acid glycoprotein (AGP). ( a ) Schematic algorithm of c-GPA for quantitation of N- glycopeptides from multiple samples. ( b ) Correlation coefficient of linear regressions (R 2 ) between areas of manual 3TIQ and XIC were above 0.93 for several N- glycopeptides from an AGP standard sample enriched by HILIC. ( c ) The Pearson correlation coefficient (r) of a scatter graph of the coefficients of variation (CVs) was the value r = 0.8199, indicating the similarity between the results obtained by 3TIQ and XIC. The correlation coefficient of the two methods had a p-value below 0.0001.

    Article Snippet: Materials Glycoprotein standards (RNase B(source: bovine, Cat. No. R1153), α1-acid glycoprotein (source: human, Cat. No. G9885) and IgG (source: human, Cat. No. I4505), 1,4-dithiothreitol (DTT), iodoacetamide (IAA), trifluoroacetic acid (TFA) and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Quantitation Assay, Hydrophilic Interaction Liquid Chromatography

    Urinary excretion of fetuin-A, α1-microglobulin, orosomucoid and albumin creatinine ratio (ACR) in various stages of diabetic nephropathy (n = 85). All of the urinary excretion of sialylated glycoprpteins such as fetuin-A, α1-microglobulin, and orosomucoid are compared by Kruskal-Wallis test.

    Journal: PLoS ONE

    Article Title: Urinary Fetuin-A Is a Novel Marker for Diabetic Nephropathy in Type 2 Diabetes Identified by Lectin Microarray

    doi: 10.1371/journal.pone.0077118

    Figure Lengend Snippet: Urinary excretion of fetuin-A, α1-microglobulin, orosomucoid and albumin creatinine ratio (ACR) in various stages of diabetic nephropathy (n = 85). All of the urinary excretion of sialylated glycoprpteins such as fetuin-A, α1-microglobulin, and orosomucoid are compared by Kruskal-Wallis test.

    Article Snippet: Urinary excretions of fetuin-A, α1-microglobulin, and orosomucoid were measured with ELISA kit for Human Fetuin-A (BioVender, Modrice, Czech Republic), LZ Test Eiken α1-M (Eiken Chemical Co., Tokyo, Japan), and N Antiserum to Human α1-acid Glycoprotein (Siemens Healthcare Diagnostics Inc., Marburg, Germany).

    Techniques:

    Simple correlation of urinary excretion of fetuin-A, α1-microglobulin, orosomucoid with estimated glomerular filtration ratio (eGFR) and urinary albumin creatinine ratio (ACR) in the patients with diabetic nephropathy (n = 85). Spearman correlation coefficients are used to evaluate whether urinary levels of fetuin-A, α1-microglobulin, and orosomucoid correlate with eGFR and ACR.

    Journal: PLoS ONE

    Article Title: Urinary Fetuin-A Is a Novel Marker for Diabetic Nephropathy in Type 2 Diabetes Identified by Lectin Microarray

    doi: 10.1371/journal.pone.0077118

    Figure Lengend Snippet: Simple correlation of urinary excretion of fetuin-A, α1-microglobulin, orosomucoid with estimated glomerular filtration ratio (eGFR) and urinary albumin creatinine ratio (ACR) in the patients with diabetic nephropathy (n = 85). Spearman correlation coefficients are used to evaluate whether urinary levels of fetuin-A, α1-microglobulin, and orosomucoid correlate with eGFR and ACR.

    Article Snippet: Urinary excretions of fetuin-A, α1-microglobulin, and orosomucoid were measured with ELISA kit for Human Fetuin-A (BioVender, Modrice, Czech Republic), LZ Test Eiken α1-M (Eiken Chemical Co., Tokyo, Japan), and N Antiserum to Human α1-acid Glycoprotein (Siemens Healthcare Diagnostics Inc., Marburg, Germany).

    Techniques: Filtration

    I mmunohistochemistry of dystrophin-associated proteins accompanied after 9 times i.v. injections of the 10-vPMO cocktail. Dystrophin, α1-syntrophin, nNOS, α-sarcoglycan, and β-dystroglycan were stained on serial sections of the muscles form nontreated and treated mdx52 mice. Biceps femoris muscles were shown as representative data in 3 treated mice. Asterisks indicate the same muscle fiber between the images. Scale bar, 100 μm.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Long-Term Efficacy of Systemic Multiexon Skipping Targeting Dystrophin Exons 45–55 With a Cocktail of Vivo-Morpholinos in Mdx52 Mice

    doi: 10.1038/mtna.2014.76

    Figure Lengend Snippet: I mmunohistochemistry of dystrophin-associated proteins accompanied after 9 times i.v. injections of the 10-vPMO cocktail. Dystrophin, α1-syntrophin, nNOS, α-sarcoglycan, and β-dystroglycan were stained on serial sections of the muscles form nontreated and treated mdx52 mice. Biceps femoris muscles were shown as representative data in 3 treated mice. Asterisks indicate the same muscle fiber between the images. Scale bar, 100 μm.

    Article Snippet: In systemic treatment with the 10-vPMO cocktail, the diaphragm, biceps femoris, quadriceps, gastrocnemius, tibialis anterior, biceps brachii, triceps brachii, and heart muscles were examined 2 weeks after the final injection using anti-dystrophin (P7) antibody and antibodies against dystrophin-associated proteins: anti-α1-syntrophin rabbit polyclonal antibody (1:200, Abcam, Cambridge, UK), anti-nNOS rabbit polyclonal antibody (1:100, Invitrogen), anti-α-sarcoglycan mouse monoclonal antibody (1:10, Novocastra Laboratories), and anti-β-dystroglycan mouse monoclonal antibody (1:5, Novocastra Laboratories).

    Techniques: Staining, Mouse Assay