Journal: PLoS ONE
Article Title: Drought stress leads to systemic induced susceptibility to a nectrotrophic fungus associated with mountain pine beetle in Pinus banksiana seedlings
Figure Lengend Snippet: Effect of induction and watering treatments on the profile of monoterpene accumulations in bark of Pinus banksiana seedlings (A) at the site of the initial induction treatment application (i.e., the lower third of tree for control, methyl jasmonate and methyl salicylate treated trees and lesion tissue where Grosmannia clavigera was inoculated) and (B) above the induction treatment. Non-metric multidimensional scaling with Bray-Curtis distance ordination was used to analyze relationships. Significant monoterpene compounds are represented by overlaid vectors with direction indicating association with corresponding induction and watering treatments (α = 0.05; see S2 Table for correlations and P -values). Longer vectors show stronger correlations with the ordination configuration (i.e., axes 1 and 2). The minimum stress was: (A) 0.04 and (B) 0.17. Abbreviations for monoterpenes: αP = α-pinene, CM = camphene, βP = β-pinene, 3C = 3-carene, MY = myrcene, αT = α-terpinene, LM = limonene, βL = β-phellandrene, γT = γ-terpinene, CY = p -cymene, TR = terpinolene, CP = camphor, BA = bornyl acetate, and 4A = 4-allylanisole.
Article Snippet: To quantify individual and total compounds (ng mg-1 of fresh tissue, hereafter concentration), the following 14 standards were used: α-terpinene, γ-terpinene (Sigma-Aldrich, St. Louis, MO, USA), 3-carene, terpinolene, α-pinene, β-pinene, limonene, myrcene, camphene, p -cymene, 4-allylanisole (Fluka, Sigma-Aldrich, Buchs, Switzerland), bornyl acetate (SAFC Supply Solutions, St. Louis, MO, USA), and β-phellandrene (Glidco Inc., Jacksonville, FL, USA).