Article Title: Do age and mating status affect olfactory response of the parasitoid,Microplitis croceipes (Hymenoptera: Braconidae) to host-related plant odors?
Figure Lengend Snippet: Effect of age and mating status on the attraction of female Microplitis croceipes to four host-related plant odors in Y-tube olfactometer bioassays. Bars represent percentage of mated and unmated parasitoids of ages 1–3, 4–6, and 7–9 d-old when given a choice between hexane (solvent control) and synthetic compounds cis -3-hexenol ( A ), α-pinene ( B ), a 50/50 v/v binary mixture of cis -3-hexenol and α-pinene ( C ), and a choice between control jar (with no plant) and Heliothis virescens -infested cotton ( D ). Synthetic compounds were formulated in hexane at 1 µg/µl and presented as 10 µl samples (10 µg dose). Thirty 2 nd –3 rd instar larvae of H. virescens were allowed to infest cotton plants for 24 h before bioassays. N = 20 responding parasitoids per choice test. Numbers in the bars indicate actual number of responding individuals that chose each arm of the olfactometer. Asterisk (*) indicates significant deviation of parasitoid responses from a 50:50 (stimulus: control) distribution ( χ 2 goodness of fit test, P
Article Snippet: The number of non-responding parasitoids in the 24 sets of bioassays ranged from 0 to 5 with a mean of 1.2 insects per test. cis -3-Hexenol (CAT#: W256307) and α-pinene (CAT#: 147524) (purity 95-99%; Sigma-Aldrich®, St. Louis, MO, USA) were individually formulated in hexane (HPLC-grade) at 1 µg/µl concentration.