α-mem medium Search Results


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  • 90
    Thermo Fisher α mem medium
    The effect of resveratrol on TNF-α-induced cell injury in MC3T3-E1 cells. Cells were treated with <t>α-MEM</t> (control), TNF-α (5 ng/mL), TNF-α+Resveratrol (10 μmol/L, a Sirt1 activator), TNF-α+Resveratrol+EX-527
    α Mem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare α mem medium
    The effect of resveratrol on TNF-α-induced cell injury in MC3T3-E1 cells. Cells were treated with <t>α-MEM</t> (control), TNF-α (5 ng/mL), TNF-α+Resveratrol (10 μmol/L, a Sirt1 activator), TNF-α+Resveratrol+EX-527
    α Mem Medium, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore α mem medium
    The number of TRACP+ multinuclear cells . Mouse (C57BL/6, 8–12 weeks) bone marrow cells were isolated from mouse long bones and seeded in 24-well plates on bovine bone slices. Cells on the bone slices were cultured in four groups: control, VEGF (100 ng/ml) and ENDO (2 μg/ml) and VEGF + ENDO respectively. The control group was cultured in <t>α-MEM</t> with 10% FCS containing 30 ng/ml RANKL and 10 ng/ml M-CSF for 7 days, after which the cultures were stopped by fixing the cells with 3% paraformaldehyde (PFA)/2% sucrose in PBS.
    α Mem Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 466 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PAN - Biotech α mem medium
    Supernatant conditioned-media from h-iPSCs ( h-iPSC CM) is osteoinductive. ( A ) Numbers of h-MSC per field which had migrated after 6 hours of exposure to h-iPSC CM. Control(−) = <t>α-MEM</t> only; Control(+) = α-MEM + 10% FBS; h-iPSC CM = α-MEM: h-iPSC CM (1:2). ( B ) Time course of h-MSC proliferation in the presence of h-iPSC CM (Control(+) is significantly higher than the two others conditions at day 4 ($) and day 7 (*), n = 3; p
    α Mem Medium, supplied by PAN - Biotech, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech α mem medium
    Supernatant conditioned-media from h-iPSCs ( h-iPSC CM) is osteoinductive. ( A ) Numbers of h-MSC per field which had migrated after 6 hours of exposure to h-iPSC CM. Control(−) = <t>α-MEM</t> only; Control(+) = α-MEM + 10% FBS; h-iPSC CM = α-MEM: h-iPSC CM (1:2). ( B ) Time course of h-MSC proliferation in the presence of h-iPSC CM (Control(+) is significantly higher than the two others conditions at day 4 ($) and day 7 (*), n = 3; p
    α Mem Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biochrom α mem medium
    Supernatant conditioned-media from h-iPSCs ( h-iPSC CM) is osteoinductive. ( A ) Numbers of h-MSC per field which had migrated after 6 hours of exposure to h-iPSC CM. Control(−) = <t>α-MEM</t> only; Control(+) = α-MEM + 10% FBS; h-iPSC CM = α-MEM: h-iPSC CM (1:2). ( B ) Time course of h-MSC proliferation in the presence of h-iPSC CM (Control(+) is significantly higher than the two others conditions at day 4 ($) and day 7 (*), n = 3; p
    α Mem Medium, supplied by Biochrom, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lonza α mem medium
    Supernatant conditioned-media from h-iPSCs ( h-iPSC CM) is osteoinductive. ( A ) Numbers of h-MSC per field which had migrated after 6 hours of exposure to h-iPSC CM. Control(−) = <t>α-MEM</t> only; Control(+) = α-MEM + 10% FBS; h-iPSC CM = α-MEM: h-iPSC CM (1:2). ( B ) Time course of h-MSC proliferation in the presence of h-iPSC CM (Control(+) is significantly higher than the two others conditions at day 4 ($) and day 7 (*), n = 3; p
    α Mem Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FUJIFILM α mem medium
    Supernatant conditioned-media from h-iPSCs ( h-iPSC CM) is osteoinductive. ( A ) Numbers of h-MSC per field which had migrated after 6 hours of exposure to h-iPSC CM. Control(−) = <t>α-MEM</t> only; Control(+) = α-MEM + 10% FBS; h-iPSC CM = α-MEM: h-iPSC CM (1:2). ( B ) Time course of h-MSC proliferation in the presence of h-iPSC CM (Control(+) is significantly higher than the two others conditions at day 4 ($) and day 7 (*), n = 3; p
    α Mem Medium, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biological Industries Inc α mem medium
    ROS formation occurs partially downstream of class III PI3K activation. ( A ) Left panel: HeLa cells were starved for 2 h in the presence or absence of either 100 nM wortmannin or 10 mM 3MA, after which they were treated with DCFDA and analyzed by a fluorimeter as described above. Right panel: the rate of degradation of long-lived proteins was measured in cells incubated in either <t>α-MEM</t> medium or EBSS medium in the absence or presence of 100 nM wortmannin or 10 mM 3MA. Values are represented as the means±s.d. of three separate determinations. ( B ) WT MEFs or Atg5 (−/−) MEFs from two separate clones were starved for 2 h before treatment with DCFDA and visualization or fluorometric analysis.
    α Mem Medium, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Valiant α mem medium
    ROS formation occurs partially downstream of class III PI3K activation. ( A ) Left panel: HeLa cells were starved for 2 h in the presence or absence of either 100 nM wortmannin or 10 mM 3MA, after which they were treated with DCFDA and analyzed by a fluorimeter as described above. Right panel: the rate of degradation of long-lived proteins was measured in cells incubated in either <t>α-MEM</t> medium or EBSS medium in the absence or presence of 100 nM wortmannin or 10 mM 3MA. Values are represented as the means±s.d. of three separate determinations. ( B ) WT MEFs or Atg5 (−/−) MEFs from two separate clones were starved for 2 h before treatment with DCFDA and visualization or fluorometric analysis.
    α Mem Medium, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Welgene inc α mem medium
    ROS formation occurs partially downstream of class III PI3K activation. ( A ) Left panel: HeLa cells were starved for 2 h in the presence or absence of either 100 nM wortmannin or 10 mM 3MA, after which they were treated with DCFDA and analyzed by a fluorimeter as described above. Right panel: the rate of degradation of long-lived proteins was measured in cells incubated in either <t>α-MEM</t> medium or EBSS medium in the absence or presence of 100 nM wortmannin or 10 mM 3MA. Values are represented as the means±s.d. of three separate determinations. ( B ) WT MEFs or Atg5 (−/−) MEFs from two separate clones were starved for 2 h before treatment with DCFDA and visualization or fluorometric analysis.
    α Mem Medium, supplied by Welgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai α mem medium
    ROS formation occurs partially downstream of class III PI3K activation. ( A ) Left panel: HeLa cells were starved for 2 h in the presence or absence of either 100 nM wortmannin or 10 mM 3MA, after which they were treated with DCFDA and analyzed by a fluorimeter as described above. Right panel: the rate of degradation of long-lived proteins was measured in cells incubated in either <t>α-MEM</t> medium or EBSS medium in the absence or presence of 100 nM wortmannin or 10 mM 3MA. Values are represented as the means±s.d. of three separate determinations. ( B ) WT MEFs or Atg5 (−/−) MEFs from two separate clones were starved for 2 h before treatment with DCFDA and visualization or fluorometric analysis.
    α Mem Medium, supplied by Nacalai, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher α modified eagle medium αmem
    ROS formation occurs partially downstream of class III PI3K activation. ( A ) Left panel: HeLa cells were starved for 2 h in the presence or absence of either 100 nM wortmannin or 10 mM 3MA, after which they were treated with DCFDA and analyzed by a fluorimeter as described above. Right panel: the rate of degradation of long-lived proteins was measured in cells incubated in either <t>α-MEM</t> medium or EBSS medium in the absence or presence of 100 nM wortmannin or 10 mM 3MA. Values are represented as the means±s.d. of three separate determinations. ( B ) WT MEFs or Atg5 (−/−) MEFs from two separate clones were starved for 2 h before treatment with DCFDA and visualization or fluorometric analysis.
    α Modified Eagle Medium αmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare α modified eagle medium α mem
    ROS formation occurs partially downstream of class III PI3K activation. ( A ) Left panel: HeLa cells were starved for 2 h in the presence or absence of either 100 nM wortmannin or 10 mM 3MA, after which they were treated with DCFDA and analyzed by a fluorimeter as described above. Right panel: the rate of degradation of long-lived proteins was measured in cells incubated in either <t>α-MEM</t> medium or EBSS medium in the absence or presence of 100 nM wortmannin or 10 mM 3MA. Values are represented as the means±s.d. of three separate determinations. ( B ) WT MEFs or Atg5 (−/−) MEFs from two separate clones were starved for 2 h before treatment with DCFDA and visualization or fluorometric analysis.
    α Modified Eagle Medium α Mem, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Corning Life Sciences α mem medium
    ROS formation occurs partially downstream of class III PI3K activation. ( A ) Left panel: HeLa cells were starved for 2 h in the presence or absence of either 100 nM wortmannin or 10 mM 3MA, after which they were treated with DCFDA and analyzed by a fluorimeter as described above. Right panel: the rate of degradation of long-lived proteins was measured in cells incubated in either <t>α-MEM</t> medium or EBSS medium in the absence or presence of 100 nM wortmannin or 10 mM 3MA. Values are represented as the means±s.d. of three separate determinations. ( B ) WT MEFs or Atg5 (−/−) MEFs from two separate clones were starved for 2 h before treatment with DCFDA and visualization or fluorometric analysis.
    α Mem Medium, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Fisher Scientific α mem medium
    Humoral and cellular immune responses induced by different schemes of vaccination with Δ Likh1 . Humoral immune response was evaluated by conventional ELISA measurement of serum levels of different isotypes of Li WT SLA-specific antibodies (IgG total , IgG 1 and IgG 2a ). ( a ) Seroconversion of mice 20 days post-immunization with Δ Likh1 . ( b ) IgG2a:IgG1 ratio between IV and SC prime-boosted groups. ( c – h ) Levels of cytokines in pg/mL in the supernatant of Li WT SLA-stimulated splenocytes. The concentration of Li WT SLA in the experiments was 25 μg/mL and splenocytes were stimulated for 72 h. CpG ODN at 5 μg/mL was used as a positive control of a Th1-response and <t>α-MEM</t> medium with 20% of FBS was used as negative control. Cytokines were measured by flow cytometry with the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA, USA) following manufacturer instructions. The acquisition and data analyses were made in BD FACSVerse Instrument and FCAP Array Software v3. The detection limit of IL-2, IL-4, IL-6, IFN-γ, TNF, IL-17A and IL-10 cytokines were respectively 0.1; 0.03; 1.4; 0.5; 0.9; 0.8 and 16.8 pg/mL as well as cytokines secreted by Li WT SLA-stimulated splenocytes from immunized mice. Data are shown as means ± SE. Statistical analyses used One-way-ANOVA followed by Bonferroni’s multiple comparison test. *** p
    α Mem Medium, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellgro α mem medium
    Humoral and cellular immune responses induced by different schemes of vaccination with Δ Likh1 . Humoral immune response was evaluated by conventional ELISA measurement of serum levels of different isotypes of Li WT SLA-specific antibodies (IgG total , IgG 1 and IgG 2a ). ( a ) Seroconversion of mice 20 days post-immunization with Δ Likh1 . ( b ) IgG2a:IgG1 ratio between IV and SC prime-boosted groups. ( c – h ) Levels of cytokines in pg/mL in the supernatant of Li WT SLA-stimulated splenocytes. The concentration of Li WT SLA in the experiments was 25 μg/mL and splenocytes were stimulated for 72 h. CpG ODN at 5 μg/mL was used as a positive control of a Th1-response and <t>α-MEM</t> medium with 20% of FBS was used as negative control. Cytokines were measured by flow cytometry with the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA, USA) following manufacturer instructions. The acquisition and data analyses were made in BD FACSVerse Instrument and FCAP Array Software v3. The detection limit of IL-2, IL-4, IL-6, IFN-γ, TNF, IL-17A and IL-10 cytokines were respectively 0.1; 0.03; 1.4; 0.5; 0.9; 0.8 and 16.8 pg/mL as well as cytokines secreted by Li WT SLA-stimulated splenocytes from immunized mice. Data are shown as means ± SE. Statistical analyses used One-way-ANOVA followed by Bonferroni’s multiple comparison test. *** p
    α Mem Medium, supplied by Cellgro, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher serum free α mem medium
    Humoral and cellular immune responses induced by different schemes of vaccination with Δ Likh1 . Humoral immune response was evaluated by conventional ELISA measurement of serum levels of different isotypes of Li WT SLA-specific antibodies (IgG total , IgG 1 and IgG 2a ). ( a ) Seroconversion of mice 20 days post-immunization with Δ Likh1 . ( b ) IgG2a:IgG1 ratio between IV and SC prime-boosted groups. ( c – h ) Levels of cytokines in pg/mL in the supernatant of Li WT SLA-stimulated splenocytes. The concentration of Li WT SLA in the experiments was 25 μg/mL and splenocytes were stimulated for 72 h. CpG ODN at 5 μg/mL was used as a positive control of a Th1-response and <t>α-MEM</t> medium with 20% of FBS was used as negative control. Cytokines were measured by flow cytometry with the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA, USA) following manufacturer instructions. The acquisition and data analyses were made in BD FACSVerse Instrument and FCAP Array Software v3. The detection limit of IL-2, IL-4, IL-6, IFN-γ, TNF, IL-17A and IL-10 cytokines were respectively 0.1; 0.03; 1.4; 0.5; 0.9; 0.8 and 16.8 pg/mL as well as cytokines secreted by Li WT SLA-stimulated splenocytes from immunized mice. Data are shown as means ± SE. Statistical analyses used One-way-ANOVA followed by Bonferroni’s multiple comparison test. *** p
    Serum Free α Mem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    EuroClone α mem medium
    Humoral and cellular immune responses induced by different schemes of vaccination with Δ Likh1 . Humoral immune response was evaluated by conventional ELISA measurement of serum levels of different isotypes of Li WT SLA-specific antibodies (IgG total , IgG 1 and IgG 2a ). ( a ) Seroconversion of mice 20 days post-immunization with Δ Likh1 . ( b ) IgG2a:IgG1 ratio between IV and SC prime-boosted groups. ( c – h ) Levels of cytokines in pg/mL in the supernatant of Li WT SLA-stimulated splenocytes. The concentration of Li WT SLA in the experiments was 25 μg/mL and splenocytes were stimulated for 72 h. CpG ODN at 5 μg/mL was used as a positive control of a Th1-response and <t>α-MEM</t> medium with 20% of FBS was used as negative control. Cytokines were measured by flow cytometry with the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA, USA) following manufacturer instructions. The acquisition and data analyses were made in BD FACSVerse Instrument and FCAP Array Software v3. The detection limit of IL-2, IL-4, IL-6, IFN-γ, TNF, IL-17A and IL-10 cytokines were respectively 0.1; 0.03; 1.4; 0.5; 0.9; 0.8 and 16.8 pg/mL as well as cytokines secreted by Li WT SLA-stimulated splenocytes from immunized mice. Data are shown as means ± SE. Statistical analyses used One-way-ANOVA followed by Bonferroni’s multiple comparison test. *** p
    α Mem Medium, supplied by EuroClone, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cayman Chemical α mem medium
    C3aR/C5aR are required for efficient OC differentiation . (A) 2 × 10 6 WT, C3aR −/− , C5aR −/− , and C3aR −/− C5aR −/− BM cells were cultured in <t>α-MEM/10%</t> heat-inactivated media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 . OCs were stained, and TRAP-positive cells in each well were counted under a microscope. (B) WT BM cells (2 × 10 6 ) were cultured in α-MEM/10% heat-inactivated FBS media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 in the presence of placebo (control), C3aRA, C5aRA, or C3aRA·C5aRA. OCs were stained, and TRAP-positive cells in each well were counted under a microscope. Results are representative from 2 independent experiments performed in triplicate. Data are mean ± SD * P
    α Mem Medium, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Wisent Corporation α mem medium
    C3aR/C5aR are required for efficient OC differentiation . (A) 2 × 10 6 WT, C3aR −/− , C5aR −/− , and C3aR −/− C5aR −/− BM cells were cultured in <t>α-MEM/10%</t> heat-inactivated media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 . OCs were stained, and TRAP-positive cells in each well were counted under a microscope. (B) WT BM cells (2 × 10 6 ) were cultured in α-MEM/10% heat-inactivated FBS media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 in the presence of placebo (control), C3aRA, C5aRA, or C3aRA·C5aRA. OCs were stained, and TRAP-positive cells in each well were counted under a microscope. Results are representative from 2 independent experiments performed in triplicate. Data are mean ± SD * P
    α Mem Medium, supplied by Wisent Corporation, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Atlanta Biologicals α mem medium
    C3aR/C5aR are required for efficient OC differentiation . (A) 2 × 10 6 WT, C3aR −/− , C5aR −/− , and C3aR −/− C5aR −/− BM cells were cultured in <t>α-MEM/10%</t> heat-inactivated media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 . OCs were stained, and TRAP-positive cells in each well were counted under a microscope. (B) WT BM cells (2 × 10 6 ) were cultured in α-MEM/10% heat-inactivated FBS media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 in the presence of placebo (control), C3aRA, C5aRA, or C3aRA·C5aRA. OCs were stained, and TRAP-positive cells in each well were counted under a microscope. Results are representative from 2 independent experiments performed in triplicate. Data are mean ± SD * P
    α Mem Medium, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The effect of resveratrol on TNF-α-induced cell injury in MC3T3-E1 cells. Cells were treated with α-MEM (control), TNF-α (5 ng/mL), TNF-α+Resveratrol (10 μmol/L, a Sirt1 activator), TNF-α+Resveratrol+EX-527

    Journal: Acta Pharmacologica Sinica

    Article Title: Sirt1 overexpression protects murine osteoblasts against TNF-α-induced injury in vitro by suppressing the NF-κB signaling pathway

    doi: 10.1038/aps.2011.189

    Figure Lengend Snippet: The effect of resveratrol on TNF-α-induced cell injury in MC3T3-E1 cells. Cells were treated with α-MEM (control), TNF-α (5 ng/mL), TNF-α+Resveratrol (10 μmol/L, a Sirt1 activator), TNF-α+Resveratrol+EX-527

    Article Snippet: The murine osteoblastic cell line, MC3T3-E1, was cultured at 37 °C in 5% CO2 and 90% humidity in α-MEM medium (Invitrogen, Paisley, UK) supplemented with 10% fetal bovine serum and 100 μg/mL gentamicin (Invitrogen, Life Technologies, Paisley, Scotland).

    Techniques:

    The number of TRACP+ multinuclear cells . Mouse (C57BL/6, 8–12 weeks) bone marrow cells were isolated from mouse long bones and seeded in 24-well plates on bovine bone slices. Cells on the bone slices were cultured in four groups: control, VEGF (100 ng/ml) and ENDO (2 μg/ml) and VEGF + ENDO respectively. The control group was cultured in α-MEM with 10% FCS containing 30 ng/ml RANKL and 10 ng/ml M-CSF for 7 days, after which the cultures were stopped by fixing the cells with 3% paraformaldehyde (PFA)/2% sucrose in PBS.

    Journal: BMC Musculoskeletal Disorders

    Article Title: Endostatin inhibits VEGF-A induced osteoclastic bone resorption in vitro

    doi: 10.1186/1471-2474-7-56

    Figure Lengend Snippet: The number of TRACP+ multinuclear cells . Mouse (C57BL/6, 8–12 weeks) bone marrow cells were isolated from mouse long bones and seeded in 24-well plates on bovine bone slices. Cells on the bone slices were cultured in four groups: control, VEGF (100 ng/ml) and ENDO (2 μg/ml) and VEGF + ENDO respectively. The control group was cultured in α-MEM with 10% FCS containing 30 ng/ml RANKL and 10 ng/ml M-CSF for 7 days, after which the cultures were stopped by fixing the cells with 3% paraformaldehyde (PFA)/2% sucrose in PBS.

    Article Snippet: The control group was cultured in α-MEM medium (Sigma, UK) with 10% heat-inactivated fetal calf serum (FCS), 2 mM L-glutamin, 100 IU/ml penicillin, 10 μg/ml streptomycin and 20 mM Hepes which medium will be later referred to as α-MEM-FCS.

    Techniques: Isolation, Cell Culture

    Supernatant conditioned-media from h-iPSCs ( h-iPSC CM) is osteoinductive. ( A ) Numbers of h-MSC per field which had migrated after 6 hours of exposure to h-iPSC CM. Control(−) = α-MEM only; Control(+) = α-MEM + 10% FBS; h-iPSC CM = α-MEM: h-iPSC CM (1:2). ( B ) Time course of h-MSC proliferation in the presence of h-iPSC CM (Control(+) is significantly higher than the two others conditions at day 4 ($) and day 7 (*), n = 3; p

    Journal: Scientific Reports

    Article Title: The paracrine effects of human induced pluripotent stem cells promote bone-like structures via the upregulation of BMP expression in a mouse ectopic model

    doi: 10.1038/s41598-018-35546-6

    Figure Lengend Snippet: Supernatant conditioned-media from h-iPSCs ( h-iPSC CM) is osteoinductive. ( A ) Numbers of h-MSC per field which had migrated after 6 hours of exposure to h-iPSC CM. Control(−) = α-MEM only; Control(+) = α-MEM + 10% FBS; h-iPSC CM = α-MEM: h-iPSC CM (1:2). ( B ) Time course of h-MSC proliferation in the presence of h-iPSC CM (Control(+) is significantly higher than the two others conditions at day 4 ($) and day 7 (*), n = 3; p

    Article Snippet: h-iPSCs and h-MSCs (used as control) were seeded at 25.000 cells/cm2 in 24-well cell-culture plasticware and were cultured in α-MEM medium (PAN Biotech; USA) containing 5 g/L glucose without any added serum in a humidified, 37 °C, 5% CO2 , 95% N2 and 0.1% oxygen environment for 3 days.

    Techniques:

    ROS formation occurs partially downstream of class III PI3K activation. ( A ) Left panel: HeLa cells were starved for 2 h in the presence or absence of either 100 nM wortmannin or 10 mM 3MA, after which they were treated with DCFDA and analyzed by a fluorimeter as described above. Right panel: the rate of degradation of long-lived proteins was measured in cells incubated in either α-MEM medium or EBSS medium in the absence or presence of 100 nM wortmannin or 10 mM 3MA. Values are represented as the means±s.d. of three separate determinations. ( B ) WT MEFs or Atg5 (−/−) MEFs from two separate clones were starved for 2 h before treatment with DCFDA and visualization or fluorometric analysis.

    Journal: The EMBO Journal

    Article Title: Reactive oxygen species are essential for autophagy and specifically regulate the activity of Atg4

    doi: 10.1038/sj.emboj.7601623

    Figure Lengend Snippet: ROS formation occurs partially downstream of class III PI3K activation. ( A ) Left panel: HeLa cells were starved for 2 h in the presence or absence of either 100 nM wortmannin or 10 mM 3MA, after which they were treated with DCFDA and analyzed by a fluorimeter as described above. Right panel: the rate of degradation of long-lived proteins was measured in cells incubated in either α-MEM medium or EBSS medium in the absence or presence of 100 nM wortmannin or 10 mM 3MA. Values are represented as the means±s.d. of three separate determinations. ( B ) WT MEFs or Atg5 (−/−) MEFs from two separate clones were starved for 2 h before treatment with DCFDA and visualization or fluorometric analysis.

    Article Snippet: All cell lines were grown in α-MEM medium (Biological Industries, Israel) supplemented with 10% fetal calf serum and referred to as control medium.

    Techniques: Activation Assay, Incubation, Clone Assay

    Cells accumulate ROS under starvation conditions. ( A ) CHO cells were grown in a control medium (see Materials and methods) or starved for 3 or 13 h, after which they were incubated in 50 μM DHE and visualized as detailed in Materials and methods. ( B ) CHO cells were grown as in (A), treated with 5 nM MitoTracker Red for 30 min at 37°C, washed, treated with 30 μM DCFDA and visualized as detailed in Materials and methods. ( C ) HeLa cells grown in 96-well plates were deprived of serum (αMEM), completely starved (EBSS) or maintained in a control medium (α-MEM, 10% FCS) for 2 h, after which they were treated with DCFDA as in (B) and subsequently analyzed in a fluorimeter, as explained in Materials and methods. ( D ) Data collected from the fluorometric measurements were analyzed as detailed in Materials and methods.

    Journal: The EMBO Journal

    Article Title: Reactive oxygen species are essential for autophagy and specifically regulate the activity of Atg4

    doi: 10.1038/sj.emboj.7601623

    Figure Lengend Snippet: Cells accumulate ROS under starvation conditions. ( A ) CHO cells were grown in a control medium (see Materials and methods) or starved for 3 or 13 h, after which they were incubated in 50 μM DHE and visualized as detailed in Materials and methods. ( B ) CHO cells were grown as in (A), treated with 5 nM MitoTracker Red for 30 min at 37°C, washed, treated with 30 μM DCFDA and visualized as detailed in Materials and methods. ( C ) HeLa cells grown in 96-well plates were deprived of serum (αMEM), completely starved (EBSS) or maintained in a control medium (α-MEM, 10% FCS) for 2 h, after which they were treated with DCFDA as in (B) and subsequently analyzed in a fluorimeter, as explained in Materials and methods. ( D ) Data collected from the fluorometric measurements were analyzed as detailed in Materials and methods.

    Article Snippet: All cell lines were grown in α-MEM medium (Biological Industries, Israel) supplemented with 10% fetal calf serum and referred to as control medium.

    Techniques: Incubation

    Humoral and cellular immune responses induced by different schemes of vaccination with Δ Likh1 . Humoral immune response was evaluated by conventional ELISA measurement of serum levels of different isotypes of Li WT SLA-specific antibodies (IgG total , IgG 1 and IgG 2a ). ( a ) Seroconversion of mice 20 days post-immunization with Δ Likh1 . ( b ) IgG2a:IgG1 ratio between IV and SC prime-boosted groups. ( c – h ) Levels of cytokines in pg/mL in the supernatant of Li WT SLA-stimulated splenocytes. The concentration of Li WT SLA in the experiments was 25 μg/mL and splenocytes were stimulated for 72 h. CpG ODN at 5 μg/mL was used as a positive control of a Th1-response and α-MEM medium with 20% of FBS was used as negative control. Cytokines were measured by flow cytometry with the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA, USA) following manufacturer instructions. The acquisition and data analyses were made in BD FACSVerse Instrument and FCAP Array Software v3. The detection limit of IL-2, IL-4, IL-6, IFN-γ, TNF, IL-17A and IL-10 cytokines were respectively 0.1; 0.03; 1.4; 0.5; 0.9; 0.8 and 16.8 pg/mL as well as cytokines secreted by Li WT SLA-stimulated splenocytes from immunized mice. Data are shown as means ± SE. Statistical analyses used One-way-ANOVA followed by Bonferroni’s multiple comparison test. *** p

    Journal: Scientific Reports

    Article Title: Growth arrested live-attenuated Leishmania infantum KHARON1 null mutants display cytokinesis defect and protective immunity in mice

    doi: 10.1038/s41598-018-30076-7

    Figure Lengend Snippet: Humoral and cellular immune responses induced by different schemes of vaccination with Δ Likh1 . Humoral immune response was evaluated by conventional ELISA measurement of serum levels of different isotypes of Li WT SLA-specific antibodies (IgG total , IgG 1 and IgG 2a ). ( a ) Seroconversion of mice 20 days post-immunization with Δ Likh1 . ( b ) IgG2a:IgG1 ratio between IV and SC prime-boosted groups. ( c – h ) Levels of cytokines in pg/mL in the supernatant of Li WT SLA-stimulated splenocytes. The concentration of Li WT SLA in the experiments was 25 μg/mL and splenocytes were stimulated for 72 h. CpG ODN at 5 μg/mL was used as a positive control of a Th1-response and α-MEM medium with 20% of FBS was used as negative control. Cytokines were measured by flow cytometry with the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA, USA) following manufacturer instructions. The acquisition and data analyses were made in BD FACSVerse Instrument and FCAP Array Software v3. The detection limit of IL-2, IL-4, IL-6, IFN-γ, TNF, IL-17A and IL-10 cytokines were respectively 0.1; 0.03; 1.4; 0.5; 0.9; 0.8 and 16.8 pg/mL as well as cytokines secreted by Li WT SLA-stimulated splenocytes from immunized mice. Data are shown as means ± SE. Statistical analyses used One-way-ANOVA followed by Bonferroni’s multiple comparison test. *** p

    Article Snippet: Promastigote forms of L. infantum (MHOM/MA/67/ITMAP-263) were cultured in α-MEM medium (Gibco/Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum, 5 μg/mL of hemin and 5 μM of biopterin, in pH 7 at 26 °C.

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Concentration Assay, Positive Control, Negative Control, Flow Cytometry, Cytometry, Crocin Bleaching Assay, FCAP Assay, Software

    C3aR/C5aR are required for efficient OC differentiation . (A) 2 × 10 6 WT, C3aR −/− , C5aR −/− , and C3aR −/− C5aR −/− BM cells were cultured in α-MEM/10% heat-inactivated media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 . OCs were stained, and TRAP-positive cells in each well were counted under a microscope. (B) WT BM cells (2 × 10 6 ) were cultured in α-MEM/10% heat-inactivated FBS media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 in the presence of placebo (control), C3aRA, C5aRA, or C3aRA·C5aRA. OCs were stained, and TRAP-positive cells in each well were counted under a microscope. Results are representative from 2 independent experiments performed in triplicate. Data are mean ± SD * P

    Journal: Blood

    Article Title: Efficient osteoclast differentiation requires local complement activation

    doi: 10.1182/blood-2010-01-263590

    Figure Lengend Snippet: C3aR/C5aR are required for efficient OC differentiation . (A) 2 × 10 6 WT, C3aR −/− , C5aR −/− , and C3aR −/− C5aR −/− BM cells were cultured in α-MEM/10% heat-inactivated media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 . OCs were stained, and TRAP-positive cells in each well were counted under a microscope. (B) WT BM cells (2 × 10 6 ) were cultured in α-MEM/10% heat-inactivated FBS media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 in the presence of placebo (control), C3aRA, C5aRA, or C3aRA·C5aRA. OCs were stained, and TRAP-positive cells in each well were counted under a microscope. Results are representative from 2 independent experiments performed in triplicate. Data are mean ± SD * P

    Article Snippet: For OC differentiation, 2 × 106 BM cells were cultured in complete α-MEM medium in wells of a 24-well plate together with 1 × 10−8 M 1,25(OH)2 vitamin D3 (Cayman Chemical) as described before.

    Techniques: Cell Culture, Staining, Microscopy

    The alternative pathway of complement activation is required efficient OC differentiation . Aliquots of 2 × 10 6 WT and factor D −/− BM cells were cultured in α-MEM/10% heat-inactivated FBS media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 . OCs were stained, and TRAP-positive cells in each well were counted under a microscope. Panels show representative TRAP-positive cells from WT (A), factor D −/− (B) BM cells, and total mononuclear (mono)/multinucleated (multi) TRAP-positive cells in each well (C). Results are representative from 2 independent experiments performed in triplicates. Data are mean ± SD: * P

    Journal: Blood

    Article Title: Efficient osteoclast differentiation requires local complement activation

    doi: 10.1182/blood-2010-01-263590

    Figure Lengend Snippet: The alternative pathway of complement activation is required efficient OC differentiation . Aliquots of 2 × 10 6 WT and factor D −/− BM cells were cultured in α-MEM/10% heat-inactivated FBS media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 . OCs were stained, and TRAP-positive cells in each well were counted under a microscope. Panels show representative TRAP-positive cells from WT (A), factor D −/− (B) BM cells, and total mononuclear (mono)/multinucleated (multi) TRAP-positive cells in each well (C). Results are representative from 2 independent experiments performed in triplicates. Data are mean ± SD: * P

    Article Snippet: For OC differentiation, 2 × 106 BM cells were cultured in complete α-MEM medium in wells of a 24-well plate together with 1 × 10−8 M 1,25(OH)2 vitamin D3 (Cayman Chemical) as described before.

    Techniques: Activation Assay, Cell Culture, Staining, Microscopy

    C3 produced by BM cells is required for efficient OC differentiation . Aliquots of 2 × 10 6 WT and C3 −/− BM cells were cultured in α-MEM/10% heat-inactivated FBS media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 . OCs were stained, and TRAP-positive cells in each well were counted under a microscope. Panels show representative TRAP-positive cells from WT (A), C3 −/− (B), BM cells, and total mononuclear (mono)/multinucleated (multi) TRAP-positive cells in each well (C). Results are representative from 3 independent experiments performed in triplicate. Data are mean ± SD: * P

    Journal: Blood

    Article Title: Efficient osteoclast differentiation requires local complement activation

    doi: 10.1182/blood-2010-01-263590

    Figure Lengend Snippet: C3 produced by BM cells is required for efficient OC differentiation . Aliquots of 2 × 10 6 WT and C3 −/− BM cells were cultured in α-MEM/10% heat-inactivated FBS media in each well of a 24-well plate together with 1 × 10 −8 M 1,25(OH) 2 vitamin D 3 . OCs were stained, and TRAP-positive cells in each well were counted under a microscope. Panels show representative TRAP-positive cells from WT (A), C3 −/− (B), BM cells, and total mononuclear (mono)/multinucleated (multi) TRAP-positive cells in each well (C). Results are representative from 3 independent experiments performed in triplicate. Data are mean ± SD: * P

    Article Snippet: For OC differentiation, 2 × 106 BM cells were cultured in complete α-MEM medium in wells of a 24-well plate together with 1 × 10−8 M 1,25(OH)2 vitamin D3 (Cayman Chemical) as described before.

    Techniques: Produced, Cell Culture, Staining, Microscopy