α-galactosaminidase Search Results


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  • 92
    New England Biolabs α n acetyl galactosaminidase
    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N <t>-acetylgalactosaminidase</t> treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red
    α N Acetyl Galactosaminidase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher gene exp hexa hs00942655 m1
    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N <t>-acetylgalactosaminidase</t> treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red
    Gene Exp Hexa Hs00942655 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore chicken liver n acetyl α galactosaminidase
    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N <t>-acetylgalactosaminidase</t> treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red
    Chicken Liver N Acetyl α Galactosaminidase, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Proteintech anti hexosaminidase
    Ionomycin treatment increases in cathepsin-D and <t>β-hexosaminidase</t> exocytosis in epiphyseal chondrocytes. Conditioned medium and cell lysates from primary mouse epiphyseal chondrocytes treated with or without 10 µM ionomycin and 10 mM EGTA were analysed by Western blotting for cathepsin D (A) or β-hexosaminidase (B) Three independent experiments were done with cells from 3 litters of mice.
    Anti Hexosaminidase, supplied by Proteintech, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore α n acetyl galactosaminidase
    Ionomycin treatment increases in cathepsin-D and <t>β-hexosaminidase</t> exocytosis in epiphyseal chondrocytes. Conditioned medium and cell lysates from primary mouse epiphyseal chondrocytes treated with or without 10 µM ionomycin and 10 mM EGTA were analysed by Western blotting for cathepsin D (A) or β-hexosaminidase (B) Three independent experiments were done with cells from 3 litters of mice.
    α N Acetyl Galactosaminidase, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Prestwick Chemical n acetyl α glucosaminidase
    Ionomycin treatment increases in cathepsin-D and <t>β-hexosaminidase</t> exocytosis in epiphyseal chondrocytes. Conditioned medium and cell lysates from primary mouse epiphyseal chondrocytes treated with or without 10 µM ionomycin and 10 mM EGTA were analysed by Western blotting for cathepsin D (A) or β-hexosaminidase (B) Three independent experiments were done with cells from 3 litters of mice.
    N Acetyl α Glucosaminidase, supplied by Prestwick Chemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Novus Biologicals hexosaminidase a hexa antibody
    Ionomycin treatment increases in cathepsin-D and <t>β-hexosaminidase</t> exocytosis in epiphyseal chondrocytes. Conditioned medium and cell lysates from primary mouse epiphyseal chondrocytes treated with or without 10 µM ionomycin and 10 mM EGTA were analysed by Western blotting for cathepsin D (A) or β-hexosaminidase (B) Three independent experiments were done with cells from 3 litters of mice.
    Hexosaminidase A Hexa Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cusabio porcine alpha n acetyl glucosaminidase elisa kit
    Ionomycin treatment increases in cathepsin-D and <t>β-hexosaminidase</t> exocytosis in epiphyseal chondrocytes. Conditioned medium and cell lysates from primary mouse epiphyseal chondrocytes treated with or without 10 µM ionomycin and 10 mM EGTA were analysed by Western blotting for cathepsin D (A) or β-hexosaminidase (B) Three independent experiments were done with cells from 3 litters of mice.
    Porcine Alpha N Acetyl Glucosaminidase Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim n acetyl β glucosaminidase
    Ionomycin treatment increases in cathepsin-D and <t>β-hexosaminidase</t> exocytosis in epiphyseal chondrocytes. Conditioned medium and cell lysates from primary mouse epiphyseal chondrocytes treated with or without 10 µM ionomycin and 10 mM EGTA were analysed by Western blotting for cathepsin D (A) or β-hexosaminidase (B) Three independent experiments were done with cells from 3 litters of mice.
    N Acetyl β Glucosaminidase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    BioMarin n acetyl glucosaminidase naglu
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    N Acetyl Glucosaminidase Naglu, supplied by BioMarin, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Biotechnology Information hexosaminidase a deficiency genereview bethesda
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    Hexosaminidase A Deficiency Genereview Bethesda, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Boehringer Mannheim endo n acetyl glucosaminidase d
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    Endo N Acetyl Glucosaminidase D, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore 4 methylumbelliferyl n acetyl α glucosaminidase synthetic fluorogenic substrate
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    4 Methylumbelliferyl N Acetyl α Glucosaminidase Synthetic Fluorogenic Substrate, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore p nitrophenyl n acetyl α d glucosaminide
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    P Nitrophenyl N Acetyl α D Glucosaminide, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Novus Biologicals anti α n acetylglucosaminidase naglu antibody
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    Anti α N Acetylglucosaminidase Naglu Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    R&D Systems α naglu
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    α Naglu, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    R&D Systems α n acetylglucosaminidase
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    α N Acetylglucosaminidase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Santa Cruz Biotechnology α ncoat
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    α Ncoat, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Synageva n acetylglucosaminidase alpha rhnaglu
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    N Acetylglucosaminidase Alpha Rhnaglu, supplied by Synageva, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche peptide n glycosidase f α
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    Peptide N Glycosidase F α, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 4 methylumbelliferyl n acetyl α d glucosaminide
    <t>N-acetyl-glucosaminidase</t> <t>(NAGLU):</t> 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.
    4 Methylumbelliferyl N Acetyl α D Glucosaminide, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ProZyme exoglycosidase enzymes
    Overall analyses of N -glycans from purified Gpc1. The N -glycans present on Gpc1-WT ( A ), Gpc1-N79Q ( B ), and Gpc1-N116Q ( C ) as determined using <t>exoglycosidase</t> digestions and analysis on the 1.7-μm HILIC phase are shown. For each sample, the most
    Exoglycosidase Enzymes, supplied by ProZyme, used in various techniques. Bioz Stars score: 88/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red

    Journal: The Journal of Biological Chemistry

    Article Title: The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface *

    doi: 10.1074/jbc.M113.476531

    Figure Lengend Snippet: Binding of rCvGal1 to intact and glycosidase-treated hemocytes. Binding of rCvGal1 ( A ) or anti-blood group A antibody ( B ) to unattached hemocytes with α- N -acetylgalactosaminidase treatment ( GalNAc'ase , blue line ) or no treatment ( Untreated , red

    Article Snippet: PNGase F (from Flavobacterium meningosepticum ), O -glycosidase (cloned from Enterococcus faecalis and expressed in Escherichia coli ), α- N -acetylgalactosaminidase (cloned from Chryseobacterium meningosepticum and expressed in E. coli ), and β1,3-galactosidase (cloned from Xanthomonas manihotis and expressed in E. coli ) were obtained from New England Biolabs (Ipswich, MA).

    Techniques: Binding Assay

    Ionomycin treatment increases in cathepsin-D and β-hexosaminidase exocytosis in epiphyseal chondrocytes. Conditioned medium and cell lysates from primary mouse epiphyseal chondrocytes treated with or without 10 µM ionomycin and 10 mM EGTA were analysed by Western blotting for cathepsin D (A) or β-hexosaminidase (B) Three independent experiments were done with cells from 3 litters of mice.

    Journal: Biology Open

    Article Title: Evidence for lysosomal exocytosis and release of aggrecan-degrading hydrolases from hypertrophic chondrocytes, in vitro and in vivo

    doi: 10.1242/bio.2012547

    Figure Lengend Snippet: Ionomycin treatment increases in cathepsin-D and β-hexosaminidase exocytosis in epiphyseal chondrocytes. Conditioned medium and cell lysates from primary mouse epiphyseal chondrocytes treated with or without 10 µM ionomycin and 10 mM EGTA were analysed by Western blotting for cathepsin D (A) or β-hexosaminidase (B) Three independent experiments were done with cells from 3 litters of mice.

    Article Snippet: Monoclonal antibodies anti-LAMP1 (1D4B) from the Developmental Studies Hybridoma Bank, Iowa US, anti-chondroitin-4-sulphate (2B6) from Seikagaku Corporation, Tokyo, Japan and chondroitin-6-sulphate (3B3), a gift from Prof. Bruce Caterson, Cardiff Wales, were used in this study in addition to rabbit polyclonal antibodies anti-cathepsin D (EPR3057Y) and anti-VAMP7 (SYBL1) from Abcam Cambridge UK, anti-VAMP8 (Endobrevin) from Synaptic Systems Göttingen Germany, anti-hexosaminidase (alpha and beta chains) from Proteintech IL USA.

    Techniques: Western Blot, Mouse Assay

    N-acetyl-glucosaminidase (NAGLU): 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.

    Journal: Human mutation

    Article Title: Objective Assessment of the Evolutionary Action Equation for the Fitness Effect of Missense Mutations Across CAGI Blinded Contests

    doi: 10.1002/humu.23266

    Figure Lengend Snippet: N-acetyl-glucosaminidase (NAGLU): 165 missense mutants were transfected into HEK293 cells and the NAGLU activity was assessed using a fluorogenic substrate ( A ) The average EA scores for groups of 10 mutants with similar NAGLU activity. Two of the 165 mutants had no experimental assessment and they were omitted. The error bars note the standard error of the mean. ( B ) The average enzymatic activity of NAGLU for groups of 10 mutants with similar EA scores. The error bars note the standard error of the mean. ( C ) The performance of the submitted predictions, according to the overall rank of the CAGI assessor. The assessor used three tests for the overall assessment, the Root-Mean-Square Deviation, the Pearson's Correlation Coefficient, and the Spearman's Rank Correlation Coefficient. The CAGI assessor reported only the best performing method from each research group, since often multiple submissions from the same groups were based on the same original values using different scaling factors. This choice may favor groups with multiple submissions. ( D ) The Pearson's Correlation Coefficients for each submission, calculated by the authors. In addition to the submitted predictions which are shown as colored bars, we calculated Pearson's Correlation Coefficients for PolyPhen2 and SIFT, shown as dashed lines. ( E ) The area under the Receiver Operating Characteristic curve (AUC) for the submitted predictions as function of the threshold of NAGLU activity to separate into deleterious and neutral all 163 mutations or ( F ) the 77 mutations with an experimental standard deviation of σ=0.05 and less. The AUC values were calculated by the authors and they were colored according to the colors of the bars in (D). The dashed lines correspond to predictions from PolyPhen2 and SIFT.

    Article Snippet: The enzymatic activity of N-acetyl-glucosaminidase (NAGLU) for 165 missense mutations, which were exclusively found in the ExAC dataset , was assessed as the percentage of the wild-type NAGLU activity by BioMarin Pharmaceutical, Inc.

    Techniques: Transfection, Activity Assay, Standard Deviation

    Overall analyses of N -glycans from purified Gpc1. The N -glycans present on Gpc1-WT ( A ), Gpc1-N79Q ( B ), and Gpc1-N116Q ( C ) as determined using exoglycosidase digestions and analysis on the 1.7-μm HILIC phase are shown. For each sample, the most

    Journal: The Journal of Biological Chemistry

    Article Title: Structural Aspects of N-Glycosylations and the C-terminal Region in Human Glypican-1 *

    doi: 10.1074/jbc.M115.660878

    Figure Lengend Snippet: Overall analyses of N -glycans from purified Gpc1. The N -glycans present on Gpc1-WT ( A ), Gpc1-N79Q ( B ), and Gpc1-N116Q ( C ) as determined using exoglycosidase digestions and analysis on the 1.7-μm HILIC phase are shown. For each sample, the most

    Article Snippet: A panel of digestions for Gpc1 N -glycans that includes the most commonly used exoglycosidase enzymes (α-sialidase, α-fucosidase, β-galactosidase, β-hexosaminidase, and α-mannosidase) is shown in .

    Techniques: Purification, Hydrophilic Interaction Liquid Chromatography

    HILIC-FLD-UPLC profiles of Gpc1 N -glycans subjected to exoglycosidase digestions to assign their structures. UND represents the undigested pool of glycans; this pool was digested with sialidase ( ABS and NAN1 ), α-fucosidase ( BKF ), β-galactosidase

    Journal: The Journal of Biological Chemistry

    Article Title: Structural Aspects of N-Glycosylations and the C-terminal Region in Human Glypican-1 *

    doi: 10.1074/jbc.M115.660878

    Figure Lengend Snippet: HILIC-FLD-UPLC profiles of Gpc1 N -glycans subjected to exoglycosidase digestions to assign their structures. UND represents the undigested pool of glycans; this pool was digested with sialidase ( ABS and NAN1 ), α-fucosidase ( BKF ), β-galactosidase

    Article Snippet: A panel of digestions for Gpc1 N -glycans that includes the most commonly used exoglycosidase enzymes (α-sialidase, α-fucosidase, β-galactosidase, β-hexosaminidase, and α-mannosidase) is shown in .

    Techniques: Hydrophilic Interaction Liquid Chromatography