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    • cell cycle regulation sampler kit  (Cell Signaling Technology Inc)
    93
    Cell Signaling Technology Inc cell cycle regulation sampler kit
    Cell Cycle Regulation Sampler Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    streptococcus sp strain atcc 9932  (ATCC)
    93
    ATCC streptococcus sp strain atcc 9932
    Streptococcus Sp Strain Atcc 9932, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptococcus sp strain atcc 9932/product/ATCC
    Average 93 stars, based on 1 article reviews
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    streptococcus sp strain atcc 9932 - by Bioz Stars, 2023-03
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    9932 cdkn2a p16ink4a  (Abcam)
    86
    Abcam 9932 cdkn2a p16ink4a
    Primary antibodies used in this study.
    9932 Cdkn2a P16ink4a, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/9932 cdkn2a p16ink4a/product/Abcam
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    9932 cdkn1b p27kip1  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc 9932 cdkn1b p27kip1
    NUPR1 depletion causes premature senescence in vitro and represses tumorigenesis in vivo. (A) Representative images of GLB1 activity in NUPR1-depleted and control cells as indicated (left panels). Quantification of GLB1-positive cells was determined in 10 different fields from 3 independent experiments (mean ± SEM) (right panel). Scale bars: 10 µm. (B) NUPR1-depleted A549 cells were collected for cell cycle analysis by flow cytometry. The percentage of cells in G0/G1, S, and G2/M phases from 3 independent experiments is shown (right panel, mean ± SEM). (C) Western blot analysis of the indicated proteins in A549 and H460 cells infected with NUPR1 shRNA, with ACTB as a loading control. (D) Cellular proliferation of control and NUPR1-shRNA A549 cells was assessed using a 5-bromodeoxyuridine (BrdU) assay. The data are represented as the mean ± SEM of 6 experiments. (E) Clonogenic assays performed with control and NUPR1-shRNA A549 cells. A total of 1,500 cells were seeded in 24-well plates and grown for 2 wk. The graph shows the quantification of the mean number of colonies at different time point as indicated. ** P < 0.01 compared to control. (F) Western blot analysis of CASP3, cleaved CASP3, CASP7, CASP9, and ACTB in NUPR1-depleted A549 cells. (G) Western blot analysis of <t>CDKN1B</t> in A549 cells by NUPR1 depletion and/or its reexpression, with ACTB as a loading control. (H) A549 cells with lentivirus-delivered NUPR1 knockdown were subcutaneously implanted into female athymic nude mice (n = 6 for each experimental condition). The tumor image (left panel) on d 24 and tumor growth curve (right panel, mean ± SEM) are shown. ** P < 0.01 compared to control.
    9932 Cdkn1b P27kip1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/9932 cdkn1b p27kip1/product/Cell Signaling Technology Inc
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    xray software version 3 9932  (Biotique Systems)
    86
    Biotique Systems xray software version 3 9932
    NUPR1 depletion causes premature senescence in vitro and represses tumorigenesis in vivo. (A) Representative images of GLB1 activity in NUPR1-depleted and control cells as indicated (left panels). Quantification of GLB1-positive cells was determined in 10 different fields from 3 independent experiments (mean ± SEM) (right panel). Scale bars: 10 µm. (B) NUPR1-depleted A549 cells were collected for cell cycle analysis by flow cytometry. The percentage of cells in G0/G1, S, and G2/M phases from 3 independent experiments is shown (right panel, mean ± SEM). (C) Western blot analysis of the indicated proteins in A549 and H460 cells infected with NUPR1 shRNA, with ACTB as a loading control. (D) Cellular proliferation of control and NUPR1-shRNA A549 cells was assessed using a 5-bromodeoxyuridine (BrdU) assay. The data are represented as the mean ± SEM of 6 experiments. (E) Clonogenic assays performed with control and NUPR1-shRNA A549 cells. A total of 1,500 cells were seeded in 24-well plates and grown for 2 wk. The graph shows the quantification of the mean number of colonies at different time point as indicated. ** P < 0.01 compared to control. (F) Western blot analysis of CASP3, cleaved CASP3, CASP7, CASP9, and ACTB in NUPR1-depleted A549 cells. (G) Western blot analysis of <t>CDKN1B</t> in A549 cells by NUPR1 depletion and/or its reexpression, with ACTB as a loading control. (H) A549 cells with lentivirus-delivered NUPR1 knockdown were subcutaneously implanted into female athymic nude mice (n = 6 for each experimental condition). The tumor image (left panel) on d 24 and tumor growth curve (right panel, mean ± SEM) are shown. ** P < 0.01 compared to control.
    Xray Software Version 3 9932, supplied by Biotique Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xray software version 3 9932/product/Biotique Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xray software version 3 9932 - by Bioz Stars, 2023-03
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    Image Search Results


    Primary antibodies used in this study.

    Journal: Autophagy

    Article Title: NUPR1 maintains autolysosomal efflux by activating SNAP25 transcription in cancer cells

    doi: 10.1080/15548627.2017.1338556

    Figure Lengend Snippet: Primary antibodies used in this study.

    Article Snippet: DNA sequences for shRNA are listed in Table S2 . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Primary Antibody Dilution Ratio Supplier ATG5 (rabbit polyclonal) 1:1,000 Cell Signaling Technology, 2630 ACTB (mouse monoclonal) 1:3,000 Sigma-Aldrich, A-3853 BECN1(rabbit polyclonal) 1:2,000 Cell Signaling Technology, 3738 CASP3 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 9665 CDKN1A/p21Waf1 (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 9932 CDKN1B/p27Kip1(rabbit monoclonal) 1:2,000 Cell Signaling Technology, 9932 CDKN2A/p16INK4a (rabbit monoclonal) 1:2,000 Abcam, ab108349 CTSB (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-365558 CTSD (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-377299 Cleaved CASP3 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 9664 Cleaved CASP7 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 8438 Cleaved CASP9 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 7237 FLAG (mouse monoclonal) 1:4,000 Sigma-Aldrich, F3165 GAPDH (rabbit polyclonal) 1:3,000 Trevigen, 2275 HA (rabbit monoclonal) 1:2.000 Cell Signaling Technology, 3724S LC3B (rabbit polyclonal) 1:2,000 Sigma-Aldrich, L7543 MTOR (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 2983 NUPR1 (mouse monoclonal) 1:1,000 Abcam, ab87454 p-MTOR(S2448) (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 5536 PCNA (rabbit polyclonal) 1:1,000 Abcam, ab18197 SNAP25 (rabbit polyclonal) 1:1,000 Abcam, ab109105 SQSTM1/p62 (rabbit polyclonal) 1:1,000 Cell Signaling Technology, 7695 VAMP8 (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-166820 Open in a separate window Primary antibodies used in this study.

    Techniques:

    NUPR1 depletion causes premature senescence in vitro and represses tumorigenesis in vivo. (A) Representative images of GLB1 activity in NUPR1-depleted and control cells as indicated (left panels). Quantification of GLB1-positive cells was determined in 10 different fields from 3 independent experiments (mean ± SEM) (right panel). Scale bars: 10 µm. (B) NUPR1-depleted A549 cells were collected for cell cycle analysis by flow cytometry. The percentage of cells in G0/G1, S, and G2/M phases from 3 independent experiments is shown (right panel, mean ± SEM). (C) Western blot analysis of the indicated proteins in A549 and H460 cells infected with NUPR1 shRNA, with ACTB as a loading control. (D) Cellular proliferation of control and NUPR1-shRNA A549 cells was assessed using a 5-bromodeoxyuridine (BrdU) assay. The data are represented as the mean ± SEM of 6 experiments. (E) Clonogenic assays performed with control and NUPR1-shRNA A549 cells. A total of 1,500 cells were seeded in 24-well plates and grown for 2 wk. The graph shows the quantification of the mean number of colonies at different time point as indicated. ** P < 0.01 compared to control. (F) Western blot analysis of CASP3, cleaved CASP3, CASP7, CASP9, and ACTB in NUPR1-depleted A549 cells. (G) Western blot analysis of CDKN1B in A549 cells by NUPR1 depletion and/or its reexpression, with ACTB as a loading control. (H) A549 cells with lentivirus-delivered NUPR1 knockdown were subcutaneously implanted into female athymic nude mice (n = 6 for each experimental condition). The tumor image (left panel) on d 24 and tumor growth curve (right panel, mean ± SEM) are shown. ** P < 0.01 compared to control.

    Journal: Autophagy

    Article Title: NUPR1 maintains autolysosomal efflux by activating SNAP25 transcription in cancer cells

    doi: 10.1080/15548627.2017.1338556

    Figure Lengend Snippet: NUPR1 depletion causes premature senescence in vitro and represses tumorigenesis in vivo. (A) Representative images of GLB1 activity in NUPR1-depleted and control cells as indicated (left panels). Quantification of GLB1-positive cells was determined in 10 different fields from 3 independent experiments (mean ± SEM) (right panel). Scale bars: 10 µm. (B) NUPR1-depleted A549 cells were collected for cell cycle analysis by flow cytometry. The percentage of cells in G0/G1, S, and G2/M phases from 3 independent experiments is shown (right panel, mean ± SEM). (C) Western blot analysis of the indicated proteins in A549 and H460 cells infected with NUPR1 shRNA, with ACTB as a loading control. (D) Cellular proliferation of control and NUPR1-shRNA A549 cells was assessed using a 5-bromodeoxyuridine (BrdU) assay. The data are represented as the mean ± SEM of 6 experiments. (E) Clonogenic assays performed with control and NUPR1-shRNA A549 cells. A total of 1,500 cells were seeded in 24-well plates and grown for 2 wk. The graph shows the quantification of the mean number of colonies at different time point as indicated. ** P < 0.01 compared to control. (F) Western blot analysis of CASP3, cleaved CASP3, CASP7, CASP9, and ACTB in NUPR1-depleted A549 cells. (G) Western blot analysis of CDKN1B in A549 cells by NUPR1 depletion and/or its reexpression, with ACTB as a loading control. (H) A549 cells with lentivirus-delivered NUPR1 knockdown were subcutaneously implanted into female athymic nude mice (n = 6 for each experimental condition). The tumor image (left panel) on d 24 and tumor growth curve (right panel, mean ± SEM) are shown. ** P < 0.01 compared to control.

    Article Snippet: DNA sequences for shRNA are listed in Table S2 . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Primary Antibody Dilution Ratio Supplier ATG5 (rabbit polyclonal) 1:1,000 Cell Signaling Technology, 2630 ACTB (mouse monoclonal) 1:3,000 Sigma-Aldrich, A-3853 BECN1(rabbit polyclonal) 1:2,000 Cell Signaling Technology, 3738 CASP3 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 9665 CDKN1A/p21Waf1 (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 9932 CDKN1B/p27Kip1(rabbit monoclonal) 1:2,000 Cell Signaling Technology, 9932 CDKN2A/p16INK4a (rabbit monoclonal) 1:2,000 Abcam, ab108349 CTSB (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-365558 CTSD (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-377299 Cleaved CASP3 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 9664 Cleaved CASP7 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 8438 Cleaved CASP9 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 7237 FLAG (mouse monoclonal) 1:4,000 Sigma-Aldrich, F3165 GAPDH (rabbit polyclonal) 1:3,000 Trevigen, 2275 HA (rabbit monoclonal) 1:2.000 Cell Signaling Technology, 3724S LC3B (rabbit polyclonal) 1:2,000 Sigma-Aldrich, L7543 MTOR (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 2983 NUPR1 (mouse monoclonal) 1:1,000 Abcam, ab87454 p-MTOR(S2448) (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 5536 PCNA (rabbit polyclonal) 1:1,000 Abcam, ab18197 SNAP25 (rabbit polyclonal) 1:1,000 Abcam, ab109105 SQSTM1/p62 (rabbit polyclonal) 1:1,000 Cell Signaling Technology, 7695 VAMP8 (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-166820 Open in a separate window Primary antibodies used in this study.

    Techniques: In Vitro, In Vivo, Activity Assay, Cell Cycle Assay, Flow Cytometry, Western Blot, Infection, shRNA, BrdU Staining

    Primary antibodies used in this study.

    Journal: Autophagy

    Article Title: NUPR1 maintains autolysosomal efflux by activating SNAP25 transcription in cancer cells

    doi: 10.1080/15548627.2017.1338556

    Figure Lengend Snippet: Primary antibodies used in this study.

    Article Snippet: DNA sequences for shRNA are listed in Table S2 . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Primary Antibody Dilution Ratio Supplier ATG5 (rabbit polyclonal) 1:1,000 Cell Signaling Technology, 2630 ACTB (mouse monoclonal) 1:3,000 Sigma-Aldrich, A-3853 BECN1(rabbit polyclonal) 1:2,000 Cell Signaling Technology, 3738 CASP3 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 9665 CDKN1A/p21Waf1 (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 9932 CDKN1B/p27Kip1(rabbit monoclonal) 1:2,000 Cell Signaling Technology, 9932 CDKN2A/p16INK4a (rabbit monoclonal) 1:2,000 Abcam, ab108349 CTSB (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-365558 CTSD (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-377299 Cleaved CASP3 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 9664 Cleaved CASP7 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 8438 Cleaved CASP9 (rabbit polyclonal) 1:1000 Cell Signaling Technology, 7237 FLAG (mouse monoclonal) 1:4,000 Sigma-Aldrich, F3165 GAPDH (rabbit polyclonal) 1:3,000 Trevigen, 2275 HA (rabbit monoclonal) 1:2.000 Cell Signaling Technology, 3724S LC3B (rabbit polyclonal) 1:2,000 Sigma-Aldrich, L7543 MTOR (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 2983 NUPR1 (mouse monoclonal) 1:1,000 Abcam, ab87454 p-MTOR(S2448) (rabbit monoclonal) 1:2,000 Cell Signaling Technology, 5536 PCNA (rabbit polyclonal) 1:1,000 Abcam, ab18197 SNAP25 (rabbit polyclonal) 1:1,000 Abcam, ab109105 SQSTM1/p62 (rabbit polyclonal) 1:1,000 Cell Signaling Technology, 7695 VAMP8 (mouse monoclonal) 1:1,000 Santa Cruz Biotechnology, sc-166820 Open in a separate window Primary antibodies used in this study.

    Techniques: