Journal: Applied and Environmental Microbiology
Article Title: Metabolic Engineering of Saccharomyces cerevisiae for Astaxanthin Production and Oxidative Stress Tolerance
Figure Lengend Snippet: Biosynthetic pathway for astaxanthin engineered in S. cerevisiae. This pathway shows systematic astaxanthin biosynthesis from the mevalonate pathway in S. cerevisiae, into which the carotenogenic genes from X. dendrorhous shown in parentheses and bacteria were introduced. The endogenous genes of S. cerevisiae are underlined. The black, white, and gray thick arrows indicate the reactions catalyzed by the enzymes encoded by crtW, crtZ, and crtS, respectively. Although the crtS-mediated biosynthesis of astaxanthin has been poorly understood, the pathway proposed by Andrewes et al. (4) is shown in this figure. DMAPP, dimethylallyl pyrophosphate; FPP, farnesyl pyrophosphate; IPP, isopentenyl pyrophosphate.
Article Snippet: pAD-crtS , cDNA corresponding to crtS from X. dendrorhous ATCC 24202 in pAD4 , This study.