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    • 14 3 3ζ  (Cell Signaling Technology Inc)
    93
    Cell Signaling Technology Inc 14 3 3ζ
    14 3 3ζ, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/14 3 3ζ/product/Cell Signaling Technology Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    14 3 3ζ - by Bioz Stars, 2023-04
    93/100 stars
    		  Buy from Supplier
    n 7413 trilink  (TriLink)
    86
    TriLink n 7413 trilink
    Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 <t>(CC1)</t> capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.
    N 7413 Trilink, supplied by TriLink, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n 7413 trilink/product/TriLink
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n 7413 trilink - by Bioz Stars, 2023-04
    86/100 stars
    		  Buy from Supplier
    7403 7413 kermel chemical  (Kermel Inc)
    86
    Kermel Inc 7403 7413 kermel chemical
    Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 <t>(CC1)</t> capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.
    7403 7413 Kermel Chemical, supplied by Kermel Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7403 7413 kermel chemical/product/Kermel Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7403 7413 kermel chemical - by Bioz Stars, 2023-04
    86/100 stars
    		  Buy from Supplier
    7410 7413 scheme 1 existing approaches  (Georg Thieme Verlag KG)
    86
    Georg Thieme Verlag KG 7410 7413 scheme 1 existing approaches
    Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 <t>(CC1)</t> capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.
    7410 7413 Scheme 1 Existing Approaches, supplied by Georg Thieme Verlag KG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7410 7413 scheme 1 existing approaches/product/Georg Thieme Verlag KG
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7410 7413 scheme 1 existing approaches - by Bioz Stars, 2023-04
    86/100 stars
    		  Buy from Supplier
    n 7413 ponceau s sigma p3504 schneider  (Millipore)
    86
    Millipore n 7413 ponceau s sigma p3504 schneider
    Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 <t>(CC1)</t> capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.
    N 7413 Ponceau S Sigma P3504 Schneider, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n 7413 ponceau s sigma p3504 schneider/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n 7413 ponceau s sigma p3504 schneider - by Bioz Stars, 2023-04
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 (CC1) capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.

    Journal: Pharmaceutics

    Article Title: Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA

    doi: 10.3390/pharmaceutics14020328

    Figure Lengend Snippet: Ribozyme-mediated cleavage effectively assesses capping efficiencies of IVT mRNAs of different lengths. The image shows 21% PAGE, 8 M urea visualization of Rz5-cleaved and silica-column purified TEV, m1Ψ-containing, beta-S-ARCA (D1) or CleanCap ® Reagent AG (3′ OMe), cap1 (CC1) capped IVT mRNAs. The IVT mRNAs ranged from 1.1 to 9.4 kb in length. Rz: Ribozyme; 5′CP: 5′ cleavage product.

    Article Snippet: UPLC and MS profiles of CleanCap ® Reagent AG (3′ OMe), N-7413 TriLink (CC1) EPO mRNA ( ) showed >99% capping, which is also in agreement with >90% capping efficiency obtained by screening of >100 CC1-capped IVT mRNAs using 21% PAGE, 8 M urea ( and data not shown).

    Techniques: Purification

    LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified CleanCap ® Reagent AG (3′ OMe) = cap1 (CC1), human alpha globin (hAg), m1Ψ-containing erythropoietin (EPO) mRNA. Expected capped product (MW = 8347) is detected in >99%. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; AU: Arbitrary units.

    Journal: Pharmaceutics

    Article Title: Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA

    doi: 10.3390/pharmaceutics14020328

    Figure Lengend Snippet: LC–MS analysis of ribozyme-mediated cleavage products for quantification and characterization of capped products from Rz1 cleaved and silica-column purified CleanCap ® Reagent AG (3′ OMe) = cap1 (CC1), human alpha globin (hAg), m1Ψ-containing erythropoietin (EPO) mRNA. Expected capped product (MW = 8347) is detected in >99%. ( A ) UPLC and ( B ) MS profiles. Rz: Ribozyme; Cap: Capped product; AU: Arbitrary units.

    Article Snippet: UPLC and MS profiles of CleanCap ® Reagent AG (3′ OMe), N-7413 TriLink (CC1) EPO mRNA ( ) showed >99% capping, which is also in agreement with >90% capping efficiency obtained by screening of >100 CC1-capped IVT mRNAs using 21% PAGE, 8 M urea ( and data not shown).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Purification