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Image Search Results
Journal: Journal of Histochemistry and Cytochemistry
Article Title: Immunohistochemical Validation and Expression Profiling of NKG2D Ligands in a Wide Spectrum of Human Epithelial Neoplasms
doi: 10.1369/0022155414563800
Figure Lengend Snippet: Primer Sequences for Cloning of FLAG-tagged UL16-binding proteins (ULBPs).
Article Snippet: Antibodies against NKG2D ligands were obtained from the following sources: anti-MICA/B monoclonal antibody (mAb) from Biolegend (San Diego, CA); anti-ULBP1 and anti-ULBP3 goat polyclonal antibodies (pAbs) from R&D Systems (Minneapolis, MN);
Techniques: Clone Assay
Journal: Journal of Histochemistry and Cytochemistry
Article Title: Immunohistochemical Validation and Expression Profiling of NKG2D Ligands in a Wide Spectrum of Human Epithelial Neoplasms
doi: 10.1369/0022155414563800
Figure Lengend Snippet: Comparison of NKG2D Ligand Expression between Non-neoplastic and Neoplastic Tissues.
Article Snippet: Antibodies against NKG2D ligands were obtained from the following sources: anti-MICA/B monoclonal antibody (mAb) from Biolegend (San Diego, CA); anti-ULBP1 and anti-ULBP3 goat polyclonal antibodies (pAbs) from R&D Systems (Minneapolis, MN);
Techniques: Expressing
Journal: Journal of Histochemistry and Cytochemistry
Article Title: Immunohistochemical Validation and Expression Profiling of NKG2D Ligands in a Wide Spectrum of Human Epithelial Neoplasms
doi: 10.1369/0022155414563800
Figure Lengend Snippet: Validation of antibodies using ULBP-transfected COS7 cells. We confirmed the specificity of all antibodies against UL16-binding proteins (ULBP) using western blotting with cell lysates (A) and immunohistochemistry with a FFPE cell block (B) prepared from each ULBP transfectant, respectively. The anti-ULBP2 antibody cross-reacted with ULBP5 and ULBP6 in western blotting but only with ULBP6 in FFPE. Therefore, this antibody was used to mark ULBP2 and ULBP6. Scale, 50 μm.
Article Snippet: Antibodies against NKG2D ligands were obtained from the following sources: anti-MICA/B monoclonal antibody (mAb) from Biolegend (San Diego, CA); anti-ULBP1 and anti-ULBP3 goat polyclonal antibodies (pAbs) from R&D Systems (Minneapolis, MN);
Techniques: Transfection, Binding Assay, Western Blot, Immunohistochemistry, Blocking Assay
Journal: Journal of Histochemistry and Cytochemistry
Article Title: Immunohistochemical Validation and Expression Profiling of NKG2D Ligands in a Wide Spectrum of Human Epithelial Neoplasms
doi: 10.1369/0022155414563800
Figure Lengend Snippet: Diverse expression of NKG2D ligands in non-neoplastic prostate tissue. The upper panels show immunohistochemistry for ULBP1 (A), ULBP2/6 (B), and ULBP4 (C) as positive, and the lower panels for MICA/B (D), ULBP3 (E), and ULBP5 (F) as negative. Scale, 100 μm.
Article Snippet: Antibodies against NKG2D ligands were obtained from the following sources: anti-MICA/B monoclonal antibody (mAb) from Biolegend (San Diego, CA); anti-ULBP1 and anti-ULBP3 goat polyclonal antibodies (pAbs) from R&D Systems (Minneapolis, MN);
Techniques: Expressing, Immunohistochemistry