Journal: Apoptosis

Article Title: Terfenadine induces apoptosis and autophagy in melanoma cells through ROS-dependent and -independent mechanisms

doi: 10.1007/s10495-011-0640-y

Figure Lengend Snippet: Effect of Atg 7 downregulation in TEF-induced cytotoxicity. a Assessment of transfection efficiency using SignalSilence Control siRNA (Fluorescein Conjugate), that also serves as a negative control. Cells were viewed under a confocal microscope. b Down-regulation of Atg 7 protein following transfection with specific Atg 7 siRNA. 50 μg of total cell extracts of transfected and non transfected cells were loaded per lane, separated by electrophoresis and analyzed by immunoblotting. c Atg 7 down-regulation partially prevented TEF-induced cytotoxicity. Cell viability was determined after 48 h of siRNA transfection. * P values < 0.05

Article Snippet: A375 and HT144 melanoma cells were transfected by means of the SignalSilence Atg7 siRNA I (Cell Signalling Technology, Inc., Danvers, MA, USA) using Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) as transfection agent, according to the manufacturer’s instructions.

Techniques: Transfection, Negative Control, Microscopy, Electrophoresis, Western Blot