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  • 91
    Bio-Techne corporation assays 10 × genomics chromium single cell library kit v2 10 × genomics
    Assays 10 × Genomics Chromium Single Cell Library Kit V2 10 × Genomics, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/assays 10 × genomics chromium single cell library kit v2 10 × genomics/product/Bio-Techne corporation
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    assays 10 × genomics chromium single cell library kit v2 10 × genomics - by Bioz Stars, 2024-06
    91/100 stars
      Buy from Supplier

    91
    Cell Signaling Technology Inc β catenin sirna ii
    PPI suppressed osteosarcoma cells by specifically <t>inactivating</t> <t>Wnt/β-catenin</t> signaling pathway. ( A ) RT-PCR analysis of β-catenin expression level in matched human osteosarcoma tissues (tumors) and adjacent noncancerous tissues (normal) from 3 patients. ( B ) 143-B cells and ( C ) HOS cells were respectively treated with 0.8 μM PPI for indicated times, and expressions of test proteins were examined by western blotting analysis, β-actin was used as loading control, and the full-length blots were included in the supplementary information file as Figures and . 143-B cells were pretreated with 4 μM CHIR9902 (the specific GSK-3β inhibitor) for 24 h before exposed to 0.8 μM PPI for another 48 h, the combined CHIR and PPI treatments result in rescued ( D ) active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ) and cell viability ( E ) compared to the PPI treatment alone in 143-B osteosarcoma cells. 143-B cells were transfected with either <t>small</t> <t>interfering</t> <t>RNA-targeting</t> β-catenin (si-β-catenin) or si-control for 48 h before exposed to 0.8 μM PPI for another 24 h, si-β-catenin potentiated PPI induced ( F ) down-regulation of active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ), ( G ) decrease of cell viability and ( H ) inhibition of migration of 143-B osteosarcoma cells induced by PPI. * p < 0.05, ** p < 0.01, *** p < 0.001, versus the control.
    β Catenin Sirna Ii, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β catenin sirna ii/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    β catenin sirna ii - by Bioz Stars, 2024-06
    91/100 stars
      Buy from Supplier

    86
    Integran Technologies Inc co pending application integran 6238
    PPI suppressed osteosarcoma cells by specifically <t>inactivating</t> <t>Wnt/β-catenin</t> signaling pathway. ( A ) RT-PCR analysis of β-catenin expression level in matched human osteosarcoma tissues (tumors) and adjacent noncancerous tissues (normal) from 3 patients. ( B ) 143-B cells and ( C ) HOS cells were respectively treated with 0.8 μM PPI for indicated times, and expressions of test proteins were examined by western blotting analysis, β-actin was used as loading control, and the full-length blots were included in the supplementary information file as Figures and . 143-B cells were pretreated with 4 μM CHIR9902 (the specific GSK-3β inhibitor) for 24 h before exposed to 0.8 μM PPI for another 48 h, the combined CHIR and PPI treatments result in rescued ( D ) active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ) and cell viability ( E ) compared to the PPI treatment alone in 143-B osteosarcoma cells. 143-B cells were transfected with either <t>small</t> <t>interfering</t> <t>RNA-targeting</t> β-catenin (si-β-catenin) or si-control for 48 h before exposed to 0.8 μM PPI for another 24 h, si-β-catenin potentiated PPI induced ( F ) down-regulation of active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ), ( G ) decrease of cell viability and ( H ) inhibition of migration of 143-B osteosarcoma cells induced by PPI. * p < 0.05, ** p < 0.01, *** p < 0.001, versus the control.
    Co Pending Application Integran 6238, supplied by Integran Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/co pending application integran 6238/product/Integran Technologies Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    co pending application integran 6238 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher 6238 issn 1997 5902 conclusion
    PPI suppressed osteosarcoma cells by specifically <t>inactivating</t> <t>Wnt/β-catenin</t> signaling pathway. ( A ) RT-PCR analysis of β-catenin expression level in matched human osteosarcoma tissues (tumors) and adjacent noncancerous tissues (normal) from 3 patients. ( B ) 143-B cells and ( C ) HOS cells were respectively treated with 0.8 μM PPI for indicated times, and expressions of test proteins were examined by western blotting analysis, β-actin was used as loading control, and the full-length blots were included in the supplementary information file as Figures and . 143-B cells were pretreated with 4 μM CHIR9902 (the specific GSK-3β inhibitor) for 24 h before exposed to 0.8 μM PPI for another 48 h, the combined CHIR and PPI treatments result in rescued ( D ) active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ) and cell viability ( E ) compared to the PPI treatment alone in 143-B osteosarcoma cells. 143-B cells were transfected with either <t>small</t> <t>interfering</t> <t>RNA-targeting</t> β-catenin (si-β-catenin) or si-control for 48 h before exposed to 0.8 μM PPI for another 24 h, si-β-catenin potentiated PPI induced ( F ) down-regulation of active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ), ( G ) decrease of cell viability and ( H ) inhibition of migration of 143-B osteosarcoma cells induced by PPI. * p < 0.05, ** p < 0.01, *** p < 0.001, versus the control.
    6238 Issn 1997 5902 Conclusion, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6238 issn 1997 5902 conclusion/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    6238 issn 1997 5902 conclusion - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology rabbit anti cks1 sc 6238
    FGFR1 signaling influences the expression of S-phase kinase-associated protein two (Skp2) but not the release of <t>Cks1.</t> (A): Cells were transfected with vectors coexpressing a constitutively active form of FGFR1 (Fc-FGFR1), wild type (wt) FRS2, and wt Cks1. Blot depicts immunoprecipitation of Fc-R1TK (constitutively active FGFR1) 24 hours after cell synchronization and release into maintenance media alone (CTRL), or supplemented with DMSO vehicle (D) or SU5402 (SU). (B): Western blot analysis of Skp2 expression after cell synchronization and release into DMSO vehicle or SU5402 supplemented media. (C): Hypothesized pathways for FGFR1 control of cell cycle progression in hMSCs. Abbreviations: AKT, protein kinase B; Cks1, cyclin-dependent kinase subunit one; CDK, cyclin dependent kinase; FRS, fibroblast receptor substrate; FGFR1, fibroblast growth factor; ERK, extracellular signal-regulated kinase; PI3K, phosphatidylinositide 3-kinases; Rb, retinoblastoma.
    Rabbit Anti Cks1 Sc 6238, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cks1 sc 6238/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cks1 sc 6238 - by Bioz Stars, 2024-06
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    Image Search Results


    PPI suppressed osteosarcoma cells by specifically inactivating Wnt/β-catenin signaling pathway. ( A ) RT-PCR analysis of β-catenin expression level in matched human osteosarcoma tissues (tumors) and adjacent noncancerous tissues (normal) from 3 patients. ( B ) 143-B cells and ( C ) HOS cells were respectively treated with 0.8 μM PPI for indicated times, and expressions of test proteins were examined by western blotting analysis, β-actin was used as loading control, and the full-length blots were included in the supplementary information file as Figures and . 143-B cells were pretreated with 4 μM CHIR9902 (the specific GSK-3β inhibitor) for 24 h before exposed to 0.8 μM PPI for another 48 h, the combined CHIR and PPI treatments result in rescued ( D ) active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ) and cell viability ( E ) compared to the PPI treatment alone in 143-B osteosarcoma cells. 143-B cells were transfected with either small interfering RNA-targeting β-catenin (si-β-catenin) or si-control for 48 h before exposed to 0.8 μM PPI for another 24 h, si-β-catenin potentiated PPI induced ( F ) down-regulation of active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ), ( G ) decrease of cell viability and ( H ) inhibition of migration of 143-B osteosarcoma cells induced by PPI. * p < 0.05, ** p < 0.01, *** p < 0.001, versus the control.

    Journal: Scientific Reports

    Article Title: Polyphyllin I suppresses human osteosarcoma growth by inactivation of Wnt/β-catenin pathway in vitro and in vivo

    doi: 10.1038/s41598-017-07194-9

    Figure Lengend Snippet: PPI suppressed osteosarcoma cells by specifically inactivating Wnt/β-catenin signaling pathway. ( A ) RT-PCR analysis of β-catenin expression level in matched human osteosarcoma tissues (tumors) and adjacent noncancerous tissues (normal) from 3 patients. ( B ) 143-B cells and ( C ) HOS cells were respectively treated with 0.8 μM PPI for indicated times, and expressions of test proteins were examined by western blotting analysis, β-actin was used as loading control, and the full-length blots were included in the supplementary information file as Figures and . 143-B cells were pretreated with 4 μM CHIR9902 (the specific GSK-3β inhibitor) for 24 h before exposed to 0.8 μM PPI for another 48 h, the combined CHIR and PPI treatments result in rescued ( D ) active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ) and cell viability ( E ) compared to the PPI treatment alone in 143-B osteosarcoma cells. 143-B cells were transfected with either small interfering RNA-targeting β-catenin (si-β-catenin) or si-control for 48 h before exposed to 0.8 μM PPI for another 24 h, si-β-catenin potentiated PPI induced ( F ) down-regulation of active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ), ( G ) decrease of cell viability and ( H ) inhibition of migration of 143-B osteosarcoma cells induced by PPI. * p < 0.05, ** p < 0.01, *** p < 0.001, versus the control.

    Article Snippet: Briefly, 143-B cells were co-transfected with either small interfering RNA-targeting β-catenin (100 nM si-β-catenin) or 100 nM si-control for 72 h, using Lipofectamine 2000 (Invitrogen). β-catenin siRNA II (#6238) was bought from Cell Signaling Technology (Danvers, USA). si-control was designed and produced by Shanghai GenePharma Co.,Ltd (Shanghai, China).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Transfection, Small Interfering RNA, Inhibition, Migration

    FGFR1 signaling influences the expression of S-phase kinase-associated protein two (Skp2) but not the release of Cks1. (A): Cells were transfected with vectors coexpressing a constitutively active form of FGFR1 (Fc-FGFR1), wild type (wt) FRS2, and wt Cks1. Blot depicts immunoprecipitation of Fc-R1TK (constitutively active FGFR1) 24 hours after cell synchronization and release into maintenance media alone (CTRL), or supplemented with DMSO vehicle (D) or SU5402 (SU). (B): Western blot analysis of Skp2 expression after cell synchronization and release into DMSO vehicle or SU5402 supplemented media. (C): Hypothesized pathways for FGFR1 control of cell cycle progression in hMSCs. Abbreviations: AKT, protein kinase B; Cks1, cyclin-dependent kinase subunit one; CDK, cyclin dependent kinase; FRS, fibroblast receptor substrate; FGFR1, fibroblast growth factor; ERK, extracellular signal-regulated kinase; PI3K, phosphatidylinositide 3-kinases; Rb, retinoblastoma.

    Journal: Stem cells (Dayton, Ohio)

    Article Title: FGFR1 Signaling Stimulates Proliferation of Human Mesenchymal Stem Cells by Inhibiting the Cyclin-Dependent Kinase Inhibitors p21 Waf1 and p27 Kip1

    doi: 10.1002/stem.1514

    Figure Lengend Snippet: FGFR1 signaling influences the expression of S-phase kinase-associated protein two (Skp2) but not the release of Cks1. (A): Cells were transfected with vectors coexpressing a constitutively active form of FGFR1 (Fc-FGFR1), wild type (wt) FRS2, and wt Cks1. Blot depicts immunoprecipitation of Fc-R1TK (constitutively active FGFR1) 24 hours after cell synchronization and release into maintenance media alone (CTRL), or supplemented with DMSO vehicle (D) or SU5402 (SU). (B): Western blot analysis of Skp2 expression after cell synchronization and release into DMSO vehicle or SU5402 supplemented media. (C): Hypothesized pathways for FGFR1 control of cell cycle progression in hMSCs. Abbreviations: AKT, protein kinase B; Cks1, cyclin-dependent kinase subunit one; CDK, cyclin dependent kinase; FRS, fibroblast receptor substrate; FGFR1, fibroblast growth factor; ERK, extracellular signal-regulated kinase; PI3K, phosphatidylinositide 3-kinases; Rb, retinoblastoma.

    Article Snippet: Rabbit anti-Cks1 (sc-6238) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, http://www.scbt.com ).

    Techniques: Expressing, Transfection, Immunoprecipitation, Western Blot