[56970 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • goat α mouse igg h l hrp  (Cell Signaling Technology Inc)
    98
    Cell Signaling Technology Inc goat α mouse igg h l hrp
    Goat α Mouse Igg H L Hrp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat α mouse igg h l hrp/product/Cell Signaling Technology Inc
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat α mouse igg h l hrp - by Bioz Stars, 2023-03
    98/100 stars
    		  Buy from Supplier
    anti oma1  (Novus Biologicals)
    90
    Novus Biologicals anti oma1
    C11orf83, like OPA1, is cleaved by <t>OMA1</t> under ΔΨ loss. (A) Analysis of the stress-induced proteolysis of C11orf83 compared to OPA1. HeLa cells expressing C11orf83-V5 were treated with mitochondrion-damaging agents for 3 h, and cell lysates were analyzed by immunoblotting after SDS-PAGE. β-Catenin and SDHB were used as loading controls for cytosolic and mitochondrial proteins. Stress-induced cleavage of OPA1 and C11orf83 was observed by antibodies against OPA1 and the V5 tag. ROS, reactive oxygen species. (B) Analysis of the sensitivity of CCCP-induced cleavage of C11orf83 to various protease inhibitors. HeLa cells expressing C11orf83-V5 were incubated with CCCP with or without protease inhibitors. Cells lysates were fractionated by SDS-PAGE and analyzed by immunoblotting. β-Catenin and SDHB were used as loading controls. The prevention of C11orf83-V5 proteolysis was monitored thanks to an antibody against the V5 tag. (C) OMA1 involvement in the stress-induced cleavage of C11orf83. HeLa cells were transfected with C11orf83-V5 and treated with control siRNA or siRNA against OMA1. Forty-eight hours after siRNA transfection, cells were incubated with or without CCCP and cell lysates were analyzed by immunoblotting, after SDS-PAGE, using antibodies against OMA1, OPA1, and the V5 tag. GAPDH was used as a loading control. The two bands detected by the antibody against OPA1 in the absence of CCCP correspond to short and long OPA1 isoforms. The degradation of endogenous C11orf83 under CCCP treatment (lane 3) was prevented by the depletion of OMA1 (lane 4).
    Anti Oma1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti oma1/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti oma1 - by Bioz Stars, 2023-03
    90/100 stars
    		  Buy from Supplier
    igg control sepharose bead conjugate  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc igg control sepharose bead conjugate
    C11orf83, like OPA1, is cleaved by <t>OMA1</t> under ΔΨ loss. (A) Analysis of the stress-induced proteolysis of C11orf83 compared to OPA1. HeLa cells expressing C11orf83-V5 were treated with mitochondrion-damaging agents for 3 h, and cell lysates were analyzed by immunoblotting after SDS-PAGE. β-Catenin and SDHB were used as loading controls for cytosolic and mitochondrial proteins. Stress-induced cleavage of OPA1 and C11orf83 was observed by antibodies against OPA1 and the V5 tag. ROS, reactive oxygen species. (B) Analysis of the sensitivity of CCCP-induced cleavage of C11orf83 to various protease inhibitors. HeLa cells expressing C11orf83-V5 were incubated with CCCP with or without protease inhibitors. Cells lysates were fractionated by SDS-PAGE and analyzed by immunoblotting. β-Catenin and SDHB were used as loading controls. The prevention of C11orf83-V5 proteolysis was monitored thanks to an antibody against the V5 tag. (C) OMA1 involvement in the stress-induced cleavage of C11orf83. HeLa cells were transfected with C11orf83-V5 and treated with control siRNA or siRNA against OMA1. Forty-eight hours after siRNA transfection, cells were incubated with or without CCCP and cell lysates were analyzed by immunoblotting, after SDS-PAGE, using antibodies against OMA1, OPA1, and the V5 tag. GAPDH was used as a loading control. The two bands detected by the antibody against OPA1 in the absence of CCCP correspond to short and long OPA1 isoforms. The degradation of endogenous C11orf83 under CCCP treatment (lane 3) was prevented by the depletion of OMA1 (lane 4).
    Igg Control Sepharose Bead Conjugate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg control sepharose bead conjugate/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg control sepharose bead conjugate - by Bioz Stars, 2023-03
    96/100 stars
    		  Buy from Supplier
    anti mouse igg fab2 alexa fluor 488  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc anti mouse igg fab2 alexa fluor 488
    C11orf83, like OPA1, is cleaved by <t>OMA1</t> under ΔΨ loss. (A) Analysis of the stress-induced proteolysis of C11orf83 compared to OPA1. HeLa cells expressing C11orf83-V5 were treated with mitochondrion-damaging agents for 3 h, and cell lysates were analyzed by immunoblotting after SDS-PAGE. β-Catenin and SDHB were used as loading controls for cytosolic and mitochondrial proteins. Stress-induced cleavage of OPA1 and C11orf83 was observed by antibodies against OPA1 and the V5 tag. ROS, reactive oxygen species. (B) Analysis of the sensitivity of CCCP-induced cleavage of C11orf83 to various protease inhibitors. HeLa cells expressing C11orf83-V5 were incubated with CCCP with or without protease inhibitors. Cells lysates were fractionated by SDS-PAGE and analyzed by immunoblotting. β-Catenin and SDHB were used as loading controls. The prevention of C11orf83-V5 proteolysis was monitored thanks to an antibody against the V5 tag. (C) OMA1 involvement in the stress-induced cleavage of C11orf83. HeLa cells were transfected with C11orf83-V5 and treated with control siRNA or siRNA against OMA1. Forty-eight hours after siRNA transfection, cells were incubated with or without CCCP and cell lysates were analyzed by immunoblotting, after SDS-PAGE, using antibodies against OMA1, OPA1, and the V5 tag. GAPDH was used as a loading control. The two bands detected by the antibody against OPA1 in the absence of CCCP correspond to short and long OPA1 isoforms. The degradation of endogenous C11orf83 under CCCP treatment (lane 3) was prevented by the depletion of OMA1 (lane 4).
    Anti Mouse Igg Fab2 Alexa Fluor 488, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse igg fab2 alexa fluor 488/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse igg fab2 alexa fluor 488 - by Bioz Stars, 2023-03
    96/100 stars
    		  Buy from Supplier
    pf3 igg  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc pf3 igg
    Biomarkers used in the study*
    Pf3 Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pf3 igg/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pf3 igg - by Bioz Stars, 2023-03
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    C11orf83, like OPA1, is cleaved by OMA1 under ΔΨ loss. (A) Analysis of the stress-induced proteolysis of C11orf83 compared to OPA1. HeLa cells expressing C11orf83-V5 were treated with mitochondrion-damaging agents for 3 h, and cell lysates were analyzed by immunoblotting after SDS-PAGE. β-Catenin and SDHB were used as loading controls for cytosolic and mitochondrial proteins. Stress-induced cleavage of OPA1 and C11orf83 was observed by antibodies against OPA1 and the V5 tag. ROS, reactive oxygen species. (B) Analysis of the sensitivity of CCCP-induced cleavage of C11orf83 to various protease inhibitors. HeLa cells expressing C11orf83-V5 were incubated with CCCP with or without protease inhibitors. Cells lysates were fractionated by SDS-PAGE and analyzed by immunoblotting. β-Catenin and SDHB were used as loading controls. The prevention of C11orf83-V5 proteolysis was monitored thanks to an antibody against the V5 tag. (C) OMA1 involvement in the stress-induced cleavage of C11orf83. HeLa cells were transfected with C11orf83-V5 and treated with control siRNA or siRNA against OMA1. Forty-eight hours after siRNA transfection, cells were incubated with or without CCCP and cell lysates were analyzed by immunoblotting, after SDS-PAGE, using antibodies against OMA1, OPA1, and the V5 tag. GAPDH was used as a loading control. The two bands detected by the antibody against OPA1 in the absence of CCCP correspond to short and long OPA1 isoforms. The degradation of endogenous C11orf83 under CCCP treatment (lane 3) was prevented by the depletion of OMA1 (lane 4).

    Journal: Molecular and Cellular Biology

    Article Title: C11orf83, a Mitochondrial Cardiolipin-Binding Protein Involved in bc 1 Complex Assembly and Supercomplex Stabilization

    doi: 10.1128/MCB.01047-14

    Figure Lengend Snippet: C11orf83, like OPA1, is cleaved by OMA1 under ΔΨ loss. (A) Analysis of the stress-induced proteolysis of C11orf83 compared to OPA1. HeLa cells expressing C11orf83-V5 were treated with mitochondrion-damaging agents for 3 h, and cell lysates were analyzed by immunoblotting after SDS-PAGE. β-Catenin and SDHB were used as loading controls for cytosolic and mitochondrial proteins. Stress-induced cleavage of OPA1 and C11orf83 was observed by antibodies against OPA1 and the V5 tag. ROS, reactive oxygen species. (B) Analysis of the sensitivity of CCCP-induced cleavage of C11orf83 to various protease inhibitors. HeLa cells expressing C11orf83-V5 were incubated with CCCP with or without protease inhibitors. Cells lysates were fractionated by SDS-PAGE and analyzed by immunoblotting. β-Catenin and SDHB were used as loading controls. The prevention of C11orf83-V5 proteolysis was monitored thanks to an antibody against the V5 tag. (C) OMA1 involvement in the stress-induced cleavage of C11orf83. HeLa cells were transfected with C11orf83-V5 and treated with control siRNA or siRNA against OMA1. Forty-eight hours after siRNA transfection, cells were incubated with or without CCCP and cell lysates were analyzed by immunoblotting, after SDS-PAGE, using antibodies against OMA1, OPA1, and the V5 tag. GAPDH was used as a loading control. The two bands detected by the antibody against OPA1 in the absence of CCCP correspond to short and long OPA1 isoforms. The degradation of endogenous C11orf83 under CCCP treatment (lane 3) was prevented by the depletion of OMA1 (lane 4).

    Article Snippet: Rabbit polyclonal antibodies obtained from commercial sources were anti-FIS1 (sc-98900) and anti-SIRT3 (sc-99143) from Santa Cruz Biotechnology (Santa Cruz, CA), anti-COX4 from Cell Signaling Technology (Danvers, MA), anti-OMA1 (NBP1-56970) from Novus Biological (Littleton, CO), anti-β-catenin (C2206) from Sigma-Aldrich, and anti-DIABLO (ADI-905-244) from Enzo Life Sciences.

    Techniques: Expressing, Western Blot, SDS Page, Incubation, Transfection

    Biomarkers used in the study*

    Journal: JNCI Journal of the National Cancer Institute

    Article Title: Quantitative Assessment of Effect of Preanalytic Cold Ischemic Time on Protein Expression in Breast Cancer Tissues

    doi: 10.1093/jnci/djs438

    Figure Lengend Snippet: Biomarkers used in the study*

    Article Snippet: Beta-tubulin (TUBB) , Rabbit , pF3/IgG , 2128 , Cell Signaling Technology.

    Techniques: Diagnostic Assay, Modification, Purification