Journal: Cell Death & Disease

Article Title: SKIL facilitates tumorigenesis and immune escape of NSCLC via upregulating TAZ/autophagy axis

doi: 10.1038/s41419-020-03200-7

Figure Lengend Snippet: a Western blot analysis showed that SKIL silencing decreased expression levels of autophagy markers (LC3, p62, and Beclin-1). b , c Immunofluorescence staining indicated significant decrease of autophagosome after SKIL silencing in both CALU-3 and NCI-H520 cell lines. Percentage of cells with LC3 vacuoles were counted and calculated from 100 cells in 20 fields d Western blot analysis showed that SKIL silencing decreased expression of TAZ and its downstream signaling factors (CTGF, CYR61). e , f Cycloheximide treatment resulted in faster TAZ protein degradation in SKIL-silenced CALU-3 (shSKIL) compared to control (shNC). g Co-immunoprecipitation assay showed that SKIL could bind to elements of Hippo complex (LATS2, Sav), but not TAZ. h Western blot analysis showed that overexpression of LATS2 led to increased phosphorylation of TAZ (p-TAZ) and decreased levels of TAZ. Further overexpression of SKIL reversed the effect of LATS2 overexpression on levels of p-TAZ and TAZ, without influencing LATS2 level. * P < 0.05, ** P < 0.01. Experiments were performed in triplicate.

Article Snippet: Proteins were then stained at 4 °C overnight with specific primary antibody: SNAIL1 (#3895, CST), SLUG (#9585, CST), E-cadherin (#14472, CST), vimentin (#3932, CST), GAPDH (#97166, CST), STING (#13647, CST), TBK1 (#3504, CST), p-TBK1 (#5483, CST), IRF3 (#4302, CST), p-IRF3 (#29047, CST), β-actin (#58169, CST), Beclin-1 (#3738, CST), p62 (#39749, CST), LC3-I (#4599, CST), LC3-II (#2775, CST), TAZ (#4883, CST), CTGF (#10095, CST), CYR61 (#39382, CST), LATS2 (#5888, CST), Sav (#13301, CST), or p-TAZ (#59971, CST).

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Co-Immunoprecipitation Assay, Over Expression

Journal: ACS Omega

Article Title: Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher

doi: 10.1021/acsomega.8b00635

Figure Lengend Snippet: Selective cleavage of Pyro-PtdEtn-QSY by PC-PLC in a buffered solution compared to several different phospholipases: PC-PLC, SMase, sPLA 2 (IB porcine), PLD, and buffer 140 mM NaCl–10 mM HEPES, pH 7.4 (control). No positive control was used.

Article Snippet: When the initial Pyro-PtdEtn-BHQ activatable probe was developed, it required the use of a BOC-protected phosphatidylethanolamine (LysoPtdEtn-BOC, see Scheme ).

Techniques: Positive Control

Journal: ACS Omega

Article Title: Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher

doi: 10.1021/acsomega.8b00635

Figure Lengend Snippet: Broad-range fluorescence experiments of selective cleavage of Pyro-PtdEtn-QSY by a buffered solution 140 mM NaCl–10 mM HEPES, pH 7.4 (control), and several different phospholipase isoforms (sPLA 2 (IB porcine), PLD, and PC-PLC). No positive control was used.

Article Snippet: When the initial Pyro-PtdEtn-BHQ activatable probe was developed, it required the use of a BOC-protected phosphatidylethanolamine (LysoPtdEtn-BOC, see Scheme ).

Techniques: Fluorescence, Positive Control