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    Cell Signaling Technology Inc human lc3i ii
    T. gondii and Rottlerin induce morphological and biochemical alterations associated with autophagy in BeWo Cells. Left panel: control-vehicle treated cultures ( A , B and C ); 5 µM Rottlerin-treated cultures ( D , E and F ); T. gondii infected cultures ( G , H and I ); T. gondii infected and 5 µM Rottlerin treated cultured ( L , M , N and O ). Arrows: cytoplasmic vacuoles; star: T. gondii ; double arrow head: expansion of the rough endoplasmic reticulum; arrow head: double membraned vacuoles ( P and Q ): representative western blot and densitometric analysis performed on three independent experiments of <t>LC3I/II</t> ( P ) and p62 ( Q ) proteins in BeWo cells after infection and treatment with 5 µM Rottlerin. *p < 0.05; # p < 0.01. ( R ): representative western blot of three independent experiments of PARP proteins in BeWo cells after infection and treatment with 5 µM Rottlerin. ( S ): viability of BeWo cells after infection and treatment with 5 µM Rottlerin (Rt). ( A ) Bar = 5 µm; ( B , C , F and I ), Bar = 500 nm; ( D , L and M ), Bar = 2 µm; ( E , G , H ), Bar = 1 µm.
    Human Lc3i Ii, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lc3i ii/product/Cell Signaling Technology Inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    85
    ATCC holdeman
    T. gondii and Rottlerin induce morphological and biochemical alterations associated with autophagy in BeWo Cells. Left panel: control-vehicle treated cultures ( A , B and C ); 5 µM Rottlerin-treated cultures ( D , E and F ); T. gondii infected cultures ( G , H and I ); T. gondii infected and 5 µM Rottlerin treated cultured ( L , M , N and O ). Arrows: cytoplasmic vacuoles; star: T. gondii ; double arrow head: expansion of the rough endoplasmic reticulum; arrow head: double membraned vacuoles ( P and Q ): representative western blot and densitometric analysis performed on three independent experiments of <t>LC3I/II</t> ( P ) and p62 ( Q ) proteins in BeWo cells after infection and treatment with 5 µM Rottlerin. *p < 0.05; # p < 0.01. ( R ): representative western blot of three independent experiments of PARP proteins in BeWo cells after infection and treatment with 5 µM Rottlerin. ( S ): viability of BeWo cells after infection and treatment with 5 µM Rottlerin (Rt). ( A ) Bar = 5 µm; ( B , C , F and I ), Bar = 500 nm; ( D , L and M ), Bar = 2 µm; ( E , G , H ), Bar = 1 µm.
    Holdeman, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/holdeman/product/ATCC
    Average 85 stars, based on 1 article reviews
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    93
    ATCC cluster ii
    T. gondii and Rottlerin induce morphological and biochemical alterations associated with autophagy in BeWo Cells. Left panel: control-vehicle treated cultures ( A , B and C ); 5 µM Rottlerin-treated cultures ( D , E and F ); T. gondii infected cultures ( G , H and I ); T. gondii infected and 5 µM Rottlerin treated cultured ( L , M , N and O ). Arrows: cytoplasmic vacuoles; star: T. gondii ; double arrow head: expansion of the rough endoplasmic reticulum; arrow head: double membraned vacuoles ( P and Q ): representative western blot and densitometric analysis performed on three independent experiments of <t>LC3I/II</t> ( P ) and p62 ( Q ) proteins in BeWo cells after infection and treatment with 5 µM Rottlerin. *p < 0.05; # p < 0.01. ( R ): representative western blot of three independent experiments of PARP proteins in BeWo cells after infection and treatment with 5 µM Rottlerin. ( S ): viability of BeWo cells after infection and treatment with 5 µM Rottlerin (Rt). ( A ) Bar = 5 µm; ( B , C , F and I ), Bar = 500 nm; ( D , L and M ), Bar = 2 µm; ( E , G , H ), Bar = 1 µm.
    Cluster Ii, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cluster ii/product/ATCC
    Average 93 stars, based on 1 article reviews
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    Standard format Plasmid sent in bacteria as agar stab
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    T. gondii and Rottlerin induce morphological and biochemical alterations associated with autophagy in BeWo Cells. Left panel: control-vehicle treated cultures ( A , B and C ); 5 µM Rottlerin-treated cultures ( D , E and F ); T. gondii infected cultures ( G , H and I ); T. gondii infected and 5 µM Rottlerin treated cultured ( L , M , N and O ). Arrows: cytoplasmic vacuoles; star: T. gondii ; double arrow head: expansion of the rough endoplasmic reticulum; arrow head: double membraned vacuoles ( P and Q ): representative western blot and densitometric analysis performed on three independent experiments of LC3I/II ( P ) and p62 ( Q ) proteins in BeWo cells after infection and treatment with 5 µM Rottlerin. *p < 0.05; # p < 0.01. ( R ): representative western blot of three independent experiments of PARP proteins in BeWo cells after infection and treatment with 5 µM Rottlerin. ( S ): viability of BeWo cells after infection and treatment with 5 µM Rottlerin (Rt). ( A ) Bar = 5 µm; ( B , C , F and I ), Bar = 500 nm; ( D , L and M ), Bar = 2 µm; ( E , G , H ), Bar = 1 µm.

    Journal: Scientific Reports

    Article Title: Rottlerin-mediated inhibition of Toxoplasma gondii growth in BeWo trophoblast-like cells

    doi: 10.1038/s41598-017-01525-6

    Figure Lengend Snippet: T. gondii and Rottlerin induce morphological and biochemical alterations associated with autophagy in BeWo Cells. Left panel: control-vehicle treated cultures ( A , B and C ); 5 µM Rottlerin-treated cultures ( D , E and F ); T. gondii infected cultures ( G , H and I ); T. gondii infected and 5 µM Rottlerin treated cultured ( L , M , N and O ). Arrows: cytoplasmic vacuoles; star: T. gondii ; double arrow head: expansion of the rough endoplasmic reticulum; arrow head: double membraned vacuoles ( P and Q ): representative western blot and densitometric analysis performed on three independent experiments of LC3I/II ( P ) and p62 ( Q ) proteins in BeWo cells after infection and treatment with 5 µM Rottlerin. *p < 0.05; # p < 0.01. ( R ): representative western blot of three independent experiments of PARP proteins in BeWo cells after infection and treatment with 5 µM Rottlerin. ( S ): viability of BeWo cells after infection and treatment with 5 µM Rottlerin (Rt). ( A ) Bar = 5 µm; ( B , C , F and I ), Bar = 500 nm; ( D , L and M ), Bar = 2 µm; ( E , G , H ), Bar = 1 µm.

    Article Snippet: Then, the blots were probed with polyclonal antibodies anti human LC3I/II, p4EBP-1, Beclin 1, peIF2α and PARP (Cell Signaling Technology, Boston, MA USA), SQSTM1/p62 and β-actin (Santa Cruz Biotechnology; Santa Cruz, CA) at dilution of 1:1000 overnight at 4 °C.

    Techniques: Infection, Cell Culture, Western Blot