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mklp 1 antibodies (Novus Biologicals)

Novus Biologicals mklp 1 antibodies
Mklp 1 Antibodies, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mklp 1 antibodies/product/Novus Biologicals
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99

mklp 1 antibodies - by Bioz Stars, 2023-03

85/100 stars

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dsm 17299 clinical (Clinical and Laboratory Standards Institute)

Clinical and Laboratory Standards Institute dsm 17299 clinical
Dsm 17299 Clinical, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dsm 17299 clinical/product/Clinical and Laboratory Standards Institute
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

dsm 17299 clinical - by Bioz Stars, 2023-03

86/100 stars

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destination vector plenti x1 zeo dest (Addgene inc)

Addgene inc destination vector plenti x1 zeo dest
Destination Vector Plenti X1 Zeo Dest, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/destination vector plenti x1 zeo dest/product/Addgene inc
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99

destination vector plenti x1 zeo dest - by Bioz Stars, 2023-03

91/100 stars

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anti aga (Proteintech)

Proteintech anti aga
$(a) Western blot analysis of the wild-type (WT) and mutated <t>UGT1A1,</t> <t>CTSK</t> and <t>AGA,</t> transiently expressed in HeLa Tet-On cells. Expression of the secreted proteins AGA and CTSK was evaluated in the cell lysates, secreted fractions (media) and total samples. Although UGT1A1 is translocated into ER, it is not released into the medium and remained bound through a membrane anchor to a membrane, thus UGT1A1 was analyzed in the cell lysates only. AGA is observed in several forms on the Western blots because it is cleaved during maturation. Actin and tubulin proteins from the cell lysate samples were used as loading controls. (b) Expression of the WT and mutated UGT1A1 and AGA proteins in HeLa Tet-On cells was analyzed by immunofluorescence (green). Blue is a DAPI staining.$
Anti Aga, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti aga/product/Proteintech
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99

anti aga - by Bioz Stars, 2023-03

91/100 stars

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Image Search Results

$(a) Western blot analysis of the wild-type (WT) and mutated UGT1A1, CTSK and AGA, transiently expressed in HeLa Tet-On cells. Expression of the secreted proteins AGA and CTSK was evaluated in the cell lysates, secreted fractions (media) and total samples. Although UGT1A1 is translocated into ER, it is not released into the medium and remained bound through a membrane anchor to a membrane, thus UGT1A1 was analyzed in the cell lysates only. AGA is observed in several forms on the Western blots because it is cleaved during maturation. Actin and tubulin proteins from the cell lysate samples were used as loading controls. (b) Expression of the WT and mutated UGT1A1 and AGA proteins in HeLa Tet-On cells was analyzed by immunofluorescence (green). Blue is a DAPI staining.$

Journal: Journal of molecular biology

Article Title: Silencing of aberrant secretory protein expression by disease-associated mutations

doi: 10.1016/j.jmb.2019.05.011

Figure Lengend Snippet: (a) Western blot analysis of the wild-type (WT) and mutated UGT1A1, CTSK and AGA, transiently expressed in HeLa Tet-On cells. Expression of the secreted proteins AGA and CTSK was evaluated in the cell lysates, secreted fractions (media) and total samples. Although UGT1A1 is translocated into ER, it is not released into the medium and remained bound through a membrane anchor to a membrane, thus UGT1A1 was analyzed in the cell lysates only. AGA is observed in several forms on the Western blots because it is cleaved during maturation. Actin and tubulin proteins from the cell lysate samples were used as loading controls. (b) Expression of the WT and mutated UGT1A1 and AGA proteins in HeLa Tet-On cells was analyzed by immunofluorescence (green). Blue is a DAPI staining.

Article Snippet: The antibodies used in this study: anti-SRP54 (mouse monoclonal, catalog number 610940) was from BD Bioscience, β-actin (mouse monoclonal, catalog number 66009–1-Ig), anti-AGA (rabbit polyclonal, catalog number 17299–1-AP), anti-CTSK (rabbit polyclonal, catalog number 11239–1-AP), anti-UGT1A1 (rabbit polyclonal, catalog number 23495–1-AP) were from Proteintech Group Inc., peroxidase-conjugated goat anti-mouse and (Jackson ImmunoResearch Laboratories), ECL anti-rabbit IgG, horseradish peroxidase linked whole antibody (from donkey, GE Healthcare, catalog number NA934V).

Techniques: Western Blot, Expressing, Immunofluorescence, Staining

Effect of proteasome inhibitor, MG-132, on WT and mutant proteins expression was studied. HeLa Tet-On cells were transfected with the plasmids expressing WT and mutated CTSK, AGA and UGT1A1 proteins. Cells were grown for 20 h after plasmid DNA transfection and then treated with 10 μM MG-132 (+) or DMSO (−) for 8 h before samples collection. Expression of UGT1A1 (a) was examined in the cell lysates only (it is not released into the medium), while CTSK (b) and AGA (c) were tested in the cells and media (secreted proteins) by Western blot. Actin and tubulin proteins from the cell lysates were used as loading controls.

Journal: Journal of molecular biology

Article Title: Silencing of aberrant secretory protein expression by disease-associated mutations

doi: 10.1016/j.jmb.2019.05.011

Figure Lengend Snippet: Effect of proteasome inhibitor, MG-132, on WT and mutant proteins expression was studied. HeLa Tet-On cells were transfected with the plasmids expressing WT and mutated CTSK, AGA and UGT1A1 proteins. Cells were grown for 20 h after plasmid DNA transfection and then treated with 10 μM MG-132 (+) or DMSO (−) for 8 h before samples collection. Expression of UGT1A1 (a) was examined in the cell lysates only (it is not released into the medium), while CTSK (b) and AGA (c) were tested in the cells and media (secreted proteins) by Western blot. Actin and tubulin proteins from the cell lysates were used as loading controls.

Techniques: Mutagenesis, Expressing, Transfection, Plasmid Preparation, Western Blot

Experiments were conducted with AGA (a) and CTSK (b) proteins with mutations in the hydrophobic core (h-region), and LIPA (c) with the mutation in the signal sequence c-region. HeLa Tet-On cells were transfected with plasmids expressing WT and mutated proteins and cultivated for 20 h to ensure that sufficient levels of the mutant mRNAs still remained at the beginning of the experiment (at point “0”). Then actinomycin D, an inhibitor of transcription, was added, and incubation was continued for indicated periods of time. mRNA levels were measured by RT-qPCR at each point and presented relatively to the mRNA level in the point zero for each construct. Error bars are standard errors (n = 3). Trendline was used to present dynamic of the mRNA level changes during actinomycin D treatment. Black symbols indicate wild-type (WT) and red symbols indicate mutants.

Journal: Journal of molecular biology

Article Title: Silencing of aberrant secretory protein expression by disease-associated mutations

doi: 10.1016/j.jmb.2019.05.011

Figure Lengend Snippet: Experiments were conducted with AGA (a) and CTSK (b) proteins with mutations in the hydrophobic core (h-region), and LIPA (c) with the mutation in the signal sequence c-region. HeLa Tet-On cells were transfected with plasmids expressing WT and mutated proteins and cultivated for 20 h to ensure that sufficient levels of the mutant mRNAs still remained at the beginning of the experiment (at point “0”). Then actinomycin D, an inhibitor of transcription, was added, and incubation was continued for indicated periods of time. mRNA levels were measured by RT-qPCR at each point and presented relatively to the mRNA level in the point zero for each construct. Error bars are standard errors (n = 3). Trendline was used to present dynamic of the mRNA level changes during actinomycin D treatment. Black symbols indicate wild-type (WT) and red symbols indicate mutants.

Techniques: Mutagenesis, Sequencing, Transfection, Expressing, Incubation, Quantitative RT-PCR, Construct

Effects of SRP54 knockdown on expression of secretory proteins are shown. HeLa Tet-On cells were transfected with siRNA for SRP54 to knockdown SRP54 prior to the second transfection with the plasmids expressing secretory proteins. (a) mRNA levels of the secretory proteins in SRP54 knockdown cells as measured by RT-qPCR and shown as mean values relatively to those in control cells (n = 2–4). Corresponding mRNA level in control cells is marked by red dash line. SRP54 mRNA level in SRP54 knockdown and control cells is shown in inset (n = 14). Errors bars are standard errors. (b) Protein expression of secretory proteins in SRP54 knockdown cells. CTSK and AGA expression was analyzed in the cell lysates and media, and UGT1A1 was tested in the cell lysates only (it is associated with plasma membrane) by Western blot. Actin and tubulin proteins were used as loading controls. SRP54 depletion was confirmed by Western blot. (c) Expression of SRP54 (red), UGT1A1 (green) and AGA (green) in HeLa Tet-On cells was detected by immunofluorescence. Blue is a DAPI staining.

Journal: Journal of molecular biology

Article Title: Silencing of aberrant secretory protein expression by disease-associated mutations

doi: 10.1016/j.jmb.2019.05.011

Figure Lengend Snippet: Effects of SRP54 knockdown on expression of secretory proteins are shown. HeLa Tet-On cells were transfected with siRNA for SRP54 to knockdown SRP54 prior to the second transfection with the plasmids expressing secretory proteins. (a) mRNA levels of the secretory proteins in SRP54 knockdown cells as measured by RT-qPCR and shown as mean values relatively to those in control cells (n = 2–4). Corresponding mRNA level in control cells is marked by red dash line. SRP54 mRNA level in SRP54 knockdown and control cells is shown in inset (n = 14). Errors bars are standard errors. (b) Protein expression of secretory proteins in SRP54 knockdown cells. CTSK and AGA expression was analyzed in the cell lysates and media, and UGT1A1 was tested in the cell lysates only (it is associated with plasma membrane) by Western blot. Actin and tubulin proteins were used as loading controls. SRP54 depletion was confirmed by Western blot. (c) Expression of SRP54 (red), UGT1A1 (green) and AGA (green) in HeLa Tet-On cells was detected by immunofluorescence. Blue is a DAPI staining.

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot, Immunofluorescence, Staining

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