Journal: Cancer cell

Article Title: Therapeutic Targeting of RNA Splicing Catalysis through Inhibition of Protein Arginine Methylation

doi: 10.1016/j.ccell.2019.07.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: After lyophilisation, each concatenated fraction was dissolved in 250 μl of 1x immuno-Affinity Purification Buffer (IAP buffer, #9993, Cell Signaling Technologies, CST) and subjected to two consecutive steps of methyl-R-peptides enrichment using the SDMA antibody-conjugated beads (PTMScan [sdme-R] Kit #13563, Cell Signaling Technologies) and the MMA antibody-conjugated beads (PTMScan Mono-Methyl Arginine Motif [mme-RG] Kit #12235, Cell Signaling Technologies), following the manufacturer’s instructions.

Techniques: Recombinant, Luminescence Assay, MTS Assay, Knock-In, Transgenic Assay, Sequencing, Software

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms

doi: 10.1186/s12958-022-00992-5

Figure Lengend Snippet: NR4A1 localization in the ovaries as determined by immunohistochemistry. (A)Within the ovary, positive immunoreactive signals of NR4A1 were visualized as brown stains and the nucleus as blue stains (original magnification of 200X and 400X, scale bar = 120 µm and 60 µm). POI: premature ovarian insufficiency, hUCMSCs: human umbilical cord mesenchymal stem cells.*: P < 0.05

Article Snippet: The primary antibodies Cyp17a1 (1:100 dilution; ab125022; Abcam), FSHR (1:100 dilution; bs-20658R; Bioss), and NR4A1 (1:50 dilution; 12,235–1-AP; Proteintech) used in these reactions were incubated at 4℃ overnight with the second antibody (HRP or fluorescence) being added on the following day.

Techniques: Immunohistochemistry

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms

doi: 10.1186/s12958-022-00992-5

Figure Lengend Snippet: NR4A1 localization in TICs as measured using immunofluorescence. Expression of NR4A1, as well as phospho-NR4A1 levels in each group, were assessed by western blot. A Positive immunoreactive NR4A1 signals within the TICs were visualized as red staining and the nucleus as blue staining (original magnification of 400X, scale bar = 30 µm). B - H The representative bands of western blot are shown in the form of histograms in the co-cultured hUCMSCs and cisplatin-treated TICs. CDDP, cisplatin. *: P < 0.05, **: P < 0.01

Article Snippet: The primary antibodies Cyp17a1 (1:100 dilution; ab125022; Abcam), FSHR (1:100 dilution; bs-20658R; Bioss), and NR4A1 (1:50 dilution; 12,235–1-AP; Proteintech) used in these reactions were incubated at 4℃ overnight with the second antibody (HRP or fluorescence) being added on the following day.

Techniques: Immunofluorescence, Expressing, Western Blot, Staining, Cell Culture

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms

doi: 10.1186/s12958-022-00992-5

Figure Lengend Snippet: Expression of NR4A1, Bcl-2, Cytc, Bax, caspase-9, caspase-3, and the ratio of Bcl-2/Bax levels are present as the original bands and histograms in TICs following cisplatin treatment, hUCMSCs supernatant co-culture and the antagonist of NR4A1 DIM-C-pPhOH. Statistics analysis of these data is indicated within the histograms. **: P < 0.01.*: P < 0.05

Article Snippet: The primary antibodies Cyp17a1 (1:100 dilution; ab125022; Abcam), FSHR (1:100 dilution; bs-20658R; Bioss), and NR4A1 (1:50 dilution; 12,235–1-AP; Proteintech) used in these reactions were incubated at 4℃ overnight with the second antibody (HRP or fluorescence) being added on the following day.

Techniques: Expressing, Co-Culture Assay