Article Title: Minichromosome maintenance protein 2 and 3 promote osteosarcoma progression via DHX9 and predict poor patient prognosis
Figure Lengend Snippet: ( A ) The silver staining for proteins separated by SDS-PAGE after IgG, MCM2, or MCM3 pull-down in 293T cells. ( B ) Whole-cell lysates were immunoprecipitated with the anti-MCM2 antibody or anti-MCM3 antibody followed by immunoblotting with anti-MCM2, MCM3, and DHX9 antibodies in the 293T cell line and the indicated osteosarcoma cell lines. IgG was used as a negative control. ( C) Whole-cell lysates were immunoprecipitated with the anti-DHX9 antibody followed by immunoblotting with anti-MCM2, MCM3, and DHX9 antibodies in the MNNG/HOS cell line. IgG was used as a negative control. ( D ) An immunofluorescence study was performed using anti-MCM2, MCM3, and DHX9 antibodies in the MNNG/HOS cell line. DAPI was used as a control for nuclear staining.
Article Snippet: Each slide was blocked for endogenous peroxidase activity by incubation in 0.3% H 2 O 2 for 10 min, and then incubated at 37°C with a 1:100 dilution of the polyclonal antibody against MCM2 (Bioworld, Nanjing, China), MCM3 (1:300, Bioworld), DHX9 (1:200, Proteintech), or Ki-67 (1:200, DAKO).
Techniques: Silver Staining, SDS Page, Immunoprecipitation, Western Blot, Negative Control, Immunofluorescence, Staining