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  • 96
    ATCC ma 104 cells
    Utilization of nsp2 to express foreign gene. Viable GFP recombinant virus was generated in <t>MA-104</t> cells. (A and B) Light (A) and fluorescence (B) (×10) microscopy under the same field showed the fluorescence was associated with the virus-infected
    Ma 104 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher carbopac ma 1 column
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Carbopac Ma 1 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Siemens AG ma pitch effective ma
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Ma Pitch Effective Ma, supplied by Siemens AG, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Corning Life Sciences bellavance ma
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Bellavance Ma, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Trinity Biotech bellgrove ma
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Bellgrove Ma, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Theratechnologies ma thera
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Ma Thera, supplied by Theratechnologies, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    RStudio ma url
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Ma Url, supplied by RStudio, used in various techniques. Bioz Stars score: 91/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore meo2 ma
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Meo2 Ma, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Dwyer Instruments yialamas ma
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Yialamas Ma, supplied by Dwyer Instruments, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Tocris ma2029
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Ma2029, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Greiner Bio contreras ma
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Contreras Ma, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 88/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    contreras ma - by Bioz Stars, 2020-08
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    92
    Trinity Biotech geer ma
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Geer Ma, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Schiff Nutrition International heller ma
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Heller Ma, supplied by Schiff Nutrition International, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore ma hydrochloride
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Ma Hydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher ma † † † † supersignal
    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) <t>CD31</t> antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.
    Ma † † † † Supersignal, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abcam npm1 ma
    INPP4B is upregulated by <t>NPM1-mA</t> in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
    Npm1 Ma, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies rcc ma
    INPP4B is upregulated by <t>NPM1-mA</t> in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
    Rcc Ma, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Prantner GmbH glicksman ma
    INPP4B is upregulated by <t>NPM1-mA</t> in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p
    Glicksman Ma, supplied by Prantner GmbH, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Utilization of nsp2 to express foreign gene. Viable GFP recombinant virus was generated in MA-104 cells. (A and B) Light (A) and fluorescence (B) (×10) microscopy under the same field showed the fluorescence was associated with the virus-infected

    Journal:

    Article Title: Identification of Nonessential Regions of the nsp2 Replicase Protein of Porcine Reproductive and Respiratory Syndrome Virus Strain VR-2332 for Replication in Cell Culture ▿

    doi: 10.1128/JVI.00562-07

    Figure Lengend Snippet: Utilization of nsp2 to express foreign gene. Viable GFP recombinant virus was generated in MA-104 cells. (A and B) Light (A) and fluorescence (B) (×10) microscopy under the same field showed the fluorescence was associated with the virus-infected

    Article Snippet: MA-104 cells or cells of a subclone (MARC-145 cells; ATCC CRL-11171), an African green monkey kidney epithelial cell line which supports PRRSV replication, were maintained in Eagle's minimal essential medium (EMEM) (SAFC Biosciences) supplemented with 10% fetal bovine serum at 37°C with 5% CO2 .

    Techniques: Recombinant, Generated, Fluorescence, Microscopy, Infection

    Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) CD31 antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.

    Journal: Biotechnology journal

    Article Title: Mechanotransduction Effects on Endothelial Cell Proliferation via CD31 and VEGFR2: Implications for Immunomagnetic Separation

    doi: 10.1002/biot.201600750

    Figure Lengend Snippet: Effects of unconjugated beads (B) and free antibody (Ab) on HUVEC proliferation for ( A ) VEGFR2 and ( B ) CD31 antibodies. No statistically significant difference in cell proliferation was observed following exposure to unconjugated beads, antibodies, or their combination at a significance level α=0.05. Starting sample size=10,000 cells, N ≥ 3 for each setting.

    Article Snippet: Anti-Biotin MACSi bead particles (Cat No. 130-092-357, Miltenyi Biotec) were conjugated with Biotinylated CD309 (VEGFR2/KDR) (Cat No. 130-093-603, Miltenyi Biotec) or CD31 (Cat No. MA1-19510, Thermo Scientific Pierce Antibodies) antibodies through avidin-biotin interactions.

    Techniques:

    Cell proliferation in 2D culture versus bead to cell ratio in the absence or presence of a magnetic field for ( A ) VEGFR2 targeting beads exposed to HUVECs, ( B ) VEGFR2 targeting beads exposed to KDR cells. No statistically significant differences were seen in the cell proliferation of HUVECs or KDR cells conjugated with VEGR2 targeting beads. ( C ) CD31 targeting beads exposed to HUVECs. There was a statistically significant difference in cell proliferation of HUVECs conjugated with CD31 targeting beads in the absence or presence of magnetic field (p

    Journal: Biotechnology journal

    Article Title: Mechanotransduction Effects on Endothelial Cell Proliferation via CD31 and VEGFR2: Implications for Immunomagnetic Separation

    doi: 10.1002/biot.201600750

    Figure Lengend Snippet: Cell proliferation in 2D culture versus bead to cell ratio in the absence or presence of a magnetic field for ( A ) VEGFR2 targeting beads exposed to HUVECs, ( B ) VEGFR2 targeting beads exposed to KDR cells. No statistically significant differences were seen in the cell proliferation of HUVECs or KDR cells conjugated with VEGR2 targeting beads. ( C ) CD31 targeting beads exposed to HUVECs. There was a statistically significant difference in cell proliferation of HUVECs conjugated with CD31 targeting beads in the absence or presence of magnetic field (p

    Article Snippet: Anti-Biotin MACSi bead particles (Cat No. 130-092-357, Miltenyi Biotec) were conjugated with Biotinylated CD309 (VEGFR2/KDR) (Cat No. 130-093-603, Miltenyi Biotec) or CD31 (Cat No. MA1-19510, Thermo Scientific Pierce Antibodies) antibodies through avidin-biotin interactions.

    Techniques:

    PBMC and HUVEC proliferation (CyQuant assay fluorescence intensity, normalized to control = 1) exposed to VEGFR2 targeting or CD31-targeting beads via a magnetic flow through sorter (short exposure) or a 2D permanent magnetic platform (long exposure): ( A ) HUVECs, ( B ) PBMCs. No statistical differences in proliferation were observed between the cell sorter and the 2D plate for either antibody in both cell lines. Starting sample size=10,000 cells, N ≥ 3 for each setting.

    Journal: Biotechnology journal

    Article Title: Mechanotransduction Effects on Endothelial Cell Proliferation via CD31 and VEGFR2: Implications for Immunomagnetic Separation

    doi: 10.1002/biot.201600750

    Figure Lengend Snippet: PBMC and HUVEC proliferation (CyQuant assay fluorescence intensity, normalized to control = 1) exposed to VEGFR2 targeting or CD31-targeting beads via a magnetic flow through sorter (short exposure) or a 2D permanent magnetic platform (long exposure): ( A ) HUVECs, ( B ) PBMCs. No statistical differences in proliferation were observed between the cell sorter and the 2D plate for either antibody in both cell lines. Starting sample size=10,000 cells, N ≥ 3 for each setting.

    Article Snippet: Anti-Biotin MACSi bead particles (Cat No. 130-092-357, Miltenyi Biotec) were conjugated with Biotinylated CD309 (VEGFR2/KDR) (Cat No. 130-093-603, Miltenyi Biotec) or CD31 (Cat No. MA1-19510, Thermo Scientific Pierce Antibodies) antibodies through avidin-biotin interactions.

    Techniques: CyQUANT Assay, Fluorescence, Flow Cytometry

    Magnetic bead conjugation to cells ( A ) Schematic, ( B ) microscope image of anti-CD31 MACSi beads attached to HUVECs, ( C ) Number of beads attached to HUVECs as a function of bead to cell ratio (anti-CD31 beads shown).

    Journal: Biotechnology journal

    Article Title: Mechanotransduction Effects on Endothelial Cell Proliferation via CD31 and VEGFR2: Implications for Immunomagnetic Separation

    doi: 10.1002/biot.201600750

    Figure Lengend Snippet: Magnetic bead conjugation to cells ( A ) Schematic, ( B ) microscope image of anti-CD31 MACSi beads attached to HUVECs, ( C ) Number of beads attached to HUVECs as a function of bead to cell ratio (anti-CD31 beads shown).

    Article Snippet: Anti-Biotin MACSi bead particles (Cat No. 130-092-357, Miltenyi Biotec) were conjugated with Biotinylated CD309 (VEGFR2/KDR) (Cat No. 130-093-603, Miltenyi Biotec) or CD31 (Cat No. MA1-19510, Thermo Scientific Pierce Antibodies) antibodies through avidin-biotin interactions.

    Techniques: Conjugation Assay, Microscopy

    INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: INPP4B is upregulated by NPM1-mA in leukemia cells via ERK/Ets-1 signaling. ( a ) qRT-PCR analysis of INPP4B mRNA expression, ( b ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. ( c ) qRT-PCR analysis of INPP4B mRNA expression, ( d ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the THP-1 and K562 cells transduced with the plasmids expressing NPM1-wt or NPM1-mA. ( e ) qRT-PCR analysis of INPP4B mRNA expression, ( f ) western blotting analysis of INPP4B, p-SGK3 T320 , SGK3, p-Ets-1 and Ets-1 from the OCI-AML3 cells transfected with the control siRNA or siEts-1. ( g ) Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 and SGK3, ( h ) qRT-PCR analysis of INPP4B mRNA expression from the OCI-AML3 cells treated with different concentration of PD98059 (0, 10, 20 and 40 μM). i Western blotting analysis of p-ERK, ERK, p-Ets-1, Ets-1, INPP4B, p-SGK3 T320 , SGK3 and NPM1-mA from the NPM1-mA-silenced OCI-AML3 cells. Proteins were quantified using image software and normalized against β-actin. Data were represented as mean ± s.d. of three individual experiments. * p

    Article Snippet: INPP4B is upregulated by NPM1-mA via ERK/Ets-1 signaling in leukemia cells We evaluated the potential molecular mechanism underlying INPP4B upregulation in leukemia cells with NPM1-mA.

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Transduction, Transfection, Concentration Assay, Software

    NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: NPM1-mA-mediated INPP4B upregulation promotes cell proliferation in OCI-AML3 cells. The NPM1-mA-silenced OCI-AML3 cells were subjected to ( a ) CCK8 assays and ( b ) colony forming assays. c The NPM1-mA-silenced OCI-AML3 cells were transfected with the pEAK-Flag/INPP4B plasmids, western blotting analysis of INPP4B and NPM1-mA. Proteins were quantified using image software and normalized against β-actin. d CCK-8 assay analysis of cell proliferation in NPM1-mA-silenced OCI-AML3 cells, followed by Flag-INPP4B introduction. Data were represented as mean ± s.d. of three individual experiments. * p

    Article Snippet: INPP4B is upregulated by NPM1-mA via ERK/Ets-1 signaling in leukemia cells We evaluated the potential molecular mechanism underlying INPP4B upregulation in leukemia cells with NPM1-mA.

    Techniques: Transfection, Western Blot, Software, CCK-8 Assay

    High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: High expression of INPP4B is associated with poor survival outcome in NPM1-mutated leukemia. Kaplan-Meier survival data of 153 AML patients were used to analysis ( a ) OS and ( b ) EFS curves according to INPP4B levels. c Heatmap of 38 primary AML samples with NPM1 mutation from TCGA dataset in which INPP4B expression was aligned with patient event (Survival or Death). Kaplan-Meier survival data of patients with NPM1-mutated AML were used to analysis ( d ) OS and ( e ) EFS curves according to INPP4B levels. f Schematic diagram describing the functional significance of INPP4B in the NPM1-mutated leukemia cells. INPP4B promotes leukemia cell survival in a SGK3-dependent and AKT-independent manner. The expression of INPP4B partially upregulated by NPM1-mA is due to ERK/Ets-1 signaling

    Article Snippet: INPP4B is upregulated by NPM1-mA via ERK/Ets-1 signaling in leukemia cells We evaluated the potential molecular mechanism underlying INPP4B upregulation in leukemia cells with NPM1-mA.

    Techniques: Expressing, Mutagenesis, Functional Assay

    High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia

    doi: 10.1186/s13046-018-0675-9

    Figure Lengend Snippet: High expression levels of INPP4B in leukemia cells with the NPM1 mutation. a RNA-seq mRNA expression data from the TCGA database were used to compare INPP4B expression between AML patients with ( n = 41) and without NPM1 mutation ( n = 130). * p

    Article Snippet: INPP4B is upregulated by NPM1-mA via ERK/Ets-1 signaling in leukemia cells We evaluated the potential molecular mechanism underlying INPP4B upregulation in leukemia cells with NPM1-mA.

    Techniques: Expressing, Mutagenesis, RNA Sequencing Assay