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  • 99
    ATCC ma104 cells
    Ma104 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher texas red
    Texas Red, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher micro bca assay
    Micro Bca Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher doxorubicin
    Effect of <t>doxorubicin</t> and DoxQ on adult rat cardiomyocyte viability. a Dox versus DoxQ dose response. N = 3; mean ± SEM. * P
    Doxorubicin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 696 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher phorbol 12 myristate 13 acetate
    Cytokine production of CD4 +  T cells in myasthenia gravis (MG) subgroups.  (A)  Measurement of intracellular IL-21, IL-4, IL-17A, IL-10, and IFN-γ in CD4 +  T cells of a patient and a healthy control (HC) by flow cytometry after 4 h of stimulation with phorbol 12-myristate 13-acetate and ionomycin in cell culture.  (B)  The AChR-MG ( n  = 54) patients had higher IL-21, IL-4, IL-17A, and IL-10 ( p
    Phorbol 12 Myristate 13 Acetate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 778 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Selleck Chemicals 3 methyladenine
    Cytokine production of CD4 +  T cells in myasthenia gravis (MG) subgroups.  (A)  Measurement of intracellular IL-21, IL-4, IL-17A, IL-10, and IFN-γ in CD4 +  T cells of a patient and a healthy control (HC) by flow cytometry after 4 h of stimulation with phorbol 12-myristate 13-acetate and ionomycin in cell culture.  (B)  The AChR-MG ( n  = 54) patients had higher IL-21, IL-4, IL-17A, and IL-10 ( p
    3 Methyladenine, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 99/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 3 methyladenine 3 ma
    Cytokine production of CD4 +  T cells in myasthenia gravis (MG) subgroups.  (A)  Measurement of intracellular IL-21, IL-4, IL-17A, IL-10, and IFN-γ in CD4 +  T cells of a patient and a healthy control (HC) by flow cytometry after 4 h of stimulation with phorbol 12-myristate 13-acetate and ionomycin in cell culture.  (B)  The AChR-MG ( n  = 54) patients had higher IL-21, IL-4, IL-17A, and IL-10 ( p
    3 Methyladenine 3 Ma, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mitomycin c
    Cytokine production of CD4 +  T cells in myasthenia gravis (MG) subgroups.  (A)  Measurement of intracellular IL-21, IL-4, IL-17A, IL-10, and IFN-γ in CD4 +  T cells of a patient and a healthy control (HC) by flow cytometry after 4 h of stimulation with phorbol 12-myristate 13-acetate and ionomycin in cell culture.  (B)  The AChR-MG ( n  = 54) patients had higher IL-21, IL-4, IL-17A, and IL-10 ( p
    Mitomycin C, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Janssen dragon durey ma
    Cytokine production of CD4 +  T cells in myasthenia gravis (MG) subgroups.  (A)  Measurement of intracellular IL-21, IL-4, IL-17A, IL-10, and IFN-γ in CD4 +  T cells of a patient and a healthy control (HC) by flow cytometry after 4 h of stimulation with phorbol 12-myristate 13-acetate and ionomycin in cell culture.  (B)  The AChR-MG ( n  = 54) patients had higher IL-21, IL-4, IL-17A, and IL-10 ( p
    Dragon Durey Ma, supplied by Janssen, used in various techniques. Bioz Stars score: 90/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vollrath landolt ma
    Cytokine production of CD4 +  T cells in myasthenia gravis (MG) subgroups.  (A)  Measurement of intracellular IL-21, IL-4, IL-17A, IL-10, and IFN-γ in CD4 +  T cells of a patient and a healthy control (HC) by flow cytometry after 4 h of stimulation with phorbol 12-myristate 13-acetate and ionomycin in cell culture.  (B)  The AChR-MG ( n  = 54) patients had higher IL-21, IL-4, IL-17A, and IL-10 ( p
    Landolt Ma, supplied by Vollrath, used in various techniques. Bioz Stars score: 89/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt
    Cytokine production of CD4 +  T cells in myasthenia gravis (MG) subgroups.  (A)  Measurement of intracellular IL-21, IL-4, IL-17A, IL-10, and IFN-γ in CD4 +  T cells of a patient and a healthy control (HC) by flow cytometry after 4 h of stimulation with phorbol 12-myristate 13-acetate and ionomycin in cell culture.  (B)  The AChR-MG ( n  = 54) patients had higher IL-21, IL-4, IL-17A, and IL-10 ( p
    Oligo Dt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28889 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore autophagy inhibitor 3 methyladenine
    Cytokine production of CD4 +  T cells in myasthenia gravis (MG) subgroups.  (A)  Measurement of intracellular IL-21, IL-4, IL-17A, IL-10, and IFN-γ in CD4 +  T cells of a patient and a healthy control (HC) by flow cytometry after 4 h of stimulation with phorbol 12-myristate 13-acetate and ionomycin in cell culture.  (B)  The AChR-MG ( n  = 54) patients had higher IL-21, IL-4, IL-17A, and IL-10 ( p
    Autophagy Inhibitor 3 Methyladenine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Staples ma 11 2 11 ma
    Preparation and characterization of 2D net <t>-poly(MA-11-2-11-MA).</t> ( A ) Schematic illustration of the molecular structure of gemini monomer MA-11-2-11-MA and 2D net- poly(MA-11-2-11-MA). ( B ) AFM image and height analysis of the 2D net- poly(MA-11-2-11-MA) on the silicon substrate. Rq, root mean square roughness. ( C ) Simulation of the bilayer thickness with vertically arranged MA-11-2-11-MA monomers using Materials Studio. ( D ) TEM image of the freestanding film of 2D net -poly(MA-11-2-11-MA).
    Ma 11 2 11 Ma, supplied by Staples, used in various techniques. Bioz Stars score: 94/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    ChromoTek gfp trap ma beads
    UNC93B1 association with STIM1 ER-luminal is required for STIM1 oligomerization. a Schematic representation of STIM1 full-length protein (WT) and STIM1 mutants (STIM1-ΔCt and STIM1-CAD) used in this study. b Fibroblasts expressing <t>GFP-tagged</t> STIM1- WT, −ΔCT and −CAD and UNC93B1 WT <t>-FLAG-tagged</t> plasmids were immunoprecipitated with GFP beads and immunoblotted with anti-GFP and anti-FLAG antibodies. One experiment representative out of two is shown. c STIM1 oligomerization was followed in HeLa cells in the presence of either Cherry-tagged WT or 3d-mutated UNC93B1 by measuring the increase in the FRET efficiency of YFP-tagged and CFP-tagged STIM1 upon stimulation with 1 μM TG over time. The V50 parameter is reported as the time-to-half–maximum FRET efficiency. Graph shows mean ± S.E.M. ( n = 3 experiments); * P
    Gfp Trap Ma Beads, supplied by ChromoTek, used in various techniques. Bioz Stars score: 89/100, based on 253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bio-Rad ma cm2
    UNC93B1 association with STIM1 ER-luminal is required for STIM1 oligomerization. a Schematic representation of STIM1 full-length protein (WT) and STIM1 mutants (STIM1-ΔCt and STIM1-CAD) used in this study. b Fibroblasts expressing <t>GFP-tagged</t> STIM1- WT, −ΔCT and −CAD and UNC93B1 WT <t>-FLAG-tagged</t> plasmids were immunoprecipitated with GFP beads and immunoblotted with anti-GFP and anti-FLAG antibodies. One experiment representative out of two is shown. c STIM1 oligomerization was followed in HeLa cells in the presence of either Cherry-tagged WT or 3d-mutated UNC93B1 by measuring the increase in the FRET efficiency of YFP-tagged and CFP-tagged STIM1 upon stimulation with 1 μM TG over time. The V50 parameter is reported as the time-to-half–maximum FRET efficiency. Graph shows mean ± S.E.M. ( n = 3 experiments); * P
    Ma Cm2, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher anhydrotetracycline
    UNC93B1 association with STIM1 ER-luminal is required for STIM1 oligomerization. a Schematic representation of STIM1 full-length protein (WT) and STIM1 mutants (STIM1-ΔCt and STIM1-CAD) used in this study. b Fibroblasts expressing <t>GFP-tagged</t> STIM1- WT, −ΔCT and −CAD and UNC93B1 WT <t>-FLAG-tagged</t> plasmids were immunoprecipitated with GFP beads and immunoblotted with anti-GFP and anti-FLAG antibodies. One experiment representative out of two is shown. c STIM1 oligomerization was followed in HeLa cells in the presence of either Cherry-tagged WT or 3d-mutated UNC93B1 by measuring the increase in the FRET efficiency of YFP-tagged and CFP-tagged STIM1 upon stimulation with 1 μM TG over time. The V50 parameter is reported as the time-to-half–maximum FRET efficiency. Graph shows mean ± S.E.M. ( n = 3 experiments); * P
    Anhydrotetracycline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Recombinant Rat CCL3 MIP 1 alpha Protein from R D Systems is derived from E coli The Recombinant Rat CCL3 MIP 1 alpha Protein has been validated for the
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    The Recombinant Mouse CCL3 MIP 1 alpha Protein from R D Systems is derived from E coli The Recombinant Mouse CCL3 MIP 1 alpha Protein has been validated for the
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    Test Automation System for Durability Testing
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    Image Search Results


    Effect of doxorubicin and DoxQ on adult rat cardiomyocyte viability. a Dox versus DoxQ dose response. N = 3; mean ± SEM. * P

    Journal: Drug delivery and translational research

    Article Title: Mechanistically elucidating the in vitro safety and efficacy of a novel doxorubicin derivative

    doi: 10.1007/s13346-017-0379-2

    Figure Lengend Snippet: Effect of doxorubicin and DoxQ on adult rat cardiomyocyte viability. a Dox versus DoxQ dose response. N = 3; mean ± SEM. * P

    Article Snippet: The inhibitory effects of CYP3A4 by DoxQ, doxorubicin, quercetin, or a mixture of doxorubicin and quercetin were examined using CYP Vivid kits from Life Technologies following the manufacturer’s instructions.

    Techniques:

    Fold change in P-gp ATPase activity of doxorubicin, DoxQ, a mixture of doxorubicin and quercetin, or quercetin at 1, 10, 50, 100, and 200 µM relative to basal activity. N = 4; mean ± SEM. * P

    Journal: Drug delivery and translational research

    Article Title: Mechanistically elucidating the in vitro safety and efficacy of a novel doxorubicin derivative

    doi: 10.1007/s13346-017-0379-2

    Figure Lengend Snippet: Fold change in P-gp ATPase activity of doxorubicin, DoxQ, a mixture of doxorubicin and quercetin, or quercetin at 1, 10, 50, 100, and 200 µM relative to basal activity. N = 4; mean ± SEM. * P

    Article Snippet: The inhibitory effects of CYP3A4 by DoxQ, doxorubicin, quercetin, or a mixture of doxorubicin and quercetin were examined using CYP Vivid kits from Life Technologies following the manufacturer’s instructions.

    Techniques: Activity Assay

    The cytotoxic effects of doxorubicin, a mixture of doxorubicin and quercetin, or DoxQ determined by resazurin blue assay expressed as IC 50 after 5 µM treatment for 72 h. N = 4; mean ± SD

    Journal: Drug delivery and translational research

    Article Title: Mechanistically elucidating the in vitro safety and efficacy of a novel doxorubicin derivative

    doi: 10.1007/s13346-017-0379-2

    Figure Lengend Snippet: The cytotoxic effects of doxorubicin, a mixture of doxorubicin and quercetin, or DoxQ determined by resazurin blue assay expressed as IC 50 after 5 µM treatment for 72 h. N = 4; mean ± SD

    Article Snippet: The inhibitory effects of CYP3A4 by DoxQ, doxorubicin, quercetin, or a mixture of doxorubicin and quercetin were examined using CYP Vivid kits from Life Technologies following the manufacturer’s instructions.

    Techniques:

    Fold expression of oxidative stress markers in RL-14 cells after treatment at 10 µM for 24 h of doxorubicin, DoxQ, or quercetin. The mRNA expression of a GST-A1 and b HO-1 in RL-14 cells. The mRNA expression was quantified by RT-PCR and normalized to β-actin as a housekeeping gene. N = 6; mean ± SEM. * P

    Journal: Drug delivery and translational research

    Article Title: Mechanistically elucidating the in vitro safety and efficacy of a novel doxorubicin derivative

    doi: 10.1007/s13346-017-0379-2

    Figure Lengend Snippet: Fold expression of oxidative stress markers in RL-14 cells after treatment at 10 µM for 24 h of doxorubicin, DoxQ, or quercetin. The mRNA expression of a GST-A1 and b HO-1 in RL-14 cells. The mRNA expression was quantified by RT-PCR and normalized to β-actin as a housekeeping gene. N = 6; mean ± SEM. * P

    Article Snippet: The inhibitory effects of CYP3A4 by DoxQ, doxorubicin, quercetin, or a mixture of doxorubicin and quercetin were examined using CYP Vivid kits from Life Technologies following the manufacturer’s instructions.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    A schematic representation of the release of doxorubicin and quercetin from DoxQ

    Journal: Drug delivery and translational research

    Article Title: Mechanistically elucidating the in vitro safety and efficacy of a novel doxorubicin derivative

    doi: 10.1007/s13346-017-0379-2

    Figure Lengend Snippet: A schematic representation of the release of doxorubicin and quercetin from DoxQ

    Article Snippet: The inhibitory effects of CYP3A4 by DoxQ, doxorubicin, quercetin, or a mixture of doxorubicin and quercetin were examined using CYP Vivid kits from Life Technologies following the manufacturer’s instructions.

    Techniques:

    In vitro release of doxorubicin and quercetin from DoxQ quantified by HPLC. N = 3; mean ± SEM

    Journal: Drug delivery and translational research

    Article Title: Mechanistically elucidating the in vitro safety and efficacy of a novel doxorubicin derivative

    doi: 10.1007/s13346-017-0379-2

    Figure Lengend Snippet: In vitro release of doxorubicin and quercetin from DoxQ quantified by HPLC. N = 3; mean ± SEM

    Article Snippet: The inhibitory effects of CYP3A4 by DoxQ, doxorubicin, quercetin, or a mixture of doxorubicin and quercetin were examined using CYP Vivid kits from Life Technologies following the manufacturer’s instructions.

    Techniques: In Vitro, High Performance Liquid Chromatography

    Fluorescence imaging study of cell uptake of doxorubicin by MDCK-MDR cells (P-gp positive). Cells were treated with a 50 nM free doxorubicin, b 50 nM doxorubicin and 50 nM quercetin, and c 50 nM DoxQ

    Journal: Drug delivery and translational research

    Article Title: Mechanistically elucidating the in vitro safety and efficacy of a novel doxorubicin derivative

    doi: 10.1007/s13346-017-0379-2

    Figure Lengend Snippet: Fluorescence imaging study of cell uptake of doxorubicin by MDCK-MDR cells (P-gp positive). Cells were treated with a 50 nM free doxorubicin, b 50 nM doxorubicin and 50 nM quercetin, and c 50 nM DoxQ

    Article Snippet: The inhibitory effects of CYP3A4 by DoxQ, doxorubicin, quercetin, or a mixture of doxorubicin and quercetin were examined using CYP Vivid kits from Life Technologies following the manufacturer’s instructions.

    Techniques: Fluorescence, Imaging

    Antioxidant activity of DoxQ in comparison to doxorubicin, a mixture of doxorubicin and quercetin, or quercetin alone expressed as Trolox equivalents ( N = 4; mean ± SEM). + P

    Journal: Drug delivery and translational research

    Article Title: Mechanistically elucidating the in vitro safety and efficacy of a novel doxorubicin derivative

    doi: 10.1007/s13346-017-0379-2

    Figure Lengend Snippet: Antioxidant activity of DoxQ in comparison to doxorubicin, a mixture of doxorubicin and quercetin, or quercetin alone expressed as Trolox equivalents ( N = 4; mean ± SEM). + P

    Article Snippet: The inhibitory effects of CYP3A4 by DoxQ, doxorubicin, quercetin, or a mixture of doxorubicin and quercetin were examined using CYP Vivid kits from Life Technologies following the manufacturer’s instructions.

    Techniques: Antioxidant Activity Assay

    Fold expression of cardiac hypertrophy markers in RL-14 cells after treatment at 10 µM for 24 h of doxorubicin, DoxQ, or quercetin. The mRNA expression of a BNP, b α-MHC, and c β-MHC in RL-14 cells. The mRNA expression was quantified by RT-PCR and normalized to β-actin as a housekeeping gene. N = 6; mean ± SEM.* P

    Journal: Drug delivery and translational research

    Article Title: Mechanistically elucidating the in vitro safety and efficacy of a novel doxorubicin derivative

    doi: 10.1007/s13346-017-0379-2

    Figure Lengend Snippet: Fold expression of cardiac hypertrophy markers in RL-14 cells after treatment at 10 µM for 24 h of doxorubicin, DoxQ, or quercetin. The mRNA expression of a BNP, b α-MHC, and c β-MHC in RL-14 cells. The mRNA expression was quantified by RT-PCR and normalized to β-actin as a housekeeping gene. N = 6; mean ± SEM.* P

    Article Snippet: The inhibitory effects of CYP3A4 by DoxQ, doxorubicin, quercetin, or a mixture of doxorubicin and quercetin were examined using CYP Vivid kits from Life Technologies following the manufacturer’s instructions.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Cytokine production of CD4 +  T cells in myasthenia gravis (MG) subgroups.  (A)  Measurement of intracellular IL-21, IL-4, IL-17A, IL-10, and IFN-γ in CD4 +  T cells of a patient and a healthy control (HC) by flow cytometry after 4 h of stimulation with phorbol 12-myristate 13-acetate and ionomycin in cell culture.  (B)  The AChR-MG ( n  = 54) patients had higher IL-21, IL-4, IL-17A, and IL-10 ( p

    Journal: Frontiers in Immunology

    Article Title: CD4+ T Cells of Myasthenia Gravis Patients Are Characterized by Increased IL-21, IL-4, and IL-17A Productions and Higher Presence of PD-1 and ICOS

    doi: 10.3389/fimmu.2020.00809

    Figure Lengend Snippet: Cytokine production of CD4 + T cells in myasthenia gravis (MG) subgroups. (A) Measurement of intracellular IL-21, IL-4, IL-17A, IL-10, and IFN-γ in CD4 + T cells of a patient and a healthy control (HC) by flow cytometry after 4 h of stimulation with phorbol 12-myristate 13-acetate and ionomycin in cell culture. (B) The AChR-MG ( n = 54) patients had higher IL-21, IL-4, IL-17A, and IL-10 ( p

    Article Snippet: Intracellular Staining Freshly isolated PBMCs were seeded in 48-well plates at a final concentration of 2 × 106 cells/ml in complete RPMI1640 medium supplemented with 2 mM L-glutamine, 100 IU/100 mg/ml penicillin/streptomycin (Sigma), and 10% fetal bovine serum (Gibco) and were stimulated by the cell stimulation cocktail (500X) containing phorbol 12-myristate 13-acetate and ionomycin (eBioscience, ThermoFisher) for 4 h at 37°C.

    Techniques: Flow Cytometry, Cell Culture

    Preparation and characterization of 2D net -poly(MA-11-2-11-MA). ( A ) Schematic illustration of the molecular structure of gemini monomer MA-11-2-11-MA and 2D net- poly(MA-11-2-11-MA). ( B ) AFM image and height analysis of the 2D net- poly(MA-11-2-11-MA) on the silicon substrate. Rq, root mean square roughness. ( C ) Simulation of the bilayer thickness with vertically arranged MA-11-2-11-MA monomers using Materials Studio. ( D ) TEM image of the freestanding film of 2D net -poly(MA-11-2-11-MA).

    Journal: Science Advances

    Article Title: Two-dimensional polymers with versatile functionalities via gemini monomers

    doi: 10.1126/sciadv.aaw9120

    Figure Lengend Snippet: Preparation and characterization of 2D net -poly(MA-11-2-11-MA). ( A ) Schematic illustration of the molecular structure of gemini monomer MA-11-2-11-MA and 2D net- poly(MA-11-2-11-MA). ( B ) AFM image and height analysis of the 2D net- poly(MA-11-2-11-MA) on the silicon substrate. Rq, root mean square roughness. ( C ) Simulation of the bilayer thickness with vertically arranged MA-11-2-11-MA monomers using Materials Studio. ( D ) TEM image of the freestanding film of 2D net -poly(MA-11-2-11-MA).

    Article Snippet: Adsorption isotherm of MA-11-2-11-MA on silicon The adsorption behavior of MA-11-2-11-MA at the solid-liquid interface play a critical role in the formation of the bilayer and the synthesis of net -poly(MA-11-2-11-MA).

    Techniques: Transmission Electron Microscopy

    DPD simulation of 2D net -poly(MA-11-2-11-MA). ( A ) Schematic illustration of the coarse-grained model of MA-11-2-11-MA. ( B ) Left: A representative snapshot of the MA-11-2-11-MA bilayer before polymerization. The conversion ratio of methacrylate groups is 0%. Right: Density profile of each component distributed in the vertical direction. Alkyl segments (black), ammonium groups (blue), methacrylate groups (red), and water (yellow). ( C ) Snapshots of MA-11-2-11-MA bilayers at increasing conversion ratios of methacrylate groups. ( D ) Left: A representative snapshot of the net -poly(MA-11-2-11-MA) bilayer after polymerization. The inset represents a top view of the poly(methacrylate) sublayer. The conversion ratio of the methacrylate groups is 98%. Right: Density profile of each component distributed in the vertical direction.

    Journal: Science Advances

    Article Title: Two-dimensional polymers with versatile functionalities via gemini monomers

    doi: 10.1126/sciadv.aaw9120

    Figure Lengend Snippet: DPD simulation of 2D net -poly(MA-11-2-11-MA). ( A ) Schematic illustration of the coarse-grained model of MA-11-2-11-MA. ( B ) Left: A representative snapshot of the MA-11-2-11-MA bilayer before polymerization. The conversion ratio of methacrylate groups is 0%. Right: Density profile of each component distributed in the vertical direction. Alkyl segments (black), ammonium groups (blue), methacrylate groups (red), and water (yellow). ( C ) Snapshots of MA-11-2-11-MA bilayers at increasing conversion ratios of methacrylate groups. ( D ) Left: A representative snapshot of the net -poly(MA-11-2-11-MA) bilayer after polymerization. The inset represents a top view of the poly(methacrylate) sublayer. The conversion ratio of the methacrylate groups is 98%. Right: Density profile of each component distributed in the vertical direction.

    Article Snippet: Adsorption isotherm of MA-11-2-11-MA on silicon The adsorption behavior of MA-11-2-11-MA at the solid-liquid interface play a critical role in the formation of the bilayer and the synthesis of net -poly(MA-11-2-11-MA).

    Techniques:

    NMR characterization of MA-11-2-11-MA assembly before and after polymerization. ( A ) Schematic illustration of the proton number in MA-11-2-11-MA. ( B ) 1 H NMR spectra of MA-11-2-11-MA assembly in D 2 O at 65°C (top) and the corresponding ROESY spectra by the selective irradiation of proton H-9 (bottom). ( C ) ROESY spectra of MA-11-2-11-MA assembly by the selective irradiation of H-9 at increased conversion ratios of methacrylate groups. The conversion ratios were estimated from the FT-IR spectra.

    Journal: Science Advances

    Article Title: Two-dimensional polymers with versatile functionalities via gemini monomers

    doi: 10.1126/sciadv.aaw9120

    Figure Lengend Snippet: NMR characterization of MA-11-2-11-MA assembly before and after polymerization. ( A ) Schematic illustration of the proton number in MA-11-2-11-MA. ( B ) 1 H NMR spectra of MA-11-2-11-MA assembly in D 2 O at 65°C (top) and the corresponding ROESY spectra by the selective irradiation of proton H-9 (bottom). ( C ) ROESY spectra of MA-11-2-11-MA assembly by the selective irradiation of H-9 at increased conversion ratios of methacrylate groups. The conversion ratios were estimated from the FT-IR spectra.

    Article Snippet: Adsorption isotherm of MA-11-2-11-MA on silicon The adsorption behavior of MA-11-2-11-MA at the solid-liquid interface play a critical role in the formation of the bilayer and the synthesis of net -poly(MA-11-2-11-MA).

    Techniques: Nuclear Magnetic Resonance, Irradiation

    Functionalization of 2D net -poly(MA-11-2-11-MA). ( A ) Chemical structures of the monomeric derivatives (MA-11-F; F, various functional groups) and copolymerization with MA-11-2-11-MA. ( B ) Fluorescence films of net- poly(MA-11-2-11-MA)- co -(MA-11-F) (F = RhB, Fls, or Pyr) in number and letter shapes (scale bar, 500 μm). ( C ) Fluorescence microscope images of net- poly(MA-11-2-11-MA)- co -(MA-11-C≡C)–coated silica wool after post-polymerization functionalization with RhB-EO 4 -N 3 , Fls-EO 4 -N 3 , and Pyr-EO 4 -N 3 via click reactions (scale bars, 200 μm).

    Journal: Science Advances

    Article Title: Two-dimensional polymers with versatile functionalities via gemini monomers

    doi: 10.1126/sciadv.aaw9120

    Figure Lengend Snippet: Functionalization of 2D net -poly(MA-11-2-11-MA). ( A ) Chemical structures of the monomeric derivatives (MA-11-F; F, various functional groups) and copolymerization with MA-11-2-11-MA. ( B ) Fluorescence films of net- poly(MA-11-2-11-MA)- co -(MA-11-F) (F = RhB, Fls, or Pyr) in number and letter shapes (scale bar, 500 μm). ( C ) Fluorescence microscope images of net- poly(MA-11-2-11-MA)- co -(MA-11-C≡C)–coated silica wool after post-polymerization functionalization with RhB-EO 4 -N 3 , Fls-EO 4 -N 3 , and Pyr-EO 4 -N 3 via click reactions (scale bars, 200 μm).

    Article Snippet: Adsorption isotherm of MA-11-2-11-MA on silicon The adsorption behavior of MA-11-2-11-MA at the solid-liquid interface play a critical role in the formation of the bilayer and the synthesis of net -poly(MA-11-2-11-MA).

    Techniques: Functional Assay, Fluorescence, Microscopy

    UNC93B1 association with STIM1 ER-luminal is required for STIM1 oligomerization. a Schematic representation of STIM1 full-length protein (WT) and STIM1 mutants (STIM1-ΔCt and STIM1-CAD) used in this study. b Fibroblasts expressing GFP-tagged STIM1- WT, −ΔCT and −CAD and UNC93B1 WT -FLAG-tagged plasmids were immunoprecipitated with GFP beads and immunoblotted with anti-GFP and anti-FLAG antibodies. One experiment representative out of two is shown. c STIM1 oligomerization was followed in HeLa cells in the presence of either Cherry-tagged WT or 3d-mutated UNC93B1 by measuring the increase in the FRET efficiency of YFP-tagged and CFP-tagged STIM1 upon stimulation with 1 μM TG over time. The V50 parameter is reported as the time-to-half–maximum FRET efficiency. Graph shows mean ± S.E.M. ( n = 3 experiments); * P

    Journal: Nature Communications

    Article Title: UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells

    doi: 10.1038/s41467-017-01601-5

    Figure Lengend Snippet: UNC93B1 association with STIM1 ER-luminal is required for STIM1 oligomerization. a Schematic representation of STIM1 full-length protein (WT) and STIM1 mutants (STIM1-ΔCt and STIM1-CAD) used in this study. b Fibroblasts expressing GFP-tagged STIM1- WT, −ΔCT and −CAD and UNC93B1 WT -FLAG-tagged plasmids were immunoprecipitated with GFP beads and immunoblotted with anti-GFP and anti-FLAG antibodies. One experiment representative out of two is shown. c STIM1 oligomerization was followed in HeLa cells in the presence of either Cherry-tagged WT or 3d-mutated UNC93B1 by measuring the increase in the FRET efficiency of YFP-tagged and CFP-tagged STIM1 upon stimulation with 1 μM TG over time. The V50 parameter is reported as the time-to-half–maximum FRET efficiency. Graph shows mean ± S.E.M. ( n = 3 experiments); * P

    Article Snippet: For immunoprecipitation of over-expressed proteins in fibroblasts, 4 mg of cell lysate was incubated o/n with protein G beads coated with anti-FLAG antibody or GFP–Trap_MA beads from Chromotek.

    Techniques: Expressing, Immunoprecipitation

    Active STIM1 restores antigen proteolysis and cross-presentation in 3d/3d DCs. a Detection of STIM1 active (STIM1-D76A) and UNC93B1 interaction using Duolink proximity ligation assay (PLA) with anti-GFP (STIM1-D76A) and anti-UNC93B1-specific antibodies in WT or 3d/3d DCs. Quantification of mean fluorescence using ImageJ software ( n = 12 cells; mean ± S.E.M.; ns for non-significant). One experiment out of three is shown. Bars = 10 μm. b Fibroblasts expressing GFP-tagged STIM1-D76A and WT or 3d-mutated UNC93B1-FLAG-tagged plasmids were immunoprecipitated with GFP beads (STIM1-D76A) and immunoblotted with anti-GFP and anti-FLAG antibodies. One experiment representative out of two is shown. c Fibroblasts were transiently co-transduced with STIM1-D76A-GFP and WT or 3d-mutated UNC93B1-Cherry-tagged plasmids. Representative TIRF and EPI images of STIM1 active and UNC93B1 (left panel) and quantification of percentage of GFP fluorescence intensity per cell area using ImageJ software ( n = 15 cells). One experiment out of two is shown. Bars = 10 μm. d DCs from WT and 3d/3d mice, transfected with STIM1-D76A-GFP or control GFP plasmids, were challenged with OVAb for 6 h before co-culture with CFSE-labeled OT-I T cells. T cell proliferation was monitored by flow cytometry 3 days later ( n = 3; mean ± S.E.M.; * P

    Journal: Nature Communications

    Article Title: UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells

    doi: 10.1038/s41467-017-01601-5

    Figure Lengend Snippet: Active STIM1 restores antigen proteolysis and cross-presentation in 3d/3d DCs. a Detection of STIM1 active (STIM1-D76A) and UNC93B1 interaction using Duolink proximity ligation assay (PLA) with anti-GFP (STIM1-D76A) and anti-UNC93B1-specific antibodies in WT or 3d/3d DCs. Quantification of mean fluorescence using ImageJ software ( n = 12 cells; mean ± S.E.M.; ns for non-significant). One experiment out of three is shown. Bars = 10 μm. b Fibroblasts expressing GFP-tagged STIM1-D76A and WT or 3d-mutated UNC93B1-FLAG-tagged plasmids were immunoprecipitated with GFP beads (STIM1-D76A) and immunoblotted with anti-GFP and anti-FLAG antibodies. One experiment representative out of two is shown. c Fibroblasts were transiently co-transduced with STIM1-D76A-GFP and WT or 3d-mutated UNC93B1-Cherry-tagged plasmids. Representative TIRF and EPI images of STIM1 active and UNC93B1 (left panel) and quantification of percentage of GFP fluorescence intensity per cell area using ImageJ software ( n = 15 cells). One experiment out of two is shown. Bars = 10 μm. d DCs from WT and 3d/3d mice, transfected with STIM1-D76A-GFP or control GFP plasmids, were challenged with OVAb for 6 h before co-culture with CFSE-labeled OT-I T cells. T cell proliferation was monitored by flow cytometry 3 days later ( n = 3; mean ± S.E.M.; * P

    Article Snippet: For immunoprecipitation of over-expressed proteins in fibroblasts, 4 mg of cell lysate was incubated o/n with protein G beads coated with anti-FLAG antibody or GFP–Trap_MA beads from Chromotek.

    Techniques: Proximity Ligation Assay, Fluorescence, Software, Expressing, Immunoprecipitation, Transduction, Mouse Assay, Transfection, Co-Culture Assay, Labeling, Flow Cytometry, Cytometry

    Reduced Unc93b1 3 d -STIM1 association compromises STIM1 function. a Fibroblasts expressing STIM1-WT-GFP-tagged and UNC93B1 WT or UNC93B1 3d -FLAG-tagged, plasmids were lysed and STIM1 was immunoprecipitated with GFP beads and immunoblotted with anti-GFP and anti-FLAG antibodies. b In WT and 3d/3d DCs transfected with STIM1-WT-GFP-plasmid, STIM1–UNC93B1 interaction was detected using Duolink proximity ligation assay with anti-GFP and anti-UNC93B1-specific antibodies. Cells are seen in brightfield (top panel). PLA signals are shown in red and nuclei in blue (bottom panel) and quantified with Image J ( n = 11 cells; * P

    Journal: Nature Communications

    Article Title: UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells

    doi: 10.1038/s41467-017-01601-5

    Figure Lengend Snippet: Reduced Unc93b1 3 d -STIM1 association compromises STIM1 function. a Fibroblasts expressing STIM1-WT-GFP-tagged and UNC93B1 WT or UNC93B1 3d -FLAG-tagged, plasmids were lysed and STIM1 was immunoprecipitated with GFP beads and immunoblotted with anti-GFP and anti-FLAG antibodies. b In WT and 3d/3d DCs transfected with STIM1-WT-GFP-plasmid, STIM1–UNC93B1 interaction was detected using Duolink proximity ligation assay with anti-GFP and anti-UNC93B1-specific antibodies. Cells are seen in brightfield (top panel). PLA signals are shown in red and nuclei in blue (bottom panel) and quantified with Image J ( n = 11 cells; * P

    Article Snippet: For immunoprecipitation of over-expressed proteins in fibroblasts, 4 mg of cell lysate was incubated o/n with protein G beads coated with anti-FLAG antibody or GFP–Trap_MA beads from Chromotek.

    Techniques: Expressing, Immunoprecipitation, Transfection, Plasmid Preparation, Proximity Ligation Assay