zr plasmid miniprep kit  (Zymo Research)


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    Name:
    ZymoPURE Plasmid Miniprep Kit
    Description:
    The ZymoPURE Plasmid Miniprep Kit features a spin column based method for the purification of up to 100 µg of ultra pure endotoxin free plasmid DNA in less than 15 minutes The unique spin column design also provides zero buffer retention and a low elution volume ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA up to 3 µg µl In addition the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins salt protein and RNA The result is plasmid DNA suitable for transfection restriction endonuclease digestion bacterial transformation PCR amplification DNA sequencing and other sensitive downstream applications As an added convenience the ZymoPURE Plasmid Miniprep Kit contains colored buffers that permit error free visualization and identification of complete bacterial cell lysis and neutralization
    Catalog Number:
    d4208t
    Product Aliases:
    ZymoPURE II Plasmid Miniprep Kit
    Price:
    None
    Applications:
    Plasmid DNA Purification
    Size:
    10 units
    Category:
    Life Science Reagents and Media
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    Structured Review

    Zymo Research zr plasmid miniprep kit
    ZymoPURE Plasmid Miniprep Kit
    The ZymoPURE Plasmid Miniprep Kit features a spin column based method for the purification of up to 100 µg of ultra pure endotoxin free plasmid DNA in less than 15 minutes The unique spin column design also provides zero buffer retention and a low elution volume ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA up to 3 µg µl In addition the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins salt protein and RNA The result is plasmid DNA suitable for transfection restriction endonuclease digestion bacterial transformation PCR amplification DNA sequencing and other sensitive downstream applications As an added convenience the ZymoPURE Plasmid Miniprep Kit contains colored buffers that permit error free visualization and identification of complete bacterial cell lysis and neutralization
    https://www.bioz.com/result/zr plasmid miniprep kit/product/Zymo Research
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    zr plasmid miniprep kit - by Bioz Stars, 2020-07
    95/100 stars

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    Related Articles

    Sequencing:

    Article Title: Contribution of non-coding mutations to RPGRIP1-mediated inherited retinal degeneration
    Article Snippet: .. Plasmid DNA from single colonies was extracted with miniprep kits (ZymoPURE, Zymo Research) and analyzed by restriction enzyme digestion with BsrGI (NE Biolabs, Ipswich, MA) and Sanger sequencing. .. Essential splice-site mutations were introduced by site-directed mutagenesis (QuickChange II Site Directed mutagenesis kit, Agilent Technologies) and verified by Sanger sequencing.

    Isolation:

    Article Title: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
    Article Snippet: .. Plasmid DNA was isolated from kanamycin resistant, white colonies via ZymoPURE Plasmid Miniprep Kit (Zymo Research, Irvine, CA). .. Modifications made to plasmid DNA were evaluated after DNA sequencing (GeneWiz, South Plainfield, NJ) and disruption patterns were assessed using SnapGene software.

    Article Title: Infectious vaccine-derived rubella viruses emerge, persist, and evolve in cutaneous granulomas of children with primary immunodeficiencies
    Article Snippet: .. Plasmid DNA was isolated using a ZymoPure Plasmid Miniprep Kit (Zymo Research) and the plasmid inserts were sequenced with the M13 forward and reverse primers and the RV primers [ ]. ..

    Plasmid Preparation:

    Article Title: Contribution of non-coding mutations to RPGRIP1-mediated inherited retinal degeneration
    Article Snippet: .. Plasmid DNA from single colonies was extracted with miniprep kits (ZymoPURE, Zymo Research) and analyzed by restriction enzyme digestion with BsrGI (NE Biolabs, Ipswich, MA) and Sanger sequencing. .. Essential splice-site mutations were introduced by site-directed mutagenesis (QuickChange II Site Directed mutagenesis kit, Agilent Technologies) and verified by Sanger sequencing.

    Article Title: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
    Article Snippet: .. Plasmid DNA was isolated from kanamycin resistant, white colonies via ZymoPURE Plasmid Miniprep Kit (Zymo Research, Irvine, CA). .. Modifications made to plasmid DNA were evaluated after DNA sequencing (GeneWiz, South Plainfield, NJ) and disruption patterns were assessed using SnapGene software.

    Article Title: Infectious vaccine-derived rubella viruses emerge, persist, and evolve in cutaneous granulomas of children with primary immunodeficiencies
    Article Snippet: .. Plasmid DNA was isolated using a ZymoPure Plasmid Miniprep Kit (Zymo Research) and the plasmid inserts were sequenced with the M13 forward and reverse primers and the RV primers [ ]. ..

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  • 99
    Zymo Research zr duet rna dna miniprep kit
    GC content and dinucleotide composition are associated with regulatory activity in U87 cells and hNPCs. ( A, D ) Distributions of expression values for all cis- regulatory elements in U87 cells (A) and hNPCs (D). Expression values are averaged across the three biological replicates and plotted as log 2 <t>(RNA</t> read <t>count/DNA</t> read count). ( B, E ) Box plots displaying GC content for high and low activities cis- regulatory elements in U87 cells (B) and hNPCs (E). GC content is significantly different between high- and low-activity elements in both cell types ( P
    Zr Duet Rna Dna Miniprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zr duet rna dna miniprep kit/product/Zymo Research
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zr duet rna dna miniprep kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    95
    Zymo Research zr plasmid miniprep classic kit
    GC content and dinucleotide composition are associated with regulatory activity in U87 cells and hNPCs. ( A, D ) Distributions of expression values for all cis- regulatory elements in U87 cells (A) and hNPCs (D). Expression values are averaged across the three biological replicates and plotted as log 2 <t>(RNA</t> read <t>count/DNA</t> read count). ( B, E ) Box plots displaying GC content for high and low activities cis- regulatory elements in U87 cells (B) and hNPCs (E). GC content is significantly different between high- and low-activity elements in both cell types ( P
    Zr Plasmid Miniprep Classic Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zr plasmid miniprep classic kit/product/Zymo Research
    Average 95 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    zr plasmid miniprep classic kit - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    Image Search Results


    GC content and dinucleotide composition are associated with regulatory activity in U87 cells and hNPCs. ( A, D ) Distributions of expression values for all cis- regulatory elements in U87 cells (A) and hNPCs (D). Expression values are averaged across the three biological replicates and plotted as log 2 (RNA read count/DNA read count). ( B, E ) Box plots displaying GC content for high and low activities cis- regulatory elements in U87 cells (B) and hNPCs (E). GC content is significantly different between high- and low-activity elements in both cell types ( P

    Journal: Nucleic Acids Research

    Article Title: A genome-integrated massively parallel reporter assay reveals DNA sequence determinants of cis-regulatory activity in neural cells

    doi: 10.1093/nar/gkw942

    Figure Lengend Snippet: GC content and dinucleotide composition are associated with regulatory activity in U87 cells and hNPCs. ( A, D ) Distributions of expression values for all cis- regulatory elements in U87 cells (A) and hNPCs (D). Expression values are averaged across the three biological replicates and plotted as log 2 (RNA read count/DNA read count). ( B, E ) Box plots displaying GC content for high and low activities cis- regulatory elements in U87 cells (B) and hNPCs (E). GC content is significantly different between high- and low-activity elements in both cell types ( P

    Article Snippet: Measuring regulatory activity with RNA-seq Total RNA was extracted from U87 cells and hNPCs 96 h after transduction using the ZR-Duet RNA/DNA MiniPrep kit (Zymo Research).

    Techniques: Activity Assay, Expressing

    Quantitative LV-MPRA expression measurements are reproducible. ( A ) Putative cis -regulatory elements cloned upstream of an Hspa1b minimal promoter drive expression of dsRed containing DNA sequence barcodes in the 3΄ UTR. 2600 unique reporter gene constructs were cloned in parallel and the complex lentiviral plasmid library was used to produce lentivirus for transduction. U87 cells and human neural progenitor cells were transduced in triplicate and RNA/DNA were extracted after 96 h in culture. ( B ) Scatter plot showing expression measurements for cis -regulatory elements from two biological replicates in U87 cells. Expression in each replicate is plotted as log 2 (RNA read count/DNA read count). Correlation between expression values of regulatory elements with measurements for all five barcodes is 0.70. ( C ) Scatter plot from two biological replicates in human neural progenitor cells. Expression in each replicate is plotted as log 2 (RNA read count/DNA read count). Correlation between expression values of regulatory elements with measurements for all five barcodes is 0.63.

    Journal: Nucleic Acids Research

    Article Title: A genome-integrated massively parallel reporter assay reveals DNA sequence determinants of cis-regulatory activity in neural cells

    doi: 10.1093/nar/gkw942

    Figure Lengend Snippet: Quantitative LV-MPRA expression measurements are reproducible. ( A ) Putative cis -regulatory elements cloned upstream of an Hspa1b minimal promoter drive expression of dsRed containing DNA sequence barcodes in the 3΄ UTR. 2600 unique reporter gene constructs were cloned in parallel and the complex lentiviral plasmid library was used to produce lentivirus for transduction. U87 cells and human neural progenitor cells were transduced in triplicate and RNA/DNA were extracted after 96 h in culture. ( B ) Scatter plot showing expression measurements for cis -regulatory elements from two biological replicates in U87 cells. Expression in each replicate is plotted as log 2 (RNA read count/DNA read count). Correlation between expression values of regulatory elements with measurements for all five barcodes is 0.70. ( C ) Scatter plot from two biological replicates in human neural progenitor cells. Expression in each replicate is plotted as log 2 (RNA read count/DNA read count). Correlation between expression values of regulatory elements with measurements for all five barcodes is 0.63.

    Article Snippet: Measuring regulatory activity with RNA-seq Total RNA was extracted from U87 cells and hNPCs 96 h after transduction using the ZR-Duet RNA/DNA MiniPrep kit (Zymo Research).

    Techniques: Expressing, Clone Assay, Sequencing, Construct, Plasmid Preparation, Transduction

    Different DNA motifs are enriched in high expressing cis- regulatory elements in U87 cells and hNPCs. ( A ) Scatter plot showing average expression measurements for regulatory elements in U87 cells and hNPCs. Expression in each cell type is plotted as log 2 (RNA read count/DNA read count). Activity in the two cell types is significantly negatively correlated ( R = −0.326, P = 5.02e−53, Pearson's product-moment correlation). ( B ) AME analysis identified motifs enriched in regulatory elements with high activity in U87 cells relative to elements with high activity in hNPCs. ( C ) Motif enrichment in hNPC High Elements relative to U87 High Elements. Motifs enriched in U87 cells ( P

    Journal: Nucleic Acids Research

    Article Title: A genome-integrated massively parallel reporter assay reveals DNA sequence determinants of cis-regulatory activity in neural cells

    doi: 10.1093/nar/gkw942

    Figure Lengend Snippet: Different DNA motifs are enriched in high expressing cis- regulatory elements in U87 cells and hNPCs. ( A ) Scatter plot showing average expression measurements for regulatory elements in U87 cells and hNPCs. Expression in each cell type is plotted as log 2 (RNA read count/DNA read count). Activity in the two cell types is significantly negatively correlated ( R = −0.326, P = 5.02e−53, Pearson's product-moment correlation). ( B ) AME analysis identified motifs enriched in regulatory elements with high activity in U87 cells relative to elements with high activity in hNPCs. ( C ) Motif enrichment in hNPC High Elements relative to U87 High Elements. Motifs enriched in U87 cells ( P

    Article Snippet: Measuring regulatory activity with RNA-seq Total RNA was extracted from U87 cells and hNPCs 96 h after transduction using the ZR-Duet RNA/DNA MiniPrep kit (Zymo Research).

    Techniques: Expressing, Activity Assay