zr duet rna dna miniprep kit  (Zymo Research)


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    Name:
    Quick DNA RNA Miniprep
    Description:
    The Quick DNA RNA Miniprep Kit provides a quick method for the isolation of high quality genomic DNA and total RNA from small amounts of cells and tissue The kit isolates both genomic DNA and a broad range of RNA species without the use of phenol Small RNAs e g tRNAs microRNAs can be recovered following a simple adjustment within the RNA isolation protocol no extra steps are required Both DNA and RNA from up to 5x106 cells can be eluted into volumes as little as 25 µl in less than 15 minutes
    Catalog Number:
    d7001
    Product Aliases:
    ZR-Duet DNA/RNA Miniprep
    Price:
    None
    Applications:
    DNA/RNA Co-Purification
    Size:
    50 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research zr duet rna dna miniprep kit
    Quick DNA RNA Miniprep
    The Quick DNA RNA Miniprep Kit provides a quick method for the isolation of high quality genomic DNA and total RNA from small amounts of cells and tissue The kit isolates both genomic DNA and a broad range of RNA species without the use of phenol Small RNAs e g tRNAs microRNAs can be recovered following a simple adjustment within the RNA isolation protocol no extra steps are required Both DNA and RNA from up to 5x106 cells can be eluted into volumes as little as 25 µl in less than 15 minutes
    https://www.bioz.com/result/zr duet rna dna miniprep kit/product/Zymo Research
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zr duet rna dna miniprep kit - by Bioz Stars, 2020-08
    99/100 stars

    Related Products / Commonly Used Together

    u87 cells
    hnpcs
    rna-seq total rna

    Images

    1) Product Images from "A genome-integrated massively parallel reporter assay reveals DNA sequence determinants of cis-regulatory activity in neural cells"

    Article Title: A genome-integrated massively parallel reporter assay reveals DNA sequence determinants of cis-regulatory activity in neural cells

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkw942

    GC content and dinucleotide composition are associated with regulatory activity in U87 cells and hNPCs. ( A, D ) Distributions of expression values for all cis- regulatory elements in U87 cells (A) and hNPCs (D). Expression values are averaged across the three biological replicates and plotted as log 2 (RNA read count/DNA read count). ( B, E ) Box plots displaying GC content for high and low activities cis- regulatory elements in U87 cells (B) and hNPCs (E). GC content is significantly different between high- and low-activity elements in both cell types ( P
    Figure Legend Snippet: GC content and dinucleotide composition are associated with regulatory activity in U87 cells and hNPCs. ( A, D ) Distributions of expression values for all cis- regulatory elements in U87 cells (A) and hNPCs (D). Expression values are averaged across the three biological replicates and plotted as log 2 (RNA read count/DNA read count). ( B, E ) Box plots displaying GC content for high and low activities cis- regulatory elements in U87 cells (B) and hNPCs (E). GC content is significantly different between high- and low-activity elements in both cell types ( P

    Techniques Used: Activity Assay, Expressing

    Quantitative LV-MPRA expression measurements are reproducible. ( A ) Putative cis -regulatory elements cloned upstream of an Hspa1b minimal promoter drive expression of dsRed containing DNA sequence barcodes in the 3΄ UTR. 2600 unique reporter gene constructs were cloned in parallel and the complex lentiviral plasmid library was used to produce lentivirus for transduction. U87 cells and human neural progenitor cells were transduced in triplicate and RNA/DNA were extracted after 96 h in culture. ( B ) Scatter plot showing expression measurements for cis -regulatory elements from two biological replicates in U87 cells. Expression in each replicate is plotted as log 2 (RNA read count/DNA read count). Correlation between expression values of regulatory elements with measurements for all five barcodes is 0.70. ( C ) Scatter plot from two biological replicates in human neural progenitor cells. Expression in each replicate is plotted as log 2 (RNA read count/DNA read count). Correlation between expression values of regulatory elements with measurements for all five barcodes is 0.63.
    Figure Legend Snippet: Quantitative LV-MPRA expression measurements are reproducible. ( A ) Putative cis -regulatory elements cloned upstream of an Hspa1b minimal promoter drive expression of dsRed containing DNA sequence barcodes in the 3΄ UTR. 2600 unique reporter gene constructs were cloned in parallel and the complex lentiviral plasmid library was used to produce lentivirus for transduction. U87 cells and human neural progenitor cells were transduced in triplicate and RNA/DNA were extracted after 96 h in culture. ( B ) Scatter plot showing expression measurements for cis -regulatory elements from two biological replicates in U87 cells. Expression in each replicate is plotted as log 2 (RNA read count/DNA read count). Correlation between expression values of regulatory elements with measurements for all five barcodes is 0.70. ( C ) Scatter plot from two biological replicates in human neural progenitor cells. Expression in each replicate is plotted as log 2 (RNA read count/DNA read count). Correlation between expression values of regulatory elements with measurements for all five barcodes is 0.63.

    Techniques Used: Expressing, Clone Assay, Sequencing, Construct, Plasmid Preparation, Transduction

    Different DNA motifs are enriched in high expressing cis- regulatory elements in U87 cells and hNPCs. ( A ) Scatter plot showing average expression measurements for regulatory elements in U87 cells and hNPCs. Expression in each cell type is plotted as log 2 (RNA read count/DNA read count). Activity in the two cell types is significantly negatively correlated ( R = −0.326, P = 5.02e−53, Pearson's product-moment correlation). ( B ) AME analysis identified motifs enriched in regulatory elements with high activity in U87 cells relative to elements with high activity in hNPCs. ( C ) Motif enrichment in hNPC High Elements relative to U87 High Elements. Motifs enriched in U87 cells ( P
    Figure Legend Snippet: Different DNA motifs are enriched in high expressing cis- regulatory elements in U87 cells and hNPCs. ( A ) Scatter plot showing average expression measurements for regulatory elements in U87 cells and hNPCs. Expression in each cell type is plotted as log 2 (RNA read count/DNA read count). Activity in the two cell types is significantly negatively correlated ( R = −0.326, P = 5.02e−53, Pearson's product-moment correlation). ( B ) AME analysis identified motifs enriched in regulatory elements with high activity in U87 cells relative to elements with high activity in hNPCs. ( C ) Motif enrichment in hNPC High Elements relative to U87 High Elements. Motifs enriched in U87 cells ( P

    Techniques Used: Expressing, Activity Assay

    Related Articles

    Mass Spectrometry:

    Article Title: Epigenetic reprogramming enables the primordial germ cell-to-gonocyte transition
    Article Snippet: .. Mass spectrometry Genomic DNA from between 100 and 2,000 FACS-sorted PGCs was extracted using ZR-Duet DNA/RNA Miniprep kit (Zymo Reasearch) following manufacturer instructions and eluted in LC/MS grade water. .. DNA was digested to nucleosides using a digestion enzyme mix provided by NEB.

    Isolation:

    Article Title: Epigenetic reprogramming enables the primordial germ cell-to-gonocyte transition
    Article Snippet: .. For study of mESCs, total RNA was isolated using ZR-Duet DNA-RNA MiniPrep kit (Zymo). cDNA synthesis and library prep was performed starting with 500 ng total RNA following manufacturer’s instructions using the NEBNext Ultra Library Prep Kit (NEB) and the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). .. All libraries were purified by AMPure XP beads (Beckman-Coulter) and sequenced on the Illumina HiSeq 2500 instrument.

    Article Title: An epigenetic mechanism for cavefish eye degeneration
    Article Snippet: .. RNA isolation and sequencing Total cellular RNA and DNA was isolated from the harvested eyes using ZR Duet DNA/RNA miniprep kit (Zymo Research). .. 300-900ng poly-A enriched RNA was converted to indexed sequencing libraries using the TruSeq Standard mRNA library prep kit (Illumina).

    Article Title: Ancestral perinatal obesogen exposure results in a transgenerational thrifty phenotype in mice
    Article Snippet: .. Genomic DNA (gDNA) and RNA were isolated from male gWAT using ZR-Duet™ DNA/RNA MiniPrep kit (Zymo Research) of animals sacrificed at 33 weeks of age (after the diet challenge). .. Nucleic acid samples from 4 randomly selected mice from control or TBT groups were submitted for DNA methylome and transcriptome analyses.

    Real-time Polymerase Chain Reaction:

    Article Title: Follicular Helper T Cells Are Major Human Immunodeficiency Virus-2 Reservoirs and Support Productive Infection
    Article Snippet: .. Quantification of Cell-Associated Human Immunodeficiency Virus Deoxyribonucleic Acid and gag Messenger Ribonucleic Acid by Real-Time Polymerase Chain Reaction Total DNA and RNA were extracted from cell pellets of in vitro-infected Tfh and sort-purified blood CD4 T-cell subsets, using AllPrep DNA/RNA Micro kit (QIAGEN) and ZR-Duet DNA/RNA MiniPrep kit (Zymo Research), respectively. .. For gag messenger RNA (mRNA) quantification, 80 ng of total RNA was used to synthesize complementary DNA using oligo(dT)20 and SuperScript III RT (Invitrogen).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Epigenetic reprogramming enables the primordial germ cell-to-gonocyte transition
    Article Snippet: .. Mass spectrometry Genomic DNA from between 100 and 2,000 FACS-sorted PGCs was extracted using ZR-Duet DNA/RNA Miniprep kit (Zymo Reasearch) following manufacturer instructions and eluted in LC/MS grade water. .. DNA was digested to nucleosides using a digestion enzyme mix provided by NEB.

    Sequencing:

    Article Title: An epigenetic mechanism for cavefish eye degeneration
    Article Snippet: .. RNA isolation and sequencing Total cellular RNA and DNA was isolated from the harvested eyes using ZR Duet DNA/RNA miniprep kit (Zymo Research). .. 300-900ng poly-A enriched RNA was converted to indexed sequencing libraries using the TruSeq Standard mRNA library prep kit (Illumina).

    FACS:

    Article Title: Epigenetic reprogramming enables the primordial germ cell-to-gonocyte transition
    Article Snippet: .. Mass spectrometry Genomic DNA from between 100 and 2,000 FACS-sorted PGCs was extracted using ZR-Duet DNA/RNA Miniprep kit (Zymo Reasearch) following manufacturer instructions and eluted in LC/MS grade water. .. DNA was digested to nucleosides using a digestion enzyme mix provided by NEB.

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  • 96
    Zymo Research parallel dna rna extraction
    Microbial alignments detected in the lungs of immunocompromised children. Red dots represent potentially pathogenic microbes that are both abundant (≥10 rpm for bacteria or ≥1 rpm for fungi or viruses) and identified at levels greater than most other samples in the cohort (Z-score ≥2). Hollow red dots indicating Bocavirus and Pneumocystis are used to indicate organisms observed only once in this cohort. Blue dots represent all other potentially pathogenic microbes; light blue dots represent typically nonpathogenic microbes. Subplots show (A ) all bacteria, (B ) fungi, (C ) <t>RNA</t> viruses, and (D ) <t>DNA</t> viruses identified across all samples in the cohort. For the purpose of the Z-score calculation, the value of log 10 -transformed reads for undetected microbes was assumed to equal –2, just below the lower limit of detection for our sequencing depth (log 10 [0.01rpm] = –2). Abbreviation: seq, sequencing.
    Parallel Dna Rna Extraction, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/parallel dna rna extraction/product/Zymo Research
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    parallel dna rna extraction - by Bioz Stars, 2020-08
    96/100 stars
      Buy from Supplier

    99
    Zymo Research zr duet dna rna miniprep kit
    Identification of differentially abundant NOGs at the level of <t>DNA</t> and transcription. ( a ) Overlap of NOGs detected with ⩾1 read in ⩾1 sample in metagenomic and metatranscriptomic analyses. ( b ) Distributions of reads per million (RPM) for NOGs detected in DNA-seq, <t>RNA-seq</t> or both data sets (average across 16 samples). *Wilcoxon rank-sum test P
    Zr Duet Dna Rna Miniprep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zr duet dna rna miniprep kit/product/Zymo Research
    Average 99 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    zr duet dna rna miniprep kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Microbial alignments detected in the lungs of immunocompromised children. Red dots represent potentially pathogenic microbes that are both abundant (≥10 rpm for bacteria or ≥1 rpm for fungi or viruses) and identified at levels greater than most other samples in the cohort (Z-score ≥2). Hollow red dots indicating Bocavirus and Pneumocystis are used to indicate organisms observed only once in this cohort. Blue dots represent all other potentially pathogenic microbes; light blue dots represent typically nonpathogenic microbes. Subplots show (A ) all bacteria, (B ) fungi, (C ) RNA viruses, and (D ) DNA viruses identified across all samples in the cohort. For the purpose of the Z-score calculation, the value of log 10 -transformed reads for undetected microbes was assumed to equal –2, just below the lower limit of detection for our sequencing depth (log 10 [0.01rpm] = –2). Abbreviation: seq, sequencing.

    Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

    Article Title: Pulmonary Metagenomic Sequencing Suggests Missed Infections in Immunocompromised Children

    doi: 10.1093/cid/ciy802

    Figure Lengend Snippet: Microbial alignments detected in the lungs of immunocompromised children. Red dots represent potentially pathogenic microbes that are both abundant (≥10 rpm for bacteria or ≥1 rpm for fungi or viruses) and identified at levels greater than most other samples in the cohort (Z-score ≥2). Hollow red dots indicating Bocavirus and Pneumocystis are used to indicate organisms observed only once in this cohort. Blue dots represent all other potentially pathogenic microbes; light blue dots represent typically nonpathogenic microbes. Subplots show (A ) all bacteria, (B ) fungi, (C ) RNA viruses, and (D ) DNA viruses identified across all samples in the cohort. For the purpose of the Z-score calculation, the value of log 10 -transformed reads for undetected microbes was assumed to equal –2, just below the lower limit of detection for our sequencing depth (log 10 [0.01rpm] = –2). Abbreviation: seq, sequencing.

    Article Snippet: Subsequently, all samples underwent 10 minutes of centrifugation at 4°C, and the supernatant was used for parallel DNA/RNA extraction (Zymo ZR-Duet DNA/RNA MiniPrep Kit).

    Techniques: Transformation Assay, Sequencing

    Identification of differentially abundant NOGs at the level of DNA and transcription. ( a ) Overlap of NOGs detected with ⩾1 read in ⩾1 sample in metagenomic and metatranscriptomic analyses. ( b ) Distributions of reads per million (RPM) for NOGs detected in DNA-seq, RNA-seq or both data sets (average across 16 samples). *Wilcoxon rank-sum test P

    Journal: The ISME Journal

    Article Title: Defining the microbial transcriptional response to colitis through integrated host and microbiome profiling

    doi: 10.1038/ismej.2016.40

    Figure Lengend Snippet: Identification of differentially abundant NOGs at the level of DNA and transcription. ( a ) Overlap of NOGs detected with ⩾1 read in ⩾1 sample in metagenomic and metatranscriptomic analyses. ( b ) Distributions of reads per million (RPM) for NOGs detected in DNA-seq, RNA-seq or both data sets (average across 16 samples). *Wilcoxon rank-sum test P

    Article Snippet: Samples were centrifuged at 8000 g for 5 min and the supernatant was diluted with three volumes of lysing buffer (ZR-Duet DNA/RNA Miniprep kit, Zymo Research).

    Techniques: DNA Sequencing, RNA Sequencing Assay

    Differentially abundant genera in colitis versus steady state. ( a ) Overlap of genera detected with ⩾1 read in ⩾1 sample in metagenomic and metatranscriptomic analyses. ( b ) Distributions of reads per million (RPM) for genera detected in DNA-seq, RNA-seq or both data sets (average across 16 samples). *Wilcoxon rank-sum test P

    Journal: The ISME Journal

    Article Title: Defining the microbial transcriptional response to colitis through integrated host and microbiome profiling

    doi: 10.1038/ismej.2016.40

    Figure Lengend Snippet: Differentially abundant genera in colitis versus steady state. ( a ) Overlap of genera detected with ⩾1 read in ⩾1 sample in metagenomic and metatranscriptomic analyses. ( b ) Distributions of reads per million (RPM) for genera detected in DNA-seq, RNA-seq or both data sets (average across 16 samples). *Wilcoxon rank-sum test P

    Article Snippet: Samples were centrifuged at 8000 g for 5 min and the supernatant was diluted with three volumes of lysing buffer (ZR-Duet DNA/RNA Miniprep kit, Zymo Research).

    Techniques: DNA Sequencing, RNA Sequencing Assay

    Ancestral TBT exposure leads to altered DNA methylation and expression of the leptin gene. a Upper panel represents isoDMB #1626 (black bar), and its overlap with regions with different GC content. DMRs punctuating this hypomethylated isoDMB are represented with black vertical bars and numbered (1–2). Overexpressed genes within the isoDMB (Lep, leptin) are represented in green and genes whose expression does not change between DMSO and TBT are represented in gray. b Bottom panels show the variation for the mean ( n = 4) of MBD-seq read coverage for TBT and DMSO samples within 3000 bp regions with the 100 bp DMRs indicated with an asterisk. c RPKMs from RNA-seq analysis of leptin mRNA expression ( n = 4). D, DMSO; T, TBT. Statistical significance was determined using R (version 3.3), and Bioconductor (version 3.3) package edgeR (version 3.14) 54 . Data are presented as mean ± s.e.m. *p

    Journal: Nature Communications

    Article Title: Ancestral perinatal obesogen exposure results in a transgenerational thrifty phenotype in mice

    doi: 10.1038/s41467-017-01944-z

    Figure Lengend Snippet: Ancestral TBT exposure leads to altered DNA methylation and expression of the leptin gene. a Upper panel represents isoDMB #1626 (black bar), and its overlap with regions with different GC content. DMRs punctuating this hypomethylated isoDMB are represented with black vertical bars and numbered (1–2). Overexpressed genes within the isoDMB (Lep, leptin) are represented in green and genes whose expression does not change between DMSO and TBT are represented in gray. b Bottom panels show the variation for the mean ( n = 4) of MBD-seq read coverage for TBT and DMSO samples within 3000 bp regions with the 100 bp DMRs indicated with an asterisk. c RPKMs from RNA-seq analysis of leptin mRNA expression ( n = 4). D, DMSO; T, TBT. Statistical significance was determined using R (version 3.3), and Bioconductor (version 3.3) package edgeR (version 3.14) 54 . Data are presented as mean ± s.e.m. *p

    Article Snippet: Genomic DNA (gDNA) and RNA were isolated from male gWAT using ZR-Duet™ DNA/RNA MiniPrep kit (Zymo Research) of animals sacrificed at 33 weeks of age (after the diet challenge).

    Techniques: DNA Methylation Assay, Expressing, RNA Sequencing Assay

    Promoter DNA methylation clustering analysis during germline reprogramming. a) The combined promoter 5mC/5hmC levels (WGBS, right), promoter 5hmC levels (AbaSeq, centre), or gene expression levels (RNA-Seq, right) in consecutive stages of PGC development for all genes grouped by K-means clustering of the combined 5mC/5hmC dynamics at their promoter regions. b-c) section.

    Journal: Nature

    Article Title: Epigenetic reprogramming enables the primordial germ cell-to-gonocyte transition

    doi: 10.1038/nature25964

    Figure Lengend Snippet: Promoter DNA methylation clustering analysis during germline reprogramming. a) The combined promoter 5mC/5hmC levels (WGBS, right), promoter 5hmC levels (AbaSeq, centre), or gene expression levels (RNA-Seq, right) in consecutive stages of PGC development for all genes grouped by K-means clustering of the combined 5mC/5hmC dynamics at their promoter regions. b-c) section.

    Article Snippet: For study of mESCs, total RNA was isolated using ZR-Duet DNA-RNA MiniPrep kit (Zymo). cDNA synthesis and library prep was performed starting with 500 ng total RNA following manufacturer’s instructions using the NEBNext Ultra Library Prep Kit (NEB) and the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB).

    Techniques: DNA Methylation Assay, Expressing, RNA Sequencing Assay, Pyrolysis Gas Chromatography

    DNA modification and expression dynamics in wild type and Tet1 -KO PGCs at retrotransposons normally activated concurrent with epigenetic reprogramming. a-b) Combined 5mC/5hmC dynamics in wild type PGCs (%; WGBS; far left), relative 5hmC dynamics (AbaSeq read counts normalised to E10.5; centre left) in wild type PGCs, the expression dynamics in either wild type or Tet1 -KO PGCs (transcripts per million (TPM); RNA-Seq; centre right), and combined 5mC/5hmC dynamics in wild type and Tet1 -KO PGCs (%; RRBS; far right) for representative repetitive elements significantly up-regulated (adj. p-value

    Journal: Nature

    Article Title: Epigenetic reprogramming enables the primordial germ cell-to-gonocyte transition

    doi: 10.1038/nature25964

    Figure Lengend Snippet: DNA modification and expression dynamics in wild type and Tet1 -KO PGCs at retrotransposons normally activated concurrent with epigenetic reprogramming. a-b) Combined 5mC/5hmC dynamics in wild type PGCs (%; WGBS; far left), relative 5hmC dynamics (AbaSeq read counts normalised to E10.5; centre left) in wild type PGCs, the expression dynamics in either wild type or Tet1 -KO PGCs (transcripts per million (TPM); RNA-Seq; centre right), and combined 5mC/5hmC dynamics in wild type and Tet1 -KO PGCs (%; RRBS; far right) for representative repetitive elements significantly up-regulated (adj. p-value

    Article Snippet: For study of mESCs, total RNA was isolated using ZR-Duet DNA-RNA MiniPrep kit (Zymo). cDNA synthesis and library prep was performed starting with 500 ng total RNA following manufacturer’s instructions using the NEBNext Ultra Library Prep Kit (NEB) and the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB).

    Techniques: Modification, Expressing, RNA Sequencing Assay