zorbax eclipse xdb c18 column  (Agilent technologies)

 
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    Name:
    Zorbax
    Description:
    The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed phase HPLC ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution From ZORBAX Eclipse Plus C18 to sub 2 micron UHPLC columms a wide selection of stationary phases and particle sizes allows you to fine tune your selectivity to match your application
    Catalog Number:
    5188-5328
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    Products Small Molecule Columns Reversed Phase Hplc Columns Zorbax
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    Structured Review

    Agilent technologies zorbax eclipse xdb c18 column
    HPLC-QTOF-MS-derived base peak chromatogram of the HLM incubation supernatant featuring the parent compound vitetrifolin D (VD) and its phase I metabolites M1–M22. Experimental conditions HPLC: column: <t>Zorbax</t> Eclipse <t>XDB-C18,</t> 3 × 100 mm, 3.5 µm; mobile phase: A: H 2 O, B: acetonitrile, gradient: 0 min: 80% A, 5 min: 80% A, 8 min: 65% A, 35 min: 2% A; temp: 25 °C; flow rate 0.3 mL/min; injection volume:10 µL. HR-MS: ESI, positive mode; nebulizer gas (N 2 ) 30.5 psi, dry gas (N 2 ) 8.0 L/min at 220 °C, capillary voltage 4.5 kV; mass scan range: 50–1000 m / z at 1 Hz.
    The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed phase HPLC ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution From ZORBAX Eclipse Plus C18 to sub 2 micron UHPLC columms a wide selection of stationary phases and particle sizes allows you to fine tune your selectivity to match your application
    https://www.bioz.com/result/zorbax eclipse xdb c18 column/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zorbax eclipse xdb c18 column - by Bioz Stars, 2021-09
    86/100 stars

    Images

    1) Product Images from "Combining HPLC-DAD-QTOF-MS and HPLC-SPE-NMR to Monitor In Vitro Vitetrifolin D Phase I and II Metabolism"

    Article Title: Combining HPLC-DAD-QTOF-MS and HPLC-SPE-NMR to Monitor In Vitro Vitetrifolin D Phase I and II Metabolism

    Journal: Metabolites

    doi: 10.3390/metabo11080529

    HPLC-QTOF-MS-derived base peak chromatogram of the HLM incubation supernatant featuring the parent compound vitetrifolin D (VD) and its phase I metabolites M1–M22. Experimental conditions HPLC: column: Zorbax Eclipse XDB-C18, 3 × 100 mm, 3.5 µm; mobile phase: A: H 2 O, B: acetonitrile, gradient: 0 min: 80% A, 5 min: 80% A, 8 min: 65% A, 35 min: 2% A; temp: 25 °C; flow rate 0.3 mL/min; injection volume:10 µL. HR-MS: ESI, positive mode; nebulizer gas (N 2 ) 30.5 psi, dry gas (N 2 ) 8.0 L/min at 220 °C, capillary voltage 4.5 kV; mass scan range: 50–1000 m / z at 1 Hz.
    Figure Legend Snippet: HPLC-QTOF-MS-derived base peak chromatogram of the HLM incubation supernatant featuring the parent compound vitetrifolin D (VD) and its phase I metabolites M1–M22. Experimental conditions HPLC: column: Zorbax Eclipse XDB-C18, 3 × 100 mm, 3.5 µm; mobile phase: A: H 2 O, B: acetonitrile, gradient: 0 min: 80% A, 5 min: 80% A, 8 min: 65% A, 35 min: 2% A; temp: 25 °C; flow rate 0.3 mL/min; injection volume:10 µL. HR-MS: ESI, positive mode; nebulizer gas (N 2 ) 30.5 psi, dry gas (N 2 ) 8.0 L/min at 220 °C, capillary voltage 4.5 kV; mass scan range: 50–1000 m / z at 1 Hz.

    Techniques Used: High Performance Liquid Chromatography, Derivative Assay, Incubation, Injection

    2) Product Images from "Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs"

    Article Title: Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs

    Journal: Molecules

    doi: 10.3390/molecules25010156

    Chromatography isolation, purification and characterization of Gm -SGPP. ( A ) Anion-exchange chromatography of crude sample on Q Sepharose Fast Flow column (QFF) (3 × 5 mL). Flow rate, 1 mL/min; fraction size, 4 mL. ( B ) Gel filtration of fraction Q-4 on Sephacryl S-300 column (1.6 × 100 cm). Flow rate, 1 mL/min; fraction size, 4 mL. ( C ) High-performance size exclusion chromatography (HPSEC) profiles of Gm -SGPP using a TSK-GEL G4000PWXL column (30 cm × 7.8 mm). Flowing phase, 0.2 M NaCl; flow rate, 0.5 mL/min; column temperature, 40 °C ( D ) High-performance liquid chromatograph (HPLC) of sialic acid using a ZORBAX SB-C18 column (4.6 mm × 150 mm). Flowing phase, 5% acetonitrile–ultrapure water; flow rate, 1 mL/min; column temperature, 35 °C ( E ) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)of Gm -SGPP. 1, green for phosphorus staining; 2, Sudan black B for lipid staining; 3, periodic acid/Schiff for carbohydrate staining; 4, Coomassie Brilliant Blue for protein staining.
    Figure Legend Snippet: Chromatography isolation, purification and characterization of Gm -SGPP. ( A ) Anion-exchange chromatography of crude sample on Q Sepharose Fast Flow column (QFF) (3 × 5 mL). Flow rate, 1 mL/min; fraction size, 4 mL. ( B ) Gel filtration of fraction Q-4 on Sephacryl S-300 column (1.6 × 100 cm). Flow rate, 1 mL/min; fraction size, 4 mL. ( C ) High-performance size exclusion chromatography (HPSEC) profiles of Gm -SGPP using a TSK-GEL G4000PWXL column (30 cm × 7.8 mm). Flowing phase, 0.2 M NaCl; flow rate, 0.5 mL/min; column temperature, 40 °C ( D ) High-performance liquid chromatograph (HPLC) of sialic acid using a ZORBAX SB-C18 column (4.6 mm × 150 mm). Flowing phase, 5% acetonitrile–ultrapure water; flow rate, 1 mL/min; column temperature, 35 °C ( E ) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)of Gm -SGPP. 1, green for phosphorus staining; 2, Sudan black B for lipid staining; 3, periodic acid/Schiff for carbohydrate staining; 4, Coomassie Brilliant Blue for protein staining.

    Techniques Used: Chromatography, Isolation, Purification, Filtration, Size-exclusion Chromatography, High Performance Liquid Chromatography, Polyacrylamide Gel Electrophoresis, Staining

    3) Product Images from "Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD"

    Article Title: Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD

    Journal: Journal of Biomedicine and Biotechnology

    doi: 10.1155/2011/917232

    LC-MS chromatograms of mixed standards (a) and (b) in negative-ion mode. HPLC was performed on a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μ m) at the column temperature of 30°C. The separation was achieved using the following gradient program: 0–40 min (10%~50% methanol), 40–60 min (50%~100% methanol). The flowrate was at 0.8 mL/min and the sample injection volume was 5.0 μ L. Peak assignments: (a) canrenone; (b) 11- α -hydroxy-canrenone. MS spectrograms (A) and (B) stand for the molecular weight of the peak (a) and (b) at 357.44 and 341.40 amu, respectively.
    Figure Legend Snippet: LC-MS chromatograms of mixed standards (a) and (b) in negative-ion mode. HPLC was performed on a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μ m) at the column temperature of 30°C. The separation was achieved using the following gradient program: 0–40 min (10%~50% methanol), 40–60 min (50%~100% methanol). The flowrate was at 0.8 mL/min and the sample injection volume was 5.0 μ L. Peak assignments: (a) canrenone; (b) 11- α -hydroxy-canrenone. MS spectrograms (A) and (B) stand for the molecular weight of the peak (a) and (b) at 357.44 and 341.40 amu, respectively.

    Techniques Used: Liquid Chromatography with Mass Spectroscopy, High Performance Liquid Chromatography, Injection, Mass Spectrometry, Molecular Weight

    4) Product Images from "Neuropeptides isotocin and arginine vasotocin in urophysis of three fish species"

    Article Title: Neuropeptides isotocin and arginine vasotocin in urophysis of three fish species

    Journal: Fish Physiology and Biochemistry

    doi: 10.1007/s10695-012-9746-6

    Representative LC-ESI MS/MS MRM (multi reaction monitoring) chromatogram of urophysial sample of round goby. Chromatographic conditions: Agilent Zorbax Extend Plus C18 column (50 mm × 2.1 mm I.D., 1.8 μm particle); elution: solvent A (0.1 % acetic acid in H 2 O), solvent B [0.1 % acetic acid in acetonitrile: H 2 O (3:1)], a gradient elution was used starting from 5 to 30 % B in 5.3 min; flow rate 0.6 mL/min; column temperature 20 °C; injection volume 5 μL; the monitored mass transitions for AVT were set at m/z 525.5 → 517.2 and for IT were set at m/z 483.7 → 136.1
    Figure Legend Snippet: Representative LC-ESI MS/MS MRM (multi reaction monitoring) chromatogram of urophysial sample of round goby. Chromatographic conditions: Agilent Zorbax Extend Plus C18 column (50 mm × 2.1 mm I.D., 1.8 μm particle); elution: solvent A (0.1 % acetic acid in H 2 O), solvent B [0.1 % acetic acid in acetonitrile: H 2 O (3:1)], a gradient elution was used starting from 5 to 30 % B in 5.3 min; flow rate 0.6 mL/min; column temperature 20 °C; injection volume 5 μL; the monitored mass transitions for AVT were set at m/z 525.5 → 517.2 and for IT were set at m/z 483.7 → 136.1

    Techniques Used: Mass Spectrometry, Flow Cytometry, Injection

    Representative HPLC-FL chromatograms of a urophysial sample of round goby and b the same sample spiked with standard AVT (6.6 pmol/mL) and IT (6.8 pmol/mL). Chromatographic conditions: Agilent Zorbax Eclipse XDB-C18 column (150 mm × 4.6 mm I.D., 5 μm particle); elution: solvent A (0.1 % TFA in H 2 O), solvent B [0.1 % TFA in acetonitrile : H 2 O (3:1)], linear gradient 45–80 % of eluent B in 12 min; flow rate 1 mL/min; column temperature 20 °C; injection volume 40 μL; detection: FL, excitation 470 nm, emission 530 nm
    Figure Legend Snippet: Representative HPLC-FL chromatograms of a urophysial sample of round goby and b the same sample spiked with standard AVT (6.6 pmol/mL) and IT (6.8 pmol/mL). Chromatographic conditions: Agilent Zorbax Eclipse XDB-C18 column (150 mm × 4.6 mm I.D., 5 μm particle); elution: solvent A (0.1 % TFA in H 2 O), solvent B [0.1 % TFA in acetonitrile : H 2 O (3:1)], linear gradient 45–80 % of eluent B in 12 min; flow rate 1 mL/min; column temperature 20 °C; injection volume 40 μL; detection: FL, excitation 470 nm, emission 530 nm

    Techniques Used: High Performance Liquid Chromatography, Flow Cytometry, Injection

    5) Product Images from "The Identification of a SIRT6 Activator from Brown Algae Fucus distichus"

    Article Title: The Identification of a SIRT6 Activator from Brown Algae Fucus distichus

    Journal: Marine Drugs

    doi: 10.3390/md15060190

    HPLC chromatogram of F. distichus and its separation into eight fractions using Zorbax Eclipse XDB-C18 column (4.6 mm × 50 mm, 1.8 µm). The collected fractions: F1 = 0.4–0.5 min; F7 = 5.4–5.5 min.
    Figure Legend Snippet: HPLC chromatogram of F. distichus and its separation into eight fractions using Zorbax Eclipse XDB-C18 column (4.6 mm × 50 mm, 1.8 µm). The collected fractions: F1 = 0.4–0.5 min; F7 = 5.4–5.5 min.

    Techniques Used: High Performance Liquid Chromatography

    6) Product Images from "The Identification of a SIRT6 Activator from Brown Algae Fucus distichus"

    Article Title: The Identification of a SIRT6 Activator from Brown Algae Fucus distichus

    Journal: Marine Drugs

    doi: 10.3390/md15060190

    HPLC chromatogram of F. distichus and its separation into eight fractions using Zorbax Eclipse XDB-C18 column (4.6 mm × 50 mm, 1.8 µm). The collected fractions: F1 = 0.4–0.5 min; F7 = 5.4–5.5 min.
    Figure Legend Snippet: HPLC chromatogram of F. distichus and its separation into eight fractions using Zorbax Eclipse XDB-C18 column (4.6 mm × 50 mm, 1.8 µm). The collected fractions: F1 = 0.4–0.5 min; F7 = 5.4–5.5 min.

    Techniques Used: High Performance Liquid Chromatography

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Mutually exclusive substrate selection strategy by human m3C RNA transferases METTL2A and METTL6
    Article Snippet: .. The nucleosides were separated by HPLC on a C18 column (Agilent Zorbax Eclipse Plus C18, 2.1 50 mm, 1.8 mm) and then detected by a triple-quadrupole mass spectrometer (Agilent 6495 QQQ) in positive ion multiple reaction-monitoring mode. ..

    Article Title: Improved production of 2′-fucosyllactose in engineered Saccharomyces cerevisiae expressing a putative α-1, 2-fucosyltransferase from Bacillus cereus
    Article Snippet: .. The supernatant was separated from the cell debris by centrifugation (13000×g , 10 min). and was resolved by a C18 column (ZORBAX Eclipse Plus C18 4.6 × 150 mm, Agilent) connected to a high-performance liquid chromatography (HPLC) system (Shimadzu, Kyoto, Japan) equipped with a UV detector. ..

    Article Title: The role of the electrokinetic charge of neurotrophis-based nanocarriers: protein distribution, toxicity, and oxidative stress in in vitro setting
    Article Snippet: .. HPLC (varian vista series) was performed on an Agilent Zorbax column (300A). ..

    Article Title: Phosphorus-Induced Adaptation Mechanisms of Rye Grown on Post-Flotation Copper Tailings
    Article Snippet: .. Low-molecular-weight organic acids (OAs) present in the concentrated root exudates were determined by HPLC, injecting the samples (40 μL) onto a ZORBAX Eclipse C18 column (250 mm × 4.6 mm; Agilent, Santa Clara, CA, USA) maintained in a thermostated column oven. ..

    Article Title: Urinary Malondialdehyde as a Biomarker of Type 2 Diabetes Mellitus Treatment in the Primary Care Unit of a Tertiary Care Hospital
    Article Snippet: .. HPLC (1100 Series; Agilent, Foster City, CA, USA) with diode array detector (DAD), UV detector (310 nm for Excitation wavelength and 510 nm for Emission wavelength), and Agilent ZORBAX columns (4.6 × 250 mm ID, 5 μm particle size) was set in this study. ..

    Mass Spectrometry:

    Article Title: Mutually exclusive substrate selection strategy by human m3C RNA transferases METTL2A and METTL6
    Article Snippet: .. The nucleosides were separated by HPLC on a C18 column (Agilent Zorbax Eclipse Plus C18, 2.1 50 mm, 1.8 mm) and then detected by a triple-quadrupole mass spectrometer (Agilent 6495 QQQ) in positive ion multiple reaction-monitoring mode. ..

    Centrifugation:

    Article Title: Improved production of 2′-fucosyllactose in engineered Saccharomyces cerevisiae expressing a putative α-1, 2-fucosyltransferase from Bacillus cereus
    Article Snippet: .. The supernatant was separated from the cell debris by centrifugation (13000×g , 10 min). and was resolved by a C18 column (ZORBAX Eclipse Plus C18 4.6 × 150 mm, Agilent) connected to a high-performance liquid chromatography (HPLC) system (Shimadzu, Kyoto, Japan) equipped with a UV detector. ..

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    Agilent technologies zorbax eclipse xdb c18
    Dependence of the peak area of the DPPH radical on its concentration measured at different wavelength. Chromatographic conditions: column—a <t>Zorbax</t> Eclipse <t>XDB-C18</t> (4.6 × 150 mm, 5 μm), mobile phase—methanol/water (80:30, v / v ), flow rate—1 mL/min. The presence of the trace content of DPPH-H (2,2-diphenyl-1-picrylhydrazine) at each measured sample is illustrated in red.
    Zorbax Eclipse Xdb C18, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zorbax eclipse xdb c18/product/Agilent technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zorbax eclipse xdb c18 - by Bioz Stars, 2021-09
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    Dependence of the peak area of the DPPH radical on its concentration measured at different wavelength. Chromatographic conditions: column—a Zorbax Eclipse XDB-C18 (4.6 × 150 mm, 5 μm), mobile phase—methanol/water (80:30, v / v ), flow rate—1 mL/min. The presence of the trace content of DPPH-H (2,2-diphenyl-1-picrylhydrazine) at each measured sample is illustrated in red.

    Journal: Molecules

    Article Title: The [DPPH●/DPPH-H]-HPLC-DAD Method on Tracking the Antioxidant Activity of Pure Antioxidants and Goutweed (Aegopodium podagraria L.) Hydroalcoholic Extracts

    doi: 10.3390/molecules25246005

    Figure Lengend Snippet: Dependence of the peak area of the DPPH radical on its concentration measured at different wavelength. Chromatographic conditions: column—a Zorbax Eclipse XDB-C18 (4.6 × 150 mm, 5 μm), mobile phase—methanol/water (80:30, v / v ), flow rate—1 mL/min. The presence of the trace content of DPPH-H (2,2-diphenyl-1-picrylhydrazine) at each measured sample is illustrated in red.

    Article Snippet: The column was a Zorbax Eclipse XDB-C18 (Agilent Technologies, Santa Clara, CA, USA); (150 mm × 4.6 mm I.D., 5 µm).

    Techniques: Concentration Assay

    Chromatography isolation, purification and characterization of Gm -SGPP. ( A ) Anion-exchange chromatography of crude sample on Q Sepharose Fast Flow column (QFF) (3 × 5 mL). Flow rate, 1 mL/min; fraction size, 4 mL. ( B ) Gel filtration of fraction Q-4 on Sephacryl S-300 column (1.6 × 100 cm). Flow rate, 1 mL/min; fraction size, 4 mL. ( C ) High-performance size exclusion chromatography (HPSEC) profiles of Gm -SGPP using a TSK-GEL G4000PWXL column (30 cm × 7.8 mm). Flowing phase, 0.2 M NaCl; flow rate, 0.5 mL/min; column temperature, 40 °C ( D ) High-performance liquid chromatograph (HPLC) of sialic acid using a ZORBAX SB-C18 column (4.6 mm × 150 mm). Flowing phase, 5% acetonitrile–ultrapure water; flow rate, 1 mL/min; column temperature, 35 °C ( E ) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)of Gm -SGPP. 1, green for phosphorus staining; 2, Sudan black B for lipid staining; 3, periodic acid/Schiff for carbohydrate staining; 4, Coomassie Brilliant Blue for protein staining.

    Journal: Molecules

    Article Title: Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs

    doi: 10.3390/molecules25010156

    Figure Lengend Snippet: Chromatography isolation, purification and characterization of Gm -SGPP. ( A ) Anion-exchange chromatography of crude sample on Q Sepharose Fast Flow column (QFF) (3 × 5 mL). Flow rate, 1 mL/min; fraction size, 4 mL. ( B ) Gel filtration of fraction Q-4 on Sephacryl S-300 column (1.6 × 100 cm). Flow rate, 1 mL/min; fraction size, 4 mL. ( C ) High-performance size exclusion chromatography (HPSEC) profiles of Gm -SGPP using a TSK-GEL G4000PWXL column (30 cm × 7.8 mm). Flowing phase, 0.2 M NaCl; flow rate, 0.5 mL/min; column temperature, 40 °C ( D ) High-performance liquid chromatograph (HPLC) of sialic acid using a ZORBAX SB-C18 column (4.6 mm × 150 mm). Flowing phase, 5% acetonitrile–ultrapure water; flow rate, 1 mL/min; column temperature, 35 °C ( E ) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)of Gm -SGPP. 1, green for phosphorus staining; 2, Sudan black B for lipid staining; 3, periodic acid/Schiff for carbohydrate staining; 4, Coomassie Brilliant Blue for protein staining.

    Article Snippet: Monosaccharide composition was analyzed through the PMP precolumn derivatization with HPLC (PMP-HPLC) method using a ZORBAX Eclipse XDB-C18 column (Agilent, USA) [ ].

    Techniques: Chromatography, Isolation, Purification, Filtration, Size-exclusion Chromatography, High Performance Liquid Chromatography, Polyacrylamide Gel Electrophoresis, Staining

    LC-MS chromatograms of mixed standards (a) and (b) in negative-ion mode. HPLC was performed on a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μ m) at the column temperature of 30°C. The separation was achieved using the following gradient program: 0–40 min (10%~50% methanol), 40–60 min (50%~100% methanol). The flowrate was at 0.8 mL/min and the sample injection volume was 5.0 μ L. Peak assignments: (a) canrenone; (b) 11- α -hydroxy-canrenone. MS spectrograms (A) and (B) stand for the molecular weight of the peak (a) and (b) at 357.44 and 341.40 amu, respectively.

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD

    doi: 10.1155/2011/917232

    Figure Lengend Snippet: LC-MS chromatograms of mixed standards (a) and (b) in negative-ion mode. HPLC was performed on a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μ m) at the column temperature of 30°C. The separation was achieved using the following gradient program: 0–40 min (10%~50% methanol), 40–60 min (50%~100% methanol). The flowrate was at 0.8 mL/min and the sample injection volume was 5.0 μ L. Peak assignments: (a) canrenone; (b) 11- α -hydroxy-canrenone. MS spectrograms (A) and (B) stand for the molecular weight of the peak (a) and (b) at 357.44 and 341.40 amu, respectively.

    Article Snippet: The separation was performed using a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μ m) supplied by Agilent Technologies, USA.

    Techniques: Liquid Chromatography with Mass Spectroscopy, High Performance Liquid Chromatography, Injection, Mass Spectrometry, Molecular Weight

    UHPLC-MS/MS chromatograms for some sets of the 5-AIQC-tagged amino analytes having the same pseudomolecular ions (m/z at unit resolution). ( A ) ion m/z 260 (A1: sarcosine; A2: β-alanine; A3: L-alanine; A4: 2-amino-2-methyl-1-propanol); ion m/z 274 (A21: γ-aminobutyric acid; A22: DL-3-aminoisobutyric acid; A23: 2-aminoisobutyric acid; A24: L-2-aminobutyric acid); ion m/z 302 (A31: trans -4-hydroxy-L-proline; A32: 6-aminocaproic acid; A33: L-isoleucine; A34: L-leucine; A35: L-norleucine); ion m/z 324 (A41: (±)-octopamine; A42: dopamine; A43: 3-hydroxyanthranilic acid; A44: 3-aminosalicylic acid); ion m/z 340 (A51: L-cysteic acid; A52: 3-methyl-L-histidine; A53: 1-methyl-L-histidine; A54: (−)-norepinephrine; A55: 5-hydroxydopamine). ( B ) ion m/z 288 (B1: 5-aminovaleric acid; B2: L-valine; B3: L-norvaline); ion m/z 308 (B21: tyramine; B22: 3-aminobenzoic acid; B23: 4-aminobenzoic acid); ion m/z 318 (B31: L-glutamic acid; B32: o-acetyl-L-serine; B33: 4-hydroxy-L-isoleucine); ion m/z 350 (B41: D-mannosamine; B42: D-(+)-glucosamine; B43: D-(+)-galactosamine); ion m/z 373 (B51: asymmetric dimethylarginine; B52: cysteamine; B53: Ala-Leu). ( C ) ion m/z 280 (C1: hypotaurine; C2: 4-aminophenol); ion m/z 282 (C21: histamine; C22: cystathionine); ion m/z 290 (C31: D-homoserine; C32: L-threonine); ion m/z 334 (C41: 1-deoxynojirimycin; C42: DL-ethionine); ion m/z 336 (C51: DL-methionine sulfoxide; C52: DL-phenylalanine); ion m/z 352 (C61: DL-methionine sulfone; C62: L-tyrosine); ion m/z 359 (C71: L-homoarginine; C72: Nα-acetyl-L-lysine); ion m/z 208 (C81: 1,3-diaminopropane; C82: 1,2-diaminopropane).

    Journal: Scientific Reports

    Article Title: Simultaneous Quantification of Amino Metabolites in Multiple Metabolic Pathways Using Ultra-High Performance Liquid Chromatography with Tandem-mass Spectrometry

    doi: 10.1038/s41598-017-01435-7

    Figure Lengend Snippet: UHPLC-MS/MS chromatograms for some sets of the 5-AIQC-tagged amino analytes having the same pseudomolecular ions (m/z at unit resolution). ( A ) ion m/z 260 (A1: sarcosine; A2: β-alanine; A3: L-alanine; A4: 2-amino-2-methyl-1-propanol); ion m/z 274 (A21: γ-aminobutyric acid; A22: DL-3-aminoisobutyric acid; A23: 2-aminoisobutyric acid; A24: L-2-aminobutyric acid); ion m/z 302 (A31: trans -4-hydroxy-L-proline; A32: 6-aminocaproic acid; A33: L-isoleucine; A34: L-leucine; A35: L-norleucine); ion m/z 324 (A41: (±)-octopamine; A42: dopamine; A43: 3-hydroxyanthranilic acid; A44: 3-aminosalicylic acid); ion m/z 340 (A51: L-cysteic acid; A52: 3-methyl-L-histidine; A53: 1-methyl-L-histidine; A54: (−)-norepinephrine; A55: 5-hydroxydopamine). ( B ) ion m/z 288 (B1: 5-aminovaleric acid; B2: L-valine; B3: L-norvaline); ion m/z 308 (B21: tyramine; B22: 3-aminobenzoic acid; B23: 4-aminobenzoic acid); ion m/z 318 (B31: L-glutamic acid; B32: o-acetyl-L-serine; B33: 4-hydroxy-L-isoleucine); ion m/z 350 (B41: D-mannosamine; B42: D-(+)-glucosamine; B43: D-(+)-galactosamine); ion m/z 373 (B51: asymmetric dimethylarginine; B52: cysteamine; B53: Ala-Leu). ( C ) ion m/z 280 (C1: hypotaurine; C2: 4-aminophenol); ion m/z 282 (C21: histamine; C22: cystathionine); ion m/z 290 (C31: D-homoserine; C32: L-threonine); ion m/z 334 (C41: 1-deoxynojirimycin; C42: DL-ethionine); ion m/z 336 (C51: DL-methionine sulfoxide; C52: DL-phenylalanine); ion m/z 352 (C61: DL-methionine sulfone; C62: L-tyrosine); ion m/z 359 (C71: L-homoarginine; C72: Nα-acetyl-L-lysine); ion m/z 208 (C81: 1,3-diaminopropane; C82: 1,2-diaminopropane).

    Article Snippet: The 5-AIQC-tagged samples (1 μL) were individually injected on an UHPLC column (Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD, 2.1 × 100 mm, 1.8 μm) with its temperature set to 50 °C.

    Techniques: Mass Spectrometry

    UHPLC-MS/MS chromatograms for the 5-AIQC-tagged oxidation-prone amino analytes including ( a ) these containing thiol and disulfide groups and ( b ) the aromatic metabolites from three aromatic amino acids (phenylalanine, tyrosine and tryptophan).

    Journal: Scientific Reports

    Article Title: Simultaneous Quantification of Amino Metabolites in Multiple Metabolic Pathways Using Ultra-High Performance Liquid Chromatography with Tandem-mass Spectrometry

    doi: 10.1038/s41598-017-01435-7

    Figure Lengend Snippet: UHPLC-MS/MS chromatograms for the 5-AIQC-tagged oxidation-prone amino analytes including ( a ) these containing thiol and disulfide groups and ( b ) the aromatic metabolites from three aromatic amino acids (phenylalanine, tyrosine and tryptophan).

    Article Snippet: The 5-AIQC-tagged samples (1 μL) were individually injected on an UHPLC column (Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD, 2.1 × 100 mm, 1.8 μm) with its temperature set to 50 °C.

    Techniques: Mass Spectrometry

    UHPLC-MS/MS chromatograms for the 5-AIQC-tagged amino metabolites in multiple metabolic pathways including ( a ) protein biosynthesis/degradation, ( b ) urea cycle, ( c ) folate-associated homocysteine metabolism, ( d ) biosynthesis of monoamine neurotransmitters and ( e ) tryptophan-mediated kynurenine pathway.

    Journal: Scientific Reports

    Article Title: Simultaneous Quantification of Amino Metabolites in Multiple Metabolic Pathways Using Ultra-High Performance Liquid Chromatography with Tandem-mass Spectrometry

    doi: 10.1038/s41598-017-01435-7

    Figure Lengend Snippet: UHPLC-MS/MS chromatograms for the 5-AIQC-tagged amino metabolites in multiple metabolic pathways including ( a ) protein biosynthesis/degradation, ( b ) urea cycle, ( c ) folate-associated homocysteine metabolism, ( d ) biosynthesis of monoamine neurotransmitters and ( e ) tryptophan-mediated kynurenine pathway.

    Article Snippet: The 5-AIQC-tagged samples (1 μL) were individually injected on an UHPLC column (Agilent Zorbax Eclipse XDB-C18 Rapid Resolution HD, 2.1 × 100 mm, 1.8 μm) with its temperature set to 50 °C.

    Techniques: Mass Spectrometry