zona pellucida 1  (Thermo Fisher)


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    Name:
    TZ 28 Zonal Rotor
    Description:
    Increase lab productivity with the Thermo Scientific TZ 28 Zonal Rotor delivering maximum flexibility at 28000rpm
    Catalog Number:
    52358
    Price:
    None
    Category:
    Instruments and Equipment
    Applications:
    Centrifuges|Lab Equipment
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    Structured Review

    Thermo Fisher zona pellucida 1
    Increase lab productivity with the Thermo Scientific TZ 28 Zonal Rotor delivering maximum flexibility at 28000rpm
    https://www.bioz.com/result/zona pellucida 1/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zona pellucida 1 - by Bioz Stars, 2021-04
    93/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection
    Article Snippet: .. 3-D aggregates and 2-D monolayer cells were stained with antibodies specific for scavenger receptor class B member 1 (SR-BI) (BD Biosciences, Franklin Lakes, NJ), CD81 (AbD Serotec, Raleigh, NC), CLDN1 (Abnova, Taipei, Taiwan), CD26 (Abcam, Cambridge, UK), β-Catenin (Zymed, San Francisco, CA), E-cadherin (Zymed), zona occludens 1 (ZO-1) (Zymed), Occludin (Zymed) or HCV E2 (C1 [ ]) overnight at 4°C, followed by incubation with a 1:1,000 dilution of an appropriate Alexa555-conjugated secondary antibody (Molecular Probes, Carlsbad, CA) for 1 h at room temperature. .. Cell nuclei were stained by Hoechst dye.

    Incubation:

    Article Title: Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection
    Article Snippet: .. 3-D aggregates and 2-D monolayer cells were stained with antibodies specific for scavenger receptor class B member 1 (SR-BI) (BD Biosciences, Franklin Lakes, NJ), CD81 (AbD Serotec, Raleigh, NC), CLDN1 (Abnova, Taipei, Taiwan), CD26 (Abcam, Cambridge, UK), β-Catenin (Zymed, San Francisco, CA), E-cadherin (Zymed), zona occludens 1 (ZO-1) (Zymed), Occludin (Zymed) or HCV E2 (C1 [ ]) overnight at 4°C, followed by incubation with a 1:1,000 dilution of an appropriate Alexa555-conjugated secondary antibody (Molecular Probes, Carlsbad, CA) for 1 h at room temperature. .. Cell nuclei were stained by Hoechst dye.

    Article Title: Glibenclamide reduces inflammation, vasogenic edema, and caspase-3 activation after subarachnoid hemorrhage
    Article Snippet: Cryosections (10 μm) were postfixed with 4% formaldehyde and immunolabeled using standard techniques. .. After permeabilizing (0.3% Triton X-100 for 10 mins), sections were blocked (2% donkey serum for 1 h; Sigma; D-9663), then incubated with primary antibody directed against: SUR1 (1:200; SC-5789; Santa Cruz Biotechnology, Santa Cruz, CA, USA); NeuN (1:100; MAB377; Chemicon, Billerica, MA, USA); glial fibrillary acidic protein (GFAP; 1:500; CY3 conjugated; C-9205; Sigma); vonWillebrand factor (1:200; F3520; Sigma); tumor-necrosis factor- α (TNF α ) (1:100; Santa Cruz Biotechnology); p65 (1:100; Santa Cruz Biotechnology); FITC-conjugated immunoglobulin G (IgG; 1:200; Santa Cruz Biotechnology); zona occludens 1 (ZO-1; 1:100; Zymed Laboratories, San Francisco, CA, USA). .. Fluorescent-labeled, species appropriate secondary antibodies (Molecular Probes, Carlsbad, CA, USA) were used for visualization.

    Immunostaining:

    Article Title:
    Article Snippet: The glial cells were obtained from neonatal CD-1 mice, by published procedures ( ; ). .. The purity of ECs was checked by positive immunostaining for factor VIII (Millipore), zona occludens-1 (Invitrogen, Carlsbad, CA), and claudin-5 (Abcam); of PCs for α -SMA, CD13, PDGFR- β ; and of glial cells for glial fibrillary acidic protein (Abcam). ..

    Immunofluorescence:

    Article Title: A responsive human triple‐culture model of the air–blood barrier: incorporation of different macrophage phenotypes) A responsive human triple‐culture model of the air–blood barrier: incorporation of different macrophage phenotypes
    Article Snippet: 2.4 Characterization of THP‐1 cells differentiated into various subtypes After 48 h of incubation with the differentiation cocktails, the morphology of the cells was examined by means of light microscopy (All‐in‐One Fluorescence Microscope, BIOREVO BZ‐9000, Keyence) and fluorescence microscopy (CD68) (DeltaVision, Applied Precision). .. 2.5 Immunofluorescence (IF) for marker proteins IF was performed to label marker proteins for macrophages (CD68, Dako, cat. no. M0718; or CD11a, Becton‐Dickinson, cat. no. 610826) or tight junctions of epithelial cells, such as zona occludens‐1 (ZO‐1; Zymed, cat. no. 61–7300). ..

    Marker:

    Article Title: A responsive human triple‐culture model of the air–blood barrier: incorporation of different macrophage phenotypes) A responsive human triple‐culture model of the air–blood barrier: incorporation of different macrophage phenotypes
    Article Snippet: 2.4 Characterization of THP‐1 cells differentiated into various subtypes After 48 h of incubation with the differentiation cocktails, the morphology of the cells was examined by means of light microscopy (All‐in‐One Fluorescence Microscope, BIOREVO BZ‐9000, Keyence) and fluorescence microscopy (CD68) (DeltaVision, Applied Precision). .. 2.5 Immunofluorescence (IF) for marker proteins IF was performed to label marker proteins for macrophages (CD68, Dako, cat. no. M0718; or CD11a, Becton‐Dickinson, cat. no. 610826) or tight junctions of epithelial cells, such as zona occludens‐1 (ZO‐1; Zymed, cat. no. 61–7300). ..

    Article Title: Lung Self-Assembly Is Modulated by Tissue Surface Tensions
    Article Snippet: .. Cryostat sections were permeabilized with 0.1% Triton-X100, rinsed with PBS, blocked using CAS Block blocking buffer (Zymed, San Francisco, CA), and exposed to the primary antibody for 1 hour (laminin α [ab30320; Abcam], PECAM-1 (platelet endothelial cell adhesion molecule-1) [553370; BD Pharmingen, San Diego, CA], zona occludens [ZO]–1 [40–2300; Zymed]; SPC [07-647; Upstate, Lake Placid, NY], FN [ab23750; Abcam], collagen I [ab21286; Abcam], activate caspase 3 [AB3623; Chemicon, Temecula, CA]), smooth muscle actin (SMA), Golgi marker (GM130), per the manufacturers' instructions. .. After washing with PBS, tissues were exposed to the appropriate secondary Cy3 or AlexaFluor 488 fluorescent antibody (Chemicon and Molecular Probes/Invitrogen) for 1 hour.

    SDS Page:

    Article Title: VX765 Attenuates Pyroptosis and HMGB1/TLR4/NF-κB Pathways to Improve Functional Outcomes in TBI Mice
    Article Snippet: After the membranes were blocked with 5% skimmed milk, Tris-buffered saline with tween-20 (TBST) was incubated at room temperature for 2 h, and the membrane was incubated with the corresponding main antibody overnight at 4°C. .. Then, 8% SDS-PAGE gel was used for detection on NLRP1 (1 : 1000, Abcam), NLRP3 (1 : 1000, CST), NLRC4 (1 : 1000, Abcam), and zona pellucida 1 (ZO-1, 1 : 1000, Invitrogen), and 10% SDS-acrylamide gel was applied for AIM2 (1 : 1000, Abcam), GSDMD (1 : 1000, Abcam), and NF-κ B (1 : 1000, CST). .. Twelve percent SDS-acrylamide gels were used to detect p-Iκ Bα (1 : 1000, CST), Iκ Bα (1 : 1000, CST), ASC (1 : 1000, CST), caspase-1 (1 : 1000, Proteintech), cleaved-caspase-1 (1 : 1000, CST), IL-1β (1 : 1000, Abcam), IL-18 (1 : 1000, Abcam), HMGB1 (1 : 1000, Proteintech), TLR4 (1 : 1000, Proteintech), and cleaved-caspase-3 (1 : 1000, Abcam).

    Blocking Assay:

    Article Title: Lung Self-Assembly Is Modulated by Tissue Surface Tensions
    Article Snippet: .. Cryostat sections were permeabilized with 0.1% Triton-X100, rinsed with PBS, blocked using CAS Block blocking buffer (Zymed, San Francisco, CA), and exposed to the primary antibody for 1 hour (laminin α [ab30320; Abcam], PECAM-1 (platelet endothelial cell adhesion molecule-1) [553370; BD Pharmingen, San Diego, CA], zona occludens [ZO]–1 [40–2300; Zymed]; SPC [07-647; Upstate, Lake Placid, NY], FN [ab23750; Abcam], collagen I [ab21286; Abcam], activate caspase 3 [AB3623; Chemicon, Temecula, CA]), smooth muscle actin (SMA), Golgi marker (GM130), per the manufacturers' instructions. .. After washing with PBS, tissues were exposed to the appropriate secondary Cy3 or AlexaFluor 488 fluorescent antibody (Chemicon and Molecular Probes/Invitrogen) for 1 hour.

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  • 85
    Thermo Fisher gene exp zp1 hs01399328 m1
    Human embryonic genome activation and pluripotency program initiation. Quantitative real-time PCR analysis of ( A ) genes that are actively transcribed from the embryonic genome starting at the 2-cell ( RBBP6, FAM90A1, RFPL4B ), 4-cell ( L1TD1 ) and 6- to 8-cell ( ZSCAN4, H2AFZ ) stage, ( B ) genes involved in the initiation of the pluripotency program ( POU5F1, TDGF1, NANOG, SOX2 and KLF4 ) and ( C ) maternally inherited mRNAs ( FIGLA, <t>ZP1</t> ). Blue bars, control culture conditions; red bars, α-amanitin-supplemented culture medium. Units on the y -axis indicate fold changes of expression as compared with MII (unit of expression). Error bars indicate s.e.
    Gene Exp Zp1 Hs01399328 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp zp1 hs01399328 m1/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp zp1 hs01399328 m1 - by Bioz Stars, 2021-04
    85/100 stars
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    93
    Thermo Fisher gene exp zpld1 hs00604192 m1
    mRNA expression levels of DNA microarray validation genes. To validate the reliability of the results obtained from the DNA microarray, the expression levels of EMX2, PTPRD, STEAP1, <t>ZPLD1,</t> CDH1, NR1H4 and EHF genes were analyzed by qRT-PCR. Validation genes were selected among those that meet the following requirements: i) their expression was altered by some of the mutations compared to the WT condition and, ii) its expression was also modified by the silencing of CLCN5 and totally or partially restored by ClC-5 WT re-introduction. All genes showed an expression pattern that correlated with that observed in the DNA microarray.
    Gene Exp Zpld1 Hs00604192 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp zpld1 hs00604192 m1/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp zpld1 hs00604192 m1 - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher zona pellucida 1
    mRNA expression levels of DNA microarray validation genes. To validate the reliability of the results obtained from the DNA microarray, the expression levels of EMX2, PTPRD, STEAP1, <t>ZPLD1,</t> CDH1, NR1H4 and EHF genes were analyzed by qRT-PCR. Validation genes were selected among those that meet the following requirements: i) their expression was altered by some of the mutations compared to the WT condition and, ii) its expression was also modified by the silencing of CLCN5 and totally or partially restored by ClC-5 WT re-introduction. All genes showed an expression pattern that correlated with that observed in the DNA microarray.
    Zona Pellucida 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zona pellucida 1/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zona pellucida 1 - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Human embryonic genome activation and pluripotency program initiation. Quantitative real-time PCR analysis of ( A ) genes that are actively transcribed from the embryonic genome starting at the 2-cell ( RBBP6, FAM90A1, RFPL4B ), 4-cell ( L1TD1 ) and 6- to 8-cell ( ZSCAN4, H2AFZ ) stage, ( B ) genes involved in the initiation of the pluripotency program ( POU5F1, TDGF1, NANOG, SOX2 and KLF4 ) and ( C ) maternally inherited mRNAs ( FIGLA, ZP1 ). Blue bars, control culture conditions; red bars, α-amanitin-supplemented culture medium. Units on the y -axis indicate fold changes of expression as compared with MII (unit of expression). Error bars indicate s.e.

    Journal: Development (Cambridge, England)

    Article Title: Waves of early transcriptional activation and pluripotency program initiation during human preimplantation development

    doi: 10.1242/dev.064741

    Figure Lengend Snippet: Human embryonic genome activation and pluripotency program initiation. Quantitative real-time PCR analysis of ( A ) genes that are actively transcribed from the embryonic genome starting at the 2-cell ( RBBP6, FAM90A1, RFPL4B ), 4-cell ( L1TD1 ) and 6- to 8-cell ( ZSCAN4, H2AFZ ) stage, ( B ) genes involved in the initiation of the pluripotency program ( POU5F1, TDGF1, NANOG, SOX2 and KLF4 ) and ( C ) maternally inherited mRNAs ( FIGLA, ZP1 ). Blue bars, control culture conditions; red bars, α-amanitin-supplemented culture medium. Units on the y -axis indicate fold changes of expression as compared with MII (unit of expression). Error bars indicate s.e.

    Article Snippet: Real-time PCR analysis was carried out with 10 ng cDNA and the following TaqMan assays: RBBP6 (Hs00199439_m1), FAM90A1 (Hs00216400_m1), RFPL4B (Hs02518369_s1), TDGF1 (Hs02339499_g1), NANOG (Hs02387400_g1), L1TD1 (Hs01102131_m1), H2AFZ (Hs01888362_g1), ZSCAN4 (Hs00537549_m1), FIGLA (Hs01079386_m1), SOX2 (Hs01053049_s1), KLF4 (Hs00358836_m1), ZP1 (Hs01399328_m1) and POU5F1 (Hs00999634_Gh).

    Techniques: Activation Assay, Real-time Polymerase Chain Reaction, Expressing

    mRNA expression levels of DNA microarray validation genes. To validate the reliability of the results obtained from the DNA microarray, the expression levels of EMX2, PTPRD, STEAP1, ZPLD1, CDH1, NR1H4 and EHF genes were analyzed by qRT-PCR. Validation genes were selected among those that meet the following requirements: i) their expression was altered by some of the mutations compared to the WT condition and, ii) its expression was also modified by the silencing of CLCN5 and totally or partially restored by ClC-5 WT re-introduction. All genes showed an expression pattern that correlated with that observed in the DNA microarray.

    Journal: bioRxiv

    Article Title: Global gene-expression analysis reveals the molecular processes underlying ClC-5 loss-of-function in novel Dent Disease 1 cellular models

    doi: 10.1101/2020.12.16.423143

    Figure Lengend Snippet: mRNA expression levels of DNA microarray validation genes. To validate the reliability of the results obtained from the DNA microarray, the expression levels of EMX2, PTPRD, STEAP1, ZPLD1, CDH1, NR1H4 and EHF genes were analyzed by qRT-PCR. Validation genes were selected among those that meet the following requirements: i) their expression was altered by some of the mutations compared to the WT condition and, ii) its expression was also modified by the silencing of CLCN5 and totally or partially restored by ClC-5 WT re-introduction. All genes showed an expression pattern that correlated with that observed in the DNA microarray.

    Article Snippet: In order to validate the microarray analysis was used the following TaqMan probes (Applied Biosystems): STEAP1 (Hs00185180_m1), ZPLD1 (Hs00604192_m1), PTPRD (Hs00369913_m1), CDH1 (Hs01023895_m1), EMX2 (Hs00244574_m1), NR1H4 (Hs01026590_m1), EHF (Hs00171917_m1), TBP (Hs00427620_m1).

    Techniques: Expressing, Microarray, Quantitative RT-PCR, Modification