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antibody zo 1  (Proteintech)


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    Proteintech antibody zo 1
    Effects of EA on dyspepsia and intestinal barrier in rats. (A) Observation of the duodenum by TEM. The yellow arrows indicate microvilli, and the green arrows indicate mitochondria. Scale bars = 5 µm. (B) HE staining of the duodenum and stomach, and the number of eosinophils in the duodenum ( n = 3). Scale bars = 100 µm. (C) Small intestinal propulsion rate and gastric emptying rate assay; arrowheads indicate the termination of small intestinal propulsion ( n = 5). (D) MTL and GAS assay ( n = 8). (E–H) IF assay for duodenal tight junction <t>protein</t> <t>ZO‐1</t> and Occludin protein expression ( n = 3). Scale bars = 100 µm. Data are expressed as mean±SD. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Antibody Zo 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 2587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zo+1/ZO-1+Antibody/pmc13691533-145-8-12
    Average 97 stars, based on 2587 article reviews
    antibody zo 1 - by Bioz Stars, 2026-11
    97/100 stars

    Images

    1) Product Images from "Electroacupuncture Alleviates Comorbid Depression‐Like Behaviors and Dyspepsia‐Like Symptoms in Rats Accompanied by Alterations in Hippocampal Neuroinflammation and Gut Microbiota"

    Article Title: Electroacupuncture Alleviates Comorbid Depression‐Like Behaviors and Dyspepsia‐Like Symptoms in Rats Accompanied by Alterations in Hippocampal Neuroinflammation and Gut Microbiota

    Journal: Brain and Behavior

    doi: 10.1002/brb3.71854

    Effects of EA on dyspepsia and intestinal barrier in rats. (A) Observation of the duodenum by TEM. The yellow arrows indicate microvilli, and the green arrows indicate mitochondria. Scale bars = 5 µm. (B) HE staining of the duodenum and stomach, and the number of eosinophils in the duodenum ( n = 3). Scale bars = 100 µm. (C) Small intestinal propulsion rate and gastric emptying rate assay; arrowheads indicate the termination of small intestinal propulsion ( n = 5). (D) MTL and GAS assay ( n = 8). (E–H) IF assay for duodenal tight junction protein ZO‐1 and Occludin protein expression ( n = 3). Scale bars = 100 µm. Data are expressed as mean±SD. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Figure Legend Snippet: Effects of EA on dyspepsia and intestinal barrier in rats. (A) Observation of the duodenum by TEM. The yellow arrows indicate microvilli, and the green arrows indicate mitochondria. Scale bars = 5 µm. (B) HE staining of the duodenum and stomach, and the number of eosinophils in the duodenum ( n = 3). Scale bars = 100 µm. (C) Small intestinal propulsion rate and gastric emptying rate assay; arrowheads indicate the termination of small intestinal propulsion ( n = 5). (D) MTL and GAS assay ( n = 8). (E–H) IF assay for duodenal tight junction protein ZO‐1 and Occludin protein expression ( n = 3). Scale bars = 100 µm. Data are expressed as mean±SD. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Techniques Used: H&E Stain, Expressing

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    Article Title: Alkylating Agent–Induced FANCL Impairment Damages Pulmonary Arterial, Venous, and Microvascular Endothelial Cells and Predisposes to PAH/PVOD
    Article Snippet: The commercial sources of major reagents and chemicals include: Mitomycin C (MMC, S8146, Selleck, Shanghai, China), Cisplatin (CDDP, MB1055, Meilunbio, Dalian, China), SU5416 (HY‐10374, MedChemExpress, Monmouth Junction, NJ, USA), Amifostine trihydrate (S4298, Selleck, Shanghai, China). .. Primary antibodies for fibronectin (FN, #15613‐I‐AP, Proteintech, Rosemont, IL, USA), Twist (ab50887, Abcam, Cambridge, MA, USA), Vimentin (#5741, Cell Signaling Technology, Danvers, MA, USA), Snail (#ab53519, Abcam, Cambridge, MA, USA), ZO‐1 (21773‐1‐AP, proteintech, Wuhan, China), VEGFR2 (9698, CST, Boston, USA), Slug (YM8357, Immunoway, California, USA), proliferating cell nuclear antigen (PCNA, ab29, Abcam, Cambridge, MA, USA), CD31 (AF3628, R&D Systems, Minneapolis, MN, USA), Cleaved caspase3 (#9664, Cell Signaling Technology, Danvers, MA, USA), Bax (#2772S, Cell Signaling Technology, Danvers, MA, USA), B‐cell lymphoma/leukemia 2 (Bcl‐2, 13–8800, Thermo Fisher Scientific, Rockford, IL, USA), γ‐H2AX (#05‐635, EMD Millipore/Merck, USA), α‐smooth muscle actin (α‐SMA, ab124964 and ab7817, Abcam, Cambridge, MA, USA), FANCL (LS‐C807939, LifeSpan BioSciences, Seattle, WA, USA), FANCD2 (NB100‐182, Novus Biologicals, USA), general control nonderepressible 2 (GCN2, #46041, Cell Signaling Technology, Danvers, MA, USA), and β‐actin (66009‐1‐Ig, Proteintech, Rosemont, IL, USA) were used for immunoblotting, immunofluorescence (IF), and immunohistochemistry (IHC) staining. ..

    Article Title: TRPV3 drives lung injury by sensing inflammatory signals to disrupt alveolar epithelial barrier and promote inflammation.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Numbers: 82675098, 81972960, 82373929; The Lingang Laboratory Drug Development Project, Grant/Award Number: LGL-2612-09; Double First-Class University Plan, Grant/Award Number: CPU2025CXY02 Abstract Background and Purpose: Pulmonary inflammation is a central pathological feature of many respiratory diseases.. Transient receptor potential vanilloid 3 (TRPV3), a nonselective cation channel abundantly expressed in the skin, nerve and lung tissues, is involved in itch, skin inflammation and barrier regulation.. However, its function in the pulmonary system remains elusive.

    Control:

    Article Title: Alkylating Agent–Induced FANCL Impairment Damages Pulmonary Arterial, Venous, and Microvascular Endothelial Cells and Predisposes to PAH/PVOD
    Article Snippet: The commercial sources of major reagents and chemicals include: Mitomycin C (MMC, S8146, Selleck, Shanghai, China), Cisplatin (CDDP, MB1055, Meilunbio, Dalian, China), SU5416 (HY‐10374, MedChemExpress, Monmouth Junction, NJ, USA), Amifostine trihydrate (S4298, Selleck, Shanghai, China). .. Primary antibodies for fibronectin (FN, #15613‐I‐AP, Proteintech, Rosemont, IL, USA), Twist (ab50887, Abcam, Cambridge, MA, USA), Vimentin (#5741, Cell Signaling Technology, Danvers, MA, USA), Snail (#ab53519, Abcam, Cambridge, MA, USA), ZO‐1 (21773‐1‐AP, proteintech, Wuhan, China), VEGFR2 (9698, CST, Boston, USA), Slug (YM8357, Immunoway, California, USA), proliferating cell nuclear antigen (PCNA, ab29, Abcam, Cambridge, MA, USA), CD31 (AF3628, R&D Systems, Minneapolis, MN, USA), Cleaved caspase3 (#9664, Cell Signaling Technology, Danvers, MA, USA), Bax (#2772S, Cell Signaling Technology, Danvers, MA, USA), B‐cell lymphoma/leukemia 2 (Bcl‐2, 13–8800, Thermo Fisher Scientific, Rockford, IL, USA), γ‐H2AX (#05‐635, EMD Millipore/Merck, USA), α‐smooth muscle actin (α‐SMA, ab124964 and ab7817, Abcam, Cambridge, MA, USA), FANCL (LS‐C807939, LifeSpan BioSciences, Seattle, WA, USA), FANCD2 (NB100‐182, Novus Biologicals, USA), general control nonderepressible 2 (GCN2, #46041, Cell Signaling Technology, Danvers, MA, USA), and β‐actin (66009‐1‐Ig, Proteintech, Rosemont, IL, USA) were used for immunoblotting, immunofluorescence (IF), and immunohistochemistry (IHC) staining. ..

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    Effects of EA on dyspepsia and intestinal barrier in rats. (A) Observation of the duodenum by TEM. The yellow arrows indicate microvilli, and the green arrows indicate mitochondria. Scale bars = 5 µm. (B) HE staining of the duodenum and stomach, and the number of eosinophils in the duodenum ( n = 3). Scale bars = 100 µm. (C) Small intestinal propulsion rate and gastric emptying rate assay; arrowheads indicate the termination of small intestinal propulsion ( n = 5). (D) MTL and GAS assay ( n = 8). (E–H) IF assay for duodenal tight junction <t>protein</t> <t>ZO‐1</t> and Occludin protein expression ( n = 3). Scale bars = 100 µm. Data are expressed as mean±SD. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    ABCA1 deficiency disrupts the junctional integrity of the colonic mucosal barrier. (A) Representative images of immunofluorescence staining of the zippering junction with E‐cadherin. (B, C) Effects of ABCA1 silencing on cholesterol crystals (CHO)‐induced cell injury. Caco‐2 cells were transfected with siNC or siABCA1 for 24 h, and then exposed to 1 mmol/L CHO or not for 24 h. The cell viability was determined using Calcein‐AM/PI staining (B) and CCK8 assay (C). The data in panel C are expressed as mean ± SD ( n = 4; *** p < 0.001 vs . siNC without CHO by Student's t ‐test). (D) ABCA1 silencing aggravates CHO‐mediated barrier dysfunction in Caco‐2 cells. Caco‐2 cells were transfected with siNC or siABCA1 for 24 h, and then exposed to 1 mmol/L CHO for 6 h. Cellular lysates were assayed for E‐cadherin, Occludin and <t>ZO‐1</t> expression. The respective densitometric quantitations of bands are presented in the bar graphs to the right of the blots. Data are expressed as mean ± SD ( n = 3; * p < 0.05, ** p < 0.01, *** p < 0.001 vs . siNC without CHO by Student's t ‐test).
    21773 1 Ap Occludin Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of EA on dyspepsia and intestinal barrier in rats. (A) Observation of the duodenum by TEM. The yellow arrows indicate microvilli, and the green arrows indicate mitochondria. Scale bars = 5 µm. (B) HE staining of the duodenum and stomach, and the number of eosinophils in the duodenum ( n = 3). Scale bars = 100 µm. (C) Small intestinal propulsion rate and gastric emptying rate assay; arrowheads indicate the termination of small intestinal propulsion ( n = 5). (D) MTL and GAS assay ( n = 8). (E–H) IF assay for duodenal tight junction protein ZO‐1 and Occludin protein expression ( n = 3). Scale bars = 100 µm. Data are expressed as mean±SD. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Brain and Behavior

    Article Title: Electroacupuncture Alleviates Comorbid Depression‐Like Behaviors and Dyspepsia‐Like Symptoms in Rats Accompanied by Alterations in Hippocampal Neuroinflammation and Gut Microbiota

    doi: 10.1002/brb3.71854

    Figure Lengend Snippet: Effects of EA on dyspepsia and intestinal barrier in rats. (A) Observation of the duodenum by TEM. The yellow arrows indicate microvilli, and the green arrows indicate mitochondria. Scale bars = 5 µm. (B) HE staining of the duodenum and stomach, and the number of eosinophils in the duodenum ( n = 3). Scale bars = 100 µm. (C) Small intestinal propulsion rate and gastric emptying rate assay; arrowheads indicate the termination of small intestinal propulsion ( n = 5). (D) MTL and GAS assay ( n = 8). (E–H) IF assay for duodenal tight junction protein ZO‐1 and Occludin protein expression ( n = 3). Scale bars = 100 µm. Data are expressed as mean±SD. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Duodenal tissue sections were added with the primary antibody ZO‐1 (1:400, 21773‐1‐AP; Proteintech) and the primary antibody Occludin (1:400, 27260‐1‐AP; Proteintech), and incubated at 4°C overnight.

    Techniques: H&E Stain, Expressing

    BPNSs gavage ameliorated arthritis by remodeling Treg/Th17 imbalance. (A) Detailed schematic of the animal experimental procedures of CIA PBS and CIA BPNSs groups. (B) Arthritis scores in CIA mice. (C) Representative photographs and micro-CT images of paws. (D) Quantitative analyses of bone histomorphometry indexes including BMD, BV/TV, Tb.Th, Tb.Sp, and Tb.N in CIA mice. (E) Representative histology images of joints. Scale bar, 100 μm. (F) Histological scores of the joints. (G) Representative TRAP staining images of joints. Scale bar, 100 μm. (H) Quantitative analyses of TRAP staining. (I–N) Flow cytometric analysis and quantification of IL-17A⁺ Th17 cells and FOXP3⁺ Treg cells among CD4⁺ T cells, as well as the Treg/Th17 ratio, in the spleen, MLN and DLN. (O–S) Flow cytometric analysis and quantification of MDSC-like cells, G-MDSC-like cells and M-MDSC-like cells in the spleen. (T) The expression of CD11c, MHCII and CD86 of MDSC-like cells in spleen detected by flow cytometry and statistically analyzed. (U) Immunofluorescence staining of ZO-1 and Occludin in colon tissue. Scale bar, 100 μm. (V) MFI quantification of ZO-1 and Occludin after normalization. (W) Volcano plot of differentially abundant serum inflammatory proteins in CIA BPNSs versus CIA PBS , as determined by a mouse inflammation antibody array. ns, p ≥ 0.05; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Gut Microbes

    Article Title: Targeted intestinal ROS scavenging alleviated rheumatoid arthritis by microbial metabolite kynurenic acid mediated Treg/Th17 balance through AhR pathway activation

    doi: 10.1080/19490976.2026.2739669

    Figure Lengend Snippet: BPNSs gavage ameliorated arthritis by remodeling Treg/Th17 imbalance. (A) Detailed schematic of the animal experimental procedures of CIA PBS and CIA BPNSs groups. (B) Arthritis scores in CIA mice. (C) Representative photographs and micro-CT images of paws. (D) Quantitative analyses of bone histomorphometry indexes including BMD, BV/TV, Tb.Th, Tb.Sp, and Tb.N in CIA mice. (E) Representative histology images of joints. Scale bar, 100 μm. (F) Histological scores of the joints. (G) Representative TRAP staining images of joints. Scale bar, 100 μm. (H) Quantitative analyses of TRAP staining. (I–N) Flow cytometric analysis and quantification of IL-17A⁺ Th17 cells and FOXP3⁺ Treg cells among CD4⁺ T cells, as well as the Treg/Th17 ratio, in the spleen, MLN and DLN. (O–S) Flow cytometric analysis and quantification of MDSC-like cells, G-MDSC-like cells and M-MDSC-like cells in the spleen. (T) The expression of CD11c, MHCII and CD86 of MDSC-like cells in spleen detected by flow cytometry and statistically analyzed. (U) Immunofluorescence staining of ZO-1 and Occludin in colon tissue. Scale bar, 100 μm. (V) MFI quantification of ZO-1 and Occludin after normalization. (W) Volcano plot of differentially abundant serum inflammatory proteins in CIA BPNSs versus CIA PBS , as determined by a mouse inflammation antibody array. ns, p ≥ 0.05; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: For IEC-6 cells, ZO-1 (Proteintech, Cat. no. 21773-1-AP, 1:5,000) and Occludin (Proteintech, Cat. no. 27260-1-AP, 1:5,000) antibodies were used.

    Techniques: Micro-CT, Histology, Staining, Analysis, Expressing, Flow Cytometry, Immunofluorescence, Tissue, Ab Array

    KYNA exerted anti-rheumatic arthritis effects in vivo through the AhR pathway. (A) Detailed schematic of the animal experimental procedures of CIA PBS , CIA KYNA and CIA KYNA+CH-223191 groups. (B) Arthritis scores in CIA mice. (C) Representative photographs and micro-CT images of paws. (D) Quantitative analyses of bone histomorphometry indexes including BMD, BV/TV, Tb.Th, Tb.Sp, and Tb.N in CIA mice. (E) Representative HE staining images of joints. Scale bar, 100 μm. (F) Histological scores of the joints. (G) Representative TRAP staining images of joints. Scale bar, 100 μm. (H) Quantitative analyses of TRAP staining. (I–L) Flow cytometric analysis and quantification of IL-17A⁺ Th17 cells and FOXP3⁺ Treg cells among CD4⁺ T cells, as well as the Treg/Th17 ratio, in the spleen and MLN. (M–P) (M and O), Representative immunofluorescence staining of ZO-1 and Occludin in colon tissue. Scale bar, 100 μm. (N and P) Quantification of the MFI of ZO-1 and Occludin after normalization. (Q) Representative Western blot images of ZO-1 and Occludin in the presence of KYNA or KYNA + CH-223191 in IEC-6 cells. (R and S) Western blot quantification of ZO-1 and Occludin protein levels (normalized to β-actin). ns, p ≥ 0.05; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Gut Microbes

    Article Title: Targeted intestinal ROS scavenging alleviated rheumatoid arthritis by microbial metabolite kynurenic acid mediated Treg/Th17 balance through AhR pathway activation

    doi: 10.1080/19490976.2026.2739669

    Figure Lengend Snippet: KYNA exerted anti-rheumatic arthritis effects in vivo through the AhR pathway. (A) Detailed schematic of the animal experimental procedures of CIA PBS , CIA KYNA and CIA KYNA+CH-223191 groups. (B) Arthritis scores in CIA mice. (C) Representative photographs and micro-CT images of paws. (D) Quantitative analyses of bone histomorphometry indexes including BMD, BV/TV, Tb.Th, Tb.Sp, and Tb.N in CIA mice. (E) Representative HE staining images of joints. Scale bar, 100 μm. (F) Histological scores of the joints. (G) Representative TRAP staining images of joints. Scale bar, 100 μm. (H) Quantitative analyses of TRAP staining. (I–L) Flow cytometric analysis and quantification of IL-17A⁺ Th17 cells and FOXP3⁺ Treg cells among CD4⁺ T cells, as well as the Treg/Th17 ratio, in the spleen and MLN. (M–P) (M and O), Representative immunofluorescence staining of ZO-1 and Occludin in colon tissue. Scale bar, 100 μm. (N and P) Quantification of the MFI of ZO-1 and Occludin after normalization. (Q) Representative Western blot images of ZO-1 and Occludin in the presence of KYNA or KYNA + CH-223191 in IEC-6 cells. (R and S) Western blot quantification of ZO-1 and Occludin protein levels (normalized to β-actin). ns, p ≥ 0.05; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: For IEC-6 cells, ZO-1 (Proteintech, Cat. no. 21773-1-AP, 1:5,000) and Occludin (Proteintech, Cat. no. 27260-1-AP, 1:5,000) antibodies were used.

    Techniques: In Vivo, Micro-CT, H&E Stain, Staining, Analysis, Immunofluorescence, Tissue, Western Blot

    ABCA1 deficiency disrupts the junctional integrity of the colonic mucosal barrier. (A) Representative images of immunofluorescence staining of the zippering junction with E‐cadherin. (B, C) Effects of ABCA1 silencing on cholesterol crystals (CHO)‐induced cell injury. Caco‐2 cells were transfected with siNC or siABCA1 for 24 h, and then exposed to 1 mmol/L CHO or not for 24 h. The cell viability was determined using Calcein‐AM/PI staining (B) and CCK8 assay (C). The data in panel C are expressed as mean ± SD ( n = 4; *** p < 0.001 vs . siNC without CHO by Student's t ‐test). (D) ABCA1 silencing aggravates CHO‐mediated barrier dysfunction in Caco‐2 cells. Caco‐2 cells were transfected with siNC or siABCA1 for 24 h, and then exposed to 1 mmol/L CHO for 6 h. Cellular lysates were assayed for E‐cadherin, Occludin and ZO‐1 expression. The respective densitometric quantitations of bands are presented in the bar graphs to the right of the blots. Data are expressed as mean ± SD ( n = 3; * p < 0.05, ** p < 0.01, *** p < 0.001 vs . siNC without CHO by Student's t ‐test).

    Journal: Animal Models and Experimental Medicine

    Article Title: ABCA1 deficiency‐induced cholesterol accumulation aggravates colonic epithelial injury in knockout hamsters

    doi: 10.1002/ame2.70301

    Figure Lengend Snippet: ABCA1 deficiency disrupts the junctional integrity of the colonic mucosal barrier. (A) Representative images of immunofluorescence staining of the zippering junction with E‐cadherin. (B, C) Effects of ABCA1 silencing on cholesterol crystals (CHO)‐induced cell injury. Caco‐2 cells were transfected with siNC or siABCA1 for 24 h, and then exposed to 1 mmol/L CHO or not for 24 h. The cell viability was determined using Calcein‐AM/PI staining (B) and CCK8 assay (C). The data in panel C are expressed as mean ± SD ( n = 4; *** p < 0.001 vs . siNC without CHO by Student's t ‐test). (D) ABCA1 silencing aggravates CHO‐mediated barrier dysfunction in Caco‐2 cells. Caco‐2 cells were transfected with siNC or siABCA1 for 24 h, and then exposed to 1 mmol/L CHO for 6 h. Cellular lysates were assayed for E‐cadherin, Occludin and ZO‐1 expression. The respective densitometric quantitations of bands are presented in the bar graphs to the right of the blots. Data are expressed as mean ± SD ( n = 3; * p < 0.05, ** p < 0.01, *** p < 0.001 vs . siNC without CHO by Student's t ‐test).

    Article Snippet: Then, proteins were transferred to a polyvinylidene difluoride membrane and hybridized with the following antibodies: ABCA1 (26564‐1‐AP, Proteintech, Wuhan, China), ABCA1 (96292, Cell Signaling Technology, Danvers, MA), ZO‐1 (A0659, ABclonal, Wuhan, China), E‐cadherin (sc‐8426, Santa cruz Biotechnology, Santa Cruz, CA), Occludin (66378‐1‐Ig, Proteintech, Wuhan, China), p‐p38 (8796‐1‐AP, Proteintech, Wuhan, China) or β‐actin (66009‐1‐Ig, Proteintech, Wuhan, China).

    Techniques: Immunofluorescence, Staining, Transfection, CCK-8 Assay, Expressing