zic philic column  (Millipore)

 
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    Name:
    Glycyrrhizic acid ammonium salt from glycyrrhiza root
    Description:
    Glycyrrhizic acid GA is a non selective inhibitor of 11β hydroxysteroid dehydrogenase 11β HSD which mediates the inter conversion of active and inactive glucocorticoids GA also shows inhibitory effect on the cytokine activity of high mobility group box 1 protein HMGB1
    Catalog Number:
    G2137
    Price:
    None
    Applications:
    The product has been used a source of glycyrrhizic acid (GA), to study its effect on glucose intolerance and insulin resistance in rats fed on high-fat/high-sucrose (HF/HS) diet.
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    Structured Review

    Millipore zic philic column
    Glycyrrhizic acid ammonium salt from glycyrrhiza root
    Glycyrrhizic acid GA is a non selective inhibitor of 11β hydroxysteroid dehydrogenase 11β HSD which mediates the inter conversion of active and inactive glucocorticoids GA also shows inhibitory effect on the cytokine activity of high mobility group box 1 protein HMGB1
    https://www.bioz.com/result/zic philic column/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zic philic column - by Bioz Stars, 2021-07
    93/100 stars

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    Related Articles

    Injection:

    Article Title: Glycyrrhizin, inhibitor of high mobility group box-1, attenuates monocrotaline-induced pulmonary hypertension and vascular remodeling in rats
    Article Snippet: .. Male Sprague–Dawley rats (body weight 240–260 g) were randomly divided into 3 groups in each experiment: Group 1, initially given a single subcutaneous injection of normal saline and no treatment (control group; n = 5 [4 weeks] and 10 [6 weeks]); Group 2, initially given a single subcutaneous injection of MCT (0.5 ml, 60 mg/kg; c2401; Sigma-Aldrich) and once daily intraperitoneal treatment of normal saline (MCT group; n = 10 [4 weeks] and 20 [6 weeks]); and Group 3, initially given a single subcutaneous injection of MCT (0.5 ml, 60 mg/kg) and once daily intraperitoneal treatment of glycyrrhizin (GLY) (50 mg/kg; 50531; Sigma-Aldrich) (MCT + GLY group; n = 10 [4 weeks] and 20 [6 weeks]). ..

    Article Title: Haploinsufficiency of Def Activates p53-Dependent TGF? Signalling and Causes Scar Formation after Partial Hepatectomy
    Article Snippet: The survival rates of the wild-type and def+/− fish after surgery were greater than 95% up to 60 days after PH in all of the experiments described in this work. .. For drug treatment, 20 µM diphenyleneiodonium (D2926; Sigma) or 200 µM apocynin (W508454; Sigma) was injected into the abdominal cavity daily between days 0 and 2 after PH; 20 mg/L of Dex (D4902; Sigma) was injected immediately after PH; 100 µM of glycyrrhizic acid ammonium salt (50531; Sigma) was injected daily between days 0 and 3 after PH; and 20 µM of SB431542 (ITB1001; Gene Operation) was injected 2 days after PH. .. Histological Analysis Immunohistochemistry was performed as described previously .

    Article Title: HMGB1 promotes the activation of NLRP3 and caspase-8 inflammasomes via NF-κB pathway in acute glaucoma
    Article Snippet: After 60 min, the needle was withdrawn and tobramycin was applied to avoid bacterial infection. .. Intravitreal injection After induction of IR injury, mice received an intravitreal injection of recombinant human HMGB1 (1 μg/2 μl; 1690-HM-025, R & D system, Inc., USA), glycyrrhizic acid (120 μM/2 μl; 50531, Sigma Aldrich, USA), nuclear factor κB (NF-κB) inhibitor JSH-23 (20 μM/2 μl; J4455, Sigma Aldrich, USA) , caspase-8 inhibitor Z-IETD-fmk (20 μM/2 μl; Calbiochem, San Diego, CA, USA), and PBS (2 μl) vehicle as sham. .. Mice were sacrificed and eyes were enucleated 48 h after intravitreal injection.

    Quantitation Assay:

    Article Title: Species-specific Standardisation of Licorice by Metabolomic Profiling of Flavanones and Chalcones
    Article Snippet: The purity of each compound and the relative ratio of isomers (e.g. LigC-4- O -ApiGlc/LigC-4′- O -ApiGlc) in each sample were defined by quantitative 1 H-NMR (qHNMR) using the 100% method ( ). .. The glycyrrhizin standard (part no. 50531) used for the UHPLC/UV quantitation was purchased from Sigma-Aldrich. ..

    Transplantation Assay:

    Article Title: High Mobility Group Box 1 Contributes to the Acute Rejection of Liver Allografts by Activating Dendritic Cells
    Article Snippet: .. Assessment of the Therapeutic Effects of Glycyrrhizic Acid on Liver TransplantationTo identify whether the blockade of HMGB1 eased acute rejection-induced liver injury after transplantation, a natural inhibitor of HMGB1 named glycyrrhizic acid (Sigma, USA) was used to treat the liver graft before transplantation. ..

    other:

    Article Title: Human GAPDH Is a Target of Aspirin’s Primary Metabolite Salicylic Acid and Its Derivatives
    Article Snippet: Chemicals and anti-SA antibody Salicylic acid (SA)(Cat#: 247588), Aspirin (Cat#: A5376) and Glycyrrhizin (Cat#: 50531) were purchased from Sigma-Aldrich.

    Molecular Weight:

    Article Title: Effect of Glycyrrhizic Acid on Scopolamine-Induced Cognitive Impairment in Mice
    Article Snippet: .. Preparation of the Glycyrrhizic Acid Glycyrrhizic acid ammonium salt (C42 H62 O16 · NH3, molecular weight, 839.96) from glycyrrhiza root was obtained from Sigma-Aldrich (Sigma Chemical Co., St. Louis, MO, USA). .. AnimalsThe animals used in this study were 20-week-old male Institute of Cancer Research mice (RaonBio Inc., Seoul, Korea), and average body weight was 30±5 g. The animal room was maintained at a temperature of 22°C±2°C and a relative humidity of 40%–70%, and the room lighting was bright for 12 hours and dark for 12 hours.

    Mouse Assay:

    Article Title: HMGB1 promotes the activation of NLRP3 and caspase-8 inflammasomes via NF-κB pathway in acute glaucoma
    Article Snippet: After 60 min, the needle was withdrawn and tobramycin was applied to avoid bacterial infection. .. Intravitreal injection After induction of IR injury, mice received an intravitreal injection of recombinant human HMGB1 (1 μg/2 μl; 1690-HM-025, R & D system, Inc., USA), glycyrrhizic acid (120 μM/2 μl; 50531, Sigma Aldrich, USA), nuclear factor κB (NF-κB) inhibitor JSH-23 (20 μM/2 μl; J4455, Sigma Aldrich, USA) , caspase-8 inhibitor Z-IETD-fmk (20 μM/2 μl; Calbiochem, San Diego, CA, USA), and PBS (2 μl) vehicle as sham. .. Mice were sacrificed and eyes were enucleated 48 h after intravitreal injection.

    Recombinant:

    Article Title: HMGB1 promotes the activation of NLRP3 and caspase-8 inflammasomes via NF-κB pathway in acute glaucoma
    Article Snippet: After 60 min, the needle was withdrawn and tobramycin was applied to avoid bacterial infection. .. Intravitreal injection After induction of IR injury, mice received an intravitreal injection of recombinant human HMGB1 (1 μg/2 μl; 1690-HM-025, R & D system, Inc., USA), glycyrrhizic acid (120 μM/2 μl; 50531, Sigma Aldrich, USA), nuclear factor κB (NF-κB) inhibitor JSH-23 (20 μM/2 μl; J4455, Sigma Aldrich, USA) , caspase-8 inhibitor Z-IETD-fmk (20 μM/2 μl; Calbiochem, San Diego, CA, USA), and PBS (2 μl) vehicle as sham. .. Mice were sacrificed and eyes were enucleated 48 h after intravitreal injection.

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  • 93
    Millipore well recognized sequant zic philic columns
    Application of the redox metabolite detection method for mammalian tissues; ( a , b ) Top 25 differentially detected metabolites between three chromatographic methods in mouse liver ( a ) and kidney ( b ) presented in a heatmap for each organ. Each replicate is a tissue chunk from an independent mouse, extracted in buffer C+Ell and then split in three and run on the different chromatographies. <t>ZIC:</t> <t>ZIC-pHILIC,</t> Acc: Accucore-NH2, LUNA: LUNA-NH2; ( c ) Venn diagram of total detected metabolites in liver and kidney between the three chromatographic methods from ( a , b ) and the overlap between them. Only metabolites passing 30% CV cutoff were compared; ( d ) Global PCA analysis of polar metabolites detection in mouse liver samples extracted by three different buffers (B, C, C plus Ellman’s) and detected by LC-MS using the ZIC-pHILIC column; Each replicate represents a liver chunk from an independent mouse, subdivided evenly between three tubes and extracted using the different buffers. This experiment was performed twice and a representative replicate is shown; ( e ) Top 25 differentially detected metabolites in mouse liver samples from ( d ); ( f ) Detection of redox metabolites from mouse liver, kidney, CSF, and plasma extracted in three different buffers (B, C, and C plus Ellman’s). These experiments were performed twice (except for plasma) and a representative replicate is shown. Each dot represents a measurement from an independent mouse, mean and standard deviation are indicated; statistical significance was determined using Anova with correction for multiple comparisons by false discovery rate correction. Only significant q-values are indicated (except for GSH and GSH-Ell levels, which were excluded from analysis).
    Well Recognized Sequant Zic Philic Columns, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/well recognized sequant zic philic columns/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    well recognized sequant zic philic columns - by Bioz Stars, 2021-07
    93/100 stars
      Buy from Supplier

    93
    Millipore zic philic column
    Application of the redox metabolite detection method for mammalian tissues; ( a , b ) Top 25 differentially detected metabolites between three chromatographic methods in mouse liver ( a ) and kidney ( b ) presented in a heatmap for each organ. Each replicate is a tissue chunk from an independent mouse, extracted in buffer C+Ell and then split in three and run on the different chromatographies. <t>ZIC:</t> <t>ZIC-pHILIC,</t> Acc: Accucore-NH2, LUNA: LUNA-NH2; ( c ) Venn diagram of total detected metabolites in liver and kidney between the three chromatographic methods from ( a , b ) and the overlap between them. Only metabolites passing 30% CV cutoff were compared; ( d ) Global PCA analysis of polar metabolites detection in mouse liver samples extracted by three different buffers (B, C, C plus Ellman’s) and detected by LC-MS using the ZIC-pHILIC column; Each replicate represents a liver chunk from an independent mouse, subdivided evenly between three tubes and extracted using the different buffers. This experiment was performed twice and a representative replicate is shown; ( e ) Top 25 differentially detected metabolites in mouse liver samples from ( d ); ( f ) Detection of redox metabolites from mouse liver, kidney, CSF, and plasma extracted in three different buffers (B, C, and C plus Ellman’s). These experiments were performed twice (except for plasma) and a representative replicate is shown. Each dot represents a measurement from an independent mouse, mean and standard deviation are indicated; statistical significance was determined using Anova with correction for multiple comparisons by false discovery rate correction. Only significant q-values are indicated (except for GSH and GSH-Ell levels, which were excluded from analysis).
    Zic Philic Column, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic column/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zic philic column - by Bioz Stars, 2021-07
    93/100 stars
      Buy from Supplier

    94
    Millipore sequant zic philic column
    Application of the redox metabolite detection method for mammalian tissues; ( a , b ) Top 25 differentially detected metabolites between three chromatographic methods in mouse liver ( a ) and kidney ( b ) presented in a heatmap for each organ. Each replicate is a tissue chunk from an independent mouse, extracted in buffer C+Ell and then split in three and run on the different chromatographies. <t>ZIC:</t> <t>ZIC-pHILIC,</t> Acc: Accucore-NH2, LUNA: LUNA-NH2; ( c ) Venn diagram of total detected metabolites in liver and kidney between the three chromatographic methods from ( a , b ) and the overlap between them. Only metabolites passing 30% CV cutoff were compared; ( d ) Global PCA analysis of polar metabolites detection in mouse liver samples extracted by three different buffers (B, C, C plus Ellman’s) and detected by LC-MS using the ZIC-pHILIC column; Each replicate represents a liver chunk from an independent mouse, subdivided evenly between three tubes and extracted using the different buffers. This experiment was performed twice and a representative replicate is shown; ( e ) Top 25 differentially detected metabolites in mouse liver samples from ( d ); ( f ) Detection of redox metabolites from mouse liver, kidney, CSF, and plasma extracted in three different buffers (B, C, and C plus Ellman’s). These experiments were performed twice (except for plasma) and a representative replicate is shown. Each dot represents a measurement from an independent mouse, mean and standard deviation are indicated; statistical significance was determined using Anova with correction for multiple comparisons by false discovery rate correction. Only significant q-values are indicated (except for GSH and GSH-Ell levels, which were excluded from analysis).
    Sequant Zic Philic Column, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic philic column/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sequant zic philic column - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

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    Application of the redox metabolite detection method for mammalian tissues; ( a , b ) Top 25 differentially detected metabolites between three chromatographic methods in mouse liver ( a ) and kidney ( b ) presented in a heatmap for each organ. Each replicate is a tissue chunk from an independent mouse, extracted in buffer C+Ell and then split in three and run on the different chromatographies. ZIC: ZIC-pHILIC, Acc: Accucore-NH2, LUNA: LUNA-NH2; ( c ) Venn diagram of total detected metabolites in liver and kidney between the three chromatographic methods from ( a , b ) and the overlap between them. Only metabolites passing 30% CV cutoff were compared; ( d ) Global PCA analysis of polar metabolites detection in mouse liver samples extracted by three different buffers (B, C, C plus Ellman’s) and detected by LC-MS using the ZIC-pHILIC column; Each replicate represents a liver chunk from an independent mouse, subdivided evenly between three tubes and extracted using the different buffers. This experiment was performed twice and a representative replicate is shown; ( e ) Top 25 differentially detected metabolites in mouse liver samples from ( d ); ( f ) Detection of redox metabolites from mouse liver, kidney, CSF, and plasma extracted in three different buffers (B, C, and C plus Ellman’s). These experiments were performed twice (except for plasma) and a representative replicate is shown. Each dot represents a measurement from an independent mouse, mean and standard deviation are indicated; statistical significance was determined using Anova with correction for multiple comparisons by false discovery rate correction. Only significant q-values are indicated (except for GSH and GSH-Ell levels, which were excluded from analysis).

    Journal: Metabolites

    Article Title: Redox Metabolism Measurement in Mammalian Cells and Tissues by LC-MS

    doi: 10.3390/metabo11050313

    Figure Lengend Snippet: Application of the redox metabolite detection method for mammalian tissues; ( a , b ) Top 25 differentially detected metabolites between three chromatographic methods in mouse liver ( a ) and kidney ( b ) presented in a heatmap for each organ. Each replicate is a tissue chunk from an independent mouse, extracted in buffer C+Ell and then split in three and run on the different chromatographies. ZIC: ZIC-pHILIC, Acc: Accucore-NH2, LUNA: LUNA-NH2; ( c ) Venn diagram of total detected metabolites in liver and kidney between the three chromatographic methods from ( a , b ) and the overlap between them. Only metabolites passing 30% CV cutoff were compared; ( d ) Global PCA analysis of polar metabolites detection in mouse liver samples extracted by three different buffers (B, C, C plus Ellman’s) and detected by LC-MS using the ZIC-pHILIC column; Each replicate represents a liver chunk from an independent mouse, subdivided evenly between three tubes and extracted using the different buffers. This experiment was performed twice and a representative replicate is shown; ( e ) Top 25 differentially detected metabolites in mouse liver samples from ( d ); ( f ) Detection of redox metabolites from mouse liver, kidney, CSF, and plasma extracted in three different buffers (B, C, and C plus Ellman’s). These experiments were performed twice (except for plasma) and a representative replicate is shown. Each dot represents a measurement from an independent mouse, mean and standard deviation are indicated; statistical significance was determined using Anova with correction for multiple comparisons by false discovery rate correction. Only significant q-values are indicated (except for GSH and GSH-Ell levels, which were excluded from analysis).

    Article Snippet: In addition to the well-recognized SeQuant ZIC-pHILIC columns (Millipore-Sigma, St. Louis, MI, USA) we decided to explore two more consistently available columns: Accucore-Amide HILIC (Thermo-Fisher Scientific, Waltham, MA, USA) and LUNA-NH2 (Phenomenex, Torrance, CA, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Standard Deviation

    A method to quantify redox metabolites by LC-MS; ( a,b ) Retention times and sensitivity comparison between three HILIC-based chromatography methods. Chromatographic runs were carried out on either ZIC-pHILIC (ZIC), Accucore-NH2 (ACC), or LUNA-NH2 (LUNA) with 20 min, 20 min, or 25 min linear gradients, respectively. Overlaid peaks are shown for the indicated range of ( a ) polar metabolite standards (see Table S1 for further details) and ( b ) GSH, GSH derivatized with Ellman’s (GSH-Ell), GSSG, NADH, and NADPH. Redox metabolites were diluted in 25 mM Ammonium Acetate and 2.5 mM Na-Ascorbate in water. Standards were dissolved in extraction buffer B (as detailed in Section 2.2 ); ( c ) Optimization of HESI parameters on orbitrap mass spectrometer for the indicated metabolites using ZIC-pHILIC chromatography and standards. Graphs represent integrated areas of chromatographic peaks under changing parameters for capillary temperature or S-lens; ( d ) Limit of detection and linearity for individual redox molecules. Presented are the average values and standard deviation.

    Journal: Metabolites

    Article Title: Redox Metabolism Measurement in Mammalian Cells and Tissues by LC-MS

    doi: 10.3390/metabo11050313

    Figure Lengend Snippet: A method to quantify redox metabolites by LC-MS; ( a,b ) Retention times and sensitivity comparison between three HILIC-based chromatography methods. Chromatographic runs were carried out on either ZIC-pHILIC (ZIC), Accucore-NH2 (ACC), or LUNA-NH2 (LUNA) with 20 min, 20 min, or 25 min linear gradients, respectively. Overlaid peaks are shown for the indicated range of ( a ) polar metabolite standards (see Table S1 for further details) and ( b ) GSH, GSH derivatized with Ellman’s (GSH-Ell), GSSG, NADH, and NADPH. Redox metabolites were diluted in 25 mM Ammonium Acetate and 2.5 mM Na-Ascorbate in water. Standards were dissolved in extraction buffer B (as detailed in Section 2.2 ); ( c ) Optimization of HESI parameters on orbitrap mass spectrometer for the indicated metabolites using ZIC-pHILIC chromatography and standards. Graphs represent integrated areas of chromatographic peaks under changing parameters for capillary temperature or S-lens; ( d ) Limit of detection and linearity for individual redox molecules. Presented are the average values and standard deviation.

    Article Snippet: In addition to the well-recognized SeQuant ZIC-pHILIC columns (Millipore-Sigma, St. Louis, MI, USA) we decided to explore two more consistently available columns: Accucore-Amide HILIC (Thermo-Fisher Scientific, Waltham, MA, USA) and LUNA-NH2 (Phenomenex, Torrance, CA, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Hydrophilic Interaction Liquid Chromatography, Chromatography, Mass Spectrometry, Standard Deviation