zic philic column  (Millipore)


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    Structured Review

    Millipore zic philic column
    Zic Philic Column, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic column/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    zic philic column - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Electron Microscopy:

    Article Title: Dirofilaria immitis Microfilariae and Third-Stage Larvae Induce Canine NETosis Resulting in Different Types of Neutrophil Extracellular Traps
    Article Snippet: .. Scanning Electron Microscopy (SEM) Canine PMN (n = 3.5 × 105 ) were cocultured with vital D. immitis L3 (10 larvae/sample) or microfilariae (20 larvae/sample) on poly-l -lysine (Sigma-Aldrich) pre-coated coverslips (60 min, 37°C). .. After incubation, the samples were fixed in 2.5% glutaraldehyde [60 min, room temperature (RT), Merck], post-fixed in 1% osmium tetroxide (Merck), washed in distilled water, dehydrated, critical point dried by CO2 treatment, and spayed with gold.

    Whole Genome Amplification:

    Article Title: Genome wide identification of promoter binding sites for H4K12ac in human sperm and its relevance for early embryonic development
    Article Snippet: .. The required amount of DNA for microarray analysis (4 µg per sample) was generated using WGA Re-amplification Kit (Sigma-Aldrich, Steinheim, Germany). ..

    Buffer Exchange:

    Article Title: An accurate TMT-based approach to quantify and model lysine susceptibility to conjugation via N-hydroxysuccinimide esters in a monoclonal antibody
    Article Snippet: .. For the deglycosylation experiment, 400 μg of NIST mAb was diluted to 5 mg/mL through the addition of 45 μL of PBS (control glycosylated sample) or PNGaseF (Sigma, deglycosylated sample) and incubated at 37 °C for 5 h, prior to the initial buffer exchange step. .. To remove sample heterogeneity due to glycans and C-terminal lysine residues prior to intact mass analysis, a 20 μg aliquot of each TMT-labeled sample was treated with 4 μL PNGaseF overnight at 37 °C followed by a 2 h incubation with 4 μL of Carboxypeptidase B (Sigma, 0.05 mg/mL).

    Incubation:

    Article Title: An accurate TMT-based approach to quantify and model lysine susceptibility to conjugation via N-hydroxysuccinimide esters in a monoclonal antibody
    Article Snippet: .. For the deglycosylation experiment, 400 μg of NIST mAb was diluted to 5 mg/mL through the addition of 45 μL of PBS (control glycosylated sample) or PNGaseF (Sigma, deglycosylated sample) and incubated at 37 °C for 5 h, prior to the initial buffer exchange step. .. To remove sample heterogeneity due to glycans and C-terminal lysine residues prior to intact mass analysis, a 20 μg aliquot of each TMT-labeled sample was treated with 4 μL PNGaseF overnight at 37 °C followed by a 2 h incubation with 4 μL of Carboxypeptidase B (Sigma, 0.05 mg/mL).

    Purification:

    Article Title: Molecular Determinants of Scaffold-induced Linear Ubiquitinylation of B Cell Lymphoma/Leukemia 10 (Bcl10) during T Cell Receptor and Oncogenic Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling *
    Article Snippet: .. Purified mouse CD4+ T cells (7 × 107 /sample) or Jurkat T cells (1 × 108 /sample) were stimulated with or without 50 ng/ml PMA (Sigma) and 1 μ m iono (Sigma) for 30 min at 37 °C. ..

    Microarray:

    Article Title: Genome wide identification of promoter binding sites for H4K12ac in human sperm and its relevance for early embryonic development
    Article Snippet: .. The required amount of DNA for microarray analysis (4 µg per sample) was generated using WGA Re-amplification Kit (Sigma-Aldrich, Steinheim, Germany). ..

    Generated:

    Article Title: Genome wide identification of promoter binding sites for H4K12ac in human sperm and its relevance for early embryonic development
    Article Snippet: .. The required amount of DNA for microarray analysis (4 µg per sample) was generated using WGA Re-amplification Kit (Sigma-Aldrich, Steinheim, Germany). ..

    Western Blot:

    Article Title: Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells
    Article Snippet: .. Western immunoblotting Cell lysates (20.0 μg of protein/sample) were first resolved on a 12.5% SDS-PAGE gel, transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore Corporation, Billerica, MA) and then blocked using Tris buffered saline Tween-20 (TBST) buffer containing 5% non-fat milk. .. Membranes were next hybridized overnight in the cold room using various murine monoclonal antibodies (mAb) – i.e. S10B1 anti-Ku86 N-terminus (amino acid (aa) residues 8–221; NeoMarkers, Fremont, CA); anti-heavy chain of the human major histocompatibility complex (MHC) mAb (clone 22.63.4, Accurate Chemical and Scientific Co., Westbuty, NY; a kind gift from P. Macary, from the National University of Singapore); and anti-actin (Santa Cruz Biotechnology, Santa Cruz, CA) mAbs; washed three times in ice cold TBS-T; and then incubated with horseradish peroxidase (hrp) conjugated anti-mouse IgG mAb (1:2,000; Santa Cruz) for 1 hr.

    SDS Page:

    Article Title: Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells
    Article Snippet: .. Western immunoblotting Cell lysates (20.0 μg of protein/sample) were first resolved on a 12.5% SDS-PAGE gel, transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore Corporation, Billerica, MA) and then blocked using Tris buffered saline Tween-20 (TBST) buffer containing 5% non-fat milk. .. Membranes were next hybridized overnight in the cold room using various murine monoclonal antibodies (mAb) – i.e. S10B1 anti-Ku86 N-terminus (amino acid (aa) residues 8–221; NeoMarkers, Fremont, CA); anti-heavy chain of the human major histocompatibility complex (MHC) mAb (clone 22.63.4, Accurate Chemical and Scientific Co., Westbuty, NY; a kind gift from P. Macary, from the National University of Singapore); and anti-actin (Santa Cruz Biotechnology, Santa Cruz, CA) mAbs; washed three times in ice cold TBS-T; and then incubated with horseradish peroxidase (hrp) conjugated anti-mouse IgG mAb (1:2,000; Santa Cruz) for 1 hr.

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  • 90
    Millipore sequant zic philic column
    Sequant Zic Philic Column, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic philic column/product/Millipore
    Average 90 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    sequant zic philic column - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    90
    Millipore sequant zic philic polymeric column
    Sequant Zic Philic Polymeric Column, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic philic polymeric column/product/Millipore
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    sequant zic philic polymeric column - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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