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Merck KGaA zic philic column
Zic Philic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zic philic column/product/Merck KGaA
Average 92 stars, based on 32 article reviews
Price from $9.99 to $1999.99
zic philic column - by Bioz Stars, 2020-09
92/100 stars

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High Performance Liquid Chromatography:

Article Title: Suppression of autophagy impedes glioblastoma development and induces senescence
Article Snippet: .. Extracted metabolites were separated on a Zic-pHILIC column (Merck Millipore) using a Thermo Ultimate BioRS HPLC with a single step linear gradient of 1095%– A over 20 min (mobile phases were [A] 20 mM ammonium carbonate [B] acetonitrile). .. Metabolites were eluted into a Q-Exactive mass spectrometer (Thermo Fisher Scientific) using a flow rate of 300 µl/min.

Article Title: Oncogenic Kras induces Nix-mediated mitophagy to promote pancreatic cancer
Article Snippet: .. The HPLC setup consisted of a ZIC-pHILIC column (SeQuant, 150 × 2.1 mm, 5 μm, Merck KGaA, Darmstadt, Germany), with a ZIC-pHILIC guard column (SeQuant, 20 × 2.1 mm) and an initial mobile phase of 20% 20 mM ammonium carbonate, pH 9.4, and 80% acetonitrile. .. Cell and medium extracts (5 μl) were injected and metabolites were separated over a 15-minute mobile phase gradient, decreasing the acetonitrile content to 20%, at a flow rate of 200 μL/min and a column temperature of 45°C.

Article Title: Metabolomic Analyses of Leishmania Reveal Multiple Species Differences and Large Differences in Amino Acid Metabolism
Article Snippet: .. 10 μl of samples was injected onto a ZIC-pHILIC column (150 mm×4.6 mm; 3.5 μm, Merck, Germany) with mobile phase A being 20 mM ammonium carbonate in HPLC grade water (pH 9.2) and mobile phase B being HPLC grade acetonitrile. ..

Article Title: Improving the metabolic fidelity of cancer models with a physiological cell culture medium
Article Snippet: .. The HPLC setup consisted of a ZIC-pHILIC column (SeQuant; 150 mm by 2.1 mm, 5 μm; Merck KGaA, Darmstadt, Germany), with a ZIC-pHILIC guard column (SeQuant; 20 mm by 2.1 mm). .. Biological extracts were injected (5 μl), and compounds were separated with a mobile phase gradient of 15 min, which started with 20% ammonium carbonate [20 mM (pH 9.2)] and 80% acetonitrile, and terminated with 20% acetonitrile.

Injection:

Article Title: Metabolomic Analyses of Leishmania Reveal Multiple Species Differences and Large Differences in Amino Acid Metabolism
Article Snippet: .. 10 μl of samples was injected onto a ZIC-pHILIC column (150 mm×4.6 mm; 3.5 μm, Merck, Germany) with mobile phase A being 20 mM ammonium carbonate in HPLC grade water (pH 9.2) and mobile phase B being HPLC grade acetonitrile. ..

Flow Cytometry:

Article Title: Gastroparesis and lipid metabolism-associated dysbiosis in Wistar-Kyoto rats
Article Snippet: .. Compounds were separated using a 5 µm ZIC-pHILIC column (100 × 2.1 mm; Merck, Darmstadt, Germany) eluted with solvent A : acetonitrile with 0.1% formic acid; and solvent B : 16 mM ammonium formate in water, at a flow rate of 250 µl/min. ..

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    Merck KGaA sequant zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic philic column/product/Merck KGaA
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sequant zic philic column - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    92
    Merck KGaA zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Zic Philic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic column/product/Merck KGaA
    Average 92 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    zic philic column - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a SeQuant ZIC-pHILIC column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.

    Journal: Frontiers in Microbiology

    Article Title: Identification and Functional Characterization of Small Alarmone Synthetases in Corynebacterium glutamicum

    doi: 10.3389/fmicb.2017.01601

    Figure Lengend Snippet: In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a SeQuant ZIC-pHILIC column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.

    Article Snippet: HPLC analysis of all assay reactions was performed using a LaChrome ULTRA system (HITACHI) and a SeQuant ZIC-pHILIC column (Merck Millipore).

    Techniques: In Vitro, Activity Assay, High Performance Liquid Chromatography, Concentration Assay, Mass Spectrometry