vps34 antibody (Echelon Biosciences)


Structured Review

Vps34 Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vps34 antibody/product/Echelon Biosciences
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling"
Article Title: Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling
Journal: Nature Communications
doi: 10.1038/ncomms9283

Figure Legend Snippet: UVRAG-associated class III lipid kinase is activated by insulin. ( a ) ATG14- and UVRAG-containing Vps34 complexes were immunoprecipitated from Hepa1.6 cells grown in the presence (NR) or absence of amino acids (−AA) and assayed for lipid kinase activity. Inputs for each assay were immunoblotted to determine the amounts of the Vps34 co-immunoprecipitated. PI3P signals were densitometrically measured and normalized to Vps34 protein levels. The data are presented as a fold difference of PI3P normalized to co-immunoprecipitated Vps34 levels revealed by immunoblot for each condition. Data are means±s.e.m. ( n =4, * P
Techniques Used: Immunoprecipitation, Activity Assay
2) Product Images from "Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy"
Article Title: Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy
Journal: Proceedings of the National Academy of Sciences of the United States of America
doi: 10.1073/pnas.1217692110

Figure Legend Snippet: Hsp70 regulates Beclin-1–Vps34 interaction and autophagosome formation. ( A ) Identification of proteins in a PS-induced Vps34 complex. Vps34 immunoprecipitates from PS-treated MCF7 cells were separated by SDS/PAGE and silver stained. Distinct bands
Techniques Used: SDS Page, Staining

Figure Legend Snippet: KAP1 is the SUMO E3 ligase for Vps34. ( A ) PS induces cytoplasmic accumulation of KAP1. MCF7 cells were treated with PS for 24 h, as indicated. ( Upper ) Immunoblot analyses of KAP1 and β-actin in cytosolic extracts. ( Lower ) Immunostaining of KAP1
Techniques Used: Immunostaining

Figure Legend Snippet: Role of hsp70 in autophagosome formation in breast cancer cells. ( A and B ) Amino acid-starvation induces hsp70 acetylation, Vps34 SUMOylation and enhances KAP1 binding to Vps34 and acetylated hsp70. Immunoblot analyses of Vps34 ( A ) and KAP1 ( B ) immunoprecipitates
Techniques Used: Binding Assay