anti vps34 antibody (Echelon Biosciences)


Structured Review

Anti Vps34 Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti vps34 antibody/product/Echelon Biosciences
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Resistance to Inhibitors of Cholinesterase (Ric)-8A and Gα i Contribute to Cytokinesis Abscission by Controlling Vacuolar Protein-Sorting (Vps)34 Activity"
Article Title: Resistance to Inhibitors of Cholinesterase (Ric)-8A and Gα i Contribute to Cytokinesis Abscission by Controlling Vacuolar Protein-Sorting (Vps)34 Activity
Journal: PLoS ONE
doi: 10.1371/journal.pone.0086680

Figure Legend Snippet: ( A ) Vps34, GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).
Techniques Used: Western Blot, Transfection, Expressing, Immunoprecipitation, Purification
anti vps34 antibody (Echelon Biosciences)


Structured Review

Anti Vps34 Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti vps34 antibody/product/Echelon Biosciences
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Resistance to Inhibitors of Cholinesterase (Ric)-8A and Gα i Contribute to Cytokinesis Abscission by Controlling Vacuolar Protein-Sorting (Vps)34 Activity"
Article Title: Resistance to Inhibitors of Cholinesterase (Ric)-8A and Gα i Contribute to Cytokinesis Abscission by Controlling Vacuolar Protein-Sorting (Vps)34 Activity
Journal: PLoS ONE
doi: 10.1371/journal.pone.0086680

Figure Legend Snippet: ( A ) Vps34, GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).
Techniques Used: Western Blot, Transfection, Expressing, Immunoprecipitation, Purification
anti vps34 (Echelon Biosciences)


Structured Review

Anti Vps34, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti vps34/product/Echelon Biosciences
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Resistance to Inhibitors of Cholinesterase (Ric)-8A and Gα i Contribute to Cytokinesis Abscission by Controlling Vacuolar Protein-Sorting (Vps)34 Activity"
Article Title: Resistance to Inhibitors of Cholinesterase (Ric)-8A and Gα i Contribute to Cytokinesis Abscission by Controlling Vacuolar Protein-Sorting (Vps)34 Activity
Journal: PLoS ONE
doi: 10.1371/journal.pone.0086680

Figure Legend Snippet: ( A ) Vps34, GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).
Techniques Used: Western Blot, Transfection, Expressing, Immunoprecipitation, Purification
anti vps34 (Echelon Biosciences)


Structured Review

Anti Vps34, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti vps34/product/Echelon Biosciences
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Resistance to Inhibitors of Cholinesterase (Ric)-8A and Gα i Contribute to Cytokinesis Abscission by Controlling Vacuolar Protein-Sorting (Vps)34 Activity"
Article Title: Resistance to Inhibitors of Cholinesterase (Ric)-8A and Gα i Contribute to Cytokinesis Abscission by Controlling Vacuolar Protein-Sorting (Vps)34 Activity
Journal: PLoS ONE
doi: 10.1371/journal.pone.0086680

Figure Legend Snippet: ( A ) Vps34, GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).
Techniques Used: Western Blot, Transfection, Expressing, Immunoprecipitation, Purification
anti hvps34 antibody (Echelon Biosciences)


Structured Review
Anti Hvps34 Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti hvps34 antibody/product/Echelon Biosciences
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
vps34 (Echelon Biosciences)


Structured Review

Vps34, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vps34/product/Echelon Biosciences
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma"
Article Title: NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma
Journal: Experimental & Molecular Medicine
doi: 10.1038/s12276-022-00873-2

Figure Legend Snippet: a Autophagy-related protein levels after treatment confirmed by western blot. b Schematic images of the autophagy initiation step involved in NRBF2. c Coimmunoprecipitation of NRBF2 and the VPS34 complex confirmed by western blot in GBM cell lines. d Autophagic flux analysis using the mCherry-EGFP reporter. A172 and U-87 MG cells transfected with mCherry-GFP-LC3B were treated with IR (3 Gy), siNRBF2 or both. The mean numbers of autophagosomes and autolysosomes are represented by yellow and red dots, respectively, in the merged images. The images were quantified using ImageJ ( n = 5). Scale bars, 10 µm. e IHC of LC3B in coronal sections from U-87 WT or NRBF2 knockout orthotopic xenograft mouse models treated with radiation (2 Gy × 5). Scale bars, 10 μm. ** p < 0.01, *** p < 0.001, **** p < 0.0001 using an unpaired t test.
Techniques Used: Western Blot, Transfection, Knock-Out
anti vps34 antibody (Echelon Biosciences)


Structured Review

Anti Vps34 Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti vps34 antibody/product/Echelon Biosciences
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding"
Article Title: The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding
Journal: Cell reports
doi: 10.1016/j.celrep.2021.109184

Figure Legend Snippet: (A) Immunofluorescence staining of adiponectin (green) and Becn1 (red) in SVF primary adipocytes isolated from WT or Becn1 F121A mice. The intensity line profile measurements show intensity distribution on dashed arrows in the images above. Pearson correlation coefficient is used to quantify colocalization between adiponectin and Becn1. Low magnification, scale bars: 10 μm; high magnification, scale bars: 5 μm. n = 7 cells. t test. (B) The exocyst component Sec6, but not Exo84 or Exo70, is pulled down by Becn1 F121A more strongly than WT Becn1. CoIP of FLAG-Sec6 by HA-Becn1 in HEK293 cells transfected with Sec6 and WT Becn1 or Becn1 F121A . (C) Becn1 F121A promotes exocyst assembly. CoIP of HA-Becn1, Sec8, and Sec5 by FLAG-Sec6 in HEK293 cells transfected with Sec6 and WT Becn1 or Becn1 F121A . (D) CoIP of endogenous exocyst components (Sec6, Sec5, and Sec8) and autophagy-related PtdIns3K proteins (Atg14, Vps34, and UVRAG) with the endogenous Becn1 protein in primary adipocytes isolated from WT or Becn1 F121A mice. One-way ANOVA with Tukey-Kramer test. (E) CoIP of HA-hSec6 (human Sec6) and indicated full-length (FL) and deletion mutants of FLAG-hBecn1 F123A (human Becn1 F123A , mouse Becn1 F121A equivalent) in HEK293 cells. (F) WB analysis of adiponectin levels in the conditioned medium secreted from WT 3T3-L1-differentiated adipocytes stably expressing HA-tagged FL Becn1 F121A or the Becn1 F121A ΔCCD deletion mutant after 24-h culture. Intracellular adiponectin and total protein loading (Ponceau S staining) are also shown. n = 3. (G) ELISA of adiponectin and leptin in the conditioned medium of WT 3T3-L1-differentiated adipocytes stably expressing HA-tagged FL Becn1 F121A or the Becn1 F121A ΔCCD deletion mutant after 24-h culture. n = 6. (H) Leptin and adiponectin vesicle pools have limited colocalization in adipocytes. Immunofluorescence staining of adiponectin and leptin in primary adipocytes of WT mice. Low magnification, scale bars: 10 μm; high magnification, scale bars: 5 μm. n = 12 regions. Data represent mean ± SEM. One-way ANOVA with Tukey-Kramer test. *p < 0.05; **p < 0.01.
Techniques Used: Immunofluorescence, Staining, Isolation, Transfection, Stable Transfection, Expressing, Mutagenesis, Enzyme-linked Immunosorbent Assay

Figure Legend Snippet:
Techniques Used: shRNA, Recombinant, Transfection, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Clone Assay, Bicinchoninic Acid Protein Assay, Knock-In, Plasmid Preparation, Software
antibodies against pik3c3 z r016 (Echelon Biosciences)


Structured Review

Antibodies Against Pik3c3 Z R016, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against pik3c3 z r016/product/Echelon Biosciences
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy"
Article Title: The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy
Journal: American Journal of Physiology - Renal Physiology
doi: 10.1152/ajprenal.00381.2019

Figure Legend Snippet: Generation of a Pik3c3 hypomorphic mouse model. Schematic illustration of the wild-type allele, targeting vector, and hypomorphic allele of the mouse Pik3c3 gene, which enabled us to generate homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice and heterozygous Pik3c3-hypomorphic (Pik3c3Hypo/WT) mice to compare with their wild-type (Pik3c3WT/WT) littermates (A). Both immunoblot (B) and immunohistochemistry staining (C) indicated that Pik3c3Hypo/Hypo mice express a markedly lower level of Pik3c3 than Pik3c3Hypo/WT littermates, which already express a lower level of Pik3c3 than Pik3c3WT/WT littermates. E, embroynic day. Shown are representative blots and images from n = 6–8 mice/genotype group with similar results.
Techniques Used: Plasmid Preparation, Western Blot, Immunohistochemistry, Staining

Figure Legend Snippet: The reduction of Pik3c3 expression also occurs in other organs of the Pik3c3 hypomorphic mouse model. Immunoblot analysis indicated that homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice (Homo) express a markedly lower level of Pik3c3 than heterozygous Pik3c3 hypomorphic (Pik3c3Hypo/WT) littermates (Het), which already express a lower level of Pik3c3 than wild-type (Pik3c3WT/WT) littermates (WT). Of note, although an equal amount (10 or 20 μg) of total protein from different tissue homogenates was loaded, both β-actin and α-tubulin (two commonly used loading controls) were expressed at considerably different levels in different tissues, reminiscent to a previous report (28). However, expression levels of either β-actin or α-tubulin in the same type of tissues examined (including spleen, brain, lung, heart, liver, pancreas, testis, and adipose tissue) were comparable among WT, Het, and Homo Pik3c3 hypomorphic mice, validating the comparison of Pik3c3 expression levels as well as the equal loading of total protein. Shown are representative blots from n = 6 mice/genotype group with similar results.
Techniques Used: Expressing, Western Blot

Figure Legend Snippet: The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice did not cause any apparent phenotype. Pik3c3Hypo/Hypo mice had a mean body weight (A), left kidney weight (B), right kidney weight (C), and left and right kidney-to-body weight ratio (D) that were not statistically different from those of Pik3c3Hypo/WT and Pik3c3WT/WT littermates. Values are means ± SE; n = 7 mice for each group. Furthermore, hematoxylin and eosin staining revealed no difference in renal histology among Pik3c3Hypo/Hypo, Pik3c3Hypo/WT, and Pik3c3WT/WT mice (E). Shown are representative images from n = 6 for each group with similar results.
Techniques Used: Expressing, Staining

Figure Legend Snippet: Body weight, left kidney weight, left kidney-to-body weight ratio, BUN, and serum creatinine levels in Pik3c3 WT/WT , Pik3c3 Hypo/WT , and Pik3c3 Hypo/Hypo mice 7 days after sham or uninephrectomy surgery
Techniques Used:

Figure Legend Snippet: The enlargement of proximal tubules and glomeruli induced by unilateral nephrectomy (UNX) was inhibited in Pik3c3 hypomorphic (Hypo) mice. UNX-induced renal glomerular hypertrophy and proximal tubular hypertrophy were assessed by double immunofluorescence staining with synaptopodin to highlight glomeruli in red and Lotus tetragonolobus lectin (LTL) to label renal proximal tubules in green (A). The area of proximal tubules (B) and area of glomeruli (C) were measured as detailed in methods (n = 7 mice/group). Values are means ± SE. P values for the compared groups are indicated. N.S., not statistically significant; WT, wild type.
Techniques Used: Double Immunofluorescence Staining

Figure Legend Snippet: The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice inhibits the mammalian target of rapamycin complex 1 signaling pathway. Immunoblot analysis of renal cortical homogenates revealed a graded reduction of unilateral nephrectomy (UNX)-induced ribosomal protein S6 (rpS6) phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (A). Each lane represents one sample from the left kidney of a right uninephrectomized or sham-operated individual mouse. Immunohistochemistry (IHC) staining confirmed the graded inhibition of UNX-induced rpS6 phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (B). Shown are representative blots and images from n = 7 mice/group with similar results.
Techniques Used: Expressing, Western Blot, Immunohistochemistry, Inhibition

Figure Legend Snippet: A schematic summary illustrating the main concept of the present work demonstrating that Pik3c3 plays a pivotal role in mediating nephron deficit-induced compensatory nephron hypertrophy (CNH). Specifically, using a mouse model of uninephrectomy (UNX) to induce a 50% nephron reduction, the present study provides the first definitive evidence showing that Pikc3c plays an indispensable role in regulating the degree of CNH by generating Pik3c3 hypomorphic mouse model. Our hypertrophy data indicate that the degree of UNX-induced CNH is tightly regulated by the expression level of Pik3c3. Together with our previous demonstration that phosphorylated ribosomal protein S6 (rpS6) is a downstream effector of the mammalian target of rapamycin complex 1 (mTORC1)-S6 kinase 1 (S6K1) signaling pathway mediating CNH (53), our signaling data examining the level of rpS6 phosphorylation in the present study indicate that Pik3c3 functions upstream of the mTORC1-S6K1-rpS6 pathway mediating increased protein synthesis to drive the development of CNH.
Techniques Used: Expressing
antibodies against pik3c3 z r016 (Echelon Biosciences)


Structured Review

Antibodies Against Pik3c3 Z R016, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against pik3c3 z r016/product/Echelon Biosciences
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy"
Article Title: The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy
Journal: American Journal of Physiology - Renal Physiology
doi: 10.1152/ajprenal.00381.2019

Figure Legend Snippet: Generation of a Pik3c3 hypomorphic mouse model. Schematic illustration of the wild-type allele, targeting vector, and hypomorphic allele of the mouse Pik3c3 gene, which enabled us to generate homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice and heterozygous Pik3c3-hypomorphic (Pik3c3Hypo/WT) mice to compare with their wild-type (Pik3c3WT/WT) littermates (A). Both immunoblot (B) and immunohistochemistry staining (C) indicated that Pik3c3Hypo/Hypo mice express a markedly lower level of Pik3c3 than Pik3c3Hypo/WT littermates, which already express a lower level of Pik3c3 than Pik3c3WT/WT littermates. E, embroynic day. Shown are representative blots and images from n = 6–8 mice/genotype group with similar results.
Techniques Used: Plasmid Preparation, Western Blot, Immunohistochemistry, Staining

Figure Legend Snippet: The reduction of Pik3c3 expression also occurs in other organs of the Pik3c3 hypomorphic mouse model. Immunoblot analysis indicated that homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice (Homo) express a markedly lower level of Pik3c3 than heterozygous Pik3c3 hypomorphic (Pik3c3Hypo/WT) littermates (Het), which already express a lower level of Pik3c3 than wild-type (Pik3c3WT/WT) littermates (WT). Of note, although an equal amount (10 or 20 μg) of total protein from different tissue homogenates was loaded, both β-actin and α-tubulin (two commonly used loading controls) were expressed at considerably different levels in different tissues, reminiscent to a previous report (28). However, expression levels of either β-actin or α-tubulin in the same type of tissues examined (including spleen, brain, lung, heart, liver, pancreas, testis, and adipose tissue) were comparable among WT, Het, and Homo Pik3c3 hypomorphic mice, validating the comparison of Pik3c3 expression levels as well as the equal loading of total protein. Shown are representative blots from n = 6 mice/genotype group with similar results.
Techniques Used: Expressing, Western Blot

Figure Legend Snippet: The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice did not cause any apparent phenotype. Pik3c3Hypo/Hypo mice had a mean body weight (A), left kidney weight (B), right kidney weight (C), and left and right kidney-to-body weight ratio (D) that were not statistically different from those of Pik3c3Hypo/WT and Pik3c3WT/WT littermates. Values are means ± SE; n = 7 mice for each group. Furthermore, hematoxylin and eosin staining revealed no difference in renal histology among Pik3c3Hypo/Hypo, Pik3c3Hypo/WT, and Pik3c3WT/WT mice (E). Shown are representative images from n = 6 for each group with similar results.
Techniques Used: Expressing, Staining

Figure Legend Snippet: Body weight, left kidney weight, left kidney-to-body weight ratio, BUN, and serum creatinine levels in Pik3c3 WT/WT , Pik3c3 Hypo/WT , and Pik3c3 Hypo/Hypo mice 7 days after sham or uninephrectomy surgery
Techniques Used:

Figure Legend Snippet: The enlargement of proximal tubules and glomeruli induced by unilateral nephrectomy (UNX) was inhibited in Pik3c3 hypomorphic (Hypo) mice. UNX-induced renal glomerular hypertrophy and proximal tubular hypertrophy were assessed by double immunofluorescence staining with synaptopodin to highlight glomeruli in red and Lotus tetragonolobus lectin (LTL) to label renal proximal tubules in green (A). The area of proximal tubules (B) and area of glomeruli (C) were measured as detailed in methods (n = 7 mice/group). Values are means ± SE. P values for the compared groups are indicated. N.S., not statistically significant; WT, wild type.
Techniques Used: Double Immunofluorescence Staining

Figure Legend Snippet: The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice inhibits the mammalian target of rapamycin complex 1 signaling pathway. Immunoblot analysis of renal cortical homogenates revealed a graded reduction of unilateral nephrectomy (UNX)-induced ribosomal protein S6 (rpS6) phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (A). Each lane represents one sample from the left kidney of a right uninephrectomized or sham-operated individual mouse. Immunohistochemistry (IHC) staining confirmed the graded inhibition of UNX-induced rpS6 phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (B). Shown are representative blots and images from n = 7 mice/group with similar results.
Techniques Used: Expressing, Western Blot, Immunohistochemistry, Inhibition

Figure Legend Snippet: A schematic summary illustrating the main concept of the present work demonstrating that Pik3c3 plays a pivotal role in mediating nephron deficit-induced compensatory nephron hypertrophy (CNH). Specifically, using a mouse model of uninephrectomy (UNX) to induce a 50% nephron reduction, the present study provides the first definitive evidence showing that Pikc3c plays an indispensable role in regulating the degree of CNH by generating Pik3c3 hypomorphic mouse model. Our hypertrophy data indicate that the degree of UNX-induced CNH is tightly regulated by the expression level of Pik3c3. Together with our previous demonstration that phosphorylated ribosomal protein S6 (rpS6) is a downstream effector of the mammalian target of rapamycin complex 1 (mTORC1)-S6 kinase 1 (S6K1) signaling pathway mediating CNH (53), our signaling data examining the level of rpS6 phosphorylation in the present study indicate that Pik3c3 functions upstream of the mTORC1-S6K1-rpS6 pathway mediating increased protein synthesis to drive the development of CNH.
Techniques Used: Expressing
antibodies against pik3c3 z r016 (Echelon Biosciences)


Structured Review

Antibodies Against Pik3c3 Z R016, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against pik3c3 z r016/product/Echelon Biosciences
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy"
Article Title: The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy
Journal: American Journal of Physiology - Renal Physiology
doi: 10.1152/ajprenal.00381.2019

Figure Legend Snippet: Generation of a Pik3c3 hypomorphic mouse model. Schematic illustration of the wild-type allele, targeting vector, and hypomorphic allele of the mouse Pik3c3 gene, which enabled us to generate homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice and heterozygous Pik3c3-hypomorphic (Pik3c3Hypo/WT) mice to compare with their wild-type (Pik3c3WT/WT) littermates (A). Both immunoblot (B) and immunohistochemistry staining (C) indicated that Pik3c3Hypo/Hypo mice express a markedly lower level of Pik3c3 than Pik3c3Hypo/WT littermates, which already express a lower level of Pik3c3 than Pik3c3WT/WT littermates. E, embroynic day. Shown are representative blots and images from n = 6–8 mice/genotype group with similar results.
Techniques Used: Plasmid Preparation, Western Blot, Immunohistochemistry, Staining

Figure Legend Snippet: The reduction of Pik3c3 expression also occurs in other organs of the Pik3c3 hypomorphic mouse model. Immunoblot analysis indicated that homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice (Homo) express a markedly lower level of Pik3c3 than heterozygous Pik3c3 hypomorphic (Pik3c3Hypo/WT) littermates (Het), which already express a lower level of Pik3c3 than wild-type (Pik3c3WT/WT) littermates (WT). Of note, although an equal amount (10 or 20 μg) of total protein from different tissue homogenates was loaded, both β-actin and α-tubulin (two commonly used loading controls) were expressed at considerably different levels in different tissues, reminiscent to a previous report (28). However, expression levels of either β-actin or α-tubulin in the same type of tissues examined (including spleen, brain, lung, heart, liver, pancreas, testis, and adipose tissue) were comparable among WT, Het, and Homo Pik3c3 hypomorphic mice, validating the comparison of Pik3c3 expression levels as well as the equal loading of total protein. Shown are representative blots from n = 6 mice/genotype group with similar results.
Techniques Used: Expressing, Western Blot

Figure Legend Snippet: The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice did not cause any apparent phenotype. Pik3c3Hypo/Hypo mice had a mean body weight (A), left kidney weight (B), right kidney weight (C), and left and right kidney-to-body weight ratio (D) that were not statistically different from those of Pik3c3Hypo/WT and Pik3c3WT/WT littermates. Values are means ± SE; n = 7 mice for each group. Furthermore, hematoxylin and eosin staining revealed no difference in renal histology among Pik3c3Hypo/Hypo, Pik3c3Hypo/WT, and Pik3c3WT/WT mice (E). Shown are representative images from n = 6 for each group with similar results.
Techniques Used: Expressing, Staining

Figure Legend Snippet: Body weight, left kidney weight, left kidney-to-body weight ratio, BUN, and serum creatinine levels in Pik3c3 WT/WT , Pik3c3 Hypo/WT , and Pik3c3 Hypo/Hypo mice 7 days after sham or uninephrectomy surgery
Techniques Used:

Figure Legend Snippet: The enlargement of proximal tubules and glomeruli induced by unilateral nephrectomy (UNX) was inhibited in Pik3c3 hypomorphic (Hypo) mice. UNX-induced renal glomerular hypertrophy and proximal tubular hypertrophy were assessed by double immunofluorescence staining with synaptopodin to highlight glomeruli in red and Lotus tetragonolobus lectin (LTL) to label renal proximal tubules in green (A). The area of proximal tubules (B) and area of glomeruli (C) were measured as detailed in methods (n = 7 mice/group). Values are means ± SE. P values for the compared groups are indicated. N.S., not statistically significant; WT, wild type.
Techniques Used: Double Immunofluorescence Staining

Figure Legend Snippet: The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice inhibits the mammalian target of rapamycin complex 1 signaling pathway. Immunoblot analysis of renal cortical homogenates revealed a graded reduction of unilateral nephrectomy (UNX)-induced ribosomal protein S6 (rpS6) phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (A). Each lane represents one sample from the left kidney of a right uninephrectomized or sham-operated individual mouse. Immunohistochemistry (IHC) staining confirmed the graded inhibition of UNX-induced rpS6 phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (B). Shown are representative blots and images from n = 7 mice/group with similar results.
Techniques Used: Expressing, Western Blot, Immunohistochemistry, Inhibition

Figure Legend Snippet: A schematic summary illustrating the main concept of the present work demonstrating that Pik3c3 plays a pivotal role in mediating nephron deficit-induced compensatory nephron hypertrophy (CNH). Specifically, using a mouse model of uninephrectomy (UNX) to induce a 50% nephron reduction, the present study provides the first definitive evidence showing that Pikc3c plays an indispensable role in regulating the degree of CNH by generating Pik3c3 hypomorphic mouse model. Our hypertrophy data indicate that the degree of UNX-induced CNH is tightly regulated by the expression level of Pik3c3. Together with our previous demonstration that phosphorylated ribosomal protein S6 (rpS6) is a downstream effector of the mammalian target of rapamycin complex 1 (mTORC1)-S6 kinase 1 (S6K1) signaling pathway mediating CNH (53), our signaling data examining the level of rpS6 phosphorylation in the present study indicate that Pik3c3 functions upstream of the mTORC1-S6K1-rpS6 pathway mediating increased protein synthesis to drive the development of CNH.
Techniques Used: Expressing
pik3c3 antibody (Echelon Biosciences)


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1) Product Images from "Renal developmental genes are differentially regulated after unilateral ureteral obstruction in neonatal and adult mice"
Article Title: Renal developmental genes are differentially regulated after unilateral ureteral obstruction in neonatal and adult mice
Journal: Scientific Reports
doi: 10.1038/s41598-020-76328-3

Figure Legend Snippet: Phosphatidylinositol 3 kinase class III (Pik3c3) expression is upregulated in intact opposite kidneys. Neonatal and adult mice were subjected to UUO or sham operation. Whole kidneys were processed for western blot analysis at day 1, 5, 12, and 19 after surgery. Pik3c3 expression, which controls compensatory hypertrophy, was strongly upregulated in adult intact opposite kidneys ( a and b ) and less in neonatal IO-kidneys ( a and c ). The shown western blot images are cropped, for uncropped western blots see Supplementary Fig. – online. For additional blots on Pik3c3 expression in UUO and sham-operated kidneys see Supplementary Figure . For details on significance between groups see Supplementary Table online. n = 3; * p < 0.05. Significance markings reflect the differences between groups, compared to each other on the same points in time. The images for ( a ) were rearranged for uniform presentation. The brightness of the western blot images was changed after the analysis for uniform presentation.
Techniques Used: Expressing, Western Blot