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anti hvps34  (Echelon Biosciences)


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    Structured Review

    Echelon Biosciences anti hvps34
    Anti Hvps34, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hvps34/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
    anti hvps34 - by Bioz Stars, 2025-02
    92/100 stars

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    Echelon Biosciences anti hvps34
    Anti Hvps34, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hvps34/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
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    92
    Echelon Biosciences anti vps34 antibody
    ( A ) <t>Vps34,</t> GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).
    Anti Vps34 Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vps34 antibody/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
    anti vps34 antibody - by Bioz Stars, 2025-02
    92/100 stars
      Buy from Supplier

    92
    Echelon Biosciences anti vps34
    ( A ) <t>Vps34,</t> GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).
    Anti Vps34, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vps34/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
    anti vps34 - by Bioz Stars, 2025-02
    92/100 stars
      Buy from Supplier

    92
    Echelon Biosciences anti hvps34 antibody
    ( A ) <t>Vps34,</t> GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).
    Anti Hvps34 Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hvps34 antibody/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
    anti hvps34 antibody - by Bioz Stars, 2025-02
    92/100 stars
      Buy from Supplier

    92
    Echelon Biosciences anti hpik3c3 vps34 antibody
    ( A ) <t>Vps34,</t> GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).
    Anti Hpik3c3 Vps34 Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hpik3c3 vps34 antibody/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
    anti hpik3c3 vps34 antibody - by Bioz Stars, 2025-02
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    92
    Echelon Biosciences vps34
    a Autophagy-related protein levels after treatment confirmed by western blot. b Schematic images of the autophagy initiation step involved in NRBF2. c Coimmunoprecipitation of NRBF2 and the <t>VPS34</t> complex confirmed by western blot in GBM cell lines. d Autophagic flux analysis using the mCherry-EGFP reporter. A172 and U-87 MG cells transfected with mCherry-GFP-LC3B were treated with IR (3 Gy), siNRBF2 or both. The mean numbers of autophagosomes and autolysosomes are represented by yellow and red dots, respectively, in the merged images. The images were quantified using ImageJ ( n = 5). Scale bars, 10 µm. e IHC of LC3B in coronal sections from U-87 WT or NRBF2 knockout orthotopic xenograft mouse models treated with radiation (2 Gy × 5). Scale bars, 10 μm. ** p < 0.01, *** p < 0.001, **** p < 0.0001 using an unpaired t test.
    Vps34, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vps34/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
    vps34 - by Bioz Stars, 2025-02
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    Image Search Results


    ( A ) Vps34, GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).

    Journal: PLoS ONE

    Article Title: Resistance to Inhibitors of Cholinesterase (Ric)-8A and Gα i Contribute to Cytokinesis Abscission by Controlling Vacuolar Protein-Sorting (Vps)34 Activity

    doi: 10.1371/journal.pone.0086680

    Figure Lengend Snippet: ( A ) Vps34, GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).

    Article Snippet: IP was performed with anti-Vps34 antibody (Echelon).

    Techniques: Western Blot, Transfection, Expressing, Immunoprecipitation, Purification

    ( A ) Vps34, GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).

    Journal: PLoS ONE

    Article Title: Resistance to Inhibitors of Cholinesterase (Ric)-8A and Gα i Contribute to Cytokinesis Abscission by Controlling Vacuolar Protein-Sorting (Vps)34 Activity

    doi: 10.1371/journal.pone.0086680

    Figure Lengend Snippet: ( A ) Vps34, GFP and actin immunoblots of cell lysates immunoprecipitate from HeLa cells transiently transfected with vectors expressing YFP, Gα i3 wt-YFP, or Gα i3 QL-YFP. Experiments were repeated twice with similar results. ( B ) 3D reconstruction of confocal images of HeLa cells expressing GFP-Ric-8A immunostained for endogenous Vps34. Scale bar is 5 µm. The right panels show an electronic magnification of midbody area (white square). ( C–D ) Co-immunoprecipitation of endogenous Vps34 and endogenous Ric-8A in HeLa cells. The experiment was repeated 3 times with similar results. ( E ) Asynchronous HeLa cells lysates treated with PTX (200 ng/mL, 3 h) or with its vehicle were immunoprecipitated using anti-Vps34 antibody and immunoblotted for Ric-8A or Vps34. ( F ) Quantification of PtdIns(3)P production after purification of endogenous Vps34 kinase in asynchronous or G2/M enriched HeLa cells treated with PTX (200 ng/mL, 3 h) or siRNA control versus siRNA Ric-8A. Quantification was performed from 4 independent experiments (*, p<0.05).

    Article Snippet: Primary antibodies used for western blotting were the following: anti Actin-HRP conjugated (Sigma, 1∶20,000), anti GFP (Cell Signaling, 1∶1,000), anti cyclinB1 (Cell Signaling, 1∶1,000), anti phospho-p190-progesterone receptor (Invitrogen, 0.5 µg for IP), anti-ubiquitin K63 linked-HRP conjugated (Enzo, 1∶1,000); anti total ubiquitin-HRP conjugated (Santa Cruz, P4D1, 1∶1,000); anti-Gα i3 rabbit polyclonal (Santa Cruz, 1∶500), anti-Gβ 1 rabbit polyclonal (Santa Cruz, 1∶50), anti-Gγ5 rabbit polyclonal (Santa Cruz, S14, 1∶100), anti-Ric-8A rabbit polyclonal (kind gift from Dr. G. Tall, University of Rochester, 1∶20,000 or Abcam, 1∶1,000 for WB or 0.5 µg for IP), anti-phospho Histone H3-Serine 10 (Cell signaling, 1∶1,000), anti-phospho serine (Cell signaling, 1∶300), anti-Vps34 (Echelon, 1∶1,000) and anti-Vps15 (Bethyl, 1 µg for IP and Abcam, 1∶1000 for WB).

    Techniques: Western Blot, Transfection, Expressing, Immunoprecipitation, Purification

    a Autophagy-related protein levels after treatment confirmed by western blot. b Schematic images of the autophagy initiation step involved in NRBF2. c Coimmunoprecipitation of NRBF2 and the VPS34 complex confirmed by western blot in GBM cell lines. d Autophagic flux analysis using the mCherry-EGFP reporter. A172 and U-87 MG cells transfected with mCherry-GFP-LC3B were treated with IR (3 Gy), siNRBF2 or both. The mean numbers of autophagosomes and autolysosomes are represented by yellow and red dots, respectively, in the merged images. The images were quantified using ImageJ ( n = 5). Scale bars, 10 µm. e IHC of LC3B in coronal sections from U-87 WT or NRBF2 knockout orthotopic xenograft mouse models treated with radiation (2 Gy × 5). Scale bars, 10 μm. ** p < 0.01, *** p < 0.001, **** p < 0.0001 using an unpaired t test.

    Journal: Experimental & Molecular Medicine

    Article Title: NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma

    doi: 10.1038/s12276-022-00873-2

    Figure Lengend Snippet: a Autophagy-related protein levels after treatment confirmed by western blot. b Schematic images of the autophagy initiation step involved in NRBF2. c Coimmunoprecipitation of NRBF2 and the VPS34 complex confirmed by western blot in GBM cell lines. d Autophagic flux analysis using the mCherry-EGFP reporter. A172 and U-87 MG cells transfected with mCherry-GFP-LC3B were treated with IR (3 Gy), siNRBF2 or both. The mean numbers of autophagosomes and autolysosomes are represented by yellow and red dots, respectively, in the merged images. The images were quantified using ImageJ ( n = 5). Scale bars, 10 µm. e IHC of LC3B in coronal sections from U-87 WT or NRBF2 knockout orthotopic xenograft mouse models treated with radiation (2 Gy × 5). Scale bars, 10 μm. ** p < 0.01, *** p < 0.001, **** p < 0.0001 using an unpaired t test.

    Article Snippet: Antibodies specific for VPS34 were purchased from Echelon Biosciences (Echelon Biosciences, Salt Lake City, UT, USA), and antibodies specific for ATG14L were purchased from MBL (MBL, Tokyo, Japan).

    Techniques: Western Blot, Transfection, Knock-Out