Journal: iScience

Article Title: Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide

doi: 10.1016/j.isci.2022.104624

Figure Lengend Snippet:

Article Snippet: anti-LBPA , Echelon Bioscience , Cat#: z-PLBPA; RRID: AB_11129226.

Techniques: Transduction, Recombinant, Protease Inhibitor, Software

Journal: bioRxiv

Article Title: Caveolae couple mechanical stress to integrin recycling and activation

doi: 10.1101/2022.04.27.489710

Figure Lengend Snippet: (A-D) Confocal microscopy images of Cav1KO MEFs stained for CD147 (green in A and C), active β1-integrin (9EG7 antibody, green in B and D) and EEA1 (magenta), either non-treated (A and B) or treated with siRNA against HOOK1 for 48h (C and D). (E) Quantification of colocalization between EEA1 and CD147 or 9EG7 normalized to control, expressed as Pearson’s correlation coefficient; n ≥ 20 cells per condition. (F-I) Confocal microscopy images of wild type (F and H) or Cav1KO MEFs (G and I), stained for active β1-integrin (9EG7 antibody, green) and LBPA (magenta), either non-transfected (F and G) or transfected with Rab11 N124I dominant negative mutant for 48h (H and I). (J) Quantification of colocalization between LBPA and 9EG7 normalized by each control, expressed as Pearson’s correlation coefficient; n ≥ 20 cells per condition. (K-N) Confocal microscopy images of wild type (K and M) or Cav1KO MEFs (L and N), stained for active β1-integrin (9EG7 antibody, green) and EEA-1 (magenta), either non-transfected (K and L) or transfected with Rab4 S22N dominant negative mutant for 48h (M and N). (O) Quantification of colocalization between EEA-1 and 9EG7 normalized to each control, expressed as Pearson’s correlation coefficient; n ≥ 30 cells per condition. Colocalization was analyzed using the plugin Intensity Correlation Analysis (Fiji ). Statistical significance of differences across indicated conditions was assessed by t -test: * p <0.05; P** p <0.01; *** p <0.001 N. S., non-significant. See also Raw Data and which includes the raw data of experiments from and .

Article Snippet: The following primary antibodies were used: rat monoclonal anti-mouse total β1-integrin (clone MB1.2, MAB1997 Millipore); rat monoclonal anti-mouse β1-integrin, activated (clone 9EG7, BD Pharmingen™); Alexa 488 conjugated anti-integrin β1, activated (clone HUTS-4, MAB2079-AF488 Millipore); rabbit polyclonal anti-mouse PTRF (Abcam); rabbit monoclonal anti-mouse caveolin-1 (Cell Signaling, #3238); mouse monoclonal anti-tubulin (Abcam, clone DM1A); rabbit monoclonal anti-CD147 (Invitrogen, clone JF1-045), mouse monoclonal anti-EEA-1 (BD transduction, clone 14) and mouse monoclonal anti-LBPA (Echelon Z-SLBPA).

Techniques: Confocal Microscopy, Staining, Transfection, Dominant Negative Mutation