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    z plbpa  (Echelon Biosciences)


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    anti lbpa z plbpa  (Echelon Biosciences)


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    z-plbpa  (Echelon Biosciences)


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    Echelon Biosciences z-plbpa
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    z plbpa  (Echelon Biosciences)


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    Echelon Biosciences z plbpa
    Antibodies/IF Reagents.
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    1) Product Images from "Rgp1 contributes to craniofacial cartilage development and Rab8a-mediated collagen II secretion"

    Article Title: Rgp1 contributes to craniofacial cartilage development and Rab8a-mediated collagen II secretion

    Journal: Frontiers in Endocrinology

    doi: 10.3389/fendo.2023.1120420

    Antibodies/IF Reagents.
    Figure Legend Snippet: Antibodies/IF Reagents.

    Techniques Used:

    z plbpa  (Echelon Biosciences)


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    1) Product Images from "Lysosomal trafficking of the glucose transporter GLUT1 requires sequential regulation by TXNIP and ubiquitin"

    Article Title: Lysosomal trafficking of the glucose transporter GLUT1 requires sequential regulation by TXNIP and ubiquitin

    Journal: iScience

    doi: 10.1016/j.isci.2023.106150


    Figure Legend Snippet:

    Techniques Used: Purification, Produced, Recombinant, Infection, Stable Transfection, Expressing, Diagnostic Assay, CRISPR, Plasmid Preparation, Software

    mouse anti mouse lbpa z plbpa  (Echelon Biosciences)


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    mouse anti mouse lbpa z plbpa  (Echelon Biosciences)


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    Echelon Biosciences mouse anti mouse lbpa z plbpa
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    mouse monoclonal antibody to lbpa  (Echelon Biosciences)


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    mouse monoclonal anti lbpa  (Echelon Biosciences)


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    Echelon Biosciences mouse monoclonal anti lbpa
    ( A–D ) Confocal microscopy images of Cav1KO mouse embryonic fibroblasts (MEFs) stained <t>for</t> <t>CD147</t> (green in A and C), active β1-integrin (9EG7 antibody, green in B and D) and EEA1 (magenta), either non-treated ( A and B ) or treated with siRNA against HOOK1 for 48 hr ( C and D ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( E ) Quantification of colocalization between EEA1 and CD147 or 9EG7 normalized to control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( F–I ) Confocal microscopy images of wild type ( F and H ) or Cav1KO MEFs ( G and I ), stained for active β1-integrin (9EG7 antibody, green) and lysobisphosphatidic acid <t>(LBPA)</t> (magenta), either non-transfected ( F and G ) or transfected with Rab11 N124I dominant negative mutant for 48 hr ( H and I ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( J ) Quantification of colocalization between LBPA and 9EG7 normalized by each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( K–N ) Confocal microscopy images of wild type ( K and M ) or Cav1KO MEFs ( L and N ), stained for active β1-integrin (9EG7 antibody, green) and EEA-1 (magenta), either non-transfected ( K and L ) or transfected with Rab4 S22N dominant negative mutant for 48 hr ( M and N ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( O ) Quantification of colocalization between EEA-1 and 9EG7 normalized to each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥30 cells per condition. Colocalization was analyzed using the plugin intensity correlation analysis (Fiji ). ( P–V ) Confocal microscopy images of wild type MEFs ( P–R ) and Cav1KO MEFs ( T–V ) incubated with anti-active β1-Alexa 488 antibody (green) for 1 hr at 4°C followed by 3 min endocytosis at 37°C. Remaining surface fluorescence was removed by acid stripping prior to fixation with paraformaldehyde (PFA) at 4%. Scale bar = 10 µm. Quantification of mean fluorescence intensity of endocytosed active β1-integrin in Cav1WT ( S ) or Cav1KO MEFs ( W ). Values were normalized to mean fluorescence intensity of control; n≥30 cells per genotype and condition. Data are presented as mean values +/- SEM. Statistical significance of differences across indicated conditions was assessed by t-test: * p<0.05; P**p<0.01; ***p<0.001 N. S., non-significant. Figure 6—source data 1. Raw data of experiments from .
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    1) Product Images from "Caveolae couple mechanical stress to integrin recycling and activation"

    Article Title: Caveolae couple mechanical stress to integrin recycling and activation

    Journal: eLife

    doi: 10.7554/eLife.82348

    ( A–D ) Confocal microscopy images of Cav1KO mouse embryonic fibroblasts (MEFs) stained for CD147 (green in A and C), active β1-integrin (9EG7 antibody, green in B and D) and EEA1 (magenta), either non-treated ( A and B ) or treated with siRNA against HOOK1 for 48 hr ( C and D ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( E ) Quantification of colocalization between EEA1 and CD147 or 9EG7 normalized to control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( F–I ) Confocal microscopy images of wild type ( F and H ) or Cav1KO MEFs ( G and I ), stained for active β1-integrin (9EG7 antibody, green) and lysobisphosphatidic acid (LBPA) (magenta), either non-transfected ( F and G ) or transfected with Rab11 N124I dominant negative mutant for 48 hr ( H and I ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( J ) Quantification of colocalization between LBPA and 9EG7 normalized by each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( K–N ) Confocal microscopy images of wild type ( K and M ) or Cav1KO MEFs ( L and N ), stained for active β1-integrin (9EG7 antibody, green) and EEA-1 (magenta), either non-transfected ( K and L ) or transfected with Rab4 S22N dominant negative mutant for 48 hr ( M and N ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( O ) Quantification of colocalization between EEA-1 and 9EG7 normalized to each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥30 cells per condition. Colocalization was analyzed using the plugin intensity correlation analysis (Fiji ). ( P–V ) Confocal microscopy images of wild type MEFs ( P–R ) and Cav1KO MEFs ( T–V ) incubated with anti-active β1-Alexa 488 antibody (green) for 1 hr at 4°C followed by 3 min endocytosis at 37°C. Remaining surface fluorescence was removed by acid stripping prior to fixation with paraformaldehyde (PFA) at 4%. Scale bar = 10 µm. Quantification of mean fluorescence intensity of endocytosed active β1-integrin in Cav1WT ( S ) or Cav1KO MEFs ( W ). Values were normalized to mean fluorescence intensity of control; n≥30 cells per genotype and condition. Data are presented as mean values +/- SEM. Statistical significance of differences across indicated conditions was assessed by t-test: * p<0.05; P**p<0.01; ***p<0.001 N. S., non-significant. Figure 6—source data 1. Raw data of experiments from .
    Figure Legend Snippet: ( A–D ) Confocal microscopy images of Cav1KO mouse embryonic fibroblasts (MEFs) stained for CD147 (green in A and C), active β1-integrin (9EG7 antibody, green in B and D) and EEA1 (magenta), either non-treated ( A and B ) or treated with siRNA against HOOK1 for 48 hr ( C and D ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( E ) Quantification of colocalization between EEA1 and CD147 or 9EG7 normalized to control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( F–I ) Confocal microscopy images of wild type ( F and H ) or Cav1KO MEFs ( G and I ), stained for active β1-integrin (9EG7 antibody, green) and lysobisphosphatidic acid (LBPA) (magenta), either non-transfected ( F and G ) or transfected with Rab11 N124I dominant negative mutant for 48 hr ( H and I ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( J ) Quantification of colocalization between LBPA and 9EG7 normalized by each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( K–N ) Confocal microscopy images of wild type ( K and M ) or Cav1KO MEFs ( L and N ), stained for active β1-integrin (9EG7 antibody, green) and EEA-1 (magenta), either non-transfected ( K and L ) or transfected with Rab4 S22N dominant negative mutant for 48 hr ( M and N ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( O ) Quantification of colocalization between EEA-1 and 9EG7 normalized to each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥30 cells per condition. Colocalization was analyzed using the plugin intensity correlation analysis (Fiji ). ( P–V ) Confocal microscopy images of wild type MEFs ( P–R ) and Cav1KO MEFs ( T–V ) incubated with anti-active β1-Alexa 488 antibody (green) for 1 hr at 4°C followed by 3 min endocytosis at 37°C. Remaining surface fluorescence was removed by acid stripping prior to fixation with paraformaldehyde (PFA) at 4%. Scale bar = 10 µm. Quantification of mean fluorescence intensity of endocytosed active β1-integrin in Cav1WT ( S ) or Cav1KO MEFs ( W ). Values were normalized to mean fluorescence intensity of control; n≥30 cells per genotype and condition. Data are presented as mean values +/- SEM. Statistical significance of differences across indicated conditions was assessed by t-test: * p<0.05; P**p<0.01; ***p<0.001 N. S., non-significant. Figure 6—source data 1. Raw data of experiments from .

    Techniques Used: Confocal Microscopy, Staining, Transfection, Dominant Negative Mutation, Incubation, Fluorescence, Stripping Membranes

    anti lbpa mouse antibody 6c4  (Echelon Biosciences)


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    Echelon Biosciences anti lbpa mouse antibody 6c4
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    ( A–D ) Confocal microscopy images of Cav1KO mouse embryonic fibroblasts (MEFs) stained <t>for</t> <t>CD147</t> (green in A and C), active β1-integrin (9EG7 antibody, green in B and D) and EEA1 (magenta), either non-treated ( A and B ) or treated with siRNA against HOOK1 for 48 hr ( C and D ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( E ) Quantification of colocalization between EEA1 and CD147 or 9EG7 normalized to control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( F–I ) Confocal microscopy images of wild type ( F and H ) or Cav1KO MEFs ( G and I ), stained for active β1-integrin (9EG7 antibody, green) and lysobisphosphatidic acid <t>(LBPA)</t> (magenta), either non-transfected ( F and G ) or transfected with Rab11 N124I dominant negative mutant for 48 hr ( H and I ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( J ) Quantification of colocalization between LBPA and 9EG7 normalized by each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( K–N ) Confocal microscopy images of wild type ( K and M ) or Cav1KO MEFs ( L and N ), stained for active β1-integrin (9EG7 antibody, green) and EEA-1 (magenta), either non-transfected ( K and L ) or transfected with Rab4 S22N dominant negative mutant for 48 hr ( M and N ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( O ) Quantification of colocalization between EEA-1 and 9EG7 normalized to each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥30 cells per condition. Colocalization was analyzed using the plugin intensity correlation analysis (Fiji ). ( P–V ) Confocal microscopy images of wild type MEFs ( P–R ) and Cav1KO MEFs ( T–V ) incubated with anti-active β1-Alexa 488 antibody (green) for 1 hr at 4°C followed by 3 min endocytosis at 37°C. Remaining surface fluorescence was removed by acid stripping prior to fixation with paraformaldehyde (PFA) at 4%. Scale bar = 10 µm. Quantification of mean fluorescence intensity of endocytosed active β1-integrin in Cav1WT ( S ) or Cav1KO MEFs ( W ). Values were normalized to mean fluorescence intensity of control; n≥30 cells per genotype and condition. Data are presented as mean values +/- SEM. Statistical significance of differences across indicated conditions was assessed by t-test: * p<0.05; P**p<0.01; ***p<0.001 N. S., non-significant. Figure 6—source data 1. Raw data of experiments from .
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    ( A–D ) Confocal microscopy images of Cav1KO mouse embryonic fibroblasts (MEFs) stained <t>for</t> <t>CD147</t> (green in A and C), active β1-integrin (9EG7 antibody, green in B and D) and EEA1 (magenta), either non-treated ( A and B ) or treated with siRNA against HOOK1 for 48 hr ( C and D ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( E ) Quantification of colocalization between EEA1 and CD147 or 9EG7 normalized to control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( F–I ) Confocal microscopy images of wild type ( F and H ) or Cav1KO MEFs ( G and I ), stained for active β1-integrin (9EG7 antibody, green) and lysobisphosphatidic acid <t>(LBPA)</t> (magenta), either non-transfected ( F and G ) or transfected with Rab11 N124I dominant negative mutant for 48 hr ( H and I ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( J ) Quantification of colocalization between LBPA and 9EG7 normalized by each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( K–N ) Confocal microscopy images of wild type ( K and M ) or Cav1KO MEFs ( L and N ), stained for active β1-integrin (9EG7 antibody, green) and EEA-1 (magenta), either non-transfected ( K and L ) or transfected with Rab4 S22N dominant negative mutant for 48 hr ( M and N ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( O ) Quantification of colocalization between EEA-1 and 9EG7 normalized to each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥30 cells per condition. Colocalization was analyzed using the plugin intensity correlation analysis (Fiji ). ( P–V ) Confocal microscopy images of wild type MEFs ( P–R ) and Cav1KO MEFs ( T–V ) incubated with anti-active β1-Alexa 488 antibody (green) for 1 hr at 4°C followed by 3 min endocytosis at 37°C. Remaining surface fluorescence was removed by acid stripping prior to fixation with paraformaldehyde (PFA) at 4%. Scale bar = 10 µm. Quantification of mean fluorescence intensity of endocytosed active β1-integrin in Cav1WT ( S ) or Cav1KO MEFs ( W ). Values were normalized to mean fluorescence intensity of control; n≥30 cells per genotype and condition. Data are presented as mean values +/- SEM. Statistical significance of differences across indicated conditions was assessed by t-test: * p<0.05; P**p<0.01; ***p<0.001 N. S., non-significant. Figure 6—source data 1. Raw data of experiments from .
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    ( A–D ) Confocal microscopy images of Cav1KO mouse embryonic fibroblasts (MEFs) stained for CD147 (green in A and C), active β1-integrin (9EG7 antibody, green in B and D) and EEA1 (magenta), either non-treated ( A and B ) or treated with siRNA against HOOK1 for 48 hr ( C and D ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( E ) Quantification of colocalization between EEA1 and CD147 or 9EG7 normalized to control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( F–I ) Confocal microscopy images of wild type ( F and H ) or Cav1KO MEFs ( G and I ), stained for active β1-integrin (9EG7 antibody, green) and lysobisphosphatidic acid (LBPA) (magenta), either non-transfected ( F and G ) or transfected with Rab11 N124I dominant negative mutant for 48 hr ( H and I ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( J ) Quantification of colocalization between LBPA and 9EG7 normalized by each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( K–N ) Confocal microscopy images of wild type ( K and M ) or Cav1KO MEFs ( L and N ), stained for active β1-integrin (9EG7 antibody, green) and EEA-1 (magenta), either non-transfected ( K and L ) or transfected with Rab4 S22N dominant negative mutant for 48 hr ( M and N ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( O ) Quantification of colocalization between EEA-1 and 9EG7 normalized to each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥30 cells per condition. Colocalization was analyzed using the plugin intensity correlation analysis (Fiji ). ( P–V ) Confocal microscopy images of wild type MEFs ( P–R ) and Cav1KO MEFs ( T–V ) incubated with anti-active β1-Alexa 488 antibody (green) for 1 hr at 4°C followed by 3 min endocytosis at 37°C. Remaining surface fluorescence was removed by acid stripping prior to fixation with paraformaldehyde (PFA) at 4%. Scale bar = 10 µm. Quantification of mean fluorescence intensity of endocytosed active β1-integrin in Cav1WT ( S ) or Cav1KO MEFs ( W ). Values were normalized to mean fluorescence intensity of control; n≥30 cells per genotype and condition. Data are presented as mean values +/- SEM. Statistical significance of differences across indicated conditions was assessed by t-test: * p<0.05; P**p<0.01; ***p<0.001 N. S., non-significant. Figure 6—source data 1. Raw data of experiments from .

    Journal: eLife

    Article Title: Caveolae couple mechanical stress to integrin recycling and activation

    doi: 10.7554/eLife.82348

    Figure Lengend Snippet: ( A–D ) Confocal microscopy images of Cav1KO mouse embryonic fibroblasts (MEFs) stained for CD147 (green in A and C), active β1-integrin (9EG7 antibody, green in B and D) and EEA1 (magenta), either non-treated ( A and B ) or treated with siRNA against HOOK1 for 48 hr ( C and D ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( E ) Quantification of colocalization between EEA1 and CD147 or 9EG7 normalized to control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( F–I ) Confocal microscopy images of wild type ( F and H ) or Cav1KO MEFs ( G and I ), stained for active β1-integrin (9EG7 antibody, green) and lysobisphosphatidic acid (LBPA) (magenta), either non-transfected ( F and G ) or transfected with Rab11 N124I dominant negative mutant for 48 hr ( H and I ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( J ) Quantification of colocalization between LBPA and 9EG7 normalized by each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥20 cells per condition. ( K–N ) Confocal microscopy images of wild type ( K and M ) or Cav1KO MEFs ( L and N ), stained for active β1-integrin (9EG7 antibody, green) and EEA-1 (magenta), either non-transfected ( K and L ) or transfected with Rab4 S22N dominant negative mutant for 48 hr ( M and N ). Insets (below each panel) show colocalizing structures. Scale bar = 10 µm. ( O ) Quantification of colocalization between EEA-1 and 9EG7 normalized to each control, expressed as Pearson’s correlation coefficient normalized by the mean of the corresponding control condition as indicated; n≥30 cells per condition. Colocalization was analyzed using the plugin intensity correlation analysis (Fiji ). ( P–V ) Confocal microscopy images of wild type MEFs ( P–R ) and Cav1KO MEFs ( T–V ) incubated with anti-active β1-Alexa 488 antibody (green) for 1 hr at 4°C followed by 3 min endocytosis at 37°C. Remaining surface fluorescence was removed by acid stripping prior to fixation with paraformaldehyde (PFA) at 4%. Scale bar = 10 µm. Quantification of mean fluorescence intensity of endocytosed active β1-integrin in Cav1WT ( S ) or Cav1KO MEFs ( W ). Values were normalized to mean fluorescence intensity of control; n≥30 cells per genotype and condition. Data are presented as mean values +/- SEM. Statistical significance of differences across indicated conditions was assessed by t-test: * p<0.05; P**p<0.01; ***p<0.001 N. S., non-significant. Figure 6—source data 1. Raw data of experiments from .

    Article Snippet: The following primary antibodies were used: mouse monoclonal anti-transferrin receptor (H68.4, Catalog # 13–6800), rat monoclonal anti-mouse total β1-integrin (clone MB1.2, MAB1997 Millipore); rat monoclonal anti-mouse β1-integrin, activated (clone 9EG7, BD Pharmingen); Alexa 488 conjugated anti-integrin β1, activated (clone HUTS-4, MAB2079-AF488 Millipore); rabbit polyclonal anti-mouse PTRF (Abcam); rabbit monoclonal anti-mouse caveolin-1 (Cell Signaling, #3238); mouse monoclonal anti-tubulin (Abcam, clone DM1A); rabbit monoclonal anti-CD147 (Invitrogen, clone JF1-045), mouse monoclonal anti-EEA-1 (BD transduction, clone 14); mouse monoclonal anti-LBPA (Echelon Z-SLBPA); mouse monoclonal anti-p190RhoGAP (Upstate, clone D2D6, 1:1000); rabbit monoclonal anti-mouse Caveolin-1 (Cell signaling, 1:1000); mouse monoclonal anti-alpha tubulin (ab7291, Abcam, 1:10.000); mouse monoclonal anti-Talin (Sigma Aldrich, clone 8d4, 1:200); and mouse anti-CD44 (clone 5035–41.1D, Novus Biologicals).

    Techniques: Confocal Microscopy, Staining, Transfection, Dominant Negative Mutation, Incubation, Fluorescence, Stripping Membranes