z-p045  (Echelon Biosciences)


Bioz Verified Symbol Echelon Biosciences is a verified supplier
Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences z-p045
    Z P045, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z-p045/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z-p045 - by Bioz Stars, 2024-04
    93/100 stars

    Images

    z p045  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Echelon Biosciences z p045

    Z P045, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z p045/product/Echelon Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z p045 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Mechanical activation of TWIK-related potassium channel by nanoscopic movement and rapid second messenger signaling"

    Article Title: Mechanical activation of TWIK-related potassium channel by nanoscopic movement and rapid second messenger signaling

    Journal: eLife

    doi: 10.7554/eLife.89465


    Figure Legend Snippet:

    Techniques Used: Transfection, Construct, Recombinant

    pi 4 5 p2 echelon biosciences z p045  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Echelon Biosciences pi 4 5 p2 echelon biosciences z p045
    Pi 4 5 P2 Echelon Biosciences Z P045, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi 4 5 p2 echelon biosciences z p045/product/Echelon Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pi 4 5 p2 echelon biosciences z p045 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    pi 4 5 p2 echelon biosciences z p045  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Echelon Biosciences pi 4 5 p2 echelon biosciences z p045
    α-Synuclein and <t>PI(4,5)P2</t> colocalize on the plasma membrane. A , membrane sheets from PTK2 cells immunostained for α-synuclein ( red ), clathrin heavy chain ( green ), and PI(4,5)P2 ( blue ), with different classes of PI(4,5)P2 puncta highlighted (t = 0). B , relative distribution of α-synuclein and clathrin in the PI(4,5)P2 positive puncta. N = 2 independent experiments with four cells analyzed per experiment (3123 total puncta). Scale bar represents 1 μm.
    Pi 4 5 P2 Echelon Biosciences Z P045, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi 4 5 p2 echelon biosciences z p045/product/Echelon Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pi 4 5 p2 echelon biosciences z p045 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "α-Synuclein colocalizes with AP180 and affects the size of clathrin lattices"

    Article Title: α-Synuclein colocalizes with AP180 and affects the size of clathrin lattices

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2023.105091

    α-Synuclein and PI(4,5)P2 colocalize on the plasma membrane. A , membrane sheets from PTK2 cells immunostained for α-synuclein ( red ), clathrin heavy chain ( green ), and PI(4,5)P2 ( blue ), with different classes of PI(4,5)P2 puncta highlighted (t = 0). B , relative distribution of α-synuclein and clathrin in the PI(4,5)P2 positive puncta. N = 2 independent experiments with four cells analyzed per experiment (3123 total puncta). Scale bar represents 1 μm.
    Figure Legend Snippet: α-Synuclein and PI(4,5)P2 colocalize on the plasma membrane. A , membrane sheets from PTK2 cells immunostained for α-synuclein ( red ), clathrin heavy chain ( green ), and PI(4,5)P2 ( blue ), with different classes of PI(4,5)P2 puncta highlighted (t = 0). B , relative distribution of α-synuclein and clathrin in the PI(4,5)P2 positive puncta. N = 2 independent experiments with four cells analyzed per experiment (3123 total puncta). Scale bar represents 1 μm.

    Techniques Used: Membrane

    z-p045  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences z-p045
    Z P045, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z-p045/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z-p045 - by Bioz Stars, 2024-04
    93/100 stars

    Images

    z-p045  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences z-p045
    Z P045, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z-p045/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z-p045 - by Bioz Stars, 2024-04
    93/100 stars

    Images

    z-p045  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences z-p045
    Z P045, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z-p045/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z-p045 - by Bioz Stars, 2024-04
    93/100 stars

    Images

    pip2  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences pip2
    A , Chemotactic WT or KO MEF ( long arrows : chemotaxis direction) transiently expressing the PIP3 reporter, GFP-PH-AKT, and stained for F-actin, showing enrichment of PIP3 in the leading edge filopodia of KO cells. Short arrows , leading edge lamellipodia (WT cells) or filopodia (KO cells). B , Re-expression of FL-, but not ΔPBD-, SSeCKS-GFP rescues leading edge lamellipodia formation in KO MEF and suppresses leading edge enrichment of the GFP-PH-AKT reporter. Scale bar , 10 µm. C , Quantification of leading edge GFP-PH-AKT levels (normalized to total cell GFP) determined for 3 fields containing at least 10 cells/field in 2 independent experiments. Error bars , S.E. *, p<0.01, **, p<0.005. D , <t>PIP2</t> or PIP3 in chemotactic leading edges of WT or KO MEF by IFA or quantified as in E . **, p<0.01. Scale bar , 10 µm.
    Pip2, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pip2/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pip2 - by Bioz Stars, 2024-04
    93/100 stars

    Images

    1) Product Images from "Suppression of Chemotaxis by SSeCKS via Scaffolding of Phosphoinositol Phosphates and the Recruitment of the Cdc42 GEF, Frabin, to the Leading Edge"

    Article Title: Suppression of Chemotaxis by SSeCKS via Scaffolding of Phosphoinositol Phosphates and the Recruitment of the Cdc42 GEF, Frabin, to the Leading Edge

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0111534

    A , Chemotactic WT or KO MEF ( long arrows : chemotaxis direction) transiently expressing the PIP3 reporter, GFP-PH-AKT, and stained for F-actin, showing enrichment of PIP3 in the leading edge filopodia of KO cells. Short arrows , leading edge lamellipodia (WT cells) or filopodia (KO cells). B , Re-expression of FL-, but not ΔPBD-, SSeCKS-GFP rescues leading edge lamellipodia formation in KO MEF and suppresses leading edge enrichment of the GFP-PH-AKT reporter. Scale bar , 10 µm. C , Quantification of leading edge GFP-PH-AKT levels (normalized to total cell GFP) determined for 3 fields containing at least 10 cells/field in 2 independent experiments. Error bars , S.E. *, p<0.01, **, p<0.005. D , PIP2 or PIP3 in chemotactic leading edges of WT or KO MEF by IFA or quantified as in E . **, p<0.01. Scale bar , 10 µm.
    Figure Legend Snippet: A , Chemotactic WT or KO MEF ( long arrows : chemotaxis direction) transiently expressing the PIP3 reporter, GFP-PH-AKT, and stained for F-actin, showing enrichment of PIP3 in the leading edge filopodia of KO cells. Short arrows , leading edge lamellipodia (WT cells) or filopodia (KO cells). B , Re-expression of FL-, but not ΔPBD-, SSeCKS-GFP rescues leading edge lamellipodia formation in KO MEF and suppresses leading edge enrichment of the GFP-PH-AKT reporter. Scale bar , 10 µm. C , Quantification of leading edge GFP-PH-AKT levels (normalized to total cell GFP) determined for 3 fields containing at least 10 cells/field in 2 independent experiments. Error bars , S.E. *, p<0.01, **, p<0.005. D , PIP2 or PIP3 in chemotactic leading edges of WT or KO MEF by IFA or quantified as in E . **, p<0.01. Scale bar , 10 µm.

    Techniques Used: Chemotaxis Assay, Expressing, Staining

    In WT (SSeCKS+/+) cells ( top ), the ability of SSeCKS to scaffold Src at lipid raft sites allows for local activation of PI3K/AKT signaling, thereby producing locally enriched PIP2/3. SSeCKS’ additional ability to scaffold PIPs, including PIP2/3, at the chemotactic leading edge facilitates the local activation of Rac1, resulting in lamellipodial-dependent chemotaxis. In contrast, in the absence of SSeCKS’s scaffolding of PIPs, PIP3 enriches in budding filopodia, which attracts binding of Frabin through its PH and FYVE domains. This leads to local activation of Cdc42 and the subsequent domination of growing filopodia at the leading edge. Of note is that SSeCKS also affects actin cytoskeletal modeling not addressed directly in this paper, such as the generation of thickened, longitudinal stress fibers (SF) and the loss of arc SF in SSeCKS-null cells.
    Figure Legend Snippet: In WT (SSeCKS+/+) cells ( top ), the ability of SSeCKS to scaffold Src at lipid raft sites allows for local activation of PI3K/AKT signaling, thereby producing locally enriched PIP2/3. SSeCKS’ additional ability to scaffold PIPs, including PIP2/3, at the chemotactic leading edge facilitates the local activation of Rac1, resulting in lamellipodial-dependent chemotaxis. In contrast, in the absence of SSeCKS’s scaffolding of PIPs, PIP3 enriches in budding filopodia, which attracts binding of Frabin through its PH and FYVE domains. This leads to local activation of Cdc42 and the subsequent domination of growing filopodia at the leading edge. Of note is that SSeCKS also affects actin cytoskeletal modeling not addressed directly in this paper, such as the generation of thickened, longitudinal stress fibers (SF) and the loss of arc SF in SSeCKS-null cells.

    Techniques Used: Activation Assay, Chemotaxis Assay, Scaffolding, Binding Assay

    mouse anti pi 4 5 p2 monoclonal igm antibody  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences mouse anti pi 4 5 p2 monoclonal igm antibody
    A. Confocal images and zoom boxes of PI(4)P distribution in Control and VAPA KO leader cells expressing GFP-PH-OSBP and immunostained for EEA1 or TGN46. Scale Bar: 10 µm. B. Analysis of colocalization by Pearson coefficient determination between GFP-PH-OSBP and EEA1 or TGN46 signals, quantified from images in A, in Control and VAPA KO leader cells (mean±SEM; Control: n=9 cells and 12 cells respectively; VAPA KO: n=9 cells and 12 cells respectively, from 3 independent experiments). C. Analysis of Golgi(TGN46)/cytosol (left) and Endosomes(EEA1)/Cytosol (right) ratio of GFP-PH-OSBP signal, quantified from images in A, in Control and VAPA KO leader cells (mean ±SEM; Control: n=12 cells; VAPA KO: n=11 cells, from 3 independent experiments). D. Analysis of RFP-PH-PLCδ1 peak intensity, quantified from images in F, in Control and VAPA KO leader cells (mean±SEM; Control: n=58 cells; VAPA KO: n=53 cells, from 3 independent experiments). E. Analysis of PM/Cytosol ratio of RFP-PH-PLCδ1, quantified from images in G, at the leading edge of Control and VAPA KO leader cells (mean±SEM; Control: n=27 cells; VAPA KO: n=26 cells, from 3 independent experiments). F. XZ view of confocal images of Control and VAPA KO cells in cluster immunostained for <t>PI(4,5)P2.</t> Scale Bar: 5 µm. G. Confocal images of PI(4,5)P2 distribution in Control and VAPA KO leader cells expressing RFP-PH-PLCδ1, represented as a color-coded heat map. Scale bar: 10 µm. All data were analysed using non parametric Mann-Whitney t-test (ns: non significant, ***P-values <0.001).
    Mouse Anti Pi 4 5 P2 Monoclonal Igm Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti pi 4 5 p2 monoclonal igm antibody/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti pi 4 5 p2 monoclonal igm antibody - by Bioz Stars, 2024-04
    93/100 stars

    Images

    1) Product Images from "The ER tether VAPA is required for proper cell motility and for anchoring ER-plasma membrane contact sites to focal adhesions"

    Article Title: The ER tether VAPA is required for proper cell motility and for anchoring ER-plasma membrane contact sites to focal adhesions

    Journal: bioRxiv

    doi: 10.1101/2022.10.17.512434

    A. Confocal images and zoom boxes of PI(4)P distribution in Control and VAPA KO leader cells expressing GFP-PH-OSBP and immunostained for EEA1 or TGN46. Scale Bar: 10 µm. B. Analysis of colocalization by Pearson coefficient determination between GFP-PH-OSBP and EEA1 or TGN46 signals, quantified from images in A, in Control and VAPA KO leader cells (mean±SEM; Control: n=9 cells and 12 cells respectively; VAPA KO: n=9 cells and 12 cells respectively, from 3 independent experiments). C. Analysis of Golgi(TGN46)/cytosol (left) and Endosomes(EEA1)/Cytosol (right) ratio of GFP-PH-OSBP signal, quantified from images in A, in Control and VAPA KO leader cells (mean ±SEM; Control: n=12 cells; VAPA KO: n=11 cells, from 3 independent experiments). D. Analysis of RFP-PH-PLCδ1 peak intensity, quantified from images in F, in Control and VAPA KO leader cells (mean±SEM; Control: n=58 cells; VAPA KO: n=53 cells, from 3 independent experiments). E. Analysis of PM/Cytosol ratio of RFP-PH-PLCδ1, quantified from images in G, at the leading edge of Control and VAPA KO leader cells (mean±SEM; Control: n=27 cells; VAPA KO: n=26 cells, from 3 independent experiments). F. XZ view of confocal images of Control and VAPA KO cells in cluster immunostained for PI(4,5)P2. Scale Bar: 5 µm. G. Confocal images of PI(4,5)P2 distribution in Control and VAPA KO leader cells expressing RFP-PH-PLCδ1, represented as a color-coded heat map. Scale bar: 10 µm. All data were analysed using non parametric Mann-Whitney t-test (ns: non significant, ***P-values <0.001).
    Figure Legend Snippet: A. Confocal images and zoom boxes of PI(4)P distribution in Control and VAPA KO leader cells expressing GFP-PH-OSBP and immunostained for EEA1 or TGN46. Scale Bar: 10 µm. B. Analysis of colocalization by Pearson coefficient determination between GFP-PH-OSBP and EEA1 or TGN46 signals, quantified from images in A, in Control and VAPA KO leader cells (mean±SEM; Control: n=9 cells and 12 cells respectively; VAPA KO: n=9 cells and 12 cells respectively, from 3 independent experiments). C. Analysis of Golgi(TGN46)/cytosol (left) and Endosomes(EEA1)/Cytosol (right) ratio of GFP-PH-OSBP signal, quantified from images in A, in Control and VAPA KO leader cells (mean ±SEM; Control: n=12 cells; VAPA KO: n=11 cells, from 3 independent experiments). D. Analysis of RFP-PH-PLCδ1 peak intensity, quantified from images in F, in Control and VAPA KO leader cells (mean±SEM; Control: n=58 cells; VAPA KO: n=53 cells, from 3 independent experiments). E. Analysis of PM/Cytosol ratio of RFP-PH-PLCδ1, quantified from images in G, at the leading edge of Control and VAPA KO leader cells (mean±SEM; Control: n=27 cells; VAPA KO: n=26 cells, from 3 independent experiments). F. XZ view of confocal images of Control and VAPA KO cells in cluster immunostained for PI(4,5)P2. Scale Bar: 5 µm. G. Confocal images of PI(4,5)P2 distribution in Control and VAPA KO leader cells expressing RFP-PH-PLCδ1, represented as a color-coded heat map. Scale bar: 10 µm. All data were analysed using non parametric Mann-Whitney t-test (ns: non significant, ***P-values <0.001).

    Techniques Used: Expressing, MANN-WHITNEY

    z-p045  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences z-p045
    Z P045, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z-p045/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z-p045 - by Bioz Stars, 2024-04
    93/100 stars

    Images

    mouse monoclonal  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences mouse monoclonal
    Mouse Monoclonal, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal - by Bioz Stars, 2024-04
    93/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Echelon Biosciences z-p045
    Z P045, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z-p045/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z-p045 - by Bioz Stars, 2024-04
    93/100 stars
      Buy from Supplier

    86
    Echelon Biosciences z p045

    Z P045, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z p045/product/Echelon Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z p045 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    Echelon Biosciences pi 4 5 p2 echelon biosciences z p045

    Pi 4 5 P2 Echelon Biosciences Z P045, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi 4 5 p2 echelon biosciences z p045/product/Echelon Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pi 4 5 p2 echelon biosciences z p045 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    93
    Echelon Biosciences pip2
    A , Chemotactic WT or KO MEF ( long arrows : chemotaxis direction) transiently expressing the PIP3 reporter, GFP-PH-AKT, and stained for F-actin, showing enrichment of PIP3 in the leading edge filopodia of KO cells. Short arrows , leading edge lamellipodia (WT cells) or filopodia (KO cells). B , Re-expression of FL-, but not ΔPBD-, SSeCKS-GFP rescues leading edge lamellipodia formation in KO MEF and suppresses leading edge enrichment of the GFP-PH-AKT reporter. Scale bar , 10 µm. C , Quantification of leading edge GFP-PH-AKT levels (normalized to total cell GFP) determined for 3 fields containing at least 10 cells/field in 2 independent experiments. Error bars , S.E. *, p<0.01, **, p<0.005. D , <t>PIP2</t> or PIP3 in chemotactic leading edges of WT or KO MEF by IFA or quantified as in E . **, p<0.01. Scale bar , 10 µm.
    Pip2, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pip2/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pip2 - by Bioz Stars, 2024-04
    93/100 stars
      Buy from Supplier

    93
    Echelon Biosciences mouse anti pi 4 5 p2 monoclonal igm antibody
    A. Confocal images and zoom boxes of PI(4)P distribution in Control and VAPA KO leader cells expressing GFP-PH-OSBP and immunostained for EEA1 or TGN46. Scale Bar: 10 µm. B. Analysis of colocalization by Pearson coefficient determination between GFP-PH-OSBP and EEA1 or TGN46 signals, quantified from images in A, in Control and VAPA KO leader cells (mean±SEM; Control: n=9 cells and 12 cells respectively; VAPA KO: n=9 cells and 12 cells respectively, from 3 independent experiments). C. Analysis of Golgi(TGN46)/cytosol (left) and Endosomes(EEA1)/Cytosol (right) ratio of GFP-PH-OSBP signal, quantified from images in A, in Control and VAPA KO leader cells (mean ±SEM; Control: n=12 cells; VAPA KO: n=11 cells, from 3 independent experiments). D. Analysis of RFP-PH-PLCδ1 peak intensity, quantified from images in F, in Control and VAPA KO leader cells (mean±SEM; Control: n=58 cells; VAPA KO: n=53 cells, from 3 independent experiments). E. Analysis of PM/Cytosol ratio of RFP-PH-PLCδ1, quantified from images in G, at the leading edge of Control and VAPA KO leader cells (mean±SEM; Control: n=27 cells; VAPA KO: n=26 cells, from 3 independent experiments). F. XZ view of confocal images of Control and VAPA KO cells in cluster immunostained for <t>PI(4,5)P2.</t> Scale Bar: 5 µm. G. Confocal images of PI(4,5)P2 distribution in Control and VAPA KO leader cells expressing RFP-PH-PLCδ1, represented as a color-coded heat map. Scale bar: 10 µm. All data were analysed using non parametric Mann-Whitney t-test (ns: non significant, ***P-values <0.001).
    Mouse Anti Pi 4 5 P2 Monoclonal Igm Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti pi 4 5 p2 monoclonal igm antibody/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti pi 4 5 p2 monoclonal igm antibody - by Bioz Stars, 2024-04
    93/100 stars
      Buy from Supplier

    93
    Echelon Biosciences mouse monoclonal
    A. Confocal images and zoom boxes of PI(4)P distribution in Control and VAPA KO leader cells expressing GFP-PH-OSBP and immunostained for EEA1 or TGN46. Scale Bar: 10 µm. B. Analysis of colocalization by Pearson coefficient determination between GFP-PH-OSBP and EEA1 or TGN46 signals, quantified from images in A, in Control and VAPA KO leader cells (mean±SEM; Control: n=9 cells and 12 cells respectively; VAPA KO: n=9 cells and 12 cells respectively, from 3 independent experiments). C. Analysis of Golgi(TGN46)/cytosol (left) and Endosomes(EEA1)/Cytosol (right) ratio of GFP-PH-OSBP signal, quantified from images in A, in Control and VAPA KO leader cells (mean ±SEM; Control: n=12 cells; VAPA KO: n=11 cells, from 3 independent experiments). D. Analysis of RFP-PH-PLCδ1 peak intensity, quantified from images in F, in Control and VAPA KO leader cells (mean±SEM; Control: n=58 cells; VAPA KO: n=53 cells, from 3 independent experiments). E. Analysis of PM/Cytosol ratio of RFP-PH-PLCδ1, quantified from images in G, at the leading edge of Control and VAPA KO leader cells (mean±SEM; Control: n=27 cells; VAPA KO: n=26 cells, from 3 independent experiments). F. XZ view of confocal images of Control and VAPA KO cells in cluster immunostained for <t>PI(4,5)P2.</t> Scale Bar: 5 µm. G. Confocal images of PI(4,5)P2 distribution in Control and VAPA KO leader cells expressing RFP-PH-PLCδ1, represented as a color-coded heat map. Scale bar: 10 µm. All data were analysed using non parametric Mann-Whitney t-test (ns: non significant, ***P-values <0.001).
    Mouse Monoclonal, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal - by Bioz Stars, 2024-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Journal: eLife

    Article Title: Mechanical activation of TWIK-related potassium channel by nanoscopic movement and rapid second messenger signaling

    doi: 10.7554/eLife.89465

    Figure Lengend Snippet:

    Article Snippet: Antibody , Anti-PIP2 (mouse, monoclonal) , Echelon Biosciences , Cat# Z-P045, RRID: AB_427225 , 1:100 dilution.

    Techniques: Transfection, Construct, Recombinant

    A , Chemotactic WT or KO MEF ( long arrows : chemotaxis direction) transiently expressing the PIP3 reporter, GFP-PH-AKT, and stained for F-actin, showing enrichment of PIP3 in the leading edge filopodia of KO cells. Short arrows , leading edge lamellipodia (WT cells) or filopodia (KO cells). B , Re-expression of FL-, but not ΔPBD-, SSeCKS-GFP rescues leading edge lamellipodia formation in KO MEF and suppresses leading edge enrichment of the GFP-PH-AKT reporter. Scale bar , 10 µm. C , Quantification of leading edge GFP-PH-AKT levels (normalized to total cell GFP) determined for 3 fields containing at least 10 cells/field in 2 independent experiments. Error bars , S.E. *, p<0.01, **, p<0.005. D , PIP2 or PIP3 in chemotactic leading edges of WT or KO MEF by IFA or quantified as in E . **, p<0.01. Scale bar , 10 µm.

    Journal: PLoS ONE

    Article Title: Suppression of Chemotaxis by SSeCKS via Scaffolding of Phosphoinositol Phosphates and the Recruitment of the Cdc42 GEF, Frabin, to the Leading Edge

    doi: 10.1371/journal.pone.0111534

    Figure Lengend Snippet: A , Chemotactic WT or KO MEF ( long arrows : chemotaxis direction) transiently expressing the PIP3 reporter, GFP-PH-AKT, and stained for F-actin, showing enrichment of PIP3 in the leading edge filopodia of KO cells. Short arrows , leading edge lamellipodia (WT cells) or filopodia (KO cells). B , Re-expression of FL-, but not ΔPBD-, SSeCKS-GFP rescues leading edge lamellipodia formation in KO MEF and suppresses leading edge enrichment of the GFP-PH-AKT reporter. Scale bar , 10 µm. C , Quantification of leading edge GFP-PH-AKT levels (normalized to total cell GFP) determined for 3 fields containing at least 10 cells/field in 2 independent experiments. Error bars , S.E. *, p<0.01, **, p<0.005. D , PIP2 or PIP3 in chemotactic leading edges of WT or KO MEF by IFA or quantified as in E . **, p<0.01. Scale bar , 10 µm.

    Article Snippet: The primary antibodies (Ab) used include rabbit polyclonal Abs: SSeCKS , GAPDH (sc-25778, Santa Cruz Biotechnology, Santa Cruz, CA), PKC-ζ (sc-216, Santa Cruz), Akt (#9272, Cell Signaling, Beverly, MA); mouse monoclonal Abs: His 6 (G020, Abm, Richmond, BC, Canada), glutathione S-transferase (GST; Abm, G018), Rac1 (ARC03, Cytoskeleton, Denver, CO) Cdc42 (sc-8401, Santa Cruz), PIP3 (Z-P345, Echelon, Salt Lake City, UT), PIP2 (Z-P045, Echelon), Frabin (sc41718, Santa Cruz); goat polyclonal Ab: Par6 (sc-14403, Santa Cruz).

    Techniques: Chemotaxis Assay, Expressing, Staining

    In WT (SSeCKS+/+) cells ( top ), the ability of SSeCKS to scaffold Src at lipid raft sites allows for local activation of PI3K/AKT signaling, thereby producing locally enriched PIP2/3. SSeCKS’ additional ability to scaffold PIPs, including PIP2/3, at the chemotactic leading edge facilitates the local activation of Rac1, resulting in lamellipodial-dependent chemotaxis. In contrast, in the absence of SSeCKS’s scaffolding of PIPs, PIP3 enriches in budding filopodia, which attracts binding of Frabin through its PH and FYVE domains. This leads to local activation of Cdc42 and the subsequent domination of growing filopodia at the leading edge. Of note is that SSeCKS also affects actin cytoskeletal modeling not addressed directly in this paper, such as the generation of thickened, longitudinal stress fibers (SF) and the loss of arc SF in SSeCKS-null cells.

    Journal: PLoS ONE

    Article Title: Suppression of Chemotaxis by SSeCKS via Scaffolding of Phosphoinositol Phosphates and the Recruitment of the Cdc42 GEF, Frabin, to the Leading Edge

    doi: 10.1371/journal.pone.0111534

    Figure Lengend Snippet: In WT (SSeCKS+/+) cells ( top ), the ability of SSeCKS to scaffold Src at lipid raft sites allows for local activation of PI3K/AKT signaling, thereby producing locally enriched PIP2/3. SSeCKS’ additional ability to scaffold PIPs, including PIP2/3, at the chemotactic leading edge facilitates the local activation of Rac1, resulting in lamellipodial-dependent chemotaxis. In contrast, in the absence of SSeCKS’s scaffolding of PIPs, PIP3 enriches in budding filopodia, which attracts binding of Frabin through its PH and FYVE domains. This leads to local activation of Cdc42 and the subsequent domination of growing filopodia at the leading edge. Of note is that SSeCKS also affects actin cytoskeletal modeling not addressed directly in this paper, such as the generation of thickened, longitudinal stress fibers (SF) and the loss of arc SF in SSeCKS-null cells.

    Article Snippet: The primary antibodies (Ab) used include rabbit polyclonal Abs: SSeCKS , GAPDH (sc-25778, Santa Cruz Biotechnology, Santa Cruz, CA), PKC-ζ (sc-216, Santa Cruz), Akt (#9272, Cell Signaling, Beverly, MA); mouse monoclonal Abs: His 6 (G020, Abm, Richmond, BC, Canada), glutathione S-transferase (GST; Abm, G018), Rac1 (ARC03, Cytoskeleton, Denver, CO) Cdc42 (sc-8401, Santa Cruz), PIP3 (Z-P345, Echelon, Salt Lake City, UT), PIP2 (Z-P045, Echelon), Frabin (sc41718, Santa Cruz); goat polyclonal Ab: Par6 (sc-14403, Santa Cruz).

    Techniques: Activation Assay, Chemotaxis Assay, Scaffolding, Binding Assay

    A. Confocal images and zoom boxes of PI(4)P distribution in Control and VAPA KO leader cells expressing GFP-PH-OSBP and immunostained for EEA1 or TGN46. Scale Bar: 10 µm. B. Analysis of colocalization by Pearson coefficient determination between GFP-PH-OSBP and EEA1 or TGN46 signals, quantified from images in A, in Control and VAPA KO leader cells (mean±SEM; Control: n=9 cells and 12 cells respectively; VAPA KO: n=9 cells and 12 cells respectively, from 3 independent experiments). C. Analysis of Golgi(TGN46)/cytosol (left) and Endosomes(EEA1)/Cytosol (right) ratio of GFP-PH-OSBP signal, quantified from images in A, in Control and VAPA KO leader cells (mean ±SEM; Control: n=12 cells; VAPA KO: n=11 cells, from 3 independent experiments). D. Analysis of RFP-PH-PLCδ1 peak intensity, quantified from images in F, in Control and VAPA KO leader cells (mean±SEM; Control: n=58 cells; VAPA KO: n=53 cells, from 3 independent experiments). E. Analysis of PM/Cytosol ratio of RFP-PH-PLCδ1, quantified from images in G, at the leading edge of Control and VAPA KO leader cells (mean±SEM; Control: n=27 cells; VAPA KO: n=26 cells, from 3 independent experiments). F. XZ view of confocal images of Control and VAPA KO cells in cluster immunostained for PI(4,5)P2. Scale Bar: 5 µm. G. Confocal images of PI(4,5)P2 distribution in Control and VAPA KO leader cells expressing RFP-PH-PLCδ1, represented as a color-coded heat map. Scale bar: 10 µm. All data were analysed using non parametric Mann-Whitney t-test (ns: non significant, ***P-values <0.001).

    Journal: bioRxiv

    Article Title: The ER tether VAPA is required for proper cell motility and for anchoring ER-plasma membrane contact sites to focal adhesions

    doi: 10.1101/2022.10.17.512434

    Figure Lengend Snippet: A. Confocal images and zoom boxes of PI(4)P distribution in Control and VAPA KO leader cells expressing GFP-PH-OSBP and immunostained for EEA1 or TGN46. Scale Bar: 10 µm. B. Analysis of colocalization by Pearson coefficient determination between GFP-PH-OSBP and EEA1 or TGN46 signals, quantified from images in A, in Control and VAPA KO leader cells (mean±SEM; Control: n=9 cells and 12 cells respectively; VAPA KO: n=9 cells and 12 cells respectively, from 3 independent experiments). C. Analysis of Golgi(TGN46)/cytosol (left) and Endosomes(EEA1)/Cytosol (right) ratio of GFP-PH-OSBP signal, quantified from images in A, in Control and VAPA KO leader cells (mean ±SEM; Control: n=12 cells; VAPA KO: n=11 cells, from 3 independent experiments). D. Analysis of RFP-PH-PLCδ1 peak intensity, quantified from images in F, in Control and VAPA KO leader cells (mean±SEM; Control: n=58 cells; VAPA KO: n=53 cells, from 3 independent experiments). E. Analysis of PM/Cytosol ratio of RFP-PH-PLCδ1, quantified from images in G, at the leading edge of Control and VAPA KO leader cells (mean±SEM; Control: n=27 cells; VAPA KO: n=26 cells, from 3 independent experiments). F. XZ view of confocal images of Control and VAPA KO cells in cluster immunostained for PI(4,5)P2. Scale Bar: 5 µm. G. Confocal images of PI(4,5)P2 distribution in Control and VAPA KO leader cells expressing RFP-PH-PLCδ1, represented as a color-coded heat map. Scale bar: 10 µm. All data were analysed using non parametric Mann-Whitney t-test (ns: non significant, ***P-values <0.001).

    Article Snippet: The following primary antibodies were used: mouse anti-EEA1 monoclonal antibody (BD transduction, 610456), rabbit anti-VAPB polyclonal antibody (Atlas antibody HPA 013144), mouse anti-VAPA monoclonal antibody (Sigma 10355-4C12) and rabbit anti-VAPA polyclonal antibody for the immunoblot (Sigma SAB2702059), rabbit anti-TGN46 polyclonal antibody (abcam ab50595), mouse anti-paxilline monoclonal antibody (Merck, 05-417), mouse anti-cortactin monoclonal antibody (Merck, 05-180), mouse anti-tubulin monoclonal antibody (GeneTex GTX628802), mouse anti-PI(4,5)P2 monoclonal IgM antibody (Echelon Z-P045).

    Techniques: Expressing, MANN-WHITNEY