z taq polymerase  (TaKaRa)

 
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    Name:
    TaKaRa Z Taq DNA Polymerase
    Description:
    TaKaRa Z Taq DNA Polymerase enables rapid PCR amplification due to its greater processivity relative to standard Taq DNA polymerase Z Taq polymerase can amplify a 1 kb DNA fragment from genomic DNA in 20 minutes using a 30 cycle two step amplification protocol The improved processivity of the Z Taq enzyme also enables the amplification of longer fragments than standard Taq DNA polymerase up to 20 kb from lambda DNA This kit includes separate tubes of enzyme buffer and dNTPs
    Catalog Number:
    r006b
    Price:
    None
    Size:
    800 Units
    Category:
    Z Taq DNA polymerase Fast PCR PCR
    Buy from Supplier


    Structured Review

    TaKaRa z taq polymerase
    TaKaRa Z Taq DNA Polymerase enables rapid PCR amplification due to its greater processivity relative to standard Taq DNA polymerase Z Taq polymerase can amplify a 1 kb DNA fragment from genomic DNA in 20 minutes using a 30 cycle two step amplification protocol The improved processivity of the Z Taq enzyme also enables the amplification of longer fragments than standard Taq DNA polymerase up to 20 kb from lambda DNA This kit includes separate tubes of enzyme buffer and dNTPs
    https://www.bioz.com/result/z taq polymerase/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z taq polymerase - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Restriction Site-dependent PCR: An Efficient Technique for Fast Cloning of New Genes of Microorganisms
    Article Snippet: The same fragments were amplified even after the annealing and the elongation temperatures were lowed down to 38°C and 60°C using Z Taq -DNA polymerase (Takara) (data not shown). .. The parameters were further optimized with cloning of the published ak from T. maritima as an example.

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The cDNA of maH7N7 was sequenced by direct dideoxy-terminated cycle sequencing using BigDye terminator v1.1 sequencing kit (Applied Biosystems) and a 3130 genetic analyzer sequencer (Applied Biosystems). cDNAs of chH7N7 virus were produced by PCR, isolated and cloned into the pGEM-T vector (Promega).

    Amplification:

    Article Title: Rapid Identification of Gram-Negative Bacteria with and without CTX-M Extended-Spectrum ?-Lactamase from Positive Blood Culture Bottles by PCR Followed by Microchip Gel Electrophoresis ▿
    Article Snippet: PCR was performed with 1.5 μl of the template DNA in a total reaction volume of 30 μl consisting of 0.75 U of Z- Taq DNA polymerase (Takara Bio Inc., Shiga, Japan), 0.1 μM each primer, 0.2 mM each deoxynucleoside triphosphate, and 3 mM MgCl2 . .. The ESBL genes were also amplified by PCR.

    Article Title: Restriction Site-dependent PCR: An Efficient Technique for Fast Cloning of New Genes of Microorganisms
    Article Snippet: .. The same fragments were amplified even after the annealing and the elongation temperatures were lowed down to 38°C and 60°C using Z Taq -DNA polymerase (Takara) (data not shown). ..

    Article Title: Antagonistic effects of exogenous Slit2 on VEGF-induced choroidal endothelial cell migration and tube formation
    Article Snippet: .. The mRNA expression levels of Slit2, Robo1, Robo4 and β-actin were assessed via PCR amplification of target cDNA sequences using TaKaRa Z-Taq™ DNA Polymerase (Takara Bio, Inc.). .. The thermocycling conditions were as follows: 94°C for 5 min followed by 30 cycles of 94°C for 30 sec, 60°C for 30 sec, 72°C for 60 sec, and maintenance at 72°C for 10 min. PCR products were resolved by 2% agarose gel electrophoresis, and visualized by staining with ethidium bromide.

    Article Title: Disordered Cell Integrity Signaling Caused by Disruption of the kexB Gene in Aspergillus oryzae †
    Article Snippet: .. The kexB gene was PCR amplified using Z-Taq DNA polymerase (TaKaRa) and a pair of primers, 5′- AAGCTT ATAATGCGGCTTTCCGAAAG-3′ and 5′- AAGCTT GTAGAAGCAAATGCAAAGCC-3′. .. Each primer was designed to introduce a HindIII site (underlined).

    Article Title: Reassortments among Avian Influenza A(H5N1) Viruses Circulating in Indonesia, 2015–2016
    Article Snippet: .. We performed amplification of the full genomes of HPAI H5N1 viruses using a 2-step RT-PCR TaKaRa Z-Taq DNA Polymerase (Takara Bio, http://www.takarabio.com ) or Toyobo KOD FX Neo (Toyobo, http://www.toyobo-global.com ) if the genomes were not successfully amplified using the Takara product. ..

    Article Title: Physical Map Location of the Multicopy Genes Coding for Ammonia Monooxygenase and Hydroxylamine Oxidoreductase in the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain ENI-11
    Article Snippet: The amoB probe was amplified by PCR with primers A3 (5′-TATGTACTGCAGGCAGAAGTTGCGCTTG) and A4 (5′-CGAATTCGACAGGCTAATTGATGCTTCG) from the ENI-11 genome. .. PCR was performed with respective sets of oligonucleotide primers and a Takara Z Taq DNA polymerase (Takara Shuzo Co., Shiga, Japan) on a DNA thermal cycler (Perkin-Elmer).

    Article Title: Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
    Article Snippet: .. Prohibitin cDNA was amplified by Z-Taq DNA polymerase (TaKaRa, Otsu, Japan) with prohibitin-specific sense (5′-CAGAGTGGAAGCAGGTGTGAG-3′) and antisense adaptor-containing (5′-GGCCACGCGTCCGACTAGTAC-3′) primers. ..

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: .. RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The PCR products were excised from an agarose gel and then purified using a Zymoclean kit (Zymo Research) as indicated by the manufacturer.

    Article Title: First Complete Genome Sequences of Genogroup VI Porcine Sapoviruses
    Article Snippet: .. The upstream region (1.5 kb) of JJ681 was amplified by nested reverse transcription (RT)-PCR using random hexamers (Invitrogen) and gene-specific reverse primers with Z-Taq DNA polymerase (TaKaRa) and GoTaq DNA polymerase (Promega). .. Then, the region further upstream (1.5 kb) was amplified using a forward primer, designed based on an SaV conserved motif corresponding to nucleotides (nt) 1,408 to 1,421 of the Po/SaV/GIII/Cowden strain (GenBank accession no. AF182760) ( ) and a specific reverse primer using EX-Taq DNA polymerase (TaKaRa).

    Article Title: Multidrug-Resistant Pseudomonas aeruginosa Strain That Caused an Outbreak in a Neurosurgery Ward and Its aac(6′)-Iae Gene Cassette Encoding a Novel Aminoglycoside Acetyltransferase
    Article Snippet: .. Amplification conditions were 30 cycles of 94°C for 1 min, 50°C for 1 min, and 72°C for 3 min or 5 min. PCR for amplicons longer than 1 kb was performed with 1.25 U of Z - Taq polymerase (Takara Bio) and 30 cycles of 95°C for 1 s and 68°C for 120 s according to the manufacturer's instructions. .. The gyrA , gyrB , parC , and parE quinolone resistance-determining regions (QRDRs) of P. aeruginosa were amplified by PCR with the primers listed in Table according to methods described previously ( , , , ).

    Article Title: Comparison of Genome Sequences of Single-Stranded RNA Viruses Infecting the Bivalve-Killing Dinoflagellate Heterocapsa circularisquama
    Article Snippet: .. The second PCR amplification was performed using a 50-μl mixture containing 1.2 μl of diluted product from the first PCR, 1× Z- Taq buffer (Takara Bio Inc.), each deoxynucleoside triphosphate at a concentration of 200 μM, 4 pmol of primers MCPF2 and MCPR1, and 1.25 U of Z- Taq DNA polymerase (Takara Bio Inc.) and the GeneAmp PCR System 9700 (Applied Biosystems); it consisted of 35 cycles of denaturation at 98°C for 1 s, annealing at 58°C for 2 s, and extension at 72°C for 10 s. The resultant products were electrophoresed in 2% (wt/vol) agarose S gels (Nippon Gene Co., Ltd.). .. The nucleic acids were visualized by ethidium bromide staining.

    Synthesized:

    Article Title: Reassortments among Avian Influenza A(H5N1) Viruses Circulating in Indonesia, 2015–2016
    Article Snippet: Sequencing At the Eijkman Institute, we rescreened the specimens and extracted RNA in accordance with the protocol of the manufacturer and synthesized cDNA by Invitrogen Super Script III First-Strand Synthesis SuperMix (Thermo Fisher Scientific, http://www.thermofisher.com ) with Uni12 primer ( ). .. We performed amplification of the full genomes of HPAI H5N1 viruses using a 2-step RT-PCR TaKaRa Z-Taq DNA Polymerase (Takara Bio, http://www.takarabio.com ) or Toyobo KOD FX Neo (Toyobo, http://www.toyobo-global.com ) if the genomes were not successfully amplified using the Takara product.

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: .. RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The PCR products were excised from an agarose gel and then purified using a Zymoclean kit (Zymo Research) as indicated by the manufacturer.

    TA Cloning:

    Article Title: Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
    Article Snippet: Prohibitin cDNA was amplified by Z-Taq DNA polymerase (TaKaRa, Otsu, Japan) with prohibitin-specific sense (5′-CAGAGTGGAAGCAGGTGTGAG-3′) and antisense adaptor-containing (5′-GGCCACGCGTCCGACTAGTAC-3′) primers. .. The DNA fragment was subcloned into a pCR2.1 plasmid vector using a TA cloning kit (Invitrogen).

    Construct:

    Article Title: Disordered Cell Integrity Signaling Caused by Disruption of the kexB Gene in Aspergillus oryzae †
    Article Snippet: The overexpression plasmid pNAKX1 was constructed as follows. .. The kexB gene was PCR amplified using Z-Taq DNA polymerase (TaKaRa) and a pair of primers, 5′- AAGCTT ATAATGCGGCTTTCCGAAAG-3′ and 5′- AAGCTT GTAGAAGCAAATGCAAAGCC-3′.

    Article Title: Antagonistic Regulation of Cell-Matrix Adhesion by FosB and ?FosB/?2?FosB Encoded by Alternatively Spliced Forms of fosB Transcripts
    Article Snippet: A 19-kb genomic fragment containing an entire sequence of the fosB gene was isolated from a 129/Sv mouse genomic library (Stratagene, La Jolla, CA) to generate two types of fosB -targeting constructs. .. The second PCR was performed with primer FB10 and with the resulting PCR product as a Mega-primer and using the same template as with the first PCR using zTaq DNA polymerase (Takara Bio).

    Incubation:

    Article Title: Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
    Article Snippet: Ten units of RNase H (Toyobo, Osaka, Japan) were added, and the mixture was incubated at 37°C for 20 min. .. Prohibitin cDNA was amplified by Z-Taq DNA polymerase (TaKaRa, Otsu, Japan) with prohibitin-specific sense (5′-CAGAGTGGAAGCAGGTGTGAG-3′) and antisense adaptor-containing (5′-GGCCACGCGTCCGACTAGTAC-3′) primers.

    Infection:

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: .. RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The PCR products were excised from an agarose gel and then purified using a Zymoclean kit (Zymo Research) as indicated by the manufacturer.

    Expressing:

    Article Title: Antagonistic effects of exogenous Slit2 on VEGF-induced choroidal endothelial cell migration and tube formation
    Article Snippet: .. The mRNA expression levels of Slit2, Robo1, Robo4 and β-actin were assessed via PCR amplification of target cDNA sequences using TaKaRa Z-Taq™ DNA Polymerase (Takara Bio, Inc.). .. The thermocycling conditions were as follows: 94°C for 5 min followed by 30 cycles of 94°C for 30 sec, 60°C for 30 sec, 72°C for 60 sec, and maintenance at 72°C for 10 min. PCR products were resolved by 2% agarose gel electrophoresis, and visualized by staining with ethidium bromide.

    Over Expression:

    Article Title: Disordered Cell Integrity Signaling Caused by Disruption of the kexB Gene in Aspergillus oryzae †
    Article Snippet: The overexpression plasmid pNAKX1 was constructed as follows. .. The kexB gene was PCR amplified using Z-Taq DNA polymerase (TaKaRa) and a pair of primers, 5′- AAGCTT ATAATGCGGCTTTCCGAAAG-3′ and 5′- AAGCTT GTAGAAGCAAATGCAAAGCC-3′.

    Introduce:

    Article Title: Disordered Cell Integrity Signaling Caused by Disruption of the kexB Gene in Aspergillus oryzae †
    Article Snippet: The kexB gene was PCR amplified using Z-Taq DNA polymerase (TaKaRa) and a pair of primers, 5′- AAGCTT ATAATGCGGCTTTCCGAAAG-3′ and 5′- AAGCTT GTAGAAGCAAATGCAAAGCC-3′. .. Each primer was designed to introduce a HindIII site (underlined).

    Polymerase Chain Reaction:

    Article Title: Rapid Identification of Gram-Negative Bacteria with and without CTX-M Extended-Spectrum ?-Lactamase from Positive Blood Culture Bottles by PCR Followed by Microchip Gel Electrophoresis ▿
    Article Snippet: .. PCR was performed with 1.5 μl of the template DNA in a total reaction volume of 30 μl consisting of 0.75 U of Z- Taq DNA polymerase (Takara Bio Inc., Shiga, Japan), 0.1 μM each primer, 0.2 mM each deoxynucleoside triphosphate, and 3 mM MgCl2 . .. Reactions were run in a thermocycler (Biometra Co., Göttingen, Germany).

    Article Title: Antagonistic effects of exogenous Slit2 on VEGF-induced choroidal endothelial cell migration and tube formation
    Article Snippet: .. The mRNA expression levels of Slit2, Robo1, Robo4 and β-actin were assessed via PCR amplification of target cDNA sequences using TaKaRa Z-Taq™ DNA Polymerase (Takara Bio, Inc.). .. The thermocycling conditions were as follows: 94°C for 5 min followed by 30 cycles of 94°C for 30 sec, 60°C for 30 sec, 72°C for 60 sec, and maintenance at 72°C for 10 min. PCR products were resolved by 2% agarose gel electrophoresis, and visualized by staining with ethidium bromide.

    Article Title: Disordered Cell Integrity Signaling Caused by Disruption of the kexB Gene in Aspergillus oryzae †
    Article Snippet: .. The kexB gene was PCR amplified using Z-Taq DNA polymerase (TaKaRa) and a pair of primers, 5′- AAGCTT ATAATGCGGCTTTCCGAAAG-3′ and 5′- AAGCTT GTAGAAGCAAATGCAAAGCC-3′. .. Each primer was designed to introduce a HindIII site (underlined).

    Article Title: Reassortments among Avian Influenza A(H5N1) Viruses Circulating in Indonesia, 2015–2016
    Article Snippet: On specimens that tested positive in this PCR, we performed additional PCRs to amplify other gene segments present in the samples. .. We performed amplification of the full genomes of HPAI H5N1 viruses using a 2-step RT-PCR TaKaRa Z-Taq DNA Polymerase (Takara Bio, http://www.takarabio.com ) or Toyobo KOD FX Neo (Toyobo, http://www.toyobo-global.com ) if the genomes were not successfully amplified using the Takara product.

    Article Title: Physical Map Location of the Multicopy Genes Coding for Ammonia Monooxygenase and Hydroxylamine Oxidoreductase in the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain ENI-11
    Article Snippet: .. PCR was performed with respective sets of oligonucleotide primers and a Takara Z Taq DNA polymerase (Takara Shuzo Co., Shiga, Japan) on a DNA thermal cycler (Perkin-Elmer). .. The 2.3-kb PCR product was digested with Bam HI and Sph I, and the resulting 0.3-kb fragment was labeled by a nonisotopic method (fluorescein DNA labeling and detection kit; Amersham).

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: .. RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The PCR products were excised from an agarose gel and then purified using a Zymoclean kit (Zymo Research) as indicated by the manufacturer.

    Article Title: Multidrug-Resistant Pseudomonas aeruginosa Strain That Caused an Outbreak in a Neurosurgery Ward and Its aac(6′)-Iae Gene Cassette Encoding a Novel Aminoglycoside Acetyltransferase
    Article Snippet: .. Amplification conditions were 30 cycles of 94°C for 1 min, 50°C for 1 min, and 72°C for 3 min or 5 min. PCR for amplicons longer than 1 kb was performed with 1.25 U of Z - Taq polymerase (Takara Bio) and 30 cycles of 95°C for 1 s and 68°C for 120 s according to the manufacturer's instructions. .. The gyrA , gyrB , parC , and parE quinolone resistance-determining regions (QRDRs) of P. aeruginosa were amplified by PCR with the primers listed in Table according to methods described previously ( , , , ).

    Article Title: Antagonistic Regulation of Cell-Matrix Adhesion by FosB and ?FosB/?2?FosB Encoded by Alternatively Spliced Forms of fosB Transcripts
    Article Snippet: .. The second PCR was performed with primer FB10 and with the resulting PCR product as a Mega-primer and using the same template as with the first PCR using zTaq DNA polymerase (Takara Bio). .. Next, the AatII-SgrAI fragment, excised from the PCR product, was reciprocally exchanged with the corresponding region surrounding exon 4 of the targeting vector.

    Article Title: Comparison of Genome Sequences of Single-Stranded RNA Viruses Infecting the Bivalve-Killing Dinoflagellate Heterocapsa circularisquama
    Article Snippet: .. The second PCR amplification was performed using a 50-μl mixture containing 1.2 μl of diluted product from the first PCR, 1× Z- Taq buffer (Takara Bio Inc.), each deoxynucleoside triphosphate at a concentration of 200 μM, 4 pmol of primers MCPF2 and MCPR1, and 1.25 U of Z- Taq DNA polymerase (Takara Bio Inc.) and the GeneAmp PCR System 9700 (Applied Biosystems); it consisted of 35 cycles of denaturation at 98°C for 1 s, annealing at 58°C for 2 s, and extension at 72°C for 10 s. The resultant products were electrophoresed in 2% (wt/vol) agarose S gels (Nippon Gene Co., Ltd.). .. The nucleic acids were visualized by ethidium bromide staining.

    DNA Sequencing:

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: .. RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The PCR products were excised from an agarose gel and then purified using a Zymoclean kit (Zymo Research) as indicated by the manufacturer.

    DNA Labeling:

    Article Title: Physical Map Location of the Multicopy Genes Coding for Ammonia Monooxygenase and Hydroxylamine Oxidoreductase in the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain ENI-11
    Article Snippet: PCR was performed with respective sets of oligonucleotide primers and a Takara Z Taq DNA polymerase (Takara Shuzo Co., Shiga, Japan) on a DNA thermal cycler (Perkin-Elmer). .. The 2.3-kb PCR product was digested with Bam HI and Sph I, and the resulting 0.3-kb fragment was labeled by a nonisotopic method (fluorescein DNA labeling and detection kit; Amersham).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Antagonistic effects of exogenous Slit2 on VEGF-induced choroidal endothelial cell migration and tube formation
    Article Snippet: Paragraph title: RT-PCR ... The mRNA expression levels of Slit2, Robo1, Robo4 and β-actin were assessed via PCR amplification of target cDNA sequences using TaKaRa Z-Taq™ DNA Polymerase (Takara Bio, Inc.).

    Article Title: Reassortments among Avian Influenza A(H5N1) Viruses Circulating in Indonesia, 2015–2016
    Article Snippet: .. We performed amplification of the full genomes of HPAI H5N1 viruses using a 2-step RT-PCR TaKaRa Z-Taq DNA Polymerase (Takara Bio, http://www.takarabio.com ) or Toyobo KOD FX Neo (Toyobo, http://www.toyobo-global.com ) if the genomes were not successfully amplified using the Takara product. ..

    Article Title: Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
    Article Snippet: Canine prohibitin cDNA was amplified by RT-PCR. .. Prohibitin cDNA was amplified by Z-Taq DNA polymerase (TaKaRa, Otsu, Japan) with prohibitin-specific sense (5′-CAGAGTGGAAGCAGGTGTGAG-3′) and antisense adaptor-containing (5′-GGCCACGCGTCCGACTAGTAC-3′) primers.

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: .. RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The PCR products were excised from an agarose gel and then purified using a Zymoclean kit (Zymo Research) as indicated by the manufacturer.

    Article Title: First Complete Genome Sequences of Genogroup VI Porcine Sapoviruses
    Article Snippet: .. The upstream region (1.5 kb) of JJ681 was amplified by nested reverse transcription (RT)-PCR using random hexamers (Invitrogen) and gene-specific reverse primers with Z-Taq DNA polymerase (TaKaRa) and GoTaq DNA polymerase (Promega). .. Then, the region further upstream (1.5 kb) was amplified using a forward primer, designed based on an SaV conserved motif corresponding to nucleotides (nt) 1,408 to 1,421 of the Po/SaV/GIII/Cowden strain (GenBank accession no. AF182760) ( ) and a specific reverse primer using EX-Taq DNA polymerase (TaKaRa).

    Mutagenesis:

    Article Title: Antagonistic Regulation of Cell-Matrix Adhesion by FosB and ?FosB/?2?FosB Encoded by Alternatively Spliced Forms of fosB Transcripts
    Article Snippet: These also function as an alternative splicing donor site for the exonic intron in exon 4 of the fosB gene to produce ΔFosB mRNA and were replaced by two tandem stop codons (TAG, TGA) by PCR-mediated site-directed mutagenesis using a Mega-primer protocol (see B; ). .. The second PCR was performed with primer FB10 and with the resulting PCR product as a Mega-primer and using the same template as with the first PCR using zTaq DNA polymerase (Takara Bio).

    Isolation:

    Article Title: Antagonistic effects of exogenous Slit2 on VEGF-induced choroidal endothelial cell migration and tube formation
    Article Snippet: RT-PCR Total RNA from CECs was isolated using TRIzol reagent (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. .. The mRNA expression levels of Slit2, Robo1, Robo4 and β-actin were assessed via PCR amplification of target cDNA sequences using TaKaRa Z-Taq™ DNA Polymerase (Takara Bio, Inc.).

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: .. RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The PCR products were excised from an agarose gel and then purified using a Zymoclean kit (Zymo Research) as indicated by the manufacturer.

    Article Title: First Complete Genome Sequences of Genogroup VI Porcine Sapoviruses
    Article Snippet: We determined the full-length genome sequences of two GVI SaVs, Po/SaV/GVI/OH-JJ681/2000/US (JJ681) and Po/SaV/GVI/OH-JJ674/2000/US (JJ674), isolated from feces from two diarrheic postweaning pigs from the same Ohio farm on 22 December 2000 ( , ). .. The upstream region (1.5 kb) of JJ681 was amplified by nested reverse transcription (RT)-PCR using random hexamers (Invitrogen) and gene-specific reverse primers with Z-Taq DNA polymerase (TaKaRa) and GoTaq DNA polymerase (Promega).

    Article Title: Antagonistic Regulation of Cell-Matrix Adhesion by FosB and ?FosB/?2?FosB Encoded by Alternatively Spliced Forms of fosB Transcripts
    Article Snippet: A 19-kb genomic fragment containing an entire sequence of the fosB gene was isolated from a 129/Sv mouse genomic library (Stratagene, La Jolla, CA) to generate two types of fosB -targeting constructs. .. The second PCR was performed with primer FB10 and with the resulting PCR product as a Mega-primer and using the same template as with the first PCR using zTaq DNA polymerase (Takara Bio).

    Size-exclusion Chromatography:

    Article Title: Antagonistic effects of exogenous Slit2 on VEGF-induced choroidal endothelial cell migration and tube formation
    Article Snippet: The mRNA expression levels of Slit2, Robo1, Robo4 and β-actin were assessed via PCR amplification of target cDNA sequences using TaKaRa Z-Taq™ DNA Polymerase (Takara Bio, Inc.). .. The thermocycling conditions were as follows: 94°C for 5 min followed by 30 cycles of 94°C for 30 sec, 60°C for 30 sec, 72°C for 60 sec, and maintenance at 72°C for 10 min. PCR products were resolved by 2% agarose gel electrophoresis, and visualized by staining with ethidium bromide.

    Labeling:

    Article Title: Physical Map Location of the Multicopy Genes Coding for Ammonia Monooxygenase and Hydroxylamine Oxidoreductase in the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain ENI-11
    Article Snippet: PCR was performed with respective sets of oligonucleotide primers and a Takara Z Taq DNA polymerase (Takara Shuzo Co., Shiga, Japan) on a DNA thermal cycler (Perkin-Elmer). .. The 2.3-kb PCR product was digested with Bam HI and Sph I, and the resulting 0.3-kb fragment was labeled by a nonisotopic method (fluorescein DNA labeling and detection kit; Amersham).

    Purification:

    Article Title: Reassortments among Avian Influenza A(H5N1) Viruses Circulating in Indonesia, 2015–2016
    Article Snippet: We performed amplification of the full genomes of HPAI H5N1 viruses using a 2-step RT-PCR TaKaRa Z-Taq DNA Polymerase (Takara Bio, http://www.takarabio.com ) or Toyobo KOD FX Neo (Toyobo, http://www.toyobo-global.com ) if the genomes were not successfully amplified using the Takara product. .. We purified the amplified PCR products with Roche High Pure PCR Product Purification Kit (Roche) or Zymoclean Gel DNA Recovery Kit (Zymo Research, https://www.zymoresearch.com ) for PCR products for which gel separation was necessary, and subsequently sequenced them using a BigDye Terminator v3.1 Cycle Sequencing Kit in an ABI 3130 Genetic Analyzer (both from Thermo Fisher).

    Article Title: Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
    Article Snippet: Prohibitin cDNA was amplified by Z-Taq DNA polymerase (TaKaRa, Otsu, Japan) with prohibitin-specific sense (5′-CAGAGTGGAAGCAGGTGTGAG-3′) and antisense adaptor-containing (5′-GGCCACGCGTCCGACTAGTAC-3′) primers. .. The PCR product was detected using 1.0% agarose gel electrophoresis and purified using a GFXTM column (GE Healthcare Bioscience, Piscataway, NJ, U.S.A.).

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The PCR products were excised from an agarose gel and then purified using a Zymoclean kit (Zymo Research) as indicated by the manufacturer.

    Article Title: Comparison of Genome Sequences of Single-Stranded RNA Viruses Infecting the Bivalve-Killing Dinoflagellate Heterocapsa circularisquama
    Article Snippet: The second PCR amplification was performed using a 50-μl mixture containing 1.2 μl of diluted product from the first PCR, 1× Z- Taq buffer (Takara Bio Inc.), each deoxynucleoside triphosphate at a concentration of 200 μM, 4 pmol of primers MCPF2 and MCPR1, and 1.25 U of Z- Taq DNA polymerase (Takara Bio Inc.) and the GeneAmp PCR System 9700 (Applied Biosystems); it consisted of 35 cycles of denaturation at 98°C for 1 s, annealing at 58°C for 2 s, and extension at 72°C for 10 s. The resultant products were electrophoresed in 2% (wt/vol) agarose S gels (Nippon Gene Co., Ltd.). .. The bands were excised from the gel and purified using GenElute agarose spin columns (Supelco, Inc.) according to the manufacturer's recommendations.

    Sequencing:

    Article Title: Reassortments among Avian Influenza A(H5N1) Viruses Circulating in Indonesia, 2015–2016
    Article Snippet: Paragraph title: Sequencing ... We performed amplification of the full genomes of HPAI H5N1 viruses using a 2-step RT-PCR TaKaRa Z-Taq DNA Polymerase (Takara Bio, http://www.takarabio.com ) or Toyobo KOD FX Neo (Toyobo, http://www.toyobo-global.com ) if the genomes were not successfully amplified using the Takara product.

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: .. RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The PCR products were excised from an agarose gel and then purified using a Zymoclean kit (Zymo Research) as indicated by the manufacturer.

    Article Title: First Complete Genome Sequences of Genogroup VI Porcine Sapoviruses
    Article Snippet: The 3′-end 3-kb sequence of JJ681 has been reported previously ( ). .. The upstream region (1.5 kb) of JJ681 was amplified by nested reverse transcription (RT)-PCR using random hexamers (Invitrogen) and gene-specific reverse primers with Z-Taq DNA polymerase (TaKaRa) and GoTaq DNA polymerase (Promega).

    Article Title: Antagonistic Regulation of Cell-Matrix Adhesion by FosB and ?FosB/?2?FosB Encoded by Alternatively Spliced Forms of fosB Transcripts
    Article Snippet: A 19-kb genomic fragment containing an entire sequence of the fosB gene was isolated from a 129/Sv mouse genomic library (Stratagene, La Jolla, CA) to generate two types of fosB -targeting constructs. .. The second PCR was performed with primer FB10 and with the resulting PCR product as a Mega-primer and using the same template as with the first PCR using zTaq DNA polymerase (Takara Bio).

    Nested PCR:

    Article Title: First Complete Genome Sequences of Genogroup VI Porcine Sapoviruses
    Article Snippet: The upstream region (1.5 kb) of JJ681 was amplified by nested reverse transcription (RT)-PCR using random hexamers (Invitrogen) and gene-specific reverse primers with Z-Taq DNA polymerase (TaKaRa) and GoTaq DNA polymerase (Promega). .. Briefly, homopolymeric (dC or dA) tailing and nested PCR were performed with gene-specific primers and the abridged anchor primer (AAP) (5′-GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG-3′) and the abridged universal primer (AUAP) (5′-GGCCACGCGTCGACTAGTAC-3′) (Invitrogen) for poly(C)-tailed cDNA and primers QT (5′-CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGCTTTTTTTTTTTTTTTTT-3′) ( ) and QO (5′-CCAGTGAGCAGAGTGACG-3′) ( ) for poly(A)-tailed cDNA.

    Plasmid Preparation:

    Article Title: Disordered Cell Integrity Signaling Caused by Disruption of the kexB Gene in Aspergillus oryzae †
    Article Snippet: The overexpression plasmid pNAKX1 was constructed as follows. .. The kexB gene was PCR amplified using Z-Taq DNA polymerase (TaKaRa) and a pair of primers, 5′- AAGCTT ATAATGCGGCTTTCCGAAAG-3′ and 5′- AAGCTT GTAGAAGCAAATGCAAAGCC-3′.

    Article Title: Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
    Article Snippet: Prohibitin cDNA was amplified by Z-Taq DNA polymerase (TaKaRa, Otsu, Japan) with prohibitin-specific sense (5′-CAGAGTGGAAGCAGGTGTGAG-3′) and antisense adaptor-containing (5′-GGCCACGCGTCCGACTAGTAC-3′) primers. .. The DNA fragment was subcloned into a pCR2.1 plasmid vector using a TA cloning kit (Invitrogen).

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The cDNA of maH7N7 was sequenced by direct dideoxy-terminated cycle sequencing using BigDye terminator v1.1 sequencing kit (Applied Biosystems) and a 3130 genetic analyzer sequencer (Applied Biosystems). cDNAs of chH7N7 virus were produced by PCR, isolated and cloned into the pGEM-T vector (Promega).

    Article Title: Antagonistic Regulation of Cell-Matrix Adhesion by FosB and ?FosB/?2?FosB Encoded by Alternatively Spliced Forms of fosB Transcripts
    Article Snippet: In the pTV fosBdN vector, a pol II- neo -poly(A) cassette flanked by two loxP sites was placed at the ScaI site of intron 3 in opposite orientation to the fosB gene. .. The second PCR was performed with primer FB10 and with the resulting PCR product as a Mega-primer and using the same template as with the first PCR using zTaq DNA polymerase (Takara Bio).

    Article Title: Comparison of Genome Sequences of Single-Stranded RNA Viruses Infecting the Bivalve-Killing Dinoflagellate Heterocapsa circularisquama
    Article Snippet: The second PCR amplification was performed using a 50-μl mixture containing 1.2 μl of diluted product from the first PCR, 1× Z- Taq buffer (Takara Bio Inc.), each deoxynucleoside triphosphate at a concentration of 200 μM, 4 pmol of primers MCPF2 and MCPR1, and 1.25 U of Z- Taq DNA polymerase (Takara Bio Inc.) and the GeneAmp PCR System 9700 (Applied Biosystems); it consisted of 35 cycles of denaturation at 98°C for 1 s, annealing at 58°C for 2 s, and extension at 72°C for 10 s. The resultant products were electrophoresed in 2% (wt/vol) agarose S gels (Nippon Gene Co., Ltd.). .. The resultant PCR products were ligated into the pGEM-T Easy vector (Promega).

    Agarose Gel Electrophoresis:

    Article Title: Antagonistic effects of exogenous Slit2 on VEGF-induced choroidal endothelial cell migration and tube formation
    Article Snippet: The mRNA expression levels of Slit2, Robo1, Robo4 and β-actin were assessed via PCR amplification of target cDNA sequences using TaKaRa Z-Taq™ DNA Polymerase (Takara Bio, Inc.). .. The thermocycling conditions were as follows: 94°C for 5 min followed by 30 cycles of 94°C for 30 sec, 60°C for 30 sec, 72°C for 60 sec, and maintenance at 72°C for 10 min. PCR products were resolved by 2% agarose gel electrophoresis, and visualized by staining with ethidium bromide.

    Article Title: Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
    Article Snippet: Prohibitin cDNA was amplified by Z-Taq DNA polymerase (TaKaRa, Otsu, Japan) with prohibitin-specific sense (5′-CAGAGTGGAAGCAGGTGTGAG-3′) and antisense adaptor-containing (5′-GGCCACGCGTCCGACTAGTAC-3′) primers. .. The PCR product was detected using 1.0% agarose gel electrophoresis and purified using a GFXTM column (GE Healthcare Bioscience, Piscataway, NJ, U.S.A.).

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The PCR products were excised from an agarose gel and then purified using a Zymoclean kit (Zymo Research) as indicated by the manufacturer.

    Produced:

    Article Title: Physical Map Location of the Multicopy Genes Coding for Ammonia Monooxygenase and Hydroxylamine Oxidoreductase in the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain ENI-11
    Article Snippet: PFGE of the Xba I and Asc I digests of the ENI-11 genomic DNA produced at least 31 and 22 fragments ranging from 430 to 10 kb, respectively. .. PCR was performed with respective sets of oligonucleotide primers and a Takara Z Taq DNA polymerase (Takara Shuzo Co., Shiga, Japan) on a DNA thermal cycler (Perkin-Elmer).

    Article Title: Rapid emergence of a virulent PB2 E627K variant during adaptation of highly pathogenic avian influenza H7N7 virus to mice
    Article Snippet: RNA isolation, RT-PCR amplification, and DNA sequencing Viral RNA was isolated from allantoic fluid of infected chicken eggs using a High Pure Viral RNA kit (Roche) according to the manufacturer’s protocol. cDNA was synthesized using Superscript II (Invitrogen) and amplified by PCR using Z -Taq polymerase (Takara Bio Inc.) and primers matching the noncoding sequence of each gene segment. .. The cDNA of maH7N7 was sequenced by direct dideoxy-terminated cycle sequencing using BigDye terminator v1.1 sequencing kit (Applied Biosystems) and a 3130 genetic analyzer sequencer (Applied Biosystems). cDNAs of chH7N7 virus were produced by PCR, isolated and cloned into the pGEM-T vector (Promega).

    Concentration Assay:

    Article Title: Comparison of Genome Sequences of Single-Stranded RNA Viruses Infecting the Bivalve-Killing Dinoflagellate Heterocapsa circularisquama
    Article Snippet: .. The second PCR amplification was performed using a 50-μl mixture containing 1.2 μl of diluted product from the first PCR, 1× Z- Taq buffer (Takara Bio Inc.), each deoxynucleoside triphosphate at a concentration of 200 μM, 4 pmol of primers MCPF2 and MCPR1, and 1.25 U of Z- Taq DNA polymerase (Takara Bio Inc.) and the GeneAmp PCR System 9700 (Applied Biosystems); it consisted of 35 cycles of denaturation at 98°C for 1 s, annealing at 58°C for 2 s, and extension at 72°C for 10 s. The resultant products were electrophoresed in 2% (wt/vol) agarose S gels (Nippon Gene Co., Ltd.). .. The nucleic acids were visualized by ethidium bromide staining.

    Staining:

    Article Title: Antagonistic effects of exogenous Slit2 on VEGF-induced choroidal endothelial cell migration and tube formation
    Article Snippet: The mRNA expression levels of Slit2, Robo1, Robo4 and β-actin were assessed via PCR amplification of target cDNA sequences using TaKaRa Z-Taq™ DNA Polymerase (Takara Bio, Inc.). .. The thermocycling conditions were as follows: 94°C for 5 min followed by 30 cycles of 94°C for 30 sec, 60°C for 30 sec, 72°C for 60 sec, and maintenance at 72°C for 10 min. PCR products were resolved by 2% agarose gel electrophoresis, and visualized by staining with ethidium bromide.

    Article Title: Comparison of Genome Sequences of Single-Stranded RNA Viruses Infecting the Bivalve-Killing Dinoflagellate Heterocapsa circularisquama
    Article Snippet: The second PCR amplification was performed using a 50-μl mixture containing 1.2 μl of diluted product from the first PCR, 1× Z- Taq buffer (Takara Bio Inc.), each deoxynucleoside triphosphate at a concentration of 200 μM, 4 pmol of primers MCPF2 and MCPR1, and 1.25 U of Z- Taq DNA polymerase (Takara Bio Inc.) and the GeneAmp PCR System 9700 (Applied Biosystems); it consisted of 35 cycles of denaturation at 98°C for 1 s, annealing at 58°C for 2 s, and extension at 72°C for 10 s. The resultant products were electrophoresed in 2% (wt/vol) agarose S gels (Nippon Gene Co., Ltd.). .. The nucleic acids were visualized by ethidium bromide staining.

    Variant Assay:

    Article Title: Antagonistic Regulation of Cell-Matrix Adhesion by FosB and ?FosB/?2?FosB Encoded by Alternatively Spliced Forms of fosB Transcripts
    Article Snippet: The second PCR was performed with primer FB10 and with the resulting PCR product as a Mega-primer and using the same template as with the first PCR using zTaq DNA polymerase (Takara Bio). .. The d2EGFP cDNA used was excised from the d2EGFP-N1 vector, which encodes a destabilized variant of EGFP (Clontech, Palo Alto, CA).

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    TaKaRa dna polymerase
    The Y. pestis <t>caf1A</t> ::IS 1541 variant, strain CAC1, can cause bubonic plague in guinea pigs without reverting to an F1 + phenotype. A: Genomic <t>DNA</t> isolated from Y. pestis CO92 (wild type), Y. pestis CAC1, or plague bacteria that were isolated from spleen,
    Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Y. pestis <t>caf1A</t> ::IS 1541 variant, strain CAC1, can cause bubonic plague in guinea pigs without reverting to an F1 + phenotype. A: Genomic <t>DNA</t> isolated from Y. pestis CO92 (wild type), Y. pestis CAC1, or plague bacteria that were isolated from spleen,
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    TaKaRa z taq
    The Y. pestis <t>caf1A</t> ::IS 1541 variant, strain CAC1, can cause bubonic plague in guinea pigs without reverting to an F1 + phenotype. A: Genomic <t>DNA</t> isolated from Y. pestis CO92 (wild type), Y. pestis CAC1, or plague bacteria that were isolated from spleen,
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    TaKaRa z taq polymerase
    The Y. pestis <t>caf1A</t> ::IS 1541 variant, strain CAC1, can cause bubonic plague in guinea pigs without reverting to an F1 + phenotype. A: Genomic <t>DNA</t> isolated from Y. pestis CO92 (wild type), Y. pestis CAC1, or plague bacteria that were isolated from spleen,
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    The Y. pestis caf1A ::IS 1541 variant, strain CAC1, can cause bubonic plague in guinea pigs without reverting to an F1 + phenotype. A: Genomic DNA isolated from Y. pestis CO92 (wild type), Y. pestis CAC1, or plague bacteria that were isolated from spleen,

    Journal: The American Journal of Pathology

    Article Title: Plague in Guinea Pigs and Its Prevention by Subunit Vaccines

    doi: 10.1016/j.ajpath.2010.12.028

    Figure Lengend Snippet: The Y. pestis caf1A ::IS 1541 variant, strain CAC1, can cause bubonic plague in guinea pigs without reverting to an F1 + phenotype. A: Genomic DNA isolated from Y. pestis CO92 (wild type), Y. pestis CAC1, or plague bacteria that were isolated from spleen,

    Article Snippet: Presence of the IS 1541 element in the caf1A gene was determined by PCR. caf1A was amplified using a DNA polymerase (Z-Taq; Takara Bio Inc., Otsu, Japan) and the following primers: 5′Caf1A-SphI (5′-TAGCATGCATGAGGTATTCAAAGCTGTTCC-3′) and 3′Caf1A-SacI (5′-TAGAGCTCTCAGTTATTTAAGATGCAGG-3′).

    Techniques: Variant Assay, Isolation