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z rouxii genomic dna  (ATCC)


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    ATCC z rouxii genomic dna
    eEF3 from the fungal species selected for this study.
    Z Rouxii Genomic Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z rouxii genomic dna/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z rouxii genomic dna - by Bioz Stars, 2024-12
    86/100 stars

    Images

    1) Product Images from "The gene YEF3 function encoding translation elongation factor eEF3 is partially conserved across fungi"

    Article Title: The gene YEF3 function encoding translation elongation factor eEF3 is partially conserved across fungi

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2024.1438900

    eEF3 from the fungal species selected for this study.
    Figure Legend Snippet: eEF3 from the fungal species selected for this study.

    Techniques Used:

    Strains used in this study.
    Figure Legend Snippet: Strains used in this study.

    Techniques Used:

    eEF3 from different Ascomycota species differentially complements S. cerevisiae YEF3 . (A) The scheme of plasmids pVT-U- YEF3 containing the S. cerevisiae YEF3 ( left ), p301 -eEF3-HA HA-tagged versions of eEF3 DNAs from different species used to perform the phenotypic rescues (right) , and pSEC-TRP containing the A. nidulans eEF3-HA . (B) Western blotting analysis using an anti-HA antibody (up) and Coomassie blue stain of total protein extracted from different species (down). (C) Phenotypic rescue using eEF3 from Ascomycota species. Experiment controls: Empty vector ( p301 , negative control), S. cerevisiae (positive control). eEF3 DNAs from C. glabrata, Z. rouxii , and A. nidulans were tested. The media composition is shown. Plates S glu + ALH (p301- eEF3-HA or pSEC-TRP- eEF3-HA expression is off) and S gal + ALH (p301- eEF3-HA or pSEC-TRP- eEF3-HA expression is on) are growth controls. Plates S gal + ALHU + 5-FOA have expelled pVT-U- YEF3 , and the growth relies only on p301- eEF3-HA or pSEC-TRP- eEF3-HA ( Phenotypic rescue ). (D) Growth curves of yeast cells expressing eEF3 from S. cerevisiae, C. glabrata , or Z. rouxii . CG, Candida glabrata; ZR, Z. rouxii; SC, Saccharomyces cerevisiae; A, Adenine; L, Leucine; H, Histidine; U, Uracil; 5-FOA , 5-fluoroorotic acid hydrate.
    Figure Legend Snippet: eEF3 from different Ascomycota species differentially complements S. cerevisiae YEF3 . (A) The scheme of plasmids pVT-U- YEF3 containing the S. cerevisiae YEF3 ( left ), p301 -eEF3-HA HA-tagged versions of eEF3 DNAs from different species used to perform the phenotypic rescues (right) , and pSEC-TRP containing the A. nidulans eEF3-HA . (B) Western blotting analysis using an anti-HA antibody (up) and Coomassie blue stain of total protein extracted from different species (down). (C) Phenotypic rescue using eEF3 from Ascomycota species. Experiment controls: Empty vector ( p301 , negative control), S. cerevisiae (positive control). eEF3 DNAs from C. glabrata, Z. rouxii , and A. nidulans were tested. The media composition is shown. Plates S glu + ALH (p301- eEF3-HA or pSEC-TRP- eEF3-HA expression is off) and S gal + ALH (p301- eEF3-HA or pSEC-TRP- eEF3-HA expression is on) are growth controls. Plates S gal + ALHU + 5-FOA have expelled pVT-U- YEF3 , and the growth relies only on p301- eEF3-HA or pSEC-TRP- eEF3-HA ( Phenotypic rescue ). (D) Growth curves of yeast cells expressing eEF3 from S. cerevisiae, C. glabrata , or Z. rouxii . CG, Candida glabrata; ZR, Z. rouxii; SC, Saccharomyces cerevisiae; A, Adenine; L, Leucine; H, Histidine; U, Uracil; 5-FOA , 5-fluoroorotic acid hydrate.

    Techniques Used: Western Blot, Staining, Plasmid Preparation, Negative Control, Positive Control, Expressing



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    Image Search Results


    eEF3 from the fungal species selected for this study.

    Journal: Frontiers in Microbiology

    Article Title: The gene YEF3 function encoding translation elongation factor eEF3 is partially conserved across fungi

    doi: 10.3389/fmicb.2024.1438900

    Figure Lengend Snippet: eEF3 from the fungal species selected for this study.

    Article Snippet: Z. rouxii genomic DNA was purchased from the American Type Culture Collection (ATCC).

    Techniques:

    Strains used in this study.

    Journal: Frontiers in Microbiology

    Article Title: The gene YEF3 function encoding translation elongation factor eEF3 is partially conserved across fungi

    doi: 10.3389/fmicb.2024.1438900

    Figure Lengend Snippet: Strains used in this study.

    Article Snippet: Z. rouxii genomic DNA was purchased from the American Type Culture Collection (ATCC).

    Techniques:

    eEF3 from different Ascomycota species differentially complements S. cerevisiae YEF3 . (A) The scheme of plasmids pVT-U- YEF3 containing the S. cerevisiae YEF3 ( left ), p301 -eEF3-HA HA-tagged versions of eEF3 DNAs from different species used to perform the phenotypic rescues (right) , and pSEC-TRP containing the A. nidulans eEF3-HA . (B) Western blotting analysis using an anti-HA antibody (up) and Coomassie blue stain of total protein extracted from different species (down). (C) Phenotypic rescue using eEF3 from Ascomycota species. Experiment controls: Empty vector ( p301 , negative control), S. cerevisiae (positive control). eEF3 DNAs from C. glabrata, Z. rouxii , and A. nidulans were tested. The media composition is shown. Plates S glu + ALH (p301- eEF3-HA or pSEC-TRP- eEF3-HA expression is off) and S gal + ALH (p301- eEF3-HA or pSEC-TRP- eEF3-HA expression is on) are growth controls. Plates S gal + ALHU + 5-FOA have expelled pVT-U- YEF3 , and the growth relies only on p301- eEF3-HA or pSEC-TRP- eEF3-HA ( Phenotypic rescue ). (D) Growth curves of yeast cells expressing eEF3 from S. cerevisiae, C. glabrata , or Z. rouxii . CG, Candida glabrata; ZR, Z. rouxii; SC, Saccharomyces cerevisiae; A, Adenine; L, Leucine; H, Histidine; U, Uracil; 5-FOA , 5-fluoroorotic acid hydrate.

    Journal: Frontiers in Microbiology

    Article Title: The gene YEF3 function encoding translation elongation factor eEF3 is partially conserved across fungi

    doi: 10.3389/fmicb.2024.1438900

    Figure Lengend Snippet: eEF3 from different Ascomycota species differentially complements S. cerevisiae YEF3 . (A) The scheme of plasmids pVT-U- YEF3 containing the S. cerevisiae YEF3 ( left ), p301 -eEF3-HA HA-tagged versions of eEF3 DNAs from different species used to perform the phenotypic rescues (right) , and pSEC-TRP containing the A. nidulans eEF3-HA . (B) Western blotting analysis using an anti-HA antibody (up) and Coomassie blue stain of total protein extracted from different species (down). (C) Phenotypic rescue using eEF3 from Ascomycota species. Experiment controls: Empty vector ( p301 , negative control), S. cerevisiae (positive control). eEF3 DNAs from C. glabrata, Z. rouxii , and A. nidulans were tested. The media composition is shown. Plates S glu + ALH (p301- eEF3-HA or pSEC-TRP- eEF3-HA expression is off) and S gal + ALH (p301- eEF3-HA or pSEC-TRP- eEF3-HA expression is on) are growth controls. Plates S gal + ALHU + 5-FOA have expelled pVT-U- YEF3 , and the growth relies only on p301- eEF3-HA or pSEC-TRP- eEF3-HA ( Phenotypic rescue ). (D) Growth curves of yeast cells expressing eEF3 from S. cerevisiae, C. glabrata , or Z. rouxii . CG, Candida glabrata; ZR, Z. rouxii; SC, Saccharomyces cerevisiae; A, Adenine; L, Leucine; H, Histidine; U, Uracil; 5-FOA , 5-fluoroorotic acid hydrate.

    Article Snippet: Z. rouxii genomic DNA was purchased from the American Type Culture Collection (ATCC).

    Techniques: Western Blot, Staining, Plasmid Preparation, Negative Control, Positive Control, Expressing

    QOR gene expression of positive clones and native Zygosaccharomyces rouxii , based on the qRT–PCR results. *Representing p < .05, **representing p < .01, and ***representing p < .001 are considered as statistically significant, respectively, the same as below. QOR, quinone oxidoreductase.

    Journal: Food Science & Nutrition

    Article Title: Construction of a Zygosaccharomyces rouxii strain overexpressing the QOR gene for increased HDMF production

    doi: 10.1002/fsn3.4109

    Figure Lengend Snippet: QOR gene expression of positive clones and native Zygosaccharomyces rouxii , based on the qRT–PCR results. *Representing p < .05, **representing p < .01, and ***representing p < .001 are considered as statistically significant, respectively, the same as below. QOR, quinone oxidoreductase.

    Article Snippet: The QOR gene sequence (AC: XM_002494472.1) from native Z. rouxii was first obtained from the NCBI database, and primers were designed using Primer Premier 5.0 Software (Premier Biosoft Co. Ltd., Palo Alto, California, USA) (Table ).

    Techniques: Expressing, Clone Assay, Quantitative RT-PCR