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Journal: Nature Communications
Article Title: Nano-kinetic implant surface mediates osteo-antibacterial synergy through electromagnetic-mechanical coupling
doi: 10.1038/s41467-026-78462-4
Figure Lengend Snippet: A Representative western blot images of Piezo1 in BMSCs. B Quantification of Piezo1 protein levels; n = 3 biological replications, data were presented as mean values ± SD; error bars = SD. C Schematic illustrating Piezo1-mediated mechanotransduction. Created in BioRender. Chen, Y. (2026) https://BioRender.com/nwwp37d . D Real-time intracellular calcium dynamics in BMSCs treated with 0, 5, 10, or 20 μM Yoda1. E Calcium responses in BMSCs pre-treated with Dooku1 (0-20 μM) followed by 10 μM Yoda1. F Alizarin Red S and G ALP staining of BMSCs treated with Yoda1 and/or Dooku1. H Calcium dynamics in BMSCs cultured on Ti, UMTS, or MMTS with/without EMF. I Calcium responses in Dooku1-pre-treated BMSCs under the same conditions. J Fluorescent staining of primary cilia (green, anti-acetylated tubulin, indicated with white arrows), and nuclei (blue, DAPI). K Alizarin red staining and L ALP staining. M Schematic illustrating the importance of primary cilia in BMSCs in perceiving mechanical stimulation. a.u. denotes arbitrary units. Created in BioRender. Chen, Y. (2026) https://BioRender.com/tb5ct1c . Data were compared by one-way ANOVA followed by Tukey’s multiple comparison test. Source data are provided as a file.
Article Snippet:
Techniques: Western Blot, Staining, Cell Culture, Comparison
Journal: Nature Communications
Article Title: Nano-kinetic implant surface mediates osteo-antibacterial synergy through electromagnetic-mechanical coupling
doi: 10.1038/s41467-026-78462-4
Figure Lengend Snippet: A Representative western blot images of Piezo1 in BMSCs. B Quantification of Piezo1 protein levels; n = 3 biological replications, data were presented as mean values ± SD; error bars = SD. C Schematic illustrating Piezo1-mediated mechanotransduction. Created in BioRender. Chen, Y. (2026) https://BioRender.com/nwwp37d . D Real-time intracellular calcium dynamics in BMSCs treated with 0, 5, 10, or 20 μM Yoda1. E Calcium responses in BMSCs pre-treated with Dooku1 (0-20 μM) followed by 10 μM Yoda1. F Alizarin Red S and G ALP staining of BMSCs treated with Yoda1 and/or Dooku1. H Calcium dynamics in BMSCs cultured on Ti, UMTS, or MMTS with/without EMF. I Calcium responses in Dooku1-pre-treated BMSCs under the same conditions. J Fluorescent staining of primary cilia (green, anti-acetylated tubulin, indicated with white arrows), and nuclei (blue, DAPI). K Alizarin red staining and L ALP staining. M Schematic illustrating the importance of primary cilia in BMSCs in perceiving mechanical stimulation. a.u. denotes arbitrary units. Created in BioRender. Chen, Y. (2026) https://BioRender.com/tb5ct1c . Data were compared by one-way ANOVA followed by Tukey’s multiple comparison test. Source data are provided as a file.
Article Snippet: Piezo1 activator Yoda1 (Cat No. HY-18723, MCE, China) and
Techniques: Western Blot, Staining, Cell Culture, Comparison