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TaKaRa y4rna r4
Quantitative RT-PCR. (A) Primer pairs. The sequences of the primers SL-forward_Y4_3 and <t>Y4RNA_R4</t> for amplification of the 31/32-nt Y4-RNA fragment and the sequences of the primers SL-forward_Y4_3 and Y4RNA_R5 for amplification of the full-length Y4-RNA are presented together with the sequence of Y4-RNA, a middle part of which is omitted. Three nucleotides are added to the 5′ terminus of each primer to stabilize the primer/template hybrid. (B) A representative RT-PCR data for the synthetic 31-nt Y4-RNA fragment (0.001, 0.01, 0.05, and 0.1 fmol) and saliva RNA (1 ng) from the examinees E1, E3, and E4. The right and left halves represent the data for the full-length Y4-RNA and the 31/32-nt fragment, respectively. (C) A standard curve was drawn using the RT-PCR data for the synthetic 31-nt Y4-RNA fragment. (D) Quantitation of the 31/32-nt Y4-RNA fragment in saliva RNA from the examinees E1, E3, and E4. Error bars denote standard deviations (n = 3).
Y4rna R4, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: The Y4-RNA fragment, a potential diagnostic marker, exists in saliva

Journal: Non-coding RNA Research

doi: 10.1016/j.ncrna.2017.07.002

Quantitative RT-PCR. (A) Primer pairs. The sequences of the primers SL-forward_Y4_3 and Y4RNA_R4 for amplification of the 31/32-nt Y4-RNA fragment and the sequences of the primers SL-forward_Y4_3 and Y4RNA_R5 for amplification of the full-length Y4-RNA are presented together with the sequence of Y4-RNA, a middle part of which is omitted. Three nucleotides are added to the 5′ terminus of each primer to stabilize the primer/template hybrid. (B) A representative RT-PCR data for the synthetic 31-nt Y4-RNA fragment (0.001, 0.01, 0.05, and 0.1 fmol) and saliva RNA (1 ng) from the examinees E1, E3, and E4. The right and left halves represent the data for the full-length Y4-RNA and the 31/32-nt fragment, respectively. (C) A standard curve was drawn using the RT-PCR data for the synthetic 31-nt Y4-RNA fragment. (D) Quantitation of the 31/32-nt Y4-RNA fragment in saliva RNA from the examinees E1, E3, and E4. Error bars denote standard deviations (n = 3).
Figure Legend Snippet: Quantitative RT-PCR. (A) Primer pairs. The sequences of the primers SL-forward_Y4_3 and Y4RNA_R4 for amplification of the 31/32-nt Y4-RNA fragment and the sequences of the primers SL-forward_Y4_3 and Y4RNA_R5 for amplification of the full-length Y4-RNA are presented together with the sequence of Y4-RNA, a middle part of which is omitted. Three nucleotides are added to the 5′ terminus of each primer to stabilize the primer/template hybrid. (B) A representative RT-PCR data for the synthetic 31-nt Y4-RNA fragment (0.001, 0.01, 0.05, and 0.1 fmol) and saliva RNA (1 ng) from the examinees E1, E3, and E4. The right and left halves represent the data for the full-length Y4-RNA and the 31/32-nt fragment, respectively. (C) A standard curve was drawn using the RT-PCR data for the synthetic 31-nt Y4-RNA fragment. (D) Quantitation of the 31/32-nt Y4-RNA fragment in saliva RNA from the examinees E1, E3, and E4. Error bars denote standard deviations (n = 3).

Techniques Used: Quantitative RT-PCR, Amplification, Sequencing, Reverse Transcription Polymerase Chain Reaction, Quantitation Assay

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Reverse Transcription Polymerase Chain Reaction:

Article Title: The Y4-RNA fragment, a potential diagnostic marker, exists in saliva
Article Snippet: .. 2.4 Reverse transcription (RT) PCR An RNA test sample was reverse transcribed using a reverse primer, Y4RNA_R4 or Y4RNA_R5, with a PrimeScriptTM RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio, Otsu, Japan) according to manufacturer's protocol. .. Subsequently, a real time PCR for the obtained cDNA was conducted using the primer pair SL-forward_Y4_3/Y4RNA_R4 or SL-forward_Y4_3/Y4RNA_R5 and SYBR Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio, Otsu, Japan) with a Thermal Cycler Dice Real Time System (Takara Bio, Otsu, Japan) under the standard conditions according to manufacturer's protocol.

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    TaKaRa y4rna r4
    Quantitative RT-PCR. (A) Primer pairs. The sequences of the primers SL-forward_Y4_3 and <t>Y4RNA_R4</t> for amplification of the 31/32-nt Y4-RNA fragment and the sequences of the primers SL-forward_Y4_3 and Y4RNA_R5 for amplification of the full-length Y4-RNA are presented together with the sequence of Y4-RNA, a middle part of which is omitted. Three nucleotides are added to the 5′ terminus of each primer to stabilize the primer/template hybrid. (B) A representative RT-PCR data for the synthetic 31-nt Y4-RNA fragment (0.001, 0.01, 0.05, and 0.1 fmol) and saliva RNA (1 ng) from the examinees E1, E3, and E4. The right and left halves represent the data for the full-length Y4-RNA and the 31/32-nt fragment, respectively. (C) A standard curve was drawn using the RT-PCR data for the synthetic 31-nt Y4-RNA fragment. (D) Quantitation of the 31/32-nt Y4-RNA fragment in saliva RNA from the examinees E1, E3, and E4. Error bars denote standard deviations (n = 3).
    Y4rna R4, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/y4rna r4/product/TaKaRa
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    y4rna r4 - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    91
    TaKaRa primer pair sl forward y4 3 y4rna r4
    Quantitative RT-PCR. (A) Primer pairs. The sequences of the primers <t>SL-forward_Y4_3</t> and <t>Y4RNA_R4</t> for amplification of the 31/32-nt Y4-RNA fragment and the sequences of the primers SL-forward_Y4_3 and Y4RNA_R5 for amplification of the full-length Y4-RNA are presented together with the sequence of Y4-RNA, a middle part of which is omitted. Three nucleotides are added to the 5′ terminus of each primer to stabilize the primer/template hybrid. (B) A representative RT-PCR data for the synthetic 31-nt Y4-RNA fragment (0.001, 0.01, 0.05, and 0.1 fmol) and saliva RNA (1 ng) from the examinees E1, E3, and E4. The right and left halves represent the data for the full-length Y4-RNA and the 31/32-nt fragment, respectively. (C) A standard curve was drawn using the RT-PCR data for the synthetic 31-nt Y4-RNA fragment. (D) Quantitation of the 31/32-nt Y4-RNA fragment in saliva RNA from the examinees E1, E3, and E4. Error bars denote standard deviations (n = 3).
    Primer Pair Sl Forward Y4 3 Y4rna R4, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer pair sl forward y4 3 y4rna r4/product/TaKaRa
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primer pair sl forward y4 3 y4rna r4 - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

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    Quantitative RT-PCR. (A) Primer pairs. The sequences of the primers SL-forward_Y4_3 and Y4RNA_R4 for amplification of the 31/32-nt Y4-RNA fragment and the sequences of the primers SL-forward_Y4_3 and Y4RNA_R5 for amplification of the full-length Y4-RNA are presented together with the sequence of Y4-RNA, a middle part of which is omitted. Three nucleotides are added to the 5′ terminus of each primer to stabilize the primer/template hybrid. (B) A representative RT-PCR data for the synthetic 31-nt Y4-RNA fragment (0.001, 0.01, 0.05, and 0.1 fmol) and saliva RNA (1 ng) from the examinees E1, E3, and E4. The right and left halves represent the data for the full-length Y4-RNA and the 31/32-nt fragment, respectively. (C) A standard curve was drawn using the RT-PCR data for the synthetic 31-nt Y4-RNA fragment. (D) Quantitation of the 31/32-nt Y4-RNA fragment in saliva RNA from the examinees E1, E3, and E4. Error bars denote standard deviations (n = 3).

    Journal: Non-coding RNA Research

    Article Title: The Y4-RNA fragment, a potential diagnostic marker, exists in saliva

    doi: 10.1016/j.ncrna.2017.07.002

    Figure Lengend Snippet: Quantitative RT-PCR. (A) Primer pairs. The sequences of the primers SL-forward_Y4_3 and Y4RNA_R4 for amplification of the 31/32-nt Y4-RNA fragment and the sequences of the primers SL-forward_Y4_3 and Y4RNA_R5 for amplification of the full-length Y4-RNA are presented together with the sequence of Y4-RNA, a middle part of which is omitted. Three nucleotides are added to the 5′ terminus of each primer to stabilize the primer/template hybrid. (B) A representative RT-PCR data for the synthetic 31-nt Y4-RNA fragment (0.001, 0.01, 0.05, and 0.1 fmol) and saliva RNA (1 ng) from the examinees E1, E3, and E4. The right and left halves represent the data for the full-length Y4-RNA and the 31/32-nt fragment, respectively. (C) A standard curve was drawn using the RT-PCR data for the synthetic 31-nt Y4-RNA fragment. (D) Quantitation of the 31/32-nt Y4-RNA fragment in saliva RNA from the examinees E1, E3, and E4. Error bars denote standard deviations (n = 3).

    Article Snippet: 2.4 Reverse transcription (RT) PCR An RNA test sample was reverse transcribed using a reverse primer, Y4RNA_R4 or Y4RNA_R5, with a PrimeScriptTM RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio, Otsu, Japan) according to manufacturer's protocol.

    Techniques: Quantitative RT-PCR, Amplification, Sequencing, Reverse Transcription Polymerase Chain Reaction, Quantitation Assay

    Quantitative RT-PCR. (A) Primer pairs. The sequences of the primers SL-forward_Y4_3 and Y4RNA_R4 for amplification of the 31/32-nt Y4-RNA fragment and the sequences of the primers SL-forward_Y4_3 and Y4RNA_R5 for amplification of the full-length Y4-RNA are presented together with the sequence of Y4-RNA, a middle part of which is omitted. Three nucleotides are added to the 5′ terminus of each primer to stabilize the primer/template hybrid. (B) A representative RT-PCR data for the synthetic 31-nt Y4-RNA fragment (0.001, 0.01, 0.05, and 0.1 fmol) and saliva RNA (1 ng) from the examinees E1, E3, and E4. The right and left halves represent the data for the full-length Y4-RNA and the 31/32-nt fragment, respectively. (C) A standard curve was drawn using the RT-PCR data for the synthetic 31-nt Y4-RNA fragment. (D) Quantitation of the 31/32-nt Y4-RNA fragment in saliva RNA from the examinees E1, E3, and E4. Error bars denote standard deviations (n = 3).

    Journal: Non-coding RNA Research

    Article Title: The Y4-RNA fragment, a potential diagnostic marker, exists in saliva

    doi: 10.1016/j.ncrna.2017.07.002

    Figure Lengend Snippet: Quantitative RT-PCR. (A) Primer pairs. The sequences of the primers SL-forward_Y4_3 and Y4RNA_R4 for amplification of the 31/32-nt Y4-RNA fragment and the sequences of the primers SL-forward_Y4_3 and Y4RNA_R5 for amplification of the full-length Y4-RNA are presented together with the sequence of Y4-RNA, a middle part of which is omitted. Three nucleotides are added to the 5′ terminus of each primer to stabilize the primer/template hybrid. (B) A representative RT-PCR data for the synthetic 31-nt Y4-RNA fragment (0.001, 0.01, 0.05, and 0.1 fmol) and saliva RNA (1 ng) from the examinees E1, E3, and E4. The right and left halves represent the data for the full-length Y4-RNA and the 31/32-nt fragment, respectively. (C) A standard curve was drawn using the RT-PCR data for the synthetic 31-nt Y4-RNA fragment. (D) Quantitation of the 31/32-nt Y4-RNA fragment in saliva RNA from the examinees E1, E3, and E4. Error bars denote standard deviations (n = 3).

    Article Snippet: Subsequently, a real time PCR for the obtained cDNA was conducted using the primer pair SL-forward_Y4_3/Y4RNA_R4 or SL-forward_Y4_3/Y4RNA_R5 and SYBR Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio, Otsu, Japan) with a Thermal Cycler Dice Real Time System (Takara Bio, Otsu, Japan) under the standard conditions according to manufacturer's protocol.

    Techniques: Quantitative RT-PCR, Amplification, Sequencing, Reverse Transcription Polymerase Chain Reaction, Quantitation Assay