pi5p polypiposome (Echelon Biosciences)


Structured Review

Pi5p Polypiposome, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pi5p polypiposome/product/Echelon Biosciences
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers"
Article Title: A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers
Journal: Scientific Reports
doi: 10.1038/s41598-017-16508-w

Figure Legend Snippet: MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) PolyPIPosome binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .
Techniques Used: Stripping Membranes, Binding Assay

Figure Legend Snippet: MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure .
Techniques Used: SDS Page

Figure Legend Snippet: γ-core motif sequences of MtDef5 are critical for membrane permeabilization, oligomerization and antifungal activity of MtDef5, but not for PIP binding. ( A ) Sequence of MtDef5 and its variants. Amino acid residues of the two γ-core motifs (highlighted) were mutated to alanine ( A ). ( B ) Quantitative assessment of the in vitro antifungal activity of MtDef5 and its variants against F . graminearum conidia after 20 h of treatment. Values are means of three replications. Error bars indicate standard deviations. ( C ) Quantitative measurement of fluorescence emitted by hyphae treated with 0.75 µM MtDef5 or its variants plus 0.5 µM of SYTOX Green. Values are means of three replications. Error bars indicate standard deviations. ( D ) Interaction of MtDef5 and it variants to phospholipids. PIP strip shows that MtDef5 and it variants strongly bind to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( E ) PolyPIPosome binding assays of MtDef5 and its γ-core motif variants. It shows that MtDef5 binds to PI3P and PI4P with higher affinity than PI5P. It also binds to PI. The full length gels are presented in Supplementary Figure . ( F ) Oligomerization of MtDef5 γ-core motif variants in presence of PI(3)P. MtDef5_V1 variant containing the H36A, R37A, H93A and R94A substitutions loses its ability to oligomerize in presence of PI3P. The full length gel is presented in Supplementary Figure .
Techniques Used: Activity Assay, Binding Assay, Sequencing, In Vitro, Fluorescence, Stripping Membranes, Variant Assay
pi5p polypiposome (Echelon Biosciences)


Structured Review

Pi5p Polypiposome, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pi5p polypiposome/product/Echelon Biosciences
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers"
Article Title: A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers
Journal: Scientific Reports
doi: 10.1038/s41598-017-16508-w

Figure Legend Snippet: MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) PolyPIPosome binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .
Techniques Used: Stripping Membranes, Binding Assay

Figure Legend Snippet: MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure .
Techniques Used: SDS Page

Figure Legend Snippet: γ-core motif sequences of MtDef5 are critical for membrane permeabilization, oligomerization and antifungal activity of MtDef5, but not for PIP binding. ( A ) Sequence of MtDef5 and its variants. Amino acid residues of the two γ-core motifs (highlighted) were mutated to alanine ( A ). ( B ) Quantitative assessment of the in vitro antifungal activity of MtDef5 and its variants against F . graminearum conidia after 20 h of treatment. Values are means of three replications. Error bars indicate standard deviations. ( C ) Quantitative measurement of fluorescence emitted by hyphae treated with 0.75 µM MtDef5 or its variants plus 0.5 µM of SYTOX Green. Values are means of three replications. Error bars indicate standard deviations. ( D ) Interaction of MtDef5 and it variants to phospholipids. PIP strip shows that MtDef5 and it variants strongly bind to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( E ) PolyPIPosome binding assays of MtDef5 and its γ-core motif variants. It shows that MtDef5 binds to PI3P and PI4P with higher affinity than PI5P. It also binds to PI. The full length gels are presented in Supplementary Figure . ( F ) Oligomerization of MtDef5 γ-core motif variants in presence of PI(3)P. MtDef5_V1 variant containing the H36A, R37A, H93A and R94A substitutions loses its ability to oligomerize in presence of PI3P. The full length gel is presented in Supplementary Figure .
Techniques Used: Activity Assay, Binding Assay, Sequencing, In Vitro, Fluorescence, Stripping Membranes, Variant Assay
polypiposomes (Echelon Biosciences)


Structured Review
Polypiposomes, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polypiposomes/product/Echelon Biosciences
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
polypiposomes (Echelon Biosciences)


Structured Review
Polypiposomes, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polypiposomes/product/Echelon Biosciences
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99