pi5p polypiposome  (Echelon Biosciences)


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    Structured Review

    Echelon Biosciences pi5p polypiposome
    MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and <t>PI5P</t> and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) <t>PolyPIPosome</t> binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .
    Pi5p Polypiposome, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi5p polypiposome/product/Echelon Biosciences
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pi5p polypiposome - by Bioz Stars, 2023-02
    88/100 stars

    Images

    1) Product Images from "A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers"

    Article Title: A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-16508-w

    MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) PolyPIPosome binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .
    Figure Legend Snippet: MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) PolyPIPosome binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .

    Techniques Used: Stripping Membranes, Binding Assay

    MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure .
    Figure Legend Snippet: MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure .

    Techniques Used: SDS Page

    γ-core motif sequences of MtDef5 are critical for membrane permeabilization, oligomerization and antifungal activity of MtDef5, but not for PIP binding. ( A ) Sequence of MtDef5 and its variants. Amino acid residues of the two γ-core motifs (highlighted) were mutated to alanine ( A ). ( B ) Quantitative assessment of the in vitro antifungal activity of MtDef5 and its variants against F . graminearum conidia after 20 h of treatment. Values are means of three replications. Error bars indicate standard deviations. ( C ) Quantitative measurement of fluorescence emitted by hyphae treated with 0.75 µM MtDef5 or its variants plus 0.5 µM of SYTOX Green. Values are means of three replications. Error bars indicate standard deviations. ( D ) Interaction of MtDef5 and it variants to phospholipids. PIP strip shows that MtDef5 and it variants strongly bind to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( E ) PolyPIPosome binding assays of MtDef5 and its γ-core motif variants. It shows that MtDef5 binds to PI3P and PI4P with higher affinity than PI5P. It also binds to PI. The full length gels are presented in Supplementary Figure . ( F ) Oligomerization of MtDef5 γ-core motif variants in presence of PI(3)P. MtDef5_V1 variant containing the H36A, R37A, H93A and R94A substitutions loses its ability to oligomerize in presence of PI3P. The full length gel is presented in Supplementary Figure .
    Figure Legend Snippet: γ-core motif sequences of MtDef5 are critical for membrane permeabilization, oligomerization and antifungal activity of MtDef5, but not for PIP binding. ( A ) Sequence of MtDef5 and its variants. Amino acid residues of the two γ-core motifs (highlighted) were mutated to alanine ( A ). ( B ) Quantitative assessment of the in vitro antifungal activity of MtDef5 and its variants against F . graminearum conidia after 20 h of treatment. Values are means of three replications. Error bars indicate standard deviations. ( C ) Quantitative measurement of fluorescence emitted by hyphae treated with 0.75 µM MtDef5 or its variants plus 0.5 µM of SYTOX Green. Values are means of three replications. Error bars indicate standard deviations. ( D ) Interaction of MtDef5 and it variants to phospholipids. PIP strip shows that MtDef5 and it variants strongly bind to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( E ) PolyPIPosome binding assays of MtDef5 and its γ-core motif variants. It shows that MtDef5 binds to PI3P and PI4P with higher affinity than PI5P. It also binds to PI. The full length gels are presented in Supplementary Figure . ( F ) Oligomerization of MtDef5 γ-core motif variants in presence of PI(3)P. MtDef5_V1 variant containing the H36A, R37A, H93A and R94A substitutions loses its ability to oligomerize in presence of PI3P. The full length gel is presented in Supplementary Figure .

    Techniques Used: Activity Assay, Binding Assay, Sequencing, In Vitro, Fluorescence, Stripping Membranes, Variant Assay

    pi5p polypiposome  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
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    Structured Review

    Echelon Biosciences pi5p polypiposome
    MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and <t>PI5P</t> and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) <t>PolyPIPosome</t> binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .
    Pi5p Polypiposome, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi5p polypiposome/product/Echelon Biosciences
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pi5p polypiposome - by Bioz Stars, 2023-02
    88/100 stars

    Images

    1) Product Images from "A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers"

    Article Title: A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-16508-w

    MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) PolyPIPosome binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .
    Figure Legend Snippet: MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) PolyPIPosome binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .

    Techniques Used: Stripping Membranes, Binding Assay

    MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure .
    Figure Legend Snippet: MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure .

    Techniques Used: SDS Page

    γ-core motif sequences of MtDef5 are critical for membrane permeabilization, oligomerization and antifungal activity of MtDef5, but not for PIP binding. ( A ) Sequence of MtDef5 and its variants. Amino acid residues of the two γ-core motifs (highlighted) were mutated to alanine ( A ). ( B ) Quantitative assessment of the in vitro antifungal activity of MtDef5 and its variants against F . graminearum conidia after 20 h of treatment. Values are means of three replications. Error bars indicate standard deviations. ( C ) Quantitative measurement of fluorescence emitted by hyphae treated with 0.75 µM MtDef5 or its variants plus 0.5 µM of SYTOX Green. Values are means of three replications. Error bars indicate standard deviations. ( D ) Interaction of MtDef5 and it variants to phospholipids. PIP strip shows that MtDef5 and it variants strongly bind to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( E ) PolyPIPosome binding assays of MtDef5 and its γ-core motif variants. It shows that MtDef5 binds to PI3P and PI4P with higher affinity than PI5P. It also binds to PI. The full length gels are presented in Supplementary Figure . ( F ) Oligomerization of MtDef5 γ-core motif variants in presence of PI(3)P. MtDef5_V1 variant containing the H36A, R37A, H93A and R94A substitutions loses its ability to oligomerize in presence of PI3P. The full length gel is presented in Supplementary Figure .
    Figure Legend Snippet: γ-core motif sequences of MtDef5 are critical for membrane permeabilization, oligomerization and antifungal activity of MtDef5, but not for PIP binding. ( A ) Sequence of MtDef5 and its variants. Amino acid residues of the two γ-core motifs (highlighted) were mutated to alanine ( A ). ( B ) Quantitative assessment of the in vitro antifungal activity of MtDef5 and its variants against F . graminearum conidia after 20 h of treatment. Values are means of three replications. Error bars indicate standard deviations. ( C ) Quantitative measurement of fluorescence emitted by hyphae treated with 0.75 µM MtDef5 or its variants plus 0.5 µM of SYTOX Green. Values are means of three replications. Error bars indicate standard deviations. ( D ) Interaction of MtDef5 and it variants to phospholipids. PIP strip shows that MtDef5 and it variants strongly bind to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( E ) PolyPIPosome binding assays of MtDef5 and its γ-core motif variants. It shows that MtDef5 binds to PI3P and PI4P with higher affinity than PI5P. It also binds to PI. The full length gels are presented in Supplementary Figure . ( F ) Oligomerization of MtDef5 γ-core motif variants in presence of PI(3)P. MtDef5_V1 variant containing the H36A, R37A, H93A and R94A substitutions loses its ability to oligomerize in presence of PI3P. The full length gel is presented in Supplementary Figure .

    Techniques Used: Activity Assay, Binding Assay, Sequencing, In Vitro, Fluorescence, Stripping Membranes, Variant Assay

    polypiposomes  (Echelon Biosciences)


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    Echelon Biosciences polypiposomes
    Polypiposomes, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypiposomes/product/Echelon Biosciences
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polypiposomes - by Bioz Stars, 2023-02
    88/100 stars

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    polypiposomes  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
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    Structured Review

    Echelon Biosciences polypiposomes
    Polypiposomes, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypiposomes/product/Echelon Biosciences
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polypiposomes - by Bioz Stars, 2023-02
    88/100 stars

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    Echelon Biosciences pi5p polypiposome
    MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and <t>PI5P</t> and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) <t>PolyPIPosome</t> binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .
    Pi5p Polypiposome, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi5p polypiposome/product/Echelon Biosciences
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pi5p polypiposome - by Bioz Stars, 2023-02
    88/100 stars
      Buy from Supplier

    88
    Echelon Biosciences polypiposomes
    MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and <t>PI5P</t> and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) <t>PolyPIPosome</t> binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .
    Polypiposomes, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypiposomes/product/Echelon Biosciences
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polypiposomes - by Bioz Stars, 2023-02
    88/100 stars
      Buy from Supplier

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    MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) PolyPIPosome binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .

    Journal: Scientific Reports

    Article Title: A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers

    doi: 10.1038/s41598-017-16508-w

    Figure Lengend Snippet: MtDef5 binds to multiple phospholipids but with higher affinity for PIP. ( A ) PIP strip showing the binding of MtDef5 to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( B ) PIP array displays the relative binding of MtDef5 to phospholipids. ( C ) PolyPIPosome binding assay shows that MtDef5 binds with higher affinity to PI3P and PI4P as compared to PI5P. The full length gel is presented in Supplementary Figure .

    Article Snippet: PolyPIPosome binding assays were performed in 200 µL of binding buffer (20 mM HEPES pH 7.4, 120 mM NaCl, 1 mM EGTA, 1 mM MgCl 2 , 1 mg/mL BSA, 0.2 mM CaCl 2 , 5 mM KCl) containing 10 µL each of control PolyPIPosome, PI PolyPIPosome, PI3P PolyPIPosome, PI4P PolyPIPosomes and PI5P PolyPIPosome (Echelon Biosciences).

    Techniques: Stripping Membranes, Binding Assay

    MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure .

    Journal: Scientific Reports

    Article Title: A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers

    doi: 10.1038/s41598-017-16508-w

    Figure Lengend Snippet: MtDef5 forms oligomers only in presence of PIP. ( A ) MtDef5 forms higher order oligomers in presence of PI3P, PI4P and PI5P as revealed by a protein cross-linking with BS followed by SDS-PAGE and visualized using a Bio-Rad ChemiDoc XRS + system. ( B ) MtDef5 also forms oligomers in presence of PA and PI. The full length gel is presented in Supplementary Figure .

    Article Snippet: PolyPIPosome binding assays were performed in 200 µL of binding buffer (20 mM HEPES pH 7.4, 120 mM NaCl, 1 mM EGTA, 1 mM MgCl 2 , 1 mg/mL BSA, 0.2 mM CaCl 2 , 5 mM KCl) containing 10 µL each of control PolyPIPosome, PI PolyPIPosome, PI3P PolyPIPosome, PI4P PolyPIPosomes and PI5P PolyPIPosome (Echelon Biosciences).

    Techniques: SDS Page

    γ-core motif sequences of MtDef5 are critical for membrane permeabilization, oligomerization and antifungal activity of MtDef5, but not for PIP binding. ( A ) Sequence of MtDef5 and its variants. Amino acid residues of the two γ-core motifs (highlighted) were mutated to alanine ( A ). ( B ) Quantitative assessment of the in vitro antifungal activity of MtDef5 and its variants against F . graminearum conidia after 20 h of treatment. Values are means of three replications. Error bars indicate standard deviations. ( C ) Quantitative measurement of fluorescence emitted by hyphae treated with 0.75 µM MtDef5 or its variants plus 0.5 µM of SYTOX Green. Values are means of three replications. Error bars indicate standard deviations. ( D ) Interaction of MtDef5 and it variants to phospholipids. PIP strip shows that MtDef5 and it variants strongly bind to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( E ) PolyPIPosome binding assays of MtDef5 and its γ-core motif variants. It shows that MtDef5 binds to PI3P and PI4P with higher affinity than PI5P. It also binds to PI. The full length gels are presented in Supplementary Figure . ( F ) Oligomerization of MtDef5 γ-core motif variants in presence of PI(3)P. MtDef5_V1 variant containing the H36A, R37A, H93A and R94A substitutions loses its ability to oligomerize in presence of PI3P. The full length gel is presented in Supplementary Figure .

    Journal: Scientific Reports

    Article Title: A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers

    doi: 10.1038/s41598-017-16508-w

    Figure Lengend Snippet: γ-core motif sequences of MtDef5 are critical for membrane permeabilization, oligomerization and antifungal activity of MtDef5, but not for PIP binding. ( A ) Sequence of MtDef5 and its variants. Amino acid residues of the two γ-core motifs (highlighted) were mutated to alanine ( A ). ( B ) Quantitative assessment of the in vitro antifungal activity of MtDef5 and its variants against F . graminearum conidia after 20 h of treatment. Values are means of three replications. Error bars indicate standard deviations. ( C ) Quantitative measurement of fluorescence emitted by hyphae treated with 0.75 µM MtDef5 or its variants plus 0.5 µM of SYTOX Green. Values are means of three replications. Error bars indicate standard deviations. ( D ) Interaction of MtDef5 and it variants to phospholipids. PIP strip shows that MtDef5 and it variants strongly bind to PI3P, PI4P and PI5P and also to PI4,5P 2 , PI3,5P 2 , PA and PS. ( E ) PolyPIPosome binding assays of MtDef5 and its γ-core motif variants. It shows that MtDef5 binds to PI3P and PI4P with higher affinity than PI5P. It also binds to PI. The full length gels are presented in Supplementary Figure . ( F ) Oligomerization of MtDef5 γ-core motif variants in presence of PI(3)P. MtDef5_V1 variant containing the H36A, R37A, H93A and R94A substitutions loses its ability to oligomerize in presence of PI3P. The full length gel is presented in Supplementary Figure .

    Article Snippet: PolyPIPosome binding assays were performed in 200 µL of binding buffer (20 mM HEPES pH 7.4, 120 mM NaCl, 1 mM EGTA, 1 mM MgCl 2 , 1 mg/mL BSA, 0.2 mM CaCl 2 , 5 mM KCl) containing 10 µL each of control PolyPIPosome, PI PolyPIPosome, PI3P PolyPIPosome, PI4P PolyPIPosomes and PI5P PolyPIPosome (Echelon Biosciences).

    Techniques: Activity Assay, Binding Assay, Sequencing, In Vitro, Fluorescence, Stripping Membranes, Variant Assay