Structured Review

Bethyl xrn1
MARF1 and <t>XRN1</t> use analogous motifs to interact with EDC4. ( A ) Schematic diagram for MARF1 and XRN1 domain structures. ( B ) Phylogenetic alignment of the candidate C-terminal distal region that mediates MARF1-EDC4 interactions and the XRN1 EDC4-binding motif (EBM) in H. sapiens ( Hs ), X. tropicalis ( Xt ), and D. rerio ( Dr ). Residues in blue are conservative substitutions that are analogous in both proteins, residues in red are identical residues between all orthologs for each protein. The (*) represents the C-terminus of the protein and an analogous phenylalanine is indicated with a red arrow located within the SLiM; LAΦNF. ( C and D ) Co-immunoprecipitation experiments performed by incubating HeLa cell extracts expressing the indicated FLAG-tagged proteins and incubated with FLAG antibody. Subsequent western blot analysis was performed to identify the presence of endogenous EDC4. ( E ) Images generated through confocal microscopy of immunofluorescent staining of HeLa cells transfected with FLAG-MARF1 variants. The merged image represents the overlap of the α-FLAG (red) and α-EDC4 (green) signals and scale bars represent 20 μm. White arrows represent P-bodies as defined by foci with strong signal overlap between red and green staining. ( F ) RL-MAML1 activity detected in extracts of transfected HeLa cells expressing the annotated proteins and FL as a transfection normalization control. Histograms represent the mean RL activity detected from three biological replicates, normalized to FL activity and the RL/FL ratio of a catalytically inactive MARF1 (MARF1 ΔNYN ) set to 100. Error bars represent the SEM and statistical significance was calculated using a two-tailed T-test comparing RL activity in the (+) to (-) EDC4 overexpression samples (*** P
Xrn1, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xrn1/product/Bethyl
Average 93 stars, based on 40 article reviews
Price from $9.99 to $1999.99
xrn1 - by Bioz Stars, 2022-11
93/100 stars

Images

1) Product Images from "P-bodies directly regulate MARF1-mediated mRNA decay in human cells"

Article Title: P-bodies directly regulate MARF1-mediated mRNA decay in human cells

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkac557

MARF1 and XRN1 use analogous motifs to interact with EDC4. ( A ) Schematic diagram for MARF1 and XRN1 domain structures. ( B ) Phylogenetic alignment of the candidate C-terminal distal region that mediates MARF1-EDC4 interactions and the XRN1 EDC4-binding motif (EBM) in H. sapiens ( Hs ), X. tropicalis ( Xt ), and D. rerio ( Dr ). Residues in blue are conservative substitutions that are analogous in both proteins, residues in red are identical residues between all orthologs for each protein. The (*) represents the C-terminus of the protein and an analogous phenylalanine is indicated with a red arrow located within the SLiM; LAΦNF. ( C and D ) Co-immunoprecipitation experiments performed by incubating HeLa cell extracts expressing the indicated FLAG-tagged proteins and incubated with FLAG antibody. Subsequent western blot analysis was performed to identify the presence of endogenous EDC4. ( E ) Images generated through confocal microscopy of immunofluorescent staining of HeLa cells transfected with FLAG-MARF1 variants. The merged image represents the overlap of the α-FLAG (red) and α-EDC4 (green) signals and scale bars represent 20 μm. White arrows represent P-bodies as defined by foci with strong signal overlap between red and green staining. ( F ) RL-MAML1 activity detected in extracts of transfected HeLa cells expressing the annotated proteins and FL as a transfection normalization control. Histograms represent the mean RL activity detected from three biological replicates, normalized to FL activity and the RL/FL ratio of a catalytically inactive MARF1 (MARF1 ΔNYN ) set to 100. Error bars represent the SEM and statistical significance was calculated using a two-tailed T-test comparing RL activity in the (+) to (-) EDC4 overexpression samples (*** P
Figure Legend Snippet: MARF1 and XRN1 use analogous motifs to interact with EDC4. ( A ) Schematic diagram for MARF1 and XRN1 domain structures. ( B ) Phylogenetic alignment of the candidate C-terminal distal region that mediates MARF1-EDC4 interactions and the XRN1 EDC4-binding motif (EBM) in H. sapiens ( Hs ), X. tropicalis ( Xt ), and D. rerio ( Dr ). Residues in blue are conservative substitutions that are analogous in both proteins, residues in red are identical residues between all orthologs for each protein. The (*) represents the C-terminus of the protein and an analogous phenylalanine is indicated with a red arrow located within the SLiM; LAΦNF. ( C and D ) Co-immunoprecipitation experiments performed by incubating HeLa cell extracts expressing the indicated FLAG-tagged proteins and incubated with FLAG antibody. Subsequent western blot analysis was performed to identify the presence of endogenous EDC4. ( E ) Images generated through confocal microscopy of immunofluorescent staining of HeLa cells transfected with FLAG-MARF1 variants. The merged image represents the overlap of the α-FLAG (red) and α-EDC4 (green) signals and scale bars represent 20 μm. White arrows represent P-bodies as defined by foci with strong signal overlap between red and green staining. ( F ) RL-MAML1 activity detected in extracts of transfected HeLa cells expressing the annotated proteins and FL as a transfection normalization control. Histograms represent the mean RL activity detected from three biological replicates, normalized to FL activity and the RL/FL ratio of a catalytically inactive MARF1 (MARF1 ΔNYN ) set to 100. Error bars represent the SEM and statistical significance was calculated using a two-tailed T-test comparing RL activity in the (+) to (-) EDC4 overexpression samples (*** P

Techniques Used: Binding Assay, Immunoprecipitation, Expressing, Incubation, Western Blot, Generated, Confocal Microscopy, Staining, Transfection, Activity Assay, Two Tailed Test, Over Expression

MARF1 and XRN1 competitively interact with EDC4 in a mutually exclusive manner. ( A ) Schematic diagram representing the reporter assay in ( B ), where the MARF1 C-term or the XRN1 EBM compete with low levels of full-length MARF1 for interacting with endogenous EDC4. ( B ) RL-MAML1 activity detected in extracts of transfected HeLa cells expressing the annotated proteins and FL as a transfection normalization control. Histograms represent the mean RL activity detected from three biological replicates, normalized to FL activity and the RL/FL ratio of a catalytically inactive MARF1 (MARF1 ΔNYN ) set to 100. Error bars represent the SEM and statistical significance was calculated using a two-tailed T-test comparing RL activity to the (-) samples for MARF1 WT samples (** P
Figure Legend Snippet: MARF1 and XRN1 competitively interact with EDC4 in a mutually exclusive manner. ( A ) Schematic diagram representing the reporter assay in ( B ), where the MARF1 C-term or the XRN1 EBM compete with low levels of full-length MARF1 for interacting with endogenous EDC4. ( B ) RL-MAML1 activity detected in extracts of transfected HeLa cells expressing the annotated proteins and FL as a transfection normalization control. Histograms represent the mean RL activity detected from three biological replicates, normalized to FL activity and the RL/FL ratio of a catalytically inactive MARF1 (MARF1 ΔNYN ) set to 100. Error bars represent the SEM and statistical significance was calculated using a two-tailed T-test comparing RL activity to the (-) samples for MARF1 WT samples (** P

Techniques Used: Reporter Assay, Activity Assay, Transfection, Expressing, Two Tailed Test

2) Product Images from "A local translation program regulates centriole amplification in the airway epithelium"

Article Title: A local translation program regulates centriole amplification in the airway epithelium

Journal: bioRxiv

doi: 10.1101/2022.01.18.476821

Apical granules lack enzymes for mRNA degradation. a) Each panel represents a 3D image of confocal Z-stack images of a single MCC in culture. Apically localized granules are highlighted by dashed lines. TNRC6A stained with human Index serum (18033). DCP1A, DDX6 and XRN1 are concentrated in randomly-localized granules (P-bodies). However, DCP1A and DDX6 are undetectable in apically granules. XRN1 are present in small number of apically granules at low levels. b) Co-localization profile of apically-localized or randomly-localized granules (P-bodies) within the same MCC indicating the different concentration of these proteins in these two classes of granules. Percentage of granules with double labeling signal.
Figure Legend Snippet: Apical granules lack enzymes for mRNA degradation. a) Each panel represents a 3D image of confocal Z-stack images of a single MCC in culture. Apically localized granules are highlighted by dashed lines. TNRC6A stained with human Index serum (18033). DCP1A, DDX6 and XRN1 are concentrated in randomly-localized granules (P-bodies). However, DCP1A and DDX6 are undetectable in apically granules. XRN1 are present in small number of apically granules at low levels. b) Co-localization profile of apically-localized or randomly-localized granules (P-bodies) within the same MCC indicating the different concentration of these proteins in these two classes of granules. Percentage of granules with double labeling signal.

Techniques Used: Staining, Concentration Assay, Labeling

3) Product Images from "Positive epistasis between viral polymerase and the 3′ untranslated region of its genome reveals the epidemiologic fitness of dengue virus"

Article Title: Positive epistasis between viral polymerase and the 3′ untranslated region of its genome reveals the epidemiologic fitness of dengue virus

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.1919287117

PR2B sfRNA is packaged inside E protein-containing infectious particles and transmitted into new susceptible cells. ( A ) Quantification of sfRNA:gRNA ratios in supernatant of A549 cells at 24 hpi by qPCR. ( B ) Quantification of gRNA:sfRNA ratios in supernatant of A549 cells on knockdown of CD9, CD63, CD81, and CD151. NTC served as a reference point for assessment of significance. ( C – E ) Quantification of gRNA copy numbers ( C ), sfRNA copy numbers ( D ), and sfRNA:gRNA ratios ( E ) from 4G2 pull-down precipitate. LOD, limit of detection; ND, no detection. PR1 served as a reference point for assessment of significance. ( F ) Western blots of CD63 and 4G2 in pull-down precipitate. ( G – I ) Quantification of gRNA ( G ), sfRNA ( H ), and sfRNA:gRNA ratios ( I ) in A549 cells at 2 hpi, after XRN1 knockdown. ( J ) Western blots of phosphorylated IRF3, IRF3, and LAMP-1 at 6 hpi with MOIs of 1 and 10 of mock, PR1, and PR1NS53UTR and cotreatment of NITD008. ( K ) Level of infection with MOIs of 1 and 10 of mock, PR1, and PR1NS53UTR quantified by immunofluorescence assay. Data are presented as mean ± SD. * P
Figure Legend Snippet: PR2B sfRNA is packaged inside E protein-containing infectious particles and transmitted into new susceptible cells. ( A ) Quantification of sfRNA:gRNA ratios in supernatant of A549 cells at 24 hpi by qPCR. ( B ) Quantification of gRNA:sfRNA ratios in supernatant of A549 cells on knockdown of CD9, CD63, CD81, and CD151. NTC served as a reference point for assessment of significance. ( C – E ) Quantification of gRNA copy numbers ( C ), sfRNA copy numbers ( D ), and sfRNA:gRNA ratios ( E ) from 4G2 pull-down precipitate. LOD, limit of detection; ND, no detection. PR1 served as a reference point for assessment of significance. ( F ) Western blots of CD63 and 4G2 in pull-down precipitate. ( G – I ) Quantification of gRNA ( G ), sfRNA ( H ), and sfRNA:gRNA ratios ( I ) in A549 cells at 2 hpi, after XRN1 knockdown. ( J ) Western blots of phosphorylated IRF3, IRF3, and LAMP-1 at 6 hpi with MOIs of 1 and 10 of mock, PR1, and PR1NS53UTR and cotreatment of NITD008. ( K ) Level of infection with MOIs of 1 and 10 of mock, PR1, and PR1NS53UTR quantified by immunofluorescence assay. Data are presented as mean ± SD. * P

Techniques Used: Real-time Polymerase Chain Reaction, Western Blot, Infection, Immunofluorescence

4) Product Images from "Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition"

Article Title: Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkw1284

XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P
Figure Legend Snippet: XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P

Techniques Used: Transfection, Cell Culture, Expressing, Marker, In Vitro, Invasion Assay, Produced, Staining

5) Product Images from "Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition"

Article Title: Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkw1284

XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P
Figure Legend Snippet: XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P

Techniques Used: Transfection, Cell Culture, Expressing, Marker, In Vitro, Invasion Assay, Produced, Staining

6) Product Images from "Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition"

Article Title: Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkw1284

XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P
Figure Legend Snippet: XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P

Techniques Used: Transfection, Cell Culture, Expressing, Marker, In Vitro, Invasion Assay, Produced, Staining

7) Product Images from "Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition"

Article Title: Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkw1284

XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P
Figure Legend Snippet: XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P

Techniques Used: Transfection, Cell Culture, Expressing, Marker, In Vitro, Invasion Assay, Produced, Staining

8) Product Images from "Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition"

Article Title: Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkw1284

XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P
Figure Legend Snippet: XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P

Techniques Used: Transfection, Cell Culture, Expressing, Marker, In Vitro, Invasion Assay, Produced, Staining

9) Product Images from "Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition"

Article Title: Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkw1284

XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P
Figure Legend Snippet: XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P

Techniques Used: Transfection, Cell Culture, Expressing, Marker, In Vitro, Invasion Assay, Produced, Staining

10) Product Images from "Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition"

Article Title: Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkw1284

XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P
Figure Legend Snippet: XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P

Techniques Used: Transfection, Cell Culture, Expressing, Marker, In Vitro, Invasion Assay, Produced, Staining

11) Product Images from "Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition"

Article Title: Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkw1284

XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P
Figure Legend Snippet: XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P

Techniques Used: Transfection, Cell Culture, Expressing, Marker, In Vitro, Invasion Assay, Produced, Staining

12) Product Images from "Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition"

Article Title: Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkw1284

XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P
Figure Legend Snippet: XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P

Techniques Used: Transfection, Cell Culture, Expressing, Marker, In Vitro, Invasion Assay, Produced, Staining

13) Product Images from "Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition"

Article Title: Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkw1284

XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P
Figure Legend Snippet: XRN1 regulates ex-miRNA decay in recipient cells ( A ) Relative quantification analysis of ex-miRNA-223-3p in A549 cells. siXRN1-transfected A549 cells co-cultured with PMN overnight were harvested at the indicated periods of time post PMN removal (Time Washed, T.W.). Results are representative of three biological replicates. In the right panel, immunoblot analysis of XRN1 expression. b-ACTIN served as an equal loading control. ( B ) Immunoblot analysis of FOXO1 and EMT marker expression levels. β-ACTIN served as an equal loading control. ( C ) In vitro invasion assay of siXRN1-transfected A549 cells. A549 cells co-cultured with SPN of PMN, produced in serum-free medium, were seeded in the upper part of transwells. The number of cells attached to the bottom of a Matrigel-coated membrane after 16 h was quantified after crystal violet staining. Data represent the quantification of five biological replicates, ‘centre values’ as mean and error bars as s.d. * for P

Techniques Used: Transfection, Cell Culture, Expressing, Marker, In Vitro, Invasion Assay, Produced, Staining

14) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

15) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

16) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

17) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

18) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

19) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

20) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

21) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

22) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

23) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

24) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

25) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

26) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

27) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

28) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

29) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

30) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

31) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

32) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

33) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

34) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

35) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

36) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

37) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

38) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

39) Product Images from "Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication"

Article Title: Dissecting the Roles of the 5′ Exoribonucleases Xrn1 and Xrn2 in Restricting Hepatitis C Virus Replication

Journal: Journal of Virology

doi: 10.1128/JVI.03692-14

Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus
Figure Legend Snippet: Impact of Xrn1 or Xrn2 depletion on replication of JFH1 and HJ3-5 viruses. (A) Experimental scheme. Huh-7 or Huh-7.5 cells were transfected with Xrn1- or Xrn2-specific siRNAs (or control siCtrl) twice prior to infection with either JFH1 or HJ3-5 virus

Techniques Used: Transfection, Infection

Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or
Figure Legend Snippet: Replication of GLuc reporter virus RNAs in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. The reporter virus genome organization is shown at the top, with GLuc sequence inserted between p7 and NS2A. Huh-7.5 cells were transfected with Xrn1- or

Techniques Used: Sequencing, Transfection

Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior
Figure Legend Snippet: Impact of miR-122 supplementation on replication of JFH1 and HJ3-5 viruses in Xrn1- and Xrn2-depleted cells. (A) Experimental scheme. Huh-7.5 cells were transfected with siRNAs specific for Xrn1 or Xrn2, both Xrn1 and Xrn2, or siCtrl 72 and 48 h prior

Techniques Used: Transfection

Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without
Figure Legend Snippet: Xrn1 and Xrn2 expression and intracellular localization in uninfected and HCV-infected Huh-7.5 cells. (A) Immunoblots of Xrn1 and Xrn2 in whole-cell lysates and isolated nuclear (Nuc) and cytoplasmic (Cyto) fractions from Huh-7.5 cells with and without

Techniques Used: Expressing, Infection, Western Blot, Isolation

Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection
Figure Legend Snippet: Impacts of Xrn1 and Xrn2 depletion on decay of HCV RNA following antiviral shutdown of new RNA synthesis. (A) Experimental scheme. Huh-7.5 cells were transfected twice with siRNAs specific for Xrn1 or Xrn2 (or siCtrl) 48 and again 24 h prior to infection

Techniques Used: Transfection, Infection

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • xrn1  (Bethyl)
    88
    Bethyl xrn1
    MARF1 and <t>XRN1</t> use analogous motifs to interact with EDC4. ( A ) Schematic diagram for MARF1 and XRN1 domain structures. ( B ) Phylogenetic alignment of the candidate C-terminal distal region that mediates MARF1-EDC4 interactions and the XRN1 EDC4-binding motif (EBM) in H. sapiens ( Hs ), X. tropicalis ( Xt ), and D. rerio ( Dr ). Residues in blue are conservative substitutions that are analogous in both proteins, residues in red are identical residues between all orthologs for each protein. The (*) represents the C-terminus of the protein and an analogous phenylalanine is indicated with a red arrow located within the SLiM; LAΦNF. ( C and D ) Co-immunoprecipitation experiments performed by incubating HeLa cell extracts expressing the indicated FLAG-tagged proteins and incubated with FLAG antibody. Subsequent western blot analysis was performed to identify the presence of endogenous EDC4. ( E ) Images generated through confocal microscopy of immunofluorescent staining of HeLa cells transfected with FLAG-MARF1 variants. The merged image represents the overlap of the α-FLAG (red) and α-EDC4 (green) signals and scale bars represent 20 μm. White arrows represent P-bodies as defined by foci with strong signal overlap between red and green staining. ( F ) RL-MAML1 activity detected in extracts of transfected HeLa cells expressing the annotated proteins and FL as a transfection normalization control. Histograms represent the mean RL activity detected from three biological replicates, normalized to FL activity and the RL/FL ratio of a catalytically inactive MARF1 (MARF1 ΔNYN ) set to 100. Error bars represent the SEM and statistical significance was calculated using a two-tailed T-test comparing RL activity in the (+) to (-) EDC4 overexpression samples (*** P
    Xrn1, supplied by Bethyl, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xrn1/product/Bethyl
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xrn1 - by Bioz Stars, 2022-11
    88/100 stars
      Buy from Supplier

    80
    Bethyl xrn1 antibody
    SARS-CoV-2 disrupts P-body formation. Uninfected Vero E6 TMPRSS2 or HEK293T ACE2 cells and their SARS-CoV-2-infected cells at 24 h postinfection were stained with anti-SARS-CoV-2 nucleocapsid (ab273434 [6H3]) and anti-DDX6 (A300-460A) antibodies. The cells were also stained with anti-SARS-CoV-2 nucleocapsid and either <t>anti-Xrn1</t> (A300-443A) or anti-MOV10 (A301-571A) antibodies. Cells were then stained with donkey anti-rabbit IgG (H+L) Alexa Fluor 488-conjugated secondary antibody and donkey anti-mouse IgG (H+L) Alexa Fluor 594-conjugated secondary antibody. Images were visualized using confocal laser scanning microscopy. The two-color overlay images are also exhibited (Merged). Nuclei were stained with DAPI (blue).
    Xrn1 Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xrn1 antibody/product/Bethyl
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xrn1 antibody - by Bioz Stars, 2022-11
    80/100 stars
      Buy from Supplier

    Image Search Results


    MARF1 and XRN1 use analogous motifs to interact with EDC4. ( A ) Schematic diagram for MARF1 and XRN1 domain structures. ( B ) Phylogenetic alignment of the candidate C-terminal distal region that mediates MARF1-EDC4 interactions and the XRN1 EDC4-binding motif (EBM) in H. sapiens ( Hs ), X. tropicalis ( Xt ), and D. rerio ( Dr ). Residues in blue are conservative substitutions that are analogous in both proteins, residues in red are identical residues between all orthologs for each protein. The (*) represents the C-terminus of the protein and an analogous phenylalanine is indicated with a red arrow located within the SLiM; LAΦNF. ( C and D ) Co-immunoprecipitation experiments performed by incubating HeLa cell extracts expressing the indicated FLAG-tagged proteins and incubated with FLAG antibody. Subsequent western blot analysis was performed to identify the presence of endogenous EDC4. ( E ) Images generated through confocal microscopy of immunofluorescent staining of HeLa cells transfected with FLAG-MARF1 variants. The merged image represents the overlap of the α-FLAG (red) and α-EDC4 (green) signals and scale bars represent 20 μm. White arrows represent P-bodies as defined by foci with strong signal overlap between red and green staining. ( F ) RL-MAML1 activity detected in extracts of transfected HeLa cells expressing the annotated proteins and FL as a transfection normalization control. Histograms represent the mean RL activity detected from three biological replicates, normalized to FL activity and the RL/FL ratio of a catalytically inactive MARF1 (MARF1 ΔNYN ) set to 100. Error bars represent the SEM and statistical significance was calculated using a two-tailed T-test comparing RL activity in the (+) to (-) EDC4 overexpression samples (*** P

    Journal: Nucleic Acids Research

    Article Title: P-bodies directly regulate MARF1-mediated mRNA decay in human cells

    doi: 10.1093/nar/gkac557

    Figure Lengend Snippet: MARF1 and XRN1 use analogous motifs to interact with EDC4. ( A ) Schematic diagram for MARF1 and XRN1 domain structures. ( B ) Phylogenetic alignment of the candidate C-terminal distal region that mediates MARF1-EDC4 interactions and the XRN1 EDC4-binding motif (EBM) in H. sapiens ( Hs ), X. tropicalis ( Xt ), and D. rerio ( Dr ). Residues in blue are conservative substitutions that are analogous in both proteins, residues in red are identical residues between all orthologs for each protein. The (*) represents the C-terminus of the protein and an analogous phenylalanine is indicated with a red arrow located within the SLiM; LAΦNF. ( C and D ) Co-immunoprecipitation experiments performed by incubating HeLa cell extracts expressing the indicated FLAG-tagged proteins and incubated with FLAG antibody. Subsequent western blot analysis was performed to identify the presence of endogenous EDC4. ( E ) Images generated through confocal microscopy of immunofluorescent staining of HeLa cells transfected with FLAG-MARF1 variants. The merged image represents the overlap of the α-FLAG (red) and α-EDC4 (green) signals and scale bars represent 20 μm. White arrows represent P-bodies as defined by foci with strong signal overlap between red and green staining. ( F ) RL-MAML1 activity detected in extracts of transfected HeLa cells expressing the annotated proteins and FL as a transfection normalization control. Histograms represent the mean RL activity detected from three biological replicates, normalized to FL activity and the RL/FL ratio of a catalytically inactive MARF1 (MARF1 ΔNYN ) set to 100. Error bars represent the SEM and statistical significance was calculated using a two-tailed T-test comparing RL activity in the (+) to (-) EDC4 overexpression samples (*** P

    Article Snippet: Antibodies for this study were purchased against HA (Covance), FLAG (Sigma), V5 (Rabbit - Cell Signaling; Mouse - Thermofisher), EDC4 (Bethyl), DDX6 (Bethyl), and XRN1 (Bethyl).

    Techniques: Binding Assay, Immunoprecipitation, Expressing, Incubation, Western Blot, Generated, Confocal Microscopy, Staining, Transfection, Activity Assay, Two Tailed Test, Over Expression

    MARF1 and XRN1 competitively interact with EDC4 in a mutually exclusive manner. ( A ) Schematic diagram representing the reporter assay in ( B ), where the MARF1 C-term or the XRN1 EBM compete with low levels of full-length MARF1 for interacting with endogenous EDC4. ( B ) RL-MAML1 activity detected in extracts of transfected HeLa cells expressing the annotated proteins and FL as a transfection normalization control. Histograms represent the mean RL activity detected from three biological replicates, normalized to FL activity and the RL/FL ratio of a catalytically inactive MARF1 (MARF1 ΔNYN ) set to 100. Error bars represent the SEM and statistical significance was calculated using a two-tailed T-test comparing RL activity to the (-) samples for MARF1 WT samples (** P

    Journal: Nucleic Acids Research

    Article Title: P-bodies directly regulate MARF1-mediated mRNA decay in human cells

    doi: 10.1093/nar/gkac557

    Figure Lengend Snippet: MARF1 and XRN1 competitively interact with EDC4 in a mutually exclusive manner. ( A ) Schematic diagram representing the reporter assay in ( B ), where the MARF1 C-term or the XRN1 EBM compete with low levels of full-length MARF1 for interacting with endogenous EDC4. ( B ) RL-MAML1 activity detected in extracts of transfected HeLa cells expressing the annotated proteins and FL as a transfection normalization control. Histograms represent the mean RL activity detected from three biological replicates, normalized to FL activity and the RL/FL ratio of a catalytically inactive MARF1 (MARF1 ΔNYN ) set to 100. Error bars represent the SEM and statistical significance was calculated using a two-tailed T-test comparing RL activity to the (-) samples for MARF1 WT samples (** P

    Article Snippet: Antibodies for this study were purchased against HA (Covance), FLAG (Sigma), V5 (Rabbit - Cell Signaling; Mouse - Thermofisher), EDC4 (Bethyl), DDX6 (Bethyl), and XRN1 (Bethyl).

    Techniques: Reporter Assay, Activity Assay, Transfection, Expressing, Two Tailed Test

    Apical granules lack enzymes for mRNA degradation. a) Each panel represents a 3D image of confocal Z-stack images of a single MCC in culture. Apically localized granules are highlighted by dashed lines. TNRC6A stained with human Index serum (18033). DCP1A, DDX6 and XRN1 are concentrated in randomly-localized granules (P-bodies). However, DCP1A and DDX6 are undetectable in apically granules. XRN1 are present in small number of apically granules at low levels. b) Co-localization profile of apically-localized or randomly-localized granules (P-bodies) within the same MCC indicating the different concentration of these proteins in these two classes of granules. Percentage of granules with double labeling signal.

    Journal: bioRxiv

    Article Title: A local translation program regulates centriole amplification in the airway epithelium

    doi: 10.1101/2022.01.18.476821

    Figure Lengend Snippet: Apical granules lack enzymes for mRNA degradation. a) Each panel represents a 3D image of confocal Z-stack images of a single MCC in culture. Apically localized granules are highlighted by dashed lines. TNRC6A stained with human Index serum (18033). DCP1A, DDX6 and XRN1 are concentrated in randomly-localized granules (P-bodies). However, DCP1A and DDX6 are undetectable in apically granules. XRN1 are present in small number of apically granules at low levels. b) Co-localization profile of apically-localized or randomly-localized granules (P-bodies) within the same MCC indicating the different concentration of these proteins in these two classes of granules. Percentage of granules with double labeling signal.

    Article Snippet: For IF staining, cryosections or transwell filter of ALI cultures were washed in PBS, and then blocked by 5% donkey serum in PBST (1XPBS, 0.3% Triton X-100) for one hour at RT, followed by incubation with primary antibodies overnight at 4°C (TNRC6A, human index serum, 1:3000; FOXJ1, eBioscience, # 14-9965-82, 1:100; Ac-α-tub, Sigma, # T7451, 1:10,000, or Abcam # ab125356; DCP1A, Abcam, #ab183709, 1:50; DDX6, Cell Signaling Technology, #8988S, 1:20; EDC3, Santa Cruz, # sc-55081, 1:20; EDC4/GE-1, Cell Signaling Technology, #2548S, 1:50; Xrn1, Bethyl Laboratories, #A300-443A, 1:20; γ-tubulin, Abcam, #ab84355, 1:50; EIF3B, Santa Cruz , # sc-16377, 1:50).

    Techniques: Staining, Concentration Assay, Labeling

    SARS-CoV-2 disrupts P-body formation. Uninfected Vero E6 TMPRSS2 or HEK293T ACE2 cells and their SARS-CoV-2-infected cells at 24 h postinfection were stained with anti-SARS-CoV-2 nucleocapsid (ab273434 [6H3]) and anti-DDX6 (A300-460A) antibodies. The cells were also stained with anti-SARS-CoV-2 nucleocapsid and either anti-Xrn1 (A300-443A) or anti-MOV10 (A301-571A) antibodies. Cells were then stained with donkey anti-rabbit IgG (H+L) Alexa Fluor 488-conjugated secondary antibody and donkey anti-mouse IgG (H+L) Alexa Fluor 594-conjugated secondary antibody. Images were visualized using confocal laser scanning microscopy. The two-color overlay images are also exhibited (Merged). Nuclei were stained with DAPI (blue).

    Journal: Journal of Virology

    Article Title: Host Cellular RNA Helicases Regulate SARS-CoV-2 Infection

    doi: 10.1128/jvi.00002-22

    Figure Lengend Snippet: SARS-CoV-2 disrupts P-body formation. Uninfected Vero E6 TMPRSS2 or HEK293T ACE2 cells and their SARS-CoV-2-infected cells at 24 h postinfection were stained with anti-SARS-CoV-2 nucleocapsid (ab273434 [6H3]) and anti-DDX6 (A300-460A) antibodies. The cells were also stained with anti-SARS-CoV-2 nucleocapsid and either anti-Xrn1 (A300-443A) or anti-MOV10 (A301-571A) antibodies. Cells were then stained with donkey anti-rabbit IgG (H+L) Alexa Fluor 488-conjugated secondary antibody and donkey anti-mouse IgG (H+L) Alexa Fluor 594-conjugated secondary antibody. Images were visualized using confocal laser scanning microscopy. The two-color overlay images are also exhibited (Merged). Nuclei were stained with DAPI (blue).

    Article Snippet: I also used anti-DDX6 (A300-460A; Bethyl Lab), anti-XRN1 (A300-443A; Bethyl Lab), anti-G3BP1 (A302-033A; Bethyl), anti-DDX21 (A300-627A; Bethyl Lab), anti-MOV10 (A301-571A; Bethyl Lab), anti-DDX5 (A300-523A; Bethyl Lab), and anti-SARS-CoV-2 nucleocapsid (ab273434 [6H3]; Abcam or GTX632269 [6H3]; GeneTex) antibodies as primary antibodies.

    Techniques: Infection, Staining, Confocal Laser Scanning Microscopy

    Xrn1- cKO mice displayed increased VCO 2 and energy expenditure and constant RER, indicating an inability to utilize fat as an energy source (A and B) (A) respiratory exchange ratio (RER) and (B) energy expenditure of male 6-week-old Xrn1 flox/flox mice (black, n = 5) and Xrn1- cKO mice (red, n = 6) were measured. RER is calculated by VCO 2 /VO 2 . An RER of 1.0 means that carbohydrates are the dominant energy source; 0.7 indicates that fat is the dominant energy source. (C and D) (C) Respiratory exchange ratio (RER) and (D) energy expenditure of male 12-week-old Xrn1 flox/flox mice (black, n = 8) and Xrn1- cKO mice (red, n = 8) were measured. Average values of each parameter in Xrn1 flox/flox mice (white) and Xrn1- cKO mice (red) during light and dark are shown on the right sides.

    Journal: iScience

    Article Title: Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis

    doi: 10.1016/j.isci.2021.103151

    Figure Lengend Snippet: Xrn1- cKO mice displayed increased VCO 2 and energy expenditure and constant RER, indicating an inability to utilize fat as an energy source (A and B) (A) respiratory exchange ratio (RER) and (B) energy expenditure of male 6-week-old Xrn1 flox/flox mice (black, n = 5) and Xrn1- cKO mice (red, n = 6) were measured. RER is calculated by VCO 2 /VO 2 . An RER of 1.0 means that carbohydrates are the dominant energy source; 0.7 indicates that fat is the dominant energy source. (C and D) (C) Respiratory exchange ratio (RER) and (D) energy expenditure of male 12-week-old Xrn1 flox/flox mice (black, n = 8) and Xrn1- cKO mice (red, n = 8) were measured. Average values of each parameter in Xrn1 flox/flox mice (white) and Xrn1- cKO mice (red) during light and dark are shown on the right sides.

    Article Snippet: After blocking in PBS with 0.05% Triton X-100 (PBS-T) containing 5% goat serum for 1 hour at RT, sections were incubated with antibodies against XRN1 (A300-443A; Bethyl Laboratories ), or AgRP (H-003-57; Phoenix Pharmaceuticals) at 4°C overnight.

    Techniques: Mouse Assay

    Xrn1- cKO mice exhibit upregulated appetite and energy homeostasis-related genes in the hypothalamus (A) Quantitative PCR (qPCR) analysis of the indicated mRNA levels in the hypothalamus of 10- to 14-week-old control ( Xrn1 flox/flox : white, n = 5; Camk2a - Cre : gray, n = 10) and Xrn1- cKO mice (red, n = 5). The value of the average of Xrn1 flox/flox mice was set as 1. Data represent means ± SEM. One-way ANOVA, Tukey post hoc test, ∗p

    Journal: iScience

    Article Title: Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis

    doi: 10.1016/j.isci.2021.103151

    Figure Lengend Snippet: Xrn1- cKO mice exhibit upregulated appetite and energy homeostasis-related genes in the hypothalamus (A) Quantitative PCR (qPCR) analysis of the indicated mRNA levels in the hypothalamus of 10- to 14-week-old control ( Xrn1 flox/flox : white, n = 5; Camk2a - Cre : gray, n = 10) and Xrn1- cKO mice (red, n = 5). The value of the average of Xrn1 flox/flox mice was set as 1. Data represent means ± SEM. One-way ANOVA, Tukey post hoc test, ∗p

    Article Snippet: After blocking in PBS with 0.05% Triton X-100 (PBS-T) containing 5% goat serum for 1 hour at RT, sections were incubated with antibodies against XRN1 (A300-443A; Bethyl Laboratories ), or AgRP (H-003-57; Phoenix Pharmaceuticals) at 4°C overnight.

    Techniques: Mouse Assay, Real-time Polymerase Chain Reaction

    Xrn1- cKO mice exhibit aberrant adiposity and liver steatosis (A) Immunoblotting for XRN1, α-Tubulin, and GAPDH in peripheral tissue lysates from Xrn1- cKO and control ( Xrn1 flox/+ ) mice at 12 weeks old. (B) White adipose tissues (eWAT and iWAT), BAT, liver and brain from male 12-week-old Xrn1 flox/flox (left) and Xrn1- cKO (right) littermates. (C) Tissue weights of Xrn1 flox/flox (white, n = 5–8) and Xrn1- cKO (red, n = 3–7) mice at 12 weeks old. Data represent means ± SD. Unpaired Student’s t test, ∗∗p

    Journal: iScience

    Article Title: Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis

    doi: 10.1016/j.isci.2021.103151

    Figure Lengend Snippet: Xrn1- cKO mice exhibit aberrant adiposity and liver steatosis (A) Immunoblotting for XRN1, α-Tubulin, and GAPDH in peripheral tissue lysates from Xrn1- cKO and control ( Xrn1 flox/+ ) mice at 12 weeks old. (B) White adipose tissues (eWAT and iWAT), BAT, liver and brain from male 12-week-old Xrn1 flox/flox (left) and Xrn1- cKO (right) littermates. (C) Tissue weights of Xrn1 flox/flox (white, n = 5–8) and Xrn1- cKO (red, n = 3–7) mice at 12 weeks old. Data represent means ± SD. Unpaired Student’s t test, ∗∗p

    Article Snippet: After blocking in PBS with 0.05% Triton X-100 (PBS-T) containing 5% goat serum for 1 hour at RT, sections were incubated with antibodies against XRN1 (A300-443A; Bethyl Laboratories ), or AgRP (H-003-57; Phoenix Pharmaceuticals) at 4°C overnight.

    Techniques: Mouse Assay

    Xrn1- cKO mice exhibit metabolic disorders with hyperglycemia, hyperinsulinemia, and hyperleptinemia (A) Levels of circulating blood glucose (left), serum insulin (middle) and serum leptin (right) in 5-week male control (white, n = 5–8) and Xrn1 -cKO (red, n = 5–6) mice. (B and C) (C) To test leptin sensitivity, 5-week-old Xrn1 flox/flox (black, n = 5) and Xrn1- cKO (red, n = 5) mice were intraperitoneally administered with leptin (5 μg/g body weight) every day for 5 days (blue arrows), daily body weight (B) and food intake (C) were measured for 11 days. Δbody weight (%) is shown as the percentage of daily weight gain normalized by daily body weight. (D) Fed (left) and fasting (right) blood glucose levels in 5-, 7- and 9-week-old control (black, n = 5) and Xrn1 -cKO (red, n = 5) mice are shown. (E) Intraperitoneal glucose tolerance test (IPGTT) using 5-week-old control (black, n = 5) and Xrn1 -cKO (red, n = 5) mice. Areas under the curves (AUC) of IPGTT are shown on the right side. (F) Insulin tolerance test (ITT) using 5-week-old control (black, n = 5) and Xrn1 -cKO (red, n = 5) mice. Values at 0 min are set as 100%. Areas under the curves (AUC) of ITT are shown on the right side. Data represent means ± SEM. Two-way ANOVA followed by Bonferroni post hoc test for fed and fasting blood glucose, IPGTT and ITT. Unpaired Student’s t test for AUC, ∗p

    Journal: iScience

    Article Title: Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis

    doi: 10.1016/j.isci.2021.103151

    Figure Lengend Snippet: Xrn1- cKO mice exhibit metabolic disorders with hyperglycemia, hyperinsulinemia, and hyperleptinemia (A) Levels of circulating blood glucose (left), serum insulin (middle) and serum leptin (right) in 5-week male control (white, n = 5–8) and Xrn1 -cKO (red, n = 5–6) mice. (B and C) (C) To test leptin sensitivity, 5-week-old Xrn1 flox/flox (black, n = 5) and Xrn1- cKO (red, n = 5) mice were intraperitoneally administered with leptin (5 μg/g body weight) every day for 5 days (blue arrows), daily body weight (B) and food intake (C) were measured for 11 days. Δbody weight (%) is shown as the percentage of daily weight gain normalized by daily body weight. (D) Fed (left) and fasting (right) blood glucose levels in 5-, 7- and 9-week-old control (black, n = 5) and Xrn1 -cKO (red, n = 5) mice are shown. (E) Intraperitoneal glucose tolerance test (IPGTT) using 5-week-old control (black, n = 5) and Xrn1 -cKO (red, n = 5) mice. Areas under the curves (AUC) of IPGTT are shown on the right side. (F) Insulin tolerance test (ITT) using 5-week-old control (black, n = 5) and Xrn1 -cKO (red, n = 5) mice. Values at 0 min are set as 100%. Areas under the curves (AUC) of ITT are shown on the right side. Data represent means ± SEM. Two-way ANOVA followed by Bonferroni post hoc test for fed and fasting blood glucose, IPGTT and ITT. Unpaired Student’s t test for AUC, ∗p

    Article Snippet: After blocking in PBS with 0.05% Triton X-100 (PBS-T) containing 5% goat serum for 1 hour at RT, sections were incubated with antibodies against XRN1 (A300-443A; Bethyl Laboratories ), or AgRP (H-003-57; Phoenix Pharmaceuticals) at 4°C overnight.

    Techniques: Mouse Assay

    Camk2a-Cre -mediated deletion of Xrn1 in brain causes obesity and hyperphagia (A) XRN1, α-Tubulin, and GAPDH in brain lysates from 12-week-old Xrn1- cKO and control ( Xrn1 flox/+ ) mice are shown by western blotting. (B) XRN1 expression (green) in the hypothalamus of 5-week-old male Xrn1 flox/flox and Xrn1- cKO mice is shown by immunofluorescence staining. Nuclei were shown by DAPI staining (blue). (C) 12-week-old male Xrn1 flox/flox and Xrn1- cKO littermates are shown. (D) Body lengths (nose-to-anus) of male 12-week-old Xrn1 flox/flox (white, n = 7) and Xrn1- cKO mice (red, n = 6). (E–J) Growth curve of Xrn1 flox/flox (white circle, n = 10), Camk2a-Cre (white square, n = 10) and Xrn1- cKO male (red triangle, n = 10) (E) and female (H) mice from 3 to 12 weeks old. Data represent means ± SD. One-way ANOVA between Xrn1 flox/flox and Xrn1- cKO mice ∗p

    Journal: iScience

    Article Title: Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis

    doi: 10.1016/j.isci.2021.103151

    Figure Lengend Snippet: Camk2a-Cre -mediated deletion of Xrn1 in brain causes obesity and hyperphagia (A) XRN1, α-Tubulin, and GAPDH in brain lysates from 12-week-old Xrn1- cKO and control ( Xrn1 flox/+ ) mice are shown by western blotting. (B) XRN1 expression (green) in the hypothalamus of 5-week-old male Xrn1 flox/flox and Xrn1- cKO mice is shown by immunofluorescence staining. Nuclei were shown by DAPI staining (blue). (C) 12-week-old male Xrn1 flox/flox and Xrn1- cKO littermates are shown. (D) Body lengths (nose-to-anus) of male 12-week-old Xrn1 flox/flox (white, n = 7) and Xrn1- cKO mice (red, n = 6). (E–J) Growth curve of Xrn1 flox/flox (white circle, n = 10), Camk2a-Cre (white square, n = 10) and Xrn1- cKO male (red triangle, n = 10) (E) and female (H) mice from 3 to 12 weeks old. Data represent means ± SD. One-way ANOVA between Xrn1 flox/flox and Xrn1- cKO mice ∗p

    Article Snippet: After blocking in PBS with 0.05% Triton X-100 (PBS-T) containing 5% goat serum for 1 hour at RT, sections were incubated with antibodies against XRN1 (A300-443A; Bethyl Laboratories ), or AgRP (H-003-57; Phoenix Pharmaceuticals) at 4°C overnight.

    Techniques: Mouse Assay, Western Blot, Expressing, Immunofluorescence, Staining

    A lack of XRN1 in neurons causes increased appetite, decreased energy expenditure, hyperglycemia, and dysfunctions of metabolic hormones Appetite circuits in the hypothalamus and consequent metabolic functions in peripheral tissues such as adipose tissue, pancreas, and liver. The diagram indicates dysregulation of metabolic homeostasis by activated AgRP neurons in Xrn1- cKO hypothalamus. EE: Energy expenditure.

    Journal: iScience

    Article Title: Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis

    doi: 10.1016/j.isci.2021.103151

    Figure Lengend Snippet: A lack of XRN1 in neurons causes increased appetite, decreased energy expenditure, hyperglycemia, and dysfunctions of metabolic hormones Appetite circuits in the hypothalamus and consequent metabolic functions in peripheral tissues such as adipose tissue, pancreas, and liver. The diagram indicates dysregulation of metabolic homeostasis by activated AgRP neurons in Xrn1- cKO hypothalamus. EE: Energy expenditure.

    Article Snippet: After blocking in PBS with 0.05% Triton X-100 (PBS-T) containing 5% goat serum for 1 hour at RT, sections were incubated with antibodies against XRN1 (A300-443A; Bethyl Laboratories ), or AgRP (H-003-57; Phoenix Pharmaceuticals) at 4°C overnight.

    Techniques: