Structured Review

Thermo Fisher xmai
( A ) Southern blot of a 15-kb PCR amplicon (primer pair 3) (, see below) from the distal region of the Rsp locus digested with EagI, <t>HindIII,</t> SstI, and <t>XmaI.</t> We detected bands for all predicted fragments (predictions in boxes). The location of the15-kb
Xmai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xmai/product/Thermo Fisher
Average 92 stars, based on 24 article reviews
Price from $9.99 to $1999.99
xmai - by Bioz Stars, 2020-07
92/100 stars

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1) Product Images from "Single-molecule sequencing resolves the detailed structure of complex satellite DNA loci in Drosophila melanogaster"

Article Title: Single-molecule sequencing resolves the detailed structure of complex satellite DNA loci in Drosophila melanogaster

Journal: Genome Research

doi: 10.1101/gr.213512.116

( A ) Southern blot of a 15-kb PCR amplicon (primer pair 3) (, see below) from the distal region of the Rsp locus digested with EagI, HindIII, SstI, and XmaI. We detected bands for all predicted fragments (predictions in boxes). The location of the15-kb
Figure Legend Snippet: ( A ) Southern blot of a 15-kb PCR amplicon (primer pair 3) (, see below) from the distal region of the Rsp locus digested with EagI, HindIII, SstI, and XmaI. We detected bands for all predicted fragments (predictions in boxes). The location of the15-kb

Techniques Used: Southern Blot, Polymerase Chain Reaction, Amplification

Related Articles

Clone Assay:

Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
Article Snippet: .. This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene. .. Finally, the combined 2.5 kb fragment containing the native and recodonized cdpk1 sequences was cloned between XmaI and AvrII sites of the pHH4-HA plasmid (gift from Dr E. Knuepfer, NIMR), which adds a triple HA tag at the 3′ end followed by a stop codon and the PbDT 3′ UTR and hDHFR drug selection cassette.

Article Title: Imidazopyridazine Inhibitors of Plasmodium falciparum Calcium-Dependent Protein Kinase 1 Also Target Cyclic GMP-Dependent Protein Kinase and Heat Shock Protein 90 To Kill the Parasite at Different Stages of Intracellular Development
Article Snippet: .. Each of these fragments was cloned via XmaI and EcoRI sites into a Geneart vector containing a recodonized gene fragment from bp 436 to 1572 of the P. falciparum cdpk1 (Pfcdpk1 ) open reading frame. .. Together, the native and recodonized cdpk1 sequences were cloned between XmaI and AvrII sites of the pHH4-HA plasmid , which adds a triple hemagglutinin (HA) tag at the 3′ end, followed by a stop codon and the PbDT-3′ UTR (the 3′ untranslated region of the P. berghei dihydrofolate reductase [DHFR]-thymidylate synthase) and human DHFR (hDHFR) drug selection cassette.

In Vitro:

Article Title: Knockdown of Bardet-Biedl Syndrome Gene BBS9/PTHB1 Leads to Cilia Defects
Article Snippet: .. Subcloned cDNAs were linearized with XmaI and used for in vitro synthesis of capped mRNA using mMESSAGE mMACHINE T7 Ultra Kit (Ambion, Applied Biosystems, CA, USA). .. The quality of in vitro synthesized mRNA was checked on Bioanalyser (Agilent Tech, CA, USA) before co-injection with bbs9 -spMO.

Southern Blot:

Article Title: Single-molecule sequencing resolves the detailed structure of complex satellite DNA loci in Drosophila melanogaster
Article Snippet: .. To confirm the locus orientation, we designed PCR primers that could only amplify an ∼15-kb segment of the distal part of the locus found in the PBcR-BLASR assembly (primer pair 3, 5′-CATGTGTGAACAGTGTATTCTG-3′ and 5′-GGCAGGAGTATTAAATCGATCTTC-3′) ( A; ) and confirmed its organization using restriction enzyme digestion with HindIII, EagI, SstI, and XmaI, and Southern blot analysis using a biotinylated Rsp probe and the North2South kit (ThermoFisher #17175) ( A). .. We validated the distal and proximal ends of the locus with a Southern blot analysis (see below) on genomic DNA digested with AccI, EcoRI, FspI, and SstI ( B; ).

Synthesized:

Article Title: Evidence for a cooperative role of gelatinase A and membrane type-1 matrix metalloproteinase during Xenopus laevis development.
Article Snippet: .. The mRNA encoding MT1-MMP-FLAG, MT1-MMP-Myc, GelA-FLAG and their mutants were synthesized from the plasmid DNA constructs linearized with XmaI by using mMESSAGE mMACHINE (Ambion) according to the manufacture’s instructions. .. Fertilized Xenopus eggs were dejellied in 0.1x Marc’s modified ringers (0.1x MMR: 10 mM NaCl, 0.2 mM KCl, 0.1 mM MgCl2 , 0.2 mM CaCl2 and 0.5 mM HEPES, pH 7.6) containing 2% L-cysteine, washed 5 times with 0.1x MMR and kept in 0.1x MMR-6% Ficoll 400.

Ligation:

Article Title: Cold-induced urticarial autoinflammatory syndrome related to factor XII activation
Article Snippet: .. First, the N and M segments were digested with XmaI and subsequently, fused by ligation with T4 ligase (Thermo Fisher Scientific). ..

Construct:

Article Title: Evidence for a cooperative role of gelatinase A and membrane type-1 matrix metalloproteinase during Xenopus laevis development.
Article Snippet: .. The mRNA encoding MT1-MMP-FLAG, MT1-MMP-Myc, GelA-FLAG and their mutants were synthesized from the plasmid DNA constructs linearized with XmaI by using mMESSAGE mMACHINE (Ambion) according to the manufacture’s instructions. .. Fertilized Xenopus eggs were dejellied in 0.1x Marc’s modified ringers (0.1x MMR: 10 mM NaCl, 0.2 mM KCl, 0.1 mM MgCl2 , 0.2 mM CaCl2 and 0.5 mM HEPES, pH 7.6) containing 2% L-cysteine, washed 5 times with 0.1x MMR and kept in 0.1x MMR-6% Ficoll 400.

Polymerase Chain Reaction:

Article Title: Single-molecule sequencing resolves the detailed structure of complex satellite DNA loci in Drosophila melanogaster
Article Snippet: .. To confirm the locus orientation, we designed PCR primers that could only amplify an ∼15-kb segment of the distal part of the locus found in the PBcR-BLASR assembly (primer pair 3, 5′-CATGTGTGAACAGTGTATTCTG-3′ and 5′-GGCAGGAGTATTAAATCGATCTTC-3′) ( A; ) and confirmed its organization using restriction enzyme digestion with HindIII, EagI, SstI, and XmaI, and Southern blot analysis using a biotinylated Rsp probe and the North2South kit (ThermoFisher #17175) ( A). .. We validated the distal and proximal ends of the locus with a Southern blot analysis (see below) on genomic DNA digested with AccI, EcoRI, FspI, and SstI ( B; ).

Plasmid Preparation:

Article Title: Evidence for a cooperative role of gelatinase A and membrane type-1 matrix metalloproteinase during Xenopus laevis development.
Article Snippet: .. The mRNA encoding MT1-MMP-FLAG, MT1-MMP-Myc, GelA-FLAG and their mutants were synthesized from the plasmid DNA constructs linearized with XmaI by using mMESSAGE mMACHINE (Ambion) according to the manufacture’s instructions. .. Fertilized Xenopus eggs were dejellied in 0.1x Marc’s modified ringers (0.1x MMR: 10 mM NaCl, 0.2 mM KCl, 0.1 mM MgCl2 , 0.2 mM CaCl2 and 0.5 mM HEPES, pH 7.6) containing 2% L-cysteine, washed 5 times with 0.1x MMR and kept in 0.1x MMR-6% Ficoll 400.

Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
Article Snippet: .. This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene. .. Finally, the combined 2.5 kb fragment containing the native and recodonized cdpk1 sequences was cloned between XmaI and AvrII sites of the pHH4-HA plasmid (gift from Dr E. Knuepfer, NIMR), which adds a triple HA tag at the 3′ end followed by a stop codon and the PbDT 3′ UTR and hDHFR drug selection cassette.

Article Title: Imidazopyridazine Inhibitors of Plasmodium falciparum Calcium-Dependent Protein Kinase 1 Also Target Cyclic GMP-Dependent Protein Kinase and Heat Shock Protein 90 To Kill the Parasite at Different Stages of Intracellular Development
Article Snippet: .. Each of these fragments was cloned via XmaI and EcoRI sites into a Geneart vector containing a recodonized gene fragment from bp 436 to 1572 of the P. falciparum cdpk1 (Pfcdpk1 ) open reading frame. .. Together, the native and recodonized cdpk1 sequences were cloned between XmaI and AvrII sites of the pHH4-HA plasmid , which adds a triple hemagglutinin (HA) tag at the 3′ end, followed by a stop codon and the PbDT-3′ UTR (the 3′ untranslated region of the P. berghei dihydrofolate reductase [DHFR]-thymidylate synthase) and human DHFR (hDHFR) drug selection cassette.

Article Title: Revealing the role of phospholipase C?3 in the regulation of VEGF-induced vascular permeability
Article Snippet: .. Cre mRNA was transcribed from pT3TS-Cre plasmid DNA linearized with XmaI using the mMessage mMachine T3 kit (Ambion). ..

Article Title: tBRD-1 and tBRD-2 regulate expression of genes necessary for spermatid differentiation
Article Snippet: .. The two parts were ligated together into the TOPO-TA vector (Life Technologies) using XmaI and XhoI. .. The constructed final tbrd-2ΔNET was amplified using tbrd-2-Y2H-NdeI-fw /tbrd-2-Y2H-EcoRI-rv .

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    Thermo Fisher cfr 9i
    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and <t>Cfr</t> 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.
    Cfr 9i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfr 9i/product/Thermo Fisher
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    cfr 9i - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    92
    Thermo Fisher xmai
    ( A ) Southern blot of a 15-kb PCR amplicon (primer pair 3) (, see below) from the distal region of the Rsp locus digested with EagI, <t>HindIII,</t> SstI, and <t>XmaI.</t> We detected bands for all predicted fragments (predictions in boxes). The location of the15-kb
    Xmai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xmai/product/Thermo Fisher
    Average 92 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    xmai - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and Cfr 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.

    Journal: Microbial Drug Resistance

    Article Title: Changes in Macrolide Resistance Among Group A Streptococci in Serbia and Clonal Evolution of Resistant Isolates

    doi: 10.1089/mdr.2017.0306

    Figure Lengend Snippet: Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and Cfr 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.

    Article Snippet: Briefly, chromosomal DNAs of MRGAS isolates expressing the MLS phenotype were digested with the Sma I (10 U) restriction enzyme (Thermo Scientific), (run time: 18 hr, switching intervals: 5–35 sec, angle: 120°, voltage: 6 V, and cooling temperature: 14°C), while DNAs of strains with an M phenotype were treated with the Cfr 9I (30 U) restriction enzyme (Thermo Scientific), (run time: 18 hr, switching intervals: 5–35 sec, angle: 120°, voltage: 6 V, and cooling temperature: 14°C).

    Techniques: Pulsed-Field Gel, Electrophoresis

    ( A ) Southern blot of a 15-kb PCR amplicon (primer pair 3) (, see below) from the distal region of the Rsp locus digested with EagI, HindIII, SstI, and XmaI. We detected bands for all predicted fragments (predictions in boxes). The location of the15-kb

    Journal: Genome Research

    Article Title: Single-molecule sequencing resolves the detailed structure of complex satellite DNA loci in Drosophila melanogaster

    doi: 10.1101/gr.213512.116

    Figure Lengend Snippet: ( A ) Southern blot of a 15-kb PCR amplicon (primer pair 3) (, see below) from the distal region of the Rsp locus digested with EagI, HindIII, SstI, and XmaI. We detected bands for all predicted fragments (predictions in boxes). The location of the15-kb

    Article Snippet: To confirm the locus orientation, we designed PCR primers that could only amplify an ∼15-kb segment of the distal part of the locus found in the PBcR-BLASR assembly (primer pair 3, 5′-CATGTGTGAACAGTGTATTCTG-3′ and 5′-GGCAGGAGTATTAAATCGATCTTC-3′) ( A; ) and confirmed its organization using restriction enzyme digestion with HindIII, EagI, SstI, and XmaI, and Southern blot analysis using a biotinylated Rsp probe and the North2South kit (ThermoFisher #17175) ( A).

    Techniques: Southern Blot, Polymerase Chain Reaction, Amplification