xmai  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Name:
    Cfr9I (XmaI)
    Description:
    5'  C ↓C  C  G  G  G   3' 3'  G  G  G  C  C ↑C   5' Thermo Scientific Cfr9I (XmaI) restriction enzyme recognizes C^CCGGG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: TspMI, XmaCI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Features• Superior quality—stringent quality control and industry leading manufacturing process• Convenient color-coded Five Buffer System• Includes universal Tango buffer for double-digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism (RFLP)• SNPNote: To achieve complete digestion of substrate with Cfr9I, the concentration of DNA should be no less than 50 µg/mL in the reaction buffer.For methylation sensitivity, refer to product specifications.
    Catalog Number:
    ER0171
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Size:
    300 units
    Category:
    Proteins, Enzymes, & Peptides, PCR & Cloning Enzymes, Restriction Enzymes
    Score:
    85
    Buy from Supplier


    Structured Review

    Thermo Fisher xmai
    5'  C ↓C  C  G  G  G   3' 3'  G  G  G  C  C ↑C   5' Thermo Scientific Cfr9I (XmaI) restriction enzyme recognizes C^CCGGG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: TspMI, XmaCI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Features• Superior quality—stringent quality control and industry leading manufacturing process• Convenient color-coded Five Buffer System• Includes universal Tango buffer for double-digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism (RFLP)• SNPNote: To achieve complete digestion of substrate with Cfr9I, the concentration of DNA should be no less than 50 µg/mL in the reaction buffer.For methylation sensitivity, refer to product specifications.
    https://www.bioz.com/result/xmai/product/Thermo Fisher
    Average 96 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    xmai - by Bioz Stars, 2019-12
    96/100 stars

    Images

    Related Articles

    DNA Extraction:

    Article Title: Human Adenovirus 52 Uses Sialic Acid-containing Glycoproteins and the Coxsackie and Adenovirus Receptor for Binding to Target Cells
    Article Snippet: DNA isolation from HAdV-52 virions was performed by using the Blood & Cell Culture DNA Mini kit (Qiagen Nordic). .. Fragments were then cloned into a pQE30Xa expression vector encoding an N-terminal His-tag (Qiagen) using restriction sites for BamHI and XmaI (Fermentas, ThermoFisher Scientific).

    Clone Assay:

    Article Title: Decoy Receptor Interactions as Novel Drug Targets against EKC-Causing Human Adenovirus
    Article Snippet: Cloning, expression, and purification of fiber knobs (FKs) were carried out as described previously [ ]. .. Briefly, for the production of His-tagged FKs, HAdV-C5 and HAdV-D37 FK genes were cloned into a pQE30-Xa expression vector encoding a His-tag (N-terminal) using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA). .. For the production of GST-tagged 37FKs, HAdV-D37 FK gene was cloned into a pGEX-6P expression vector encoding a GST-tag (N-terminal) using restriction sites for NcoI and XhoI (Thermo Scientific, Waltham, MA, USA).

    Article Title: Sulfated Glycosaminoglycans as Viral Decoy Receptors for Human Adenovirus Type 37
    Article Snippet: Cloning, expression, and purification of fiber knobs were performed as described previously [ ]. .. Briefly, the fiber knob genes of HAdV-C5 and HAdV-D37 were cloned into a pQE30-Xa expression vector encoding an N-terminal His-tag using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA). .. GST-tagged HAdV-D37 fiber knob was produced as following; HAdV-D37 fiber knob gene was cloned into a pGEX-6P expression vector encoding an N-terminal GST-tag using restriction sites for NcoI and XhoI (Thermo Scientific).

    Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
    Article Snippet: A region of homology to facilitate integration of the plasmid via single crossover homologous recombination was amplified from P. falciparum 3D7 genomic DNA: 194 bp upstream of the ATG to base pair 435 of the open reading frame using primers 1 and 2. .. This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene. .. Finally, the combined 2.5 kb fragment containing the native and recodonized cdpk1 sequences was cloned between XmaI and AvrII sites of the pHH4-HA plasmid (gift from Dr E. Knuepfer, NIMR), which adds a triple HA tag at the 3′ end followed by a stop codon and the PbDT 3′ UTR and hDHFR drug selection cassette.

    Article Title: Mapping the Effect of Gly Mutations in Collagen on α2β1 Integrin Binding
    Article Snippet: Paragraph title: Design and Cloning of Constructs ... Oligonucleotides encoding the sequence GARGERGFPGERGVQGPP from the human collagen α1 chain were designed with XmaI and ApaI sticky ends and synthesized (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Genetic Characterization of the Galactitol Utilization Pathway of Salmonella enterica Serovar Typhimurium
    Article Snippet: Typhimurium 4/74 by PCR using the primers listed in Table S1. .. The fragments were then cloned via SacI and XmaI (Fermentas) upstream of the promoterless luxCDABE genes into the multiple-cloning site of pUTs- lux (Cmr ). .. For cloning putative promoters into the suicide vector pUTs- gfp (Cmr ), NotI and KpnI (Fermentas) were used.

    Article Title: Imidazopyridazine Inhibitors of Plasmodium falciparum Calcium-Dependent Protein Kinase 1 Also Target Cyclic GMP-Dependent Protein Kinase and Heat Shock Protein 90 To Kill the Parasite at Different Stages of Intracellular Development
    Article Snippet: Briefly, a region of homology to facilitate integration of the plasmid via single-crossover homologous recombination was amplified from P. falciparum 3D7 genomic DNA at 194 bp upstream of the ATG to bp 435 of the open reading frame using primers 1 and 2 for the wild-type (WT) version and primers 1 and 3 for the glycine version. .. Each of these fragments was cloned via XmaI and EcoRI sites into a Geneart vector containing a recodonized gene fragment from bp 436 to 1572 of the P. falciparum cdpk1 (Pfcdpk1 ) open reading frame. .. Together, the native and recodonized cdpk1 sequences were cloned between XmaI and AvrII sites of the pHH4-HA plasmid , which adds a triple hemagglutinin (HA) tag at the 3′ end, followed by a stop codon and the PbDT-3′ UTR (the 3′ untranslated region of the P. berghei dihydrofolate reductase [DHFR]-thymidylate synthase) and human DHFR (hDHFR) drug selection cassette.

    Article Title: Human Adenovirus 52 Uses Sialic Acid-containing Glycoproteins and the Coxsackie and Adenovirus Receptor for Binding to Target Cells
    Article Snippet: DNA fragments encoding HAdV-52 long fiber knob (52LFK) and HAdV-52 short fiber knob (52SFK) were amplified by polymerase chain reaction (PCR) using KOD Hot Start (Novagen, Merck) and the following primers (DNA Technology): 52LFK forward (5´-aaaaggatccggaaacatagctgtttctcct), reverse (5´-aaaacccgggcggaggaagccttactgtgcgtgt), 52SFK forward (5´-aaaaggatccaggtttaacagcagt-ggagcc), reverse (5´-aaaacccgggagggttttattgttcggtaatgtagca). .. Fragments were then cloned into a pQE30Xa expression vector encoding an N-terminal His-tag (Qiagen) using restriction sites for BamHI and XmaI (Fermentas, ThermoFisher Scientific). .. All constructs were confirmed by sequencing (Eurofins MWG Operon).

    Transfection:

    Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
    Article Snippet: This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene. .. Finally, the combined 2.5 kb fragment containing the native and recodonized cdpk1 sequences was cloned between XmaI and AvrII sites of the pHH4-HA plasmid (gift from Dr E. Knuepfer, NIMR), which adds a triple HA tag at the 3′ end followed by a stop codon and the PbDT 3′ UTR and hDHFR drug selection cassette.

    Article Title: Imidazopyridazine Inhibitors of Plasmodium falciparum Calcium-Dependent Protein Kinase 1 Also Target Cyclic GMP-Dependent Protein Kinase and Heat Shock Protein 90 To Kill the Parasite at Different Stages of Intracellular Development
    Article Snippet: Each of these fragments was cloned via XmaI and EcoRI sites into a Geneart vector containing a recodonized gene fragment from bp 436 to 1572 of the P. falciparum cdpk1 (Pfcdpk1 ) open reading frame. .. Each of these fragments was cloned via XmaI and EcoRI sites into a Geneart vector containing a recodonized gene fragment from bp 436 to 1572 of the P. falciparum cdpk1 (Pfcdpk1 ) open reading frame.

    Amplification:

    Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
    Article Snippet: A region of homology to facilitate integration of the plasmid via single crossover homologous recombination was amplified from P. falciparum 3D7 genomic DNA: 194 bp upstream of the ATG to base pair 435 of the open reading frame using primers 1 and 2. .. This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene.

    Article Title: Genetic Characterization of the Galactitol Utilization Pathway of Salmonella enterica Serovar Typhimurium
    Article Snippet: Putative promoter regions spanning approximately 300 bp upstream of the start codons of gatY (STM3253), gatZ (STM3257), gatR (STM3262), and argS (STM1909) were amplified from chromosomal DNA of S . .. The fragments were then cloned via SacI and XmaI (Fermentas) upstream of the promoterless luxCDABE genes into the multiple-cloning site of pUTs- lux (Cmr ).

    Article Title: Imidazopyridazine Inhibitors of Plasmodium falciparum Calcium-Dependent Protein Kinase 1 Also Target Cyclic GMP-Dependent Protein Kinase and Heat Shock Protein 90 To Kill the Parasite at Different Stages of Intracellular Development
    Article Snippet: Briefly, a region of homology to facilitate integration of the plasmid via single-crossover homologous recombination was amplified from P. falciparum 3D7 genomic DNA at 194 bp upstream of the ATG to bp 435 of the open reading frame using primers 1 and 2 for the wild-type (WT) version and primers 1 and 3 for the glycine version. .. Each of these fragments was cloned via XmaI and EcoRI sites into a Geneart vector containing a recodonized gene fragment from bp 436 to 1572 of the P. falciparum cdpk1 (Pfcdpk1 ) open reading frame.

    Article Title: Human Adenovirus 52 Uses Sialic Acid-containing Glycoproteins and the Coxsackie and Adenovirus Receptor for Binding to Target Cells
    Article Snippet: DNA fragments encoding HAdV-52 long fiber knob (52LFK) and HAdV-52 short fiber knob (52SFK) were amplified by polymerase chain reaction (PCR) using KOD Hot Start (Novagen, Merck) and the following primers (DNA Technology): 52LFK forward (5´-aaaaggatccggaaacatagctgtttctcct), reverse (5´-aaaacccgggcggaggaagccttactgtgcgtgt), 52SFK forward (5´-aaaaggatccaggtttaacagcagt-ggagcc), reverse (5´-aaaacccgggagggttttattgttcggtaatgtagca). .. Fragments were then cloned into a pQE30Xa expression vector encoding an N-terminal His-tag (Qiagen) using restriction sites for BamHI and XmaI (Fermentas, ThermoFisher Scientific).

    Plasmid Preparation:

    Article Title: Decoy Receptor Interactions as Novel Drug Targets against EKC-Causing Human Adenovirus
    Article Snippet: Cloning, expression, and purification of fiber knobs (FKs) were carried out as described previously [ ]. .. Briefly, for the production of His-tagged FKs, HAdV-C5 and HAdV-D37 FK genes were cloned into a pQE30-Xa expression vector encoding a His-tag (N-terminal) using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA). .. For the production of GST-tagged 37FKs, HAdV-D37 FK gene was cloned into a pGEX-6P expression vector encoding a GST-tag (N-terminal) using restriction sites for NcoI and XhoI (Thermo Scientific, Waltham, MA, USA).

    Article Title: Sulfated Glycosaminoglycans as Viral Decoy Receptors for Human Adenovirus Type 37
    Article Snippet: Cloning, expression, and purification of fiber knobs were performed as described previously [ ]. .. Briefly, the fiber knob genes of HAdV-C5 and HAdV-D37 were cloned into a pQE30-Xa expression vector encoding an N-terminal His-tag using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA). .. GST-tagged HAdV-D37 fiber knob was produced as following; HAdV-D37 fiber knob gene was cloned into a pGEX-6P expression vector encoding an N-terminal GST-tag using restriction sites for NcoI and XhoI (Thermo Scientific).

    Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
    Article Snippet: A region of homology to facilitate integration of the plasmid via single crossover homologous recombination was amplified from P. falciparum 3D7 genomic DNA: 194 bp upstream of the ATG to base pair 435 of the open reading frame using primers 1 and 2. .. This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene. .. Finally, the combined 2.5 kb fragment containing the native and recodonized cdpk1 sequences was cloned between XmaI and AvrII sites of the pHH4-HA plasmid (gift from Dr E. Knuepfer, NIMR), which adds a triple HA tag at the 3′ end followed by a stop codon and the PbDT 3′ UTR and hDHFR drug selection cassette.

    Article Title: Mapping the Effect of Gly Mutations in Collagen on α2β1 Integrin Binding
    Article Snippet: The DNA sequence of bacterial collagen Scl2.28 was previously codon-optimized and inserted into the pCold-III vector (Takara Bio Inc., Japan) ( ). .. Oligonucleotides encoding the sequence GARGERGFPGERGVQGPP from the human collagen α1 chain were designed with XmaI and ApaI sticky ends and synthesized (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Imidazopyridazine Inhibitors of Plasmodium falciparum Calcium-Dependent Protein Kinase 1 Also Target Cyclic GMP-Dependent Protein Kinase and Heat Shock Protein 90 To Kill the Parasite at Different Stages of Intracellular Development
    Article Snippet: Briefly, a region of homology to facilitate integration of the plasmid via single-crossover homologous recombination was amplified from P. falciparum 3D7 genomic DNA at 194 bp upstream of the ATG to bp 435 of the open reading frame using primers 1 and 2 for the wild-type (WT) version and primers 1 and 3 for the glycine version. .. Each of these fragments was cloned via XmaI and EcoRI sites into a Geneart vector containing a recodonized gene fragment from bp 436 to 1572 of the P. falciparum cdpk1 (Pfcdpk1 ) open reading frame. .. Together, the native and recodonized cdpk1 sequences were cloned between XmaI and AvrII sites of the pHH4-HA plasmid , which adds a triple hemagglutinin (HA) tag at the 3′ end, followed by a stop codon and the PbDT-3′ UTR (the 3′ untranslated region of the P. berghei dihydrofolate reductase [DHFR]-thymidylate synthase) and human DHFR (hDHFR) drug selection cassette.

    Article Title: Human Adenovirus 52 Uses Sialic Acid-containing Glycoproteins and the Coxsackie and Adenovirus Receptor for Binding to Target Cells
    Article Snippet: DNA fragments encoding HAdV-52 long fiber knob (52LFK) and HAdV-52 short fiber knob (52SFK) were amplified by polymerase chain reaction (PCR) using KOD Hot Start (Novagen, Merck) and the following primers (DNA Technology): 52LFK forward (5´-aaaaggatccggaaacatagctgtttctcct), reverse (5´-aaaacccgggcggaggaagccttactgtgcgtgt), 52SFK forward (5´-aaaaggatccaggtttaacagcagt-ggagcc), reverse (5´-aaaacccgggagggttttattgttcggtaatgtagca). .. Fragments were then cloned into a pQE30Xa expression vector encoding an N-terminal His-tag (Qiagen) using restriction sites for BamHI and XmaI (Fermentas, ThermoFisher Scientific). .. All constructs were confirmed by sequencing (Eurofins MWG Operon).

    Synthesized:

    Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
    Article Snippet: A plasmid containing a recodonized version of base pairs 436–1,572 of the pfcdpk1 ORF between XmaI (5′) and AvrII (3′) cloning sites was synthesized by GeneArt. .. This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene.

    Article Title: Mapping the Effect of Gly Mutations in Collagen on α2β1 Integrin Binding
    Article Snippet: The DNA sequence of bacterial collagen Scl2.28 was previously codon-optimized and inserted into the pCold-III vector (Takara Bio Inc., Japan) ( ). .. Oligonucleotides encoding the sequence GARGERGFPGERGVQGPP from the human collagen α1 chain were designed with XmaI and ApaI sticky ends and synthesized (Thermo Fisher Scientific, Waltham, MA). .. Annealed dsDNA was cloned into pCold vector containing the Scl2.28 sequence that was previously digested by XmaI and ApaI.

    Mutagenesis:

    Article Title: Imidazopyridazine Inhibitors of Plasmodium falciparum Calcium-Dependent Protein Kinase 1 Also Target Cyclic GMP-Dependent Protein Kinase and Heat Shock Protein 90 To Kill the Parasite at Different Stages of Intracellular Development
    Article Snippet: Paragraph title: Generation and screening of parasites expressing gatekeeper mutant CDPK1. ... Each of these fragments was cloned via XmaI and EcoRI sites into a Geneart vector containing a recodonized gene fragment from bp 436 to 1572 of the P. falciparum cdpk1 (Pfcdpk1 ) open reading frame.

    Conjugation Assay:

    Article Title: Genetic Characterization of the Galactitol Utilization Pathway of Salmonella enterica Serovar Typhimurium
    Article Snippet: The fragments were then cloned via SacI and XmaI (Fermentas) upstream of the promoterless luxCDABE genes into the multiple-cloning site of pUTs- lux (Cmr ). .. After transformation of E. coli S17.1 cells, plasmids containing the correct transcriptional lux-gfp fusions were verified by PCR.

    Construct:

    Article Title: Decoy Receptor Interactions as Novel Drug Targets against EKC-Causing Human Adenovirus
    Article Snippet: Briefly, for the production of His-tagged FKs, HAdV-C5 and HAdV-D37 FK genes were cloned into a pQE30-Xa expression vector encoding a His-tag (N-terminal) using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA). .. For the production of GST-tagged 37FKs, HAdV-D37 FK gene was cloned into a pGEX-6P expression vector encoding a GST-tag (N-terminal) using restriction sites for NcoI and XhoI (Thermo Scientific, Waltham, MA, USA).

    Article Title: Sulfated Glycosaminoglycans as Viral Decoy Receptors for Human Adenovirus Type 37
    Article Snippet: Briefly, the fiber knob genes of HAdV-C5 and HAdV-D37 were cloned into a pQE30-Xa expression vector encoding an N-terminal His-tag using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA). .. GST-tagged HAdV-D37 fiber knob was produced as following; HAdV-D37 fiber knob gene was cloned into a pGEX-6P expression vector encoding an N-terminal GST-tag using restriction sites for NcoI and XhoI (Thermo Scientific).

    Article Title: Mapping the Effect of Gly Mutations in Collagen on α2β1 Integrin Binding
    Article Snippet: Paragraph title: Design and Cloning of Constructs ... Oligonucleotides encoding the sequence GARGERGFPGERGVQGPP from the human collagen α1 chain were designed with XmaI and ApaI sticky ends and synthesized (Thermo Fisher Scientific, Waltham, MA).

    Purification:

    Article Title: Decoy Receptor Interactions as Novel Drug Targets against EKC-Causing Human Adenovirus
    Article Snippet: Paragraph title: 2.2. Cloning, Expression, and Purification of the Fiber Knob ... Briefly, for the production of His-tagged FKs, HAdV-C5 and HAdV-D37 FK genes were cloned into a pQE30-Xa expression vector encoding a His-tag (N-terminal) using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA).

    Article Title: Sulfated Glycosaminoglycans as Viral Decoy Receptors for Human Adenovirus Type 37
    Article Snippet: Paragraph title: 2.3. Cloning, Expression, and Purification of Fiber Knobs ... Briefly, the fiber knob genes of HAdV-C5 and HAdV-D37 were cloned into a pQE30-Xa expression vector encoding an N-terminal His-tag using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA).

    Article Title: Human Adenovirus 52 Uses Sialic Acid-containing Glycoproteins and the Coxsackie and Adenovirus Receptor for Binding to Target Cells
    Article Snippet: Paragraph title: Cloning and purification of fiber knobs ... Fragments were then cloned into a pQE30Xa expression vector encoding an N-terminal His-tag (Qiagen) using restriction sites for BamHI and XmaI (Fermentas, ThermoFisher Scientific).

    Produced:

    Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
    Article Snippet: The synthetic sequence also contained a novel Eco RI site at position 436–441, produced through introduction of synonymous mutations. .. This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene.

    Sequencing:

    Article Title: Decoy Receptor Interactions as Novel Drug Targets against EKC-Causing Human Adenovirus
    Article Snippet: Briefly, for the production of His-tagged FKs, HAdV-C5 and HAdV-D37 FK genes were cloned into a pQE30-Xa expression vector encoding a His-tag (N-terminal) using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA). .. For the production of GST-tagged 37FKs, HAdV-D37 FK gene was cloned into a pGEX-6P expression vector encoding a GST-tag (N-terminal) using restriction sites for NcoI and XhoI (Thermo Scientific, Waltham, MA, USA).

    Article Title: Sulfated Glycosaminoglycans as Viral Decoy Receptors for Human Adenovirus Type 37
    Article Snippet: Briefly, the fiber knob genes of HAdV-C5 and HAdV-D37 were cloned into a pQE30-Xa expression vector encoding an N-terminal His-tag using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA). .. GST-tagged HAdV-D37 fiber knob was produced as following; HAdV-D37 fiber knob gene was cloned into a pGEX-6P expression vector encoding an N-terminal GST-tag using restriction sites for NcoI and XhoI (Thermo Scientific).

    Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
    Article Snippet: The synthetic sequence also contained a novel Eco RI site at position 436–441, produced through introduction of synonymous mutations. .. This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene.

    Article Title: Mapping the Effect of Gly Mutations in Collagen on α2β1 Integrin Binding
    Article Snippet: The DNA sequence of bacterial collagen Scl2.28 was previously codon-optimized and inserted into the pCold-III vector (Takara Bio Inc., Japan) ( ). .. Oligonucleotides encoding the sequence GARGERGFPGERGVQGPP from the human collagen α1 chain were designed with XmaI and ApaI sticky ends and synthesized (Thermo Fisher Scientific, Waltham, MA). .. Annealed dsDNA was cloned into pCold vector containing the Scl2.28 sequence that was previously digested by XmaI and ApaI.

    Generated:

    Article Title: Imidazopyridazine Inhibitors of Plasmodium falciparum Calcium-Dependent Protein Kinase 1 Also Target Cyclic GMP-Dependent Protein Kinase and Heat Shock Protein 90 To Kill the Parasite at Different Stages of Intracellular Development
    Article Snippet: Parasites expressing CDPK1 T145G and CDPK1 T145T were generated as described previously ( ). .. Each of these fragments was cloned via XmaI and EcoRI sites into a Geneart vector containing a recodonized gene fragment from bp 436 to 1572 of the P. falciparum cdpk1 (Pfcdpk1 ) open reading frame.

    Cell Culture:

    Article Title: Human Adenovirus 52 Uses Sialic Acid-containing Glycoproteins and the Coxsackie and Adenovirus Receptor for Binding to Target Cells
    Article Snippet: DNA isolation from HAdV-52 virions was performed by using the Blood & Cell Culture DNA Mini kit (Qiagen Nordic). .. Fragments were then cloned into a pQE30Xa expression vector encoding an N-terminal His-tag (Qiagen) using restriction sites for BamHI and XmaI (Fermentas, ThermoFisher Scientific).

    Expressing:

    Article Title: Decoy Receptor Interactions as Novel Drug Targets against EKC-Causing Human Adenovirus
    Article Snippet: Cloning, expression, and purification of fiber knobs (FKs) were carried out as described previously [ ]. .. Briefly, for the production of His-tagged FKs, HAdV-C5 and HAdV-D37 FK genes were cloned into a pQE30-Xa expression vector encoding a His-tag (N-terminal) using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA). .. For the production of GST-tagged 37FKs, HAdV-D37 FK gene was cloned into a pGEX-6P expression vector encoding a GST-tag (N-terminal) using restriction sites for NcoI and XhoI (Thermo Scientific, Waltham, MA, USA).

    Article Title: Sulfated Glycosaminoglycans as Viral Decoy Receptors for Human Adenovirus Type 37
    Article Snippet: Cloning, expression, and purification of fiber knobs were performed as described previously [ ]. .. Briefly, the fiber knob genes of HAdV-C5 and HAdV-D37 were cloned into a pQE30-Xa expression vector encoding an N-terminal His-tag using restriction sites for BamHI and XmaI (Thermo Scientific, Waltham, MA, USA). .. GST-tagged HAdV-D37 fiber knob was produced as following; HAdV-D37 fiber knob gene was cloned into a pGEX-6P expression vector encoding an N-terminal GST-tag using restriction sites for NcoI and XhoI (Thermo Scientific).

    Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
    Article Snippet: Paragraph title: Generation and screening of parasites expressing CDPK1-HA ... This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene.

    Article Title: Imidazopyridazine Inhibitors of Plasmodium falciparum Calcium-Dependent Protein Kinase 1 Also Target Cyclic GMP-Dependent Protein Kinase and Heat Shock Protein 90 To Kill the Parasite at Different Stages of Intracellular Development
    Article Snippet: Paragraph title: Generation and screening of parasites expressing gatekeeper mutant CDPK1. ... Each of these fragments was cloned via XmaI and EcoRI sites into a Geneart vector containing a recodonized gene fragment from bp 436 to 1572 of the P. falciparum cdpk1 (Pfcdpk1 ) open reading frame.

    Article Title: Human Adenovirus 52 Uses Sialic Acid-containing Glycoproteins and the Coxsackie and Adenovirus Receptor for Binding to Target Cells
    Article Snippet: DNA fragments encoding HAdV-52 long fiber knob (52LFK) and HAdV-52 short fiber knob (52SFK) were amplified by polymerase chain reaction (PCR) using KOD Hot Start (Novagen, Merck) and the following primers (DNA Technology): 52LFK forward (5´-aaaaggatccggaaacatagctgtttctcct), reverse (5´-aaaacccgggcggaggaagccttactgtgcgtgt), 52SFK forward (5´-aaaaggatccaggtttaacagcagt-ggagcc), reverse (5´-aaaacccgggagggttttattgttcggtaatgtagca). .. Fragments were then cloned into a pQE30Xa expression vector encoding an N-terminal His-tag (Qiagen) using restriction sites for BamHI and XmaI (Fermentas, ThermoFisher Scientific). .. All constructs were confirmed by sequencing (Eurofins MWG Operon).

    Polymerase Chain Reaction:

    Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
    Article Snippet: This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene. .. They were maintained under drug pressure (25 nM WR99210) until resistant parasites emerged, cycled on/off drug and cloned by limiting dilution.

    Article Title: Genetic Characterization of the Galactitol Utilization Pathway of Salmonella enterica Serovar Typhimurium
    Article Snippet: Typhimurium 4/74 by PCR using the primers listed in Table S1. .. The fragments were then cloned via SacI and XmaI (Fermentas) upstream of the promoterless luxCDABE genes into the multiple-cloning site of pUTs- lux (Cmr ).

    Article Title: Human Adenovirus 52 Uses Sialic Acid-containing Glycoproteins and the Coxsackie and Adenovirus Receptor for Binding to Target Cells
    Article Snippet: DNA fragments encoding HAdV-52 long fiber knob (52LFK) and HAdV-52 short fiber knob (52SFK) were amplified by polymerase chain reaction (PCR) using KOD Hot Start (Novagen, Merck) and the following primers (DNA Technology): 52LFK forward (5´-aaaaggatccggaaacatagctgtttctcct), reverse (5´-aaaacccgggcggaggaagccttactgtgcgtgt), 52SFK forward (5´-aaaaggatccaggtttaacagcagt-ggagcc), reverse (5´-aaaacccgggagggttttattgttcggtaatgtagca). .. Fragments were then cloned into a pQE30Xa expression vector encoding an N-terminal His-tag (Qiagen) using restriction sites for BamHI and XmaI (Fermentas, ThermoFisher Scientific).

    Western Blot:

    Article Title: Human Adenovirus 52 Uses Sialic Acid-containing Glycoproteins and the Coxsackie and Adenovirus Receptor for Binding to Target Cells
    Article Snippet: Fragments were then cloned into a pQE30Xa expression vector encoding an N-terminal His-tag (Qiagen) using restriction sites for BamHI and XmaI (Fermentas, ThermoFisher Scientific). .. Proteins were expressed in Escherichia coli (strain M15) and purified with Ni-NTA agarose beads according to protocol from the supplier (Qiagen).

    Transformation Assay:

    Article Title: Genetic Characterization of the Galactitol Utilization Pathway of Salmonella enterica Serovar Typhimurium
    Article Snippet: The fragments were then cloned via SacI and XmaI (Fermentas) upstream of the promoterless luxCDABE genes into the multiple-cloning site of pUTs- lux (Cmr ). .. For cloning putative promoters into the suicide vector pUTs- gfp (Cmr ), NotI and KpnI (Fermentas) were used.

    Homologous Recombination:

    Article Title: Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion
    Article Snippet: A region of homology to facilitate integration of the plasmid via single crossover homologous recombination was amplified from P. falciparum 3D7 genomic DNA: 194 bp upstream of the ATG to base pair 435 of the open reading frame using primers 1 and 2. .. This fragment was cloned via XmaI and EcoRI sites into the GeneArt vector containing the recodonized gene.

    Article Title: Imidazopyridazine Inhibitors of Plasmodium falciparum Calcium-Dependent Protein Kinase 1 Also Target Cyclic GMP-Dependent Protein Kinase and Heat Shock Protein 90 To Kill the Parasite at Different Stages of Intracellular Development
    Article Snippet: Briefly, a region of homology to facilitate integration of the plasmid via single-crossover homologous recombination was amplified from P. falciparum 3D7 genomic DNA at 194 bp upstream of the ATG to bp 435 of the open reading frame using primers 1 and 2 for the wild-type (WT) version and primers 1 and 3 for the glycine version. .. Each of these fragments was cloned via XmaI and EcoRI sites into a Geneart vector containing a recodonized gene fragment from bp 436 to 1572 of the P. falciparum cdpk1 (Pfcdpk1 ) open reading frame.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Thermo Fisher xmai
    Xmai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xmai/product/Thermo Fisher
    Average 96 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    xmai - by Bioz Stars, 2019-12
    96/100 stars
      Buy from Supplier

    Image Search Results