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TaKaRa xmai
Xmai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xmai/product/TaKaRa
Average 95 stars, based on 8 article reviews
Price from $9.99 to $1999.99
xmai - by Bioz Stars, 2020-07
95/100 stars

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Related Articles

Clone Assay:

Article Title: Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase
Article Snippet: .. After the unidirectionally reassembled DNA fragments were purified, they were blunt-ended on both ends with T4 DNA polymerase or S1 nuclease and then sticky-ended on the 3′ end with Sal I. Fragments in the range of about 500 to 960 bp were isolated by preparative agarose gel electrophoresis and cloned into pSTV28. .. As shown below, the treatment of T4 DNA polymerase and the subsequent restriction digestion guaranteed that the randomly truncated and shuffled DNA fragments having exclusively MURA primer sequences at their 3′ end (i.e., fragments reassembled together with MURA primer) could be correctly ligated into 5′ blunt- and 3′ sticky-ended vector (i.e., Sma I- and Sal I-digested pSTV28).

Article Title: A novel and simple method for construction of recombinant adenoviruses
Article Snippet: .. Cloning the foreign genes gfp and man into the donor plasmid using restriction enzyme Bsu36I and T4 DNA polymerase The gfp gene was amplified from pEGFP-1 (Clontech) by PCR. ..

Amplification:

Article Title: A novel and simple method for construction of recombinant adenoviruses
Article Snippet: .. Cloning the foreign genes gfp and man into the donor plasmid using restriction enzyme Bsu36I and T4 DNA polymerase The gfp gene was amplified from pEGFP-1 (Clontech) by PCR. ..

Agarose Gel Electrophoresis:

Article Title: Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase
Article Snippet: .. After the unidirectionally reassembled DNA fragments were purified, they were blunt-ended on both ends with T4 DNA polymerase or S1 nuclease and then sticky-ended on the 3′ end with Sal I. Fragments in the range of about 500 to 960 bp were isolated by preparative agarose gel electrophoresis and cloned into pSTV28. .. As shown below, the treatment of T4 DNA polymerase and the subsequent restriction digestion guaranteed that the randomly truncated and shuffled DNA fragments having exclusively MURA primer sequences at their 3′ end (i.e., fragments reassembled together with MURA primer) could be correctly ligated into 5′ blunt- and 3′ sticky-ended vector (i.e., Sma I- and Sal I-digested pSTV28).

Isolation:

Article Title: Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase
Article Snippet: .. After the unidirectionally reassembled DNA fragments were purified, they were blunt-ended on both ends with T4 DNA polymerase or S1 nuclease and then sticky-ended on the 3′ end with Sal I. Fragments in the range of about 500 to 960 bp were isolated by preparative agarose gel electrophoresis and cloned into pSTV28. .. As shown below, the treatment of T4 DNA polymerase and the subsequent restriction digestion guaranteed that the randomly truncated and shuffled DNA fragments having exclusively MURA primer sequences at their 3′ end (i.e., fragments reassembled together with MURA primer) could be correctly ligated into 5′ blunt- and 3′ sticky-ended vector (i.e., Sma I- and Sal I-digested pSTV28).

Generated:

Article Title: Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase
Article Snippet: .. When the plasmids from randomly selected members were digested with Eco RI and Sal I, we determined that the MURA products were correctly introduced in the form of 5′ blunt- and 3′ sticky-ended fragments, which had been generated by treating T4 DNA polymerase and restriction enzyme Sal I. ..

Purification:

Article Title: Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase
Article Snippet: .. The MURA reaction was performed with an automatic thermal cycler (Ericomp, San Diego, Calif.) for 30 cycles, with each cycle consisting of 94°C for 60 s, 54 to 62°C for 40 s, and 72°C for 60 s. The MURA products were purified with a Qiaquick PCR purification kit (Qiagen) and then treated with 1 U of T4 DNA polymerase (Takara) in 40 μl of the manufacturer's 1X buffer and 0.17 mM each dNTP for 10 min at 37°C or with 10 U of S1 nuclease (Takara) in 40 μl of the manufacturer's 1X buffer for 10 min at 25°C. .. After being purified with a Qiaquick PCR purification kit, the blunt-ended MURA products were digested with Sal I for the purpose of converting the blunt ends to sticky ends at the 3′ ends of the MURA products.

Article Title: Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase
Article Snippet: .. After the unidirectionally reassembled DNA fragments were purified, they were blunt-ended on both ends with T4 DNA polymerase or S1 nuclease and then sticky-ended on the 3′ end with Sal I. Fragments in the range of about 500 to 960 bp were isolated by preparative agarose gel electrophoresis and cloned into pSTV28. .. As shown below, the treatment of T4 DNA polymerase and the subsequent restriction digestion guaranteed that the randomly truncated and shuffled DNA fragments having exclusively MURA primer sequences at their 3′ end (i.e., fragments reassembled together with MURA primer) could be correctly ligated into 5′ blunt- and 3′ sticky-ended vector (i.e., Sma I- and Sal I-digested pSTV28).

Incubation:

Article Title: A sensitive method for the quantification of virion-sense and complementary-sense DNA strands of circular single-stranded DNA viruses
Article Snippet: .. Reaction mixes were denatured (except where indicated) at 95°C for 10 minutes, cooled down to room temperature, and incubated at 37°C for 30 minutes with 1 unit of T4 DNA polymerase. .. Following the reaction, primers were removed using the QIAquick purification kit (QIAGEN, Hamburg, Germany) according to the manufacturer's instructions, and DNA eluted in 50 μl of water.

other:

Article Title: Optimization of single strand DNA incorporation reaction by Moloney murine leukaemia virus reverse transcriptase
Article Snippet: T4 DNA polymerase was purchased from Takara (Japan).

Polymerase Chain Reaction:

Article Title: Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase
Article Snippet: .. The MURA reaction was performed with an automatic thermal cycler (Ericomp, San Diego, Calif.) for 30 cycles, with each cycle consisting of 94°C for 60 s, 54 to 62°C for 40 s, and 72°C for 60 s. The MURA products were purified with a Qiaquick PCR purification kit (Qiagen) and then treated with 1 U of T4 DNA polymerase (Takara) in 40 μl of the manufacturer's 1X buffer and 0.17 mM each dNTP for 10 min at 37°C or with 10 U of S1 nuclease (Takara) in 40 μl of the manufacturer's 1X buffer for 10 min at 25°C. .. After being purified with a Qiaquick PCR purification kit, the blunt-ended MURA products were digested with Sal I for the purpose of converting the blunt ends to sticky ends at the 3′ ends of the MURA products.

Article Title: A novel and simple method for construction of recombinant adenoviruses
Article Snippet: .. Cloning the foreign genes gfp and man into the donor plasmid using restriction enzyme Bsu36I and T4 DNA polymerase The gfp gene was amplified from pEGFP-1 (Clontech) by PCR. ..

Plasmid Preparation:

Article Title: A novel and simple method for construction of recombinant adenoviruses
Article Snippet: .. Cloning the foreign genes gfp and man into the donor plasmid using restriction enzyme Bsu36I and T4 DNA polymerase The gfp gene was amplified from pEGFP-1 (Clontech) by PCR. ..

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  • xmai  (TaKaRa)
    92
    TaKaRa xmai
    Xmai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xmai/product/TaKaRa
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    xmai - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    TaKaRa xhoi xmai digested fragments
    Xhoi Xmai Digested Fragments, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi xmai digested fragments/product/TaKaRa
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xhoi xmai digested fragments - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

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