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TaKaRa xmai
Xmai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xmai/product/TaKaRa
Average 90 stars, based on 3 article reviews
Price from $9.99 to $1999.99
xmai - by Bioz Stars, 2019-12
90/100 stars

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Related Articles

Clone Assay:

Article Title: Male Sterility of an AHAS-Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L.
Article Snippet: We first modified pCAMBIA3301 by inserting an additional CaMV35S promoter into its multiple cloning site by digesting it with EcoRI and SacI (TaKaRa) and ligating with T4 DNA ligase (TaKaRa). .. Then, BnAHAS1544T from pMD19T-BnAHAS1544T was introduced into the modified pCAMBIA3301 by digesting with XmaI and BstEII (TaKaRa) and ligating with T4 DNA ligase.

Article Title: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex
Article Snippet: The p3×FLAG-CMV-10 vector was prepared by first cutting with BamHI, followed by a Klenow fill-in reaction, and then digestion using XbaI. .. The final FLAG-GABPβ construct was generated by the ligation of these two fragments. pTRE-tight-GABPβ was prepared by digesting FLAG-GABPβ with SacI (partial) and XmaI and cloning the FLAG-tagged GABPβ sequence into pTRE-tight (Clontech, Mountain View, CA, USA). .. To create the NRF-1 expression vector, pTRE-tight-GABPβ was digested with BglII and MluI to remove the GABPβ sequence, but retain the FLAG sequence.

Article Title: Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoelii Infection
Article Snippet: Genes of interest were amplified with Phusion Hot Start II polymerase (Finnzymes) according to the manufacturer's instructions by using the Bio-Rad Peltier thermal cycler. .. The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent). .. Bacterial clones were screened for positive transformants by colony PCR with T7 and BgH primers.

Article Title: Potentiation of Amyotrophic Lateral Sclerosis (ALS)-associated TDP-43 Aggregation by the Proteasome-targeting Factor, Ubiquilin 1
Article Snippet: Our findings suggest that UBQLN is a polyubiquitin-TDP-43 cochaperone that mediates the autophagosomal delivery and/or proteasome targeting of TDP-43 aggregates. .. Yeast Two-hybrid Screening and DNA Construction —We screened a human fetal brain cDNA library (Matchmaker™GAL4 Two-Hybrid System3, Clontech) using a full-length TDP-43 cDNA cloned into XmaI and BamHI sites of the bait vector pGBKT7 (Clontech). .. Approximately 7.5 × 106 cDNA clones, which represents over 2-fold coverage of the library, were screened using standard procedures recommended by the Clontech user manual and Saccharomyces cerevisiae strain AH109 as a cotransformation host.

Transfection:

Article Title: Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoelii Infection
Article Snippet: The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent). .. Bacterial clones were screened for positive transformants by colony PCR with T7 and BgH primers.

Amplification:

Article Title: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex
Article Snippet: To generate the FLAG-GABPβ construct, the human GABPβ gene was PCR amplified using pCAGGs-E4TF1-53 (obtained from Hiroshi Handa, [ ]) as the template with the primers specified in Additional File . .. The final FLAG-GABPβ construct was generated by the ligation of these two fragments. pTRE-tight-GABPβ was prepared by digesting FLAG-GABPβ with SacI (partial) and XmaI and cloning the FLAG-tagged GABPβ sequence into pTRE-tight (Clontech, Mountain View, CA, USA).

Article Title: Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoelii Infection
Article Snippet: Genes of interest were amplified with Phusion Hot Start II polymerase (Finnzymes) according to the manufacturer's instructions by using the Bio-Rad Peltier thermal cycler. .. The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent).

Two Hybrid Screening:

Article Title: Potentiation of Amyotrophic Lateral Sclerosis (ALS)-associated TDP-43 Aggregation by the Proteasome-targeting Factor, Ubiquilin 1
Article Snippet: Our findings suggest that UBQLN is a polyubiquitin-TDP-43 cochaperone that mediates the autophagosomal delivery and/or proteasome targeting of TDP-43 aggregates. .. Yeast Two-hybrid Screening and DNA Construction —We screened a human fetal brain cDNA library (Matchmaker™GAL4 Two-Hybrid System3, Clontech) using a full-length TDP-43 cDNA cloned into XmaI and BamHI sites of the bait vector pGBKT7 (Clontech). .. Approximately 7.5 × 106 cDNA clones, which represents over 2-fold coverage of the library, were screened using standard procedures recommended by the Clontech user manual and Saccharomyces cerevisiae strain AH109 as a cotransformation host.

Modification:

Article Title: Male Sterility of an AHAS-Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L.
Article Snippet: We first modified pCAMBIA3301 by inserting an additional CaMV35S promoter into its multiple cloning site by digesting it with EcoRI and SacI (TaKaRa) and ligating with T4 DNA ligase (TaKaRa). .. Then, BnAHAS1544T from pMD19T-BnAHAS1544T was introduced into the modified pCAMBIA3301 by digesting with XmaI and BstEII (TaKaRa) and ligating with T4 DNA ligase. .. The constructed vector CaMV35S:: BnAHAS1544T was confirmed by sequencing, and then transformed into A. thaliana (Col-0) by Agrobacterium tumefaciens -mediated floral-dip method ( ).

Ligation:

Article Title: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex
Article Snippet: The p3×FLAG-CMV-10 vector was prepared by first cutting with BamHI, followed by a Klenow fill-in reaction, and then digestion using XbaI. .. The final FLAG-GABPβ construct was generated by the ligation of these two fragments. pTRE-tight-GABPβ was prepared by digesting FLAG-GABPβ with SacI (partial) and XmaI and cloning the FLAG-tagged GABPβ sequence into pTRE-tight (Clontech, Mountain View, CA, USA). .. To create the NRF-1 expression vector, pTRE-tight-GABPβ was digested with BglII and MluI to remove the GABPβ sequence, but retain the FLAG sequence.

Isolation:

Article Title: Potentiation of Amyotrophic Lateral Sclerosis (ALS)-associated TDP-43 Aggregation by the Proteasome-targeting Factor, Ubiquilin 1
Article Snippet: Yeast Two-hybrid Screening and DNA Construction —We screened a human fetal brain cDNA library (Matchmaker™GAL4 Two-Hybrid System3, Clontech) using a full-length TDP-43 cDNA cloned into XmaI and BamHI sites of the bait vector pGBKT7 (Clontech). .. Individual positive colonies were re-streaked onto SD/-Ade/-His/-Leu/-Trp/X-α-Gal plates and the surviving secondary clones were chosen for further study.

Construct:

Article Title: Male Sterility of an AHAS-Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L.
Article Snippet: To determine whether the allele BnAHAS1544T in K5 was responsible for TBM- resistance or not, we constructed its expression vector CaMV35S:: BnAHAS1544T using pCAMBIA3301 as a backbone. .. Then, BnAHAS1544T from pMD19T-BnAHAS1544T was introduced into the modified pCAMBIA3301 by digesting with XmaI and BstEII (TaKaRa) and ligating with T4 DNA ligase.

Article Title: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex
Article Snippet: The p3×FLAG-CMV-10 vector was prepared by first cutting with BamHI, followed by a Klenow fill-in reaction, and then digestion using XbaI. .. The final FLAG-GABPβ construct was generated by the ligation of these two fragments. pTRE-tight-GABPβ was prepared by digesting FLAG-GABPβ with SacI (partial) and XmaI and cloning the FLAG-tagged GABPβ sequence into pTRE-tight (Clontech, Mountain View, CA, USA). .. To create the NRF-1 expression vector, pTRE-tight-GABPβ was digested with BglII and MluI to remove the GABPβ sequence, but retain the FLAG sequence.

Article Title: Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoelii Infection
Article Snippet: Six smaller overlapping regions of the MAEBL-3 region (which contained different segments of the M2 region, the interdomain between M2 and the repeats, or the repeats) were designed and then subcloned into the pDisplay vector (Invitrogen) together with MAEBL-4 and MAEBL-5 constructs. .. The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent).

Purification:

Article Title: Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoelii Infection
Article Snippet: The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent). .. Bacterial clones were screened for positive transformants by colony PCR with T7 and BgH primers.

Transgenic Assay:

Article Title: Male Sterility of an AHAS-Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L.
Article Snippet: Then, BnAHAS1544T from pMD19T-BnAHAS1544T was introduced into the modified pCAMBIA3301 by digesting with XmaI and BstEII (TaKaRa) and ligating with T4 DNA ligase. .. The constructed vector CaMV35S:: BnAHAS1544T was confirmed by sequencing, and then transformed into A. thaliana (Col-0) by Agrobacterium tumefaciens -mediated floral-dip method ( ).

Generated:

Article Title: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex
Article Snippet: The p3×FLAG-CMV-10 vector was prepared by first cutting with BamHI, followed by a Klenow fill-in reaction, and then digestion using XbaI. .. The final FLAG-GABPβ construct was generated by the ligation of these two fragments. pTRE-tight-GABPβ was prepared by digesting FLAG-GABPβ with SacI (partial) and XmaI and cloning the FLAG-tagged GABPβ sequence into pTRE-tight (Clontech, Mountain View, CA, USA). .. To create the NRF-1 expression vector, pTRE-tight-GABPβ was digested with BglII and MluI to remove the GABPβ sequence, but retain the FLAG sequence.

Polymerase Chain Reaction:

Article Title: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex
Article Snippet: The GABPβ PCR product was then cloned into the pMAL-c2 vector (New England Biolabs (NEB), Pickering, Canada) using the restriction enzymes XbaI/SalI. .. The final FLAG-GABPβ construct was generated by the ligation of these two fragments. pTRE-tight-GABPβ was prepared by digesting FLAG-GABPβ with SacI (partial) and XmaI and cloning the FLAG-tagged GABPβ sequence into pTRE-tight (Clontech, Mountain View, CA, USA).

Article Title: Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoelii Infection
Article Snippet: Genes of interest were amplified with Phusion Hot Start II polymerase (Finnzymes) according to the manufacturer's instructions by using the Bio-Rad Peltier thermal cycler. .. The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent). .. Bacterial clones were screened for positive transformants by colony PCR with T7 and BgH primers.

cDNA Library Assay:

Article Title: Potentiation of Amyotrophic Lateral Sclerosis (ALS)-associated TDP-43 Aggregation by the Proteasome-targeting Factor, Ubiquilin 1
Article Snippet: Our findings suggest that UBQLN is a polyubiquitin-TDP-43 cochaperone that mediates the autophagosomal delivery and/or proteasome targeting of TDP-43 aggregates. .. Yeast Two-hybrid Screening and DNA Construction —We screened a human fetal brain cDNA library (Matchmaker™GAL4 Two-Hybrid System3, Clontech) using a full-length TDP-43 cDNA cloned into XmaI and BamHI sites of the bait vector pGBKT7 (Clontech). .. Approximately 7.5 × 106 cDNA clones, which represents over 2-fold coverage of the library, were screened using standard procedures recommended by the Clontech user manual and Saccharomyces cerevisiae strain AH109 as a cotransformation host.

Expressing:

Article Title: Male Sterility of an AHAS-Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L.
Article Snippet: To determine whether the allele BnAHAS1544T in K5 was responsible for TBM- resistance or not, we constructed its expression vector CaMV35S:: BnAHAS1544T using pCAMBIA3301 as a backbone. .. Then, BnAHAS1544T from pMD19T-BnAHAS1544T was introduced into the modified pCAMBIA3301 by digesting with XmaI and BstEII (TaKaRa) and ligating with T4 DNA ligase.

Article Title: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex
Article Snippet: The final FLAG-GABPβ construct was generated by the ligation of these two fragments. pTRE-tight-GABPβ was prepared by digesting FLAG-GABPβ with SacI (partial) and XmaI and cloning the FLAG-tagged GABPβ sequence into pTRE-tight (Clontech, Mountain View, CA, USA). .. The PCR product was digested with BglII and MluI and cloned into pTRE-tight with the FLAG sequence to create pTRE-tight-NRF-1.

Article Title: Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoelii Infection
Article Snippet: The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent). .. The plasmids were purified with the Nucleobond AX Endotoxin-Free Midiprep kit (Macherey-Nagel) before transfection.

Sequencing:

Article Title: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex
Article Snippet: The p3×FLAG-CMV-10 vector was prepared by first cutting with BamHI, followed by a Klenow fill-in reaction, and then digestion using XbaI. .. The final FLAG-GABPβ construct was generated by the ligation of these two fragments. pTRE-tight-GABPβ was prepared by digesting FLAG-GABPβ with SacI (partial) and XmaI and cloning the FLAG-tagged GABPβ sequence into pTRE-tight (Clontech, Mountain View, CA, USA). .. To create the NRF-1 expression vector, pTRE-tight-GABPβ was digested with BglII and MluI to remove the GABPβ sequence, but retain the FLAG sequence.

Transformation Assay:

Article Title: Male Sterility of an AHAS-Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L.
Article Snippet: Paragraph title: Arabidopsis Transformation and TBM-Treatment ... Then, BnAHAS1544T from pMD19T-BnAHAS1544T was introduced into the modified pCAMBIA3301 by digesting with XmaI and BstEII (TaKaRa) and ligating with T4 DNA ligase.

Article Title: Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoelii Infection
Article Snippet: Genes of interest were amplified with Phusion Hot Start II polymerase (Finnzymes) according to the manufacturer's instructions by using the Bio-Rad Peltier thermal cycler. .. The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent). .. Bacterial clones were screened for positive transformants by colony PCR with T7 and BgH primers.

Recombinant:

Article Title: Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoelii Infection
Article Snippet: The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent). .. The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent).

Plasmid Preparation:

Article Title: Male Sterility of an AHAS-Mutant Induced by Tribenuron-Methyl Solution Correlated With the Decrease of AHAS Activity in Brassica napus L.
Article Snippet: To determine whether the allele BnAHAS1544T in K5 was responsible for TBM- resistance or not, we constructed its expression vector CaMV35S:: BnAHAS1544T using pCAMBIA3301 as a backbone. .. Then, BnAHAS1544T from pMD19T-BnAHAS1544T was introduced into the modified pCAMBIA3301 by digesting with XmaI and BstEII (TaKaRa) and ligating with T4 DNA ligase.

Article Title: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex
Article Snippet: The p3×FLAG-CMV-10 vector was prepared by first cutting with BamHI, followed by a Klenow fill-in reaction, and then digestion using XbaI. .. The final FLAG-GABPβ construct was generated by the ligation of these two fragments. pTRE-tight-GABPβ was prepared by digesting FLAG-GABPβ with SacI (partial) and XmaI and cloning the FLAG-tagged GABPβ sequence into pTRE-tight (Clontech, Mountain View, CA, USA).

Article Title: Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoelii Infection
Article Snippet: Genes of interest were amplified with Phusion Hot Start II polymerase (Finnzymes) according to the manufacturer's instructions by using the Bio-Rad Peltier thermal cycler. .. The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent). .. Bacterial clones were screened for positive transformants by colony PCR with T7 and BgH primers.

Article Title: Potentiation of Amyotrophic Lateral Sclerosis (ALS)-associated TDP-43 Aggregation by the Proteasome-targeting Factor, Ubiquilin 1
Article Snippet: Our findings suggest that UBQLN is a polyubiquitin-TDP-43 cochaperone that mediates the autophagosomal delivery and/or proteasome targeting of TDP-43 aggregates. .. Yeast Two-hybrid Screening and DNA Construction —We screened a human fetal brain cDNA library (Matchmaker™GAL4 Two-Hybrid System3, Clontech) using a full-length TDP-43 cDNA cloned into XmaI and BamHI sites of the bait vector pGBKT7 (Clontech). .. Approximately 7.5 × 106 cDNA clones, which represents over 2-fold coverage of the library, were screened using standard procedures recommended by the Clontech user manual and Saccharomyces cerevisiae strain AH109 as a cotransformation host.

Software:

Article Title: Immunization with the MAEBL M2 Domain Protects against Lethal Plasmodium yoelii Infection
Article Snippet: Primers were designed to be in frame, complementary, and specific to different regions of MAEBL without self-annealing sequences with the software Amplify3x. .. The PCR products were ligated into the XmaI- and SacII (NEB)-linearized pDisplay vector according to the manufacturer's recommendations (In-Fusion Cloning; Clontech) before heat shock transformation into XL10-Gold bacteria (Agilent).

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  • 92
    TaKaRa xmai sites
    Xmai Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xmai sites/product/TaKaRa
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
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    92/100 stars
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    TaKaRa xmai saci cleaved pact2
    Xmai Saci Cleaved Pact2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xmai saci cleaved pact2/product/TaKaRa
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xmai saci cleaved pact2 - by Bioz Stars, 2019-12
    79/100 stars
      Buy from Supplier

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