Structured Review

Promega xmai
Xmai, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 7 article reviews
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xmai - by Bioz Stars, 2019-12
94/100 stars

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Related Articles

Clone Assay:

Article Title: Identification and characterization of DNA sequences that prevent glucocorticoid receptor binding to nearby response elements
Article Snippet: These two data sets (bound and unbound) were then scanned for exact matches of the word TTAATTAA within 10bp bins (RSAT dna-pattern ). .. Reporter plasmids to test the effect of candidate sequences on nearby GBSs (‘Negative Regulatory Sequences (NRS) reporters’) were constructed by first inserting the GBS sequence of interest (see Supplementary Table S1) by ligating oligonucleotides with overhangs to facilitate direct cloning into the XmaI and BglII sites of pGL3 promoter (Promega). .. Subsequently, oligonucleotides encoding the candidate or control sequence with overhangs to facilitate direct cloning (see Supplementary Table S1) were ligated into the Asp718 and NotI sites.

Article Title: v-Myb Mediates Cooperation of a Cell-Specific Enhancer with the mim-1 Promoter
Article Snippet: Some reporter genes contained the mim-1 promoter instead of the tk promoter. .. First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega). .. The resulting plasmid (pGL3-240-Luc) was then used to insert the mim-1 enhancer fragment upstream of the promoter in both orientations, resulting in plasmids pGL3-mim3mim-Luc (1 to 795) and pGL3-mim4mim-Luc (795 to 1).

Amplification:

Article Title: Identification and characterization of DNA sequences that prevent glucocorticoid receptor binding to nearby response elements
Article Snippet: Reporter plasmids to test the effect of candidate sequences on nearby GBSs (‘Negative Regulatory Sequences (NRS) reporters’) were constructed by first inserting the GBS sequence of interest (see Supplementary Table S1) by ligating oligonucleotides with overhangs to facilitate direct cloning into the XmaI and BglII sites of pGL3 promoter (Promega). .. Constructs to stably integrate the NRS reporters at the AAVS1 locus were designed as described previously ( ).

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: In order to delete the nar genes, we amplified a 0.68 kb region upstream of narL (PVE_r1g2542) and a 0.8 kb region downstream of narI (PVE_r1g2549) using primers that introduce XmaI (5' ttttttttttCCCGGGatgctcacccatccccac 3') and EcoRI (5' ttttttttttGAATTCtcaaccggcttgatacccc 3'), or XbaI (5' ttttttttttTCTAGAggcacgttgaggttgtagatg 3'), and XmaI (5' ttttttttttCCCGGGgcaaagcatgctcagcc 3') sites, respectively. .. Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI.

Stable Transfection:

Article Title: Identification and characterization of DNA sequences that prevent glucocorticoid receptor binding to nearby response elements
Article Snippet: Reporter plasmids to test the effect of candidate sequences on nearby GBSs (‘Negative Regulatory Sequences (NRS) reporters’) were constructed by first inserting the GBS sequence of interest (see Supplementary Table S1) by ligating oligonucleotides with overhangs to facilitate direct cloning into the XmaI and BglII sites of pGL3 promoter (Promega). .. Constructs with NRS2 or control sequence shifted by 10 bp or 20 bp were cloned using the oligos listed in Supplementary Table S1 to facilitate direct cloning into the BglII and Asp718 sites of PGL3 promoter.

Construct:

Article Title: Identification and characterization of DNA sequences that prevent glucocorticoid receptor binding to nearby response elements
Article Snippet: These two data sets (bound and unbound) were then scanned for exact matches of the word TTAATTAA within 10bp bins (RSAT dna-pattern ). .. Reporter plasmids to test the effect of candidate sequences on nearby GBSs (‘Negative Regulatory Sequences (NRS) reporters’) were constructed by first inserting the GBS sequence of interest (see Supplementary Table S1) by ligating oligonucleotides with overhangs to facilitate direct cloning into the XmaI and BglII sites of pGL3 promoter (Promega). .. Subsequently, oligonucleotides encoding the candidate or control sequence with overhangs to facilitate direct cloning (see Supplementary Table S1) were ligated into the Asp718 and NotI sites.

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI. .. A plasmid with the correct inserts was purified and retransformed into E . coli S17-1 lambda-pir, from which it was transferred into P . veronii 1YdBTEX2 by conjugation as described elsewhere [ ].

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase. .. The 14 kb Dmp1 regulatory sequence, containing a 9.6 kb fragment of the Dmp1 promoter region together with exon 1, intron 1 and the noncoding region of exon 2, was released from pSK vector by KpNI and XmaI (vector kindly provided by Dr. Jerry Feng, Texas A & M University Baylor College of Dentistry).

Article Title: Calcium Sensing Receptor Mutations Implicated in Pancreatitis and Idiopathic Epilepsy Syndrome Disrupt an Arginine-rich Retention Motif
Article Snippet: Constructs were generated in the background of human CaSR in pEGFP-N1 with an amino terminal FLAG epitope [ ] using Pfu ultra polymerase (Stratagene). .. Full length CaSR and duplexes were digested with XmaI and BamHI (Promega) for 3 hours at 37o C, run on 1% agarose gels and purified with the Qiagen QiaEXII kit.

Article Title: v-Myb Mediates Cooperation of a Cell-Specific Enhancer with the mim-1 Promoter
Article Snippet: First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega). .. First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega).

Article Title: Tissue-specific Expression of the L1 Cell Adhesion Molecule Is Modulated by the Neural Restrictive Silencer Element
Article Snippet: RNA start sites were located using an RNAse protection assay (Ambion, Inc., Austin, TX). .. L1-1 through L1-6 were constructed by insertion of L1 genomic fragments into the XmaI and XhoI sites of pGL2basic ( Promega Corp. , Madison, WI). .. To prepare L1-5N and L1-5Nr plasmids, double-stranded oligonucleotides containing two copies of the NRSE from the L1 gene were inserted into the XmaI site upstream of the L1-5 promoter fragment.

Luciferase:

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: The AcGFP-mem cDNA was retrieved from the pAcGFP1-Mem plasmid by XmaI and NotI restriction endonucleases, and was blunted at the NotI end. .. It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase. .. The resultant construct was designated pGL-AcGFP1-Mem.

Article Title: Tissue-specific Expression of the L1 Cell Adhesion Molecule Is Modulated by the Neural Restrictive Silencer Element
Article Snippet: Paragraph title: Preparation of Luciferase and lacZ Reporter Constructs ... L1-1 through L1-6 were constructed by insertion of L1 genomic fragments into the XmaI and XhoI sites of pGL2basic ( Promega Corp. , Madison, WI).

Expressing:

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI. .. P . veronii recombinants were selected on MM agar plates containing 50 μg ml–1 kanamycin with toluene vapor as carbon source, and verified by PCR for the appropriate integration of the construct.

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: Paragraph title: Generation of transgenic mice expressing GFP selectively in osteocytes ... It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase.

Modification:

Article Title: Tissue-specific Expression of the L1 Cell Adhesion Molecule Is Modulated by the Neural Restrictive Silencer Element
Article Snippet: L1-1 through L1-6 were constructed by insertion of L1 genomic fragments into the XmaI and XhoI sites of pGL2basic ( Promega Corp. , Madison, WI). .. L1-1 through L1-6 were constructed by insertion of L1 genomic fragments into the XmaI and XhoI sites of pGL2basic ( Promega Corp. , Madison, WI).

Transformation Assay:

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: PCR products were size-verified by agarose gel electrophoresis, purified using a Nucleospin Gel and PCR cleanup kit (Macherey-Nagel AG, Oensingen, Switzerland), ligated into pGEM-T-Easy (Promega AG, Dübendorf, Switzerland) and transformed into Escherichia coli DH5alpha. .. Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI.

Conjugation Assay:

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI. .. Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI.

Transfection:

Article Title: v-Myb Mediates Cooperation of a Cell-Specific Enhancer with the mim-1 Promoter
Article Snippet: Paragraph title: Reporter genes and transfections. ... First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega).

Sequencing:

Article Title: Identification and characterization of DNA sequences that prevent glucocorticoid receptor binding to nearby response elements
Article Snippet: These two data sets (bound and unbound) were then scanned for exact matches of the word TTAATTAA within 10bp bins (RSAT dna-pattern ). .. Reporter plasmids to test the effect of candidate sequences on nearby GBSs (‘Negative Regulatory Sequences (NRS) reporters’) were constructed by first inserting the GBS sequence of interest (see Supplementary Table S1) by ligating oligonucleotides with overhangs to facilitate direct cloning into the XmaI and BglII sites of pGL3 promoter (Promega). .. Subsequently, oligonucleotides encoding the candidate or control sequence with overhangs to facilitate direct cloning (see Supplementary Table S1) were ligated into the Asp718 and NotI sites.

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase. .. The resultant construct was designated pGL-AcGFP1-Mem.

Article Title: v-Myb Mediates Cooperation of a Cell-Specific Enhancer with the mim-1 Promoter
Article Snippet: First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega). .. First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega).

Introduce:

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: In order to delete the nar genes, we amplified a 0.68 kb region upstream of narL (PVE_r1g2542) and a 0.8 kb region downstream of narI (PVE_r1g2549) using primers that introduce XmaI (5' ttttttttttCCCGGGatgctcacccatccccac 3') and EcoRI (5' ttttttttttGAATTCtcaaccggcttgatacccc 3'), or XbaI (5' ttttttttttTCTAGAggcacgttgaggttgtagatg 3'), and XmaI (5' ttttttttttCCCGGGgcaaagcatgctcagcc 3') sites, respectively. .. Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI.

Generated:

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: However, in order to more clearly image living and fixed osteocytes and better resolve their dendrites in situ within bone, we have generated a new transgenic mouse line expressing a membrane targeted GFP variant (AcGFP1-mem) in osteocytes using the 9.6 kb fragment of the dentin matrix protein-1 (Dmp1) promoter to drive expression. .. It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase.

Article Title: Calcium Sensing Receptor Mutations Implicated in Pancreatitis and Idiopathic Epilepsy Syndrome Disrupt an Arginine-rich Retention Motif
Article Snippet: CaSR(3A) (CaSR(R896A/K897A/R898A)), and CaSR(5A) (CaSR(R890A/R891A/R896A/K897A/R898A)) were generated in the full length CaSR and the CaSRΔ898 truncation using seventy-five base pair complementary oligonucleotides with the appropriate mutations, an XmaI restriction site at the 5’ end and a BamHI restriction site at the 3’ end. .. Full length CaSR and duplexes were digested with XmaI and BamHI (Promega) for 3 hours at 37o C, run on 1% agarose gels and purified with the Qiagen QiaEXII kit.

Article Title: v-Myb Mediates Cooperation of a Cell-Specific Enhancer with the mim-1 Promoter
Article Snippet: First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega). .. First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega).

Imaging:

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: These mice express a GFP that is localized within the cytoplasm and this mouse line has been proven very useful for imaging/lineage tracing of osteocytes in vitro and in vivo [ , ] as well as for the generation of immortalized cell lines that recapitulate osteocyte differentiation [ ]. .. It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase.

Polymerase Chain Reaction:

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: PCR products were size-verified by agarose gel electrophoresis, purified using a Nucleospin Gel and PCR cleanup kit (Macherey-Nagel AG, Oensingen, Switzerland), ligated into pGEM-T-Easy (Promega AG, Dübendorf, Switzerland) and transformed into Escherichia coli DH5alpha. .. Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI.

Article Title: Calcium Sensing Receptor Mutations Implicated in Pancreatitis and Idiopathic Epilepsy Syndrome Disrupt an Arginine-rich Retention Motif
Article Snippet: Truncations in CaSR were generated by inserting a stop codon by PCR mutagenesis. .. Full length CaSR and duplexes were digested with XmaI and BamHI (Promega) for 3 hours at 37o C, run on 1% agarose gels and purified with the Qiagen QiaEXII kit.

Article Title: v-Myb Mediates Cooperation of a Cell-Specific Enhancer with the mim-1 Promoter
Article Snippet: First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega). .. First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega).

Binding Assay:

Article Title: v-Myb Mediates Cooperation of a Cell-Specific Enhancer with the mim-1 Promoter
Article Snippet: The Myb binding sites were mutated as follows. .. First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega).

In Vivo:

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: These mice express a GFP that is localized within the cytoplasm and this mouse line has been proven very useful for imaging/lineage tracing of osteocytes in vitro and in vivo [ , ] as well as for the generation of immortalized cell lines that recapitulate osteocyte differentiation [ ]. .. It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase.

Electroporation:

Article Title: v-Myb Mediates Cooperation of a Cell-Specific Enhancer with the mim-1 Promoter
Article Snippet: First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega). .. DNA transfection of adherent cells (HD11, 10.4, and QT6) was performed by calcium-phosphate coprecipitation as described previously ( ).

Mutagenesis:

Article Title: Calcium Sensing Receptor Mutations Implicated in Pancreatitis and Idiopathic Epilepsy Syndrome Disrupt an Arginine-rich Retention Motif
Article Snippet: Similar approaches were used to generate the R890A/R891A mutant in CaSRΔ898. .. Full length CaSR and duplexes were digested with XmaI and BamHI (Promega) for 3 hours at 37o C, run on 1% agarose gels and purified with the Qiagen QiaEXII kit.

Article Title: v-Myb Mediates Cooperation of a Cell-Specific Enhancer with the mim-1 Promoter
Article Snippet: First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega). .. First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega).

Mouse Assay:

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: Paragraph title: Generation of transgenic mice expressing GFP selectively in osteocytes ... It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase.

Transgenic Assay:

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: Paragraph title: Generation of transgenic mice expressing GFP selectively in osteocytes ... It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase.

Purification:

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: PCR products were size-verified by agarose gel electrophoresis, purified using a Nucleospin Gel and PCR cleanup kit (Macherey-Nagel AG, Oensingen, Switzerland), ligated into pGEM-T-Easy (Promega AG, Dübendorf, Switzerland) and transformed into Escherichia coli DH5alpha. .. Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI. .. E . coli DH5alpha-lambda-pir transformants were recovered on nutrient agar plates with 50 μg ml–1 of kanamycin.

Article Title: Calcium Sensing Receptor Mutations Implicated in Pancreatitis and Idiopathic Epilepsy Syndrome Disrupt an Arginine-rich Retention Motif
Article Snippet: CaSR(3A) (CaSR(R896A/K897A/R898A)), and CaSR(5A) (CaSR(R890A/R891A/R896A/K897A/R898A)) were generated in the full length CaSR and the CaSRΔ898 truncation using seventy-five base pair complementary oligonucleotides with the appropriate mutations, an XmaI restriction site at the 5’ end and a BamHI restriction site at the 3’ end. .. Full length CaSR and duplexes were digested with XmaI and BamHI (Promega) for 3 hours at 37o C, run on 1% agarose gels and purified with the Qiagen QiaEXII kit. .. Digested and purified CaSR was then dephosphorylated with shrimp alkaline phosphatase (Promega M820A) according to the manufacturer's protocol, and ligated with T4 DNA Ligase (Promega M1801).

Plasmid Preparation:

Article Title: Identification and characterization of DNA sequences that prevent glucocorticoid receptor binding to nearby response elements
Article Snippet: Reporter plasmids to test the effect of candidate sequences on nearby GBSs (‘Negative Regulatory Sequences (NRS) reporters’) were constructed by first inserting the GBS sequence of interest (see Supplementary Table S1) by ligating oligonucleotides with overhangs to facilitate direct cloning into the XmaI and BglII sites of pGL3 promoter (Promega). .. Constructs to stably integrate the NRS reporters at the AAVS1 locus were designed as described previously ( ).

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: PCR products were size-verified by agarose gel electrophoresis, purified using a Nucleospin Gel and PCR cleanup kit (Macherey-Nagel AG, Oensingen, Switzerland), ligated into pGEM-T-Easy (Promega AG, Dübendorf, Switzerland) and transformed into Escherichia coli DH5alpha. .. Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI. .. E . coli DH5alpha-lambda-pir transformants were recovered on nutrient agar plates with 50 μg ml–1 of kanamycin.

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: The AcGFP-mem cDNA was retrieved from the pAcGFP1-Mem plasmid by XmaI and NotI restriction endonucleases, and was blunted at the NotI end. .. It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase. .. The resultant construct was designated pGL-AcGFP1-Mem.

Article Title: v-Myb Mediates Cooperation of a Cell-Specific Enhancer with the mim-1 Promoter
Article Snippet: First, the mim-1 promoter region (−240 to +150 bp) was cloned as a XmaI/BglII fragment between the XmaI and BglII sites of pGL3-Basic (Promega). .. The resulting plasmid (pGL3-240-Luc) was then used to insert the mim-1 enhancer fragment upstream of the promoter in both orientations, resulting in plasmids pGL3-mim3mim-Luc (1 to 795) and pGL3-mim4mim-Luc (795 to 1).

Article Title: Tissue-specific Expression of the L1 Cell Adhesion Molecule Is Modulated by the Neural Restrictive Silencer Element
Article Snippet: L1-1 through L1-6 were constructed by insertion of L1 genomic fragments into the XmaI and XhoI sites of pGL2basic ( Promega Corp. , Madison, WI). .. L1-1 through L1-6 were constructed by insertion of L1 genomic fragments into the XmaI and XhoI sites of pGL2basic ( Promega Corp. , Madison, WI).

In Situ:

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: However, in order to more clearly image living and fixed osteocytes and better resolve their dendrites in situ within bone, we have generated a new transgenic mouse line expressing a membrane targeted GFP variant (AcGFP1-mem) in osteocytes using the 9.6 kb fragment of the dentin matrix protein-1 (Dmp1) promoter to drive expression. .. It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase.

Negative Control:

Article Title: Tissue-specific Expression of the L1 Cell Adhesion Molecule Is Modulated by the Neural Restrictive Silencer Element
Article Snippet: L1-1 through L1-6 were constructed by insertion of L1 genomic fragments into the XmaI and XhoI sites of pGL2basic ( Promega Corp. , Madison, WI). .. L1-7 through L1-12, L1lacZ, and L1lacZΔN were prepared using the vector CMVβ (CLONTECH Laboratories, Inc.), after replacing the human cytomegalovirus promoter with a polylinker containing the XmaI and XhoI sites, which allowed the insertion of the L1 genomic fragments. lacZ constructs were converted into luciferase reporters by removing the lacZ gene by digestion with NotI and replacing it with a modified luciferase gene cassette from pGEMluc ( Promega Corp. ) containing an SV-40 polyadenylation signal.

Agarose Gel Electrophoresis:

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: PCR products were size-verified by agarose gel electrophoresis, purified using a Nucleospin Gel and PCR cleanup kit (Macherey-Nagel AG, Oensingen, Switzerland), ligated into pGEM-T-Easy (Promega AG, Dübendorf, Switzerland) and transformed into Escherichia coli DH5alpha. .. Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI.

In Vitro:

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: These mice express a GFP that is localized within the cytoplasm and this mouse line has been proven very useful for imaging/lineage tracing of osteocytes in vitro and in vivo [ , ] as well as for the generation of immortalized cell lines that recapitulate osteocyte differentiation [ ]. .. It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase.

FLAG-tag:

Article Title: Calcium Sensing Receptor Mutations Implicated in Pancreatitis and Idiopathic Epilepsy Syndrome Disrupt an Arginine-rich Retention Motif
Article Snippet: Constructs were generated in the background of human CaSR in pEGFP-N1 with an amino terminal FLAG epitope [ ] using Pfu ultra polymerase (Stratagene). .. Full length CaSR and duplexes were digested with XmaI and BamHI (Promega) for 3 hours at 37o C, run on 1% agarose gels and purified with the Qiagen QiaEXII kit.

Respiration Assay:

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: Paragraph title: Gene marker exchange and nitrate respiration assay ... Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI.

Marker:

Article Title: The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand
Article Snippet: Paragraph title: Gene marker exchange and nitrate respiration assay ... Plasmids were purified using a Nucleospin Plasmid kit (Macherey-Nagel) and sequenced for verification, after which inserts were recovered by digestion with XbaI and XmaI (Promega), or with XmaI and EcoRI, and ligated with plasmid pJP5603 I-SceI v2 [ ], pre-digested with XbaI and EcoRI.

Variant Assay:

Article Title: Novel approaches for two and three dimensional multiplexed imaging of osteocytes
Article Snippet: However, in order to more clearly image living and fixed osteocytes and better resolve their dendrites in situ within bone, we have generated a new transgenic mouse line expressing a membrane targeted GFP variant (AcGFP1-mem) in osteocytes using the 9.6 kb fragment of the dentin matrix protein-1 (Dmp1) promoter to drive expression. .. It was then subcloned into the XmaI and blunted XbaI sites of the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the cDNA encoding firefly luciferase.

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    Promega xmai
    Xmai, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xmai/product/Promega
    Average 94 stars, based on 8 article reviews
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    xmai - by Bioz Stars, 2019-12
    94/100 stars
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    82
    Promega pgl3 vector xbai xmai
    Pgl3 Vector Xbai Xmai, supplied by Promega, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgl3 vector xbai xmai/product/Promega
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pgl3 vector xbai xmai - by Bioz Stars, 2019-12
    82/100 stars
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