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Promega xmai
Xmai, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xmai/product/Promega
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
xmai - by Bioz Stars, 2021-07
86/100 stars

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Related Articles

Electrophoresis:

Article Title: Escherichia coliehl1 Gene-Positive Serotype O18ac:H31 Associated with an Outbreak of Diarrhea in a Neonatal Nursery in Neuqu?n City, Argentina
Article Snippet: The plugs were incubated at 50°C in lysis buffer overnight and sliced into aliquots 2 mm in thickness. .. The DNA-containing plugs were subjected to restriction endonuclease digestion with 20 U of Xba I (Promega Corp., Madison, Wis.) at 37°C for 18 h. Restriction fragments were separated by electrophoresis through 1% PFGE agarose (Bio-Rad) in 0.5× Tris-borate-EDTA buffer at 14°C at 200 V in a CHEFF DR III apparatus (Bio-Rad). .. A linearly ramped switching time from 5 to 40 s was applied for 20 h. The gels were stained with ethidium bromide and photographed in a UV transilluminator.

other:

Article Title: Characterization of a Recurrent Clonal Type of Escherichia coli O157:H7 Causing Major Outbreaks of Infection in Scotland
Article Snippet: Cleavage of the agarose-embedded DNA was achieved with Xba I (Promega, Southampton, United Kingdom), Not I (Promega), Sfi I (Promega), Xho I (Boehringer Mannheim, Lewes, United Kingdom), and Avr II (Boehringer Mannheim) according to the manufacturer's instructions.

Incubation:

Article Title: Enhancing the catalytic repertoire of nucleic acids. II. Simultaneous incorporation of amino and imidazolyl functionalities by two modified triphosphates during PCR
Article Snippet: PCR products formed using modified C7-dATP derivatives and control reactions were ethanol precipitated and digested according to the manufacturer’s protocols. .. The DNA was incubated with Xba I (Promega) in 6 mM Tris–HCl pH 7.9, 150 mM NaCl, 1 mM DTT for 1.5 h at 37°C for DNA containing natural nucleotides and for 1.5 h at 37°C and then overnight at room temperature for DNA containing modified nucleotides. .. Alternatively the DNA was incubated with Sma I (Promega) in 10 mM Tris–HCl pH 7.0, 7 mM MgCl2 , 50 mM KCl, 1 mM DTT for 1.5 h at 25°C for DNA containing natural nucleotides and for 1.5 h at 25°C and then overnight at room temperature for DNA containing modified nucleotides.

Modification:

Article Title: Enhancing the catalytic repertoire of nucleic acids. II. Simultaneous incorporation of amino and imidazolyl functionalities by two modified triphosphates during PCR
Article Snippet: PCR products formed using modified C7-dATP derivatives and control reactions were ethanol precipitated and digested according to the manufacturer’s protocols. .. The DNA was incubated with Xba I (Promega) in 6 mM Tris–HCl pH 7.9, 150 mM NaCl, 1 mM DTT for 1.5 h at 37°C for DNA containing natural nucleotides and for 1.5 h at 37°C and then overnight at room temperature for DNA containing modified nucleotides. .. Alternatively the DNA was incubated with Sma I (Promega) in 10 mM Tris–HCl pH 7.0, 7 mM MgCl2 , 50 mM KCl, 1 mM DTT for 1.5 h at 25°C for DNA containing natural nucleotides and for 1.5 h at 25°C and then overnight at room temperature for DNA containing modified nucleotides.

Reporter Assay:

Article Title: Exosome miR-371b-5p promotes proliferation of lung alveolar progenitor type II cells by using PTEN to orchestrate the PI3K/Akt signaling
Article Snippet: In addition, 10 μg ATIIC phenotype-derived exosomal proteins were analyzed for exosome marker expression by using rabbit anti-TSG101, mouse anti-CD63 and mouse anti-HSP70 antibody (Abcam). .. Dual-luciferase miRNA target reporter assay To generate the dual-luciferase miRNA target reporter vector, a double-stranded oligonucleotide containing wild-type (wt) PTEN 3′UTR sequence with a predicted miR-371b-5p target site or mutant PTEN 3′UTR sequence was cloned into Pmel and XbaI sites in pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega, Fig. ). .. The hiPSC-ATIICs of 70% confluence in 24-well plates were co-transfected with the dual-luciferase miRNA target reporter vector (0.3 μg) and miR-371b-5p mimic (15 pmol/well).

Plasmid Preparation:

Article Title: Exosome miR-371b-5p promotes proliferation of lung alveolar progenitor type II cells by using PTEN to orchestrate the PI3K/Akt signaling
Article Snippet: In addition, 10 μg ATIIC phenotype-derived exosomal proteins were analyzed for exosome marker expression by using rabbit anti-TSG101, mouse anti-CD63 and mouse anti-HSP70 antibody (Abcam). .. Dual-luciferase miRNA target reporter assay To generate the dual-luciferase miRNA target reporter vector, a double-stranded oligonucleotide containing wild-type (wt) PTEN 3′UTR sequence with a predicted miR-371b-5p target site or mutant PTEN 3′UTR sequence was cloned into Pmel and XbaI sites in pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega, Fig. ). .. The hiPSC-ATIICs of 70% confluence in 24-well plates were co-transfected with the dual-luciferase miRNA target reporter vector (0.3 μg) and miR-371b-5p mimic (15 pmol/well).

Article Title: Deregulation of Poly(A) Polymerase Interferes with Cell Growth
Article Snippet: Probe 1 in the RNase protection assays was prepared from chicken PAP III DNA: a fragment of 268 nt that contains 191 nt from exon 12 and 77 nt from intron 12 ( ) was amplified by PCR and subcloned into a pBluescript vector; the vector was linearized by Bss HII and in vitro transcribed with T7 RNA polymerase. .. Probe 2 was prepared from chicken PAP II cDNA: the last 400 bp in the open reading frame of chicken PAP II cDNA was amplified by PCR and subcloned into a pBluescript vector; the plasmid was linearized with Xba I and in vitro transcribed with T7 RNA polymerase (Promega). ..

Sequencing:

Article Title: Exosome miR-371b-5p promotes proliferation of lung alveolar progenitor type II cells by using PTEN to orchestrate the PI3K/Akt signaling
Article Snippet: In addition, 10 μg ATIIC phenotype-derived exosomal proteins were analyzed for exosome marker expression by using rabbit anti-TSG101, mouse anti-CD63 and mouse anti-HSP70 antibody (Abcam). .. Dual-luciferase miRNA target reporter assay To generate the dual-luciferase miRNA target reporter vector, a double-stranded oligonucleotide containing wild-type (wt) PTEN 3′UTR sequence with a predicted miR-371b-5p target site or mutant PTEN 3′UTR sequence was cloned into Pmel and XbaI sites in pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega, Fig. ). .. The hiPSC-ATIICs of 70% confluence in 24-well plates were co-transfected with the dual-luciferase miRNA target reporter vector (0.3 μg) and miR-371b-5p mimic (15 pmol/well).

Mutagenesis:

Article Title: Exosome miR-371b-5p promotes proliferation of lung alveolar progenitor type II cells by using PTEN to orchestrate the PI3K/Akt signaling
Article Snippet: In addition, 10 μg ATIIC phenotype-derived exosomal proteins were analyzed for exosome marker expression by using rabbit anti-TSG101, mouse anti-CD63 and mouse anti-HSP70 antibody (Abcam). .. Dual-luciferase miRNA target reporter assay To generate the dual-luciferase miRNA target reporter vector, a double-stranded oligonucleotide containing wild-type (wt) PTEN 3′UTR sequence with a predicted miR-371b-5p target site or mutant PTEN 3′UTR sequence was cloned into Pmel and XbaI sites in pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega, Fig. ). .. The hiPSC-ATIICs of 70% confluence in 24-well plates were co-transfected with the dual-luciferase miRNA target reporter vector (0.3 μg) and miR-371b-5p mimic (15 pmol/well).

Clone Assay:

Article Title: Exosome miR-371b-5p promotes proliferation of lung alveolar progenitor type II cells by using PTEN to orchestrate the PI3K/Akt signaling
Article Snippet: In addition, 10 μg ATIIC phenotype-derived exosomal proteins were analyzed for exosome marker expression by using rabbit anti-TSG101, mouse anti-CD63 and mouse anti-HSP70 antibody (Abcam). .. Dual-luciferase miRNA target reporter assay To generate the dual-luciferase miRNA target reporter vector, a double-stranded oligonucleotide containing wild-type (wt) PTEN 3′UTR sequence with a predicted miR-371b-5p target site or mutant PTEN 3′UTR sequence was cloned into Pmel and XbaI sites in pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega, Fig. ). .. The hiPSC-ATIICs of 70% confluence in 24-well plates were co-transfected with the dual-luciferase miRNA target reporter vector (0.3 μg) and miR-371b-5p mimic (15 pmol/well).

Expressing:

Article Title: Exosome miR-371b-5p promotes proliferation of lung alveolar progenitor type II cells by using PTEN to orchestrate the PI3K/Akt signaling
Article Snippet: In addition, 10 μg ATIIC phenotype-derived exosomal proteins were analyzed for exosome marker expression by using rabbit anti-TSG101, mouse anti-CD63 and mouse anti-HSP70 antibody (Abcam). .. Dual-luciferase miRNA target reporter assay To generate the dual-luciferase miRNA target reporter vector, a double-stranded oligonucleotide containing wild-type (wt) PTEN 3′UTR sequence with a predicted miR-371b-5p target site or mutant PTEN 3′UTR sequence was cloned into Pmel and XbaI sites in pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega, Fig. ). .. The hiPSC-ATIICs of 70% confluence in 24-well plates were co-transfected with the dual-luciferase miRNA target reporter vector (0.3 μg) and miR-371b-5p mimic (15 pmol/well).

Amplification:

Article Title: Deregulation of Poly(A) Polymerase Interferes with Cell Growth
Article Snippet: Probe 1 in the RNase protection assays was prepared from chicken PAP III DNA: a fragment of 268 nt that contains 191 nt from exon 12 and 77 nt from intron 12 ( ) was amplified by PCR and subcloned into a pBluescript vector; the vector was linearized by Bss HII and in vitro transcribed with T7 RNA polymerase. .. Probe 2 was prepared from chicken PAP II cDNA: the last 400 bp in the open reading frame of chicken PAP II cDNA was amplified by PCR and subcloned into a pBluescript vector; the plasmid was linearized with Xba I and in vitro transcribed with T7 RNA polymerase (Promega). ..

Polymerase Chain Reaction:

Article Title: Deregulation of Poly(A) Polymerase Interferes with Cell Growth
Article Snippet: Probe 1 in the RNase protection assays was prepared from chicken PAP III DNA: a fragment of 268 nt that contains 191 nt from exon 12 and 77 nt from intron 12 ( ) was amplified by PCR and subcloned into a pBluescript vector; the vector was linearized by Bss HII and in vitro transcribed with T7 RNA polymerase. .. Probe 2 was prepared from chicken PAP II cDNA: the last 400 bp in the open reading frame of chicken PAP II cDNA was amplified by PCR and subcloned into a pBluescript vector; the plasmid was linearized with Xba I and in vitro transcribed with T7 RNA polymerase (Promega). ..

In Vitro:

Article Title: Deregulation of Poly(A) Polymerase Interferes with Cell Growth
Article Snippet: Probe 1 in the RNase protection assays was prepared from chicken PAP III DNA: a fragment of 268 nt that contains 191 nt from exon 12 and 77 nt from intron 12 ( ) was amplified by PCR and subcloned into a pBluescript vector; the vector was linearized by Bss HII and in vitro transcribed with T7 RNA polymerase. .. Probe 2 was prepared from chicken PAP II cDNA: the last 400 bp in the open reading frame of chicken PAP II cDNA was amplified by PCR and subcloned into a pBluescript vector; the plasmid was linearized with Xba I and in vitro transcribed with T7 RNA polymerase (Promega). ..

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