Structured Review

Millipore xmai
Xmai, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xmai/product/Millipore
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
xmai - by Bioz Stars, 2020-07
90/100 stars

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Related Articles

Clone Assay:

Article Title: RAB-Like 2 Has an Essential Role in Male Fertility, Sperm Intra-Flagellar Transport, and Tail Assembly
Article Snippet: .. Recombinant RABL2 production Full-length Rabl2 (isoform 2) was amplified from wild type testes cDNA using the primers: 5′-ATAACCCGGGGTTCTGCAGGGGACAGAAACAGGCA-3′ and 5′-ATCTCCATGGTTAGGAAGGAGATGGGCTCTTG-3′ , then cloned into the XmaI and Ncol sites of pET32a (Novagen). .. Recombinant RABL2 was produced and the histidine tag removed as described previously .

Article Title: Structures of two bacterial resistance factors mediating tRNA-dependent aminoacylation of phosphatidylglycerol with lysine or alanine
Article Snippet: .. Base pairs 1627–2643 of ORF PA0920 from P . aeruginosa PAO1 and base pairs 1555–2550 of ORF yfiX from B . licheniformis DSM13 were PCR-amplified using oligonucleotide pairs 1 and 2 and 3 and 4 , respectively, and were cloned into the XmaI and SacI sites of pET52b(+) (Novagen) for expression with a cleavable N-terminal Strep -II-tag. .. The SerP (surface entropy reduction) server ( ) was used to identify mutations that may facilitate optimized crystallization of A-PGS (KGKE674 to AGAA674 ).

Article Title: Arabidopsis protein disulfide isomerase-8 is a type I endoplasmic reticulum transmembrane protein with thiol-disulfide oxidase activity
Article Snippet: .. Alkaline phosphatase activity assay A construct for the heterologous expression of PDI8 in E. coli was generated by cloning the coding sequence for the lumenal portion of PDI8, containing the catalytic a domain and redox-inactive b and b’ domains (PDI8abb’ , Fig. ) between the XmaI and SalI restriction sites of the bacterial expression vector pFLAG-CTS (Sigma-Aldrich, St. Louis, MO). .. The PDI8 abb’ gene fragment was amplified from a full-length PDI8 cDNA using a forward primer with an XmaI site (5’-TGT CCC GGG AGA TGA TCA ATT CAC CCT CGA C-3’) and a reverse primer with a SalI site (5’-AAT GTC GAC CAT TGA GTT GAT AAA TCC CAT G-3’).

Amplification:

Article Title: RAB-Like 2 Has an Essential Role in Male Fertility, Sperm Intra-Flagellar Transport, and Tail Assembly
Article Snippet: .. Recombinant RABL2 production Full-length Rabl2 (isoform 2) was amplified from wild type testes cDNA using the primers: 5′-ATAACCCGGGGTTCTGCAGGGGACAGAAACAGGCA-3′ and 5′-ATCTCCATGGTTAGGAAGGAGATGGGCTCTTG-3′ , then cloned into the XmaI and Ncol sites of pET32a (Novagen). .. Recombinant RABL2 was produced and the histidine tag removed as described previously .

Polymerase Chain Reaction:

Article Title: Critical Contribution of Tyr15 in the HIV-1 Integrase (IN) in Facilitating IN Assembly and Nonenzymatic Function through the IN Precursor Form with Reverse Transcriptase
Article Snippet: .. The resulting PCR fragments were digested with XmaI and XhoI and ligated into a pET47b(+) vector (Novagen) using its XmaI and XhoI sites. pNL-Nh is a mutant of the NL4-3 proviral clone ( ) in which an NheI restriction enzyme cleavage site was blunted and religated, introducing frameshift mutations in the env gene ( ). .. S15-dTomato is a plasmid expressing the 15 N-terminal membrane-targeting amino acids of p60c-Src fused to red fluorescent protein dTomato ( ).

Article Title: Structures of two bacterial resistance factors mediating tRNA-dependent aminoacylation of phosphatidylglycerol with lysine or alanine
Article Snippet: .. Base pairs 1627–2643 of ORF PA0920 from P . aeruginosa PAO1 and base pairs 1555–2550 of ORF yfiX from B . licheniformis DSM13 were PCR-amplified using oligonucleotide pairs 1 and 2 and 3 and 4 , respectively, and were cloned into the XmaI and SacI sites of pET52b(+) (Novagen) for expression with a cleavable N-terminal Strep -II-tag. .. The SerP (surface entropy reduction) server ( ) was used to identify mutations that may facilitate optimized crystallization of A-PGS (KGKE674 to AGAA674 ).

Mutagenesis:

Article Title: Critical Contribution of Tyr15 in the HIV-1 Integrase (IN) in Facilitating IN Assembly and Nonenzymatic Function through the IN Precursor Form with Reverse Transcriptase
Article Snippet: .. The resulting PCR fragments were digested with XmaI and XhoI and ligated into a pET47b(+) vector (Novagen) using its XmaI and XhoI sites. pNL-Nh is a mutant of the NL4-3 proviral clone ( ) in which an NheI restriction enzyme cleavage site was blunted and religated, introducing frameshift mutations in the env gene ( ). .. S15-dTomato is a plasmid expressing the 15 N-terminal membrane-targeting amino acids of p60c-Src fused to red fluorescent protein dTomato ( ).

Construct:

Article Title: Arabidopsis protein disulfide isomerase-8 is a type I endoplasmic reticulum transmembrane protein with thiol-disulfide oxidase activity
Article Snippet: .. Alkaline phosphatase activity assay A construct for the heterologous expression of PDI8 in E. coli was generated by cloning the coding sequence for the lumenal portion of PDI8, containing the catalytic a domain and redox-inactive b and b’ domains (PDI8abb’ , Fig. ) between the XmaI and SalI restriction sites of the bacterial expression vector pFLAG-CTS (Sigma-Aldrich, St. Louis, MO). .. The PDI8 abb’ gene fragment was amplified from a full-length PDI8 cDNA using a forward primer with an XmaI site (5’-TGT CCC GGG AGA TGA TCA ATT CAC CCT CGA C-3’) and a reverse primer with a SalI site (5’-AAT GTC GAC CAT TGA GTT GAT AAA TCC CAT G-3’).

Article Title: Rotavirus Replication Requires a Functional Proteasome for Effective Assembly of Viroplasms ▿
Article Snippet: .. In order to construct the plasmid pVAX-T7-segment11-ribo-T7stop, indicated in the Results section as pVAX-segment 11, the pVAX vector (Invitrogen) was modified by deletion of the cytomegalovirus (CMV) promoter using NdeI and ApaI restriction enzymes and subsequent insertion of an oligonucleotide containing the XmaI, ApaI, and SacII restriction sites (5′-TATGGGTACCTACTCCCGGGTTACAGGGCCCGCGGAGGCC-3′; Sigma). .. Between the sites of XmaI and ApaI, a synthetic sequence (Genscript) has been inserted in order to clone the ribozyme and the T7 terminator (5′- ccCGGG TCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGTCGTCCACTCGGATGGCTAAGGGAGAGCTcggatccGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATCGAGATCCtctaga gggccc -3′; capital letters indicate the ribozyme, and the T7 terminator and the XmaI and ApaI sites are underlined).

Sequencing:

Article Title: Arabidopsis protein disulfide isomerase-8 is a type I endoplasmic reticulum transmembrane protein with thiol-disulfide oxidase activity
Article Snippet: .. Alkaline phosphatase activity assay A construct for the heterologous expression of PDI8 in E. coli was generated by cloning the coding sequence for the lumenal portion of PDI8, containing the catalytic a domain and redox-inactive b and b’ domains (PDI8abb’ , Fig. ) between the XmaI and SalI restriction sites of the bacterial expression vector pFLAG-CTS (Sigma-Aldrich, St. Louis, MO). .. The PDI8 abb’ gene fragment was amplified from a full-length PDI8 cDNA using a forward primer with an XmaI site (5’-TGT CCC GGG AGA TGA TCA ATT CAC CCT CGA C-3’) and a reverse primer with a SalI site (5’-AAT GTC GAC CAT TGA GTT GAT AAA TCC CAT G-3’).

Generated:

Article Title: Arabidopsis protein disulfide isomerase-8 is a type I endoplasmic reticulum transmembrane protein with thiol-disulfide oxidase activity
Article Snippet: .. Alkaline phosphatase activity assay A construct for the heterologous expression of PDI8 in E. coli was generated by cloning the coding sequence for the lumenal portion of PDI8, containing the catalytic a domain and redox-inactive b and b’ domains (PDI8abb’ , Fig. ) between the XmaI and SalI restriction sites of the bacterial expression vector pFLAG-CTS (Sigma-Aldrich, St. Louis, MO). .. The PDI8 abb’ gene fragment was amplified from a full-length PDI8 cDNA using a forward primer with an XmaI site (5’-TGT CCC GGG AGA TGA TCA ATT CAC CCT CGA C-3’) and a reverse primer with a SalI site (5’-AAT GTC GAC CAT TGA GTT GAT AAA TCC CAT G-3’).

Expressing:

Article Title: Structures of two bacterial resistance factors mediating tRNA-dependent aminoacylation of phosphatidylglycerol with lysine or alanine
Article Snippet: .. Base pairs 1627–2643 of ORF PA0920 from P . aeruginosa PAO1 and base pairs 1555–2550 of ORF yfiX from B . licheniformis DSM13 were PCR-amplified using oligonucleotide pairs 1 and 2 and 3 and 4 , respectively, and were cloned into the XmaI and SacI sites of pET52b(+) (Novagen) for expression with a cleavable N-terminal Strep -II-tag. .. The SerP (surface entropy reduction) server ( ) was used to identify mutations that may facilitate optimized crystallization of A-PGS (KGKE674 to AGAA674 ).

Article Title: Arabidopsis protein disulfide isomerase-8 is a type I endoplasmic reticulum transmembrane protein with thiol-disulfide oxidase activity
Article Snippet: .. Alkaline phosphatase activity assay A construct for the heterologous expression of PDI8 in E. coli was generated by cloning the coding sequence for the lumenal portion of PDI8, containing the catalytic a domain and redox-inactive b and b’ domains (PDI8abb’ , Fig. ) between the XmaI and SalI restriction sites of the bacterial expression vector pFLAG-CTS (Sigma-Aldrich, St. Louis, MO). .. The PDI8 abb’ gene fragment was amplified from a full-length PDI8 cDNA using a forward primer with an XmaI site (5’-TGT CCC GGG AGA TGA TCA ATT CAC CCT CGA C-3’) and a reverse primer with a SalI site (5’-AAT GTC GAC CAT TGA GTT GAT AAA TCC CAT G-3’).

ALP Activity Assay:

Article Title: Arabidopsis protein disulfide isomerase-8 is a type I endoplasmic reticulum transmembrane protein with thiol-disulfide oxidase activity
Article Snippet: .. Alkaline phosphatase activity assay A construct for the heterologous expression of PDI8 in E. coli was generated by cloning the coding sequence for the lumenal portion of PDI8, containing the catalytic a domain and redox-inactive b and b’ domains (PDI8abb’ , Fig. ) between the XmaI and SalI restriction sites of the bacterial expression vector pFLAG-CTS (Sigma-Aldrich, St. Louis, MO). .. The PDI8 abb’ gene fragment was amplified from a full-length PDI8 cDNA using a forward primer with an XmaI site (5’-TGT CCC GGG AGA TGA TCA ATT CAC CCT CGA C-3’) and a reverse primer with a SalI site (5’-AAT GTC GAC CAT TGA GTT GAT AAA TCC CAT G-3’).

Modification:

Article Title: Rotavirus Replication Requires a Functional Proteasome for Effective Assembly of Viroplasms ▿
Article Snippet: .. In order to construct the plasmid pVAX-T7-segment11-ribo-T7stop, indicated in the Results section as pVAX-segment 11, the pVAX vector (Invitrogen) was modified by deletion of the cytomegalovirus (CMV) promoter using NdeI and ApaI restriction enzymes and subsequent insertion of an oligonucleotide containing the XmaI, ApaI, and SacII restriction sites (5′-TATGGGTACCTACTCCCGGGTTACAGGGCCCGCGGAGGCC-3′; Sigma). .. Between the sites of XmaI and ApaI, a synthetic sequence (Genscript) has been inserted in order to clone the ribozyme and the T7 terminator (5′- ccCGGG TCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGTCGTCCACTCGGATGGCTAAGGGAGAGCTcggatccGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATCGAGATCCtctaga gggccc -3′; capital letters indicate the ribozyme, and the T7 terminator and the XmaI and ApaI sites are underlined).

Recombinant:

Article Title: RAB-Like 2 Has an Essential Role in Male Fertility, Sperm Intra-Flagellar Transport, and Tail Assembly
Article Snippet: .. Recombinant RABL2 production Full-length Rabl2 (isoform 2) was amplified from wild type testes cDNA using the primers: 5′-ATAACCCGGGGTTCTGCAGGGGACAGAAACAGGCA-3′ and 5′-ATCTCCATGGTTAGGAAGGAGATGGGCTCTTG-3′ , then cloned into the XmaI and Ncol sites of pET32a (Novagen). .. Recombinant RABL2 was produced and the histidine tag removed as described previously .

Plasmid Preparation:

Article Title: LARP4 mRNA codon-tRNA match contributes to LARP4 activity for ribosomal protein mRNA poly(A) tail length protection
Article Snippet: .. After restriction digestion with KpnI, HindIII and XmaI, the CDS and 3’UTR were ligated and inserted into HindIII and XmaI sites of pFlag-CMV2 vector (Sigma-Aldrich). .. Constructs containing 3 copies per plasmid of each tRNA gene for TyrGUA, PheGAA, ThrUGU or ProAGG, in pUC57-Kan were obtained from Genewiz.

Article Title: Critical Contribution of Tyr15 in the HIV-1 Integrase (IN) in Facilitating IN Assembly and Nonenzymatic Function through the IN Precursor Form with Reverse Transcriptase
Article Snippet: .. The resulting PCR fragments were digested with XmaI and XhoI and ligated into a pET47b(+) vector (Novagen) using its XmaI and XhoI sites. pNL-Nh is a mutant of the NL4-3 proviral clone ( ) in which an NheI restriction enzyme cleavage site was blunted and religated, introducing frameshift mutations in the env gene ( ). .. S15-dTomato is a plasmid expressing the 15 N-terminal membrane-targeting amino acids of p60c-Src fused to red fluorescent protein dTomato ( ).

Article Title: Arabidopsis protein disulfide isomerase-8 is a type I endoplasmic reticulum transmembrane protein with thiol-disulfide oxidase activity
Article Snippet: .. Alkaline phosphatase activity assay A construct for the heterologous expression of PDI8 in E. coli was generated by cloning the coding sequence for the lumenal portion of PDI8, containing the catalytic a domain and redox-inactive b and b’ domains (PDI8abb’ , Fig. ) between the XmaI and SalI restriction sites of the bacterial expression vector pFLAG-CTS (Sigma-Aldrich, St. Louis, MO). .. The PDI8 abb’ gene fragment was amplified from a full-length PDI8 cDNA using a forward primer with an XmaI site (5’-TGT CCC GGG AGA TGA TCA ATT CAC CCT CGA C-3’) and a reverse primer with a SalI site (5’-AAT GTC GAC CAT TGA GTT GAT AAA TCC CAT G-3’).

Article Title: Rotavirus Replication Requires a Functional Proteasome for Effective Assembly of Viroplasms ▿
Article Snippet: .. In order to construct the plasmid pVAX-T7-segment11-ribo-T7stop, indicated in the Results section as pVAX-segment 11, the pVAX vector (Invitrogen) was modified by deletion of the cytomegalovirus (CMV) promoter using NdeI and ApaI restriction enzymes and subsequent insertion of an oligonucleotide containing the XmaI, ApaI, and SacII restriction sites (5′-TATGGGTACCTACTCCCGGGTTACAGGGCCCGCGGAGGCC-3′; Sigma). .. Between the sites of XmaI and ApaI, a synthetic sequence (Genscript) has been inserted in order to clone the ribozyme and the T7 terminator (5′- ccCGGG TCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGTCGTCCACTCGGATGGCTAAGGGAGAGCTcggatccGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATCGAGATCCtctaga gggccc -3′; capital letters indicate the ribozyme, and the T7 terminator and the XmaI and ApaI sites are underlined).

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