Structured Review

Stratagene xl 10
Xl 10, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xl 10/product/Stratagene
Average 88 stars, based on 4 article reviews
Price from $9.99 to $1999.99
xl 10 - by Bioz Stars, 2020-08
88/100 stars

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Mutagenesis:

Article Title: Characterization of the Heme Environment in Arabidopsis thaliana Fatty Acid α-Dioxygenase-1
Article Snippet: .. QuikChange site-directed mutagenesis kit and Escherichia coli strains DH5α, XL-10, and BL21(DE3)pLysS were from Stratagene (La Jolla, CA). .. Restriction enzymes and DNA-modifying enzymes were from New England Biolabs (Beverly, MA).

Clone Assay:

Article Title: IdeR is required for iron homeostasis and virulence in Mycobacterium tuberculosis
Article Snippet: .. Escherichia coli strains JM109 and XL-10 (Stratagene) used for cloning were grown in Luria-Bertani (LB) broth. .. Mtb strains were maintained in 7H10 agar or 7H9 broth (Difco) supplemented with 0.2% glycerol, 0.05% Tween 80, 0.5% bovine serum albumin (BSA), 0.2% dextrose and 0.085% NaCl (ADN).

Article Title: A Ferritin Mutant of Mycobacterium tuberculosis Is Highly Susceptible to Killing by Antibiotics and Is Unable To Establish a Chronic Infection in Mice
Article Snippet: .. Escherichia coli strains JM109 and XL-10 (Stratagene) were used for cloning and were grown in Luria-Bertani (LB) broth. .. M. tuberculosis strains were maintained in 7H10 agar (Difco) supplemented with 0.2% glycerol, 0.05% Tween 80, and 10% ADN supplement (0.5% albumin, 0.2% dextrose, 0.085% NaCl).

Article Title: The mycobacterial iron dependent regulator IdeR induces ferritin (bfrB) by alleviating Lsr2 repression
Article Snippet: .. Escherichia coli strains GM161 or XL-10 (Stratagene) were used for cloning and BL21(DE3) rosetta strain (Novagen) was used for protein overexpression. ..

Article Title: MntR(Rv2788) a transcriptional regulator that controls manganese homeostasis in Mycobacterium tuberculosis
Article Snippet: .. Escherichia coli strains JM109, XL-10 (stratagene) and HB101 used for cloning were grown in Luria-Bertani (LB) media. .. M. tuberculosis H37Rv and derived strains were maintained in 7H10 agar or 7H9 broth (Difco) supplemented with 0.2% glycerol, 0.05% Tween 80 and ADN (0.5% BSA, 0.2% dextrose and 0.085% NaCl).

Recombinant:

Article Title: Genetics of Critical Contacts and Clashes in the DNA Packaging Specificities of Bacteriophages λ and 21
Article Snippet: .. Competent cells for recombinant DNA manipulations were XL-10 (Stratagene) and DH5α Library Efficiency (Invitrogen). .. Plasmid pJB0 is pBR322 ( ) carrying the λ DNA segment that extends from the Hind III site at λ bp 44141, across cos and the terminase genes, to the BamH I site at 5505.

Over Expression:

Article Title: The mycobacterial iron dependent regulator IdeR induces ferritin (bfrB) by alleviating Lsr2 repression
Article Snippet: .. Escherichia coli strains GM161 or XL-10 (Stratagene) were used for cloning and BL21(DE3) rosetta strain (Novagen) was used for protein overexpression. ..

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  • 89
    Stratagene e coli strain xl 1 blue
    Selection of Phabs binding to TouRΔA. The number of phage bound to ELISA plates coated with 6xhis TouRΔA after each panning round is indicated. The bound phage were eluted from the plates by incubation with 0.1 M glycine (pH 2.5) as explained in Materials and Methods. After recovery, the titers of these phage were determined on E . coli <t>XL-1</t> Blue cells and selected for AMP resistance. In each panning round, the number of input phage was kept constant at 2 × 10 11 PFU and the phage that did not bind 6xhis TouRΔA were removed by 40 washing steps with PBS. The increase in the number of bound phage after the second round of panning is indicative of a preferential amplification of Phab clones binding to 6xhis TouRΔA.
    E Coli Strain Xl 1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strain xl 1 blue/product/Stratagene
    Average 89 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    e coli strain xl 1 blue - by Bioz Stars, 2020-08
    89/100 stars
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    86
    Stratagene xl 10 gold ultra competent e coli
    Selection of Phabs binding to TouRΔA. The number of phage bound to ELISA plates coated with 6xhis TouRΔA after each panning round is indicated. The bound phage were eluted from the plates by incubation with 0.1 M glycine (pH 2.5) as explained in Materials and Methods. After recovery, the titers of these phage were determined on E . coli <t>XL-1</t> Blue cells and selected for AMP resistance. In each panning round, the number of input phage was kept constant at 2 × 10 11 PFU and the phage that did not bind 6xhis TouRΔA were removed by 40 washing steps with PBS. The increase in the number of bound phage after the second round of panning is indicative of a preferential amplification of Phab clones binding to 6xhis TouRΔA.
    Xl 10 Gold Ultra Competent E Coli, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xl 10 gold ultra competent e coli/product/Stratagene
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xl 10 gold ultra competent e coli - by Bioz Stars, 2020-08
    86/100 stars
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    85
    Stratagene recombination deficient competent xl 10 gold bacteria
    Selection of Phabs binding to TouRΔA. The number of phage bound to ELISA plates coated with 6xhis TouRΔA after each panning round is indicated. The bound phage were eluted from the plates by incubation with 0.1 M glycine (pH 2.5) as explained in Materials and Methods. After recovery, the titers of these phage were determined on E . coli <t>XL-1</t> Blue cells and selected for AMP resistance. In each panning round, the number of input phage was kept constant at 2 × 10 11 PFU and the phage that did not bind 6xhis TouRΔA were removed by 40 washing steps with PBS. The increase in the number of bound phage after the second round of panning is indicative of a preferential amplification of Phab clones binding to 6xhis TouRΔA.
    Recombination Deficient Competent Xl 10 Gold Bacteria, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombination deficient competent xl 10 gold bacteria/product/Stratagene
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    recombination deficient competent xl 10 gold bacteria - by Bioz Stars, 2020-08
    85/100 stars
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    88
    Stratagene escherichia coli xl 10 gold
    Selection of Phabs binding to TouRΔA. The number of phage bound to ELISA plates coated with 6xhis TouRΔA after each panning round is indicated. The bound phage were eluted from the plates by incubation with 0.1 M glycine (pH 2.5) as explained in Materials and Methods. After recovery, the titers of these phage were determined on E . coli <t>XL-1</t> Blue cells and selected for AMP resistance. In each panning round, the number of input phage was kept constant at 2 × 10 11 PFU and the phage that did not bind 6xhis TouRΔA were removed by 40 washing steps with PBS. The increase in the number of bound phage after the second round of panning is indicative of a preferential amplification of Phab clones binding to 6xhis TouRΔA.
    Escherichia Coli Xl 10 Gold, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli xl 10 gold/product/Stratagene
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    escherichia coli xl 10 gold - by Bioz Stars, 2020-08
    88/100 stars
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    Selection of Phabs binding to TouRΔA. The number of phage bound to ELISA plates coated with 6xhis TouRΔA after each panning round is indicated. The bound phage were eluted from the plates by incubation with 0.1 M glycine (pH 2.5) as explained in Materials and Methods. After recovery, the titers of these phage were determined on E . coli XL-1 Blue cells and selected for AMP resistance. In each panning round, the number of input phage was kept constant at 2 × 10 11 PFU and the phage that did not bind 6xhis TouRΔA were removed by 40 washing steps with PBS. The increase in the number of bound phage after the second round of panning is indicative of a preferential amplification of Phab clones binding to 6xhis TouRΔA.

    Journal: Journal of Bacteriology

    Article Title: Monitoring Intracellular Levels of XylR in Pseudomonas putida with a Single-Chain Antibody Specific for Aromatic-Responsive Enhancer-Binding Proteins

    doi: 10.1128/JB.183.19.5571-5579.2001

    Figure Lengend Snippet: Selection of Phabs binding to TouRΔA. The number of phage bound to ELISA plates coated with 6xhis TouRΔA after each panning round is indicated. The bound phage were eluted from the plates by incubation with 0.1 M glycine (pH 2.5) as explained in Materials and Methods. After recovery, the titers of these phage were determined on E . coli XL-1 Blue cells and selected for AMP resistance. In each panning round, the number of input phage was kept constant at 2 × 10 11 PFU and the phage that did not bind 6xhis TouRΔA were removed by 40 washing steps with PBS. The increase in the number of bound phage after the second round of panning is indicative of a preferential amplification of Phab clones binding to 6xhis TouRΔA.

    Article Snippet: The E. coli strain XL-1 Blue ( recA1 gyrA96 relA1 endA1 hsdR17 supE44 thi1 lac [F′ proAB lacI q lacZ ΔM15 Tn 10 ] Tcr ; Stratagene) was used as host for bacteriophages and phagemids.

    Techniques: Selection, Binding Assay, Enzyme-linked Immunosorbent Assay, Incubation, Amplification, Clone Assay

    Amino acid sequence of scFv B7. The amino acid sequence of the scFv B7 polypeptide encoded by the phagemid is shown. The positions of the N-terminal signal peptide, the V H domain, the (Gly 4 Ser) 3 linker peptide, the V L domain, and the E tag are indicated. The complementarity-determining regions (CDR) of the V H and V L domains are labeled and underlined. The site of cleavage of the bacterial signal peptidase is marked by an arrow. The five amino acid changes found in the scFv B9 are marked below the sequence of scFv B7. When produced in E . coli XL-1 Blue cells ( supE ), these scFvs are also synthesized as hybrids with protein 3 of M13. The location of the suppressed stop codon (amber), which is placed between the scFv and protein 3 coding sequences, is indicated.

    Journal: Journal of Bacteriology

    Article Title: Monitoring Intracellular Levels of XylR in Pseudomonas putida with a Single-Chain Antibody Specific for Aromatic-Responsive Enhancer-Binding Proteins

    doi: 10.1128/JB.183.19.5571-5579.2001

    Figure Lengend Snippet: Amino acid sequence of scFv B7. The amino acid sequence of the scFv B7 polypeptide encoded by the phagemid is shown. The positions of the N-terminal signal peptide, the V H domain, the (Gly 4 Ser) 3 linker peptide, the V L domain, and the E tag are indicated. The complementarity-determining regions (CDR) of the V H and V L domains are labeled and underlined. The site of cleavage of the bacterial signal peptidase is marked by an arrow. The five amino acid changes found in the scFv B9 are marked below the sequence of scFv B7. When produced in E . coli XL-1 Blue cells ( supE ), these scFvs are also synthesized as hybrids with protein 3 of M13. The location of the suppressed stop codon (amber), which is placed between the scFv and protein 3 coding sequences, is indicated.

    Article Snippet: The E. coli strain XL-1 Blue ( recA1 gyrA96 relA1 endA1 hsdR17 supE44 thi1 lac [F′ proAB lacI q lacZ ΔM15 Tn 10 ] Tcr ; Stratagene) was used as host for bacteriophages and phagemids.

    Techniques: Sequencing, Labeling, Produced, Synthesized