Structured Review

GE Healthcare empty chromatography columns xk 26 40
Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.
Empty Chromatography Columns Xk 26 40, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/empty chromatography columns xk 26 40/product/GE Healthcare
Average 79 stars, based on 59 article reviews
Price from $9.99 to $1999.99
empty chromatography columns xk 26 40 - by Bioz Stars, 2020-01
79/100 stars

Images

1) Product Images from "Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography"

Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

doi: 10.1016/j.jchromb.2008.02.014

Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.
Figure Legend Snippet: Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

Techniques Used: Purification, Injection, Generated

Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.
Figure Legend Snippet: Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

Techniques Used: Purification, Injection, Generated

2) Product Images from "Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography"

Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

doi: 10.1016/j.jchromb.2008.02.014

Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.
Figure Legend Snippet: Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

Techniques Used: Purification, Injection, Generated

Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.
Figure Legend Snippet: Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

Techniques Used: Purification, Injection, Generated

Related Articles

Centrifugation:

Article Title: Structures of closed and open conformations of dimeric human ATM
Article Snippet: Cells were harvested 16 hours later by centrifugation and lysed using a high-pressure homogenizer (Avestin, Biopharma) in a lysis buffer containing 50 mM Hepes (pH 7.4, 4°C), 500 mM NaCl, 5% (v/v) glycerol, 20 mM imidazole, 0.05% (v/v) Tween 20, 8 mM β-mercaptoethanol, EDTA-free protease inhibitor cocktail (Roche), ribonuclease A (Sigma-Aldrich), deoxyribonuclease I (Sigma-Aldrich), and lysozyme (Sigma-Aldrich). .. Finally, the elution fractions were directly subjected to SEC using an XK 26/70 Superose 6 prep grade column (GE Healthcare) equilibrated in 20 mM Hepes (pH 7.3, 4°C), 500 mM NaCl, 200 mM arginine, 10% (v/v) glycerol, and 4 mM DTT.

Article Title: Polyglycine hydrolases: Fungal β-lactamase-like endoproteases that cleave polyglycine regions within plant class IV chitinases
Article Snippet: Particulate material was separated from the protein extract by a combination of filtration and centrifugation. .. The clarified extract was injected onto a Capto MMC XK 26/20 column (GE Healthcare, Waukesha WI) resulting in capture of Es-cmp.

Article Title: The relationship between folding and activity in UreG, an intrinsically disordered enzyme
Article Snippet: The cell pellet was separated from the supernatant by centrifugation at 14,000 rpm for 30 min at 4 °C. .. The supertnatant of the lysis was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been pre-equilibrated with 2 volumes of 20 mM Tris-HCl buffer pH 8, containing 1 mM DTT and 5 mM EDTA.

Article Title: Expression, purification and crystallization of pecan (Carya illinoinensis) vicilin
Article Snippet: The sample was subjected to centrifugation at 20 000 g for 30 min at 20°C again and the supernatant was loaded onto a 5 ml HisTrap FF Crude column (GE Healthcare, Piscataway, New Jersey, USA). .. The main peak of the HisTrap elution was loaded onto a XK 26/70 Superdex-200 column (GE Healthcare) pre-equilibrated and eluted with 10 m M Tris–HCl pH 7.9, 500 m M NaCl at a flow rate of 1 ml min−1 .

Article Title: Familial secondary erythrocytosis due to increased oxygen affinity is caused by destabilization of the T state of hemoglobin Brigham (?2Pro100Leu)
Article Snippet: HbA was purified following osmotic lysis and centrifugation within 1 day of collection according to established methods. .. Catalase was removed from wild-type Hb using an XK 26/100 column containing Superdex-200 chromatography media (GE Healthcare Biosciences, Piscataway, NJ).

Article Title: Structure and Protein–Protein Interactions of Methanol Dehydrogenase from Methylococcus capsulatus (Bath)
Article Snippet: The cell debris was removed by centrifugation at 24000g for 1 h, and membranes were separated from the soluble proteins by ultracentrifugation at 160000g for an additional 1 h. The membranes were then washed to isolate pMMO as described previously, and the soluble fraction was used to purify MDH. .. The supernatant was loaded onto a DEAE-Sepharose FF XK 26/20 column (GE Healthcare) and washed with 20 mM Tris (pH 8.0) until all unbound protein eluted.

Article Title: Unraveling the Helicobacter pylori UreG zinc binding site using X-ray absorption spectroscopy (XAS) and structural modeling
Article Snippet: Cell debris was separated from the supernatant by centrifugation at 15,000g for 30 min at 4 °C. .. The soluble fraction obtained from the cell lysate was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been preequilibrated with 20 mM Tris–HCl buffer, pH 7.0, containing 2 mM EDTA.

Filtration:

Article Title: Dataset on protein composition of a human plasma sub-proteome able to modulate the Dengue 2 virus infection in Huh 7.5 cells
Article Snippet: Briefly, 125 mL of pooled human plasma were dialyzed against equilibration buffer (50 mM Hepes pH 6, 60 mM NaCl, 1 mM EDTA) and then centrifuged at 10,000 g for 30 min at 4 °C, diluted 3-fold in equilibration buffer and subjected to tandem filtration through Whatman 1 qualitative filter paper (Whatman, UK) and a 0.45 µm membrane. .. The resulting sample was loaded at a flow rate of 10 cm/h onto an XK-26 chromatography column (GE Healthcare, USA) packed with 10 mL of DE-52 gel, which was next washed with equilibration buffer until absorbance at 280 nm returned to baseline (bound protein represented, in average, 1.2% of the initial protein contents of the sample and no more than 60% of the total protein binding capacity estimated for DE-52 at pH 6.0).

Article Title: Polyglycine hydrolases: Fungal β-lactamase-like endoproteases that cleave polyglycine regions within plant class IV chitinases
Article Snippet: Particulate material was separated from the protein extract by a combination of filtration and centrifugation. .. The clarified extract was injected onto a Capto MMC XK 26/20 column (GE Healthcare, Waukesha WI) resulting in capture of Es-cmp.

Article Title: Novel Mechanism of Hemin Capture by Hbp2, the Hemoglobin-binding Hemophore from Listeria monocytogenes *
Article Snippet: .. After concentration, the protein was then applied onto a Sephacryl S-100 XK 26/100 gel filtration column (GE Healthcare) equilibrated with buffer B, and pure fractions were pooled for crystallography trials. .. The Hbp2N2 -hemin complex was concentrated to 53 mg/ml and crystallized at room temperature using the hanging drop vapor diffusion method in a reservoir solution of 100 m m MES, pH 6.0, 10 m m ZnCl2 , 20% polyethylene glycol 6000.

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: The proteins were eluted with buffer A supplemented with 500 mM imidazole, pH8, after the column was washed with five bed volumes of buffer A. cMCR-1 and cMCR-2 were further purified by size-exclusion chromatography (SEC) using Superdex 200 - XK 26/70 column (GE Healthcare). .. For cMCR-1, the N-terminal His-tag was removed by tobacco etch virus (TEV) protease before gel filtration.

X-ray Diffraction:

Article Title: Novel Mechanism of Hemin Capture by Hbp2, the Hemoglobin-binding Hemophore from Listeria monocytogenes *
Article Snippet: After concentration, the protein was then applied onto a Sephacryl S-100 XK 26/100 gel filtration column (GE Healthcare) equilibrated with buffer B, and pure fractions were pooled for crystallography trials. .. An x-ray diffraction data set was collected on beamline 24-ID-C as described above for apo-Hbp2N2 .

Construct:

Article Title: The relationship between folding and activity in UreG, an intrinsically disordered enzyme
Article Snippet: E. coli BL21(DE3) cells transformed with pET3a-SpureG construct were grown at 37 °C with vigorous stirring. .. The supertnatant of the lysis was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been pre-equilibrated with 2 volumes of 20 mM Tris-HCl buffer pH 8, containing 1 mM DTT and 5 mM EDTA.

Article Title: Familial secondary erythrocytosis due to increased oxygen affinity is caused by destabilization of the T state of hemoglobin Brigham (?2Pro100Leu)
Article Snippet: Catalase was removed from wild-type Hb using an XK 26/100 column containing Superdex-200 chromatography media (GE Healthcare Biosciences, Piscataway, NJ). .. Recombinant hemoglobin Brigham was generated by site-directed mutagenesis of the pHE2 construct that was kindly provided by C. Ho and T.-J.

Incubation:

Article Title: Novel Mechanism of Hemin Capture by Hbp2, the Hemoglobin-binding Hemophore from Listeria monocytogenes *
Article Snippet: The protein/hemin mixture was incubated at room temperature on a tube rotator for 1 h. The excess hemin was removed by separating the mixture using a Sephadex G-25 column. .. After concentration, the protein was then applied onto a Sephacryl S-100 XK 26/100 gel filtration column (GE Healthcare) equilibrated with buffer B, and pure fractions were pooled for crystallography trials.

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: The cell culture was then incubated at 37 °C with shaking until it reached an OD600 of 0.6; at this point, protein production was induced by addition of IPTG at a final concentration of 1 mM. .. The proteins were eluted with buffer A supplemented with 500 mM imidazole, pH8, after the column was washed with five bed volumes of buffer A. cMCR-1 and cMCR-2 were further purified by size-exclusion chromatography (SEC) using Superdex 200 - XK 26/70 column (GE Healthcare).

Diffusion-based Assay:

Article Title: Novel Mechanism of Hemin Capture by Hbp2, the Hemoglobin-binding Hemophore from Listeria monocytogenes *
Article Snippet: After concentration, the protein was then applied onto a Sephacryl S-100 XK 26/100 gel filtration column (GE Healthcare) equilibrated with buffer B, and pure fractions were pooled for crystallography trials. .. The Hbp2N2 -hemin complex was concentrated to 53 mg/ml and crystallized at room temperature using the hanging drop vapor diffusion method in a reservoir solution of 100 m m MES, pH 6.0, 10 m m ZnCl2 , 20% polyethylene glycol 6000.

Expressing:

Article Title: Structures of closed and open conformations of dimeric human ATM
Article Snippet: Paragraph title: Human p53 expression and purification for kinase assay ... Finally, the elution fractions were directly subjected to SEC using an XK 26/70 Superose 6 prep grade column (GE Healthcare) equilibrated in 20 mM Hepes (pH 7.3, 4°C), 500 mM NaCl, 200 mM arginine, 10% (v/v) glycerol, and 4 mM DTT.

Article Title: Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex
Article Snippet: Paragraph title: GyrB and GyrA expression and purification ... The GyrA protein was purified by nickel-affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE Healthcare) bound to Ni2+ ions.

Article Title: The relationship between folding and activity in UreG, an intrinsically disordered enzyme
Article Snippet: When OD600 reached 0.5–0.6, expression was induced by addition of isopropyl β-thiogalactopyranoside (IPTG) to a final concentration of 0.5 mM, and the temperature was decreased to 20 °C. .. The supertnatant of the lysis was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been pre-equilibrated with 2 volumes of 20 mM Tris-HCl buffer pH 8, containing 1 mM DTT and 5 mM EDTA.

Article Title: Unraveling the Helicobacter pylori UreG zinc binding site using X-ray absorption spectroscopy (XAS) and structural modeling
Article Snippet: Paragraph title: Hp UreG expression and purification ... The soluble fraction obtained from the cell lysate was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been preequilibrated with 20 mM Tris–HCl buffer, pH 7.0, containing 2 mM EDTA.

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: Paragraph title: Construction, expression and purification of MCR-1 and MCR-2 recombinant proteins ... The proteins were eluted with buffer A supplemented with 500 mM imidazole, pH8, after the column was washed with five bed volumes of buffer A. cMCR-1 and cMCR-2 were further purified by size-exclusion chromatography (SEC) using Superdex 200 - XK 26/70 column (GE Healthcare).

Article Title: Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who’s Leading HCHT’s Biological Function
Article Snippet: Paragraph title: Hybrid Protein Expression and Purification ... In this study, isolated ChBDCHIT1-49 domains were purified by molecular exclusion chromatography using XK 26/100 SuperDex 75 PrepGrade column (GE Healthcare) equilibrated in 150 mM NaCl 50 mM phosphate buffer pH 7.5 (PBS).

Modification:

Article Title: Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex
Article Snippet: GyrB and GyrA expression and purification The sequence coding for the full-length E . coli GyrA (2-875) was inserted into a modified pET28b containing an N-terminal 10-His tag and a C-terminal Twin-strep tag. .. The GyrA protein was purified by nickel-affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE Healthcare) bound to Ni2+ ions.

Kinase Assay:

Article Title: Structures of closed and open conformations of dimeric human ATM
Article Snippet: Paragraph title: Human p53 expression and purification for kinase assay ... Finally, the elution fractions were directly subjected to SEC using an XK 26/70 Superose 6 prep grade column (GE Healthcare) equilibrated in 20 mM Hepes (pH 7.3, 4°C), 500 mM NaCl, 200 mM arginine, 10% (v/v) glycerol, and 4 mM DTT.

Over Expression:

Article Title: Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex
Article Snippet: Overexpression was performed in E . coli BL21 (DE3) pRARE2 in LB medium containing 50 µg/mL kanamycin and 35 µg/mL chloramphenicol. .. The GyrA protein was purified by nickel-affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE Healthcare) bound to Ni2+ ions.

Crystallization Assay:

Article Title: Novel Mechanism of Hemin Capture by Hbp2, the Hemoglobin-binding Hemophore from Listeria monocytogenes *
Article Snippet: Paragraph title: Crystallization and Structure Determination ... After concentration, the protein was then applied onto a Sephacryl S-100 XK 26/100 gel filtration column (GE Healthcare) equilibrated with buffer B, and pure fractions were pooled for crystallography trials.

Flow Cytometry:

Article Title: Dataset on protein composition of a human plasma sub-proteome able to modulate the Dengue 2 virus infection in Huh 7.5 cells
Article Snippet: .. The resulting sample was loaded at a flow rate of 10 cm/h onto an XK-26 chromatography column (GE Healthcare, USA) packed with 10 mL of DE-52 gel, which was next washed with equilibration buffer until absorbance at 280 nm returned to baseline (bound protein represented, in average, 1.2% of the initial protein contents of the sample and no more than 60% of the total protein binding capacity estimated for DE-52 at pH 6.0). ..

Article Title: Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex
Article Snippet: .. The GyrA protein was purified by nickel-affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE Healthcare) bound to Ni2+ ions. .. Elution was performed with the lysis buffer containing 250 mM imidazol pH 8.0 and eluted proteins were directly attached on a 10 ml Streptavidin Sepharose (GE Healthcare).

Article Title: Induction of Apoptosis and Cytotoxicity by Isothiocyanate Sulforaphene in Human Hepatocarcinoma HepG2 Cells
Article Snippet: The purity was further improved by gel-filtration removal of contaminants, which was performed using an XK 26/100 column packed with Sephadex G10 chromatography media (Amersham BioSciences, Buckinghamshire, UK), connected to an FPLC System (Pharmacia, Kent, UK). .. The mobile phase was water at a flow rate of 2.0 mL min−1 , and the eluate absorbance was monitored at 254 nm.

Article Title: Expression, purification and crystallization of pecan (Carya illinoinensis) vicilin
Article Snippet: .. The main peak of the HisTrap elution was loaded onto a XK 26/70 Superdex-200 column (GE Healthcare) pre-equilibrated and eluted with 10 m M Tris–HCl pH 7.9, 500 m M NaCl at a flow rate of 1 ml min−1 . .. All chromatographic steps were carried out at room temperature using an FPLC system (GE Healthcare).

Chromatography:

Article Title: Dataset on protein composition of a human plasma sub-proteome able to modulate the Dengue 2 virus infection in Huh 7.5 cells
Article Snippet: .. The resulting sample was loaded at a flow rate of 10 cm/h onto an XK-26 chromatography column (GE Healthcare, USA) packed with 10 mL of DE-52 gel, which was next washed with equilibration buffer until absorbance at 280 nm returned to baseline (bound protein represented, in average, 1.2% of the initial protein contents of the sample and no more than 60% of the total protein binding capacity estimated for DE-52 at pH 6.0). ..

Article Title: Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex
Article Snippet: .. The GyrA protein was purified by nickel-affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE Healthcare) bound to Ni2+ ions. .. Elution was performed with the lysis buffer containing 250 mM imidazol pH 8.0 and eluted proteins were directly attached on a 10 ml Streptavidin Sepharose (GE Healthcare).

Article Title: Induction of Apoptosis and Cytotoxicity by Isothiocyanate Sulforaphene in Human Hepatocarcinoma HepG2 Cells
Article Snippet: .. The purity was further improved by gel-filtration removal of contaminants, which was performed using an XK 26/100 column packed with Sephadex G10 chromatography media (Amersham BioSciences, Buckinghamshire, UK), connected to an FPLC System (Pharmacia, Kent, UK). ..

Article Title: Familial secondary erythrocytosis due to increased oxygen affinity is caused by destabilization of the T state of hemoglobin Brigham (?2Pro100Leu)
Article Snippet: .. Catalase was removed from wild-type Hb using an XK 26/100 column containing Superdex-200 chromatography media (GE Healthcare Biosciences, Piscataway, NJ). .. Brigham Hb purification steps are described in greater detail below.

Article Title: Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who’s Leading HCHT’s Biological Function
Article Snippet: .. In this study, isolated ChBDCHIT1-49 domains were purified by molecular exclusion chromatography using XK 26/100 SuperDex 75 PrepGrade column (GE Healthcare) equilibrated in 150 mM NaCl 50 mM phosphate buffer pH 7.5 (PBS). .. Isotopic Labelled Protein Expression and Production for NMR studies 13 C, 15 N or 15 N ChBDCHIT1-49 samples were expressed in E. coli BL21(DE3) cells.

Protease Inhibitor:

Article Title: Dataset on protein composition of a human plasma sub-proteome able to modulate the Dengue 2 virus infection in Huh 7.5 cells
Article Snippet: The resulting sample was loaded at a flow rate of 10 cm/h onto an XK-26 chromatography column (GE Healthcare, USA) packed with 10 mL of DE-52 gel, which was next washed with equilibration buffer until absorbance at 280 nm returned to baseline (bound protein represented, in average, 1.2% of the initial protein contents of the sample and no more than 60% of the total protein binding capacity estimated for DE-52 at pH 6.0). .. Upon collection, a protease inhibitor cocktail consisting of 1 μg/mL leupeptin, 1 μg/mL pepstatin A, 1 μg/mL soybean trypsin inhibitor and 1 mM PMSF was added to the eluted fractions, which were subsequently dialyzed against 10 mM Hepes pH 7.0, 0.15 M NaCl, 1 mM CaCl2 and stored at −20 °C until used.

Article Title: Structures of closed and open conformations of dimeric human ATM
Article Snippet: Cells were harvested 16 hours later by centrifugation and lysed using a high-pressure homogenizer (Avestin, Biopharma) in a lysis buffer containing 50 mM Hepes (pH 7.4, 4°C), 500 mM NaCl, 5% (v/v) glycerol, 20 mM imidazole, 0.05% (v/v) Tween 20, 8 mM β-mercaptoethanol, EDTA-free protease inhibitor cocktail (Roche), ribonuclease A (Sigma-Aldrich), deoxyribonuclease I (Sigma-Aldrich), and lysozyme (Sigma-Aldrich). .. Finally, the elution fractions were directly subjected to SEC using an XK 26/70 Superose 6 prep grade column (GE Healthcare) equilibrated in 20 mM Hepes (pH 7.3, 4°C), 500 mM NaCl, 200 mM arginine, 10% (v/v) glycerol, and 4 mM DTT.

Cell Culture:

Article Title: Unraveling the Helicobacter pylori UreG zinc binding site using X-ray absorption spectroscopy (XAS) and structural modeling
Article Snippet: Cells were grown in 2 L batches of M9 autoinduction medium at 28 °C for 48 h, starting from 50 mL of preinoculate cell culture that was grown for 16 h. Cells were harvested by centrifugation at 8,000g for 20 min at 4 °C. .. The soluble fraction obtained from the cell lysate was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been preequilibrated with 20 mM Tris–HCl buffer, pH 7.0, containing 2 mM EDTA.

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: The cell culture was then incubated at 37 °C with shaking until it reached an OD600 of 0.6; at this point, protein production was induced by addition of IPTG at a final concentration of 1 mM. .. The proteins were eluted with buffer A supplemented with 500 mM imidazole, pH8, after the column was washed with five bed volumes of buffer A. cMCR-1 and cMCR-2 were further purified by size-exclusion chromatography (SEC) using Superdex 200 - XK 26/70 column (GE Healthcare).

Generated:

Article Title: Familial secondary erythrocytosis due to increased oxygen affinity is caused by destabilization of the T state of hemoglobin Brigham (?2Pro100Leu)
Article Snippet: Catalase was removed from wild-type Hb using an XK 26/100 column containing Superdex-200 chromatography media (GE Healthcare Biosciences, Piscataway, NJ). .. Recombinant hemoglobin Brigham was generated by site-directed mutagenesis of the pHE2 construct that was kindly provided by C. Ho and T.-J.

Protein Concentration:

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: The proteins were eluted with buffer A supplemented with 500 mM imidazole, pH8, after the column was washed with five bed volumes of buffer A. cMCR-1 and cMCR-2 were further purified by size-exclusion chromatography (SEC) using Superdex 200 - XK 26/70 column (GE Healthcare). .. Protein concentration was determined by absorbance measurements at 280 nm using an absorbance coefficient (0.1%) of 1.03 for cMCR-1 and 1.12 for cMCR-2 (ProtParam tool: http://www.expasy.org/tools/ ).

Sequencing:

Article Title: Structures of closed and open conformations of dimeric human ATM
Article Snippet: Fractions containing recombinant protein were pooled, and the N-terminal His6 -lipoyl domain was cleaved off by a tobacco etch virus protease (a Gly-Gly-Ser sequence remains to precede the initial Met of p53 full-length sequence in the final cleavage product). .. Finally, the elution fractions were directly subjected to SEC using an XK 26/70 Superose 6 prep grade column (GE Healthcare) equilibrated in 20 mM Hepes (pH 7.3, 4°C), 500 mM NaCl, 200 mM arginine, 10% (v/v) glycerol, and 4 mM DTT.

Article Title: Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex
Article Snippet: GyrB and GyrA expression and purification The sequence coding for the full-length E . coli GyrA (2-875) was inserted into a modified pET28b containing an N-terminal 10-His tag and a C-terminal Twin-strep tag. .. The GyrA protein was purified by nickel-affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE Healthcare) bound to Ni2+ ions.

Sonication:

Article Title: Expression, purification and crystallization of pecan (Carya illinoinensis) vicilin
Article Snippet: Cells were lysed by sonication for 8 min in a beaker surrounded by a mixture of ice and water using a Model 450 Digital Sonifier (Branson, Danbury, Connecticut, USA). .. The main peak of the HisTrap elution was loaded onto a XK 26/70 Superdex-200 column (GE Healthcare) pre-equilibrated and eluted with 10 m M Tris–HCl pH 7.9, 500 m M NaCl at a flow rate of 1 ml min−1 .

Article Title: Structure and Protein–Protein Interactions of Methanol Dehydrogenase from Methylococcus capsulatus (Bath)
Article Snippet: Purification of MDH from M. capsulatus (Bath) M. capsulatus (Bath) cells (∼20 g) were resuspended in lysis buffer [25 mM PIPES (pH 7.2) and 250 mM NaCl] and sonicated for 10 min (10 s on and 30 s off at 40% amplitude) on ice. .. The supernatant was loaded onto a DEAE-Sepharose FF XK 26/20 column (GE Healthcare) and washed with 20 mM Tris (pH 8.0) until all unbound protein eluted.

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: Cells were ruptured by sonication and the cell lysate was centrifuged for 1 hour at 15,000 × g . .. The proteins were eluted with buffer A supplemented with 500 mM imidazole, pH8, after the column was washed with five bed volumes of buffer A. cMCR-1 and cMCR-2 were further purified by size-exclusion chromatography (SEC) using Superdex 200 - XK 26/70 column (GE Healthcare).

Injection:

Article Title: Polyglycine hydrolases: Fungal β-lactamase-like endoproteases that cleave polyglycine regions within plant class IV chitinases
Article Snippet: .. The clarified extract was injected onto a Capto MMC XK 26/20 column (GE Healthcare, Waukesha WI) resulting in capture of Es-cmp. ..

Recombinant:

Article Title: Structures of closed and open conformations of dimeric human ATM
Article Snippet: Fractions containing recombinant protein were pooled, and the N-terminal His6 -lipoyl domain was cleaved off by a tobacco etch virus protease (a Gly-Gly-Ser sequence remains to precede the initial Met of p53 full-length sequence in the final cleavage product). .. Finally, the elution fractions were directly subjected to SEC using an XK 26/70 Superose 6 prep grade column (GE Healthcare) equilibrated in 20 mM Hepes (pH 7.3, 4°C), 500 mM NaCl, 200 mM arginine, 10% (v/v) glycerol, and 4 mM DTT.

Article Title: Familial secondary erythrocytosis due to increased oxygen affinity is caused by destabilization of the T state of hemoglobin Brigham (?2Pro100Leu)
Article Snippet: Catalase was removed from wild-type Hb using an XK 26/100 column containing Superdex-200 chromatography media (GE Healthcare Biosciences, Piscataway, NJ). .. Recombinant hemoglobin Brigham was generated by site-directed mutagenesis of the pHE2 construct that was kindly provided by C. Ho and T.-J.

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: Paragraph title: Construction, expression and purification of MCR-1 and MCR-2 recombinant proteins ... The proteins were eluted with buffer A supplemented with 500 mM imidazole, pH8, after the column was washed with five bed volumes of buffer A. cMCR-1 and cMCR-2 were further purified by size-exclusion chromatography (SEC) using Superdex 200 - XK 26/70 column (GE Healthcare).

Molecular Weight:

Article Title: Role of the ?1 Subunit in the Function and Stability of the 20S Proteasome in the Hyperthermophilic Archaeon Pyrococcus furiosus ▿
Article Snippet: The soluble protein fraction was applied to a 40-ml Q-Sepharose XK 26/20 column (GE Life Sciences, Piscataway, NJ) and eluted between 0.5 and 0.7 M NaCl. .. Many of the smaller contaminating proteins were cleared from the resulting VKM-MCA active pool using a Microcon centrifugal concentrator of 100,000 molecular weight cutoff (MWCO).

Article Title: Structure and Protein–Protein Interactions of Methanol Dehydrogenase from Methylococcus capsulatus (Bath)
Article Snippet: The supernatant was loaded onto a DEAE-Sepharose FF XK 26/20 column (GE Healthcare) and washed with 20 mM Tris (pH 8.0) until all unbound protein eluted. .. The fractions containing MDH were collected and concentrated using a Centriprep molecular weight cutoff (MWCO) 10 device.

Mass Spectrometry:

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: The proteins were eluted with buffer A supplemented with 500 mM imidazole, pH8, after the column was washed with five bed volumes of buffer A. cMCR-1 and cMCR-2 were further purified by size-exclusion chromatography (SEC) using Superdex 200 - XK 26/70 column (GE Healthcare). .. Mass spectrometry for verification of the full-length recombinant MCR-1 protein was performed by UC Berkeley, Vincent J. Coates Proteomics/Mass Spectrometry Laboratory ( http://qb3.berkeley.edu/pmsl/ ).

Mutagenesis:

Article Title: Familial secondary erythrocytosis due to increased oxygen affinity is caused by destabilization of the T state of hemoglobin Brigham (?2Pro100Leu)
Article Snippet: Catalase was removed from wild-type Hb using an XK 26/100 column containing Superdex-200 chromatography media (GE Healthcare Biosciences, Piscataway, NJ). .. Recombinant hemoglobin Brigham was generated by site-directed mutagenesis of the pHE2 construct that was kindly provided by C. Ho and T.-J.

Isolation:

Article Title: Induction of Apoptosis and Cytotoxicity by Isothiocyanate Sulforaphene in Human Hepatocarcinoma HepG2 Cells
Article Snippet: Paragraph title: 2.1. Isolation of Glucoraphenin (GRE) ... The purity was further improved by gel-filtration removal of contaminants, which was performed using an XK 26/100 column packed with Sephadex G10 chromatography media (Amersham BioSciences, Buckinghamshire, UK), connected to an FPLC System (Pharmacia, Kent, UK).

Article Title: Familial secondary erythrocytosis due to increased oxygen affinity is caused by destabilization of the T state of hemoglobin Brigham (?2Pro100Leu)
Article Snippet: Paragraph title: Hemoglobin isolation ... Catalase was removed from wild-type Hb using an XK 26/100 column containing Superdex-200 chromatography media (GE Healthcare Biosciences, Piscataway, NJ).

Article Title: Unraveling the Helicobacter pylori UreG zinc binding site using X-ray absorption spectroscopy (XAS) and structural modeling
Article Snippet: The protein was isolated using a combination of anion-exchange and size-exclusion chromatographic separations. .. The soluble fraction obtained from the cell lysate was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been preequilibrated with 20 mM Tris–HCl buffer, pH 7.0, containing 2 mM EDTA.

Article Title: Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who’s Leading HCHT’s Biological Function
Article Snippet: .. In this study, isolated ChBDCHIT1-49 domains were purified by molecular exclusion chromatography using XK 26/100 SuperDex 75 PrepGrade column (GE Healthcare) equilibrated in 150 mM NaCl 50 mM phosphate buffer pH 7.5 (PBS). .. Isotopic Labelled Protein Expression and Production for NMR studies 13 C, 15 N or 15 N ChBDCHIT1-49 samples were expressed in E. coli BL21(DE3) cells.

Size-exclusion Chromatography:

Article Title: Structures of closed and open conformations of dimeric human ATM
Article Snippet: .. Finally, the elution fractions were directly subjected to SEC using an XK 26/70 Superose 6 prep grade column (GE Healthcare) equilibrated in 20 mM Hepes (pH 7.3, 4°C), 500 mM NaCl, 200 mM arginine, 10% (v/v) glycerol, and 4 mM DTT. .. The protein was eluted at 3 mg/ml (67 μM), and the aliquots were flash-frozen in liquid nitrogen for storage at −80°C before p53 was used for the kinase assay.

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: .. The proteins were eluted with buffer A supplemented with 500 mM imidazole, pH8, after the column was washed with five bed volumes of buffer A. cMCR-1 and cMCR-2 were further purified by size-exclusion chromatography (SEC) using Superdex 200 - XK 26/70 column (GE Healthcare). .. For cMCR-1, the N-terminal His-tag was removed by tobacco etch virus (TEV) protease before gel filtration.

Purification:

Article Title: Structures of closed and open conformations of dimeric human ATM
Article Snippet: Paragraph title: Human p53 expression and purification for kinase assay ... Finally, the elution fractions were directly subjected to SEC using an XK 26/70 Superose 6 prep grade column (GE Healthcare) equilibrated in 20 mM Hepes (pH 7.3, 4°C), 500 mM NaCl, 200 mM arginine, 10% (v/v) glycerol, and 4 mM DTT.

Article Title: Role of the ?1 Subunit in the Function and Stability of the 20S Proteasome in the Hyperthermophilic Archaeon Pyrococcus furiosus ▿
Article Snippet: Paragraph title: Purification of 20S proteasome from P. furiosus grown at 80°C and 90°C. ... The soluble protein fraction was applied to a 40-ml Q-Sepharose XK 26/20 column (GE Life Sciences, Piscataway, NJ) and eluted between 0.5 and 0.7 M NaCl.

Article Title: Polyglycine hydrolases: Fungal β-lactamase-like endoproteases that cleave polyglycine regions within plant class IV chitinases
Article Snippet: Paragraph title: Purification of Es-cmp ... The clarified extract was injected onto a Capto MMC XK 26/20 column (GE Healthcare, Waukesha WI) resulting in capture of Es-cmp.

Article Title: Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex
Article Snippet: .. The GyrA protein was purified by nickel-affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE Healthcare) bound to Ni2+ ions. .. Elution was performed with the lysis buffer containing 250 mM imidazol pH 8.0 and eluted proteins were directly attached on a 10 ml Streptavidin Sepharose (GE Healthcare).

Article Title: Familial secondary erythrocytosis due to increased oxygen affinity is caused by destabilization of the T state of hemoglobin Brigham (?2Pro100Leu)
Article Snippet: HbA was purified following osmotic lysis and centrifugation within 1 day of collection according to established methods. .. Catalase was removed from wild-type Hb using an XK 26/100 column containing Superdex-200 chromatography media (GE Healthcare Biosciences, Piscataway, NJ).

Article Title: Structure and Protein–Protein Interactions of Methanol Dehydrogenase from Methylococcus capsulatus (Bath)
Article Snippet: Paragraph title: Purification of MDH from M. capsulatus (Bath) ... The supernatant was loaded onto a DEAE-Sepharose FF XK 26/20 column (GE Healthcare) and washed with 20 mM Tris (pH 8.0) until all unbound protein eluted.

Article Title: Unraveling the Helicobacter pylori UreG zinc binding site using X-ray absorption spectroscopy (XAS) and structural modeling
Article Snippet: Paragraph title: Hp UreG expression and purification ... The soluble fraction obtained from the cell lysate was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been preequilibrated with 20 mM Tris–HCl buffer, pH 7.0, containing 2 mM EDTA.

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: .. The proteins were eluted with buffer A supplemented with 500 mM imidazole, pH8, after the column was washed with five bed volumes of buffer A. cMCR-1 and cMCR-2 were further purified by size-exclusion chromatography (SEC) using Superdex 200 - XK 26/70 column (GE Healthcare). .. For cMCR-1, the N-terminal His-tag was removed by tobacco etch virus (TEV) protease before gel filtration.

Article Title: Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who’s Leading HCHT’s Biological Function
Article Snippet: .. In this study, isolated ChBDCHIT1-49 domains were purified by molecular exclusion chromatography using XK 26/100 SuperDex 75 PrepGrade column (GE Healthcare) equilibrated in 150 mM NaCl 50 mM phosphate buffer pH 7.5 (PBS). .. Isotopic Labelled Protein Expression and Production for NMR studies 13 C, 15 N or 15 N ChBDCHIT1-49 samples were expressed in E. coli BL21(DE3) cells.

Protein Purification:

Article Title: The relationship between folding and activity in UreG, an intrinsically disordered enzyme
Article Snippet: Paragraph title: Protein purification ... The supertnatant of the lysis was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been pre-equilibrated with 2 volumes of 20 mM Tris-HCl buffer pH 8, containing 1 mM DTT and 5 mM EDTA.

Article Title: Expression, purification and crystallization of pecan (Carya illinoinensis) vicilin
Article Snippet: Paragraph title: 2.3. Protein purification   ... The main peak of the HisTrap elution was loaded onto a XK 26/70 Superdex-200 column (GE Healthcare) pre-equilibrated and eluted with 10 m M Tris–HCl pH 7.9, 500 m M NaCl at a flow rate of 1 ml min−1 .

Fast Protein Liquid Chromatography:

Article Title: Induction of Apoptosis and Cytotoxicity by Isothiocyanate Sulforaphene in Human Hepatocarcinoma HepG2 Cells
Article Snippet: .. The purity was further improved by gel-filtration removal of contaminants, which was performed using an XK 26/100 column packed with Sephadex G10 chromatography media (Amersham BioSciences, Buckinghamshire, UK), connected to an FPLC System (Pharmacia, Kent, UK). ..

Article Title: Expression, purification and crystallization of pecan (Carya illinoinensis) vicilin
Article Snippet: The main peak of the HisTrap elution was loaded onto a XK 26/70 Superdex-200 column (GE Healthcare) pre-equilibrated and eluted with 10 m M Tris–HCl pH 7.9, 500 m M NaCl at a flow rate of 1 ml min−1 . .. All chromatographic steps were carried out at room temperature using an FPLC system (GE Healthcare).

Lysis:

Article Title: Structures of closed and open conformations of dimeric human ATM
Article Snippet: Cells were harvested 16 hours later by centrifugation and lysed using a high-pressure homogenizer (Avestin, Biopharma) in a lysis buffer containing 50 mM Hepes (pH 7.4, 4°C), 500 mM NaCl, 5% (v/v) glycerol, 20 mM imidazole, 0.05% (v/v) Tween 20, 8 mM β-mercaptoethanol, EDTA-free protease inhibitor cocktail (Roche), ribonuclease A (Sigma-Aldrich), deoxyribonuclease I (Sigma-Aldrich), and lysozyme (Sigma-Aldrich). .. Finally, the elution fractions were directly subjected to SEC using an XK 26/70 Superose 6 prep grade column (GE Healthcare) equilibrated in 20 mM Hepes (pH 7.3, 4°C), 500 mM NaCl, 200 mM arginine, 10% (v/v) glycerol, and 4 mM DTT.

Article Title: Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex
Article Snippet: Cells were induced with 0.35 mM IPTG after reaching an OD600 0.85 and protein was expressed at 37 °C for 4 h. Cells were harvested and resuspended in lysis buffer (20 mM Hepes, 500 mM NaCl, 20 mM imidazole, 10% v/v glycerol, pH 8.0) and lysed with three cycles of high-pressure disruption using EmulsiFlex-C3 at 1500 bars. .. The GyrA protein was purified by nickel-affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE Healthcare) bound to Ni2+ ions.

Article Title: The relationship between folding and activity in UreG, an intrinsically disordered enzyme
Article Snippet: .. The supertnatant of the lysis was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been pre-equilibrated with 2 volumes of 20 mM Tris-HCl buffer pH 8, containing 1 mM DTT and 5 mM EDTA. ..

Article Title: Familial secondary erythrocytosis due to increased oxygen affinity is caused by destabilization of the T state of hemoglobin Brigham (?2Pro100Leu)
Article Snippet: HbA was purified following osmotic lysis and centrifugation within 1 day of collection according to established methods. .. Catalase was removed from wild-type Hb using an XK 26/100 column containing Superdex-200 chromatography media (GE Healthcare Biosciences, Piscataway, NJ).

Article Title: Structure and Protein–Protein Interactions of Methanol Dehydrogenase from Methylococcus capsulatus (Bath)
Article Snippet: Purification of MDH from M. capsulatus (Bath) M. capsulatus (Bath) cells (∼20 g) were resuspended in lysis buffer [25 mM PIPES (pH 7.2) and 250 mM NaCl] and sonicated for 10 min (10 s on and 30 s off at 40% amplitude) on ice. .. The supernatant was loaded onto a DEAE-Sepharose FF XK 26/20 column (GE Healthcare) and washed with 20 mM Tris (pH 8.0) until all unbound protein eluted.

SDS Page:

Article Title: Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who’s Leading HCHT’s Biological Function
Article Snippet: Protein purity level and homogeneity were confirmed by SDS-PAGE and UV-Visible (125–400 nm) spectra. .. In this study, isolated ChBDCHIT1-49 domains were purified by molecular exclusion chromatography using XK 26/100 SuperDex 75 PrepGrade column (GE Healthcare) equilibrated in 150 mM NaCl 50 mM phosphate buffer pH 7.5 (PBS).

Affinity Chromatography:

Article Title: Polyglycine hydrolases: Fungal β-lactamase-like endoproteases that cleave polyglycine regions within plant class IV chitinases
Article Snippet: The clarified extract was injected onto a Capto MMC XK 26/20 column (GE Healthcare, Waukesha WI) resulting in capture of Es-cmp. .. Eluted Es-cmp was further purified and concentrated by lectin affinity chromatography (HiTrap ConA 4B, 1 ml, GE Healthcare).

Transformation Assay:

Article Title: The relationship between folding and activity in UreG, an intrinsically disordered enzyme
Article Snippet: E. coli BL21(DE3) cells transformed with pET3a-SpureG construct were grown at 37 °C with vigorous stirring. .. The supertnatant of the lysis was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been pre-equilibrated with 2 volumes of 20 mM Tris-HCl buffer pH 8, containing 1 mM DTT and 5 mM EDTA.

Protein Binding:

Article Title: Dataset on protein composition of a human plasma sub-proteome able to modulate the Dengue 2 virus infection in Huh 7.5 cells
Article Snippet: .. The resulting sample was loaded at a flow rate of 10 cm/h onto an XK-26 chromatography column (GE Healthcare, USA) packed with 10 mL of DE-52 gel, which was next washed with equilibration buffer until absorbance at 280 nm returned to baseline (bound protein represented, in average, 1.2% of the initial protein contents of the sample and no more than 60% of the total protein binding capacity estimated for DE-52 at pH 6.0). ..

Concentration Assay:

Article Title: Novel Mechanism of Hemin Capture by Hbp2, the Hemoglobin-binding Hemophore from Listeria monocytogenes *
Article Snippet: .. After concentration, the protein was then applied onto a Sephacryl S-100 XK 26/100 gel filtration column (GE Healthcare) equilibrated with buffer B, and pure fractions were pooled for crystallography trials. .. The Hbp2N2 -hemin complex was concentrated to 53 mg/ml and crystallized at room temperature using the hanging drop vapor diffusion method in a reservoir solution of 100 m m MES, pH 6.0, 10 m m ZnCl2 , 20% polyethylene glycol 6000.

Article Title: The relationship between folding and activity in UreG, an intrinsically disordered enzyme
Article Snippet: When OD600 reached 0.5–0.6, expression was induced by addition of isopropyl β-thiogalactopyranoside (IPTG) to a final concentration of 0.5 mM, and the temperature was decreased to 20 °C. .. The supertnatant of the lysis was loaded onto a Q-Sepharose XK 26/10 column (GE Healthcare) that had been pre-equilibrated with 2 volumes of 20 mM Tris-HCl buffer pH 8, containing 1 mM DTT and 5 mM EDTA.

Article Title: Structure and Protein–Protein Interactions of Methanol Dehydrogenase from Methylococcus capsulatus (Bath)
Article Snippet: The supernatant was loaded onto a DEAE-Sepharose FF XK 26/20 column (GE Healthcare) and washed with 20 mM Tris (pH 8.0) until all unbound protein eluted. .. After concentration, MDH was loaded onto a HiLoad Superdex 75 16/600 prep grade column (GE Healthcare) pre-equilibrated with 20 mM Tris (pH 8.0) and 300 mM NaCl.

Article Title: Development of novel antibodies for detection of mobile colistin-resistant bacteria contaminated in meats
Article Snippet: The cell culture was then incubated at 37 °C with shaking until it reached an OD600 of 0.6; at this point, protein production was induced by addition of IPTG at a final concentration of 1 mM. .. The proteins were eluted with buffer A supplemented with 500 mM imidazole, pH8, after the column was washed with five bed volumes of buffer A. cMCR-1 and cMCR-2 were further purified by size-exclusion chromatography (SEC) using Superdex 200 - XK 26/70 column (GE Healthcare).

Fractionation:

Article Title: Dataset on protein composition of a human plasma sub-proteome able to modulate the Dengue 2 virus infection in Huh 7.5 cells
Article Snippet: Paragraph title: Fractionation of human plasma samples ... The resulting sample was loaded at a flow rate of 10 cm/h onto an XK-26 chromatography column (GE Healthcare, USA) packed with 10 mL of DE-52 gel, which was next washed with equilibration buffer until absorbance at 280 nm returned to baseline (bound protein represented, in average, 1.2% of the initial protein contents of the sample and no more than 60% of the total protein binding capacity estimated for DE-52 at pH 6.0).

Staining:

Article Title: Expression, purification and crystallization of pecan (Carya illinoinensis) vicilin
Article Snippet: The main peak of the HisTrap elution was loaded onto a XK 26/70 Superdex-200 column (GE Healthcare) pre-equilibrated and eluted with 10 m M Tris–HCl pH 7.9, 500 m M NaCl at a flow rate of 1 ml min−1 . .. The gels were stained using the Colloidal Coomassie G-250 Staining protocols (Dyballa & Metzger, 2009 ).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    GE Healthcare superdex 75 column
    Purification of MTC28-BAP protein. Chromatogram showing (A) Elution profile of H10-T-MTC28-BAP protein on Ni Sepharose Fast Flow (NiFF) affinity column. Fraction numbers 6–17 were pooled (NiFF pool). (B) Elution profile of H10-T-MTC28-BAP protein on <t>Superdex</t> 75 gel-filtration column. Fraction numbers 16–24 were pooled (GFC pool). The GFC pool was treated with H6-TEV protease to cleave H10 tag from the protein followed by removal of cleaved tag and H6-TEV protease using Ni-affinity chromatography. (C) Elution profile of MTC28-BAP protein on Q Sepharose HP column. Fraction numbers 22–27 were pooled (QHP pool). (D) SDS-PAGE analysis of H10-T-MTC28-BAP protein at different stages during purification. The samples were analyzed by 0.1% SDS-12.5% PAGE under reducing conditions. The protein bands were visualized with Coomassie brilliant blue R-250 staining. Lane M, molecular weight marker, broad range (Bio-Rad, Hercules, CA) (shown in kDa); Lane 1, total cell after homogenization; Lane 2, High-High Speed Supernatant; Lane 3, NiFF pool; Lane 4, GFC pool; Lane 5, NiFF-TT pool (after desalting); Lane 6, QHP pool.
    Superdex 75 Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 859 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superdex 75 column/product/GE Healthcare
    Average 99 stars, based on 859 article reviews
    Price from $9.99 to $1999.99
    superdex 75 column - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    79
    GE Healthcare empty chromatography columns xk 26 40
    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.
    Empty Chromatography Columns Xk 26 40, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/empty chromatography columns xk 26 40/product/GE Healthcare
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    empty chromatography columns xk 26 40 - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    77
    GE Healthcare xk 26 40 columns
    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.
    Xk 26 40 Columns, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xk 26 40 columns/product/GE Healthcare
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xk 26 40 columns - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    99
    GE Healthcare superdex 200
    Panel A: Non-denaturing PAGE analysis showing a much greater mobility of apoLp-III compared to apoA-I and the chimera. Twenty μg of protein was electrophoresed in the absence of SDS on a 4–20% Tris-glycine gel. Lane 1: apoA-I, lane 2: apoLp-III cys /CT-apoA-I, lane 3: apoLp-III. Panel B: Size-exclusion chromatographic analysis. Protein (0.5 mg at a 1 mg/mL concentration) was applied to a <t>Superdex-200</t> column. Elution of the proteins was monitored at 210 nm using a flow rate of 0.5 mL/min. ApoLp-III eluted as a single peak at 17 mL (dash-dotted line), while apoA-I (solid line) and the chimera (dotted line) elute much earlier at 11 mL.
    Superdex 200, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 856 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superdex 200/product/GE Healthcare
    Average 99 stars, based on 856 article reviews
    Price from $9.99 to $1999.99
    superdex 200 - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    Purification of MTC28-BAP protein. Chromatogram showing (A) Elution profile of H10-T-MTC28-BAP protein on Ni Sepharose Fast Flow (NiFF) affinity column. Fraction numbers 6–17 were pooled (NiFF pool). (B) Elution profile of H10-T-MTC28-BAP protein on Superdex 75 gel-filtration column. Fraction numbers 16–24 were pooled (GFC pool). The GFC pool was treated with H6-TEV protease to cleave H10 tag from the protein followed by removal of cleaved tag and H6-TEV protease using Ni-affinity chromatography. (C) Elution profile of MTC28-BAP protein on Q Sepharose HP column. Fraction numbers 22–27 were pooled (QHP pool). (D) SDS-PAGE analysis of H10-T-MTC28-BAP protein at different stages during purification. The samples were analyzed by 0.1% SDS-12.5% PAGE under reducing conditions. The protein bands were visualized with Coomassie brilliant blue R-250 staining. Lane M, molecular weight marker, broad range (Bio-Rad, Hercules, CA) (shown in kDa); Lane 1, total cell after homogenization; Lane 2, High-High Speed Supernatant; Lane 3, NiFF pool; Lane 4, GFC pool; Lane 5, NiFF-TT pool (after desalting); Lane 6, QHP pool.

    Journal: PLoS ONE

    Article Title: Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection

    doi: 10.1371/journal.pone.0191315

    Figure Lengend Snippet: Purification of MTC28-BAP protein. Chromatogram showing (A) Elution profile of H10-T-MTC28-BAP protein on Ni Sepharose Fast Flow (NiFF) affinity column. Fraction numbers 6–17 were pooled (NiFF pool). (B) Elution profile of H10-T-MTC28-BAP protein on Superdex 75 gel-filtration column. Fraction numbers 16–24 were pooled (GFC pool). The GFC pool was treated with H6-TEV protease to cleave H10 tag from the protein followed by removal of cleaved tag and H6-TEV protease using Ni-affinity chromatography. (C) Elution profile of MTC28-BAP protein on Q Sepharose HP column. Fraction numbers 22–27 were pooled (QHP pool). (D) SDS-PAGE analysis of H10-T-MTC28-BAP protein at different stages during purification. The samples were analyzed by 0.1% SDS-12.5% PAGE under reducing conditions. The protein bands were visualized with Coomassie brilliant blue R-250 staining. Lane M, molecular weight marker, broad range (Bio-Rad, Hercules, CA) (shown in kDa); Lane 1, total cell after homogenization; Lane 2, High-High Speed Supernatant; Lane 3, NiFF pool; Lane 4, GFC pool; Lane 5, NiFF-TT pool (after desalting); Lane 6, QHP pool.

    Article Snippet: For H10-T-MTC28-BAP protein, gel-filtration chromatography of NiFF pool was performed on 480 ml Superdex 75 column (XK 26/100, GE Healthcare).

    Techniques: Purification, Flow Cytometry, Affinity Column, Filtration, Affinity Chromatography, SDS Page, Polyacrylamide Gel Electrophoresis, Staining, Molecular Weight, Marker, Homogenization

    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Article Snippet: Empty chromatography columns XK 26/40 (400 mm in length, 26 mm I.D., GE Healthcare, Piscataway, NJ) were packed with the anion exchange resin Q-sepharose XL (GE Healthcare).

    Techniques: Purification, Injection, Generated

    Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Article Snippet: Empty chromatography columns XK 26/40 (400 mm in length, 26 mm I.D., GE Healthcare, Piscataway, NJ) were packed with the anion exchange resin Q-sepharose XL (GE Healthcare).

    Techniques: Purification, Injection, Generated

    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Article Snippet: XK 26/40 columns (GE Healthcare) were packed with Q-sepharose XL resin (GE Healthcare) at room temperature using the ethanol-slurry-packing technique [ ].

    Techniques: Purification, Injection, Generated

    Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Article Snippet: XK 26/40 columns (GE Healthcare) were packed with Q-sepharose XL resin (GE Healthcare) at room temperature using the ethanol-slurry-packing technique [ ].

    Techniques: Purification, Injection, Generated

    Panel A: Non-denaturing PAGE analysis showing a much greater mobility of apoLp-III compared to apoA-I and the chimera. Twenty μg of protein was electrophoresed in the absence of SDS on a 4–20% Tris-glycine gel. Lane 1: apoA-I, lane 2: apoLp-III cys /CT-apoA-I, lane 3: apoLp-III. Panel B: Size-exclusion chromatographic analysis. Protein (0.5 mg at a 1 mg/mL concentration) was applied to a Superdex-200 column. Elution of the proteins was monitored at 210 nm using a flow rate of 0.5 mL/min. ApoLp-III eluted as a single peak at 17 mL (dash-dotted line), while apoA-I (solid line) and the chimera (dotted line) elute much earlier at 11 mL.

    Journal: Biochimica et biophysica acta

    Article Title: Transfer of C-terminal residues of human apolipoprotein A-I to insect apolipophorin III creates a two-domain chimeric protein with enhanced lipid binding activity

    doi: 10.1016/j.bbamem.2017.04.017

    Figure Lengend Snippet: Panel A: Non-denaturing PAGE analysis showing a much greater mobility of apoLp-III compared to apoA-I and the chimera. Twenty μg of protein was electrophoresed in the absence of SDS on a 4–20% Tris-glycine gel. Lane 1: apoA-I, lane 2: apoLp-III cys /CT-apoA-I, lane 3: apoLp-III. Panel B: Size-exclusion chromatographic analysis. Protein (0.5 mg at a 1 mg/mL concentration) was applied to a Superdex-200 column. Elution of the proteins was monitored at 210 nm using a flow rate of 0.5 mL/min. ApoLp-III eluted as a single peak at 17 mL (dash-dotted line), while apoA-I (solid line) and the chimera (dotted line) elute much earlier at 11 mL.

    Article Snippet: The protein solution was transferred to 6–8 kDa cut-off dialysis tubes (Spectrum laboratories, Rancho Dominguez, CA) and dialyzed against 4 L of 10 mM ammonium bicarbonate, pH 7.8 containing 1 mM EDTA with 3 additional buffer changes within 48 h. The recombinant proteins were further purified by size-exclusion chromatography using Superdex 200 (XK-26/70 column, GE Healthcare, Pittsburgh, PA).

    Techniques: Polyacrylamide Gel Electrophoresis, Concentration Assay, Flow Cytometry