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GE Healthcare xk 26 column
Xk 26 Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xk 26 column/product/GE Healthcare
Average 89 stars, based on 5 article reviews
Price from $9.99 to $1999.99
xk 26 column - by Bioz Stars, 2020-01
89/100 stars

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Centrifugation:

Article Title: Structural Characterization of Proline-rich Tyrosine Kinase 2 (PYK2) Reveals a Unique (DFG-out) Conformation and Enables Inhibitor Design
Article Snippet: The lysate was clarified by centrifugation at 4 °C for 35 min at 30,000 × g (16,000 rpm) in a Sorval SL-50T (or appropriate) rotor. .. The resin was then transferred to an XK-26 column (Amersham Biosciences) connected to a FPLC™ and washed with buffer B until the OD reached 0.05 units.

Article Title: Three Different Classes of Aminotransferases Evolved Prephenate Aminotransferase Functionality in Arogenate-competent Microorganisms *
Article Snippet: Cellswere harvested by centrifugation (4000 × g , 45 min), and pellets were resuspended in 30 ml of 25 m m Hepes, pH 8.0, 1 m m EDTA, 1 m m DTT, 10% glycerol (v/v), 50 μ m PLP, 5 m m ϵ-aminocaproic acid, and 1 m m benzamidine and sonicated for 10 min at 4 °C on a Branson sonicator. .. Crude extract was loaded onto 130 ml of EMD DEAE 650(M) resin (Merck) in an XK 26 column (26 × 260 mm2 , Amersham Biosciences) equilibrated with buffer A: 25 m m Hepes, pH 8.0, 1 m m EDTA, 1 m m DTT, 10% glycerol (v/v), 50 μ m PLP.

Article Title: A Plant-Produced Pfs230 Vaccine Candidate Blocks Transmission of Plasmodium falciparum ▿ ▿ †
Article Snippet: Following incubation, the plant homogenate was clarified by centrifugation (15,000 × g for 45 min). .. The filtrate was loaded onto an XK 26 column with Chelating Sepharose Big Beads resin (70-ml column volume [CV]) using the ÄKTA Purifier 100 (GE Healthcare, Piscataway, NJ) at a flow rate of 25 ml/min.

Article Title: Nucleosome Recognition by the Piccolo NuA4 Histone Acetyltransferase Complex †
Article Snippet: Soluble protein was collected by centrifugation for 20 min at 14000 rpm. .. Protein was further purified by size-exclusion chromatography on an XK-26 column packed with Sephracryl 200 (Amersham Biosciences) in 50 mM Tris at pH 7.5, 300 mM NaCl, 5 mM β -ME, and 1 mM ethylenediaminetetraacetic acid (EDTA).

Filtration:

Article Title: A Plant-Produced Pfs230 Vaccine Candidate Blocks Transmission of Plasmodium falciparum ▿ ▿ †
Article Snippet: BioLife filtration capsule (Cuno; 3M, Meriden, CT) and a 0.2-μm Sartopore filter cartridge (Sartorius, Bohemia, NY). .. The filtrate was loaded onto an XK 26 column with Chelating Sepharose Big Beads resin (70-ml column volume [CV]) using the ÄKTA Purifier 100 (GE Healthcare, Piscataway, NJ) at a flow rate of 25 ml/min.

Plasmid Purification:

Article Title: Three Different Classes of Aminotransferases Evolved Prephenate Aminotransferase Functionality in Arogenate-competent Microorganisms *
Article Snippet: .. Crude extract was loaded onto 130 ml of EMD DEAE 650(M) resin (Merck) in an XK 26 column (26 × 260 mm2 , Amersham Biosciences) equilibrated with buffer A: 25 m m Hepes, pH 8.0, 1 m m EDTA, 1 m m DTT, 10% glycerol (v/v), 50 μ m PLP. ..

Incubation:

Article Title: Structural Characterization of Proline-rich Tyrosine Kinase 2 (PYK2) Reveals a Unique (DFG-out) Conformation and Enables Inhibitor Design
Article Snippet: The suspension was incubated at 4 °C for 3 h, with gentle rocking, then centrifuged at 700 × g in a swinging bucket rotor for 2-4 min, and the supernatant was extracted. .. The resin was then transferred to an XK-26 column (Amersham Biosciences) connected to a FPLC™ and washed with buffer B until the OD reached 0.05 units.

Article Title: A Plant-Produced Pfs230 Vaccine Candidate Blocks Transmission of Plasmodium falciparum ▿ ▿ †
Article Snippet: Following incubation, the plant homogenate was clarified by centrifugation (15,000 × g for 45 min). .. The filtrate was loaded onto an XK 26 column with Chelating Sepharose Big Beads resin (70-ml column volume [CV]) using the ÄKTA Purifier 100 (GE Healthcare, Piscataway, NJ) at a flow rate of 25 ml/min.

Activity Assay:

Article Title: The cytosolic entry of diphtheria toxin catalytic domain requires a host cell cytosolic translocation factor complex
Article Snippet: Fractions containing in vitro translocation activity eluted between 150 to 190 mM NaCl, and were pooled and concentrated using Centriplus Centrifugal Filters (YM-10; Amicon) according to manufacturer's directions. .. Next, CTFs were fractionated by size exclusion chromatography using Sephacryl® S200 (Amersham Biosciences) XK 26 column (Amersham Biosciences) equilibrated with buffer B3.

Article Title: Nucleosome Recognition by the Piccolo NuA4 Histone Acetyltransferase Complex †
Article Snippet: Fractions containing Esa1 as determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and activity assays were pooled and concentrated to less than 10 mL. .. Protein was further purified by size-exclusion chromatography on an XK-26 column packed with Sephracryl 200 (Amersham Biosciences) in 50 mM Tris at pH 7.5, 300 mM NaCl, 5 mM β -ME, and 1 mM ethylenediaminetetraacetic acid (EDTA).

Expressing:

Article Title: Nucleosome Recognition by the Piccolo NuA4 Histone Acetyltransferase Complex †
Article Snippet: Protein was further purified by size-exclusion chromatography on an XK-26 column packed with Sephracryl 200 (Amersham Biosciences) in 50 mM Tris at pH 7.5, 300 mM NaCl, 5 mM β -ME, and 1 mM ethylenediaminetetraacetic acid (EDTA). .. To purify the picNuA4 complex, Esa1, Yng2, and Epl1 were coexpressed using the polycistronic expression system developed by Tan and co-workers ( ).

Translocation Assay:

Article Title: The cytosolic entry of diphtheria toxin catalytic domain requires a host cell cytosolic translocation factor complex
Article Snippet: Fractions containing in vitro translocation activity eluted between 150 to 190 mM NaCl, and were pooled and concentrated using Centriplus Centrifugal Filters (YM-10; Amicon) according to manufacturer's directions. .. Next, CTFs were fractionated by size exclusion chromatography using Sephacryl® S200 (Amersham Biosciences) XK 26 column (Amersham Biosciences) equilibrated with buffer B3.

Flow Cytometry:

Article Title: The cytosolic entry of diphtheria toxin catalytic domain requires a host cell cytosolic translocation factor complex
Article Snippet: CTFs were eluted with a linear gradient, 0–400 mM NaCl, in buffer B3 at a flow rate of 5 ml/min. .. Next, CTFs were fractionated by size exclusion chromatography using Sephacryl® S200 (Amersham Biosciences) XK 26 column (Amersham Biosciences) equilibrated with buffer B3.

Article Title: A Plant-Produced Pfs230 Vaccine Candidate Blocks Transmission of Plasmodium falciparum ▿ ▿ †
Article Snippet: .. The filtrate was loaded onto an XK 26 column with Chelating Sepharose Big Beads resin (70-ml column volume [CV]) using the ÄKTA Purifier 100 (GE Healthcare, Piscataway, NJ) at a flow rate of 25 ml/min. ..

Chromatography:

Article Title: Rapid and Scalable Plant-based Production of a Cholera Toxin B Subunit Variant to Aid in Mass Vaccination against Cholera Outbreaks
Article Snippet: Chromatography was performed using an AKTA Purifier (GE Healthcare). .. Talon Superflow Metal Affinity Resin (Clontech), packed in an XK-26 column (GE Healthcare) to a 50 ml bed volume, was equilibrated with 10 column volumes (CV) of buffer A (20 mM Tris-Cl, pH 8.0, 500 mM NaCl).

Article Title: The cytosolic entry of diphtheria toxin catalytic domain requires a host cell cytosolic translocation factor complex
Article Snippet: Next, CTFs were fractionated by size exclusion chromatography using Sephacryl® S200 (Amersham Biosciences) XK 26 column (Amersham Biosciences) equilibrated with buffer B3. .. A Single Path Monitor (UV-1; Amersham Biosciences) was used to monitor chromatography.

Sonication:

Article Title: Three Different Classes of Aminotransferases Evolved Prephenate Aminotransferase Functionality in Arogenate-competent Microorganisms *
Article Snippet: Cellswere harvested by centrifugation (4000 × g , 45 min), and pellets were resuspended in 30 ml of 25 m m Hepes, pH 8.0, 1 m m EDTA, 1 m m DTT, 10% glycerol (v/v), 50 μ m PLP, 5 m m ϵ-aminocaproic acid, and 1 m m benzamidine and sonicated for 10 min at 4 °C on a Branson sonicator. .. Crude extract was loaded onto 130 ml of EMD DEAE 650(M) resin (Merck) in an XK 26 column (26 × 260 mm2 , Amersham Biosciences) equilibrated with buffer A: 25 m m Hepes, pH 8.0, 1 m m EDTA, 1 m m DTT, 10% glycerol (v/v), 50 μ m PLP.

Injection:

Article Title: Silicone Oil Microdroplets Can Induce Antibody Responses Against Recombinant Murine Growth Hormone In Mice
Article Snippet: Materials purchased from Fisher Scientific (Waltham, Massachusetts) included acrylamide, urea, ampicillin sodium salt, isopropyl β-D-1-thiogalactopyranoside (IPTG), reduced glutathione, sodium chloride, sodium hydroxide, tris, Tween 20® , 10 x phosphate buffered saline (PBS), sulfuric acid, HyClone™ water for injection (WFI), and microhematocrit capillary tubes for mouse blood collection. .. Phenyl Sepharose™ High Performance resin was purchased from Fisher Scientific (Waltham, Massachusetts) and packed in a XK 26 column (GE Healthcare Bio-Sciences, Piscataway, New Jersey).

Nucleic Acid Electrophoresis:

Article Title: Nucleosome Recognition by the Piccolo NuA4 Histone Acetyltransferase Complex †
Article Snippet: Fractions containing Esa1 as determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and activity assays were pooled and concentrated to less than 10 mL. .. Protein was further purified by size-exclusion chromatography on an XK-26 column packed with Sephracryl 200 (Amersham Biosciences) in 50 mM Tris at pH 7.5, 300 mM NaCl, 5 mM β -ME, and 1 mM ethylenediaminetetraacetic acid (EDTA).

CtB Assay:

Article Title: Rapid and Scalable Plant-based Production of a Cholera Toxin B Subunit Variant to Aid in Mass Vaccination against Cholera Outbreaks
Article Snippet: Paragraph title: Purification of CTB ... Talon Superflow Metal Affinity Resin (Clontech), packed in an XK-26 column (GE Healthcare) to a 50 ml bed volume, was equilibrated with 10 column volumes (CV) of buffer A (20 mM Tris-Cl, pH 8.0, 500 mM NaCl).

Size-exclusion Chromatography:

Article Title: The cytosolic entry of diphtheria toxin catalytic domain requires a host cell cytosolic translocation factor complex
Article Snippet: .. Next, CTFs were fractionated by size exclusion chromatography using Sephacryl® S200 (Amersham Biosciences) XK 26 column (Amersham Biosciences) equilibrated with buffer B3. .. A Single Path Monitor (UV-1; Amersham Biosciences) was used to monitor chromatography.

Article Title: Nucleosome Recognition by the Piccolo NuA4 Histone Acetyltransferase Complex †
Article Snippet: .. Protein was further purified by size-exclusion chromatography on an XK-26 column packed with Sephracryl 200 (Amersham Biosciences) in 50 mM Tris at pH 7.5, 300 mM NaCl, 5 mM β -ME, and 1 mM ethylenediaminetetraacetic acid (EDTA). .. Fractions containing Esa1 based on SDS–PAGE and activity assays were pooled and concentrated.

Purification:

Article Title: Rapid and Scalable Plant-based Production of a Cholera Toxin B Subunit Variant to Aid in Mass Vaccination against Cholera Outbreaks
Article Snippet: Paragraph title: Purification of CTB ... Talon Superflow Metal Affinity Resin (Clontech), packed in an XK-26 column (GE Healthcare) to a 50 ml bed volume, was equilibrated with 10 column volumes (CV) of buffer A (20 mM Tris-Cl, pH 8.0, 500 mM NaCl).

Article Title: The cytosolic entry of diphtheria toxin catalytic domain requires a host cell cytosolic translocation factor complex
Article Snippet: Paragraph title: Purification of HUT102/6TG CTF complex ... Next, CTFs were fractionated by size exclusion chromatography using Sephacryl® S200 (Amersham Biosciences) XK 26 column (Amersham Biosciences) equilibrated with buffer B3.

Article Title: A Plant-Produced Pfs230 Vaccine Candidate Blocks Transmission of Plasmodium falciparum ▿ ▿ †
Article Snippet: Paragraph title: Purification. ... The filtrate was loaded onto an XK 26 column with Chelating Sepharose Big Beads resin (70-ml column volume [CV]) using the ÄKTA Purifier 100 (GE Healthcare, Piscataway, NJ) at a flow rate of 25 ml/min.

Article Title: Nucleosome Recognition by the Piccolo NuA4 Histone Acetyltransferase Complex †
Article Snippet: .. Protein was further purified by size-exclusion chromatography on an XK-26 column packed with Sephracryl 200 (Amersham Biosciences) in 50 mM Tris at pH 7.5, 300 mM NaCl, 5 mM β -ME, and 1 mM ethylenediaminetetraacetic acid (EDTA). .. Fractions containing Esa1 based on SDS–PAGE and activity assays were pooled and concentrated.

Protein Purification:

Article Title: Three Different Classes of Aminotransferases Evolved Prephenate Aminotransferase Functionality in Arogenate-competent Microorganisms *
Article Snippet: Paragraph title: Preparation of Crude Extracts and Protein Purification ... Crude extract was loaded onto 130 ml of EMD DEAE 650(M) resin (Merck) in an XK 26 column (26 × 260 mm2 , Amersham Biosciences) equilibrated with buffer A: 25 m m Hepes, pH 8.0, 1 m m EDTA, 1 m m DTT, 10% glycerol (v/v), 50 μ m PLP.

Fast Protein Liquid Chromatography:

Article Title: Structural Characterization of Proline-rich Tyrosine Kinase 2 (PYK2) Reveals a Unique (DFG-out) Conformation and Enables Inhibitor Design
Article Snippet: .. The resin was then transferred to an XK-26 column (Amersham Biosciences) connected to a FPLC™ and washed with buffer B until the OD reached 0.05 units. ..

SDS Page:

Article Title: Nucleosome Recognition by the Piccolo NuA4 Histone Acetyltransferase Complex †
Article Snippet: Fractions containing Esa1 as determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and activity assays were pooled and concentrated to less than 10 mL. .. Protein was further purified by size-exclusion chromatography on an XK-26 column packed with Sephracryl 200 (Amersham Biosciences) in 50 mM Tris at pH 7.5, 300 mM NaCl, 5 mM β -ME, and 1 mM ethylenediaminetetraacetic acid (EDTA).

Affinity Chromatography:

Article Title: A Plant-Produced Pfs230 Vaccine Candidate Blocks Transmission of Plasmodium falciparum ▿ ▿ †
Article Snippet: The filtrate was loaded onto an XK 26 column with Chelating Sepharose Big Beads resin (70-ml column volume [CV]) using the ÄKTA Purifier 100 (GE Healthcare, Piscataway, NJ) at a flow rate of 25 ml/min. .. Immobilized metal affinity chromatography (IMAC) eluant was dialyzed into 10 mM sodium phosphate (pH 7.0)–10% glycerol, and the resulting material was centrifuged at 78,000 × g for 10 min to remove the potential insoluble material.

In Vitro:

Article Title: The cytosolic entry of diphtheria toxin catalytic domain requires a host cell cytosolic translocation factor complex
Article Snippet: Fractions containing in vitro translocation activity eluted between 150 to 190 mM NaCl, and were pooled and concentrated using Centriplus Centrifugal Filters (YM-10; Amicon) according to manufacturer's directions. .. Next, CTFs were fractionated by size exclusion chromatography using Sephacryl® S200 (Amersham Biosciences) XK 26 column (Amersham Biosciences) equilibrated with buffer B3.

Concentration Assay:

Article Title: Nucleosome Recognition by the Piccolo NuA4 Histone Acetyltransferase Complex †
Article Snippet: Protein was further purified by size-exclusion chromatography on an XK-26 column packed with Sephracryl 200 (Amersham Biosciences) in 50 mM Tris at pH 7.5, 300 mM NaCl, 5 mM β -ME, and 1 mM ethylenediaminetetraacetic acid (EDTA). .. The final concentration of Esa1 was determined by the method of Bradford ( ).

Lysis:

Article Title: Nucleosome Recognition by the Piccolo NuA4 Histone Acetyltransferase Complex †
Article Snippet: The protein was eluted by a linear gradient from 0 to 200 mM imidazole in lysis buffer. .. Protein was further purified by size-exclusion chromatography on an XK-26 column packed with Sephracryl 200 (Amersham Biosciences) in 50 mM Tris at pH 7.5, 300 mM NaCl, 5 mM β -ME, and 1 mM ethylenediaminetetraacetic acid (EDTA).

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  • 99
    GE Healthcare superdex 75 column
    Purification of MTC28-BAP protein. Chromatogram showing (A) Elution profile of H10-T-MTC28-BAP protein on Ni Sepharose Fast Flow (NiFF) affinity column. Fraction numbers 6–17 were pooled (NiFF pool). (B) Elution profile of H10-T-MTC28-BAP protein on <t>Superdex</t> 75 gel-filtration column. Fraction numbers 16–24 were pooled (GFC pool). The GFC pool was treated with H6-TEV protease to cleave H10 tag from the protein followed by removal of cleaved tag and H6-TEV protease using Ni-affinity chromatography. (C) Elution profile of MTC28-BAP protein on Q Sepharose HP column. Fraction numbers 22–27 were pooled (QHP pool). (D) SDS-PAGE analysis of H10-T-MTC28-BAP protein at different stages during purification. The samples were analyzed by 0.1% SDS-12.5% PAGE under reducing conditions. The protein bands were visualized with Coomassie brilliant blue R-250 staining. Lane M, molecular weight marker, broad range (Bio-Rad, Hercules, CA) (shown in kDa); Lane 1, total cell after homogenization; Lane 2, High-High Speed Supernatant; Lane 3, NiFF pool; Lane 4, GFC pool; Lane 5, NiFF-TT pool (after desalting); Lane 6, QHP pool.
    Superdex 75 Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 859 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superdex 75 column/product/GE Healthcare
    Average 99 stars, based on 859 article reviews
    Price from $9.99 to $1999.99
    superdex 75 column - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    79
    GE Healthcare empty chromatography columns xk 26 40
    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.
    Empty Chromatography Columns Xk 26 40, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/empty chromatography columns xk 26 40/product/GE Healthcare
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    empty chromatography columns xk 26 40 - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    77
    GE Healthcare xk 26 40 columns
    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.
    Xk 26 40 Columns, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xk 26 40 columns/product/GE Healthcare
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xk 26 40 columns - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    99
    GE Healthcare superdex 200
    Panel A: Non-denaturing PAGE analysis showing a much greater mobility of apoLp-III compared to apoA-I and the chimera. Twenty μg of protein was electrophoresed in the absence of SDS on a 4–20% Tris-glycine gel. Lane 1: apoA-I, lane 2: apoLp-III cys /CT-apoA-I, lane 3: apoLp-III. Panel B: Size-exclusion chromatographic analysis. Protein (0.5 mg at a 1 mg/mL concentration) was applied to a <t>Superdex-200</t> column. Elution of the proteins was monitored at 210 nm using a flow rate of 0.5 mL/min. ApoLp-III eluted as a single peak at 17 mL (dash-dotted line), while apoA-I (solid line) and the chimera (dotted line) elute much earlier at 11 mL.
    Superdex 200, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 856 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superdex 200/product/GE Healthcare
    Average 99 stars, based on 856 article reviews
    Price from $9.99 to $1999.99
    superdex 200 - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    Purification of MTC28-BAP protein. Chromatogram showing (A) Elution profile of H10-T-MTC28-BAP protein on Ni Sepharose Fast Flow (NiFF) affinity column. Fraction numbers 6–17 were pooled (NiFF pool). (B) Elution profile of H10-T-MTC28-BAP protein on Superdex 75 gel-filtration column. Fraction numbers 16–24 were pooled (GFC pool). The GFC pool was treated with H6-TEV protease to cleave H10 tag from the protein followed by removal of cleaved tag and H6-TEV protease using Ni-affinity chromatography. (C) Elution profile of MTC28-BAP protein on Q Sepharose HP column. Fraction numbers 22–27 were pooled (QHP pool). (D) SDS-PAGE analysis of H10-T-MTC28-BAP protein at different stages during purification. The samples were analyzed by 0.1% SDS-12.5% PAGE under reducing conditions. The protein bands were visualized with Coomassie brilliant blue R-250 staining. Lane M, molecular weight marker, broad range (Bio-Rad, Hercules, CA) (shown in kDa); Lane 1, total cell after homogenization; Lane 2, High-High Speed Supernatant; Lane 3, NiFF pool; Lane 4, GFC pool; Lane 5, NiFF-TT pool (after desalting); Lane 6, QHP pool.

    Journal: PLoS ONE

    Article Title: Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection

    doi: 10.1371/journal.pone.0191315

    Figure Lengend Snippet: Purification of MTC28-BAP protein. Chromatogram showing (A) Elution profile of H10-T-MTC28-BAP protein on Ni Sepharose Fast Flow (NiFF) affinity column. Fraction numbers 6–17 were pooled (NiFF pool). (B) Elution profile of H10-T-MTC28-BAP protein on Superdex 75 gel-filtration column. Fraction numbers 16–24 were pooled (GFC pool). The GFC pool was treated with H6-TEV protease to cleave H10 tag from the protein followed by removal of cleaved tag and H6-TEV protease using Ni-affinity chromatography. (C) Elution profile of MTC28-BAP protein on Q Sepharose HP column. Fraction numbers 22–27 were pooled (QHP pool). (D) SDS-PAGE analysis of H10-T-MTC28-BAP protein at different stages during purification. The samples were analyzed by 0.1% SDS-12.5% PAGE under reducing conditions. The protein bands were visualized with Coomassie brilliant blue R-250 staining. Lane M, molecular weight marker, broad range (Bio-Rad, Hercules, CA) (shown in kDa); Lane 1, total cell after homogenization; Lane 2, High-High Speed Supernatant; Lane 3, NiFF pool; Lane 4, GFC pool; Lane 5, NiFF-TT pool (after desalting); Lane 6, QHP pool.

    Article Snippet: For H10-T-MTC28-BAP protein, gel-filtration chromatography of NiFF pool was performed on 480 ml Superdex 75 column (XK 26/100, GE Healthcare).

    Techniques: Purification, Flow Cytometry, Affinity Column, Filtration, Affinity Chromatography, SDS Page, Polyacrylamide Gel Electrophoresis, Staining, Molecular Weight, Marker, Homogenization

    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Article Snippet: Empty chromatography columns XK 26/40 (400 mm in length, 26 mm I.D., GE Healthcare, Piscataway, NJ) were packed with the anion exchange resin Q-sepharose XL (GE Healthcare).

    Techniques: Purification, Injection, Generated

    Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Article Snippet: Empty chromatography columns XK 26/40 (400 mm in length, 26 mm I.D., GE Healthcare, Piscataway, NJ) were packed with the anion exchange resin Q-sepharose XL (GE Healthcare).

    Techniques: Purification, Injection, Generated

    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Article Snippet: XK 26/40 columns (GE Healthcare) were packed with Q-sepharose XL resin (GE Healthcare) at room temperature using the ethanol-slurry-packing technique [ ].

    Techniques: Purification, Injection, Generated

    Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Article Snippet: XK 26/40 columns (GE Healthcare) were packed with Q-sepharose XL resin (GE Healthcare) at room temperature using the ethanol-slurry-packing technique [ ].

    Techniques: Purification, Injection, Generated

    Panel A: Non-denaturing PAGE analysis showing a much greater mobility of apoLp-III compared to apoA-I and the chimera. Twenty μg of protein was electrophoresed in the absence of SDS on a 4–20% Tris-glycine gel. Lane 1: apoA-I, lane 2: apoLp-III cys /CT-apoA-I, lane 3: apoLp-III. Panel B: Size-exclusion chromatographic analysis. Protein (0.5 mg at a 1 mg/mL concentration) was applied to a Superdex-200 column. Elution of the proteins was monitored at 210 nm using a flow rate of 0.5 mL/min. ApoLp-III eluted as a single peak at 17 mL (dash-dotted line), while apoA-I (solid line) and the chimera (dotted line) elute much earlier at 11 mL.

    Journal: Biochimica et biophysica acta

    Article Title: Transfer of C-terminal residues of human apolipoprotein A-I to insect apolipophorin III creates a two-domain chimeric protein with enhanced lipid binding activity

    doi: 10.1016/j.bbamem.2017.04.017

    Figure Lengend Snippet: Panel A: Non-denaturing PAGE analysis showing a much greater mobility of apoLp-III compared to apoA-I and the chimera. Twenty μg of protein was electrophoresed in the absence of SDS on a 4–20% Tris-glycine gel. Lane 1: apoA-I, lane 2: apoLp-III cys /CT-apoA-I, lane 3: apoLp-III. Panel B: Size-exclusion chromatographic analysis. Protein (0.5 mg at a 1 mg/mL concentration) was applied to a Superdex-200 column. Elution of the proteins was monitored at 210 nm using a flow rate of 0.5 mL/min. ApoLp-III eluted as a single peak at 17 mL (dash-dotted line), while apoA-I (solid line) and the chimera (dotted line) elute much earlier at 11 mL.

    Article Snippet: The protein solution was transferred to 6–8 kDa cut-off dialysis tubes (Spectrum laboratories, Rancho Dominguez, CA) and dialyzed against 4 L of 10 mM ammonium bicarbonate, pH 7.8 containing 1 mM EDTA with 3 additional buffer changes within 48 h. The recombinant proteins were further purified by size-exclusion chromatography using Superdex 200 (XK-26/70 column, GE Healthcare, Pittsburgh, PA).

    Techniques: Polyacrylamide Gel Electrophoresis, Concentration Assay, Flow Cytometry