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GE Healthcare xk 26 column
Xk 26 Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xk 26 column/product/GE Healthcare
Average 88 stars, based on 5 article reviews
Price from $9.99 to $1999.99
xk 26 column - by Bioz Stars, 2020-08
88/100 stars

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Plasmid Purification:

Article Title: Three Different Classes of Aminotransferases Evolved Prephenate Aminotransferase Functionality in Arogenate-competent Microorganisms *
Article Snippet: .. Crude extract was loaded onto 130 ml of EMD DEAE 650(M) resin (Merck) in an XK 26 column (26 × 260 mm2 , Amersham Biosciences) equilibrated with buffer A: 25 m m Hepes, pH 8.0, 1 m m EDTA, 1 m m DTT, 10% glycerol (v/v), 50 μ m PLP. ..

Flow Cytometry:

Article Title: A Plant-Produced Pfs230 Vaccine Candidate Blocks Transmission of Plasmodium falciparum ▿ ▿ †
Article Snippet: .. The filtrate was loaded onto an XK 26 column with Chelating Sepharose Big Beads resin (70-ml column volume [CV]) using the ÄKTA Purifier 100 (GE Healthcare, Piscataway, NJ) at a flow rate of 25 ml/min. ..

Fast Protein Liquid Chromatography:

Article Title: Structural Characterization of Proline-rich Tyrosine Kinase 2 (PYK2) Reveals a Unique (DFG-out) Conformation and Enables Inhibitor Design
Article Snippet: .. The resin was then transferred to an XK-26 column (Amersham Biosciences) connected to a FPLC™ and washed with buffer B until the OD reached 0.05 units. ..

Size-exclusion Chromatography:

Article Title: The cytosolic entry of diphtheria toxin catalytic domain requires a host cell cytosolic translocation factor complex
Article Snippet: .. Next, CTFs were fractionated by size exclusion chromatography using Sephacryl® S200 (Amersham Biosciences) XK 26 column (Amersham Biosciences) equilibrated with buffer B3. .. A Single Path Monitor (UV-1; Amersham Biosciences) was used to monitor chromatography.

Article Title: Nucleosome Recognition by the Piccolo NuA4 Histone Acetyltransferase Complex †
Article Snippet: .. Protein was further purified by size-exclusion chromatography on an XK-26 column packed with Sephracryl 200 (Amersham Biosciences) in 50 mM Tris at pH 7.5, 300 mM NaCl, 5 mM β -ME, and 1 mM ethylenediaminetetraacetic acid (EDTA). .. Fractions containing Esa1 based on SDS–PAGE and activity assays were pooled and concentrated.

Purification:

Article Title: Nucleosome Recognition by the Piccolo NuA4 Histone Acetyltransferase Complex †
Article Snippet: .. Protein was further purified by size-exclusion chromatography on an XK-26 column packed with Sephracryl 200 (Amersham Biosciences) in 50 mM Tris at pH 7.5, 300 mM NaCl, 5 mM β -ME, and 1 mM ethylenediaminetetraacetic acid (EDTA). .. Fractions containing Esa1 based on SDS–PAGE and activity assays were pooled and concentrated.

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  • 94
    GE Healthcare superdex 75 column
    Purification of MTC28-BAP protein. Chromatogram showing (A) Elution profile of H10-T-MTC28-BAP protein on Ni Sepharose Fast Flow (NiFF) affinity column. Fraction numbers 6–17 were pooled (NiFF pool). (B) Elution profile of H10-T-MTC28-BAP protein on <t>Superdex</t> 75 gel-filtration column. Fraction numbers 16–24 were pooled (GFC pool). The GFC pool was treated with H6-TEV protease to cleave H10 tag from the protein followed by removal of cleaved tag and H6-TEV protease using Ni-affinity chromatography. (C) Elution profile of MTC28-BAP protein on Q Sepharose HP column. Fraction numbers 22–27 were pooled (QHP pool). (D) SDS-PAGE analysis of H10-T-MTC28-BAP protein at different stages during purification. The samples were analyzed by 0.1% SDS-12.5% PAGE under reducing conditions. The protein bands were visualized with Coomassie brilliant blue R-250 staining. Lane M, molecular weight marker, broad range (Bio-Rad, Hercules, CA) (shown in kDa); Lane 1, total cell after homogenization; Lane 2, High-High Speed Supernatant; Lane 3, NiFF pool; Lane 4, GFC pool; Lane 5, NiFF-TT pool (after desalting); Lane 6, QHP pool.
    Superdex 75 Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 338 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superdex 75 column/product/GE Healthcare
    Average 94 stars, based on 338 article reviews
    Price from $9.99 to $1999.99
    superdex 75 column - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    85
    GE Healthcare xk 26 40 columns
    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.
    Xk 26 40 Columns, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xk 26 40 columns/product/GE Healthcare
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xk 26 40 columns - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    85
    GE Healthcare empty chromatography columns xk 26 40
    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.
    Empty Chromatography Columns Xk 26 40, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/empty chromatography columns xk 26 40/product/GE Healthcare
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    empty chromatography columns xk 26 40 - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Purification of MTC28-BAP protein. Chromatogram showing (A) Elution profile of H10-T-MTC28-BAP protein on Ni Sepharose Fast Flow (NiFF) affinity column. Fraction numbers 6–17 were pooled (NiFF pool). (B) Elution profile of H10-T-MTC28-BAP protein on Superdex 75 gel-filtration column. Fraction numbers 16–24 were pooled (GFC pool). The GFC pool was treated with H6-TEV protease to cleave H10 tag from the protein followed by removal of cleaved tag and H6-TEV protease using Ni-affinity chromatography. (C) Elution profile of MTC28-BAP protein on Q Sepharose HP column. Fraction numbers 22–27 were pooled (QHP pool). (D) SDS-PAGE analysis of H10-T-MTC28-BAP protein at different stages during purification. The samples were analyzed by 0.1% SDS-12.5% PAGE under reducing conditions. The protein bands were visualized with Coomassie brilliant blue R-250 staining. Lane M, molecular weight marker, broad range (Bio-Rad, Hercules, CA) (shown in kDa); Lane 1, total cell after homogenization; Lane 2, High-High Speed Supernatant; Lane 3, NiFF pool; Lane 4, GFC pool; Lane 5, NiFF-TT pool (after desalting); Lane 6, QHP pool.

    Journal: PLoS ONE

    Article Title: Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection

    doi: 10.1371/journal.pone.0191315

    Figure Lengend Snippet: Purification of MTC28-BAP protein. Chromatogram showing (A) Elution profile of H10-T-MTC28-BAP protein on Ni Sepharose Fast Flow (NiFF) affinity column. Fraction numbers 6–17 were pooled (NiFF pool). (B) Elution profile of H10-T-MTC28-BAP protein on Superdex 75 gel-filtration column. Fraction numbers 16–24 were pooled (GFC pool). The GFC pool was treated with H6-TEV protease to cleave H10 tag from the protein followed by removal of cleaved tag and H6-TEV protease using Ni-affinity chromatography. (C) Elution profile of MTC28-BAP protein on Q Sepharose HP column. Fraction numbers 22–27 were pooled (QHP pool). (D) SDS-PAGE analysis of H10-T-MTC28-BAP protein at different stages during purification. The samples were analyzed by 0.1% SDS-12.5% PAGE under reducing conditions. The protein bands were visualized with Coomassie brilliant blue R-250 staining. Lane M, molecular weight marker, broad range (Bio-Rad, Hercules, CA) (shown in kDa); Lane 1, total cell after homogenization; Lane 2, High-High Speed Supernatant; Lane 3, NiFF pool; Lane 4, GFC pool; Lane 5, NiFF-TT pool (after desalting); Lane 6, QHP pool.

    Article Snippet: For H10-T-MTC28-BAP protein, gel-filtration chromatography of NiFF pool was performed on 480 ml Superdex 75 column (XK 26/100, GE Healthcare).

    Techniques: Purification, Flow Cytometry, Affinity Column, Filtration, Affinity Chromatography, SDS Page, Polyacrylamide Gel Electrophoresis, Staining, Molecular Weight, Marker, Homogenization

    Elution profile of Superose 6 gel-filtration chromatography of fourfold-crystallized Ber e 2. 8 ml fractions of the major peak (excluding the front shoulder) were collected for crystallization trials.

    Journal:

    Article Title: Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2

    doi: 10.1107/S1744309107051445

    Figure Lengend Snippet: Elution profile of Superose 6 gel-filtration chromatography of fourfold-crystallized Ber e 2. 8 ml fractions of the major peak (excluding the front shoulder) were collected for crystallization trials.

    Article Snippet: To further purify the four-times crystallized Ber e 2, a 300 ml Superose 6 column (XK 26/70, GE Healthcare, Piscataway, NJ, USA) was used.

    Techniques: Filtration, Chromatography, Crystallization Assay

    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Article Snippet: XK 26/40 columns (GE Healthcare) were packed with Q-sepharose XL resin (GE Healthcare) at room temperature using the ethanol-slurry-packing technique [ ].

    Techniques: Purification, Injection, Generated

    Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Article Snippet: XK 26/40 columns (GE Healthcare) were packed with Q-sepharose XL resin (GE Healthcare) at room temperature using the ethanol-slurry-packing technique [ ].

    Techniques: Purification, Injection, Generated

    Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified hHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of hRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the hHb peak, while the “2” represents the impurity peak.

    Article Snippet: Empty chromatography columns XK 26/40 (400 mm in length, 26 mm I.D., GE Healthcare, Piscataway, NJ) were packed with the anion exchange resin Q-sepharose XL (GE Healthcare).

    Techniques: Purification, Injection, Generated

    Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    Article Title: Preparation of Ultrapure Bovine and Human Hemoglobin by Anion Exchange Chromatography

    doi: 10.1016/j.jchromb.2008.02.014

    Figure Lengend Snippet: Chromatogram of purified bHb Column: XK 26/40 (400 mm in length, 26 mm I.D.) was packed with 120 mL Q-sepharose XL. Injection: 50 mL of bRBC lysate was introduced into the column via Superloop. Elution: a linear gradient was generated by changing from 100% buffer A to 75% buffer B in 5 CVs (100 min). This was followed by a step gradient of 100% buffer B. The “1” in this Figure represents the bHb peak, while the “2” represents the impurity peak.

    Article Snippet: Empty chromatography columns XK 26/40 (400 mm in length, 26 mm I.D., GE Healthcare, Piscataway, NJ) were packed with the anion exchange resin Q-sepharose XL (GE Healthcare).

    Techniques: Purification, Injection, Generated